CN117158420A - Parasitic wasp attractant for tea garden - Google Patents
Parasitic wasp attractant for tea garden Download PDFInfo
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- 239000005667 attractant Substances 0.000 title claims abstract description 45
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- 239000000284 extract Substances 0.000 claims abstract description 36
- QMVPMAAFGQKVCJ-UHFFFAOYSA-N citronellol Chemical compound OCCC(C)CCC=C(C)C QMVPMAAFGQKVCJ-UHFFFAOYSA-N 0.000 claims abstract description 30
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- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 28
- 239000001110 calcium chloride Substances 0.000 claims abstract description 27
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 27
- 201000010099 disease Diseases 0.000 claims abstract description 23
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 23
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims abstract description 20
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims abstract description 20
- 229940092665 tea leaf extract Drugs 0.000 claims abstract description 19
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 16
- 239000000661 sodium alginate Substances 0.000 claims abstract description 16
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 16
- GEWDNTWNSAZUDX-WQMVXFAESA-N (-)-methyl jasmonate Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-WQMVXFAESA-N 0.000 claims abstract description 15
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 claims abstract description 15
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 claims abstract description 15
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 claims abstract description 15
- 229920002498 Beta-glucan Polymers 0.000 claims abstract description 15
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims abstract description 15
- 229920001543 Laminarin Polymers 0.000 claims abstract description 15
- 239000005717 Laminarin Substances 0.000 claims abstract description 15
- 240000003553 Leptospermum scoparium Species 0.000 claims abstract description 15
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 15
- JGQFVRIQXUFPAH-UHFFFAOYSA-N beta-citronellol Natural products OCCC(C)CCCC(C)=C JGQFVRIQXUFPAH-UHFFFAOYSA-N 0.000 claims abstract description 15
- RRGOKSYVAZDNKR-ARJAWSKDSA-M cis-3-hexenylacetate Chemical compound CC\C=C/CCCC([O-])=O RRGOKSYVAZDNKR-ARJAWSKDSA-M 0.000 claims abstract description 15
- 235000000484 citronellol Nutrition 0.000 claims abstract description 15
- 229930002839 ionone Natural products 0.000 claims abstract description 15
- 150000002499 ionone derivatives Chemical class 0.000 claims abstract description 15
- 229930007744 linalool Natural products 0.000 claims abstract description 15
- GEWDNTWNSAZUDX-UHFFFAOYSA-N methyl 7-epi-jasmonate Natural products CCC=CCC1C(CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-UHFFFAOYSA-N 0.000 claims abstract description 15
- NODGRWCMFMEGJH-UHFFFAOYSA-N p-ethylacetophenone Chemical compound CCC1=CC=C(C(C)=O)C=C1 NODGRWCMFMEGJH-UHFFFAOYSA-N 0.000 claims abstract description 15
- NPFVOOAXDOBMCE-UHFFFAOYSA-N trans-3-hexenyl acetate Natural products CCC=CCCOC(C)=O NPFVOOAXDOBMCE-UHFFFAOYSA-N 0.000 claims abstract description 15
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- 239000000725 suspension Substances 0.000 claims description 11
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- 238000004108 freeze drying Methods 0.000 claims description 9
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 241000238631 Hexapoda Species 0.000 description 8
- 239000000575 pesticide Substances 0.000 description 7
- 241000607479 Yersinia pestis Species 0.000 description 4
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- 235000013601 eggs Nutrition 0.000 description 3
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- 210000003608 fece Anatomy 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000000447 pesticide residue Substances 0.000 description 2
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- 241001033777 Brachymeria lasus Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The application belongs to the technical field of agricultural preparation, and relates to a parasitic wasp attractant for tea gardens. The parasitic wasp attractant for the tea garden comprises the following components in parts by mass: beta-citronellol, ionone, (Z) -3-hexenyl acetate, linalool, methyl jasmonate, beta-1, 3-glucan laminarin, 4' -ethyl acetophenone, tea leaf moth extract, disease tea leaf extract, hydroxyapatite, sodium alginate and calcium chloride. The aim of attracting parasitic bees to increase the quantity of the parasitic bees in the tea garden is achieved by increasing the concentration of the attracting substances for the parasitic bees in the tea garden, and the slow release effect can increase the volatilization time of the attractant and acts on the field for a long time. The tea cereal moth extract and the disease tea tree leaf extract not only can promote the attracting effect, but also can promote the selectivity of attracting parasitic bees.
Description
Technical Field
The application belongs to the technical field of agricultural preparation, and particularly relates to a parasitic wasp attractant for tea gardens.
Background
The tea moth (Agriophara rhombata meyr.) is also known as the tea moth, belonging to the family of lepidoptera. The main mouth is She Helao leaves, and part of the initially hatched larvae can also be damaged by tender tips. When the tea leaves are large, the tea leaves are all eaten with light, even the bark is gnawed, so that the branches die, meanwhile, the upper part and the lower part of the tree are full of the insect manure, the insect manure is adhered to the trimmed branches and leaves, and the quality of the crude tea is affected.
The tea moth larvae have strong concealment and are concealed in She Bao to eat, and immediately fall into the ground or other concealed places after being frightened or disturbed. Adults are allowed to travel between the plexus, to cross at night, and have no phototaxis, are not good for flying, and can lay more than 200 eggs per female, and spread leaf backs. The larvae bite the leaves in the folded leaves, only damage tea, have strong hunger resistance and pupate in the buds, on the leaves or on the soil surface dead branches after the larvae are slightly mature.
The existing control method comprises the control measures of artificial insect harvesting, artificial pesticide bud cutting, pesticide control and the like. Although the chemical pesticide has quick effect, the application of the chemical pesticide not only causes pesticide residue and serious environmental pollution, but also easily kills natural enemies of the thea-valley moths, and the thea-valley moths have stronger drug resistance to various chemical pesticides, so the chemical pesticide does not meet the requirements of sustainability and ecological control of pest control.
Natural enemies are one of factors restricting the occurrence of the theasitophila, and the larva stage is often parasitic by the trypanosome (Asympiesiella sp.) with the parasitic rate reaching more than 20 percent. Also a kind of parasitic larva, a broad legged wasp of parasitic pupa (Brachymeria Lasus Walker), trichogramma (Trichogramma evanescens Westwood), and parasitic fly (Tachinidae), etc. The biological control is safe to people and animals, has no environmental pollution, and can not generate drug resistance and continuous control of pests, so the biological control is an important way for the tea moth. However, under natural conditions, the number of parasitic bees in the tea garden is insufficient to cope with the insect pest of the tea moth, so how to increase the number of parasitic bees in the tea garden is a problem which is urgent to be solved by those skilled in the art.
Disclosure of Invention
In order to solve the technical problems, the application provides a parasitic wasp attractant for tea gardens. The aim of attracting parasitic bees to increase the quantity of the parasitic bees in the tea garden is achieved by increasing the concentration of the attracting substances for the parasitic bees in the tea garden, and the slow release effect can increase the volatilization time of the attractant and acts on the field for a long time. The tea cereal moth extract and the disease tea tree leaf extract not only can promote the attracting effect, but also can promote the selectivity of attracting parasitic bees.
In order to achieve the above purpose, the present application provides the following technical solutions:
one of the technical schemes of the application is as follows: the parasitic wasp attractant for the tea garden comprises the following components in parts by mass:
0.5 to 1 part of beta-citronellol, 1 to 2 parts of ionone, 0.5 to 1 part of (Z) -3-hexenyl acetate, 0.5 to 1 part of linalool, 2 to 3 parts of methyl jasmonate, 5 to 8 parts of beta-1, 3-glucan laminarin, 0.5 to 1 part of 4' -ethylacetophenone, 0.2 to 0.4 part of tea moth extract, 0.2 to 0.4 part of disease tea leaf extract, 30 to 50 parts of hydroxyapatite, 3 to 8 parts of sodium alginate and 10 to 15 parts of calcium chloride.
Further, the parasitic wasp attractant for the tea garden comprises the following components in parts by mass:
1 part of beta-citronellol, 2 parts of ionone, 0.5 part of (Z) -3-hexenyl acetate, 0.5 part of linalool, 3 parts of methyl jasmonate, 5 parts of beta-1, 3-glucan laminarin, 1 part of 4' -ethyl acetophenone, 0.4 part of tea moth extract, 0.4 part of disease tea leaf extract, 40 parts of hydroxyapatite, 5 parts of sodium alginate and 15 parts of calcium chloride.
Further, the tea-moth extract is prepared from tea-moth eggs, tea-moth larvae, tea-moth adults and/or tea-moth cocoons by an alcohol extraction method.
Further, the disease tea leaf extract is prepared from tea tree leaves invaded by tea moth larvae by an alcohol extraction method.
The alcohol extraction method in the preparation of the tea moth extract and the disease tea leaf extract in the application is a conventional alcohol extraction method.
Further, the particle size of the hydroxyapatite is 100 to 500 μm.
The feeding behavior of the phytophagous insects on the plants can change the secondary metabolic process of the plants, the composition and the proportion of volatile substances of the plants can be changed, and the parasitic wasp search can be further guided positively; meanwhile, the volatile matters released by feeding or harmful host larvae play a forward guiding role in searching the parasitic wasps, so that the attractiveness of the attractant to the parasitic wasps can be increased by adding the tea moth extract and the disease tea tree leaf extract, the quantity of the parasitic wasps in a tea garden is improved, and the purpose of treating the tea moth is achieved. Wherein, too large particle size of the hydroxyapatite can reduce the release control rate of volatile components, so as to reduce the attracting effect, and too low particle size of the hydroxyapatite can improve the release control rate of volatile components, so as to reduce the action days of the attracting effect.
The second technical scheme of the application is as follows: the preparation method of the parasitic wasp attractant for the tea garden comprises the following steps:
mixing beta-citronellol, ionone, (Z) -3-hexenyl acetate, linalool, methyl jasmonate, beta-1, 3-glucan laminarin, 4' -ethyl acetophenone, tea cereal moth extract and disease tea tree leaf extract, and adding 2-3 times of water to prepare a suspension;
adding hydroxyapatite into the suspension to perform adsorption reaction, and then adding sodium alginate and uniformly stirring to obtain adsorption solution;
preparing calcium chloride into calcium chloride aqueous solution, then dripping adsorption liquid into the calcium chloride aqueous solution at a constant speed, and collecting a solid part;
and freeze-drying and sealing the solid part to obtain the parasitic wasp attractant for tea gardens.
Further, the time of the adsorption reaction is 2-4 hours, and the adsorption reaction is stirred for 30-40 seconds every 30-40 minutes.
Further, the concentration of the calcium chloride aqueous solution is 1 to 1.5wt.%.
Further, the temperature of the freeze drying is-20 to-10 ℃ and the pressure is 2 to 4Pa.
The third technical scheme of the application: provides an application of the parasitic wasp attractant for the tea garden in the treatment of the tea moth in the tea garden.
Further, the application method comprises the following steps: and loading the parasitic wasp attractant into a non-woven fabric bag and suspending the parasitic wasp attractant in the middle of tea tree branches.
Preferably, the loading amount of the parasitic wasp attractant is 50-100 g.
Compared with the prior art, the technical scheme has the following beneficial effects:
the parasitic wasp attractant prepared by the application prolongs the attracting time through slow release, achieves the purpose of increasing the number of parasitic wasps in the field through long-time acting on the field, and achieves the purpose of accurately controlling the insect pests of the theast moth through increasing the number of the parasitic wasps.
The parasitic wasp attractant prepared by the application is added with the tea moth extract and the disease tea leaf extract, so that the attracting effect can be improved, and the selectivity of the attractant to parasitic wasps can be improved.
The parasitic wasp attractant prepared by the application can reduce the pesticide application amount of tea gardens after application, effectively reduce the pesticide residue of tea, and accords with the development expectations of green agriculture.
The parasitic wasp attractant prepared by the application can reduce the workload of artificial insect collection and artificial insect bud clipping after application, so as to further reduce the labor cost of tea tree management.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application. In the drawings:
fig. 1 is a graph showing comparison of data of attracting parasitic wasps with the parasitic wasp attractants for tea gardens prepared in example 1 and comparative examples 1 to 4.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The preparation method of the tea-cereal moth extract comprises the following steps:
crushing the thea moth eggs, thea moth larvae, thea moth adults and/or thea moth cocoons, adding 12 times of ethanol water solution for leaching extraction, repeating the extraction step for 4 times, wherein the mass fraction of the ethanol water solution is 70%, the extraction temperature is 60 ℃, combining the extraction solutions, removing ethanol, and freeze-drying to obtain the thea moth extract.
The preparation method of the disease tea leaf extract comprises the following steps:
crushing tea leaves invaded by the tea moth larvae, adding 12 times of ethanol water solution for leaching extraction, repeating the extraction step for 4 times, wherein the mass fraction of the ethanol water solution is 70%, the extraction temperature is 60 ℃, combining the extracts, removing ethanol, and freeze-drying to obtain the harmful tea leaf extract.
Example 1
Preparation of parasitic wasp attractant for tea garden:
s1, weighing 1 part of beta-citronellol, 2 parts of ionone, 0.5 part of (Z) -3-hexenyl acetate, 0.5 part of linalool, 3 parts of methyl jasmonate, 5 parts of beta-1, 3-glucan laminarin, 1 part of 4' -ethyl acetophenone, 0.4 part of tea leaf extract, 0.4 part of disease tea leaf extract, 40 parts of hydroxyapatite with the particle size of 500 mu m, 5 parts of sodium alginate and 15 parts of calcium chloride according to mass parts;
s2, mixing beta-citronellol, ionone, (Z) -3-hexenyl acetate, linalool, methyl jasmonate, beta-1, 3-glucan laminarin, 4' -ethyl acetophenone, a tea cereal moth extract and a disease tea tree leaf extract, and adding 2 times of water to prepare a suspension;
s3, adding hydroxyapatite into the suspension to perform adsorption reaction for 3 hours, stirring for 30 seconds every 30 minutes during the adsorption reaction, and then adding sodium alginate to uniformly stir to obtain adsorption solution;
s4, preparing calcium chloride into a calcium chloride aqueous solution with the concentration of 1wt.%, then dropwise adding the adsorption solution into the calcium chloride aqueous solution at a constant speed, and collecting a solid part;
s5, freeze-drying the solid part at the temperature of-20 ℃ and the pressure of 2Pa, and sealing after the drying is finished to obtain the parasitic wasp attractant for tea gardens.
Example 2
Preparation of parasitic wasp attractant for tea garden:
s1, weighing 0.5 part of beta-citronellol, 1 part of ionone, 0.5 part of (Z) -3-hexenyl acetate, 0.5 part of linalool, 2 parts of methyl jasmonate, 5 parts of beta-1, 3-glucan laminarin, 0.5 part of 4' -ethylacetophenone, 0.2 part of tea leaf extract, 0.2 part of disease tea leaf extract, 30 parts of hydroxyapatite with the particle size of 100 mu m, 2 parts of sodium alginate and 10 parts of calcium chloride for later use;
s2, mixing beta-citronellol, ionone, (Z) -3-hexenyl acetate, linalool, methyl jasmonate, beta-1, 3-glucan laminarin, 4' -ethyl acetophenone, a tea cereal moth extract and a disease tea tree leaf extract, and adding 2 times of water to prepare a suspension;
s3, adding hydroxyapatite into the suspension to perform adsorption reaction for 2 hours, stirring for 30 seconds every 30 minutes during the adsorption reaction, and then adding sodium alginate to uniformly stir to obtain adsorption solution;
s4, preparing calcium chloride into a calcium chloride aqueous solution with the concentration of 1wt.%, then dropwise adding the adsorption solution into the calcium chloride aqueous solution at a constant speed, and collecting a solid part;
s5, freeze-drying the solid part at the temperature of-20 ℃ and the pressure of 2Pa, and sealing after the drying is finished to obtain the parasitic wasp attractant for tea gardens.
Example 3
Preparation of parasitic wasp attractant for tea garden:
s1, weighing 1 part of beta-citronellol, 2 parts of ionone, 1 part of (Z) -3-hexenyl acetate, 1 part of linalool, 3 parts of methyl jasmonate, 8 parts of beta-1, 3-glucan laminarin, 1 part of 4' -ethyl acetophenone, 0.3 part of tea moth extract, 0.3 part of disease tea leaf extract, 50 parts of hydroxyapatite with the particle size of 300 mu m, 8 parts of sodium alginate and 15 parts of calcium chloride according to mass parts for standby;
s2, mixing beta-citronellol, ionone, (Z) -3-hexenyl acetate, linalool, methyl jasmonate, beta-1, 3-glucan laminarin, 4' -ethyl acetophenone, a tea cereal moth extract and a disease tea tree leaf extract, and adding 3 times of water to prepare a suspension;
s3, adding hydroxyapatite into the suspension to perform adsorption reaction for 4 hours, stirring for 40 seconds every 40 minutes during the adsorption reaction, and then adding sodium alginate to uniformly stir to obtain adsorption solution;
s4, preparing calcium chloride into a calcium chloride aqueous solution with the concentration of 1.5wt.%, then dropwise adding the adsorption solution into the calcium chloride aqueous solution at a constant speed, and collecting a solid part;
s5, freeze-drying the solid part at-10 ℃ and under the pressure of 4Pa, and sealing after the drying is finished to obtain the parasitic wasp attractant for tea gardens.
Comparative example 1
Preparation of parasitic wasp attractant for tea garden:
s1, weighing 1 part of beta-citronellol, 2 parts of ionone, 0.5 part of (Z) -3-hexenyl acetate, 0.5 part of linalool, 3 parts of methyl jasmonate, 5 parts of beta-1, 3-glucan laminarin, 1 part of 4' -ethyl acetophenone, 0.4 part of tea moth extract and 0.4 part of disease tea leaf extract according to mass parts for standby;
s2, mixing beta-citronellol, ionone, (Z) -3-hexenyl acetate, linalool, methyl jasmonate, beta-1, 3-glucan laminarin, 4' -ethyl acetophenone, tea cereal moth extract and disease tea tree leaf extract, and adding 2 times of water to prepare a suspension, namely the parasitic wasp attractant for tea gardens.
Comparative example 2
The difference from example 1 is that the thea-glutinosa moth extract was not added.
Comparative example 3
The only difference compared to example 1 is that no diseased tea leaf extract was added.
Comparative example 4
The difference compared to example 1 is only that the tea moth extract and the diseased tea tree leaf extract are not added.
Test examples
25 unidirectional beehives are manufactured and divided into 5 groups of 5 beehives, and parasitic wasp attractants for tea gardens, which are prepared in example 1 and comparative examples 1-4 and have the same dosage, are respectively placed in the beehives.
Wherein the dosage refers to the sum of the mass of other substances after the removal of the hydroxyapatite, the sodium alginate and the calcium chloride.
In the middle of July, the beehive is placed in 25 tea garden fields which are not interfered with each other, and the hanging height is 0.5m.
The number of parasitic wasps in each group of five beehives is counted at six pm every day, an average value is obtained, and the parasitic bees in the beehives are emptied, and the counted data are shown in figure 1.
As can be seen from fig. 1, the parasitic wasp attractant for tea garden prepared in comparative example 1 does not use hydroxyapatite, sodium alginate and calcium chloride, and the amount of parasitic wasps attracted is high in the early stage of application, the volatile amount of active ingredients is high in the early stage, and the amount attracted per day drops sharply in about 9 days of application, because the parasitic wasp attractant for tea garden has insufficient components with attracting effect, and the volatile amount is insufficient to attract parasitic wasps; as can be seen from the data of the example 1 and the comparative examples 2 to 4, the parasitic wasp attractant for tea gardens prepared in the example 1 and the comparative examples 2 to 4 uses hydroxyapatite, sodium alginate and calcium chloride components, so that the parasitic wasp attractant for tea gardens prepared in the application still has good attracting effect after being used for 30 days, and the parasitic wasp attractant for tea gardens prepared in the application has good slow release effect; as can be seen from the data of comparative examples 1 and 2 to 4, the addition of the tea leaf extract and the disease tea leaf extract has a good promoting effect on the attraction of parasitic wasps.
Further, as a result of analyzing the types of parasitic bees attracted in example 1 and comparative examples 1 to 4, it was found that the amounts of parasitic bees attracted in example 1 and comparative examples 1 to 3 were able to act on the theast moth higher than those in comparative example 4, and it was found that the selectivity of the parasitic bee attractant could be increased when theast moth extract and diseased tea tree leaf extract were added.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. The parasitic wasp attractant for the tea garden is characterized by comprising the following components in parts by mass:
0.5 to 1 part of beta-citronellol, 1 to 2 parts of ionone, 0.5 to 1 part of (Z) -3-hexenyl acetate, 0.5 to 1 part of linalool, 2 to 3 parts of methyl jasmonate, 5 to 8 parts of beta-1, 3-glucan laminarin, 0.5 to 1 part of 4' -ethylacetophenone, 0.2 to 0.4 part of tea moth extract, 0.2 to 0.4 part of disease tea leaf extract, 30 to 50 parts of hydroxyapatite, 3 to 8 parts of sodium alginate and 10 to 15 parts of calcium chloride.
2. The parasitic wasp attractant for tea gardens as claimed in claim 1, comprising the following components in parts by mass:
1 part of beta-citronellol, 2 parts of ionone, 0.5 part of (Z) -3-hexenyl acetate, 0.5 part of linalool, 3 parts of methyl jasmonate, 5 parts of beta-1, 3-glucan laminarin, 1 part of 4' -ethyl acetophenone, 0.4 part of tea moth extract, 0.4 part of disease tea leaf extract, 40 parts of hydroxyapatite, 5 parts of sodium alginate and 15 parts of calcium chloride.
3. The parasitic wasp attractant for tea garden according to any one of claims 1 to 2, wherein the particle size of the hydroxyapatite is 100 to 500 μm.
4. A method of preparing a parasitic wasp attractant for tea gardens as claimed in any one of claims 1 to 3, comprising the steps of:
mixing beta-citronellol, ionone, (Z) -3-hexenyl acetate, linalool, methyl jasmonate, beta-1, 3-glucan laminarin, 4' -ethyl acetophenone, tea cereal moth extract and disease tea tree leaf extract, and adding 2-3 times of water to prepare a suspension;
adding hydroxyapatite into the suspension to perform adsorption reaction, and then adding sodium alginate and uniformly stirring to obtain adsorption solution;
preparing the calcium chloride into a calcium chloride aqueous solution, then dropwise adding the adsorption liquid into the calcium chloride aqueous solution at a constant speed, and collecting a solid part;
and freeze-drying and sealing the solid part to obtain the parasitic wasp attractant for tea gardens.
5. The method according to claim 4, wherein the adsorption reaction is carried out for 2 to 4 hours while stirring for 30 to 40 seconds every 30 to 40 minutes.
6. The method according to claim 4, wherein the concentration of the aqueous calcium chloride solution is 1 to 1.5wt.%.
7. The method according to claim 4, wherein the freeze-drying temperature is-20 to-10℃and the pressure is 2 to 4Pa.
8. Use of a parasitic wasp attractant as claimed in any one of claims 1 to 3 in the treatment of tea leaf moths in a tea garden.
9. The use according to claim 8, wherein the parasitic wasp attractant is suspended in the middle of tea tree branches after being packed in a non-woven bag.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111226931A (en) * | 2020-03-20 | 2020-06-05 | 上海市农业科学院 | Ionone parasitic wasp attractant, preparation method and application |
| CN112889816A (en) * | 2021-01-07 | 2021-06-04 | 仲恺农业工程学院 | Insect virus and sex pheromone microcapsule and preparation method thereof |
| CN116267924A (en) * | 2023-03-24 | 2023-06-23 | 云南省农业科学院茶叶研究所 | Parasitic wasp attractant for removing insect pest tea moth of tea tree and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111226931A (en) * | 2020-03-20 | 2020-06-05 | 上海市农业科学院 | Ionone parasitic wasp attractant, preparation method and application |
| CN112889816A (en) * | 2021-01-07 | 2021-06-04 | 仲恺农业工程学院 | Insect virus and sex pheromone microcapsule and preparation method thereof |
| CN116267924A (en) * | 2023-03-24 | 2023-06-23 | 云南省农业科学院茶叶研究所 | Parasitic wasp attractant for removing insect pest tea moth of tea tree and preparation method thereof |
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