CN117136788A - Morchella strain preparation method - Google Patents
Morchella strain preparation method Download PDFInfo
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- CN117136788A CN117136788A CN202311316889.XA CN202311316889A CN117136788A CN 117136788 A CN117136788 A CN 117136788A CN 202311316889 A CN202311316889 A CN 202311316889A CN 117136788 A CN117136788 A CN 117136788A
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- 241000221638 Morchella Species 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 40
- 238000011081 inoculation Methods 0.000 claims abstract description 37
- 230000001954 sterilising effect Effects 0.000 claims abstract description 14
- 238000011049 filling Methods 0.000 claims abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000007789 sealing Methods 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 14
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 238000009331 sowing Methods 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 5
- 235000012255 calcium oxide Nutrition 0.000 claims description 4
- 239000000292 calcium oxide Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000000428 dust Substances 0.000 claims description 4
- 229910052602 gypsum Inorganic materials 0.000 claims description 4
- 239000010440 gypsum Substances 0.000 claims description 4
- 239000010903 husk Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 208000015181 infectious disease Diseases 0.000 abstract description 5
- 241000233866 Fungi Species 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 238000004904 shortening Methods 0.000 abstract description 2
- 241000209094 Oryza Species 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006957 competitive inhibition Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/65—Cultivation containers; Lids therefor characterised by the lids, e.g. lids with filters
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The application relates to the technical field of edible fungus culture, and discloses a manufacturing method of Morchella strains, which comprises the following steps: s1 preparation, S2 mixing, S3 charging, S4 sterilization, S5 inoculation and S6 culture. By reserving the strip-shaped holes in the culture medium and filling the strip-shaped holes with the strain of the previous stage, the growth path of the mycelia covered with the whole culture container can be shortened while the density of the strain is improved, so that the mycelia can grow into the whole culture container more quickly, the culture period of the mycelia growth is shortened, and the development of the miscellaneous bacteria is restrained in time and space. The application has the effects of shortening the culture period of hypha growth and reducing the infection rate of mixed bacteria.
Description
Technical Field
The application relates to the technical field of edible fungus culture, in particular to a method for manufacturing Morchella strains.
Background
Morchella strain is the most basic production data in Morchella production, and plays an important role in determining the yield and quality of Morchella. Morchella strains are divided into first-level strains (also called mother strains or test tube strains), second-level strains (also called stock strains), and third-level strains (also called cultivars or production strains). Stock is typically used to cultivate stock and then inoculated into a culture medium for growth into cultivars for planting. Morchella is planted in the autumn and winter every year, land preparation and sowing are needed, and harvesting is carried out in spring, but the growth of Morchella is greatly influenced by temperature, the color and the mushroom shape of the fruiting body can be influenced by overhigh temperature, and once the temperature in a greenhouse exceeds 25 ℃, the Morchella can be directly killed, and fruiting bodies can not be harvested continuously. Therefore, it is very advantageous to be able to shorten the fruiting time of morchella for improving the yield.
The inoculation method is generally adopted, wherein the strain of the upper stage is poured on the surface of a culture medium, and the strain is grown and cultured from top to bottom. The strain diffuses to the whole culture container in a longer path, and the problem of long growth period exists.
The conventional Morchella strain culture method can refer to patent document with publication number CN109247192A, and the patent document discloses a Morchella culture method, and the culture period of the strain is shortened by adopting a method of cross inoculation of hyphae on the horizontal surface of a culture medium. However, as in the conventional inoculation mode, the strain is propagated from top to bottom from the bottle mouth to the bottle bottom, and a longer path is also required.
Disclosure of Invention
The application provides a preparation method of Morchella strain for shortening the culture period of mycelium growth and reducing the infection rate of mixed bacteria.
The preparation method of Morchella strain provided by the application adopts the following technical scheme:
a preparation method of Morchella strains comprises the following steps:
s1, preparing: preparing raw materials required by a culture medium, and soaking and rehydrating the dried plant raw materials, wherein the rehydration rate is 1:1.5;
s2, mixing: mixing the raw materials prepared in the step S1 according to a proportion to obtain a culture medium;
s3, charging: providing a culture container and an inoculating rod, filling a part of the culture medium into the culture container, stopping filling when the top surface of the culture medium is 3-6 cm away from the mouth of the culture container, inserting the inoculating rod into the culture container, filling the rest culture medium into the culture container, and sealing the culture container;
s4, sterilization: sterilizing the culture container filled with the culture medium for 4-6 hours at the temperature of 123-126 ℃ and cooling;
s5, inoculation: in a ten-thousand-level aseptic chamber or a purifying inoculation assembly line, a strip-shaped hole is formed in the center of a culture medium after the inoculation rod is pulled out, and a strain of the previous stage is placed in the strip-shaped hole and fills the strip-shaped hole;
s6, culturing: culturing strain in a hundred thousand-level clean room, and culturing at 12-20deg.C for 25-40 days until the culture container is full of mycelia for inoculation or sowing at the next stage.
By adopting the technical scheme, the upper-level strain is filled in the strip-shaped holes, so that the strain can simultaneously grow in a divergent manner from the middle to the outside along the height direction of the culture container, and the growth path of the mycelium which is fully distributed in the whole culture container can be shortened. Compared with the traditional inoculation mode, the method has the advantages that the density of the strain is improved, the growth path is shortened, the whole culture container is fully grown by hyphae faster, and the culture period of hyphae growth is shortened. The growth of microorganisms has a competitive inhibition mechanism, and Morchella strains rapidly develop, so that the development of mixed bacteria can be inhibited in time and space, and the probability of mixed bacteria infection is reduced as much as possible.
Further, a sealing cover is arranged at the mouth part of the culture container, an air hole is formed in the sealing cover, the inoculating rod is in a thin-wall tubular shape, the sealing cover can be detachably connected with the inoculating rod, and in the step S3, when the sealing cover is used for sealing the culture container, the inoculating rod is opposite to the air hole and is fixedly connected with the sealing cover; when inoculation in the step S5 is carried out, the strain of the previous stage is directly put into the culture container from the orifice of the inoculation rod; and pulling out the inoculating rod after the inoculation is completed.
By adopting the technical scheme, the inoculation rod can provide support for the culture medium in the culture vessel between the step S3 of loading and the step S5 of inoculation, and the bar shape Kong Takong during the step S4 sterilization is prevented, so that the integrity of the bar shape hole during inoculation is ensured. The inoculating rod is made into a hollow thin-wall tube shape, the upper-level strain can be put into the bar-shaped hole in the inoculating rod tube through the air hole on the bottle cover without opening the bottle cover, the inoculating rod can play a role in protecting the wall of the culture medium, and the bar-shaped hole is prevented from collapsing due to improper operation or vibration in the inoculating process. Finally, the inoculating rod is taken out again, so that the operation steps can be simplified.
Further, the sealing cover is detachably connected with a breathable fungus-isolating film, and the breathable fungus-isolating film is used for sealing the air holes.
By adopting the technical scheme, the breathable bacteria-isolating film can enable the sealed culture container to be breathable and isolate mixed bacteria, so that microorganisms such as external bacteria are difficult to enter the culture container, and the ventilation can ensure sufficient oxygen supply required by hypha growth.
Further, a plurality of clamping parts are arranged on the inner side of the sealing cover around the air hole, a clamping groove with a downward notch is formed in the clamping part, and the inoculating rod is in interference fit with the clamping groove.
By adopting the technical scheme, the clamping part is arranged at the inner side of the sealing cover, the clamping groove is in interference fit with the inoculating rod, the clamping groove plays a role in positioning, and the inoculating rod is kept vertically and stably positioned at the center of the culture container, so that the shape of the strip-shaped hole is regular, and the position is fixed; because the surface of the inoculating rod is smooth and straight, the inoculating rod is inconvenient to take out, and the sealing cover is in interference fit with the inoculating rod, so that the inoculating rod can be synchronously pulled out when the sealing cover is opened, and the operation of pulling out the inoculating rod is simpler.
Further, in the steps S1 and S2, the weight ratio of the raw materials of the culture medium is as follows: 50-60% of wet wheat grains, 27-37% of wet rice husks, 10% of wet wood dust, 2% of quicklime and 1% of gypsum powder.
By adopting the technical scheme, the raw materials are easy to obtain, the cost is low, and the wet environment is conducive to hypha growth.
Further, in the step S3 of charging, the weight of each part of culture medium is 400g-1kg.
By adopting the technical scheme, experiments prove that the culture medium can be fully utilized when the weight of each culture medium is 400g-1kg.
Further, when the stock seed is manufactured, the strain of the previous stage is a test tube seed; when the cultivated species is produced, the strain of the previous stage is the stock species.
By adopting the technical scheme, the method is suitable for manufacturing various strains and can be widely applied to manufacturing various strains.
In summary, the present application includes at least one of the following beneficial technical effects:
1. the bar-shaped holes are reserved in the culture medium, and the bar-shaped holes are filled with the strain of the previous stage, so that the growth path of the whole culture container filled with the mycelium can be shortened while the density of the strain is improved, the whole culture container is more quickly filled with the mycelium, the culture period of mycelium growth is shortened, the development of the mixed bacteria is restrained in time and space, and the probability of mixed bacteria infection can be reduced.
2. The inoculating rod is made into a hollow thin-wall tube shape, the upper level strain is put into the strip-shaped hole from the orifice of the inoculating rod, the inoculating rod can play a role of protecting the wall of the culture medium, and the strip-shaped hole is prevented from collapsing due to improper operation or vibration in the inoculating process; pulling out the inoculating rod after inoculation is completed can simplify the operation steps.
3. Through setting up joint portion in sealed lid inboard to make draw-in groove and inoculation stick interference fit, play the locate action to the inoculation stick, thereby guarantee bar hole shape rule, fixed in position, can also pull out the inoculation stick when opening sealed lid, overcome the smooth straight shortcoming of inconvenient taking out in inoculation stick surface, make the operation of pulling out the inoculation stick simpler and more easy.
Drawings
FIG. 1 shows a method for producing Morchella strains in example 1 of the present application.
FIG. 2 is a schematic diagram showing the structures of the culture vessel and the inoculation rod used in step S3.
Fig. 3 is a partial enlarged view of the portion a in fig. 2.
Reference numerals illustrate:
1. a culture vessel; 11. sealing cover; 111. air holes; 112. a clamping part; 113. a clamping groove; 12. a bottle body; 13. a breathable and bacteria-isolating membrane; 2. inoculating the rod.
Detailed Description
The technical solutions of the present application will be clearly and completely described below with reference to fig. 1 to 3.
Example 1
The embodiment of the application discloses a preparation method of Morchella strains.
Referring to fig. 1, a method for preparing Morchella strains includes the following six steps:
s1, preparing: the weight ratio of the raw materials is as follows: 50% of wet wheat grains, 37% of wet rice husks, 10% of wet wood dust, 2% of quicklime and 1% of gypsum powder.
Preparing raw materials required by preparing a culture medium according to a proportion, and soaking and rehydrating dry wheat grains, dry wood chips and rice hulls according to a rehydration ratio of 1:1.5.
S2, mixing: the raw materials are uniformly mixed to obtain a culture medium.
S3, charging: providing a culture container and an inoculating rod, filling a part of culture medium into the culture container, stopping filling when the top surface of the culture medium is 3-6 cm away from the mouth of the culture container, then inserting the inoculating rod into the culture container, filling the rest culture medium into the culture container, and sealing the culture container. Note that the weight of the medium per bottle is 400g to 1kg, which enables more sufficient use of the medium.
In the embodiment, the selected culture container is a culture bottle, and the weight of each bottle of culture medium is 600g; in other embodiments, the culture container can also be a culture bag, and especially when the culture bag is used for preparing cultivars, the culture bag is more convenient to use, and hyphae can be directly taken out by cutting the culture bag.
Referring to fig. 2 and 3, the culture container 1 comprises a threaded connection sealing cover 11 and a bottle body 12, a circular air hole 111 is formed in the center of the sealing cover 11, the inoculating rod 2 is in a thin-wall tubular shape, and the shape and the size of the pipe mouth are matched with those of the air hole 111. The inside of the sealing cover 11 is provided with a plurality of clamping parts 112 around the air holes 111, the clamping parts 112 are provided with clamping grooves 113 with downward notches, the inoculating rod 2 is in interference fit with the clamping grooves 113, the clamping grooves 113 play a role in positioning, and the inoculating rod 2 is kept to be vertically and stably positioned in the center of the culture container 1, so that the shape of the strip-shaped holes is regular, and the position is fixed. In step S3, when the culture vessel 1 is sealed by the sealing cover 11, the inoculating rod 2 is inserted and fixed to the locking part 112 of the sealing cover 11 and faces the air hole 111.
Referring to fig. 2, the sealing cover 11 is detachably connected with a breathable bacteria-isolating film 13, an elastic sealing strip is arranged at the edge of the breathable bacteria-isolating film 13 and is extruded and deformed by the inner wall of the air hole 111, so that the breathable bacteria-isolating film 13 is fixed, and the air hole 111 is blocked. The breathable and bacteria-isolating membrane 13 is made of PTFE high polymer, can breathe and release pressure, has the function of bacteria isolation by the pore diameter of 0.2-0.3 um, makes microorganisms such as external bacteria difficult to enter the culture container 1, and simultaneously can make the oxygen supply amount in the culture container 1 enough.
The specific operation principle of the inoculating rod 2 is as follows: the inoculating rod 2 is inserted into the bottle body 12, the bottom end of the inoculating rod 2 is abutted against the top surface of the culture medium, the sealing cover 11 is covered, the pipe wall of the inoculating rod 2 is aligned with the clamping groove 113, the sealing cover 11 is closed in a rotating mode, the inoculating rod 2 is pressed downwards by the sealing cover 11, and the inoculating rod 2 is fixedly spliced with the sealing cover 11; on the contrary, the sealing cover 11 is opened in a rotating way, and the inoculating rod 2 is pulled out along with the rotation of the sealing cover 11. .
S4, sterilization: the culture vessel containing the medium was sterilized at 123℃for 4 hours and cooled.
The inoculating rod can provide support for the culture medium after the step S3 is filled, and the bar shape Kong Takong during the step S4 sterilization is prevented, so that the integrity of the bar shape holes during the inoculation is ensured.
S5, inoculation: the inoculating rod leaves a vertical bar-shaped hole in the culture medium, and the strain of the previous stage is placed in the bar-shaped hole and fills the bar-shaped hole in a ten-thousand-level aseptic chamber or a purifying inoculating assembly line. In this example, the strip hole depth was 5cm.
Compared with the traditional inoculation mode, the method has the advantages that the strip-shaped holes are reserved in the culture medium, the strip-shaped holes are filled with the strain at the upper stage, the growth path of the mycelia covered with the whole culture container can be shortened while the density of the strain is improved, the mycelia can grow the whole culture container faster, the culture period of the mycelia growth is shortened, the development of the mixed bacteria is restrained in time and space, and the probability of infection of the mixed bacteria can be reduced.
When the inoculation in the step S5 is carried out, the strain of the previous stage can be directly put into the culture container from the orifice of the inoculation rod; the inoculating rod is pulled out after the inoculation is completed. The sealing cover is in interference fit with the inoculating rod, so that the inoculating rod can be pulled out synchronously when the sealing cover is opened, the operation of pulling out the inoculating rod is simpler, and the defects that the surface of the inoculating rod is smooth and straight and inconvenient to take out are overcome. Finally, the inoculating rod is pulled out to simplify the operation flow.
Both stock and cultivar can be produced by the above method. When the stock seed is manufactured, the strain of the previous stage is a test tube seed; when the cultivated species is produced, the strain of the previous stage is used as the stock seed.
S6, culturing: culturing strain in a hundred thousand-level clean room, and culturing at 12deg.C for 25 days until the culture container is full of mycelia for inoculation or sowing at the next stage.
Example 2
Example 2 differs from example 1 in the temperature environment and time of the sterilization at step S4 and the incubation at step S6.
S4, sterilization: sterilizing the culture container filled with the culture medium for 6 hours at the temperature of 126 ℃ and cooling;
s6, culturing: culturing strain in a hundred thousand-level clean room for 40 days at 20deg.C, and inoculating or seeding the culture container to obtain mycelia.
Example 3
Example 3 differs from examples 1 and 2 in the temperature environment and time of the sterilization at step S4 and the incubation at step S6.
S4, sterilization: sterilizing the culture container filled with the culture medium for 5 hours at 125 ℃ and cooling;
s6, culturing: culturing strain in hundred thousand clean room at 16deg.C for 35 days, and culturing until the container is full of mycelia for inoculation or sowing at the next stage.
Example 4
Example 4 differs from example 1 in the ratio of the raw materials of the medium.
S1, preparing: the weight ratio of the raw materials is as follows: 60% of wet wheat grains, 27% of wet rice husks, 10% of wet wood dust, 2% of quicklime and 1% of gypsum powder.
The foregoing is illustrative of the present application, and it will be apparent that the foregoing embodiments are merely illustrative of the present application and are not intended to limit the scope of the application in any way, therefore. Equivalent changes according to the structure, shape and principle of the present application should be covered by the protection scope of the present application without making any creative effort.
Claims (7)
1. The preparation method of Morchella strain is characterized by comprising the following steps:
s1, preparing: preparing raw materials required by a culture medium, and soaking and rehydrating the dried plant raw materials, wherein the rehydration rate is 1:1.5;
s2, mixing: mixing the raw materials prepared in the step S1 according to a proportion to obtain a culture medium;
s3, charging: providing a culture container (1) and an inoculating rod (2), filling a part of the culture medium into the culture container, stopping filling when the top surface of the culture medium is 3-6 cm away from the mouth of the culture container, inserting the inoculating rod into the culture container, filling the rest culture medium into the culture container, and sealing the culture container;
s4, sterilization: sterilizing the culture container filled with the culture medium for 4-6 hours at the temperature of 123-126 ℃ and cooling;
s5, inoculation: in a ten-thousand-level aseptic chamber or a purifying inoculation assembly line, a strip-shaped hole is formed in the center of a culture medium after the inoculation rod is pulled out, and a strain of the previous stage is placed in the strip-shaped hole and fills the strip-shaped hole;
s6, culturing: culturing strain in a hundred thousand-level clean room, and culturing at 12-20deg.C for 25-40 days until the culture container is full of mycelia for inoculation or sowing at the next stage.
2. The method for preparing Morchella strain according to claim 1, wherein the mouth of the culture container (1) is provided with a sealing cover (11), the sealing cover (11) is provided with air holes (111), the inoculating rod (2) has a thin-wall tubular structure, the sealing cover (11) can be detachably connected with the inoculating rod (2), and in step S3, when the sealing cover (11) is used for sealing the culture container (1), the inoculating rod (2) is opposite to the air holes (111) and is fixedly connected with the sealing cover (11); when inoculation in the step S5 is carried out, the strain of the previous stage is directly put into the culture container (1) from the pipe orifice of the inoculation rod (2); after the inoculation is completed, the inoculating rod (2) is pulled out.
3. The Morchella strain making method according to claim 2, wherein the sealing cover (11) is detachably connected with a breathable bacteria-isolating film (13), and the breathable bacteria-isolating film (13) is used for sealing the air holes (111).
4. The Morchella strain making method according to claim 3, wherein a plurality of clamping parts (112) are arranged around the air holes (111) on the inner side of the sealing cover (11), a clamping groove (113) with a downward notch is arranged on the clamping part (112), and the inoculating rod (2) is in interference fit with the clamping groove (113).
5. The method for preparing Morchella strains according to claim 1, wherein in the steps S1 and S2, the raw materials of the culture medium are as follows by weight: 50-60% of wet wheat grains, 27-37% of wet rice husks, 10% of wet wood dust, 2% of quicklime and 1% of gypsum powder.
6. The method for preparing Morchella strain according to claim 1, wherein in the step S3, the weight of each culture medium is 400g-1kg.
7. The method for preparing Morchella strain according to claim 1, wherein the strain of the previous stage is a test tube strain; when the cultivated species is produced, the strain of the previous stage is the stock species.
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| JPH1132574A (en) * | 1997-07-25 | 1999-02-09 | Kansai Sogo Kankyo Center:Kk | Tool for forming inoculation hole of culture medium for cultivating mushroom |
| JP3170940U (en) * | 2011-07-27 | 2011-10-06 | 株式会社サカト産業 | Cocoon cultivation container |
| CN103178140A (en) * | 2011-12-20 | 2013-06-26 | 常熟市福莱德连接器科技有限公司 | Photovoltaic panel junction box device |
| CN203446254U (en) * | 2013-04-03 | 2014-02-26 | 桑德环境资源股份有限公司 | Inoculating and spawn running device for cultivating edible fungi with kitchen garbage |
| CN203233729U (en) * | 2013-04-26 | 2013-10-16 | 兴化市恒兴企业管理咨询有限公司 | Inoculating stick for cultivating edible fungi |
| CN104488544A (en) * | 2014-12-03 | 2015-04-08 | 李拴英 | Cultivation method of auriailaria polytricha |
| CN204874522U (en) * | 2015-07-17 | 2015-12-16 | 杭州秀川科技有限公司 | Thallus culture tube |
| CN208443094U (en) * | 2018-06-20 | 2019-01-29 | 合肥美的电冰箱有限公司 | Rack assembly for refrigerator and the refrigerator with it |
| TWI682709B (en) * | 2018-07-02 | 2020-01-21 | 林俊義 | Mushroom cultivation method of mushroom cultivation unit with inoculation hole holder |
| KR20200021723A (en) * | 2018-08-21 | 2020-03-02 | 상주시 | Manufacturing process of Lentinus edodes culture |
| CN210351324U (en) * | 2019-08-21 | 2020-04-17 | 韶关市零壹信息服务有限公司 | Rain-proof water surveillance camera head of intelligence |
| CN113455291A (en) * | 2021-06-30 | 2021-10-01 | 淅川县物华生物科技有限公司 | Fungus stick inoculation device and fungus stick |
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