CN117126717A - Intelligent sampling equipment and sampling method for plankton monitoring in seaweed bed ecosystem - Google Patents
Intelligent sampling equipment and sampling method for plankton monitoring in seaweed bed ecosystem Download PDFInfo
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- 238000005070 sampling Methods 0.000 title claims abstract description 74
- 241001474374 Blennius Species 0.000 title claims abstract description 25
- 238000012544 monitoring process Methods 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 80
- 239000012528 membrane Substances 0.000 claims description 52
- 238000009434 installation Methods 0.000 claims description 43
- 238000005507 spraying Methods 0.000 claims description 14
- 238000004891 communication Methods 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 9
- 239000007921 spray Substances 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000741 silica gel Substances 0.000 claims description 6
- 229910002027 silica gel Inorganic materials 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000206602 Eukaryota Species 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
- 239000006261 foam material Substances 0.000 claims description 3
- 230000005484 gravity Effects 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 230000008054 signal transmission Effects 0.000 claims description 3
- 238000004804 winding Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 12
- 229910052799 carbon Inorganic materials 0.000 description 12
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 239000010410 layer Substances 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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Abstract
An intelligent sampling device and a sampling method for plankton monitoring in a seaweed bed ecosystem belong to the technical field of water body environment sampling devices. The sampling device comprises a sampling main body, an intelligent acquisition component, a driving component and an intelligent control terminal; the device of the invention is convenient for adjusting the collection depth of the collection tray, can carry out gradient collection on different water body suspended matters, and then adopts the DNA of the water body suspended matters enriched in the seaweed bed, so that the collected samples are more complete, and the detected data are more accurate.
Description
Technical Field
The invention belongs to the technical field of water environment sampling equipment, and particularly relates to intelligent plankton monitoring sampling equipment and a sampling method in a seaweed bed ecosystem.
Background
In recent years, the influence of global climate change is increasingly apparent, and as the largest 'carbon pool' on the earth, the ocean plays a fundamental role in global climate control. The effect and significance of the marine carbon sink (blue carbon) are more remarkable.
Blue carbon refers to the process, activity and mechanism of absorbing carbon dioxide in the atmosphere by using marine organisms and fixing the carbon dioxide in the ocean, and has the characteristics of large carbon fixation amount, high efficiency, long storage time and the like compared with other carbon sinks such as green carbon sink and the like. The storage period of carbon sink in land ecological systems such as forests, grasslands and the like is only decades, and blue carbon sink can be hundreds of years or even thousands of years, so that the carbon sink effect is remarkable. Therefore, blue carbon can effectively slow down greenhouse gas emissions, and has become a leading field of global climate control.
The seaweed bed is one of three coastal blue carbon ecosystems, is a habitat and an important food chain of marine organisms, and has the function of stabilizing offshore substrates and coastlines. The seaweed bed is distributed with abundant microbial communities, plays an important role in the offshore material circulation and the bio-geochemistry process, is important for maintaining the stability of an ecological system, can be used as an early index for effectively identifying the ecological condition of the seaweed, is used for comprehensively and timely grasping the environmental pressure faced by the ecological system of the seaweed, and has important significance for biological monitoring in the seaweed bed.
All water body suspended matters in the water body are uniformly enriched by the biological monitoring intelligent sampling equipment in the existing seaweed bed ecological system, the function of grading step collection is not achieved, and the difficulty of a centrifugal separation process is increased.
Disclosure of Invention
Aiming at the problems, the invention provides intelligent plankton monitoring sampling equipment and a sampling method in a seaweed bed ecological system.
The technical scheme of the invention is as follows: the intelligent sampling equipment for plankton monitoring in the seaweed bed ecosystem comprises a sampling main body, wherein an installation adjusting port communicated with the outside, an intelligent acquisition component arranged at the installation adjusting port, a driving component arranged at the upper end of the sampling main body and connected with the intelligent acquisition component, and an intelligent control terminal are arranged at the center of the sampling main body;
the intelligent acquisition assembly comprises an acquisition main box, a first filter membrane, a second filter membrane, an I-shaped acquisition head and a water suction pump, wherein the acquisition main box is provided with an acquisition sub-box at the left side and the right side of the bottom end, the first filter membrane is arranged in the acquisition main box, the second filter membrane is arranged in the acquisition sub-box, the I-shaped acquisition head is connected with the I-shaped acquisition head, the I-shaped acquisition head comprises a vertical acquisition pipe, a spray disc and an acquisition disc, the upper end of the vertical acquisition pipe is penetrated through the bottom end of the acquisition main box and extends to the inside of the vertical acquisition pipe, the spray disc is arranged in the acquisition main box and is communicated with the upper end of the vertical acquisition pipe, the lower end of the vertical acquisition pipe is connected with the spray disc, a plurality of spray ports are uniformly formed in the bottom end of the acquisition disc, a water flow sensor is arranged at the vertical acquisition pipe, the height of the acquisition sub-box is also adjustable according to requirements, and the length of the I-shaped acquisition head is used for detecting water inflow entering the I-shaped acquisition head;
the first filter membrane and the second filter membrane are of detachable structures, the first filter membrane comprises an installation inner ring sleeved on the outer wall of the vertical collecting tube, an installation outer ring coaxially arranged with the installation inner ring, a plurality of separation rods arranged between the installation inner ring and the installation outer ring, and sub-filter membranes movably arranged between two adjacent separation rods, and the second filter membrane comprises a silica gel membrane and an outer frame for fixing the silica gel membrane;
the driving assembly comprises an H-shaped mounting frame arranged at the upper end of the sampling main body, a rotating roller arranged at the horizontal section of the H-shaped mounting frame, and a driving motor for driving the rotating roller to rotate, wherein a rope for connecting the main collection box is wound on the outer wall of the rotating roller;
the intelligent control terminal comprises a controller electrically connected with the water suction pump and the water flow sensor, a GPS module arranged on the sampling main body, a wireless communication module arranged on the sampling main body and used for remote signal transmission, and a fault alarm module.
According to one aspect of the invention, the bottom end of the sampling main body is provided with the anti-tipping component, the anti-tipping component comprises an installation sliding plate vertically arranged at the bottom end of the sampling main body, an inverted T-shaped fixed column movably connected with the center of the installation sliding plate, balance sliding plates symmetrically distributed on the left side and the right side of the inverted T-shaped fixed column, the upper end of the balance sliding plate is in sliding connection with the installation sliding plate, and sliding sleeves sleeved outside the vertical section of the inverted T-shaped fixed column, the outer wall of the sliding sleeves is uniformly provided with a plurality of blades along the circumferential direction, a plurality of rebound springs are uniformly arranged between the sliding sleeves and the horizontal section of the inverted T-shaped fixed column, and an adjusting sliding rod is hinged between the sliding sleeves and each balance sliding plate.
According to one aspect of the invention, the left side and the right side of the installation slide plate are respectively provided with a limited-steric spacer, the balance slide plate comprises a sliding plate which is in sliding connection with the installation slide plate and a balance plate which is arranged below the sliding plate and has a length longer than that of the sliding plate, the balance plate is of a hollow structure, foam materials are filled in the balance plate of the hollow structure, the balance plate slides left and right on the installation slide plate through the sliding plate so as to adjust the distance of the balance plate, and the separation between the sliding plate and the installation slide plate can be avoided through the arrangement of the limited-steric spacer, so that the normal use of the anti-tipping assembly is influenced.
According to one aspect of the invention, the bottom end of the collecting tray is provided with the sleeve, the bottom end of the sleeve is provided with the electric crushing roller, a filter screen is arranged in the sleeve and positioned at the upper end of the electric crushing roller, plants in the water body are crushed by the electric crushing roller, fine solid impurities in the water body are filtered by the filter screen, and the solid impurities and plants in the water body are prevented from entering the collecting tray to cause blockage, so that the normal operation of the collecting work is influenced.
According to one aspect of the invention, the side wall of the sampling main body is provided with the anti-collision protection assembly, the anti-collision protection assembly comprises a horizontal arc-shaped buffer plate connected with the side wall of the sampling main body through a plurality of first spring connecting columns and a plurality of vertical arc-shaped buffer plates connected with the horizontal arc-shaped buffer plate through a second spring connecting column, and compared with a buffer plate with a single-layer structure, the anti-collision protection assembly has the advantages that a double-layer buffer structure is formed through the horizontal arc-shaped buffer plate and the vertical arc-shaped buffer plate, the anti-collision protection assembly has better buffer capacity, and the service life of the buffer device is prolonged.
According to one aspect of the invention, each side wall of the vertical arc-shaped buffer plate is provided with a buffer air bag, so that the vertical arc-shaped buffer plate is prevented from being damaged due to direct impact of external force on the vertical arc-shaped buffer plate.
According to one aspect of the invention, the pore size of the first filter membrane is 0.45 mu m, the pore size of the second filter membrane is 0.2 mu m, eukaryotes in the water body are enriched through the first filter membrane, bacteria in the water body are enriched through the second filter membrane, and then the enriched water body suspended solid DNA is adopted, so that the collected samples are more complete, and the detected data are more accurate.
The invention also discloses an intelligent sampling method for monitoring plankton in the seaweed bed ecological system, which is based on the intelligent sampling device for monitoring plankton in the seaweed bed ecological system and comprises the following steps:
s1, controlling an external driving device to move a sampling main body to a specified water environment area through a controller, starting a driving motor, driving a rotating roller to rotate clockwise through the driving motor, and continuously winding a rope down from the rotating roller, wherein at the moment, under the action of gravity of a main collecting box, collecting sub boxes at two sides of the main collecting box slide downwards in an installation adjusting port, a collecting disc synchronously moves downwards into a water body, and when the collecting disc reaches a preset collecting depth, controlling the external driving device to be closed through the controller;
s2, starting a water suction pump, enabling water to flow into the collecting disc through each collecting hole, enabling the water to flow into the spraying disc through the vertical collecting tube, enabling the water to be uniformly sprayed onto the first filter membrane through the spraying opening, enriching eukaryotes in the water environment through the first filter membrane, enabling the water to flow down through the first filter membrane, enabling the water to respectively fall into the collecting sub-boxes from two sides of the inverted T-shaped fixing column, enriching bacteria in the water environment through the second filter membrane, enabling the water to flow into the water environment through the second filter membrane, detecting the water quantity entering the inverted T-shaped fixing column through the water flow sensor, and completing collection of microorganism DNA in the seaweed bed when the preset water quantity is reached;
s3, during sample collection, plants in the water body are crushed through an electric crushing roller, fine solid impurities in the water body are filtered through a filter screen, a double-layer buffer structure is formed through a horizontal arc buffer plate and a vertical arc buffer plate, and the impact of external force on a sampling main body is prevented;
s4, when a sample is collected, wind flow can vertically blow to each blade, so that the sliding sleeve is driven to move towards the side close to the mounting sliding plate, at the moment, the sliding sleeve compresses each rebound spring, and the corresponding balance sliding plate is pushed by the adjusting sliding rod to slide along the left side and the right side of the mounting sliding plate respectively, so that the balance sliding plates are positioned on the left side and the right side of the sampling main body respectively, and the sampling main body is kept balanced.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention provides plankton monitoring intelligent sampling equipment and a sampling method in a seaweed bed ecological system, which are convenient for adjusting the collection depth of a collection disc and can carry out gradient collection on different water body suspended matters by arranging the collection main box which is in sliding connection with the inside of a mounting and adjusting port, and then adopt the DNA of the water body suspended matters enriched in the seaweed bed ecological system, so that the collected samples are more complete, and the detected data is more accurate;
(2) The invention also provides an anti-tipping assembly, when a sample is collected and encounters a large wind speed, the balance sliding plates respectively slide to the left side and the right side of the sampling main body, so that the purpose of balancing the sampling main body is achieved, the sampling main body is prevented from tipping, the running stability of the sampling main body is improved, an external driving element is not needed in the whole process, the device has the advantages of green and energy saving, and when the device is not used, the two balance sliding plates are mutually close and are stored at the bottom end of the sampling main body, so that the placing space can be saved;
(3) Compared with the existing single-layer buffer structure, the anti-collision protection assembly is of a double-layer buffer structure formed by the horizontal arc-shaped buffer plate and the vertical arc-shaped buffer plate, has better buffer capacity, and meanwhile, the side wall of the vertical arc-shaped buffer plate is also provided with the buffer air bag, so that the vertical arc-shaped buffer plate is prevented from being directly impacted by external force, the vertical arc-shaped buffer plate is prevented from being damaged, and the service life of the anti-collision protection assembly is greatly prolonged.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
FIG. 3 is a schematic view of the mounting structure of the anti-rollover assembly of the present invention on a sampling body;
FIG. 4 is a bottom view of the anti-rollover assembly of the present invention;
FIG. 5 is a schematic view of the structure of a first filter membrane of the present invention;
the device comprises a 1-sampling main body, a 10-installation adjusting port, a 11-tipping prevention assembly, a 110-installation sliding plate, a 111-inverted T-shaped fixed column, a 112-balance sliding plate, a 1120-sliding plate, a 1121-balance plate, a 113-sliding sleeve, 1130-blades, 1131-rebound springs, 1132-adjustment sliding rods, 114-limit blocking blocks, a 12-anti-collision protection assembly, a 120-horizontal arc buffer plate, a 1200-first spring connecting column, a 121-vertical arc buffer plate, a 1210-second spring connecting column, 1211-buffer air bags, a 2-intelligent acquisition assembly, a 20-acquisition main box, a 200-acquisition sub-box, a 21-first filter membrane, a 210-installation inner ring, a 211-installation outer ring, a 212-separation rod, a 213-sub-filter membrane, a 22-second filter membrane, a 220-outer frame, a 23-I-shaped acquisition head, a 230-vertical acquisition tube, a 231-spraying disc, a 232-acquisition disc, a 233-spraying port, a 234-acquisition hole, a 235-water flow sensor, a 236-sleeve, a 2360-electric crushing roller, a 2361-water pump, a 24-filter screen, a 3-H-driving assembly, a 3-filter screen, a 31-driving rope, a communication module, a 43-communication module, a 43-and a communication control module, a communication module, a fault control module, a 43-and a communication module.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1
As shown in fig. 1 and 2, an intelligent sampling device for monitoring plankton in a seaweed bed ecosystem comprises a sampling main body 1, wherein an installation and adjustment port 10 communicated with the outside, an intelligent acquisition component 2 arranged at the installation and adjustment port 10, a driving component 3 arranged at the upper end of the sampling main body 1 and connected with the intelligent acquisition component 2, and an intelligent control terminal 4 are arranged at the center of the sampling main body 1;
as shown in fig. 5, the intelligent collecting assembly 2 comprises a collecting main box 20 with collecting sub-boxes 200 at the left and right sides of the bottom end, a first filter membrane 21 arranged in the collecting main box 20, a second filter membrane 22 arranged in the collecting sub-boxes 200, an i-shaped collecting head 23 and a water suction pump 24 connected with the i-shaped collecting head 23, wherein the i-shaped collecting head 23 comprises a vertical collecting pipe 230, a spraying disk 231, a collecting disk 232 and 10 collecting holes 234, the upper end of the vertical collecting pipe 230 penetrates through the bottom end of the collecting main box 20 and extends to the inside of the vertical collecting pipe 230, the spraying disk 231 is arranged in the collecting main box 20 and is communicated with the upper end of the vertical collecting pipe 230, the collecting disk 232 is connected with the lower end of the vertical collecting pipe 230, 10 spraying ports 233 are uniformly arranged at the bottom end of the spraying disk 231, and a water flow sensor 235 is arranged at the vertical collecting pipe 230;
the first filter membrane 21 and the second filter membrane 22 are both of detachable structures, the first filter membrane 21 comprises an installation inner ring 210 sleeved on the outer wall of a vertical collecting tube 230, an installation outer ring 211 coaxially arranged with the installation inner ring 210, 4 separation rods 212 arranged between the installation inner ring 210 and the installation outer ring 211, and a sub-filter membrane 213 movably arranged between two adjacent separation rods 212, and the second filter membrane 22 comprises a silica gel membrane and an outer frame 220 for fixing the silica gel membrane;
the driving assembly 3 comprises an H-shaped mounting frame 30 arranged at the upper end of the sampling main body 1, a rotating roller 31 arranged at the horizontal section of the H-shaped mounting frame 30, and a driving motor 32 for driving the rotating roller 31 to rotate, wherein a rope 310 for connecting the main collecting box 20 is wound on the outer wall of the rotating roller 31;
the intelligent control terminal 4 comprises a controller 40 electrically connected with the water pump 24 and the water flow sensor 235, a GPS module 41 arranged on the sampling main body 1, a wireless communication module 42 arranged on the sampling main body 1 and used for remote signal transmission and a fault alarm module 43;
a sleeve 236 is arranged at the bottom end of the collecting disc 232, an electric crushing roller 2360 is arranged at the bottom end of the sleeve 236, and a filter screen 2361 is arranged in the sleeve 236 and positioned at the upper end of the electric crushing roller 2360;
the pore size of the first filter 21 was 0.45. Mu.m, and the pore size of the second filter 22 was 0.2. Mu.m.
Example 2
This embodiment differs from embodiment 1 in that:
as shown in fig. 3 and 4, the bottom end of the sampling main body 1 is provided with an anti-tipping component 11, the anti-tipping component 11 comprises an installation slide plate 110 vertically arranged at the bottom end of the sampling main body 1, an inverted-T-shaped fixing column 111 with a vertical section and the center of the installation slide plate 110 being connected through bolts, balance slide plates 112 symmetrically distributed at the left side and the right side of the inverted-T-shaped fixing column 111 and with the upper end being in sliding connection with the installation slide plate 110, and a sliding sleeve 113 sleeved outside the vertical section of the inverted-T-shaped fixing column 111 and provided with 4 blades 1130 uniformly along the circumferential direction, 4 rebound springs 1131 are uniformly arranged between the sliding sleeve 113 and the horizontal section of the inverted-T-shaped fixing column 111, and an adjusting sliding rod 1132 is hinged between the sliding sleeve 113 and each balance slide plate 112;
the left side and the right side of the installation slide plate 110 are respectively provided with a limiting spacer 114, the balance slide plate 112 comprises a slide plate 1120 which is in sliding connection with the installation slide plate 110, and a balance plate 1121 which is arranged below the slide plate 1120 and has a length longer than that of the slide plate 1120, the balance plate 1121 is of a hollow structure, and foam materials are filled in the balance plate 1121 of the hollow structure.
Example 3
This embodiment differs from embodiment 2 in that:
as shown in fig. 1 and 2, the side wall of the sampling body 1 is provided with an anti-collision protection assembly 12, and the anti-collision protection assembly 12 comprises a horizontal arc-shaped buffer plate 120 connected with the side wall of the sampling body 1 through a plurality of first spring connecting columns 1200 and a plurality of vertical arc-shaped buffer plates 121 connected with the horizontal arc-shaped buffer plate 120 through a plurality of second spring connecting columns 1210;
each of the vertical arc-shaped buffer plates 121 is provided with a buffer air bag 1211 at a sidewall thereof.
The water flow sensor 235, the electric crushing roller 2360, the air pump 152, the water pump 24, the rotating roller 31, the driving motor 32, the controller 40, the GPS module 41, the wireless communication module 42 and the failure alarm module 43 used in this embodiment are all made of the products of the prior art, and can be selected by those skilled in the art according to the needs, so that the technical scheme of the present invention can be satisfied, and no special limitation is made herein.
Example 4
The embodiment discloses an intelligent sampling method for monitoring plankton in a seaweed bed ecosystem, which is based on intelligent sampling equipment for monitoring plankton in the seaweed bed ecosystem in embodiments 1-3, and comprises the following steps:
s1, controlling an engine to move a sampling main body 1 to a specified water environment area through a controller 40, starting a driving motor 32, driving a rotating roller 31 to rotate clockwise through the driving motor 32, enabling a winding rope 310 to continuously wind down from the rotating roller 31, at the moment, under the action of gravity of a main collection box 20, collecting sub-boxes 200 on two sides of the main collection box slide downwards in an installation adjusting port 10, a collection disc 232 synchronously moves downwards into a water body, and controlling an external driving device to be closed through the controller 40 when the collection disc 232 reaches a preset collection depth;
s2, starting a water suction pump 24, enabling water to flow through each collecting hole 234 to enter a collecting tray 232, enabling the water to enter a spraying tray 231 through a vertical collecting tube 230, uniformly spraying the water onto a first filter membrane 21 through a spraying opening 233, enriching eukaryotes in water environment through the first filter membrane 21, enabling the water to flow down through the first filter membrane 21, respectively falling into a collecting sub-box 200 from two sides of an inverted T-shaped fixing column 111, enriching bacteria in the water environment through a second filter membrane 22, enabling the water to flow into the water environment through the second filter membrane 22, detecting the water quantity entering the inverted T-shaped fixing column 111 through a water flow sensor 235, and completing collection of microorganism DNA in a seaweed bed when the preset water quantity is reached;
s3, during sample collection, plants in the water body are crushed through an electric crushing roller 2360, fine solid impurities in the water body are filtered through a filter screen 2361, a double-layer buffer structure is formed through a horizontal arc buffer plate 120 and a vertical arc buffer plate 121, and the sampling main body 1 is prevented from being collided by external force;
s4, when a sample is collected, wind flow can vertically blow to each blade 1130, so that the sliding sleeve 113 is driven to move towards the side close to the mounting sliding plate 110, at the moment, the sliding sleeve 113 compresses each rebound spring 1131, and the corresponding balance sliding plate 112 is pushed by the adjusting sliding rod 1132 to slide along the left side and the right side of the mounting sliding plate 110 respectively, so that the balance sliding plates 112 are positioned on the left side and the right side of the sampling main body 1 respectively, and the sampling main body 1 is balanced.
Claims (7)
1. The intelligent sampling equipment for plankton monitoring in the seaweed bed ecological system is characterized by comprising a sampling main body (1), wherein an installation adjusting port (10) communicated with the outside, an intelligent acquisition component (2) arranged at the installation adjusting port (10), a driving component (3) arranged at the upper end of the sampling main body (1) and connected with the intelligent acquisition component (2) and an intelligent control terminal (4) are arranged at the center of the sampling main body (1);
the intelligent acquisition assembly (2) comprises an acquisition main box (20) with acquisition sub-boxes (200) arranged at the left side and the right side of the bottom end, a first filter membrane (21) arranged in the acquisition main box (20), a second filter membrane (22) arranged in the acquisition sub-boxes (200), an I-shaped acquisition head (23) and a water suction pump (24) connected with the I-shaped acquisition head (23), wherein the I-shaped acquisition head (23) comprises a vertical acquisition pipe (230) with the upper end penetrating through the bottom end of the acquisition main box (20) and extending to the inside, a spray disc (231) arranged in the acquisition main box (20) and communicated with the upper end of the vertical acquisition pipe (230) and an acquisition disc (232) connected with the lower end of the vertical acquisition pipe (230), a plurality of spray ports (233) are uniformly arranged at the bottom end of the spray disc (231), a plurality of acquisition holes (234) are uniformly arranged at the bottom end of the acquisition disc (232), and a water flow sensor (235) is arranged at the vertical acquisition pipe (230);
the first filter membrane (21) and the second filter membrane (22) are of detachable structures, the first filter membrane (21) comprises an installation inner ring (210) sleeved on the outer wall of the vertical collecting tube (230), an installation outer ring (211) coaxially arranged with the installation inner ring (210), a plurality of separation rods (212) arranged between the installation inner ring (210) and the installation outer ring (211) and sub-filter membranes (213) movably arranged between two adjacent separation rods (212), and the second filter membrane (22) comprises a silica gel membrane and an outer frame (220) for fixing the silica gel membrane;
the driving assembly (3) comprises an H-shaped mounting frame (30) arranged at the upper end of the sampling main body (1), a rotating roller (31) arranged at the horizontal section of the H-shaped mounting frame (30), and a driving motor (32) for driving the rotating roller (31) to rotate, wherein a rope (310) for connecting the main collection box (20) is wound on the outer wall of the rotating roller (31);
the intelligent control terminal (4) comprises a controller (40) electrically connected with the water suction pump (24) and the water flow sensor (235), a GPS module (41) arranged on the sampling main body (1), a wireless communication module (42) arranged on the sampling main body (1) and used for remote signal transmission, and a fault alarm module (43).
2. The device of claim 1, wherein the bottom end of the sampling main body (1) is provided with an anti-tipping component (11), the anti-tipping component (11) comprises an installation sliding plate (110) vertically arranged at the bottom end of the sampling main body (1), an inverted-T-shaped fixing column (111) movably connected with the center of the installation sliding plate (110), balance sliding plates (112) symmetrically distributed on the left side and the right side of the inverted-T-shaped fixing column (111) and slidably connected with the installation sliding plate (110) at the upper end, and sliding sleeves (113) sleeved outside the vertical section of the inverted-T-shaped fixing column (111) and provided with a plurality of blades (1130) on the outer wall uniformly along the circumferential direction, a plurality of rebound springs (1131) are uniformly arranged between the sliding sleeves (113) and the horizontal section of the inverted-T-shaped fixing column (111), and an adjusting sliding rod (1132) is hinged between each of the sliding sleeves (113) and each balance sliding plate (112).
3. The device according to claim 2, wherein the mounting slide plate (110) is provided with limited-steric spacer blocks (114) on both left and right sides, the balancing slide plate (112) comprises a sliding plate (1120) slidably connected with the mounting slide plate (110), and a balancing plate (1121) arranged below the sliding plate (1120) and having a length greater than that of the sliding plate (1120), the balancing plate (1121) is of a hollow structure, and foam materials are filled in the balancing plate (1121) of the hollow structure.
4. The device according to claim 1, characterized in that the collecting tray (232) is provided with a sleeve (236) at the bottom end, the sleeve (236) is provided with an electric crushing roller (2360) at the bottom end, and a filter screen (2361) is provided in the sleeve (236) and at the upper end of the electric crushing roller (2360).
5. The apparatus of claim 1, wherein the sampling body (1) sidewall is provided with a bump protection assembly (12), the bump protection assembly (12) comprising a horizontal arc-shaped buffer plate (120) connected to the sampling body (1) sidewall by a plurality of first spring connection posts (1200), a plurality of vertical arc-shaped buffer plates (121) connected to the horizontal arc-shaped buffer plate (120) by a second spring connection post (1210).
6. The apparatus of claim 5, wherein each of said vertical arcuate buffer plate (121) sidewalls is provided with a buffer bladder (1211).
7. An intelligent sampling method for monitoring plankton in a seaweed bed ecosystem based on the device of any one of claims 1-6, characterized by comprising the following steps:
s1, controlling an external driving device to move a sampling main body (1) to a specified water environment area through a controller (40), starting a driving motor (32), driving a rotating roller (31) to rotate clockwise through the driving motor (32), enabling a winding rope (310) to continuously wind down from the rotating roller (31), at the moment, under the action of gravity of a main collecting box (20), collecting sub-boxes (200) on two sides of the main collecting box downwards slide in an installation adjusting port (10), and a collecting disc (232) synchronously downwards moves into a water body, and controlling the external driving device to be closed through the controller (40) when the collecting disc (232) reaches a preset collecting depth;
s2, starting a water suction pump (24), enabling water to flow through each collecting hole (234) to enter a collecting disc (232), enabling the water to enter a spraying disc (231) through a vertical collecting tube (230), evenly spraying the water onto a first filter membrane (21) through a spraying opening (233), enriching eukaryotes in water environment through the first filter membrane (21), enabling water flow to flow down through the first filter membrane (21), respectively falling into a collecting sub-box (200) from two sides of an inverted T-shaped fixed column (111), enriching bacteria in the water environment through a second filter membrane (22), enabling the water flow to flow into the water environment through the second filter membrane (22), detecting the water quantity entering the inverted T-shaped fixed column (111) through a water flow sensor (235), and completing collection of microorganism DNA in a seaweed bed when the preset water quantity is reached;
s3, during sample collection, plants in the water body are crushed through an electric crushing roller (2360), fine solid impurities in the water body are filtered through a filter screen (2361), and a double-layer buffer structure is formed through a horizontal arc buffer plate (120) and a vertical arc buffer plate (121), so that the impact of external force on the sampling main body (1) is prevented;
s4, when a sample is collected, wind flow can vertically blow to each blade (1130), so that the sliding sleeve (113) is driven to move towards the side close to the mounting sliding plate (110), at the moment, the sliding sleeve (113) compresses each rebound spring (1131), the corresponding balance sliding plate (112) is pushed by the adjusting sliding rod (1132) to slide along the left side and the right side of the mounting sliding plate (110) respectively, the balance sliding plates (112) are located on the left side and the right side of the sampling main body (1) respectively, and the sampling main body (1) is kept balanced.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN202310604431.8A CN117126717A (en) | 2023-05-26 | 2023-05-26 | Intelligent sampling equipment and sampling method for plankton monitoring in seaweed bed ecosystem |
| JP2023119532A JP7377400B1 (en) | 2023-05-26 | 2023-07-23 | Intelligent monitoring and sampling equipment and sampling method of planktonic organisms in seagrass bed ecosystem |
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| CN202310604431.8A CN117126717A (en) | 2023-05-26 | 2023-05-26 | Intelligent sampling equipment and sampling method for plankton monitoring in seaweed bed ecosystem |
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| CN117126717A true CN117126717A (en) | 2023-11-28 |
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| CN117668472A (en) * | 2024-02-02 | 2024-03-08 | 暨南大学 | Island reef environment multi-parameter monitoring method and system |
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| CN117723349B (en) * | 2023-11-24 | 2024-06-04 | 青岛海洋地质研究所 | Water sample automatic acquisition device for amphibious air-sea aircraft |
| CN117890159A (en) | 2023-12-19 | 2024-04-16 | 广东智环研生态科技发展有限公司 | Integrated sampling and separating device and method for detecting microplastic in water |
| CN117589960B (en) * | 2024-01-18 | 2024-04-16 | 浙江甬信检测技术有限公司 | River water quality parameter comprehensive monitor |
Family Cites Families (9)
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| JP2002286597A (en) | 2001-03-22 | 2002-10-03 | Nichiyu Giken Kogyo Co Ltd | Trigger for detection reach to bottom under submerged condition |
| JP2003189756A (en) | 2001-12-26 | 2003-07-08 | Mitsubishi Heavy Ind Ltd | Method for extracting factor affecting marine ecosystem and method for controlling marine ecosystem |
| JP3607284B2 (en) | 2003-03-31 | 2005-01-05 | 株式会社ミキモト | Method for detecting harmful water quality environment and water quality environment monitoring system |
| JP5041721B2 (en) | 2006-03-29 | 2012-10-03 | 中国電力株式会社 | Plankton distribution research ship |
| CN109162267A (en) | 2018-10-31 | 2019-01-08 | 浙江海洋大学 | A kind of collecting refuse from open water device |
| CN213275012U (en) | 2020-10-19 | 2021-05-25 | 天津绿格瑞科技发展有限公司 | Marine pollution sampling equipment |
| JP7507082B2 (en) | 2020-12-24 | 2024-06-27 | 大成建設株式会社 | Environmental DNA sample collection device and environmental DNA sample collection method |
| CN112964507B (en) | 2021-02-01 | 2021-10-15 | 生态环境部南京环境科学研究所 | Integrated intelligent acquisition and analysis device for collecting and analyzing underground water leaching solution |
| CN114239422B (en) | 2022-02-23 | 2022-05-17 | 中国海洋大学 | Method for improving marine chlorophyll a concentration prediction accuracy based on machine learning |
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| CN117668472A (en) * | 2024-02-02 | 2024-03-08 | 暨南大学 | Island reef environment multi-parameter monitoring method and system |
| CN117668472B (en) * | 2024-02-02 | 2024-04-05 | 暨南大学 | Island reef environment multi-parameter monitoring method and system |
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