CN117106877A - RHD gene primer, primer mixed system amplification method, amplification product quality detection method, sequencing library construction method and sequencing method - Google Patents

RHD gene primer, primer mixed system amplification method, amplification product quality detection method, sequencing library construction method and sequencing method Download PDF

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CN117106877A
CN117106877A CN202310144604.2A CN202310144604A CN117106877A CN 117106877 A CN117106877 A CN 117106877A CN 202310144604 A CN202310144604 A CN 202310144604A CN 117106877 A CN117106877 A CN 117106877A
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primer
nucleotide sequence
sequencing
rhd
rhd gene
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王博
陈超琼
张悦
何润钧
杨晓宏
王硕
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Xi'an Haorui Gene Technology Co ltd
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Abstract

The disclosure relates to the technical field of gene sequencing, and relates to an RHD gene primer, a primer mixed system amplification method, an amplification product quality detection method, a sequencing library construction method and a sequencing method, wherein the primer sequence comprises the following steps: primer 1: RHD1-F, primer 2: RHD1-R, primer 3: RHD2-F, primer 4: RHD2-R, primer 5: RHD3-F, primer 6: RHD3-R, primer 7: RHD4-F, primer 8: RHD4-R, primer 9: RHD5-F, primer 10: RHD5-R, primer 11: RHD6-F, primer 12: RHD6-R, primer 13: RHD7-F, primer 14: RHD7-R, primer 15: RHD-BOX-F, primer 16: RHD-BOX-R; the primer mixture system is prepared based on the primer, the amplification product is obtained after the primer mixture system is amplified, a sequencing library is constructed based on the amplification product, and the HiFiready mode is used for sequencing the RHD gene sequencing library, so that the accuracy of a sequencing result can be effectively improved.

Description

RHD gene primer, primer mixed system amplification method, amplification product quality detection method, sequencing library construction method and sequencing method
Technical Field
The disclosure relates to the technical field of gene sequencing, in particular to a RHD gene primer, a primer mixed system amplification method, an amplification product quality detection method, a sequencing library construction method and a sequencing method.
Background
The Rh blood group system is the most complex and most polymorphic system of genes in the erythrocyte blood group, wherein the D antigen is the most important antigen of the Rh system, because the D antigen is closely related to hemolytic transfusion reaction and fetal and neonatal hemolytic diseases, and has very important effects of accurately distinguishing the genotypes and determining the types of the antigens, and more scientifically guiding transfusion work and further improving experimental diagnosis of the neonatal hemolytic diseases and maintaining health of mother and infant. According to the RHD phenotype, it is classified into the following 5 classes: d-positive, D-negative, DEL, weakD and partialD (variants for short), classical serological tests can distinguish between D-positive and partial D-negative, although more sensitive monoclonal antibodies are currently produced, not all anti-D reagents can detect partial or weak expression of D antigen, so weakD, DEL, partialD is prone to false detection, missed detection, etc. Aiming at DEL type alleles, the D antigen coded by the alleles has very weak expression, large logarithms can be identified only through absorption and diffusion, and the detection effect is not completely accurate and has high cost; some of the weakD, partialD alleles lead to reduced or altered expression of erythrocyte surface D epitopes, and donors and patients with these atypical alleles may be serologically misclassified negative because many of these alleles do not react identically to all anti-D typing agents, resulting in incorrect interpretation of the results, hemolytic transfusion reactions and neonatal hemolysis.
In recent years, with the innovation of technology, genotyping is gradually common, for example, the detection of RHD blood types by adopting a classical serology method is more common in clinic, and the detection of negative and positive results by adopting the method is more accurate, but the judgment of the results by adopting the method is subjective, and is particularly prominent in weak or visual field agglutination reaction, so that the reliability of the results is affected, namely, false detection is easy to occur in detection of partial DEL or variants; for the same antibody or autoantibody individual, a special method is needed to treat the erythrocytes, but the erythrocytes are destroyed to express antigens, the result identification is influenced, and the process is complex and tedious; for patients with multiple transfusions or large numbers of transfusions, the large number of donor erythrocytes in the individual can severely interfere with typing; in addition, many variants of antibodies are expensive to produce and do not perform well. The PCR-SSP method has the advantages of simple operation, low experiment cost, short time effect and easy analysis, but the technology can not detect new alleles and types, the types of the detected alleles are limited, if the detection is too many, the experiment engineering amount is huge, the corresponding labor cost and the reagent cost are also increased exponentially, and the experiment result is also due to the problem of specificity of detection sites, and the design difficulty of the primers is increased; the PCR-SBT also has the advantages of simple operation, low experimental cost, short time effect and easy analysis, can detect new alleles, but also can not be typed, and because the technology is limited, only amplified exons are taken as main flow, the variants generated by the multi-exon combined mutation can not be well distinguished, and an ambiguous result is generated; NGS technology can detect new allelic advantages, but also because of the length of reading, assembly relies heavily on data calculations, and more small fragment assembly introduces a large number of ambiguous results.
It should be noted that the information disclosed in the above background section is only for enhancing understanding of the background of the present disclosure and thus may include information that does not constitute prior art known to those of ordinary skill in the art.
Disclosure of Invention
The purpose of the present disclosure is to provide a primer for RHD gene, a primer mixture system amplification method, an amplification product quality inspection method, a sequencing library construction method and a sequencing method, so as to obtain the full-length sequence (fig. 2) of RHD gene, thereby overcoming the situation that the accuracy of the conventional sequencing method is not high due to the limitations and defects of the related art at least to a certain extent.
Other features and advantages of the present disclosure will be apparent from the following detailed description, or may be learned in part by the practice of the disclosure.
According to a first aspect of the present disclosure there is provided an RHD gene primer, the primer sequence of which comprises:
primer 1: RHD1-F, nucleotide sequence:
CACAACCTGCGAAGCACGGGA；
primer 2: RHD1-R, nucleotide sequence:
CCTCGCCCACAGCCTCTACTCTACC；
primer 3: RHD2-F, nucleotide sequence:
GATGGTGTCAGGGGAGGGAGGTG；
primer 4: RHD2-R, nucleotide sequence:
AACAGAACCAAATCAGTCCCAAGTCAAGT；
primer 5: RHD3-F, nucleotide sequence:
ATTCTGGCAACCTAATGAAGGAGTATGA；
primer 6: RHD3-R, nucleotide sequence:
GCTCCTTGACCGCTCGTCTCTTT；
primer 7: RHD4-F, nucleotide sequence:
AACTCACCAGAATACAGAACTCAGTCTCACA；
Primer 8: RHD4-R, nucleotide sequence:
CTTCAGCCAAAGCAGAGGAGGTTAG；
primer 9: RHD5-F, nucleotide sequence:
TCAGTGTAAGAACCATAAAGGTGTATCTGTGTAGT；
primer 10: RHD5-R, nucleotide sequence:
TAGTTTTCTTATCAGTCGGTAGTTGTGTCA；
primer 11: RHD6-F, nucleotide sequence:
CAGGACTGTTGGGATGCTACTGGATTT；
primer 12: RHD6-R, nucleotide sequence:
CCAATAAAAGATGTTTGCTTGTAGAATAAAGGTGA；
primer 13: RHD7-F, nucleotide sequence:
GTTTTGATAACCTGAACTGTGTTTGGAGC；
primer 14: RHD7-R, nucleotide sequence:
GCAGTCTGTTGTTTACCAAGATGTTGTTATGT；
primer 15: RHD-BOX-F, nucleotide sequence is:
AAACAAATACCTGAAACACCTACACTACAAAAATG；
primer 16: RHD-BOX-R, nucleotide sequence is:
TGTAAGCCGAGCAGTAGAGACCTGAGAT。
alternatively, the RHD gene primers are synthesized based on 5' phosphorylation using primer 1-primer 16.
According to a second aspect of the present disclosure, there is provided a primer mixture system, wherein the RHD gene primer is diluted to a working concentration and formulated according to a predetermined ratio, and the working concentration is: 100uM; the primer mixture system comprises:
0.04. Mu.L of primer 1, 0.04. Mu.L of primer 2, 0.06. Mu.L of primer 3, 0.06. Mu.L of primer 4, 0.05. Mu.L of primer 5, 0.05. Mu.L of primer 6, 0.03. Mu.L of primer 7, 0.03. Mu.L of primer 8, 0.05. Mu.L of primer 9, 0.05. Mu.L of primer 10, 0.05. Mu.L of primer 11, 0.05. Mu.L of primer 12, 0.06. Mu.L of primer 13, 0.06. Mu.L of primer 14, 0.05. Mu.L of primer 15, and 0.05. Mu.L of primer 16.
Optionally, before the RHD gene primer is diluted to the working concentration, the method further comprises: carrying out high-speed centrifugation treatment on the RHD gene primer;
And diluting the treated RHD gene primer to the working concentration by adopting an EB buffer solution.
According to a third aspect of the present disclosure, there is provided a primer mix amplification method comprising:
sequentially adding reagents into the primer mixed system: sterilizing distilled water, deoxynucleotide, primer Mix, PCR enzyme, enzyme-free sterile water and genome DNA to obtain mixed solution;
amplifying the mixed solution to obtain an amplified product.
Alternatively to this, the method may comprise,
the amount of the sterilized distilled water is as follows: 12.5. Mu.L;
the amount of deoxynucleotides is: 5. Mu.L;
the amount of the primer Mix is as follows: 0.48 μl;
the amount of the PCR enzyme is as follows: 0.5. Mu.L;
the amount of the enzyme-free sterile water is as follows: 6.52-XμL;
the amount of genomic DNA is: x μl;
the total amount of the reagent was 25. Mu.L.
Optionally, amplifying the mixed solution to obtain amplified products, wherein the amplified working parameters are as follows: 94 ℃ for 2min;97-99 ℃,12s,67-69 ℃,12min,26 cycles, 11 th cycle starting, 30s increase per cycle; 67-69 ℃ for 10min.
Alternatively, the amount of genomic DNA is: in Xμl, X is 45-55ng.
According to a fourth aspect of the present disclosure, there is provided a method of quality testing an amplification product, comprising:
Detecting the amplified product by agarose gel electrophoresis to obtain a detection result of whether the target gene in the amplified product is amplified or not; wherein, detect voltage: 150V; detection time: 50min.
According to a fifth aspect of the present disclosure, there is provided a sequencing library construction method comprising:
the amplified product and the connector are subjected to connection reaction to construct a library;
purifying the library;
and (3) mixing the purified library to construct the RHD gene sequencing library.
Alternatively, the conditions of the ligation reaction are: 36-38deg.C for 40min;15-17 ℃ for 40min;64-66 deg.C for 10min.
According to a sixth aspect of the present disclosure, there is provided a sequencing method, which is to sequence the RHD gene sequencing library constructed by the construction method of the RHD gene sequencing library.
Alternatively, the RHD gene sequencing library was sequenced using HiFiads mode, wherein the loading was 150pM and the average fragment size was 5000bp.
The RHD gene primer, a primer mixed system amplification method, an amplification product quality detection method, a sequencing library construction method and a sequencing method, wherein the primer sequence comprises the following components: primer 1: RHD1-F, primer 2: RHD1-R, primer 3: RHD2-F, primer 4: RHD2-R, primer 5: RHD3-F, primer 6: RHD3-R, primer 7: RHD4-F, primer 8: RHD4-R, primer 9: RHD5-F, primer 10: RHD5-R, primer 11: RHD6-F, primer 12: RHD6-R, primer 13: RHD7-F, primer 14: RHD7-R, primer 15: RHD-BOX-F, primer 16: RHD-BOX-R; the primer mixture system is prepared based on the primer, the amplification product is obtained after the primer mixture system is amplified, a sequencing library is constructed based on the amplification product, and the HiFiready mode is used for sequencing the RHD gene sequencing library, so that the accuracy of a sequencing result can be effectively improved.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the disclosure and together with the description, serve to explain the principles of the disclosure. It will be apparent to those of ordinary skill in the art that the drawings in the following description are merely examples of the disclosure and that other drawings may be derived from them without undue effort.
Fig. 1 schematically illustrates components in an annealing process, components of an annealing system, and an annealing process schematic in an exemplary embodiment of the present disclosure.
Fig. 2 schematically illustrates the results of a two haplotype total loss gel in an exemplary embodiment of the present disclosure.
Figure 3 schematically shows a sequencing diagram of a full-length sequence of two haplotypes in an exemplary embodiment of the present disclosure.
Fig. 4 schematically shows a sequencing diagram of two complete haplotype sequences for one mutation site in an exemplary embodiment of the present disclosure.
Fig. 5 schematically shows a sequencing diagram of one rhesus box sequence and another complete haplotype sequence in an exemplary embodiment of the present disclosure.
Fig. 6 schematically shows a sequencing diagram of a 2 rhesus box sequence in an exemplary embodiment of the present disclosure.
FIG. 7 schematically shows a sequencing diagram of 1 haplotype complete and 1 haplotype recombination start and stop site sequences in an exemplary embodiment of the present disclosure.
FIG. 8 schematically shows a sequencing diagram of a 1 RheusBox sequence and 1 haplotype recombination start and stop site sequences in an exemplary embodiment of the present disclosure.
Fig. 9 schematically illustrates a graph of an analysis of the results of calculating average fragment sizes using a 5200Fragment AnalyzerSystem detection library in an exemplary embodiment of the present disclosure.
FIG. 10 shows amplification results of detection analysis of PCR reaction products by 1% agarose gel electrophoresis.
Detailed Description
Example embodiments will now be described more fully with reference to the accompanying drawings. However, the exemplary embodiments may be embodied in many forms and should not be construed as limited to the examples set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the example embodiments to those skilled in the art. The described features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
Interpretation of technical terms of the application:
RHD: rhD refers to the D antigen present on the surface of erythrocytes in the Rh blood group system, depending on whether the surface of erythrocytes contains D antigen or not.
gDNA: genomic DNA refers to the entire DNA of an organism in a haploid state.
And (3) PCR reaction: has the meaning commonly understood by those skilled in the art, which refers to a reaction (polymerase chain reaction) that uses a nucleic acid polymerase and primers to amplify a target nucleic acid.
EBbuffer: EB buffer (PH 7.2).
Annealing: in general, two nucleic acid sequences that are perfectly complementary or substantially complementary may hybridize or anneal. The complementarity required for hybridization or annealing of two nucleic acid sequences depends on the hybridization conditions, particularly the temperature, employed.
Exonuclease: exonuclease.
Primer: a primer; sequence: nucleotide sequence.
Conventional detection means in the art:
the current representative RHD gene detection typing techniques are mainly of the following types:
PCR sequence specific primer analysis (PCR-SSP):
the method has the most wide application in detecting RHD genotypes, the principle of the operation of the method is that according to the base specificity of the RHD alleles, sequence specific primers are designed, wherein the first base at the 3 'end of the primers is respectively matched with the specific bases of the alleles, and in the PCR reaction process, the complete replication of DNA fragments can be realized only when the first base at the 3' end of the primers is complementary with the base for determining the specific alleles, and the existence of PCR products is detected by agarose gel electrophoresis to carry out the typing of the alleles. The method has the characteristics of simple operation, low experimental cost and short time effect, has an initial interpretation on the result, can only analyze known specific alleles, but cannot detect new alleles, cannot be well distinguished from RHCE genes with higher homology, and can have more phenomena of false positive and false negative.
2. Nucleic acid Sequencing technology (PCR-Sequencing-BasedTYping; PCR-SBT):
i.e. the sanger generation sequencing technology, the method mainly comprises the steps of amplifying specific fragments of RHD genes by PCR, and carrying out DNA sequencing on PCR products to analyze the allelic types of the PCR products. In contrast to SSP technology, the first generation sequencing can directly obtain the gene sequence information of the relevant region by sequencing, so that the typing accuracy is improved, and even new alleles can be directly found. Because the sanger generation sequencing technology can only obtain 600-800bp of sequence information at a time, and the total length of RHD genes is about 58Kb, if all RHD gene information is required to be obtained, multiple positions are needed to be respectively designed and sequenced, so that the cost is too high and the operation is complex, therefore, multiple detection exon areas are taken as main streams in the market, the detection results of single RHD positive, RHD gene complete deletion negative and D variants caused by single SNP site mutation or negative and D variants caused by common mutation of a plurality of SNP sites on the same exon are more accurate, but due to the limitation of the method, the haplotype of a sample cannot be distinguished, the exchange among the SNP combined mutation of a plurality of exons cannot be well distinguished, so that the hybrid sample can generate a model-edge two-possible result, and the judgment cannot be accurate.
3. Next generation sequencing technology (NGS):
the present market mainly uses an NGS sequencing platform of Illumina company, the technology is that specific oligonucleotide sequences (i.e. linkers) are connected at two ends of RHD gene products, the products are loaded on a flowcell with complementary linker sequences on the surface, DNA replication is carried out by bridge polymerase chain reaction, signals are amplified, optical signals are converted into base sequences, and high-speed and large-flux sequencing is realized. The method has high flux, so that the cost of a single sample is relatively low, and new alleles can be found; however, the technology can only obtain the length of 150-250bp, so that the original sequence obtained by sequencing needs to be further assembled to obtain complete RHD gene information, the process needs to be calculated by a statistical method, haplotypes cannot be well distinguished, and therefore, the problems of combined mutation of exons, homologous gene exchange and the like cannot be completely solved, and therefore, partial ambiguous and erroneous results are also often obtained.
Due to the complexity of RHD genotyping and the limitations of the conventional detection technology, rapid and accurate genotyping cannot be realized, and inaccurate and ambiguous results often cause hemolytic transfusion and neonatal hemolytic disease, so that proper blood selection can avoid the generation of alloantibodies, and the prevention of delayed hemolytic transfusion reaction and neonatal hemolytic disease is particularly important. Therefore, the technical scheme of the application breaks the defects and bottlenecks of other methods by developing a new technology, so that RHD genotyping is more accurate and has higher sensitivity.
Based on the problems in the sequencing technology in the third step, the scheme designs a plurality of pairs of amplification primers according to the complex gene structure of the RHD, so that the RHCE genes with higher homology can be accurately distinguished. The 2-tube multiplex amplification is realized by researching and developing the most proper primer proportion, screening reagent raw materials and amplification reaction conditions; the PCR product adopts a self-developed library building system to obtain a third-generation sequencing library; finally, pacificBicosciences third-generation sequencing technology is adopted, and a self-developed analysis flow and software are matched, so that the RHD genotyping of the experiment is high in coverage, sensitivity and accuracy.
The current gene sequencing technology is robustly brought into the latest third generation sequencing technology (Third GenerationSequencing; TGS), the gene sequence reading length is more than 10,000bp, which is far higher than SBT and NGS, and the current market is mainly based on the sequencing platforms of PacificBisciences and Oxford NanoporeTechnology. PacificBisciences' single molecule sequencing in real time (SMRT) mainly uses several apertures in Zero-mode waveguide (ZMW) to detect fluorescence generated during DNA polymerization and repeat sequencing through circular DNA. The nanopore sequencing method (nanopore sequencing) of OxfordNanopore Technology mainly transmits potential difference across a membrane, and when different bases on single-stranded DNA pass through transmembrane proteins, currents with different magnitudes can be generated, so that the aim of sequencing in real time is fulfilled.
According to the application, a sequencing technology platform of PacificBicosciences is selected, the HiFi sequencing mode can reach the accuracy QV30 (99.9%) of the next machine data, and the single-piece length of the single-piece sequencing can reach 15-50kb. The method effectively solves the problems of short plates with low accuracy and short sequencing length in the traditional sequencing method, can obtain complete RHD gene sequences only by little assembly, and effectively improves resolution and accuracy. At present, no product of PacificBisciences technology is applied to RHD genotyping detection in the market, so that the technical scheme of the application develops a series of RHD genotyping flows through the technical advantages.
The technical scheme provided by the application comprises the following steps of: primer design, PCR amplification, product quality inspection, library construction, library purification, library mixing and on-machine sequencing.
First, the primers provided by the application are described in detail:
see table 1 for:
TABLE 1
In combination with the above table, the present disclosure provides an RHD gene primer, the primer sequence of which includes: primer 1: RHD1-F, nucleotide sequence:
CACAACCTGCGAAGCACGGGA；
primer 2: RHD1-R, nucleotide sequence:
CCTCGCCCACAGCCTCTACTCTACC；
primer 3: RHD2-F, nucleotide sequence:
GATGGTGTCAGGGGAGGGAGGTG；
primer 4: RHD2-R, nucleotide sequence:
AACAGAACCAAATCAGTCCCAAGTCAAGT；
Primer 5: RHD3-F, nucleotide sequence:
ATTCTGGCAACCTAATGAAGGAGTATGA；
primer 6: RHD3-R, nucleotide sequence:
GCTCCTTGACCGCTCGTCTCTTT；
primer 7: RHD4-F, nucleotide sequence:
AACTCACCAGAATACAGAACTCAGTCTCACA；
primer 8: RHD4-R, nucleotide sequence:
CTTCAGCCAAAGCAGAGGAGGTTAG；
primer 9: RHD5-F, nucleotide sequence:
TCAGTGTAAGAACCATAAAGGTGTATCTGTGTAGT；
primer 10: RHD5-R, nucleotide sequence:
TAGTTTTCTTATCAGTCGGTAGTTGTGTCA；
primer 11: RHD6-F, nucleotide sequence:
CAGGACTGTTGGGATGCTACTGGATTT；
primer 12: RHD6-R, nucleotide sequence:
CCAATAAAAGATGTTTGCTTGTAGAATAAAGGTGA；
primer 13: RHD7-F, nucleotide sequence:
GTTTTGATAACCTGAACTGTGTTTGGAGC；
primer 14: RHD7-R, nucleotide sequence:
GCAGTCTGTTGTTTACCAAGATGTTGTTATGT；
primer 15: RHD-BOX-F, nucleotide sequence is:
AAACAAATACCTGAAACACCTACACTACAAAAATG；
primer 16: RHD-BOX-R, nucleotide sequence is:
TGTAAGCCGAGCAGTAGAGACCTGAGAT。
in this example embodiment, the RHD gene primers were synthesized using primer 1-primer 16, all based on 5' phosphorylation. The primer sequence provided by the application is a specific amplification primer designed for RHD genes. Further, the present disclosure provides a primer mixture system, wherein the RHD gene primer is diluted to a working concentration and formulated according to a predetermined ratio, and the working concentration is: 100uM; the primer mixture system comprises:
0.03 to 0.05. Mu.L of primer 1, 0.03 to 0.05. Mu.L of primer 2, 0.05 to 0.07. Mu.L of primer 3, 0.05 to 0.07. Mu.L of primer 4, 0.04 to 0.06. Mu.L of primer 5, 0.04 to 0.06. Mu.L of primer 6, 0.02 to 0.04. Mu.L of primer 7, 0.02 to 0.04. Mu.L of primer 8, 0.04 to 0.06. Mu.L of primer 9, 0.04 to 0.06. Mu.L of primer 10, 0.04 to 0.06. Mu.L of primer 11, 0.04 to 0.06. Mu.L of primer 12, 0.05 to 0.07. Mu.L of primer 13, 0.05 to 0.07. Mu.L of primer 14, 0.04 to 0.06. Mu.L of primer 15, 0.04 to 0.06. Mu.L of primer 16.
As a preferred embodiment, the amount of each primer in the present application may be: 0.04. Mu.L of primer 1, 0.04. Mu.L of primer 2, 0.06. Mu.L of primer 3, 0.06. Mu.L of primer 4, 0.05. Mu.L of primer 5, 0.05. Mu.L of primer 6, 0.03. Mu.L of primer 7, 0.03. Mu.L of primer 8, 0.05. Mu.L of primer 9, 0.05. Mu.L of primer 10, 0.05. Mu.L of primer 11, 0.05. Mu.L of primer 12, 0.06. Mu.L of primer 13, 0.06. Mu.L of primer 14, 0.05. Mu.L of primer 15, and 0.05. Mu.L of primer 16. Wherein the mixed system is divided into two types, and the first multiplex amplification is as follows: primers 1, 2, 5, 6, 9, 10, 13, 14 were mixed and the second multiplex amplification was: primers 3, 4, 7, 8, 11, 12, 15, 16 were mixed. In this example embodiment, see table 2 for:
primer name Primer working concentration (uM) Primer mixture ratio (μL)
RHD1-F 100 0.04
RHD1-R 100 0.04
RHD2-F 100 0.06
RHD2-R 100 0.06
RHD3-F 100 0.05
RHD3-R 100 0.05
RHD4-F 100 0.03
RHD4-R 100 0.03
RHD5-F 100 0.05
RHD5-R 100 0.05
RHD6-F 100 0.05
RHD6-R 100 0.05
RHD7-F 100 0.06
RHD7-R 100 0.06
RHD-BOX-F 100 0.05
RHD-BOX-R 100 0.05
TABLE 2
Before the primer provided by the application is used, 5' phosphorylated synthetic primer dry powder is subjected to high-speed centrifugation, and the high-speed centrifugation can be realized by adopting high-speed centrifugation equipment; then, the mixture was diluted to the working solution concentration using EBbuffer, and the diluted primer was used to prepare primer MIX according to the system of Table 2.
Further, before the RHD gene primer is diluted to the working concentration, the method further comprises the steps of: carrying out high-speed centrifugation treatment on the RHD gene primer; and diluting the treated RHD gene primer to the working concentration by adopting an EB buffer solution.
As shown in Table 2, the working concentration was 100. Mu.M. In the present application, the mixture ratio of each primer in Table 2 may be prepared as a mixed primer system.
Further, the present disclosure provides a primer-mix amplification method comprising:
sequentially adding reagents into the primer mixed system: sterilizing distilled water, deoxynucleotide, primer Mix, PCR enzyme, enzyme-free sterile water and genome DNA to obtain mixed solution; amplifying the mixed solution to obtain an amplified product.
Specifically, after the primer MIX is prepared, the reagents are sequentially added into the reaction tube, and the tube cover is buckled, mixed and centrifuged.
In this example embodiment, see table 3 for:
TABLE 3 Table 3
In the table, PCRBuferforKOFDFXneo is sterilized distilled water, dNTPs are deoxynucleotides, KODnoFX is a high-efficiency and high-success rate PCR enzyme, DNase/RNase-Free DeionizedWater is enzyme-free sterile water, gDNA is genomic DNA, and Total is the Total amount of reagents.
It can be seen that table 3 shows the amounts of the respective reagents in this example, wherein the amounts of the sterilized distilled water are: 12.5. Mu.L; the amount of deoxynucleotides is: 5. Mu.L; the amount of the primer Mix is as follows: 0.48 μl; the amount of the PCR enzyme is as follows: 0.5. Mu.L; the amount of the enzyme-free sterile water is as follows: 6.52-XμL; the amount of genomic DNA is: x μl; the total amount of the reagent was 25. Mu.L.
In this example embodiment, the step of amplifying the mixed solution to obtain amplified products, where the amplified working parameters are: 94 ℃ for 2min;98 ℃,12s,68 ℃,12min,26 cycles, 11 th cycle starting, 30s increase per cycle; 68 ℃ for 10min.
Specifically, referring to table 4, table 4 is the parameters of the amplification reaction:
TABLE 4 Table 4
In this example embodiment, the amount of genomic DNA is: in Xμl, X is 50ng.
In this example embodiment, the PCR apparatus was programmed to amplify according to the parameters set forth in Table 4 above, with a thermal cover of 105℃and a temperature ramp rate of 6.0℃per second.
In the present example embodiment, the present experimental protocol designed and realized a set of 2-tube multiplex PCR-amplified RHD gene complete region technology. In the amplified part, the technology for realizing multiple amplification by the technical scheme of the application achieves the aim of amplifying the RHD complete gene with 58Kb by a 2-tube, so that the experimental process is simpler and more convenient. By primer design, the amplified region extends to the rhesus box region. Can effectively provide complete RHD gene information. Due to the technical characteristics, the technical scheme of the application obtains all RHD information (comprising an exon region and an intron region), most RHD genotyping only needs to use the exon region information at present, but the technology of the technical scheme of the application can provide more comprehensive intron information, and provides a complete solution for further researching the RHD gene function in the future, which is not provided by the traditional technology.
According to a fourth aspect of the present disclosure, there is provided a method of quality testing an amplification product, comprising:
detecting the amplified product by agarose gel electrophoresis to obtain a detection result of whether the target gene in the amplified product is amplified or not; wherein, detect voltage: 150V; detection time: 50min.
The amplified product is subjected to detection analysis by 1% agarose gel electrophoresis to determine whether the target gene is amplified. The voltage was 150V for 50min and FIG. 10 shows the results of the example amplification. (5. Mu.L amplification product+5. Mu.L 6 Xloadingbuffer) run gel
Referring to fig. 10, a in fig. 10: hole sites 1, 2 and 3 are the gel results of multiplex amplification 1, respectively, and the fragment sizes are mainly concentrated near 12Kb and 13.5 Kb; the positions 4, 5, 6 are the gel results of multiplex amplification 2, with fragment sizes centered around 10Kb and 12Kb, respectively. If two haplotypes are complete, 1 haplotype complete+1 haplotype deletion, 1 haplotype complete+1 haplotype recombination and 1 haplotype deletion+1 haplotype recombination appear in the sample, the two haplotypes are identical or similar to those shown in FIG. 1. M is Marker.
Fig. 10B: the positions 1 and 2 are the gel results of multiplex amplification 1 and multiplex amplification 2, respectively, with the fragment sizes mainly centered around 11 Kb. If both haplotypes were missing in the sample, they were identical to those shown in FIG. 2. M is Marker.
According to a fifth aspect of the present disclosure, there is provided a sequencing library construction method comprising:
and (3) connecting the amplified product with a connector to construct a library.
The library was purified.
And (3) mixing the purified library to construct the RHD gene sequencing library.
In this example embodiment, the Barcode joint is annealed for later use according to a conventional PacBio joint annealing procedure. PacBio joint annealing procedure referring to FIG. 1, FIG. 1 shows the composition of the annealing procedure, the composition of the annealing system, and the annealing procedure, in combination with FIG. 1:
the composition in the annealing procedure included: tris-HCl (Chinese name: tris (hydroxymethyl) aminomethane; tromethamine; ammonium bradycardia; tris, english name: tris (hydroxymethyl) aminomethane) pH 7.5) 100mM, naCl1M, NFW.
The annealing system comprises the following components: the amount of 10Xannealingbuffer (annealing buffer solution (10X)) is: 10. Mu.L; BGBarcode adapter (also called index), a very short strand of oligo-nucleic acid for labeling different samples when multiple samples are mixed sequenced), 20. Mu.L; NFW the system was supplemented with Upto 100. Mu.L.
The annealing procedure is as follows: 80 ℃ for 2min; decreasing at 80 ℃,0.1 ℃/s, and 2min; reducing at 75 ℃ and 0.1 ℃/s for 2min; reducing at 70 ℃ and 0.1 ℃/s for 2min; reducing the temperature at 65 ℃ and 0.1 ℃/s for 2 minutes; reducing at 60 ℃ and 0.1 ℃/s for 2min; decreasing at 55deg.C, 0.1deg.C/s for 2min; reducing the temperature at 50 ℃ and 0.1 ℃/s for 2 minutes; 45 ℃, reducing the temperature by 0.1 ℃/s for 2 minutes; reducing the temperature at 40 ℃ and 0.1 ℃/s for 2 minutes; reducing the temperature at 35 ℃ and 0.1 ℃/s for 2 minutes; reducing the temperature at 30 ℃ and 0.1 ℃/s for 2 minutes; reducing the temperature at 25 ℃ and 0.1 ℃/s for 2 minutes; 4 ℃, and ending. The annealing procedure was about 45min and the annealed junctions were stored at-20 ℃.
In the present exemplary embodiment, the ligase Mix was prepared and arranged in advance in the adaptor ligation reaction according to table 5 below.
Reactive reagent Single sample
10×T4DNALigaseBuffer 1.5
dNTPMix(10mMeach) 0.1
dATP(100mM) 0.1
T4DNAPolymerase 0.3
T4PolynucleotideKinase 0.3
T4DNALigase(Rapid) 1.25
Total 3.55
TABLE 5
The following table 6 shows the connection system: adding the materials in sequence, covering a pipe cover, mixing and centrifuging.
Amplification product 8.95μL(200～300ng)
Barcode joint 2.5μL
Ligase Mix 3.55μL
Total 15μL
TABLE 6
Table 7 below shows the procedure of the ligation reaction, after the parameters were set in advance, the ligation reaction was performed with a hot cap at 75deg.C and a heat rate of 6deg.C/S.
Temperature (temperature) Time Cycle number
37℃ 40min 1×
16℃ 40min 1×
65℃ 10min 1×
4℃ ∞
TABLE 7
And (3) enzyme digestion reaction: 1. and (3) a circumscribed system: incorrect ligation may occur during ligation, and thus incorrect ligation products are digested by exonuclease. The exonuclease MIX was prepared on ice according to table 8 below, and when the ligation reaction was completed, 2 μl of exonuclease MIX was added to each tube, covered with a tube cap, and centrifuged.
Reagent name Single sample addition volume (μL)
ExonucleaseⅠ 1.5μL
ExonucleaseⅢ 0.5μL
Total 2μL
TABLE 8
2. And (3) performing an circumscribed program: table 9 below shows the parameters of the digestion reaction, which were set in advance, and the digestion reaction was performed with a heat cover at 45℃and a temperature rise/fall rate at 6℃per second.
Temperature (temperature) Time Cycle number
37℃ 60min 1×
4℃ ∞
TABLE 9
After the digestion reaction is completed, the magnetic beads must be purified as soon as possible.
In this example embodiment, the step of library purification comprises:
1. taking out VAHTSTMDNACleinBeads (hereinafter referred to as magnetic beads) from the refrigerator half an hour in advance, and slowly mixing at room temperature on a vertical mixer for 30min;
2. adding DNase/RNase-FreeDeionized Water with volume 1 times to the digested product, adding 15.3 μl magnetic beads (0.45 x), mixing with flick or shaking at low speed, standing at room temperature for 10min, and mixing with flick for 2-3 times;
3. the PCR tube is instantaneously separated and then placed on a magnetic rack to adsorb magnetic beads for 10min, 70% alcohol is prepared during the process, and the alcohol needs to be prepared on site;
4. discarding the supernatant, adding 200 mu L of 70% alcohol along the opposite side tube wall of the magnetic bead, and eliminating the magnetic bead;
5. repeating the previous step of operation, and performing the second alcohol cleaning;
6. discarding alcohol, centrifuging the PCR tube, then putting back to the magnetic rack, discarding residual liquid;
opening the cover of the PCR tube, drying for not more than 30s, and then adding 12 mu LEBBuffer;
8. Lightly flicking and uniformly mixing the magnetic beads, keeping the magnetic beads of the system in a uniformly mixed state, and standing for 10min at room temperature for 2-3 times;
9. the PCR tube is instantaneously separated and then placed on a magnetic frame to adsorb magnetic beads for 10min;
10. transferring 10 mu L of the supernatant into a new PCR tube;
11. single sample libraries were quantitated using Qubit, and each single sample library was taken as a mixed library with the same mass of 6 ngpooling.
In this exemplary embodiment, the step of mixing the library includes:
1. the exact volume x μl (> 400 ng) was measured with a gun for the mixed library, to which x 0.6 μl magnetic beads (0.6 x) were added,
the purification process is the same as the library purification process, and finally, 50 mu LEB is adopted to elute the library;
2. the library obtained in the previous procedure was taken in x μl (> 200 ng), to which x 0.45 μl magnetic beads (0.45 x) were added,
the purification process is the same as the library purification process, and finally, 20 mu LEB is adopted to elute the library;
3. the final library was quantified using Qubit, requiring triplicate, taking the mean as the final library concentration, requiring final library concentrations above 3 ng/. Mu.L.
4. The library was tested using a 5200fragmentAnalyzer System and the average fragment size was calculated, FIG. 9 below is the analysis result.
According to a sixth aspect of the present disclosure, there is provided a sequencing method, which is to sequence the RHD gene sequencing library constructed by the construction method of the RHD gene sequencing library.
Further, pacificBisciences sequencing platform uses HiFiready mode to sequence the RHD gene sequencing library, wherein the loading amount is 150pM, and the average fragment size is 5000bp.
In this example embodiment, due to the technical characteristics of the PacificBiiosciences sequencing platform, the obtained original data only needs to be assembled with a very small number of sequences to obtain complete information, so that the problem that the first and second generation sequencing technologies cannot distinguish sister chromosome information (phasing) and error information possibly caused by assembly can be avoided. The whole RHD typing technology is improved to a new level.
In this example embodiment, the amount of the upper machine of upper machine sequencing was 150pM, and the average fragment size was 5000bp using HiFiready mode.
In the present exemplary embodiment, the present technical solution is directed to a positive sample, and two full-length sequences with complete haplotypes can be obtained at one time (e.g., sample 1 and fig. 3); for a more exon combined mutation sample on 2 haplotypes, two complete haplotype sequences of the mutation site can be obtained at one time (e.g. sample 2 and FIG. 4); for 1 haplotype deletion and 1 haplotype complete sample, 1 Rheusbox sequence and another complete haplotype sequence can be obtained at one time (e.g., sample 3 and FIG. 5); for 2 haplotype deleted samples, 2 Rheusbox sequences were obtained at a time (e.g., sample 4 and FIG. 6); for 1 haplotype complete (or partial RHCE recombination) and another haplotype recombination sample, 1 haplotype complete (or partial RHCE recombination) and 1 haplotype recombination initiation and termination site sequences (e.g., sample 5 and FIG. 7) can be obtained; for 1 haplotype deletion and 1 haplotype recombination sample, 1 RheusBox sequence and 1 haplotype recombination start and stop site sequence can be obtained at a time (e.g., sample 6 and FIG. 8).
The technical scheme utilizes the advantages of long length and high accuracy of PacificBicosciences third-generation sequencing, can completely detect new alleles, assembles haplotypes and accurately parting, and ensures that various types are not shaped; meanwhile, the scheme has large detection flux, a plurality of samples are detected simultaneously, the experimental process can realize automation, and manpower, material resources and financial resources are greatly reduced.
In this example embodiment, the operational flow and sequencing results are illustrated:
the amplified product is subjected to library construction process to obtain a library, data obtained after the library is subjected to machine sequencing are converted into HiFi data with high accuracy, mutation site information and structural variation information are obtained through data analysis, and finally the mutation site information and the structural variation information are compared with a standard database to obtain corresponding typing. The types of samples sequenced are listed in the following table, which illustrates only representative haplotype types, and the present patent can detect various types such as the new types listed in ISBT and not shown in ISBT:
the following further describes the technical scheme of the application with reference to the detection sequence:
type 1: haplotype is complete (61,700 base pairs, 10 exons are all complete)
CACGCCAAGCCGGGAAGTCCCCGCCTCCTGGAGCTGAACCCGCCCCTCTCCCAGAGGTGGAG
CTGCGGGGGGCGGGAACAGGCACGGAGAAAATAAACAAGACTAAAAAGTCCTGAGTAGCGC
TGTGTGGCCGCAAACCTGAACCCACCTTTTGCACCACGCGGGACCCGGCACGCTTCCTGCCA
CCCACCCCTGAGAGGGCTGCGCGGCCGACCCCAGTACTAGAAAACACTCGTCACCTCAATCA
AGACGGGTACGAAGGCCAACGGACGCCTTCCTTTAGAACGCTCAGCACACAGAGCAACTTCT
CACGCCTACTCTCAAATGGCGTACTCCAAACTAGCACTCCCGACGTCCAGCTGTGAACCCAGA
GCGGCGGAAAGCCCCTGAACCCAGCGCCCGGGCATGCGCAGACGCGTTGTTGTGGTGGGCGT
GGCTCCCTCCGGACCCGGCGCCCCGCCCTCCGCCCCGTGTCCGCATGCGCGACTGAGCCGCG
GGGGTGGTACTGCTGCATCCGGGTGTCTGAAGATCCGATGAAATAACATATGCAAAATGATTG
GGTCCGTGATTGGCATTCCAGAAATGGTAGCTGTTATTCAGCCAACAAATATTTATTGAGCACC
TACTATGGACTTCCCTGGTGCTGAGGATACAACAGCAACCACAGCAGTCAAAAGTCCCTGTCT
TCATGTTGCTCAGATTCTCATAGGGGAAAGCAAATAATGAACAAATACACGGCCGGGCGCAGT
GGCTCACGCCTGTAATCCCAGTACTTTGCGAGGCCAAGGTGGGCAAGTCACCTGAGGTCAGG
AGTTCGAGACCAGACTAGCCAACGTGGTGAAACCCTGTCACTACTAAAAATACAAAAATTAG
CGCGGTGTGGTGGCTCATGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGAAGGAGAATCGC
TTGAACCTAAAAGGCAGAAGTTGCAATGAGCCAAGATCGTGCCACTGCATTCCAGCCTGGGT
GACAGAGTACTCCGTCTAAAAAAAAAACCTAAATACACAAGTAAAAATATAGACCTCGTCAGA
TGCTAGTAAGTGCTGTGAAGGAAACTAAAAGGGGAACACAAGGAACCCTTGTCAAGGGGAG
AAGAAAGGGGAGTTGATGCTGTCCTTTTAAATAGGGCAGTCAGAGGCCGGGCACAGTGGTTC
ACACCTATAATCCCAGCACTTTGGGAGGTTGAGGTGGGTGGATCACTTGAGGTCAGGAGTTCA
AGACCAGCCTGGCCAACCTGGTGAAATCCTGTCTCTACTAAAAAAACAAAAACTAGCCGGGT
GTGGTATCACGCGCCTATAATCCCAGCTACTCGGGAGGCTGAGGCGGGAGAATCACTTGAACC
TGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGTGCAATTGCAGTCCAGCCTAGGCAACAAGA
GCAAAACTTCATCTCAAAAAAAAAAAAAAAAATAGGGCAGTCAGGGAAAACTTTCCTGAGA
AGGGGATGGTGGAGGATCCAGGGAGGTGAGGTGGGGAGCAAGCCAGTACAGTTGTTCCTTG
ACTTTCGATGGGGTTATGTCCTGATAAAGCCATGGTAAGTAGGAAATATTGTAAGTCAAAAATG
CATTTAATACATCTAACCTACGGAACATCATAGCTTAGTCTCACCTACCTTAAACATGCTTAGAA
CACTTACATTAGCCTACAGTTGGGCAAAATCCTCTAACACAAAGCCTATTTTATGATAAAGTATT
GAATATCTCATGTAATGTACTGAGTACTGTACGGAAAGTGAAAGACGGAGTGGTGGGATGGGA
ACTCTAAGCGCGGCTTCCACTGCATGTGTGTTGCTTTCGCGCCATCATAAAGTTGAAAAGCGTT
AAGTCAAACCATCGTACGTCGGAGGCCATCTGTATCTGGTAGGAGGAGTGTTTCAGACAGAG
AGAACAGCAGGTGCACAGAGTGCTTTTTTCCCAGCATTTTATTATGAAAAATTTCAAACATCTA
CCAAAAAAAGTTGAAAGACTTGTACAGTGAAAAGCCATACATCTCACAGCTAGAATCAACAA
TTAACATTTTACTGTATTTGGTTTTTGACTTATCTATCCTAGATCCCTTGTGCTTTCTGTAGCAGG
TGACCTGCCTTGAAGATTTAAAGACAGAATATCAGGAAATGTAGTCAGAAAATGGGGCCTTTT
ATAAGAGTCAGAGGGGAAGAGCAAAACCTCTGCTTTTGACAAATCTGTTGGGAGAGGCCAAC
TGCAGGGATACCTCCCTTTTTTAATGAAAGCATTTCTGTTCTGCGAGGAGCGGGATCCTCTTGT
CAAGCAGTCAGTCCCTGCTGCTTCCTTACTGGGGCAGGATCAGGACGCACAGGGATTTGGAG
TGCCTTGGAACCAACCACCACCCACGCCGTTTGCCAGCTGGTAAACATGCCCATCAGGTCCGG
GGGTTGGCATTGCCTGGACATCTTTAGTGTTCATCTTGCTGACATCTGGTGCCCTCGGGCAGGT
AGGTGCAGTTGGCTGCCTGGTTTACAGAGCTTGTACTGGGCCCAGGTTAGCAGAGGTCACATC
CATTTATCCCACTGCGCAGAGGAGTTCCTTCTCAGGAAACCCAGTTTATAAGAAGTACTGACT
GCCAGAAATAGAGCAGAAATGAGAACCAGGAGGCAATTGTGAGAGGAATGGAGACTTCTGA
CCTCTGGGGATTGGGGTACCCTCCCCCTTAATTGCTGTTGGGGTAGCAGAGGGCTTAGAAGCC
CATGTTCCTAGACTTTTAGAATTGGAAGAAGACTTAGAAGTAATCTAGGCTGGGGGTCCCCAA
CCCCCAGGCTGTGGCCCGTTAGGAACCTGACCGCACAGCATGAGGGATAGGCCAGCGAGCAC
TACCGCCTGAGCTCCGCCTCCTGTCAGATCAGCAGCGGCATTAGATTCTCATAGGGGCACAAA
CCCTATTGGGAACCGCGCATGAGAGGGATCTAGGTTGCGTGCTCCTTAGGAGAATCTAACTAA
TGCCTGATGATCTGAGGTGGAACAGTTTCATCCCCAAACCATCCCTCCAACCTCACCCCGGTC
CATGGAAAAATTGTCTTTTACAAAACCCGTCCCTGGTGCCAAAAAGCTTGGGGACCCCTGATC
TAGGCTACAGTTAAGTGGTCAAACACCCAGGTCCTGAAGTTAGGCTGCCTGGGTTTAAATCCC
AGCTCTACTGCTTACTAGCCCTGTGACCTTGAGCAAGTCACTTAGTTTTTCTGTGCCTCAGTTC
ACTCATTTGTAATAAATCCTAATAGTACCCATCCCAGTGTCATGAACTAAGTTCATATATGTAAA
GTGCTTAGAATGGTGCCTAGCAAGTACTTAATAACAGTTAGCTCTGAAAATGTATAAAGCAAAA
TTAACCAATGTTTTAGTGGTTTGCAGCCAACTTTTTTCTATGCGTGTGCTAACATATTATTTTATA
AGAGTGGGAATATATTGTACATGCTGTTATATAACTTGCTTTTTCACTAAACAGTCTATCCTCTG
TGTCAGTTTTGATAAAAGCGTTTTCCTCTTGCTTTTCCTGCATATGTTCAGAACCATCATATTGG
TAGCAAGTTTCATGTCCTGTAGTTTTCTTAACCAACCCCCTGCTAGTGGACATTTAGGTTAGTC
TCAGTTTTTTCCTTCTGTAAATAAAGCTGCACTGAGCAAGAAGTGACTGATGCCAAGTGACTA
GATGACCTTAGGTATGACCTCTCTGGGTCTTGGTTTCTTGGTCTAAAAACAAAATGACAGGATT
CGACTGGGTGATTAAAATCTCCTCTGATCTACATAGGAATTGTTTTCAAGACATTTCTGCATTCC
TCTAGTGACAGGGTGCTCACTACCTCATGAGTATTTCAGTGGACAACTGTAATGGTCAATAAA
GTATCCACTTTCCACCTCCCTGCAGCTCCTGGCCCTGGCTTTATTCTCTGGGGCTCCACACATT
CAGTTTACACTCAGTGGCCAGTGGCTGGGACCATTGTAGAAAATAAGGAAACTCCAATTCCTT
CCTTCTTTTCTTCCTCTTTCATCTCTTCCTCCCTCTCTACATCCCTCTCTCTCTTCCTTCCTTCCT
CGACACTTACCATGTACCAGACCTTCTGCCAGGCACATGGATGGGAGCACAGGGGAAGTTGG
CTGCAGGGTTAGAACTAAGTCCCAAGCCCCCTAAAGCTCATGCCAGGGGACTGGACTGTCCA
GTACTGAGGGATGGGGATGCTGAGGCTGGTGGCCTTCCTCAAATGCACTGTAGTGCCCCAGGC
AGAGTCCTGGGCTGCCCTGTGAGGAGGTGACCAGAGGTAGAGCAACTTCACCCTAAGGCTGG
ATCAGGATCCCCTCCAGGTTTTTACTAGAGCCAAACCCACATCTCCTTTCTCTTCTGCCACCCC
CCCTTAAAATGCTTAGAAACACATAGATTTAAATACAAATTCAAATGTAAGTAATTTCAACTGT
GTAACTATGAGGAGTCAGTTCTACGTGGGTCCTATCTGTATCCTCCCCAGGGCTCAGCTCCATT
CTTTGCTTTCATTCATTCTCATTCAATACATTGTTGTTAAGAGCTCACTGGGTGCCCTCTCTGTC
ATGTAGTAAGGTTTTAAAAAGAAAGCCTCTTCTGAGCTTCAGTTTCCTTATTCATAAAATAGGA
GTATTGATCCGTTCCTTGCTTTTCTTACAAGGATATGCTGAAGATGACTGAAGTACAGAGTAAA
GAAGGATTATGTTTGGGTGTCAAAGGAATAGAATGCCCTCTTTCAAACTGAGCACAGCAGGA
ACCTGTAACAGGAACACAGCAACTTGTTGAATGAATGACAATATTGGAAAACATACATTTCCT
CCCCTCCCCATCATAGTCCCTCTGCTTCCGTGTTAACTCCATAGAGAGGCCAGCACAACCAGC
CTTGCAGCCTGAGATAAGGCCTTTGGCGGGTGTCTCCCCTATCGCTCCCTCAAGCCCTCAAGT
AGGTGTTGGAGAGAGGGGTGATGC
The "CTGGTGCTGGTGGAACCCCTGCACAGAGACGGACACAGGATGAGCTCTAAGTACCCGCGG TCTGTCCGGCGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTCTCATTCTCCTCTTCT ATTTTTTTACCCACTATGACGCTTCCTTAGAGGATCAAAAGGGGCTCGTGGCATCCTATCAAG" sequence segment is RHDEXon1"
GTGAGAGTTCATTGGAAAAGTGGTCACAGGAGCAAATAGCAGGGGCAGGGGCGGGGGAGGC
CTGTGGTTCTCCAGGGGCACAGATGTTCCTTTCTACAAAATCCCAAGGAAAAAGATTCCCCCA
TCTTCTTCCGTAGATTGCACCGAAATTCAGCCAACAATGTAAGCTTTCCTTTAGAAGCAGCCTG
GGCATGCCCTCTTCTGTGAAGCCTGCCTTGATTTTTCAGCACAGTGAGAGGCATCCTCTTTGGT
GTTCCTCAAATTCCCTCTACCAAATGGTCTTCATAATTCTCTGCTTCTCTGCTTCCCCTTCTCTC
TCCTCAGTGGCAAGGAATTTTTTTATTTTTATAGATTTAGGGGATACAAGTGCAGCTATCTTATG
CAAGCAATTTCATGTTGTTGGGTTTTTGGTTTTTGTTTCCTTTTTGTGGCCTCTCGCTCATTTCT
TATTTCTTTTTGAGGCAGGGTCTCACTCTGTTGCCCAGGCTGAAGTGCAGTGGCATGATCATGG
TTCACTGCAGCCTTGACCTCCTAGTCTCAAGCAATCTTCCCACCTCAGCCTCCCAAGAAGCTG
GGACCACAGGAGGGCACCACCATGCCTGGCTAATTTTTTTTTTTTTTTTTTTTGGTAGAGATGT
GGGTCTCCCTGTGTTTCCCAGACTGGTCTCAAACTCCTGGACACAAGCGATCCTCCAGCCTCA
GTCTCCCAAAGTGCTGGAATTACAGGCGTGAAGCACTGTGCCCAGCTCTCTTGCTCATATCTAT
ACTAGTTTTCTTTTGGAAGCTTCAGCCTGTTGCTACCCCCCACCCCCACCCCCACCGACCCCA
GCTTTCTTCTCACTTAGGGGCTGGGAAGTCTGCATGCTGTCTATAAATCCAGAACCAGAAGGT
ATGGCTGAAGGGGAGGGTAGGATGATGGTTATTTTATATTCAGCTAAAAATATTCCCAGACTGT
GATGAGACAACTGTAAATAAGACAGATGTCCACAATGGTGTGACTTTGCTTTTTTAAAAATATT
GAAATGAGTTTCAGGCATCTCAGTGGGCTGATAGGTTGTTGATAATAGACAGGGCCTCCTTGA
AGAATGTCCCTGAGACAAAGTTGAAGCTTGAGCCTGGTTGAGTCCTTGCTTGTTCCTAGGTTG
ATATGAACGGCTAGTTAACTGGAAGCAAAGAGAAGTCATCCTGGGGGCCATGGCAGTGACAA
GTAGGACTTAGGGAGGGAAGCCCTTATACCATTTAAGGTGCTGGCCCAGAGAGGAGCCTTCA
GTGACAGACAAACAAGAGCTGGCACAATTTTAATTCACTTCAATTTACTCTAATTCATTTCAAT
CCAATACAATTCAATGCATTCCATTCATTCAACCATGTATGACATCCAATGTGGGATCCAGACTC
ATGATGATTAGAGCTGATATTTATGAGCACTTACTATGTACCAGGCACTATTCTACATGCTTTACA
TTGAACCCTCACAATAACCCAATGAGGTGGGTACTATTATGATCTTCGTTTTTCATATGAGGAA
ACTAGGCATATGGATGTTGAGTAATTTGCCCACGGTCGCTCAGCTAGCAATAGCACAGCGTATT
TAAATTTAGCCACCCTGGATTTAGTTTCCTTACACTTAACCATTATGCATCATGGCCCCATTTTA
CAGTGGGCTTGAGTCTTTGTCATATAACCCAGTAGGTTAGCAGCCACTATTCCAACCCTGTAGA
TTGACTCTAGGGTCCATGTTCTTTACCCCTGCACCGTGCTACTAACGTAGGTACAAAATGTCCT
CAGAAACTCACTTTATACGGAAGCTCAGAGGAGGGTCCACAACCCAGGCAGGGGAGACGAT
GGTGTCAGGGGAGGGAGGTGACTGCCCAGCCAGGTCTTGAAGGCTCAGTAGGAATTACCTGT
GGGACAAAGGAGGGTCATCCAAGTGAGGGCACAGTGGGTGCCATGGCGTGCACACACAATA
GAGCAGACTGAGCCTGGGCTTAACATTGCATTGCCCTGGAGCCTAAAAGGGGAAACAAAGGG
CCGGGCGACGTGGCTCACGCCTGTAATCCCGGCACATTGGGAGGCCAAGGCTGGAGAATCAC
CTGAGGTTAGGAGTTCGAGACCAGCCTGGCCAACATGGCAAAACCGCATCTCTACTAAAATTA
TAAAAACTGGCTGGGTGTGGTGGCACACGTCTATAATCCGAGCTACTTGGGAGGCCATTACAC
TCCAGCCTGGGCGCCAGAGTGAGACTTCATCTCAAAAAACCAAACAACAAAAACAACAACA
AGAACAACAAAAAAACAAAGAGGAGAGCAGGGACTGGGTGTGGTGACTCATGCCTGTAATC
CCAAACACTTTGGGAGACCAAGGCAGGCAGATCACCTGAGGTCAGGAGTTCGAGACCAGCC
TGGCCAACATGGTAAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGATGTGGTGGCAC
GTGCCTGTAGTCCCAGCTGCTTGGGAAGCTGAGGGAGGAGAATTGCTTGAACCCAGGAGGCA
GAGGTTGCTGAGCTGAGAACATGCCACTGCACTCCACCCTGGGTGACAGAGTGGGACTCTGT
CTGAAAAAAATAATAGTAATAAATAAAAATAAAGAGGGAAGCAGCGGGTGGCAGACTCACTG
GGCTGCATACGAAGTTTGGCTTCAGTCTGAGGTCCGAATAGTAAACAGCAGCGAGACAAGTT
TGGGTTTGGGTCATGGAGGAAGCCATGCCAGGGCTGGTGTTGGGCACAGGGAAAGGGGCATG
GCTTGAGACACCAGACCAGCGTGGAGGCTGTAGTGTAGTATTGACCTGAGGACTTCAACATTC
TGATGGTGTACACACGATTTTTTGAGCATGTACCATGGTTATATATTACACTTTAAGTATTACTTT
AAGTATTACTACATTAATATATTTTGTATGTTACAATAAATACATACAAATTAGGAAAATTGAAAG
AGATCAAAATGAAATATATAATATTTTCAAATTACTAATCATAATGGTGTCAATCTCCAGGCAGG
GTCCATTGCTACAGTTGACGATAGTGGATGAAAATTCACTCCTCAGAGTCTTCTTGATAATTTG
AAATTGTCTTGATTGACTTGTCAGATCTGATTAGATCAACATGTTTTAAATCTCGAATGTGACTG
ACAGCTTGTACGAGGAGAAGTTTCACTCTGCCTTTTCCCTTTTGTTCACTTGACTGCCATTATT
TCTATGCTTCCAATCTGTGTTTTTCTGCACGAGTTGGTTAAGCCATTACTTCATTTTGTGAAAGT
TTGTTGAGTTAAACTTAGGTAACTTAATCTGTCAATCCACTTAATTGAATTCAGTCCTGGTAAA
CTATAATAGATTATTCAAACCTGCCAATTCTAAAAAGACATTTTGAGACAATCAGGAAATCTGA
ATATAGCATGAATATCTTACGATATACAAGGATTATTGTTAATTTTGTTAGGTATGATAAAAGCAT
GGTGGGTTGTTTTTGTTTTTGTTTTTTAAGTCTCCATCTGTTAGAGAGGCACATTGAAATGGCA
TGATATCTGGGGTTTGCTTTTATGCCAGAAAAAAGAAAAAGTACAGAAGGATTATAGAAACAA
GATTGGTCTCATGTGACAATCATCAGAGTTTGGAGATGGGCACGTAGGGTCATCGTGCTGTTCT
CTCTGTTTTCGTATATGCTTTAAAAGTTCTGTAATAGTTAATTAAAAAAAAAAAAAAACACCCT
GGCTGAGCATTTAGGGAGGCCAAGTGGGGAGGATCGCTTAAACCAAGGAGTTCAAGACGAG
CCTAGGAAACATAGGGAGACCCCCCCCATCTCTAAAAAAAAAAAAAAAAAAAAAAACTTTAA
AATTTAACCCAGTGTGGTGGCACATGCCTATAGTCCCAGCTACTCAGTAGGCTGAGGTGAGAG
GCTTGCTTGAGCCTGGGAGCTTGAGGCTGCAGTGGGACGGGATTGTACCACTTCACTCCAGCA
TGGGCGACAGAGCAAGACCCTGTCTCAAAAAAAATAAAAATATTTGAGGTGAAGCGAGGCTG
TAATAACAAATTTAAAAATATAAATAAAACATAAAGGCTGGGTGTAGTGGCTCACGCCTGTAAT
CCCAGCACTTTGGGAGGCCAAAGCAGGCAGATCACGAGGTCTGGAGATGGAGACCATCCTGG
CTAACACGATGAAACCCCATCTCTACCAAAAATACAAAAAAATTAGCCGGGTGTGGTGGCGG
GTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCG
GAGCTTTCAGTGAGCTGAGATTACGCCACTGCACTCCAGCCTGGGCAACAGAGCGAGAATCC
GTCTAAAAAAAAATGAAAATAAAAATAAATGAAACATAAAACCCTGCCATTAGTTGCAATATG
AAGAATATAGAGAAATGCATATCAAATCCTTCTCATTGGACCAATATTCCCTTAGGGCACCTTC
CAAAGCTAGGAGACTCAAGGCTGTATGACATCCTGAGCAAGTGAGGGGTGGCTTCTGGGTGA
ATCTGAATATTAAATATTTGCAGAATTGAAAACTTCACAAAGTACCTTTAGAGATAGAATAGCC
TAGATCCATGTTTCTCAAAGTGTGGTCCCCAGACCTGCTGCCTCAGCATCTCCTGGAAATTTAG
TAGAAATGCAGATTCTCAGGCCCTAGGCCAGACCTACTGATCAGAAGCTCTGGGCCTGGGGCC
CAGCAGTCTGTGTTTTCACAAGCCCTCTTGGTGATTCTTCTGTGCATGAAAGTTCGAGAATTCC
TGGAGCTAGACTGATTCAAATCTTGCCTCTGTATCTTAGAGACCTTGGGCAGATTAGTCAACCT
CTTTCTGCCTCTGTTTCTACTTCTGTCAGAGGATGATAGTACTTGTTTCATTAAGTTGTTGAAAG
GATAAATGAATTGACACACATAAAGAGTATTAGCTTTTATTATCAAAAGCTTTTTTTTTGAGACA
GAGTTTTGCTCTTATTGCCCAGGGGAGTGCAGTGGTGCGATCTTGGCTCACCGCAACCTCCGC
CTCCCAGGTTCAAGTAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGC
CACCACGCCCGGCTAATTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTGGTGAGGCTGG
TCTCGAACTCCCAACCTCAGGTGATGCACCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGG
CGTGAGCCACCGCGCCTGGCCCAAAAGCTTTAATTTCTTAATTTTTTAAATAAAATAAATAAAA
CTAGAATTGCTTGTTTTCTTCCAGCTACCCTGGTGATTGTATTGAGCATTTTCTGGGGTGTGTGT
TCTTTGCTGTAATGACTACTGGTCTGGATGACCTGTGATGAGACCAGATGGGCAGGGGCAGTG
GAGGAGATTCTAGAGATATTTAGGAGATAAGTCAGCTGTACTTGATGAAAAGAGTGGGGAGTT
AAGGCTGGCTGCAGATGTATGATTTGGCATAGAGAGGTGCCAGTTCCTGAGATGAGAGACAG
AAGGGGAGGGACAGGTTGTGAGGATGAATGAACAATGATATGTTCATTCTGGGCTTGGAGTTA
AGGGGCCTATGATATGCTTAGGGGAAGCAGAGAGTATCAATTACCTATTGCTGCATAACAGCCA
CCCCAAACTTAGTGGCTTAAAATAGTAACCTTTTAATTTACTCATGATCATGATTCTGTGGTGCA
ACAACTGGGCTGGGTTCAGCTGGGCAGTTCTTCTGTTAGTTTCACCCAGGGTCATTCATGCAT
CTGCAGTTTGGGGTGGGATGGCCTCAGATGACCTCATTCACGTGTTTGGCAGTTGGTGATTCA
CTGGGGGCCATTACTGTAACAATCGCCTACCAGGCAGAGCTTCCCTAAGGCTTCCAAACTAGG
AGACTATCCTGGGTCCTGTGCTGTGGATACCACTCAGTCCCCCATCCCCACCCCATATTCCTCA
AAGGCAGAGAGAGGGGCTACTAGAAGACAGAGGAGTTTTCCCAGTGACATGTAAACACTCCA
AACCCTGGCACCTTCCACACTGCAGCTTTGGTCTGCCCCTTTGGGAAATCTCTGTTTTTCTTCC
CAGGCTGCTGGAGGGGTGAGAGTCGCCGGTAGAGTAGAGGCTGTGGGCGAGGAGGTGGCGG
CCTCCTGAGGCTGCAGTGGTCTTTCCAGGCAGCAGTGGGAGCACAGGGTGGAGGTCAACCCT
AGAGCCTGGGAGAGTGAAGCTGGGTGTGACTTCAGAGCTGTTGGTGCTGAAGTTTCTGCAGG
CCAGAAGGAGGGGCAAGAGTGGGAGGGGGCGCAGATCCAGAATCACGGAGGCAGCTGACC
GGAGGAGGCAGCTGCCCAAGGGGATGGACTCAGAAGGCCAAAGTGCTGTTATCCAAACGAA
CTCTTTGCAAGTGGTCTCTTTGCAACAGGCCTGGGGGAGAGCAGTCTTGCCTAAAGTCACAC
CGCTAATCAGCGGCCGGCACGGGGTAACAGTTACTAACACTCACTACGTACCCAATGCTGGGC
GAAGTGACTTGCATGAGCCAGCGAGCTCAATGCTCATGGCAATCCTCTGAGCAGCTGGCATTG
TTTCATCTCAATTTTACAGCTCAGGAAGCTGGGACACAGAGGAAGAGCCAGGCTCTGAACAC
TGACAACCTGATTGAGAGACCCACACTGTTCATCACCGTTACGCTATATATGCTGTATAGAAAG
GCAGGATGGCATAATGGTTAAACCTAGGTAGGTAGGGTTTGAATCCTCCTGCTACCATTTACTA
GCTCTGTGACTTGGACTAGTTATAGCACCTCTCTGTGCCTCCCTTTCCCCATCTCTAAAATGGG
GATAATAAATCGTACCTCCTACCTGAGGCTGTTGTGGGCTAAGTCTGTAAGGCACGTAGAACA
GTGCCTGGAACGTGGGGTACTGTCTATCTGTGTGCCTGCTGTTACAACAATGGTGAGTATTGCC
TTATCTCTCGCTGCTGAACTACCAGGTTAGACTTCTTTCTGCAAGTCATGAGGCTTTCATAAAC
TTTTCCTGAAGGCTTTCCGTAGAATGTACAATTCCCCTCTGGGTCCAGGCATGGGCGCCCGGGT
AGCACATCCACTTCTTATCACCCCTGAACACCTTAGAGCCCATCAGCTTATCAAACCAGCAGC
TGATGTGAGTGCAGAGCAGACTGTGAGAGGTGGAGGCTGATACCAGTGAGGATGCTCCAAGC
TGGGACCCAGCCCTGAAGCGGGAGCCCAGATAATGGATGGGTGGAAATGGGCCTGGAGCCCA
GGAGAAGTGGGAGGATGAGGGGGCAGGGGGAGGAGAAGCCTGAAATCAAATGTTATTTCCT
GACCAGTTTGGGGTGCATGAGCTCTGTCAACAGCTCATGGAAACTGCTGCCCTAATTTCATCT
TGTTGGCTGAGGCACAATTCCTCTCTCAGGGACAGTGTAGAGCCTTGGGGAGGAAGGCCCTG
AGCGCGTATACCTGGAATCAGGGAATCGGGATCAGGGGCAGCAGCTGTGCCCAATAAAGCCC
CCACCCAGGATCCTCTGACTTCCTCATCTCTTTTTTTTTTTTTTTGAGCTGCAGTCTCACTCTGT
CATCCAGGCTGGAGTACAGTGGTGCGATCTCGGCTCACTGCAACCTCAGCCTTCTGGGTTCAA
GCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCGCCACCATGCCAGGC
TAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCT
GACTTCAAGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTAGGATTACAGACATAAGCCACTG
TGCCTGGCCTTTTTTTTTTTTTTTTTTTTGTAAACAGGGTCTCCCTCTGTCACCCAGGCTGCTGG
AGTGTAGTGGTGTGACCGCAGCTCACTGCAGCCTTAACCTTCTAGGCACAAGCCATCCTCCTA
CCTCACCCTCCTGAGTAGCTGGGACTACAGGCACTCGCCACCACGCCCAAGTAATTTTGTATT
TTTTGTAGAGACAAGGTCTTGCTATGTTGCCTAGGCTGGTCTTGAACTCCTCAGCTCAAGCAAT
CCTCCCTCCTTGGCCTCCCAAAGTGCTGGGATTGTGCTGGGATTACAGGTGTGAGCCACCATA
CCTGGTCTGACTTCCTAATCTTTAGGGCCCCAACTCTGCCCTTATCCAGGCAACTCTCCTCTCC
CCATCTTCCACTAACTTCTTTGGAATATTCCAGAGCTGTAAAAGCCTTAGAGAGTATCAAGTCC
AACTCCTATGTGTTACAGACAGGGAAACTGAGGCCTAAAGAGGGTAATGGACTTGCCTAAGAT
CACTTAGTGAGGTGAGAGAAGAAAGAGCTAGAGACAGCCTAGCCTGTGCAAGGACATAGTTC
CAGGCATTCAGAGCTGGGCTCTGCTGCCGGCATGTTTGGGGCCTGGTAGTTAGTTCACTGCTG
AACTACCAGGTTAGATTTTCTTTCTCCAAGTTGTGGAGCTTTCATAAACTTTTCCTGAAGGTCT
TCCTTACAATGTACAATTCTCCTCTGGGCCCGGTCATGAGCGCCCCTCACAGGCTCTCTCTGGT
CCCCTTCTGTAAAATGAGAGGAAAATGGAAGAATTGCTCTACTCATGGAATCTTCAATAAGTCT
GGGCCCTATGCATATAGCATTGCTACAAAATGGCAGATGCACTTTAACAATCGTGTTTAATAAA
AGGTTGGATTTGCATATCTGAAGTGGGGCATGCAGTCTCCAACTGAACACAAGCCTCACTGCT
CCCGCATGTGCACTGCACCTTCATATACATATTTCCTGCTTGGCTCCTGAGGGAATTTGAGTAAT
CCCAAGAGGAACCCCTGTAGAAAATGTCCCCTGGCCACACACCCCCATTCCTAAGGATGCAA
GCAGGAGATAGAAACATTCCCTGCACCTCCCTCCTTGTTGTCAGAAGAAGTGCAAAGAGTTG
AATCCTTCCTAATGCCCACTTCTCACCCACGCCCCAAATCCCCAGGTCCCATGGAGGTCCTTGG
GGGCCTCCTATATCCTGGTGGTGTCAGGTTGATTTGGAAATGTCAGTGTCCTCCCTTGTCCTCT
CTGGCAGACCCTGGGTATGTGTATGTTTCAATGGAAGTGAATTTAAATGTACTTTATAAATCAA
AGACTTTTTCTGAGACTTTGGAGAGTTCCAGTAATGAGAGCTTCTCATTGTTATCAAGGCCAG
GGCTGGAGACCAGTGGCAGGTGAGTTCCTATTGCTGTGATTGTCATGATGATGTTGATGAACA
GTCACTATTTATTGAGCGTTCTCCATGTGCCAGTCACTGTACTAAACATTATTTCCTTTGGATTT
CCCAGAAACCTCTCAGGTGGGTCTAATTACCCTTATTCAGCTGATAAGGAAAGTAAGCAACTT
ACAAGACCACAGGGCTATGAAGTGGAAACACATAAATTGATATTTCATTTTATTTATTTATTTAT
TTTGAGACAGAGTCTCACTGTGTCGCCCAGGCTGGAGTGCAGTGGTGCGGTCTCAGCTCACT
GCAACCTCTGCCTCCCGGGTTCAAGCGATTCTCCTGCCTGCCTCCCGAGTAGCTGGGATTACA
GGTGCCCACCACCACATCCAGCTAATTTTTTTGTAATTTTAGTAGAGACGGGGTTTCACCATGT
TGGCCAGGCTAGTCTCGAACTGCTGACTTCATGATCTGCCCACCTCATCCTCCTAAATTGGTAT
CTTTATATGTCCAAAAGAGTCAACTGGTGGCAATTTAGTGAGGTTTAATCTAATAGGAAATGAT
AGAGCTGGGATCGAACAGAGCCATGTGAACTCAAAACCTATGCTTCCCCTTCCACCTTTTTGA
AAAACATTGTCTAGGCTGGGCACGATGGCTCATGCCTGTAATCCCAGCACTTTGGGAGACGGA
GGTGGGTGGATTACATGAGGTCAGGAGTTCGAGACCAGCTTGGCCAAAAATTAGCCAGGCGT
GGTGGCGCGCGCCTGTGGTTCCCACTGAAGCACAGGAGGCTGAAGCACAAGAATCACTTGA
ACCCGGGAGGTGGAGGTTGCAGCGAGCCGAGATCGCACCACTGCACTCCAACCTGGGCAAC
AGAGAGACTCTGTCTCGAAAAAAAAAAATTGTCTACATGCTGGTTGCAGAAAATTTAAACACT
AAAACTAAAAAAGTAAAACACCTCCCAAACTTAGAGACAATATTAATGACGGAAAAAAAATT
CTTCAAGATCTCTCTCTCTCCAGTCATTTATTCATGTGCGAAAACAGTTGGTGATTATTGATAAA
ATAGCTTTTAGAGTTTGGAGCAATTATGTGCATTACATATACCATTTGATTCTGGCAACCTAATG
AAGGAGTATGATCATTTCCCCTATTTAACAGACAAGAACAAGAAGAGGGAGGGCAGATGGTG
TGGTAGTCTAAGGCACAGGCTCCAGCAGATTATCTAGGTGTAAATCTTGGCTGTAGGCCAGGC
CCTGTGGCTCATGTCTGTAATCCCATCACTTTGGGAAACCGAGGTGGGCAGATCACTTGAGGT
CAGGAGTTCGAGACCAGCTTGGCCAACATAGCGAAACCCCTTCTCTATTAAAAATACAAAAAT
TAGCCGGGCACGGTGGCAGGCACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAAT
CACTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCCAAGATCTTGCCACTGTACTCCAGCCTG
GGTGACGAGTGAAACTCTATCTCGATATTAAAAAAAAAAATCTTAGCTCTACCCACCGGGGCA
AGTTACGTAACGCCTCTGTGCCTTGGTTTTCATATCTGTAAAATGGTGACAGTAACAGCACCCA
CGTCAAAGTGTGGTTGTGAGAACGAAACAAGATAGTCTATGTAAAGTGATTAAAACAGCGTA
GGCACATGGTAAACGCTTAGGAAATGTAGGCTGTTATAAAGCTCAGAGATGTTAAGTAACTAG
ATCAAGATCACACAGTTAGAGGGTGCCAGAGTCCTGATTTGAACCCAAGTTTGTCTCGTTCTG
GAGCTCAAGCTGCTAACCCTTTTTCAAAACTGGAATTAAACCAAAGTGCTCACCCTCCGCTTT
GCTGGGCCCCTCCCTGCCCTCAGGTGCGTCTCTTCCACTCACCTGCCACAGCAGCCTCTGCTC
AGGGTCTGAGACCGGGAAAGGTGAGGGCTACCCAGGTGGCCCTGATGTTTTCTGCCAGCCAG
CTCACCAGGTCCCTCGCAGCAGGCGGCAAAGGGAGGGAGGTTTGCTGTGAAGATTATGTGGT
TCCCAACAACAAGAGCGCTGGGCCTATCTCTGCCCTCTCTTTTCTGTGTGTCCTGGGACAAGT
CACTTGGCTTCTGTGGCTTCATTTTCTCATGTGCCCAGCCAGGGGGTTGGCCCTCATATGCAAT
AACAGCAGCAATGACCTTTACTGAGTGTCCATGTGCGTCAAGCACGTGTGCTTTACACTTGTT
CTTATTATTAGGTTTAATAATAGAATAATTGCCACATTTACTGAGCACTCATTATGGGCCAGGCC
CTGCCCTAAGTGCTTAATTAGCTTTAGCTCCTCTAATCCTTATCTTATCCCCACACGGCATGTTAT
GTTATCCCCATTATTCAGTTGAGAACATTGAGGCTCAAAGAGGCAAAGTAACTTGACCAAATA
CTTGTAAACGATCTTGCATGCCCCTTCCAGCTGCCATTTAGTAAGACTCTAATTTCATACCACCC
TAAATCTCGTCTGCTTCCCCCTCCTCCTTCTCGCCATCTCCCCACCGAGCAG
The "TTGGCCAAGATCTGACCGTGATGGCGGCCATTGGCTTGGGCTTCCTCACCTCGAGTTTCCGG AGACACAGCTGGAGCAGTGTGGCCTTCAACCTCTTCATGCTGGCGCTTGGTGTGCAGTGGGC AATCCTGCTGGACGGCTTCCTGAGCCAGTTCCCTTCTGGGAAGGTGGTCATCACACTGTTCAG" sequence segment is: rhdexon2
GTATTGGGATGGTGGCTGGATCACTTCTGGGTCATAGAGGGAATGGACCCCGAAAGGACAGG
TTCCAGAAGATCTGGGATATTGCCCCCTCTCTGTCTAGCACCAGTGCTGTGCAATATTTAGGAC
ATCCTTATACTAAAAGATTATTCATTGTTTAAAATTCAAATTAACTGGGCATCCTGTATTTTACTG
GACAGCCCTACTCCGTGTATCACAAGGAATCCAGGCCTACATTCCTCCTGCATCCTTTCTTTCC
TGTTATTGTCGATTATGATTTTGTAAAGTTACATAATCAATATAAGTTTATGGAAAACGTAAGAA
GGAAACACGTTAGACAGAGAGAAATAGACATGCCACACCTAGAGAGACATTCTATTTTTTTTT
TTTTTTTTGAGACGGAGTTTCACTTTTGTTGCCCAGGCTGGAGTGCAATGGCGCTATCTCGGCA
CACCACAACCTCAGCCTTCTGGGTTCAAGCGATTCTCCTGCCTCAGCCGCCTGAGTAGCTGGG
ATTACAGGCATGTGCCACCGCGCCTGGCTGATTTTGTATTTTTAGTAGAGATAGGGTTTCTCCG
TGTTGGTCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCGCCCGCCTCGGCCTCCCAAAG
TGCTGGGATTACAGACATGAGCCACCGCGTCCAGCCTGAGAGACATTCTCTTGAAAAGAAAG
GACTTTCAGCCCCCTAATGCTGCTAGACAATAAATAGCCATGCCTTTATTTTCATTAAATTACCT
GTGCTTTGTTTACATGCATTTGTGTGAAATGCTAAGAACCATCACAACTAATGTATGGTGCCAG
AAGTCAGAATAGTTGTTACCTGGGCAGGAGGTGGATATTGATTAGGAAGGAACACAAAATAAC
CGCATGGGGTGCAGAAAATGTTCTCTATGTTCACCTGGGTGATGATTACACATCAAGCTATACA
CGTTTTAAAAGGGCATTGGCACTTAATAGGAGGAAGTAGGCTAAATTTTTTCCTGAAACATTGT
TTTGTTTTGTTCAAACCTCTGAATCCCTGTGCTGCCCAGATGATGGTAAACGTCATCCTAGGCA
TCTTAGGGACCTCTCAAGGCCATTCCAGCCTCCCCTTCTAAGACCCTGCTAAACCTCTGGGCA
CTGCTGTTAAACATTTCTCTATGAGCCAGGAACTGTGCTGAGCACTCCACAAATATTATTTTGT
TTAACTCTTCCGGGTAGGGATCTAACCTGGTATACAGGTAAGGAAGTGGAAGCTCAGAGAGG
GCAAGGCACTTGCCTAGGGCCACACAGCTAAGTGGTGGAGATGGCTCCAACTTTTTATTATAA
CCTTTTCCACATGCTCCAGAGTGCTCAGAACATGAAACACAGTCTAGCCAGCTCCCGATTGGC
CCTGGAGGGAAAAAACTTTATATATTTTTCTTTTTTAAAAGGTTTAGAGGCTGGGCATGGTGGT
TCACACCTGTAATCCCAGTACTTTTGGGAACCGAGGTGGGCAGATCACTTGAGCCCAGAAGTT
TAAGACCAGCCTGACTAACACAGTGAGATCCTGTCTCTGCAGAAAATAGAAAAATCAGCTAG
GCGTGGTGGTGTGCACCCACAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGGATCACCTGA
ACCCAGTGAGGTTGAGGCTGAGTGAGCCATGATCGTGCCACTTCACTCCAGCCTGGACAACA
GAGTGAGACCCTGTCTCAAAAAACAGTTTTAGGGGCCGGGCGCAGTGGTTCATGCCTGTAAT
CCCAGCACTTTGGGAGGCCAAGGCGGGGGGATCATGAGGTCAGGAGATCGAGACCATCCTGG
CTAACTCGGAGAAACCCTGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTGGTGGTGGG
CGCCTGTAGTCCCAGCCACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCG
GAGTTTGCAGTGAACCGAGATGGTGCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCC
GTCTCAAAAAAAAAAAACAAAAACAGTTTTAGGCCAGGCGCGGTGGTTCATGCCTGTAATCC
TAGTACTTTAGGAGGCCTAGCAGGTGGATTACCTGAGGTCAGGAGTCCGAGACCAACCTGAG
CAACATGGTGAAATCCTGTCTCTACTAAAAACACAAAAATTAGCTGGGTGTGGCGGCAGGCA
CCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGCGAATCACTTGAACCCGGGAGGCGGAG
GCTATAGTGAGCCGAGATCGCACCATTGCACTGTAGCCTGGGCGACAGAGTGAGGCTCTGTCT
CAAAAACAAAACAAAACAAAAACAGTCTATGAGTTAATTCCCACCAGAATTCAATACACACA
CGCACACATGCACGCATACACACACTGTGTCCACCTGGGAAGTGACAAAGGGCACCCTGGGG
GATTTCAAATGGTGGTGGCCCTGGTTTGGTGTTGCTGCCTTAGCTTAAGGTCACACCAGCCTT
CAGCCTCCTGCCCCACAGTCTAGGGCTGCTCCCCTCATCTGATGTCCACAGGGACCTGTTTGT
TCTTGACTCAATCTAGAAAGACGAGAAGGGAGAGAAGTCACTCGCAGCCTGAGTGAACTCCC
CTGCCCCACCCCTGACTGCTTGGATCCCCCTAGGGGTGACCCCTGCTGAAACTGGCTCCTTCC
TGACCGGTTCCCGTCAGGGCTGTGCTGATGGGTGGTGCCCAGGCCTGCCCCTGGGGACGGGG
TACTCTCCCTTGGCAACACTCCAGCTTGTGCCACTTGACTTGGGACTGATTTGGTTCTGTTTTG
AGTCCCTTCAGGGGAGGGGCCTATCTTATTCAACGTTGTTGTTTGTTTTCCTCACATACTGATA
ACTTAGCAAATGGCTATTGGAGCAAAAATGAAAATAAACGGAACTCTGAAGTGGGATGTTTTA
AAATTTTATTTATTTTTTTAGAGATAGGGTCTTGCTCTGTTGCCCAGTCTGGAGTGCAGTGGTAC
AATCATAGCTCATTGCAGCCTGTGCCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCCTCCTGA
GTTAAATTTTTTTACAGGCGCCTGCTACCATGCCCTGCTAATTTTTGTATTTTTAGTAGACAAGG
GGTTTCACCAGGTGGGTCAGGTTGGTCTGGAACTCCCGACCTCAAGTGATCCACCTGCCTAGG
CCTCCCAAAGTACTGGGATTACAGGCGTGAGCCACTGTGTCCAGCCTAAAACTGTTTTTGAGA
CAGGGTCTCACTCTGTTGTCCAGGCTGGAGTGAAGTGGCATGTTCATGGCTCACTCAGCCTCA
ACCTCACTGGGTTCAGGTGATCCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTGTGGGTGCA
CACCACCACGCCTAGCTGATTTTTCTATTTTCTGCAGAGACAGGACCTCACTGTGTTGCTCAG
GCTGGTCTCAAACTCCTGGGCTCAAGTGATCTGCCCACCTCGGCTCTGAAAAGTACTGGAATT
ACAGCCTCCTGAGTAGCTGAGACCACAGGCACACACCACCACACCTAGCTTTTTTTTTTTTTG
CTTTTTGTAGAGATGGAGTCTCACTATGTTGCCCAGGCTGGTCTCAAACTCCAGGCCTTAAGC
AATCCTCCCACCTCAGCCTCCCAAAGTGCGAAGATTACAGGTGTGAGCCACCATTCCTGGCCT
TAAAAGTGTGATATTTTTAATGTATTTTGAAATCTGCAGGACTCTCCCTAGAAGATAATAGCAAT
AACCAACTCCTTTATTGTGCTTGACGTATATCAACTCACTTTGCCCTTACCGTGGCTCCAGAGG
CATTGGGTCCACCTTATAAATGGAGGCACCAAGGCACAGAGTGATTAAATAAATTGCCCAGGA
TCACACAGCCAGAAAGTGTCTGAGTCAAGATTCCAGCCCAGGCAGCCTAGACCTGAGAGCAC
GCTCCTAACCACTGCACATCACTGTCTTAGCACCTCCTCAGCACAAACTGGCCCTTGAGGAAT
GAAATACCGCCGCCGGCACACACGCTCCTGAGTTAAGCCTTTGTCAATGAAATGAACACCCAC
TTAAAAGGAATAACCTGTCCAGGCACGATGGAACATTGAGTAACCCCTTATTCTAAATTCCTGG
TCCCTGTAAGACTCCTTCCCCATGCCCTTGCCCTTTTCTGACCTTCCCCTAAAGTCCTTGAGGC
TTAAGCGGGCATAGTCTGCAGCAAACACTGGGGAAGCTGAGTCCAGACTTCAGAGCACAGGC
TTTGGATCTAGGCCAGCTGGATTTGAACCTCACATTTGTGATCAGCTGGCATGACTGTTTCCAA
AAAGTCCATTTTAATCCTCTACGTGACCCTCTGTAAAATGGGATACTGAATGGTGAGCTAGCAC
GATTTTACAGAGAGTGAATTTTTTTTGTGTGTGTGTGAGGCAGTCTTACTCTGTTGCCCAGGCT
GGAGTGCAGTGGTGCAGTCTCGGCCCACTGAAACCTCTGCCTCCCGGGTTCAAGCGACTGCC
ATGCCTCAGCCTCGAGAGTGGCTGGGATTACAAGCATGCACCACCATGCCCGGGTAATTTTTG
TATTTTTAGTTGAGACAGAGTTTCACCATGTTGGCCAGGCCACTCTTGAACCCCTGGCCTCAA
GTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGAGTACAGGCATGAGCCACTGCACCCAG
CCTTATAGGGTTAAAATTTAAAAGAGGTGATGCTGTTACAAGCCTGTTTTACAAAATGCTCTTA
TAATAAATCATTATCATCACTGTTGCTGTGGTTGTAGCATCATCATCATTAACTCCCAGAGGGAG
GAGGGAGTCTCAGAGCAAGCTGCTCAGGGGAGACTGGATGTCCATGGATTGTCCAGCTCAGT
ACCACTTCCTCCAGGAAGTCCTCCCTGATAAGTCCAGTCAGCATCACCCTCTCCTTCCAATGA
ACCCCACTAGCCTTGTGATATCACAGATATTCTTAGTTGACAGGCTCATGGTGTAGCCTGTCTA
GATCATAAGTACATTTTTTTTTTTTTTGGATCATAAGTATCTTCAAGACCAAAATAATTTTCTACT
CCTGAGCATGCTCATTGGTCAAAGGAAGGAAGGAATCATAATAGCGTTAATAAGGCTAGCGTC
TTTTCAGAAGTTGGTTCTTTGTGCCAGTCTTGGTGCTAGACACACCGATAGGAAGAATACTCC
TTCACATCCCCAGGACACCAACATGGGATACGTTTGATCATCATTCTTAATTTGCAGAAGGAGA
AATAGGCTCAGTGAGATGAAATAGCCACTCCAGTGGCAAGGCTGGGACTGGAAGCCGGGCTT
GTCCTGATTCCAAATCCAGTTTCTTTCCACTGCCACGGAGACGGAGAGAAGGGACAGTGGCC
CCAGATGGGGATGGGGTGACTGGATGTGGGCAGGCCTGCGGGGGAAGAGTGCCCTCTGTTGA
GCATCCGAATGATGGCAGCAGAAAAGAAGACTGGGCAGAATCCCAGTTATCAGATCCCCTGA
GGGAACAGTCACCCCGATCACCCTCAGTCAGATGAGTGTGTGTAGATCAATGCCTCATAGATG
AAGGCACTGAGGCACAGAGTGGTTAAGTCATCTGCCAGACCACATGGCTCAGGGTGCAGAGG
CCACCTTAACGGGAGAAGAGATGGTCACTCCACTCTGCAGCATCAGCGCCCAGGTGGGTAGA
AATCTTGTCTTCTATTCCCACAGAAAGTAGGTGCCCAACAGTGTTTGTTGAAAGAATGAATGA
ATGAATGAATGAATGAATGAATGAGTGAGAGGCATCCTTCCTTCTCAGTCGTCCTGGCTCTCCC
TCTCTCCCCCAG
The "TATTCGGCTGGCCACCATGAGTGCTTTGTCGGTGCTGATCTCAGTGGATGCTGTCTTGGGGA AGGTCAACTTGGCGCAGTTGGTGGTGATGGTGCTGGTGGAGGTGACAGCTTTAGGCAACCTG AGGATGGTCATCAGTAATATCTTCAAC" sequence segment is: rhdexon3GTGAGTCATGGTGCTGGGAGGAGGGACCTGGGAGAAAAGGGCCAAAAGCTCCATTTGGTGGGGTTTCCAGGGTTTTGAAAAATAAAGACAACCTGTAATCCCAGCTACTTGGGAGGCTGAGGAAGGAAGATCACTTGAGGCCAGGAGTTTGAGACCAGCCTGGGCATCATAGCAAGATCCTCATCTCTAAAAAGTAATTTTTTCTAAATTATCCAGTTGTGGTGGCATGCACCTGTAGTCTCAGTTACTCAGGAGGCTGAGGTGTGAGTTGGAAGGATTGTTTGAGCCCAGGAGTTAGGGACCGAGCTGGGCAACATAGCAAGACCTCATCTCTAAATAAATAGGTAGGTGGATAGACAGATAGATAGATAGACAGACAGACAGACAGACAGACAGGCTGGGTACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGAGGGCAGATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGGTCAACATGGGGGAACCTCATCTCTACTAAAAATACAAAATTTAGCTGGGCATGGTGGCAGGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAAGAGAATCGCTTGAACCCGAGAGGTGGAGGTTGCAGTGAACCGAGATCGCGCCATTGCACTGCAGCCTGGGGGACAAGAGCAAGACTTCATCTCAAATTTAAAATAAAGAAAAAAGAAAAGAAAAGATTGATAGATAGATAGATATCCAAATGAGTTTACAAAAATGTGGTCTGTGCAAATGTTTAAACACAACAAACCAATGCCTTTAACTACTACAGTATAATCCTGTAGGATTGTGCTATTCATGATATAATTATGGTTATATAAAAGTAATTAATTCTCAGAGCCTCACCAGCAGTGGGTCCAGCAAGTTTGTACAGCCAGCATCTTCTTTCAGTCAGTGCGTGTCAGTAACTGCATATGTCCTCTCATTGGGAGAGCCTGTCGAAAGTCTAAATTTGAAGGCAGCTGTGAAGGTAAGGCCAATCCAAATGGCTCTCCCAGATCCTCTGCTGTAACCCTGACCCTGAGTGAGGACATAGCCAACCTTCCCATCTCATAGGTGAGAAAGCTGATGCCTGGAGAGGGGAAGGGACTGCCCAAGATCACATAGCAAGATAGTGGCAGAACCCAAGCGAGAACCCACAGTTCCAGCCTGGCTTAGAAGAAAGTGCACTGGACTTGGAGTCAAAGGCTGGGGTTTGCATCCCAGCTCTGCCATAAATCCCTGTGTGACTCTGGGCAATTTAACCTCTTAGAGCTTTAGTTTCTTCATCTGTAATATGAGGGTAGCAGTACTACCACATAGGGTTTTGAGGGAGTAATTGAATTAATCACATGAGATGATGCATGTTTACAAAAAAAAGCATGAAGCCCCTTTACTGTGCCTCAGTGTCCCAAAGGACTTTGGATTTTACTCTGAGAAATACAGGGAGAACTAGGGAGTGTTGGGCAGAGGAGAGCCATGATCTGACTTATGTTTTAAGATACTCTGGCTTCTGGGTTCAGAAAAGACTGAAGGGGCAAGAGAGGAAGCAGGTGGAGACCAGAGCGGCAGTGATTGCCATCATCCAGACTCAGACTAGGACAATAGCTGTGAGAGTGATGGGAAGTGGTTGGATCCTGACTGTATTTTAATAGCAGAATTGACAGGATTTGCTGATAGACTGCACGTGGGGTGGGAGAGGGTCAAGATGACTTCAAGGTTCTCATCTGGCACAACTCAGCGGCTGCTGGTGCCATTTACTGAGATGGGGAATGTTGGGGTGGGATAGATCTGGGAGGGAAAACCCAGAGTTCAGTGTCGAATGTGGTAGCGTTAGGGTTAAGGTTGGGGGAGGGGGGGTAGAGATGTGTATGAAACATCCCAGTGGAGACACTGAATGGAGATGTACAAGTCTGAAGCTTAGTGGAAAGGTTAGGGCTAGGGATATAAATTTGGGAGTTGTTACAATACAGATGGTGTTTAAAGCCATGAGACCCAAGGAGATCACTCAGGAGTGAGGATAAAGAGAGATGGGAAGAAGTCTGAGGACTGAGTCCTAGAACACCCTGCATTTTAGAGGGGGGACATGTGTAAGAGCCAGCAAAGGAGACAGAATTGTGCTTGGAGAGGCAGGAGGAAGCCCAGGAGAGCATGAGGTCCTGGAAGGCAAGGAAAGAGAGGGCCCCAGGTGGGCTGAATGCTGCTGAGAGGTCAAGTCGGATGAGGGCTGGGAAGTAGCCATTGGATTTGGCCAGGAGACCTTGGCATGCATGGTTGTAGAGGAGGATGAAGGCAACAGCCTGGCTTGACTGATTCAAGAGCAGGAGATGAGAAAGTGGAGACAGCATGCAGGGGCAGCTCTGCCAAGGACTTTGCTATAAAGGGGAACAGAGAAATGGAGGAGAAGCAGGAGGGCAATAATCCGATAGAGAGGAAAAATCTGATGATACAGAAGAGAGATGAACTGCAAGAGTCAAGCCTTTGAGTTGGAAAGCAGGAGTGGGATTTTGAGCACTGATACCTTTAGGCCGATGCAGGGACAGTTCATCTTTTTTTTTTTTTTATACAACATTTTATTTAAAAAAATTATTTTCATAGAATACATTTTCACATTAGAGATTCCCATTGTGCGGAAATAACAATTTATTACTTATAGTTTTATATTTGTGGACAGATTGTTTTAGAACAAGTAGAATACATTTGAGAATTAAATCTCAGTTTACAATGGATAATATTTTGATATGTCTCTGGGGAAACTTGCCCTTAAATGGAACTTCTGTATCTTCAGAAGCACTCCAAGCGTTTCTTCCTAGGATTTAGAAATTTATAATATGAGATAGCAGCATTTCCTAATTTTAAAATTTCCCTAGTATATGTAACCATCAGTAGGTGGTATCTACTGACTAGAGAGGGAAGTTTTTGAAAATTAAACACTGTCTAATTTTCTGCAAAGTTTTTATTCATGAATTAAGAGTATTTCCCTTTGTCCATTATTCCCAAGGCAAATATGGAAATTTGATCATGTACTAATCATAATAAAGCTGGATTCTCTTTAAGAGATTGAGAAATTAAAAGGCAAAAGCTGATATATCATGTTTAGTTATATTGTGAGTCTTATAAGAAGCTGGGAGGCAACCCCATTAACTCACCAGAATACAGAACTCAGTCTCACAACTTAGATATAATTCCTCTCAAACCTTTTCCTCAAAGATTAAATTCTGAAAATAATCTTGTGATTAAGAGAAGAAGGCTGTCCACCAATGGGCTTATCTGTTATTTCTTCCTTATTGTGAGCTTAATGGCATGACAAAGCAGAGGCAAAGAGGCATACATCAATTCTTCAAAGTAGGAAGTCAAAAAGGTCAGAGCTTCCACAGCATGGCAACAGCTTTGCAGATGCCCACATCGTGATAGTTGAAATAGCAAAGCCCAGCAAAGGTTAAAGCTGAAAATGCCAAAAGCCCTGCCTTGGCAGCTTTCTGCGAGGCATCCCCATGAACATAATCAGTAACAACTTGTCCAAGGCCCCAGTGACCATGAAGAGTGAGGGCTGCAGCCAGGGAATAGTCCGTCGCAGAGCAAGGATTCAAATAAGCAGCCGGAAGCAGACCCGGGAGCAAAACACTGACAACCCTCTCGCTAGTCCAGTGGAGAGATGCAGCCTTGGAGCCAGAATGGTGGCTCGGTGACAAGTGTATGTGCTGCACTCCACACCATTCTGGGATAGGTCGGTCCTGAAGAAATGCTGAGATATGAGCAGGTCTGACCACTGGAGTTCGCAGCAACAGAGCTCGGCCTCCTTGGGCACCGCAAACGGCACTCAGCCTCCAGGGAACCGCCATCTCGTTCCTGAGGCGGAGAGTTCATCTTAACGAGAGAAATGGCAGGGACTGTGAATAGGCCGGCAGATTTGGTGGCGGGTGCCACAGGTTCAGTCTCCTGCAGGGAGAGGAGAAAATGCCTTACTAATTCCTTGTATTTTCTCAGAGAAACAAGAGGCACCGTCATCAGCCTCATGTGAGGGTGGGAAGGAGGGATGGGGTTTGCGGAGAGGGAAAGTGTGGTATGGTCATCTGTGGGAGTGGAAGAGAGTGAGAGGGCTGCAGGGGTGCAGCGGGACTGCAGGCTGGCACCAGGGTCCCTAGGGCTTGTAGTTGGTGGAAAGTGCATCAGTGACCAGGGCTGTGTGCAGCTGCTCCAGGCAGGTGTGGAAGAAGCAGAGTTGAACTTGCCCAGCCTGGAGTGCTGCCCAGAGTGAGCCCAAAGCCCAGGGGAGACCAGAGATGGGGCTGTTTGCAAAGGAGGAAGTATAACAGTAGCCCACAAAATCTGAGCTGGTTAAGAAAGGAGAGAGAGTGAAAATGGGGAGCCCAGCCTGGCAGCCTGGGTACACATCTCAGCTCAACCCACACTAGCTGAATCCATTTGGGCCCCTTCGTTGACCTCTCTGTGCCTCAGTTTCCCTATCTATAGAATGGGGATAAGAATAAGGCTACTTCCTAGGGCTGTTGTGAGAATTGAACAAGTGACCGAACACTTGTTCAATTTTGAACACTGTTCTAAAGCATTTAGGACAGTGCCTGGCATGGGGTAAGTGTTGCGGCAGTGCTGTTATTTTCATCATCACCATTGTTCTCAGGCTGCGTTGATTGGAGCTGCTGAAGGGAGGCAATTTAAGGAAGTGAGCCGGACAGATAGGAGGTGGTGGTGGTTATCAGGTGCGATGCTTGAAACTGAGGCTTCGGAGGCAACAGTTACTGGTAATGACAAGGTCTAAGGCTTGACAGTGGGTGGCAGAAGTGTAACGCAGGGAAAGAGACGAGCGGTCAAGGAGCCGAGAGGGAAGGAGTTGGGTGGACTAAGATCATTTGTGGAAGAATGATGGAGAGAAAGGCTGAAGGGCAGGGGCTGACATCATCAGTGACCAAGAGGCGGCCGGGAGGCTGAGACCACAGCAAGAAAGGGAGAGTGTGATGGCATCTTCTTCAAGGGAGCAGGAGATGTTTGGGGTGGAAAAAAGAACAATGGTCTGGGAGGGAATATGGGAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGATGGAGTTTCGCTGTTGTCATCCAGGCTGGATTGCAATGTTGCAATCTTGGCTCACTGCAACTTCTGCCTTCCAGGTTCAAGTGATTCTCCTGTCTCAGCTTCCCGAGTAGCTGAGATTACAGGCACACACCACCACGCCTGGCTTACTTTTGTATTTTTAGTAGAGACGGAGTTTTGCCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATTAGAGGTGTGAGCCACCGCGCCCAGCCTGGAAGTTTGTATTTATTAATTTTTGGTTGTCTTCATCTGTGTATGTGACTTTAACCCCTAAATACTTCAGTGTACATTTCTTTTTTTTTTTTTCTTTGAGACAGAGTCTTGCTCCATCAATCACCCAGGCTGGAGTGCGGTGGTGTGATCTCGGCTCACTGCAACCTCCGCCTCCTGGATTCAAGCAATTCTTGTGCCTCACCCTCCCGAGTAGCTGGGATTAGGGGCATGCCACCATGCCCAGTTAATTTTTGTATTTTTAGTAGAGATGGAGTTTCACCATATTGGCCAGGCTGGTCTTGAGCTCCTGGCCTCAGTTGATCCACCTGTCTCAGCCTCCCAAATTGCTGAGATTACAGGCGTGGGCCACCATAACCGGCCTCAGTGTATATTTCTGATGCAGTTGGGTTCTGTATCCCCCTCCAATCTCATCTCGAATTGTAATCCCCACGTGTTGAGGGCATGACCTCGTGGGAGGTGATTGGATCACAGGGGTGGTTTCCCCCATGCTGTTCTTGTGACAGTGAGTGGGTTTTCAGGAGAGCTGATGGTTTGAAAGTGTGGCACTTCCTCTCTCTCTTTCTCTCTCTCTCTCACCTGCCACCACGTAAGATGTGCCTTGCTTCCCTTTCACCTTCCACCATGATTGTAAGTTTCCTGAGGCCTCCCCGGCCATGCCAAACTGTGAGTCAATTCAGCCTCTTTTGTTTATAAATTACGCAGTCTCAGGAAGTATCTTTATAGCAGTGTGAAAACAGACTAACACAATTTCCTAAAACAAGGGGACATTCTCTTACATAACCTTTTTTCAGTTAACAAAAATGAGAAATTGACATTGATATATTATGATTACCTTATTCTCATTTCACCAATTTTCTCAATAATATCTTTTCTAGAAAAAAATATATATTTTTTGTGGTCGAGGATTACATCTTGCATTTAGTTCTCATGTCTTATTAAATTCCATCAATCTGGAACAGTTTCTTCATCTTTCTTTATCTTTCATGACCTTGACATGTTTTGAAGTTTCGAGCCAGTTCTTTTGTAGAATGTGGGTTTGTCTGCTGTTCCTCATGATTAGATTGTGGGTATGCATTTTTGGTAGGAATTCTCCAAGAGCCGTGTGTGCCCTTCTTAGTATATCATATCAGAAGACATGCTATCAATTTGCCCCATTACTGGGTGTGTTAACTGTGATCATTGGGTTAAGATGGTACCTGCCAGGATCTTCCACTGCAAAGTTACTATTTTCCCCTTTGTAATTAATAAACATCTTGTGAGGAGATAATTTCCTATAGAAATCCTGTTGATCGTCCAACTTTCACCCACTGATTTTAGTGTTCATTGATTCTTCCCTGAATAAATTAGTACTATAATAATTGCCAATGGTGGTTTTCTAATTCCATCTTTCCTTCAGTAGTTGGCATTCTTCTGTAAGGAAAAGCTTTCGCTTCTCTGTTCATCCACTCATCTATGTACTTATTTATATCACCATGGGCTCCTGGATTCCGGTTTACACACTTCCATTTTCTGCCTTTTCTCTCTGCTTAATATAAGGATTAATGAGAACTCCCTGATTCCCAGGAAGAAAATGTCAGCAGAGCTTTCTTAGGCGGAATGAAGAGAATTCAGTGTAAGAACCATAAAGGTGTATCTGTGTAGTATGGACAGTTTTAAAAAACAAACAAACACAAAGAACCTCCAAGGGCAGGAGGTGCTGCCAGACTCAGGAGGGCACTAGAACTGGCTATGAGAAGCCACTGAGATCCCAGGTAGTCTGTGCTCTCCATCTTTTGGCTCTTATTCTCTCCGTACATCTAACATCTCTGTACACCAGCTTTCTCTTTAGCGAAAAACGTGTCCCCTCCACCCACCCATCCACCTCCACTTGTTCCTGCATTTCTATGTCCCAGATCCTGCAGAAAACAACTCTTTTCTCTCAGTTAGTCTCAATTCTGTAGTCCAGGGAGAGAGAATCTGATCAGTCCCCTGGGTCATTTTTCCACTCTGGTCCAAGCAGCTACAGCTGGCATGGGAAATAGTTCACACAGTAAAAACATGGCTGTCAAGAAGAGGAGTAAATTTCAGAGGCAGAACACTCCCTGTGAGCCCGAACCTCTTCCTGCTTTGTTGCAGTCTTCATAACGATTGCTTTAAAAGACTGCATTGATATAACATCATCTCTCTTCTCTGCATCTTTGACTTGCTAGCTTAACTGGTCTAGAGGAGGGCTTAGCACTGATTTTCAGTATTCATTTTCCTCAAAACTTCAATTCAGCCTGGGTTTCTTCAGCAGGAGGGCCCGGGGGAACCAGAGCCAGGGACCAGAGTCATTTCAGTGCACCAGCTCAAGAAATGAATATTCCAGGCCAAGAATCCCCAAGTGTTCTTCCTGAACTCCTTCCTGGTGGAGTTCAAAGAGATGAAAAACACAAGCCCGCTTTTCAGTTCTTATCAGGAAACTGCATAGACTTTCCTCTTTATGTATGACTGAGGGCTTTTTACCATCATTTGTTCCCTTCACAAATATTTATTTGGTATTTACTATATACCAGGGACTCTTGTGGCAGTGGAAAATACAACTCTCATGGAACGTCTGTTCCAGAAGGAAAGACTGCCAATAAACAATAAAATAGGCAAAAGATATAGCATGTTAGAGAGTGGTAAGTACCACAGATAAAAATGAAATGGAGAAAAGAAACACGAAAAGTTGGGGAGAGAGGATAACTGTTTGAGAGGGTGGCCAGGGGCAGCTTCATCTTATCAAGAGGGTGATTTTTTGAGTACAGACCTGAAGGTAACGAGTGCACAAGCCATATGGGTACCTGAGAACAGCGGCAGAACAATGGCAGGGTGCTGGGAGGGCTGTTTACCAGCCACGCTGTTTAGAATTGTCAGCACATGGTGATAAAAAAAAAAAAAAAAAAAAAAAAACAGGCTGGGAGCAGTGGCTCATGCCTGTAATCCCAGCGCTTTGGGAGGCCAAGGCGGATGGATCACTTGAGGTCAGGAGTTCGAGACCAGGCTGGGGAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCACGGTGGTGGGTGCCTGTAATCCCAGCTACTTGGGAGGCTGAAGCAGGAGAATCGCTTGAACCCAACGGGTGGAGGTTGCAGTGAGCCAAGATGGCACCAGTGCACTCTAGCCTGGCGACAGAGTGAGACTCCGTCTCAAAAATAAATAAATAAATAAATACAAATAAAAAGCAGACAGACTTTTTAGTTGGCTTTAGAATTCTTAGACACCCTCTACAGACAAGGCACCCCGATTGCTTGCACCCAGGGTGGACTACTCCCTCCACCCTGCCCTTGTTACACCCTGGCTGGGGGTCAGCATTTCAGGCAGCTGAATGACCCAAAGTGGGAACACGCTAGTGGGTTTGAGGATGAGCAAGTGGAGGAGGGCAATAGGAGGTGACGCCCGAGAGGTCAGGTGAGAGTGGATCCTGCAGGGTCGTGGCAAGAACCTGGACCTTGACTTTGAGTGACATGGGAGCCGCTGGAGGCTTCTGAGCAGAGGAGTAACATGATCTGACTTGCATTTTATTTTATTTATTTATTTGACGCAGTGTCACTCTGTCGCTGAAGCTGGAGTGCAGTGGCGACATCTCAGCTCACTATAGCCTCCGCCTCCCAGGTTCCAGTGAATCTCCTGCATCAGCCTCCCAGGTAGATAGGATTACAAGCAAGCATCACCACGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTTGCCATGTTGGCCAGGCTGGTATCGAACTCCTGACCTCAGGTGATCCACCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCAAAATTAGAATATATCTAGAATTTCCTGAAGACCTTAGTTTGGTATTATAAGAAGTCTGGTTGCTTCATGTTGCAAAATTTATATCACTCATCACTCCCGCAGAGTTAAAATTCCGCTGAGAAGTAGGAATCAGTGAGGTGCGTGTCCATGTGGGTTTTTGCCACACCTAAGTGAACCTTGGTCAAAAGCATATAAGAGCTACTGATAGGCCGGGTGTGGTGGCTCATGCCTGTAATCTCAGCACTTTGGGAGGGAAGGATCTCTTGAGCCCAGGAGTTCAAGACCAGCCTGAGCAACATAGCAAGATTCCATCTTTACACAAAATTTAAAAATTGGCCAGGCATGGTTGTACATTCCTGTAATCCCAGCTACTCAGGAGGCTGAGGTGGGAGGATTGCTTGAGCCTGGGAGTTGGAGACTACAGTGAGCTGTGGCCACACCACTGCACTCCAGCTTGAGCAATGGAGCAAGACTCTGTCTCAAAAAAAAAAAAAAAAGGCCAGGCGCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCGCCTGAGGTCAGGAGTTTGAGACCAGCCTGGCAAACACGGTGAAACCCCATCTCTACTAAAAATACAAAATTAGCCCAGCGTAGTGGCGCATGCCTGTAATCCCAGCTACTAGGGAAGCTGAGGCAGGAGAATCGCGTGAACCTGGGAGGCAAATGTTCCAGTGAGCCGAGATCGTGCCATTGCACTCCAGCCTGGGCAGAGCCTGCTGGGTTGGGCTGGGTAAGCTCTGAACACCAGTCTCATGGCTTCAAGTCACACCTCCTAAGTGAAGCTCTGAACTTTCTCCAAGGACTATCAGGGCTTGCCCCGGGCAGAGGATGCCGACACTCACTGCTCTTACTGGGTTTTATTGCAG
The "ACAGACTACCACATGAACATGATGCACATCTACGTGTTCGCAGCCTATTTTGGGCTGTCTGT GGCCTGGTGCCTGCCAAAGCCTCTACCCGAGGGAACGGAGGATAAAGATCAGACAGCAACGA TACCCAGTTTGTCTGCCATGCTGG" sequence segment is: rhdexon4GTAAGGACAAGGTGGGGTGAGTGGTCTCCTACTTGGGCTGAGCAGAATGGCTCAGAAAAGGCTCTGGCTGAAAAAATCTCCCTCCTTTACCAAGTTCCCCTGGGTGTCTGAAGCCCTTCCATCATGATTCATTTCTTTGAGTAGTGTTTGCTAAATTCATACCTTTGAATTAAGCACTTCACAGAGCAGGTTCAGGAGGCCTGGGGTATGCAGATTTCAACCCTCTTGGCCTTTGTTTCCTTGTCTGTAAAATGTGGTTAGCTGGTATCAGCTTGAGAGCTCGGAGGGGAGACGTGACTTCCCCATCTAACTCTAAGTGACAAGGCTGAGACTCTCCAGCCCTAGGATTCTCATCCAAAACCCCTCGAGGCTCAGACCTTTGGAGCAGGAGTGTGATTCTGGCCAACCACCCTCTCTGGCCCCCAG
The "GCGCCCTCTTCTTGTGGATGTTCTGGCCAAGTTTCAACTCTGCTCTGCTGAGAAGTCCAATC GAAAGGAAGAATGCCGTGTTCAACACCTACTATGCTGTAGCAGTCAGCGTGGTGACAGCCATC TCAGGGTCATCCTTGGCTCACCCCCAAGGGAAGATCAGCAAG" sequence segment is: rhdexon5GTGAGCAGGGCGCTGCCCTTGGGCAGCACTTGGGTCTAACAGGACTAGCACACATATTTATGCCCCTCCCCACCCCAGGGCCAGCGTGGGTTGGGAGAGGGCATGCCGGGTGGTGGAGCTGTGCCTGCCTCTACAGTGGAGCTCTAGGTAGAATGCTGGGTGGTCACAGTGGGCCTGGGACTCAGGAGACTGTCCAGTGATCAAAGGCTTTCTGGGGGGAGTGATTAAATCCATCCATGCTAACATGAAACAGACCTCAGTTTGAACCCCATTTCTGCTAGTTGCTAAAGTCAGTCACCATGAGCGAGAGTCAGCAGCAACAGACTAGACTAGAATTAGCCAGCCTCTCTCTTCCCCCCAACAAATTTCAAGAATGGAACCATCAGAATCAGAAGTAGAGAAGTATGTGACACTAGCCATGTGGCTCTGGTCAAGCCACTTCAACGTTTTGAGTCTCAGTGGCCTCATCTGTAAAGTGGGAATTAAGAGATGGTGCATGTAAAGTGCTTAACGGGGAGTAAATGGTAGGCAAACATTAGCTGCTGCTATTAGTAAAGAGAGACGATGGTGTGTGTGAGTCTTGTGGGCAGAGATGGGTGAGAGGGGAGACAAAACAAGTTCTCATGATGATGGGGGAAGGGGCTCCAGCTGGTGGTGTCGGAGGGAAGTCTGGACAGACCAGTGGTGGGGCTCGGGTGGGAGGCACTGGGGGGGCTGGAGTGGAAAGAATGTGGCCACAGATGACAGCTTCACAGCAGAATTCAGTGCTAAGAGGAAGTGAGTGGCCATGAGTTCCATGGTGACAGAAAGTCTAAGACACCCAGCAAGGCAGGAGTGGGTGTCAACTCAGGGAAGCCCAGAGGCTAATCCTAGGTGAGAGCTGAGGGTGTCAGATAAGAGCAAGGCAAGGCTCCGGTTCTGGAGCAGTGAAGGACATAGCAGAGCTATGACCCAGGAACAAGGCCCAGCTTATTGAAACTGGGCCCAGTCACACAGGGTGGCACAGGCACCAAGTAGCCAATAATAATAATAAAAACAATAACAATGATTTGTGTCTACTGGGCATTTATTCATGTTCTATGCCAGACACTGGGCTAAGAGCTTTATATGTGGAAACTCATTTAATCCTTACAATAACCTTATGAAGAAGGTACATCCAAAACCCCATTCTTCTAGGCCAGGTGCAGTGGCTCACACCTGTAATCCCAATATTTTGGGAGGCTGAGGCAAGAGGATTGGTTGAGGCCAGGAGTTCAAGACCAGCCCAGGCAACATAGCAAGACCCTGTCTCTAAAAAATAAAACAAAAACCCATTCTTCCCGCTGCCCAGGGACACACCACTAATGAGTGTGATGGGTGCCTAGGATGCTGAGCACCTGGACTTCCCAGCTCATTCCCTAAATGCTGCACAATCAGGGTAACTGTGCCCTGAGCCTAAGAGGCAGTAGTGAGCTGGCCCATCATGTCCACTGATGAAGGACACGTAGCCCCAACACAGGGGAGAAGTGGTTTCAGGATCAGCAAAGCAGGGAGGATGTTACAGGGTTGCCTTGTTCCCAGCGTGCTGGTCACTTGCAGCAAGATGGTGTTCTCTCTCTACCTTGCTTCCTTTACCCACACGCTATTTCTTTGCAG
The "ACTTATGTGCACAGTGCGGTGTTGGCAGGAGGCGTGGCTGTGGGTACCTCGTGTCACCTGA TCCCTTCTCCGTGGCTTGCCATGGTGCTGGGTCTTGTGGCTGGGCTGATCTCCGTCGGGGGAG CCAAGTACCTGCCG" sequence segment is: rhdexon6
GTAAGAAACTAGACAACTAACCTCCTCTGCTTTGGCTGAAGGCCAGCAGGACGCTGGGACCT
GATGGGCCACTGTGCAGTGCACAGCTGCATTAGGCAGGTGTCGGCGCATTCTCTTATTGGCTT
CAACGCCTAGTGAGGGATCCATCCTGGCTCGGTGGCGCATTTGTTAAGATGCTCGGGAGCAGG
TGGCAGAACCCATTTGAGCTTGCTTGGGCATTGGGGAGAATTTGTTATCAGGCTACTGGGGTG
TCACAGAACTCAAGGACAGGGACTGGAGTGTTGTGGGGAGCCCCGAAGCCCCTGTTTTACTT
CTTTCTTTGCTTTTCCTGAATATCTGCTTTATTCTTACTCTATAGACATGCTTCCTCCTCTTTCAC
CCCACATTGTGGGGTGTAGTCTTTTGCTTCAAGAAAGCAGCCTGGTGGATGGAATCTCTTGGC
CCCAATCCCAAATTCTCTGGAGAAGGGGCTCTTTGGTTTAACTTGGATAATGTTGTCTTCAGCT
GGGGGTGGGCACATCGTGCATATGTGGCTGCTGCCGGGGAACCACGTGGATGATGTGAGAGG
AGCAGCACCCAGAAGAGGGAGTGCTGGGCTGATGGTCCAGGTCGTGTCCACTTCTGATTGTTT
AATTCTTCTTCTAAGTGGATGGATCTTTCTCCAATACTCAGCAAATCCTGATCGTTCCAGAATAC
TTCATTATAGCCAATTGGTTATAATGTGCTTCTCTAAGAGAAATATTTAGGGACAACAAATCTTC
ATGGGTTTGAAGACTTGATGGAGGAAAAAGGAGTAGATTTTCGAAGGCTGGATTTGGATGAA
CAGGGGCTATTCAGGGAGTGCATTCCAACCTAAAATTAGGAAAAACTGGCTGGGCGCAGTGG
CTCACGCGCTTTGGGAGGCCGAGGCGGGCAGATGGCCTGAGGTCAGGAGTTCAAGACCAGC
CTGGCCAACATGGTGAAACCCATCTCTACTAAAAGTACAAAAATTAGCCAGGCATGGTGGCGG
GCACCTGTCATCTTAGCGACTCAGGAGGCTGAGACACGAGAATCACTTGAACCTGGGAGACA
GAGCTTGCAGTGAGCTGAAATCGTGCCATGGCACTCCAGCCTGGGCGACAGAACAAGACTCT
GTCTTAAAAAAAAAAAAAGTGGTTTATATACAGAGTGGAATATTATTTAGCCATAAAAAGAATG
AAATCCTGTCATTTGCAGCAACATGGATGGAACTGGAGGTAATTAAAAAATAAAATTAAATAA
GGAAAAACGTATCAATACTTCGATTAACCAAAACCAGGGCAAATCTGATTTTCATCTTTGCAA
GGGGAACAAATTTCTTTTATCTCCTCTGGCTTTGAAACCCTGAAATGAAAGGAGGAAGGGCA
GAAAAAAGAACACATAGCAAGTTATCATCAGTCTCAGCGCCCATCGCATTCCCTGAGCTTGTT
TCCTTGACTTCATCACTGGCAGGACTATTCAAAAATGTTTCGCTCATTCATTCATATATTCATTCA
TTCATCATTCCTTCATTCAACACATACGTTTTAACACTCATCTTGCTTTTCAAGCTATAGTTTAGT
GAGCGAAATGGATACACACAATACAGTGTGAGAACAGCAAGAGGGCACATCTGAGCTAGCCT
GGGATGGGTCTGGAAATGCTTCCTGGAGCAGAGGAAACGGTTGACAGCCAAGTGTTGACAGA
TAAGTAGTATTAGCCAGGCAGAGACATGGGGAATGTATTCCAGGCAGAAGGCACAGTGTGTAT
GAAAGCTTATTGTTAAGAAGAGTGTGTGGCCCAACCAGGAAACAGACATTCTAAAGGCATAG
GGTCCACCCAGGAGCATGGTGGACCCAGATCCCTGAAAGATGGGAGGTGCTCAGGCACACTT
CCTGGGCTAGTTGAGGAGTCTGGATATTTATTTATTTATTTATTTATTTATTTATTTATTGAGACAG
AGTCTCATTCTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCTCAGCTCACTGCAACCTCCAC
CTCCCGGATTGGGTTCAAGTGATTCTCCTACCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGC
CCACCACCATGCCTGGCTAATTTTCGTGTGTGTATGTATTTTGTTGTTGTTGTTGTTGTTGTTGT
TGTTGTTGTTGAGACGGTGTCTCGCTCTTTTGCCCAGGCTGGAGTGCAGTGGCGCCATCTCAG
CTTACTGCAAGCTCCGCCTCCCGGGTTCACACCATTCTCCTGCCTCAGCCTCCTGAGTAGCTG
GGTCTACAGGCGCCCACCACCACGCCCAGCTAATTTTTTGTGTTTTTAGTAGAGACGGGGTTT
CACCATGTTGGCCCTGCTGGTCTTGAACTCCCGACTTCAGGTGATCCACCCATGTCGGCCTCC
CAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAACCTGGATTTTTATTCTGAAGACTA
ATAGGGATTCTAAGGAAGGAACCAGCCTGATTGAATTTGCATATGTGTCCACATCTGCTGGCTC
ACGGCTGTGTGGGAGGCTGAGTGATGGGGAGGAAGGATTACTGAGTAGGGATCTGAAGGTGT
GGCCTCATGCTTTCTTTCTAACCAGCTGTGTTGTCTTTGGGATGGTGCTTAAATTTGGGCTAGA
CCAGTGGGTCTTGGTCACCCCCCAGGGGACATCTTACAATGTCTGGAGGCGTTCTTGGTTGAC
ACAGTGGGGTGAGGGCTGCTACTGGCAGCTCGTGGGGAGAGACCAGGGATGCTGCTTAACAT
CCTACAGTACACAGGGCAGCCCCCACCACAAGGAATTATCAGCTGAAATTGTGAACAGTGTCT
ACACTAGACCCTTGCTACTCATAGTGTGGTCCGTAGACCAGCAGCATTGGCATCACCTGGGAC
CTTGTTAGAAATGCTGTTAGACCCCACCCCACATCCACTAAAGCCAGCTCTTCATTTCAACAA
ACTCCCCGATGATGTGAGTGCACATTCAAGTCTGAGAAGGGCTTCTTTGAGGTGAGCCTTAGT
GCCCATCCCCCTTTGGTGGCCCCGGATACCAAGGGTGTGTGAAAGGGGTGGGTAGGGAATATG
GGTCTCACCTGCCAATCTGCTTATAATAACACTTGTCCACAG
The "GGGTGTTGTAACCGAGTGCTGGGGATTCCCCACAGCTCCATCATGGGCTACAACTTCAGCTT GCTGGGTCTGCTTGGAGAGATCATCTACATTGTGCTGCTGGTGCTTGATACCGTCGGAGCCGG CAATGGCAT" sequence segment is: rhdexon7
GTGGGTCACTGGGCTTACCCCCCATCCCCTTAACACTCCCCTCCAACTCAGGAAGAAATGTGT
GCAGAGTCCTTAGCTGGGGCGTGTGCACTCGGGGCCAGGTGCTCAGTAGGCTTCGGTGAATAT
TTGTTGGCTGATTTATTCAGAAATTCTGTCCAGCCCCTACCTTGGATGGATTTATCACCTCTCCA
GGCCACCTCTTCTTTCCAAATAGGGCCACCTAGGTATAGACCAAAGACACGAAATCTTTTGTG
ATCCCACAAACACAGAGCAGGTCAAATAGGCCCAAGCCAATTGAGACTGTGGTTCACGTCGT
GATGCAGAGCTTTGCTGTGGACGTGCTCCCACTGCGTACTAGCTGGGCATGTGGCTTAACCTT
TCTCAGCCTCAGTCGCCCCATTGTAAATGGAGATAATGATACTATCTCCCCTCACAGGACTGTT
GGGATGCTACTGGATTTAATAAGCTAATGCAGGGACATGCTAAGCACAACCCATCCCTGAGGC
CCAGAGAGGGGTGGGCCTTGGCTGAGGTCTCACTGCGAGGTGGGAATGTGGGCCTCCAGACC
AGAGGTAGGTCCTGTGGCCCCTAGACAGTGGACAGCAATGGTCAGTTTGACACACCAGAGCC
CTAGCCATTACTTCCTGGATGTTGTGTGAATATTTTCTGGACATGGCTTATATAAAATGAAAAAG
TGAATTGGGCACGATACAGGGATAGATTTTTAGAGATGAACTGGTAGCATGATGATAATCATAT
TCACTGATAACATTTACTACTGTTATTGACTGCTTTAAAAGTGTTGGGCATTGTGCTAGAAACC
ATTATATGCATTATCTCCTTGAATTCTCACAACCGCCTACTGAGGTATTCTCAGACTCTAAGAAA
TGAGATTTAAGAGAAGTTATCTGCCCAAGGTCACTCGGCTGGAACCTGGCTGTAAAAATGGCT
GAAGCAGGTGATGAGGAGCTGATGCGTTTGGACGTGTCTCAGAGAAATCATGGAGGCGCTGC
GGTTCCTACCGGTTCTTGGATGCCTTCTACAGAGACAACCATAGCCCCAAATTATAGGGATCAC
ATATCAGTGGGTGAGACATCCTTGCTTGGGATGAGGAGGGGATGAGCTGTGTGAAGCAAGGC
GCCTCTGTGATGGGTTCCAGTGATGTGTCTGCCACTGTCTTAATAACTGTGCAATTCTAAGCAG
AACCTTTCCTGTCTCTGGGCCTGAGAGTTCCCCTCTGAAAGATGAGGACTTGACCTAGCAAGG
TCCTACTCACATGCCTGTAGAGAACAGGCAGGGGAAGTTAGAAAAAAAAAAGCCAGTGAAG
GAAGGGAGCTCTTCAGCTTGCACCCATCATCACAGTGCAGGGACCCAGGCTCAGTGTTGCCA
GATCCAATGACTTCTCAAGAGCTCAAAATCTAGAGTTTTGCATGTGCTCTCCCAAGTACTGGC
AGAAAATTCAAGATTGTTAGTAACACTGTGTGGCTAAATTCTGCTTGTGGGCTGCCTAGATTCC
CAATTCTGTGATTCTGTGGTTCTCTGGAAGCATTGGTTCTCCACAGCACCTGCATCACCTGGAA
ACTTGCTAGAAATATAAGCCCTACCTACGGCCCCACCCCAGACCTACCCAGTTAGAAATCTGG
GGGTGGGACCTATCAGTCCATGTTTGAATAAGCCCCACAAGTGTTCTCTTGCAAGCTCAAGTT
TTAGAACCACTGACCTATAGCCAAAAAAGAAAAAGCCAATCAGTGGTTTTCTGGTAAAGGATT
AACTTAACAAACTGGCTTTCCAAGAAAATAAAGCCTTGATTGGTAGCACTTGCAATTTCTATG
GTACAAACGCTTCCCGCATGACTGAGTTCAAGCTGTCAAGGAGACATCACTATACATGGACTT
GGGAAGAGATGAGAACAATCAGCCCACTGAGCCTATGGGAACTGGCTCCAGCACATCCCTGC
AAGTCAACTCTCATCAGGGTGAGTGAGTTGAGGACCAAGAAGCAGTTATCCTCTTGCCTTTGC
AGGACCCAGGCAAAGGGAAGGGCATAGTGACAGTGATGATCTCTCTTCCGGAAGTCTTTGGT
TTGCTGAGAGTAAAAGGCGTGGGCTTCACCAGTGGTGAAGCCAGTCATGCAGCCTTAGTCCT
GGTACTGAAACTCTCTAAATCTCAGTTTTCTATCTGTAAAATGGGAAAATAAGACCTATGTCAC
AGGGTTGCTGTGCAGATTTAGCAACAGAACATAGCCCCGTTCTTTATGATGACTGATGCTGCAT
CCGTATGAGGACATCTCTATGTAATGGAAAGATGGAGAGAGGATTAAGCGCAAAGTCACAACA
CTTAATGGGAACTGTGGATTAGCTACTTGGTGGCATTGGGCAAGTCAGTTGACTTTGCATTAAT
TCCACAAACAATATTTCCCAATTTCCTATTCAGATGAGCATATGTGATTGAGTCAGATGCTGTGA
TCAGAACCAGGATGGAGCATTTCCCACAAACTGTGGGATTTTTAAGTAATGGGAAGGCACACT
GAAATGGCACTGAATCATGCAGTTGCAGATACTCTTTTTCAATTCTCAGTCCTTTGATTACGTC
AGGGAGAAAAGAAAGTCCCCACTTGGCCTGAGAATCTCTGCACCCTTCTAGCTCTTGTTAACC
ACTCTTTTGAATAGCAGAGAAAACCTCAGACTGCCATATCTGGGAGAGATTTTAGCAACATTTT
GTTTTCATTGTATCTCTTTTTACAGCTACCTCCCATTTCCCTTCTATTTCAAGCTAGTAACTCAGT
TTTCTTTTAAATTCAATTATTTAAATGTAAAAATAAGTCTATTTGGAGAAAAAAAATTTTAATAG
CATCTCTGGAATGCCAGTATGGCTAAATTCATGAATGTTGTCCTCAAATGCTGAAATCTGGGAA
GCATCTGGCCAAGCTTTGTGGACAGGCCTGCCTAGTTTGAATCCCAAGAGCCACCCAGTCCAA
GCCACAAAACATTGGAATTCTTGGTTCACTTCCCTAACCTGAACTTGCCCTCTGTGAAATAGG
GACACTAATAGCTCACTCACAGGGCTGCTGTGAGGACATGTGTTGAGCTGAGGGTCTCGCCA
GGGGAGACCCTGTGCAGGGAGACTGTTATCATGGTGATGGATTTCTGCTTCATTCATTTCTTTT
TCCAGACAGCATCATATAGAATGAGTTGTGGGGTGGCAGTCAGCAGGTTTGGGTTTATCCTCTA
TTCTGCCACTTATTACTTAAAAAAACCCCAAAAAACCCAACTTATATAGTATAAGCTATATCCAG
AAAAGTGCAAATATCATACAAGTACCATTTGATGAATCTTCTGATATCCCCACATAACCAACAC
CCAGAACCTCTTCTTGTCTCATTCCAGGATAACCACTAACCTGACTTCTAACAGCATCAGTCAG
TTTTGTCTGTTTTTGTACATTATATATGTGATGGTTTGAATGTGTCCCCCAAATTTCATGTGCTGG
AAACTTAATCCTTCAATTCATATGTTGATGGTTTTTGGAGGAAGGGCCTTTGGGAAGTAATTAG
GATTAGATAAGGTCATGGGGTGAGGTATGATGGCACTGGTGACTTATAAGAAGAGAAAGAGAA
ATCTGAGCTGGCATGCTCTTGCCCTCTCACTGTGTGATGACTTCTCCATGTCATGATGCAGCAA
GAAGGCCCTCACCAGATGGTGGCACCATGCTTTTGGACTTCCCAGCCTCTAGAACTGTGAGCT
AAATCAATTTATTTTCTTTATAATCACCCAGTTTGATATTTTGTCATAGCAACAGAATATGGACA
AAGAAAGAAAATTAATGCAAGAAGTAGAGTTTTTACTGTAACAGATTCCTGAAAATGTGGAA
GTGGCTTTGGAACTGGGTGATGGGAATAGGTTGGAAGAGTTTTGAGGAGCAGGCTAGAAAAA
GCCTGTATTGTCAAGAATGGAGCATTATGCCAGGCACGGTGTCTCAGGCTTATAATCCCAGCAC
TTTGGGAGGCCAAAGCAGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTAGCTAACA
TGGTGAAACGCTGTTTCTACCAAAAATACAAAAAATTAGCTGGGCGTGGTGGCGCACACCTGT
AATCTCAGCTACTCAGGAGGCTGAAGCAGGAGAATCACTTGAACCCAGGAGGCAGAGGTTGC
AGTGAGCTGAGATCGTGCTATTGCACTCCAGCTTGGGCAACAAGAGCAAAACTCCATCTCAA
AAAAAAAAAAAAAGAAAGAAAAAGAATGGAGCATTAAAGACAGTTCTGCAGTTCTGGTGAG
GGCTTAAAGGAAGACCCCAGAACTAGGGAAAGTCTGGAACTTCTTAATGGTTACTGAAGTCG
TTGAGATCAGAGTGCTGATAGAAATATGGCTGGTAAAGGCCATTCTGATGAGGTCTCAGATAG
AACTGAAGAACCACGTGTTGGAAACTGGAGCAAAGGTCATCCTTTTTATAAAGAAGCAAAGA
TCTTAGCTGAACTTTTTCTGTGCCAGAGTCATTTATGGAAGGCAGAAAATCTGTAGGTCAGCCA
TGTTGTAGGGAATGAAAGAACATTTTCAGCTGAGAACACTGAGAGTGTGACACAACTACCGA
CTGATAAGAAAACTAGTACACATAAATTAGCCAGGCGTGGTGGTGGGCGCCTGTATTCCCAGC
TACCTGGGAGGCTGAGGCAGGAGAATGGCATGAACCCGGGAGGCAGAGCTTGCAGTGAGCC
AAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCAAAACTCCATCTCAAAAAGAAAA
AAAAAAGGAAGAAAGAAAATTAGTACACATAGAACAAAGCCAGAGGCTGTTCATCAGGACA
AGGGAGAAAAACTCCAAAGCCATTTCAGAGATCTTCAAGACTGCCCCTCCCATTACTGGCCCA
GAGCTCTAAGAGGGCAGAATGGTTTGGAATGACCAGCTGCTGCCCAGGGCTGCCTTGGGTCT
CGTCTCCCCACATTTCTGGTGCAGCATTCCTCAGCCATCCCAGCTGTGGTTCAGGTGGCCACA
GGTGTGATGTGGAAGGTAAAAGTCATAAACCTTGGCAGCATACACATGGCACTAATTTTGCAG
GTGTGCAGAATGCAAAAGCTGAGGGGGCATGCCTTCTTCCACCTACATTTCAAAGGGTGCTGT
GAACAGCCACCCCAGAGAGCCCCTAGTAGAGCAGGGTCTAGTGGAGCTACAAGGGTGGGGC
CACCGCCAAGACCCCAGAATGGTAGAGCTATCATAGTGCAATGCCAGCTTGGGAGAACTGCA
GGCATGAGACTCCAACCTGTGCGAAGTGCAACATGGGCAGAACCCAGCAAAACCACAGGGG
CAGAGCTCCCCGAAGCTTCGGGGGTCCAAATTCCATAGTGTGTCCAGGAGGTGGCACACAGA
GTAAAAGATCATTCTGAAGGTTTAAGGTTTAATGTTGTTTTCTATGTTGGGTTTTGTACTTTCCT
GGAACCAGTTACCCTTTTTCCCTTGCCTCTTTTTCCTTTTAGAATGGGAATGTCTGTCCTATGCC
TGTTCCACTGTTGTATTTTGGAAGTCAATAACTTGTTTTGACTTTACAGGCTTACAGCCAGAGG
GAATCTCCCATAGAATGAATTGTACCTTAAGTCTCACCCACATCTGATTTAGATGAGACCATGG
ACTTTGGAATTTTGAGTTGGTGCTGGAACAAGTTAAGACTTTGGGGGTTGTCTAAGTGTGGTG
TTTCATGCCTGTAATCCCAGTGATTTGGGAGGTTGAGGTGGGAGGATTGCTTGAGCCCAGGAG
CTCAAGACCAGCCTGGGCAACATAGTGAGACCTGTCTCTACAAAAAATAAAAATAAAAAAATT
AGCCAGGTATTGTGGCATATACCTGTAATTCTAGCTACTCAGGAGGCTGAGGTGAGAGGATCA
CTTGAGCCCAGGAGTTTGAGGCTGCAGTGAGCTATGGTCGTGCCACTGCATTCCAGCCAGGG
CAACAGAGTGAGACTCTGTCTCTACAAATAAAATTAAATAAACTTAGCTGGATATGGTGGCAC
ACATCTGTAGTCCTAGCTACTCAGGAGGCTGAGACAGGAGGATTACTTGAGCCAAGGAGTTTG
AGGCTGCAGTGAGCTATGATCATGCCACTGCATTCCAGCCTGGATGATAGAGCAAAATCCCAT
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTTAGTGCTATTGGAATGAATT
TTGCATGTAAGAAGGACATGCATTTTGGGGGCTGGGGCAGGATGCTGTGGTTTGAATGCATCC
CTCAAATTTCATGTGTTGGAAACTTAATCTCCAAATTCATATGTTGATGAAATTGGAGGTGAAG
CCTTTGGGAGGTAACTAGGATTAGATAAAGTCATCAGGGTGGGGCCCCTATGATGAGACTGGT
GGCTTACAAGAGGAAGAGAGAACTGAGCTGACATGCTCTTGCCCTCTTGCCATGTGATACCCT
CTGCCATGTAATGGCAGGCACAGCAAGAAGGTCCTCAACAGATGCCAGCAGCATGTTCTTGG
ACTTCCCAGCCTCCAGAACCATGAGCTATATATACTTATTTTACAAATTACCCATTCTGTGGTATT
CTGTTATAGCAATAGAAAATGAACTGAGATAATATACATGGAATCATACAGTAAGTCTGTGCTTT
TGTATGCTTCTTTTACTCAACATTGTAGTTGTGAGATTCATCCAGGTTGTTAAGCATTGCTGTAC
CCTTTTTCCACTGGGATATAGTGTTCTGTCATGCTTGGGTCTTAATTTATAAAGGTGACTGAGTG
GCATTTTCTTCCAGTATTATTGGAAGGAAAGTTTTGTTGTTCACAGTTCCCCTGTAAACAAGAG
GCAGAACACGTCATGCAGGGCCACACAAAACTGTATCATCCAGGGACCAGGCAGCAGAAAG
AGAGGGGGAACTGGGACTATGCGTTTATGAAAAAGAGTGGTGGGAGAGTAACTGGGTGAGG
GCATCCACTAATGGGCAGGAAGTGAAAACACATATGTTAGAATTTGTAGCTGAGGGGTTTATAA
TATGAGTTTCCTATGCCTGAGAAAGCTGACTTGCAAGAAAATGAGATAAACAACTTTGGCCAT
TAGTGTGGCCCTGTCATAAATGAATGCCAGATAGGCAAATAGAGAATCTAAGAAAAGATAGTT
GGAACAAGTGTTCCATTGTGTGAATGCAGCAGAATTTATTTATCCATTATTGAGGAGGATTTGG
GTAGTTTCCAGTTTGGAGCTATTATGAATATTCTAGTATTGCTCCTATGAACATTCTAGCACTTTT
ATTTTTGGAGCACACGAATGCACTTCTGTTGATTATATGCCTAGAAGTGAAATTGTTGAATTATA
CAGTATTCACACAGTCAGCTTTAGTGGCTACTGCTAAACAATTTTCTCTAGTAGTTTGCGCCAA
TCTAATCACCAGTAGTGTATAGAAGCTCCTTTTACTCCACATTTTGCCAACACTTGGTGTTTTCC
TTCTTTTTGATTAGTCATTTAGCAATCAAACCTATTGTTTACATTTTGATATCTCCAATAACTAAC
TAAATGGAGCACTTTTAATATGCTTTTTGGACAGTTGAATATCTTTTCTTGTGAAATGTCTATTC
AAGTTAGTTTGCCCATTTTCTATTGTGGTGTTCTGTCTTTTTCTTATTGATTTGTAGGAATTCCTT
ACGTATCCTGGATATGAATCCCACTTTGTGCGTTACCTTTTTCCTTCTTTCTTTCTTTTTGAAAC
AGAGTCTCCTTCTGTCACCCAGGCTGGAATGCAGTGGCGCTATCTCAGCCCACTACAACCTCT
GCCTCCCAGCTTCAAGCAATTCTCATACTTCATCCTCCTGAGTAGCTTAGATTACAGGCGCATG
CCACCATGCCCAGCTAACTTCTGTATAGACAAAATAATTTTTGGTAGAGACAGGGTTTTGCCAT
GTTGGACAGGCTGATCTTGGACTCCTGGCCTCAACTTTGGCCCACCTTGGCCTCCCAAAGTGC
CAGGATTACAGGTGTGAGCCACCATGCCCAGCCCACCTTTTACTTTCTTAATGGTGTCTTTTGA
ACAAGAGAGGTTCTTAATTTTAATATAGCCCAATTTATCATTGTTCCCTTTATGTTTAGTTCTTTT
ATGTCCTTTTTAAGAATTTTTGCAGCCAGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGG
GAGGCTGAGGCTGGCGGATCACAAGGTCAAGAGATCGAGATCATCCTGGCCAACATGGTGAA
GCCCTGTGCCTACTAAAAATACAAAAAATTAGCTGGGCGTTGTGGCTCTTGCCTGTAGTCTCA
GCTACTCGGGAGGCTGAGATCACGCCACTGCACTCCAGCCTGGTGACACAGCAAGACTCCAT
CTCAAAAAAAAATTTTTTTTGCAAGGTCATGCATATGTCCCCCTGATTTTTTTCCTAAAAATCA
CTTATTATTAGATCAATGAATTGAGTAATTGACTACATTTTTCAGTCATTCAACAAATATTTCCCT
GAGGTTTTGATAACCTGAACTGTGTTTGGAGCTGGGGAGGAAGCAAACTATTGAAGATATACA
AAGATGGCAAAGATGAGGGCCTGGAGCTTGCCACACGGAAGGGGGGATGGCTGCCTGAATG
GTTGGGCAGGTAGTTGTTGACATCTGCACTCCCTACATGAGCAGCAGGGTGGCAACTCTTTTT
ATCTTTTTAATTTATTTTTCTTTTCTTTCTTTCTTTTTTTTTTTTTGAGATGGAGTCTCGCTGTGTT
GCCCAGGCTGGAGTGCAGTGGCGTGATCTCAGCTCACTGCAAACTCCACCTCCCAGGTTCAC
GCCGTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCTGCCACCACTCCCGGC
TAATGTTTTGTATTTTTAGTAGAGAAGGGGTTTCACTGTGTTAGCCAGGATGGTCTCCATCTCCT
GACCTCATGATCTGCCCGCCTCGGCCTCCCAAAGTGTGGGGATTACAGGTGTGAGCCACCACA
CCCGGCCTTAATTTATTTTTCTAGTCTGCAGGTAATTCTTTTTAATTCTCTCCACTCTCCTATGAT
CTTATGAGGTAGGGACTGTCATTATTTCTCCCACTTTATAATGAACAATCAGTAAAGACAGGGA
AGATAACCAAATGACATACAAGGTGGGGTCCACCCCATGAGGCTGCAGGCTTGGAGCTTTGCT
TTGTCTTAAAAATGAGAACATGAGCTGCCCACCTGTTGAGACAAGAAACAGGAAAGGCTTAA
AAAACTGGCTTGTTGTGTACAACTATCCGTGGGGCTGCAGTGAACGGGCTGGCAGTGCCCAG
GTGCAGGCTGAACCCTGGGACAATCACATTCAGCATCCAAGGGCCCCCGTAATAGCTTAATGT
TTGAATTGAACCCCTGGGGTTGCCTTGAAGGAGAGAGGTCGTGGAAGTATGTTCAAGGGGTA
GGGATGGGCAGGGGAGATGGGTCTGAAAGCCAAGCTCTACCCCACCCACCTTGCCCCAAGAG
AAATAGAACCTTCATCTTTAATTGCCTAACGAGAAAACTGGGGCTGGCCAGATGTGGTGGCTC
ATGTCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCAGATCACTTGAGGTCAGGAGTTC
GAGATCACCCTGGTCAACATGGTGAAACCCCGTCTCTATTAATAATACAAAAATTATCCAGGTA
TGGTGGCGCATGCCTGTAGTCCCAGCTACTTGAGGCACAAGAATCGCTTGAACCTGGGGGAC
AGAGGTTGCAGTGAGCCGACCACTGCACTCCAGTCTGGACGACAGAGTGAGACTCCATCTCA
CAAACAAAAACAGAAAAAAAAAAAAAAAAAAGAGAGAGAGAGAAAACTGGAGGCTCTGA
GAGGTTGAGGGACTTGCCCAGGGTCTTGCAGCTAGTAAGTGACAGAGCTGGGACTTGAGCTT
GGGTTTTCTGACTCCTGGTCTGGTTCATTATCCATGAGGTGCTGGGAACTAAAATAAGCCACAA
TCTTGGAATCTCCGTCGCCTCCCTCCCTCCCACATGTCTGCGTGGCTTTTTGGGAAAATGCCAG
GGGAATGTACCAGCCAGGGAGAGGACCCTTGTTTTCCTCATGGCCCTTCCTGGCAATGGCACT
ACTGACACCGACAGTCCTTTTTGTCCCTGATGACCTCTGCTGCCTGATGCCCAAGTGACCACC
TCTGCTTTGTCATTTCTAG
The "GATTGGCTTCCAGGTCCTCCTCAGCATTGGGGAACTCAGCTTGGCCATCGTGATAGCTCTCA CGTCTGGTCTCCTGACAG" sequence segment is: rhdexon8
GTCAGTGTGAGGCCACCTTTCTTCCACCATTGCCAGGACACAGCACCCACGTCCAGAGCGCA
CCCTGCCGTGTGGCTGGATGTCTATGTGCCCCATCTCCTTCCCTGAGGATCACATAATTTCAGA
ATTGGAAAGGTTCTTAGAGGTCACCTGCTGCTAATGTGGACTGTGAGGCCAGGGCAGGGAAG
GGACATCCCTGAGGTTATAAGTAGGGTGAGTGGCAACGTTGCAGACTTTTGAACCCAGGGCT
GGTGATCACACTCAGTTTTGCACAGAAGCCCGAGAAAATCCTTACACCCAAAAGCCTACCTTT
TATTTCTGAGGACACCCATAATACTATTTTATTCAACAGATATTTATTCAATATCCACTATGAGCC
AGGCACTGGGGACACAGCAGTGAGCAAAACAAATTCCCTGACCCCATGGAATTGACCTTCTA
GTGGGGGAAGGTATTAGCAATAAATAGACAAATAAGTGTCTACTACGCCAGATGGGAAGAAGT
GGCTGTGAAGACAGAGCAAACTAGAGAAACATAGAGTCAATGTGGGATGGGGTGTTCTTTTA
GGGGGGTGGTCAGGGAAAGCTTATCTGAGTAGTTAGCTTTTAAGCAGAGACCCCAATGAAGA
GGAGGGAGATATGCGATGCATTTAGTTAGGGGAAGAACATTCCATGAAAATAGGATAGCAAGT
GCAAAGGCCCTGAGACAGCAGCATGCTTTGTGTGTTGAGGGAACAGTAAGGAGACCAGTGTG
GTTGGTGTGAATGGAGTGAGAAGGAGCAGCAGGGGTTGAGGGCAGAATGGTAGTGAGGAGC
AGGCCCTTATAAAAGATGGGAAGCCACTGGAGATCTTTCAACAAAGGGGAAAAGTATGTTTCT
GTTCTTGCAATAAAATAGAACAGCAAAAAATCTAGGGGAGTTGCTAATTAGCCAGTTTTACTTA
TATGCCAGGTGAAAATATGTGGCTAGGTGCAGTGGCTCATACCTGTAATTGCAGCAGTTTGGG
AGACCGAAGTGGGCAGATCATCTGAGATCAGGATTCAAGACCAGCATGGCCAACATGGTGAA
ACCCCATCTCTACTAAAAATTAAAAAATAAGCCAGGCGTGGTGTTGGATCCCAGCTACTTGGG
AGGCTGAGGCAGTAGAATTGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGACTCT
GTCTAAAAAAAAAGAAAAAAAGAAAATACACATTCAGGCCAGGTGCAGTGGCTCACGCCTGT
AATCCCAGCACTTTGGGAGGCTGAGACAGGTAGATCACTTGAGGTCAGGAGTTCGAGACCAG
CCTGACCAACATGGCAAAACCCTGTCTCTACCAGAAATACAAAAATTAGCCAGGCGTGGTGG
CGTGTGCCTGTAGTCCCAGCTACTGGGGAGGCTGAAGTAGGGGAATGGCTTGACCCCAGGAG
GTGGAGGTTATAGTGAGTCGAGGTTGCACCACTGCCCTCCAGCCTAGGTGACAGAGTGAGAC
TGTCTCAAAAAAAAAAGAAAGAAAATATACATTCCATCCAGAACTGTTCACCTTTATTCTACA
AGCAAACATCTTTTATTGGTTAGACACCCATATATGTGTCCCTAAGCAGGAGGTGAATGCCAAA
TAAGAGACAAATGGCGTAAGACACTATGAGTTGTGTGACGTTGGGCATGTCACTTTACTCCCT
CTGAGCCTTGGTTAGCTTCTCTGTAAAATGAAAGGATTATGGTAACTAAGCTGGCTTCCTTCCA
GCTTTAACAAACTGTATGGAGGTACTTTTTGGAGTTACCTGGGTAATTTTTGAGTGTGAGATTG
GCTAGAATTGCTTTAATATACCATGTCTGGCCTTAGCTTTTTGCAGAGTCTTTGTGAAGAAGCA
GAGGCGGAGTAGCGTTAATTCCGTAAGTTAACGTTCAGTTCGTGGCAGCTGGCAATCCAACCC
TGGGAAAGGCTGCCGGATTTAGCAAAAATGCAAGGTGTCTGTTTTTAAATTTGAAATGAATTG
GGTATCCTGCATTTTATTTGGCAACCCTGTCCTGGGACTCACACTATTCACTGTTATCACTGGTA
TGTTCAAAGTGGTGCTGACTTGCCCTCTGTCTTGCAAAGTACCAGGAGGTCTTTTCTTATTCTT
CACTGGAGTCAAAAAAGAGAATAGAGGAAAAGACAATCATATTGTTCCTTTAAGAGTTAAGA
CCAACAAGTTTTCCTCTTTACATGTTGTTTTTGACATGAGCAAACTGGTGATTAAAAACAACTT
GGGTGGCTCATACTTGTAATCCCAGCACCTTGGGAAGCTGAGGTGGGAGAATAGCTTGAGGCC
AGGAGTTCAAGCCAGGGCAACATAGTGAGACCCCATCTCTACAAAAGATACAAAAATTAGCC
AGGCGTGGTGGTACACCTGTAGTCCCAGCTGCTCTGGAGGCTGAGATGGGAGGATCAGTTGA
GCTTGGGAGGCAGAAGTTGCAGTGAGCTGAGATCATGCCACTGCACTCCAGCCTGGACAACA
GAGCAAGACCCTGTCTCAAAAAAGGAAACAAAACAACTTGGACAATGGAAGGGGGAAAAA
GTTCCTCAAGCAGCCAAAATTGCACCAAATGGACTCCCAGAAGACAAGCATTTAATTTGTTAA
TTGAGCCCTCTATGGGCCTGTCTGTATTTATTTAAGAAACAATCCTATCAAGCATAGTTATTGGG
TTTCTCAGCCCAGGTAGATTAGAAATAGCAGATTAGAGGTGGGCTAGGTTTCTAGAGGTAAAG
TACACCAGCAGAAGTTAGAAGTGAAAGCAAAGAGCCTAACAGAGGAAGAGAAATTCTTTTTT
TTTTCTTTTTTTAGACGCAGTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCTATCTCG
GCTCACTACAACCTCAGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCT
GGGATTACAGGCATGCACCACCACACCCGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTTCT
CCATGTTGGTCATGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCCACCTTGGCCTCCCA
AAGTGCTGGGATTACAGGGATAAGCCACTGCGACCGGCCGACAAATTCTTAAAACTGGACAC
AAGAACACAAAACGCTTGGGCTGCTGAGAGATTAGACCAACAACCCTCCACAGCTACACACC
TTTTCCACGTTATATGGCACGTTATAAGTGGGTGTTCCTAGTGATGGTTCTGATTTTTTTTAAAA
AAAGTCTAAATATGTTTAATGTTGTCTCAGAAGACAAAATATATTTTAGACAGATATTCCTCAGT
GATGAGTAAGCCTCAGCTATCTGGAAAATTCATGCAGGCGCCAGAGATCGTTACTGAGTAATT
CAAGCTAACTGCGTCATGCTGGTTGTACCCTGCATGCCAATATCAGCTAAAAGCAGCACCACG
AAAGGGAAATACGAATCTCACTAAGCACTCGCCCATTCTTGTTAACGACACTGGAACTGATCA
TCCTTAATAATACACAGATAAATCTATCAGGAGCATTTCCTTGCTTCCTGTGAAAGGAAGCACT
CATTCCATGTGTCCTGTGAAATTCATCCAACTTCAGGAAGCTGGAGGAATACATATGGCCAAG
CTATCTGGGCAGAGAGTAGACAGGGAATGGAGGTTGGGCACAGTGGCTCACACCTGTAATCG
CAGCCATTTAGAAGGCAAAGGCGGGCAGATCACTTGAGCTCAGGTGTTCAAGACCAGCCTGG
GCAACATGGCTAAGTCCTGTCTCTGCAAAAAATACCAAAAACTGAGCTGGATATGGTAGCACA
CACCTGTGGTCCCAGCTACTTGGGAGGCTGAGGTGGGAGGGTTGCTTGACCCCGGGAGTTTG
AGGCTGCAATGAGCTGTGATTGTGCCACTGCACTCCAGCCTGGATAACAGAATGAGACTCTGT
CCCAAAAATAAAAAATAAAATCAAAGACACTTAAAAAGATGGGGAAAAGGAAGGACAGGCA
CTTAAGCAAGTTATAAGCTACTTTCCTAACTACACAAGTGGAATCTTAAGCTGAGGTTCCCAG
GAGTTGACTGGAGCCAGAGAAGACAGACCTATAGGAGCACCCAATTGGAGTCACCCTCCATA
GTAGCCCATATGTCTTACATGGATCAGCTTTCGTGGGGCCCTTTTACTCCATCTGGGGAAGGGC
GTCAGATCTGTGGCTCTCATGTACTGCTCAGTACACTGCCATTCCCAGTTCTTTTTTTCAAAAA
AAAAAAAAAAATGTCTACAGAATCGGCCAGGTGTGGTGGCTCATGCCTGTAATACTAGCACTT
TGGAAGGCTGAGGTGGGTGGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGGCCAACATG
GTGAAACTCCATCTCTACTAAAAAAAAAAAAAAAAAAAAAATTAGCTGGATGTGGTGGCAGG
CGCCTATAATCTCAGCTACTTGGGAGGCTGAGGCAGGATAATCGCTTGAACCTGGGAGGCAGA
GGCTGCAGTGAGCCGAGATCACGCCATTGTACTCCAGCCTGGGCGATAGAGTGAGACTCTGTC
TCAAAATAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAGG
CTACAGAATTAAGCTGGTCCAGGAATGACAGGGCTTCCATTTATTTGTCTTTCAATTGTGGGAG
AAAAAGGATTTCTGTTGAGATACTGTCGTTTTGACACACAATATTTCGATTAATCTTGAGATTA
AAAATCCTGTGCTCCAAATCTTTTAACATTAAATTATGCATTTAAACAG
The "GTTTGCTCCTAAATCTTAAAATATGGAAAGCACCTCATGAGGCTAAATATTTTGATGACCAAG TTTTCTGGAAA" sequence segment is: rhdexon9
GTAAGATTTTTCACCTATTAACGTGATAGATTTTGAGTGCATGAACTTAAAAACATACCTGAGT
ATATATGTTGACTTGCTGTTTATGAGTAAAACAAAAACAAAAATGGAGTAAGGAGCATTGCAG
GAGGAACTAGAGGAGAAACAAATCCATGATATGCATGTGTGTGGGGGAGGGTGGCGGGGAGG
TGGTAAAGGTCACCATTTCCCTGATACCTCAAATTCATTCAGAGTCAGGGATGAGACAGCTTT
CACTGGCCACACTTCCCCTCCCCCTATCTGCAGTCCTCAGCGTAGCCAAATAGTCTGACATGC
GGGTGACAGAACCCCACAATGCAAAAGCTGGAAGAAACCTCAAGCCTTGGAGTCCAACCCC
TTTTTTGACAGATGCTAAGAGTGGAGACATGACTTATCAAGATCTTACAACTGGCTGGGCACG
GTGGCTCACGCCTGTGATCCCAGCACTTTGGGAGGCTGAGGTGGGGCGATCACCTGAGGCCA
GGAGTTCGAGACCAGCCTGGCCAACGTGTCGAAACCCCATCTCTACTAAAAATACAAAAGTT
AGCTGGGTGTGGTGGCACATGCCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATC
GCTTGAACCTGGGAAGCGAAGTTTGCAGTGATCTGAGATCATGCCACTGCACTCCAGCCTGG
GTGACAGAGCGAGACTTTGCCTCAAAAACAAAACAAAACAATTGTACATATTTAAAGTGTTGT
AACCAAGTGAGTTACAGAGAAACACCACACTTTGAGCCTAATTCAGGAGTCCTTTATTAGCCG
GCGACCTAGAGACGACTAGTGCTCAAAATTCTCTCGGCCCCAAAGAAGGGGCTAGATTTTCTT
TTATACCTTGGTTTAGAAAGGGGAGCGGGAATTGAGCTGAAGCAATCTTACAGAAGTAAAAC
AGGCAAAAAAGTTAAAAAGACAAATGGTTACAGGAAAACAAACAGTTCCAGGTGCAGGAGC
TTTAAAGCCATCACAAGGTGACAGGTGCGGGGGCTCTGGGTGCTATCTGCCGGACACAAACG
CAGGGGCACTAGAGTACTATCACCCGGGCAAATTCCTGGGAACTGCGGACACAGCTTGCCAC
AGTACCTTATCAGCTAATTGCACTCTTTGATGTGCTGGGAGTCAGCTTGCACAAGTTAAGTCCT
TGAGGAAGGGGGTGGGTAAGGAGCCCTTAACGTCTTGCAAATGAAGGAGCCGAATGGAATCC
CTCCGGCTTTCTTAGCTAAGAGAGAGTCAATCAAGTTAATACAAGTTAGGGTATCACAAAAGT
ATATAATTTGATACATTTTAACGTATTTATACACTGAAGAGACCATCACCACCATCAAGACAAG
GAGCACACCCATCACTTCCACACACTTCCTCCTGCTCCTTTGAAATTCCTCCCTTCCTACCCAC
CTGGTCCCACCCAAAGGCAACCACTGAACTACTTTCTGTCACTAAGGTTTGCATTTTCTGTAAT
TTTTTTGTTTGAGACAGGGTCTCACTCCGCCACCCACACCGTAATGCAGTGGCACCATCATGG
CTCACTGTAGCCTCAACCTCCCCAGGCTCAGGAGATCCTCCCCCCTCAGCCTCCTGAGTAGCT
AGGACCACAGGTGTAGGCCACCATGGCAGGCTAATTTTTGTATTTTTTTGTAGAGATGGGGTTT
CACCGTATTACCTAGGCTGGTCTCGAACTCATGGGTTCAAGCAATCCTCCTGCCTTGGCCTCTC
AAAGTGCTGGGATTATAGGCATGAGCCACTGTGCCCAGCCCTCTGTAATGTTACACAAAGGGA
ATCATGCAGCACGTACTGCCCTTGGTCTGGCTTCTTTTGCTCAGCATGATTATTCTGAGAATCAT
CCGTGTTGTTGCGTGTAACTGACTTCATCAGCTTCTCTCTGCAGCTGTCAGCTCTTGGCTTCTC
CCAACAGCCAATCTCTCTTTATCCCCTGCAAGTGTTCTTGCCTATTTAGCAGAATCAAGGTACT
CTATCGAAAAGACTCGGAAAATTGGTTTAATCTATTCATTCATTCCTCAGGTATTTATCGAATAA
CTATTCTATACCAAGTACTATGCTAATCAACCAAGGACAGCACAAACAGGAGAAATCTCCAGC
TCAGTCACTTGAGTTGCAATAAATATTTGCTGGATACGTCAGGTGCAGTGGCTCACACTTGTAA
TCCCAGCACTTTGGGGATTACTGAGACGGGAGGATCTCTTGAGCCCAGGAGGCCAAGGCTGC
AGAGAACCATGATCATGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGATCCTGTCTCTGA
AAAAAAATATTTGCTGGATAAATTAAGGAAATCTGACGAACCCCATCAGTAGCCATTGCAGCA
ACAGGTAAACTAGAACGAGTGTGAATTTGGAATGAGGAAACCCGATGTTGGCCATCATTCTGT
AATGTCATGTATTATGTAATGTATTATATATTAATGTATGTATTATGTAGGCAAGTTCCTTGACCTC
TCTCACTGGTAACATAAGAGTAGTAATCTTTGTGCTACTTCACTGGGTTATTTTAAAGATCAAG
TGAGGTAATAATGTCTGTAACAACATTCTGTAAAATGCAAACCGCCACATGAATGTGAAAGTTT
ATTACTAGGGATTTAGCCAACCACAAGGGAATGTGTGAGCATAAGAGCTATCATATTGCAAGCC
TACAGTTTCTGATTTTGTGCTAGGTGCTTTTCCACATTACCTGATTTTATCCTCACAACAGCCCT
GCATAAAAGTAAGTATGTCGCCCAGGTGCGGTGGCTCATGCCTATAATCCCAGCACTTTGGGA
GCCCGAGGTGGGCAAATCACTTGAGATCAGGAGTTTGAAACCAGCCTGGTCAACGTGGTGCA
ACCTGTCTCTACTAAAAATACAAAAAAAAATTAGACAGGCGTGGTGGTGGATGCCTGTAATCC
CAGCTACTTGGGAAGCTGAGGCAGGAGAATGGCTTGAGCCCGGGAGATGGAGATTGCAGTGA
GATGAGATTGCGCCACTGCACTCCAGCCTGGGTGACAGAGCAAGGCTATGTCTCAAAAGAGA
AAAAAAAAGTAAGTATCTCAGTCTTGAAGATGATGAAATGGAGGCCTAGAGAGATTAAGTAA
CTTGCCCAAAATGACAGAACTAATGCATAGAAAAGAAGAAATGTGATGTCTTTTGGCTCCAAA
GACACCCCACATATGCGTTGGTTACAGTTACTAGAGAAAAGTTATTCCACCCCCACCCCACCC
CCAGAAATCTTCTGACTTGTTTTCTCGCAGTTGAGTAGGACCATTTATTCGGCAGTGTACCATT
CTCAGCTTGCAGTTGAAAGCCAAATATCCATTAAAGAGGCAAGGATGCAAACTTGCTAAGCTG
ATAAATCCAGGGGTGATTTTTTTTTTTTTTGCAAACCATCCAACAAGACATTTTAAATACTCATT
GAATTTCATAGAACTGACTGCCAGGATTGGAAAGACATTAAAGCCAGCTCAGCCACTGCCTCG
CTGGTTGGCCAGACCACGCCTGGCACTTCTGGGAGGGAGCACTCACCACCCCCCAAGGGCAC
CCATCTCATCCTCCGAAGGTTTATGAAAATGCACTCATCATTTGCTAATTCATTCCACTACGTGT
ATTACCTAATTTGTGACACGATGTGAAGTACCAGAGAGATAATTCTAAATAAAATATAGTTATGG
GTCTCAAGGAGCCAGATATGCTAATCTCCTATCCTCCTGCAGTTTACAGTGGTCCTCACCAGAT
ACTTATTTACAAAAATTCAGTTTATTATTTATTTTTTTGAGACAGAGTCTTGCTCTATAGCTCAG
GCTAGAGTGTAATGGTGTGATCTCGGCTCACTTCAACCTCTGCCTCCCAGGTTCAAGTGATTCT
CCTGCCTCAACCTCCCAAGTAGCTGGGACTACAGGCACCTGCCACCACGGCTAATTTTTGGAG
TTTTAGTAGAGACAGGGTTTCACCACGTTGGCCAGGCTGGCCTCGAACTCCTGACCTCAGGTG
ATCTGCCCACATCAGCCTCCCAAAATGTTGGGATTACAGGCGTGAGCCACCATGCCCGGCCAA
AACTTCAGTTTATAACACAATCTTTCACGTGTCTTCTGCTTTCATTAAAAGAATAGACAGTTCC
CTTCTTTATTTCAGTTTAATAAACCATGGATTTTATTTCATGCTTTGCAAAACACAAGGGCTCAC
TGACATGCACTTCTTAAACTAATTCTGGCTGGTCGCCTGTAATTCCAGCACTTTGGGAGGCTGA
GGCCGACAGATCACTTCAAGTCAGGAGTTCAAGACCAGCCTGGCCAATATGGTGAAACCACG
TCTCTACCAAAAATATAAAAAATTAGCCAGGTGTGGTGGTGCGTGACTATAATCCCAGCTACTC
AGGGGCCTGAGGCAGAAAAATCACTTGAACCCGGGAGGCGGAGGTTACAGTGAGCTGAGAT
CGCGCCACTGCACTCCAGCCTGGGCGACAGAGTGAGACTCTGTCTCAAAAAATAAATAAATA
CAAATAATGTAAAATACGAAACAAGCAATCCTGGCAGTAGCTGCTGGAATGAGAGGAGGGAG
AGGTCATAGGGAGGTCGGGGACAATGGAGCATGGAGTTGTGTTGGATTTGGCTAAGCAGCAG
GAAGTGCAAGGCATTCCAAGCAAGAGGAGGGGGGCAGGTGGGGAGCATCTGCAAGAACAGA
AGCAGCATGAGCAACCTGGCTCGGCAGTGTGTGAAAAGGCTGAAAGGTGGCTAGAGCCACTT
CAATTTCATCCTTCAGGCAAATGGGAAATTCCCAAAGGTTTGAGTGGGGAAGCAATGCCTACA
ATGAAAGTTTGAGAGTGAAGCAGAGTGATCGAATTAAGCATGTAGGCCGAGTTCTGAAATAAC
TGCAATGTGCTGAAGATCATCCATTGGCTTCTGAATGAGTATTTGCAGTTTATTTTTTAAAATGA
TTTTATTGCCAAGAAAGATAAACACTACTGTTTTGGTACAAAAACATAACAAAATGTGTTGAG
TCCCTCTTGCTGTTTTACGCAAAGTTTTAAAAATCTACTCTTGTCACAGTGGTATCACCCCTAC
TTCTGATTTCAAATAAATGTTCTAGAGACACAGTAAGGGCCCAACAAACGCTTGTTCAACAAC
ACAAGGAGAGCCAGCTTTTAAAGTAGGAAAACAGGCCGGGCGCCGTGGCTCACACCTGTAAT
CCCAACACTTTGGGAGGCTGAGGTGGGCAGATCACTTGAGGTCAGGAGTTCAAGAACAGCTT
GGCCAACATGGTGAAACCCTGTCTCTACTAAAAACACAAACATTAGCCAGGCGTGGTGGTGC
ACACCAGTAGTCCCAGCTATTCAGGAGGCTGAGGCAGGAAAATGGCTTGAACTGGGGAGGCA
GTGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGGGACAGAGGGAGACTCC
ATCTCAAAATAAAACAAAACAAAACCAAATCATACAAAAACATTAGCTGGGTGTGGTGGTGCA
TACCTGTAATCCCAGCTACTTGGGAAGCTGAGGCAGAATTACTTGAACCCCTGGGGGGAGGTT
GCAGTGAGCTGAGATCTTGCCACTACACTCCAGCCTGGGCAACAGAGTGAGGAGACTCTGTC
TCAAAAAATATATATATTAAAAAAAAGAAAAAAAAAAGTAAACTAGGAAAACACATCAGCAG
CCTGCCAACAGACTCCCCTAGCCTCGGTGAGGGCCAGTGTTCTGGGAGGCAGATCTGAATTCT
AGTCCTAGTTCACCCACTGGCAGGCTGGTGCCCTTGGGCAGGTCGCTTCTCTGGGGCTCAGTT
TCTTCCTCTATAAAATGAGATCAAATCCCATGTTCTAAGAGTTTGTGCTCTGGAGTCAGACAGA
TCTGGGTTCTACCACTGCCAGCTCTGTGATCTTGTAGCTTCAGTCTCGTCATCTGACATGGAGA
TAACAGTAACTGTCTCACTGTGTTGTTAGGGTTTAAAGGAGATAATGTATGTGAAATGTTAGCA
AACAAGTGTTAGCTACCCTGATTTCCGGTTTCAGAGTTCTGTGGTCCCAGTTTATGCCACATGC
AGTGACGTTGTATGGTAGGCTGTGGTGTGGCACCACTTCAGAACTCAGCGCATGCACAGCTTG
CAGAAGAGAAGGCCAGAGGAGACCTAAGAAGGCTCTTCGAACACTTGAAAGACCGGCATGT
AGGCCGGGCGCAGTGACTCACGCCTGTAATCCCAGCAGTTTTGGAGGTCGAGGCGGGTGGAT
CACCTGAGTTTGGGAGTTTGATACCAGCCTGACCAACAAGGTGAAACCCCGTCTCTACTAAAA
AATACAAACATTAGCTGGGCATGGTGGCGGGTGCCTGTAATCCCAGCTACTCCGGTGGTTGAG
GCAGAATTGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCTGAGATTGCATCACTGCACTC
CAGCCTGAGACAAGAGCGAAACTCCATCTCAAACAAAACAAACAACCAACCAAACAAAACC
AAAAAAAAAACTGGCATGTAGAAGAAAAATACTTTTTCTCTACACTTCTCCAAAGAATTTAAC
TAGGCCCAGGGGAGGTGCAGTATAAATTTCTAACAATCTCAACTGTCTGCCAAATGGAATGAG
CTACTTCATATGGCAGTAGTGAGTCCTCTGTCTTTGGAGGCATTCAAATAAAAGCCAGATGGCC
ATTTATCAACAATCCATGTAAAACGTTAGATGAAATAAAACCTATATATCCAAGATCTCTTCCAA
TTCAGATTTTATGAAAGAATTTCTAAGGTCTTTGTAATGAGACATTTAGGCTGTTTCAAGAGAT
CAAGCCAAAATCAGTATGTGGGTTCATCTGCAATAAAAATGTTTGTTTTGCTTTTACAG
The "TTTCCTCATTTGGCTGTTGGATTTTAAGCAAAAGCATCCAAGAAAAACAAGGCCTGTTCAA AAACAAGACAACTTCCTCTCACTGTTGCCTGCATTTGTACGTGAGAAACGCTCATGACAGCAAAGTCTCCAATGTTCGCGCAGGCACTGGAGTCAGAGAAAATGGAGTTGAATCCTTTCTCTGCCACTCTTTGAGGAGAATCTCACCATTTATTATGCACTGTAGAATACAACAATAAAATACAGCCATGTACCACATAACAACATCTTGGTAAACAACAGACTG" sequence segment is: rhdexon10
Type 2: haplotype deletion (10,831 base pairs, complete deletion of RHD Gene)
AAACAAATACCTGAAACACCTACACTACAAAAATGCATAATCTCATTATTTATTTATTTATTTG
AGACAGAGTCTCGATCTGTTGCCCAAGCTGGAGTGCAGTGGCACAATCTCGGCTCGCTGCA
ACATCCGCCTCCCAGGTTCTAGTGATTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA
GATGTGCACCACCATGCCGGCTAATTTTTGTATTTTTTGTAGAGATTGGGTTTCATCATGTTG
GCCAGGCTGGTCTTGAACTCCTGACGCTGTGATCCACCTGCCTGGGTCTCCCAAAGTGCTA
GGATTACAGGTGTGAGCTACCGTGCCCAGCCGGCAACTAGTGTTCTCATGGGAGAAGAAGA
CTTTTTGGAAGACCCTGTCATTGCGTTTGTGAGACTGGCTCCTGCTGTCCTCCTCTCAAAGC
TATCTGAGGTTTCTGTTGCTATGACATAAATAATTAGTTCCAATTATTCTCTCAGTAGGTTCAA
AACCAAAAATTTTTATCTACTGAATGAACAAGAATACTGCTTTATGTAATAAAAACTCATTTA
AATTTTTAATATTCAGTCATTATACCAAATACTTTGAGTATTAAAGCATAAAGAATTATCTTTCT
TTCTGGAACCAAACAATGAAATAAATATCTCTTAAAAAAAAACTGCATTTGTACTCTTATAAG
AAGTTTGGCATCATAGGAGATTTGGCCTAGAAAACACTTTGTCATTTTAGAGGTGTTATCCAA
TGTTCGCGCAGGCACTGGAGTCAGAGAAAATGGAGTTGAATCCTTTCTCTGCCACTCTTTG
AGGAGAATCTCACCATTTATTATGCACTGTAGAATACAACAATAAAATACAGCCATGTACCAC
ATAACAACATCTTGGTAAACAACAGACTGCATATATGATGGTGGTCATCCAGTAAGCTAAGG
TTAATTTATTATTATTCCCTTTTTTTTTTCTTTTTTTTGAGATGTAGTCTTACTCTGTCACCCAG
GCTAGAGTGCAATGGCACCATCTTGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCGAAT
CTCCTGCCTCAGCCTCCGAAGTAGCTGGGAATTACAGGCACCCACCACATCTGGCTAATTTT
TTGTATTTTTAGTAAAGATGGGGTTTCACCATGTTGGCCAGGCTGATCTCAAACTCCTGACCT
CAAGTGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGACCATAGGCCTGAGCCACTGTGC
CCGGCCTTGTTTGCTTTTTTAACAGTTAACAGTGTGCTCATAGAAACTGCTTTGACATGACT
GCAATCATGTGCTTCATAGAAACTTAATTAGATTATACCACTAGAGTCTTCAGATTTTTATACT
TTTTTTTTTGAAACGGAGTCTCACTCTGTCACCAGGCTGGAGTGCAGTGCCGCAATCTCAGC
TCGCCGCAACCTCCGCCTCCCAGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTG
GGATTACAAGTGCACACTACCACGCCCAGCTAATTTTTGCATTTTTACTAGACAGGGTTTCA
CCATGTTGGCTAGGATAGTTTCACCAGGATCTCTTGGCCTCATGATCAGCCTGCCTCGGCCTC
CCAAAGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCCTATACTTCCCTTTTTGAATA
CCATTTGGCGTTTTGAAGAATTAACAGCTTTGTGAACGTGGCAGTGCTTGTGATTCAGGCTT
CCACTGAGACCAAGGGGAGAACCTGGTTGCAGGACAAACAGACGGACAGCGTGTGGCAGT
GTTTAAATGCTCTTCTGAAGGCTGATACGACAGCTCTCTGTGCACTGATTGTATACGCATCCC
AAGATTATATTATTGTTTTCTATTGCTATGTGTCACACTTTGCCAAACAGGATGTGGAAAATG
AATAAGCGGTTTTCTTAGGCACTTCTTAACAGACAATTGGTCAAAATGAACTCCATTGCTTA
AGAAACACATAAACACCATTTAGTCACTGAATATAGCTATATGTATGGTTGCTACTATGGGGA
ATCTTGTTTTGCCAATTTTCTTTGAAAATTCTGGCAGACCAAGGTTCTTTTTGTTTACACAAT
ACTTGAAAAATAAAAATGAACAAGCCAACAAACTACCAAGTTTTCACTTACATAAATGTAGT
TACATACAGAAAATGTGACTGTGAATTTTTTCTAGGACTTTTAAACTATAAGCACTATTTGCA
CGAAAGAGAACCAATCTATCAATTACAAACTCACATAATTTTACAGATTTTTTTTTCCCTACA
CAGCACATAAAACAGAAGGAATTTGAAGCCACCCTCCAAACACAGGGGAAGGAGGCTGTG
TGTATATCCTCATTGTCTTTCACATTCTAAGGTGGTTCCACTCAGTGACTGAAATCCTTAAGT
GTTGTATTAGTCGGCTTGGGCTACCATAACAGCAGCTTAAACTGTTGTTAAGCCACTCAGAC
TTAAACAACAGAAATTTATTTCCTTATAGTTCTGGAGGCTGGAAGTTCAAGGTGCCGGCAAG
GCTGGTTTCTGGTGAGACCTCTCTCCCTGTCTTGCAGATGGCTGCCTCCTCCCTGTGTCCTCA
TAGAGCCTGTCCTCTGCTTTTACACTTCTGGTGTCATCTTCCTTTTTTTTTTTTTTTTGAGACA
GAGTCTCGCTCTATCGCCCAGGCTGGAGTGCAGTGGCCCGATCGATCTCGGCTCACTGCAAC
CTCTGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGG
TGCCCGCCATCATGTCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATATTGG
CCAGGCTGGTCTCCAACTCCTGACCTTGTCATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGG
ATTACAGGCGTGAGCCACCGCACCCGGCCTCTTTCTCTTCTTATAAGGACACCAGTCCTATTA
GATTAGGGCTCCACCCTCATGACCTCATTTGACCTTAACTATTATTTCTTTAAAGCACCTATTT
CCAAATATAGTCACTTTAGGGGTTAGGGCTTCAAAATATGAATCTGAGGGAGATCAATTCAGT
AAATAGCAGTAGTCATTAACGGACAATATATACAAAGATAATTTCGTGATTACTGTCCTTATGC
ATAAATGTCCTCAGTGTTCCACTGCCTTTATCCAGATTTACTATCACAAAGACTTTGCTCTGA
GAAAAATGTGATTTCTTTCTTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTGTCACCCAGG
CTGGAGTGCAGTGGTGCAATCTCGGCTCACTGCAATCTCCGCCTCCCAGGTTCACGCCATTC
TCTTGCCTCAGTCTCCCGAGTAGCTGGGCCTACAGGCGCCCGCCACCCTGCCCAGCTAATTT
TTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTAGCCAGGATGGTCTCAATCTCCTGACC
TCGTGATCCACCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCC
CAGCAGATTTTTTTTTTTTTTTTTTTTTTTGAGATGGAGTCTTGCTGTGTTGCCCAGCCTGGA
GTGCAGTGTTATGATTTTGGCTCACTGCAACCTCTGTCTACCATGTTCAAGCGATTCTCCCAC
CTCTGCCTCCCGTGTAGCTGGGATCACAGGCACACGCCACCACACCTAGCTACTTTTTGTAT
TTTTAGTAGAAATGGGGTTTCACCATGTTGGCCAGGATGGTCCCGAACTCCTGACCTCAAGT
GATCCTCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGTGCCTGGCC
AAAAATGTGATTTCTTATTTCCCACATTGCCAATTCCATTTCAATTAACTATAATAGCTATGTCT
ATTGAGCACTCAAGTGTATTCTAGAAACTGTTCCTGATTCTGGGGATATATCCATGAATCAAC
TATAGTCCCTGTTATTAAGTAATCTGTAGTCTGACTAAACCATTAGAAATTTAAAAAATGGCT
ACTTTCAAAGACATCTTGGAGTTCAGGAGTCCCACACTGCGAACCATATTACCTAATAATCC
AACCTGCTTGTAATTCACTTATTTAACCAATATTTATTGAGTGCCAACTTTGAGCCTAAGATAC
AGCAGTAAACAAATGGATAAAGTCCCTGTCCTCATGAAACTTGTATTCTAATGGAAGAAACA
GAAAACAAACAGATATAGGATGTAATATCAGGTAGGGATAAATACTTTGAATTCAAACAAAA
GTATACGTAGTCAGGGTTCGCCAAAGAGACACAGCCAATCGGATACATAGATATATAAAAGA
GGGTTTATGAGTTAGAAAGGGCTCACATGATTACAGAGGCTGAGAAGTCCCACAGCAGATT
GTCTGCAAGCTGGAGACCCAGGGATACTGGTAGCATGGCTCAGTCCAAGTCCCAAAGCCTC
AGAATCAGGAAAGCTGATGATATAATTCTTAGCCCAAAGGCCTTAGAACCCCAGCGGTGAC
GGAAAGGCTGATGTAGGTCCTGGAGTCCTGAGACCCAACAGCCTGGGATCCTGAAATCCAA
GGGCAGGAATGGAAGCGTGTATTCCAGCTCCAAGAGAGTAAGACCAATTTGCCTTTCTTCC
GTTTTTGTTTCAAGCCACCTGCACATTGAGGGTGGATGGTTCCCTCTTAGTCCATTCAGTCAT
ATATCAATCTCTTCTGGAAATACCCTCACAGACACACTAACAAATAATGCCTTTCCAGTTCTC
TAGGTATTCTTTAATCCAGTCAAGCTGACACCTAAAATTAACCATCACAAAAGTTAAGGAGA
AAGAAGACAACTTGTAGGGGAGGCTGCTATGCAAGACAGTGTGTGAAGGAAGGGCTCTCT
GAAGAGGTTAATATCTGAGCAGAGACTTGAATGAAGTGAAGAAGTGAGCCATGTGGGTATG
GGGAATACAACTTCCAGGTAGAGAAGACAAGTGTGGTGTGTATCAGGGTCAGCAAAGAAG
CCATGTGACAGAGAAGGGTGGGCCAGGGAGAGACGGATAAGTGATCTAACTCCTGAGGAG
GTGGCCTGGCCAGGAGCTAGAGCATGAAGATCTCGTAGGACTTTATTCTGCAAGGTGAAAA
GCCATTGTATTAGTCTGTTCACAAACCCGAGACTAGGCAATTTACAAAAGAAAGAGAGGTTT
AATGGACTTACAGTTCCACATGGCTGGGGAGGCCTCACAATCATGGCGAAAGGCAATGAGG
AGCAAGTCACGTCTTACGTGGATGGCAGGCAAAGACAAAGACAGCTTGTGCAGAGAAACT
CCCCCTTATAGAGCCATCAGATCCTGTTAGACTTATTCACTATCACAAGAACAGCACGGGTA
AGACCTGTCCCCATGATTCAGTTACCTCCCACTGGGTCCCTCCCACAACGCATGGGAATTCA
GGATGAGATTTGGGTGGGGACACAACCAAACCCTATCATTCCACCCATGGCCCCTCCCAAAT
TTCATGTCCTCACATTTCAAAACCAATCACACCATCCCAACAGTCCCTCAAAGTCTTAAATG
ATTTCAGCATTAACTCAAAAGTCCACAGTCTAATGTCTCATCTGAGACAAGGCAAGTCCTTT
CCATTTATGAGCCTATAAAATCCAAAGCAAGTTAGTTACTTCCTAGATACAATGGGGGTACAG
GCATTGGGTAAATACAGCCATTCCAAATGGGATAAATTGGTCAAAACAAAGAGGCTACAGG
CCCATGAGAGTCCAAAATCCAGTGGGGCAGTCAAATCTTAAAGCTCCAAAATGATCTCCTTT
GACTCCACATCTCACATCCAGGTCACGCAGATGGAAGGGGTGGGTTCCCATGGTCTTGGGC
AGCTCTGCCCCTGTACCTTTGCAGGGTACAGCCTCCCTCTCAGCTGCTTTCATGGGCTGGCA
TTGAGTGTCTGCAGCTTTTCCAGGTACACGGTGCAAGCTGTCGGTGGATCTACCATTCTGGG
GTCTGGAGGACCTCTTCTCACAGCTCCACTAGGTGGTGCCCCAGTAGGGACTGTGTGTGGG
GTCTCTGACCCCACATTTCCCTTCTGCACTGCCCTGGCAGAGGATCTCCATGAGGGCCCTGC
TCCTGCAGCAAACTTCTGACTGGGCATCCAGGCATTTCCGCACATCCTCTTTAATCTAGGCG
AAGGTTTCCAAACCCCAATTCTTGACTTCTGTGCACTCGCAGTCTCAACACCACATGGAAG
CTGTCAAGGCTTGGGGCTTGCACTCCCCGAAGCTACAGCCCAAGCTCTACCTTGCCTCCCGT
CAGTCATGGTTGGGAGTGGCTGGGATGCAGGGCACCAAGTCCCTAGGCTGCACACAGCATG
AGGACCCCGGGCCTGGCCAACAAAACCATTTTTTCCTGATACCTCTGGACCTGTGATGGGA
GGGGTTGCCATAAAGACCTCTGACATGCCCTGGAGACATTTTCCCCATTGTCTTGGGAATTA
GCATTTGGCTCCTGTTACTCATGCAAATTTCTGCAGCCAGCTTGAATTTCTCCTCAGAAAATG
GGAATTTTTCTTTTCTATCACATTGTCAGGCTGCAAATTTTCCGAACTTTTATGCTCTGCTTCC
CTTATAAAACTGAATGTCTTTAACAGCACCCAAGTCACCTCTTGAATGCTTTGCTGCTTAGA
AATTTCTCCTGCCAGATACTCTAAATCATCTCTCTGAAGTTCAAAGTTCTACAAATATCTCGT
GCAGGGGCAAAATGCCGCCAGTATCTTTGCTAAAACATAACAAGAGTCCCCTTTGCTCCAGT
TCCCAACAAGTTCCTCATTTCCGTCTGAGACCACCTCAGCCTATGGACTTTATTGTCCACAGT
GCTATCAGCATTTTGGGCAAAGCCATTCAACAAGTCTCTAGGAAGTTCCAAACTTTCCCACA
TTTGCCTGTCTTCTTCTGAGCCCTCCAAACTGTTCCAAACCCTGCCTGTTACCCAGTTCCAA
AGTCACATACCCATTTTTGAGTATCTACGGCAGCACCCCACTCTACTGGTACCAATTTAGCCA
CTGAAGTAGTTGGAGAACAGAAGTAATAGACTCTGGTTTACATTGTAAAAGCTTCTCTGTGG
CTGCTGTGTGAAGAAAATATATGAGAATGAAGCCCCAAGATGAAGCAGGGACACAGTTGCA
GTGGTTAGAGTAAGAAATGCTGCTGGCTGGCACTGAAGTGATAGCCTGGAGGTTTGTGTGT
GCACATGCATGTGTATGTGTTTTACGATAGTAGGCCCAACAGATACTGTAATCCACACTTGTTTTTTTTTTTT "RHD Gene all deleted at this position"
TTTTGAGACAGAGTCTCACCTGTTGCCTAGACTAGAATGCAGTGGCACAATCTTGGCTCACT
ACAACCTCCACCTCCCAGGTTCAAACAATCCTTGTGCTTCAGCCTCCCGAGTAGTTGGGATT
ACAGGTGTGTGCCACCGTGCCCAGCTATATTTTTTGTATTTTTAGCAGAGATGGGATTTTGCC
ACATTGGCCAGGCTGGTCTTGAACTCCTGGCCTCAAGCAATCCTCCCACCTTAGCCTCCCAA
AGTGCTGAGCCACCACACCTGGCCGCAACTGATTTTTAATCATGAAATGACACATACATTTA
AAAAACCCAATACCTATAATATTCCTGGCTAGTACTCTTCACATCTATATCATCAAAAACAAA
GAAAGTATGTGAAACTGACACAGCCAAGGGGAGACTAAGGAGACATAACAATTAACTGTAA
TGTGGTATTCTGGAGGGGATCCTGGAACAGAAAAAGACATTAGGCAAAAAACTAAAGAAAT
CTGAATAAAATGTGGATGTCAGTTAATAATAATGTATCATATTAGTCCAGTAATTGTAACAAAT
ATACCCAATAATGAAAGCCATTAATTATAGGGAAAATGGAGGGGTTAATATGGGTGGCTGGC
TTTTGCTATTTCTAGCAGCTCCATTTTATCTACAAAAGACAAACATTCATTAAGTCCCAAAAA
GGTAAAGAATGACAAATTAAGCATGTATCTTATTAGTAAGAGTAATATAAAGATGCTCACTCA
TATTTATAAATATTTGACAATGATGTTAAGGCCAGAAAAGAGAAAAAAGGGTAGGGGCAAA
AAACGCAAAGAGAAAGGAGTTAGTATCTTTTCTCCCGCACTCATTAGCTATTAAAAGAGGAT
GTTTGTTTAAAGCTGCTCAGAGCTGGTAAACTAATGTTAAGTCACTAACGGGAATTTAAAAG
GTTTCATTAAGAACTGCCTGCACTAGATTCCTCCACCCTGAGACATTAAACAATCACGATAA
ACCTCCTGAGTGGTAAGAACGTGTCCATTTAAAAACAGGCTATAGATTGTCATGCAGTTTTAT
CTACTAATCGGCTAATGCACCGCCAAAAACAAACAAAAAAACCCAAAGGGATGAAAGTTTC
ATCCATCAAAGGAAACAACAGTCACCTTGGTTCCCATCCCACTCATATACTGCCGCCGTACA
TGTCAATCAGATGAACCTGTGCGTATCTCTTAACGACAATTGACCCACCTTTTTAACTGAAG
TGAAGGGGGGTTCTGCTCCGCGACCACTTCCTGGATCTCTCCCTTCACCCTCTGTGTTCTTT
CGGGTGCACCATCGGGTCAAAGCCGCAGCAACGCCGTCTCTGTGTGATCGCATGTGCCCTT
CTGCACACGACCTTCCCCCGAGAGTGACCAGCTACCGGACAGGCACCAAGGAGGGCTACC
GAGCACCTCCCGGACCGGCGGCTGCAGGATCGCGAGCGCCTCCGCTAGGGAGACCGCACG
TTGCGCCTGTGCTTCCTGCGGTGGCGCCTTCTGCAAGGAGACCTCGACCCTGCTCCCTCTCC
GGGGCTGGATCTGACTCCTTGACGGTGATTCCAGACGCGAGACCCAAACTGACGGCTTCTA
GAAGAGGGGCGAGCCCGGCCGCAAGTCTTTCACGTAGCTAAGTCATCGTTGCTTCCGGCTT
CTTACCGTTCTCCCCTTTGTAAACGGTTACCTCCCGAAAACCCAGGCTCTCCTCCAACAGTG
GTTCTCAAGCGAGGCGATCTTCCCCGGGAGGGGATATTTGGCAAAGTCTGGGGGCATTTTTG
GTTCACTGGGGCTGCTACTTGCATCCACTGGGTAGAGGCGGGGGATGCAGCTACACAACCT
GCGAAGCACGGGACAGCACCCTCCCCAACCCAGACAGAATTAGCCGGCCCAAAACCTCAG
TAGTGCCCAGGCTGAGAAACCCTGCCTTAAACAAACAACAAAGAAAGGCCAAGTCCCATA
AGTGGGTCACCGCGCCGAGACTGGGGTCCACGGGACACCCCAGCCACGCCAAGCCGGGAA
GTCCCCGCCTCCTGGAGCTGAACCCGCCCCTCTCCCAGAGGTGGAGCTGCGGGGGGCGGG
AACAGGCACGGAGAAAATAAACAAGACTAAAAAGTCCTGAGTAGCGCTGTGTGGCCGCAA
ACCTGAACCCACCTTTTGCACCACGCGGGACCCGGCACTCTTCCTGCCACCCACCCCTGAG
AGGGCTGCGCGGCCGACCCCAGTACTAGAAAACACTCGTCACCTCACTCAAGACGGGTACG
AAGGCCAACGGACGCCTTCCTTTAGAACGCTCAGCACACAGAGCAACTTCTCACGCCTACT
CTCAAATGGCGTACTCCAAACTAGCACTCCCGACGTCCAGCTGTGAACCCAGAGCGGCGGA
AAGCCCCTGAACCCAGCGCCCGGGCATGCGCAGACGCGTTGTTGTGGTGGGCGTGGCTCCC
TCCGGACCCGGCGCCCCGCCCTCCGCCCCGTGTCCGCATGCGCGACTGAGCCGGGTGGATG
GTACTGCTGCATCCGGGTGTCTGGAGGCTGTGGCCGTTTTGTTTTCTTGGCTAAAATCGGGG
GAGTGAGGCGGGCCGGCGCGGCGCGACACCGGGCTCCGGAACCACTGCACGACGGGGCTG
GACGTAAGTGTCGGGCGGGGAGTGGGGGTAAGAGAGTAGCGGCCGCAGGCTGGAGAGGCC
TCGGAGCCGGGGCCGTCTGACGGGCTGGTGCCCACAGCACAGGCTCGTTGCCGCCACCCTC
CCGGAAGCCGCAGACCCGGGCTGAGCCGCCGCGGACCTCTCCTCTCCTGGGTCCGGCGGC
GCCAGGAGAAGGCGACGGCGTCCAGACCCCGCCAAGACGGAGAGGGGACCGCGCCCTCC
CCCGGGACACCCCAGGATCCCTGGAAAATCCCCCGATCCCCCACTTCACAGCTGGACAAGC
CGAAGCCCCGAGAAAGGAGCGTGACCCGGCCAGCTCCCTCAGCGAGGGGTGCCCCGGCTA
AAGGTGGCTCCCCCATCTTCCAGCGGCGTCCCTCGAGACCTAAAAGTCCCAGAGTTTGGAG
GGCGGGGTCTGGCTTGAGCGTACCAGTCGTCGTGCAAAGAAAAATGGATTCGGGGTGGGG
CCATTGGGATGAACCGAAAATAACACTTATTGAATTGCAGTTCTTCCCGGGGAAGAATCAAA
TGAGTGAATTTCCTAAATTGAACTTGTCCCAAAGGCATCTTAGCATTGGCTGTAATCATATAT
TTTAATATTTAATTTTTAAAAGAGAAGTTAAAAATGGGAGACGTTGCTCTATTGTAGGGTGTC
ATATTTTTGGACAACCATAATTGCTGGCTACAAAGTATCTTAAGGAAGTTAAGTTCGCTGTAG
GAGTTTGGGTGTTGTTATTCGCAAGTTATATGTATTTCAGGCTTTTTCATACTCTGAAATAGAG
ATA
ACATGGCTGTATCTCAGGTCTCTACTGCTCGGCTTACA
Type 3: haplotype recombination (recombination of RHD gene exon3 to exon 9 with RHCE gene)
CACGCCAAGCCGGGAAGTCCCCGCCTCCTGGAGCTGAACCCGCCCCTCTCCCAGAGGTGG
AGCTGCGGGGGGCGGGAACAGGCACGGAGAAAATAAACAAGACTAAAAAGTCCTGAGTAG
CGCTGTGTGGCCGCAAACCTGAACCCACCTTTTGCACCACGCGGGACCCGGCACGCTTCCT
GCCACCCACCCCTGAGAGGGCTGCGCGGCCGACCCCAGTACTAGAAAACACTCGTCACCTC
AATCAAGACGGGTACGAAGGCCAACGGACGCCTTCCTTTAGAACGCTCAGCACACAGAGC
AACTTCTCACGCCTACTCTCAAATGGCGTACTCCAAACTAGCACTCCCGACGTCCAGCTGTG
AACCCAGAGCGGCGGAAAGCCCCTGAACCCAGCGCCCGGGCATGCGCAGACGCGTTGTTG
TGGTGGGCGTGGCTCCCTCCGGACCCGGCGCCCCGCCCTCCGCCCCGTGTCCGCATGCGCG
ACTGAGCCGCGGGGGTGGTACTGCTGCATCCGGGTGTCTGAAGATCCGATGAAATAACATAT
GCAAAATGATTGGGTCCGTGATTGGCATTCCAGAAATGGTAGCTGTTATTCAGCCAACAAAT
ATTTATTGAGCACCTACTATGGACTTCCCTGGTGCTGAGGATACAACAGCAACCACAGCAGT
CAAAAGTCCCTGTCTTCATGTTGCTCAGATTCTCATAGGGGAAAGCAAATAATGAACAAATA
CACGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGTACTTTGCGAGGCCAAGGTGGGCA
AGTCACCTGAGGTCAGGAGTTCGAGACCAGACTAGCCAACGTGGTGAAACCCTGTCACTAC
TAAAAATACAAAAATTAGCGCGGTGTGGTGGCTCATGCCTGTAGTCCCAGCTACTTGGGAGG
CTGAGGAAGGAGAATCGCTTGAACCTAAAAGGCAGAAGTTGCAATGAGCCAAGATCGTGC
CACTGCATTCCAGCCTGGGTGACAGAGTACTCCGTCTAAAAAAAAAACCTAAATACACAAG
TAAAAATATAGACCTCGTCAGATGCTAGTAAGTGCTGTGAAGGAAACTAAAAGGGGAACAC
AAGGAACCCTTGTCAAGGGGAGAAGAAAGGGGAGTTGATGCTGTCCTTTTAAATAGGGCA
GTCAGAGGCCGGGCACAGTGGTTCACACCTATAATCCCAGCACTTTGGGAGGTTGAGGTGG
GTGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACCTGGTGAAATCCTGTCTC
TACTAAAAAAACAAAAACTAGCCGGGTGTGGTATCACGCGCCTATAATCCCAGCTACTCGGG
AGGCTGAGGCGGGAGAATCACTTGAACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATTG
TGCAATTGCAGTCCAGCCTAGGCAACAAGAGCAAAACTTCATCTCAAAAAAAAAAAAAAA
AATAGGGCAGTCAGGGAAAACTTTCCTGAGAAGGGGATGGTGGAGGATCCAGGGAGGTGA
GGTGGGGAGCAAGCCAGTACAGTTGTTCCTTGACTTTCGATGGGGTTATGTCCTGATAAAGC
CATGGTAAGTAGGAAATATTGTAAGTCAAAAATGCATTTAATACATCTAACCTACGGAACATC
ATAGCTTAGTCTCACCTACCTTAAACATGCTTAGAACACTTACATTAGCCTACAGTTGGGCAA
AATCCTCTAACACAAAGCCTATTTTATGATAAAGTATTGAATATCTCATGTAATGTACTGAGTA
CTGTACGGAAAGTGAAAGACGGAGTGGTGGGATGGGAACTCTAAGCGCGGCTTCCACTGC
ATGTGTGTTGCTTTCGCGCCATCATAAAGTTGAAAAGCGTTAAGTCAAACCATCGTACGTCG
GAGGCCATCTGTATCTGGTAGGAGGAGTGTTTCAGACAGAGAGAACAGCAGGTGCACAGA
GTGCTTTTTTCCCAGCATTTTATTATGAAAAATTTCAAACATCTACCAAAAAAAGTTGAAAG
ACTTGTACAGTGAAAAGCCATACATCTCACAGCTAGAATCAACAATTAACATTTTACTGTATT
TGGTTTTTGACTTATCTATCCTAGATCCCTTGTGCTTTCTGTAGCAGGTGACCTGCCTTGAAG
ATTTAAAGACAGAATATCAGGAAATGTAGTCAGAAAATGGGGCCTTTTATAAGAGTCAGAGG
GGAAGAGCAAAACCTCTGCTTTTGACAAATCTGTTGGGAGAGGCCAACTGCAGGGATACCT
CCCTTTTTTAATGAAAGCATTTCTGTTCTGCGAGGAGCGGGATCCTCTTGTCAAGCAGTCAG
TCCCTGCTGCTTCCTTACTGGGGCAGGATCAGGACGCACAGGGATTTGGAGTGCCTTGGAA
CCAACCACCACCCACGCCGTTTGCCAGCTGGTAAACATGCCCATCAGGTCCGGGGGTTGGC
ATTGCCTGGACATCTTTAGTGTTCATCTTGCTGACATCTGGTGCCCTCGGGCAGGTAGGTGC
AGTTGGCTGCCTGGTTTACAGAGCTTGTACTGGGCCCAGGTTAGCAGAGGTCACATCCATTT
ATCCCACTGCGCAGAGGAGTTCCTTCTCAGGAAACCCAGTTTATAAGAAGTACTGACTGCC
AGAAATAGAGCAGAAATGAGAACCAGGAGGCAATTGTGAGAGGAATGGAGACTTCTGACC
TCTGGGGATTGGGGTACCCTCCCCCTTAATTGCTGTTGGGGTAGCAGAGGGCTTAGAAGCCC
ATGTTCCTAGACTTTTAGAATTGGAAGAAGACTTAGAAGTAATCTAGGCTGGGGGTCCCCAA
CCCCCAGGCTGTGGCCCGTTAGGAACCTGACCGCACAGCATGAGGGATAGGCCAGCGAGC
ACTACCGCCTGAGCTCCGCCTCCTGTCAGATCAGCAGCGGCATTAGATTCTCATAGGGGCAC
AAACCCTATTGGGAACCGCGCATGAGAGGGATCTAGGTTGCGTGCTCCTTAGGAGAATCTAA
CTAATGCCTGATGATCTGAGGTGGAACAGTTTCATCCCCAAACCATCCCTCCAACCTCACCC
CGGTCCATGGAAAAATTGTCTTTTACAAAACCCGTCCCTGGTGCCAAAAAGCTTGGGGACC
CCTGATCTAGGCTACAGTTAAGTGGTCAAACACCCAGGTCCTGAAGTTAGGCTGCCTGGGTT
TAAATCCCAGCTCTACTGCTTACTAGCCCTGTGACCTTGAGCAAGTCACTTAGTTTTTCTGTG
CCTCAGTTCACTCATTTGTAATAAATCCTAATAGTACCCATCCCAGTGTCATGAACTAAGTTC
ATATATGTAAAGTGCTTAGAATGGTGCCTAGCAAGTACTTAATAACAGTTAGCTCTGAAAATG
TATAAAGCAAAATTAACCAATGTTTTAGTGGTTTGCAGCCAACTTTTTTCTATGCGTGTGCTA
ACATATTATTTTATAAGAGTGGGAATATATTGTACATGCTGTTATATAACTTGCTTTTTCACTAA
ACAGTCTATCCTCTGTGTCAGTTTTGATAAAAGCGTTTTCCTCTTGCTTTTCCTGCATATGTTC
AGAACCATCATATTGGTAGCAAGTTTCATGTCCTGTAGTTTTCTTAACCAACCCCCTGCTAGT
GGACATTTAGGTTAGTCTCAGTTTTTTCCTTCTGTAAATAAAGCTGCACTGAGCAAGAAGTG
ACTGATGCCAAGTGACTAGATGACCTTAGGTATGACCTCTCTGGGTCTTGGTTTCTTGGTCTA
AAAACAAAATGACAGGATTCGACTGGGTGATTAAAATCTCCTCTGATCTACATAGGAATTGT
TTTCAAGACATTTCTGCATTCCTCTAGTGACAGGGTGCTCACTACCTCATGAGTATTTCAGTG
GACAACTGTAATGGTCAATAAAGTATCCACTTTCCACCTCCCTGCAGCTCCTGGCCCTGGCT
TTATTCTCTGGGGCTCCACACATTCAGTTTACACTCAGTGGCCAGTGGCTGGGACCATTGTA
GAAAATAAGGAAACTCCAATTCCTTCCTTCTTTTCTTCCTCTTTCATCTCTTCCTCCCTCTCTA
CATCCCTCTCTCTCTTCCTTCCTTCCTCGACACTTACCATGTACCAGACCTTCTGCCAGGCAC
ATGGATGGGAGCACAGGGGAAGTTGGCTGCAGGGTTAGAACTAAGTCCCAAGCCCCCTAAA
GCTCATGCCAGGGGACTGGACTGTCCAGTACTGAGGGATGGGGATGCTGAGGCTGGTGGCC
TTCCTCAAATGCACTGTAGTGCCCCAGGCAGAGTCCTGGGCTGCCCTGTGAGGAGGTGACC
AGAGGTAGAGCAACTTCACCCTAAGGCTGGATCAGGATCCCCTCCAGGTTTTTACTAGAGCC
AAACCCACATCTCCTTTCTCTTCTGCCACCCCCCCTTAAAATGCTTAGAAACACATAGATTTA
AATACAAATTCAAATGTAAGTAATTTCAACTGTGTAACTATGAGGAGTCAGTTCTACGTGGG
TCCTATCTGTATCCTCCCCAGGGCTCAGCTCCATTCTTTGCTTTCATTCATTCTCATTCAATAC
ATTGTTGTTAAGAGCTCACTGGGTGCCCTCTCTGTCATGTAGTAAGGTTTTAAAAAGAAAGC
CTCTTCTGAGCTTCAGTTTCCTTATTCATAAAATAGGAGTATTGATCCGTTCCTTGCTTTTCTT
ACAAGGATATGCTGAAGATGACTGAAGTACAGAGTAAAGAAGGATTATGTTTGGGTGTCAA
AGGAATAGAATGCCCTCTTTCAAACTGAGCACAGCAGGAACCTGTAACAGGAACACAGCA
ACTTGTTGAATGAATGACAATATTGGAAAACATACATTTCCTCCCCTCCCCATCATAGTCCCT
CTGCTTCCGTGTTAACTCCATAGAGAGGCCAGCACAACCAGCCTTGCAGCCTGAGATAAGG
CCTTTGGCGGGTGTCTCCCCTATCGCTCCCTCAAGCCCTCAAGTAGGTGTTGGAGAGAGGG
GTGATGC
The "CTGGTGCTGGTGGAACCCCTGCACAGAGACGGACACAGGATGAGCTCTAAGTACCCGCG GTCTGTCCGGCGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTCTCATTCTCCTCT TCTATTTTTTTACCCACTATGACGCTTCCTTAGAGGATCAAAAGGGGCTCGTGGCATCCTATC AAG" sequence segment is: rhdexon1
GTGAGAGTTCATTGGAAAAGTGGTCACAGGAGCAAATAGCAGGGGCAGGGGCGGGGGAGG
CCTGTGGTTCTCCAGGGGCACAGATGTTCCTTTCTACAAAATCCCAAGGAAAAAGATTCCCC
CATCTTCTTCCGTAGATTGCACCGAAATTCAGCCAACAATGTAAGCTTTCCTTTAGAAGCAG
CCTGGGCATGCCCTCTTCTGTGAAGCCTGCCTTGATTTTTCAGCACAGTGAGAGGCATCCTC
TTTGGTGTTCCTCAAATTCCCTCTACCAAATGGTCTTCATAATTCTCTGCTTCTCTGCTTCCCC
TTCTCTCTCCTCAGTGGCAAGGAATTTTTTTATTTTTATAGATTTAGGGGATACAAGTGCAGCT
ATCTTATGCAAGCAATTTCATGTTGTTGGGTTTTTGGTTTTTGTTTCCTTTTTGTGGCCTCTCG
CTCATTTCTTATTTCTTTTTGAGGCAGGGTCTCACTCTGTTGCCCAGGCTGAAGTGCAGTGG
CATGATCATGGTTCACTGCAGCCTTGACCTCCTAGTCTCAAGCAATCTTCCCACCTCAGCCT
CCCAAGAAGCTGGGACCACAGGAGGGCACCACCATGCCTGGCTAATTTTTTTTTTTTTTTTT
TTTGGTAGAGATGTGGGTCTCCCTGTGTTTCCCAGACTGGTCTCAAACTCCTGGACACAAGC
GATCCTCCAGCCTCAGTCTCCCAAAGTGCTGGAATTACAGGCGTGAAGCACTGTGCCCAGC
TCTCTTGCTCATATCTATACTAGTTTTCTTTTGGAAGCTTCAGCCTGTTGCTACCCCCCACCCC
CACCCCCACCGACCCCAGCTTTCTTCTCACTTAGGGGCTGGGAAGTCTGCATGCTGTCTATA
AATCCAGAACCAGAAGGTATGGCTGAAGGGGAGGGTAGGATGATGGTTATTTTATATTCAGC
TAAAAATATTCCCAGACTGTGATGAGACAACTGTAAATAAGACAGATGTCCACAATGGTGTG
ACTTTGCTTTTTTAAAAATATTGAAATGAGTTTCAGGCATCTCAGTGGGCTGATAGGTTGTTG
ATAATAGACAGGGCCTCCTTGAAGAATGTCCCTGAGACAAAGTTGAAGCTTGAGCCTGGTT
GAGTCCTTGCTTGTTCCTAGGTTGATATGAACGGCTAGTTAACTGGAAGCAAAGAGAAGTCA
TCCTGGGGGCCATGGCAGTGACAAGTAGGACTTAGGGAGGGAAGCCCTTATACCATTTAAG
GTGCTGGCCCAGAGAGGAGCCTTCAGTGACAGACAAACAAGAGCTGGCACAATTTTAATTC
ACTTCAATTTACTCTAATTCATTTCAATCCAATACAATTCAATGCATTCCATTCATTCAACCAT
GTATGACATCCAATGTGGGATCCAGACTCATGATGATTAGAGCTGATATTTATGAGCACTTAC
TATGTACCAGGCACTATTCTACATGCTTTACATTGAACCCTCACAATAACCCAATGAGGTGGG
TACTATTATGATCTTCGTTTTTCATATGAGGAAACTAGGCATATGGATGTTGAGTAATTTGCCC
ACGGTCGCTCAGCTAGCAATAGCACAGCGTATTTAAATTTAGCCACCCTGGATTTAGTTTCCT
TACACTTAACCATTATGCATCATGGCCCCATTTTACAGTGGGCTTGAGTCTTTGTCATATAACC
CAGTAGGTTAGCAGCCACTATTCCAACCCTGTAGATTGACTCTAGGGTCCATGTTCTTTACCC
CTGCACCGTGCTACTAACGTAGGTACAAAATGTCCTCAGAAACTCACTTTATACGGAAGCTC
AGAGGAGGGTCCACAACCCAGGCAGGGGAGACGATGGTGTCAGGGGAGGGAGGTGACTG
CCCAGCCAGGTCTTGAAGGCTCAGTAGGAATTACCTGTGGGACAAAGGAGGGTCATCCAAG
TGAGGGCACAGTGGGTGCCATGGCGTGCACACACAATAGAGCAGACTGAGCCTGGGCTTA
ACATTGCATTGCCCTGGAGCCTAAAAGGGGAAACAAAGGGCCGGGCGACGTGGCTCACGC
CTGTAATCCCGGCACATTGGGAGGCCAAGGCTGGAGAATCACCTGAGGTTAGGAGTTCGAG
ACCAGCCTGGCCAACATGGCAAAACCGCATCTCTACTAAAATTATAAAAACTGGCTGGGTGT
GGTGGCACACGTCTATAATCCGAGCTACTTGGGAGGCCATTACACTCCAGCCTGGGCGCCAG
AGTGAGACTTCATCTCAAAAAACCAAACAACAAAAACAACAACAAGAACAACAAAAAAA
CAAAGAGGAGAGCAGGGACTGGGTGTGGTGACTCATGCCTGTAATCCCAAACACTTTGGGA
GACCAAGGCAGGCAGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTA
AAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGATGTGGTGGCACGTGCCTGTAGTCC
CAGCTGCTTGGGAAGCTGAGGGAGGAGAATTGCTTGAACCCAGGAGGCAGAGGTTGCTGA
GCTGAGAACATGCCACTGCACTCCACCCTGGGTGACAGAGTGGGACTCTGTCTGAAAAAAA
TAATAGTAATAAATAAAAATAAAGAGGGAAGCAGCGGGTGGCAGACTCACTGGGCTGCATA
CGAAGTTTGGCTTCAGTCTGAGGTCCGAATAGTAAACAGCAGCGAGACAAGTTTGGGTTTG
GGTCATGGAGGAAGCCATGCCAGGGCTGGTGTTGGGCACAGGGAAAGGGGCATGGCTTGA
GACACCAGACCAGCGTGGAGGCTGTAGTGTAGTATTGACCTGAGGACTTCAACATTCTGAT
GGTGTACACACGATTTTTTGAGCATGTACCATGGTTATATATTACACTTTAAGTATTACTTTAA
GTATTACTACATTAATATATTTTGTATGTTACAATAAATACATACAAATTAGGAAAATTGAAAG
AGATCAAAATGAAATATATAATATTTTCAAATTACTAATCATAATGGTGTCAATCTCCAGGCAG
GGTCCATTGCTACAGTTGACGATAGTGGATGAAAATTCACTCCTCAGAGTCTTCTTGATAATT
TGAAATTGTCTTGATTGACTTGTCAGATCTGATTAGATCAACATGTTTTAAATCTCGAATGTG
ACTGACAGCTTGTACGAGGAGAAGTTTCACTCTGCCTTTTCCCTTTTGTTCACTTGACTGCC
ATTATTTCTATGCTTCCAATCTGTGTTTTTCTGCACGAGTTGGTTAAGCCATTACTTCATTTTG
TGAAAGTTTGTTGAGTTAAACTTAGGTAACTTAATCTGTCAATCCACTTAATTGAATTCAGTC
CTGGTAAACTATAATAGATTATTCAAACCTGCCAATTCTAAAAAGACATTTTGAGACAATCAG
GAAATCTGAATATAGCATGAATATCTTACGATATACAAGGATTATTGTTAATTTTGTTAGGTAT
GATAAAAGCATGGTGGGTTGTTTTTGTTTTTGTTTTTTAAGTCTCCATCTGTTAGAGAGGCAC
ATTGAAATGGCATGATATCTGGGGTTTGCTTTTATGCCAGAAAAAAGAAAAAGTACAGAAGG
ATTATAGAAACAAGATTGGTCTCATGTGACAATCATCAGAGTTTGGAGATGGGCACGTAGGG
TCATCGTGCTGTTCTCTCTGTTTTCGTATATGCTTTAAAAGTTCTGTAATAGTTAATTAAAAAA
AAAAAAAAACACCCTGGCTGAGCATTTAGGGAGGCCAAGTGGGGAGGATCGCTTAAACCA
AGGAGTTCAAGACGAGCCTAGGAAACATAGGGAGACCCCCCCCATCTCTAAAAAAAAAAA
AAAAAAAAAAAACTTTAAAATTTAACCCAGTGTGGTGGCACATGCCTATAGTCCCAGCTACT
CAGTAGGCTGAGGTGAGAGGCTTGCTTGAGCCTGGGAGCTTGAGGCTGCAGTGGGACGGG
ATTGTACCACTTCACTCCAGCATGGGCGACAGAGCAAGACCCTGTCTCAAAAAAAATAAAA
ATATTTGAGGTGAAGCGAGGCTGTAATAACAAATTTAAAAATATAAATAAAACATAAAGGCT
GGGTGTAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAAGCAGGCAGATCACG
AGGTCTGGAGATGGAGACCATCCTGGCTAACACGATGAAACCCCATCTCTACCAAAAATAC
AAAAAAATTAGCCGGGTGTGGTGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGG
CAGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTTCAGTGAGCTGAGATTACGCCACTGC
ACTCCAGCCTGGGCAACAGAGCGAGACTCCGTCTAAAAAAAAATGAAAATAAAAATAAATG
AAACATAAAACCCTGCCATTAGTTGCAATATGAAGAATATAGAGAAATGCATATCAAATCCTT
CTCATTGGACCAATATTCCCTTAGGGCACCTTCCAAAGCTAGGAGACTCAAGGCTGTATGAC
ATCCTGAGCAAGTGAGGGGTGGCTTCTGGGTGAATCTGAATATTAAATATTTGCAGAATTGA
AAACTTCACAAAGTACCTTTAGAGATAGAATAGCCTAGATCCATGTTTCTCAAAGTGTGGTC
CCCAGACCTGCTGCCTCAGCATCTCCTGGAAATTTAGTAGAAATGCAGATTCTCAGGCCCTA
GGCCAGACCTACTGATCAGAAGCTCTGGGCCTGGGGCCCAGCAGTCTGTGTTTTCACAAGC
CCTCTTGGTGATTCTTCTGTGCATGAAAGTTCGAGAATTCCTGGAGCTAGACTGATTCAAAT
CTTGCCTCTGTATCTTAGAGACCTTGGGCAGATTAGTCAACCTCTTTCTGCCTCTGTTTCTAC
TTCTGTCAGAGGATGATAGTACTTGTTTCATTAAGTTGTTGAAAGGATAAATGAATTGACACA
CATAAAGAGTATTAGCTTTTATTATCAAAAGCTTTTTTTTTGAGACAGAGTTTTGCTCTTATTG
CCCAGGGGAGTGCAGTGGTGCGATCTTGGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGT
AATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCACCACGCCCGGCT
AATTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTGGTGAGGCTGGTCTCGAACTCCCA
ACCTCAGGTGATGCACCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACC
GCGCCTGGCCCAAAAGCTTTAATTTCTTAATTTTTTAAATAAAATAAATAAAACTAGAATTGC
TTGTTTTCTTCCAGCTACCCTGGTGATTGTATTGAGCATTTTCTGGGGTGTGTGTTCTTTGCT
GTAATGACTACTGGTCTGGATGACCTGTGATGAGACCAGATGGGCAGGGGCAGTGGAGGAG
ATTCTAGAGATATTTAGGAGATAAGTCAGCTGTACTTGATGAAAAGAGTGGGGAGTTAAGGC
TGGCTGCAGATGTATGATTTGGCATAGAGAGGTGCCAGTTCCTGAGATGAGAGACAGAAGG
GGAGGGACAGGTTGTGAGGATGAATGAACAATGATATGTTCATTCTGGGCTTGGAGTTAAG
GGGCCTATGATATGCTTAGGGGAAGCAGAGAGTATCAATTACCTATTGCTGCATAACAGCCA
CCCCAAACTTAGTGGCTTAAAATAGTAACCTTTTAATTTACTCATGATCATGATTCTGTGGTG
CAACAACTGGGCTGGGTTCAGCTGGGCAGTTCTTCTGTTAGTTTCACCCAGGGTCATTCATG
CATCTGCAGTTTGGGGTGGGATGGCCTCAGATGACCTCATTCACGTGTTTGGCAGTTGGTGA
TTCACTGGGGGCCATTACTGTAACAATCGCCTACCAGGCAGAGCTTCCCTAAGGCTTCCAAA
CTAGGAGACTATCCTGGGTCCTGTGCTGTGGATACCACTCAGTCCCCCATCCCCACCCCATAT
TCCTCAAAGGCAGAGAGAGGGGCTACTAGAAGACAGAGGAGTTTTCCCAGTGACATGTAA
ACACTCCAAACCCTGGCACCTTCCACACTGCAGCTTTGGTCTGCCCCTTTGGGAAATCTCTG
TTTTTCTTCCCAGGCTGCTGGAGGGGTGAGAGTCGCCGGTAGAGTAGAGGCTGTGGGCGAG
GAGGTGGCGGCCTCCTGAGGCTGCAGTGGTCTTTCCAGGCAGCAGTGGGAGCACAGGGTG
GAGGTCAACCCTAGAGCCTGGGAGAGTGAAGCTGGGTGTGACTTCAGAGCTGTTGGTGCT
GAAGTTTCTGCAGGCCAGAAGGAGGGGCAAGAGTGGGAGGGGGCGCAGATCCAGAATCAC
GGAGGCAGCTGACCGGAGGAGGCAGCTGCCCAAGGGGATGGACTCAGAAGGCCAAAGTG
CTGTTATCCAAACGAACTCTTTGCAAGTGGTCTCTTTGCAACAGGCCTGGGGGAGAGCAGT
CTTGCCTAAAGTCACACCGCTAATCAGCGGCCGGCACGGGGTAACAGTTACTAACACTCACT
ACGTACCCAATGCTGGGCGAAGTGACTTGCATGAGCCAGCGAGCTCAATGCTCATGGCAAT
CCTCTGAGCAGCTGGCATTGTTTCATCTCAATTTTACAGCTCAGGAAGCTGGGACACAGAGG
AAGAGCCAGGCTCTGAACACTGACAACCTGATTGAGAGACCCACACTGTTCATCACCGTTA
CGCTATATATGCTGTATAGAAAGGCAGGATGGCATAATGGTTAAACCTAGGTAGGTAGGGTTT
GAATCCTCCTGCTACCATTTACTAGCTCTGTGACTTGGACTAGTTATAGCACCTCTCTGTGCC
TCCCTTTCCCCATCTCTAAAATGGGGATAATAAATCGTACCTCCTACCTGAGGCTGTTGTGGG
CTAAGTCTGTAAGGCACGTAGAACAGTGCCTGGAACGTGGGGTACTGTCTATCTGTGTGCCT
GCTGTTACAACAATGGTGAGTATTGCCTTATCTCTCGCTGCTGAACTACCAGGTTAGACTTCT
TTCTGCAAGTCATGAGGCTTTCATAAACTTTTCCTGAAGGCTTTCCGTAGAATGTACAATTCC
CCTCTGGGTCCAGGCATGGGCGCCCGGGTAGCACATCCACTTCTTATCACCCCTGAACACCT
TAGAGCCCATCAGCTTATCAAACCAGCAGCTGATGTGAGTGCAGAGCAGACTGTGAGAGGT
GGAGGCTGATACCAGTGAGGATGCTCCAAGCTGGGACCCAGCCCTGAAGCGGGAGCCCAG
ATAATGGATGGGTGGAAATGGGCCTGGAGCCCAGGAGAAGTGGGAGGATGAGGGGGCAGG
GGGAGGAGAAGCCTGAAATCAAATGTTATTTCCTGACCAGTTTGGGGTGCATGAGCTCTGTC
AACAGCTCATGGAAACTGCTGCCCTAATTTCATCTTGTTGGCTGAGGCACAATTCCTCTCTC
AGGGACAGTGTAGAGCCTTGGGGAGGAAGGCCCTGAGCGCGTATACCTGGAATCAGGGAAT
CGGGATCAGGGGCAGCAGCTGTGCCCAATAAAGCCCCCACCCAGGATCCTCTGACTTCCTC
ATCTCTTTTTTTTTTTTTTTGAGCTGCAGTCTCACTCTGTCATCCAGGCTGGAGTACAGTGGT
GCGATCTCGGCTCACTGCAACCTCAGCCTTCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTC
CTGAGTAGCTGGGATTACAGGCATGCGCCACCATGCCAGGCTAATTTTTGTATTTTTAGTAGA
GACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACTTCAAGTGATCTGCCC
ACCTCAGCCTCCCAAAGTGCTAGGATTACAGACATAAGCCACTGTGCCTGGCCTTTTTTTTT
TTTTTTTTTTTGTAAACAGGGTCTCCCTCTGTCACCCAGGCTGCTGGAGTGTAGTGGTGTGA
CCGCAGCTCACTGCAGCCTTAACCTTCTAGGCACAAGCCATCCTCCTACCTCACCCTCCTGA
GTAGCTGGGACTACAGGCACTCGCCACCACGCCCAAGTAATTTTGTATTTTTTGTAGAGACA
AGGTCTTGCTATGTTGCCTAGGCTGGTCTTGAACTCCTCAGCTCAAGCAATCCTCCCTCCTT
GGCCTCCCAAAGTGCTGGGATTGTGCTGGGATTACAGGTGTGAGCCACCATACCTGGTCTG
ACTTCCTAATCTTTAGGGCCCCAACTCTGCCCTTATCCAGGCAACTCTCCTCTCCCCATCTTC
CACTAACTTCTTTGGAATATTCCAGAGCTGTAAAAGCCTTAGAGAGTATCAAGTCCAACTCC
TATGTGTTACAGACAGGGAAACTGAGGCCTAAAGAGGGTAATGGACTTGCCTAAGATCACTT
AGTGAGGTGAGAGAAGAAAGAGCTAGAGACAGCCTAGCCTGTGCAAGGACATAGTTCCAG
GCATTCAGAGCTGGGCTCTGCTGCCGGCATGTTTGGGGCCTGGTAGTTAGTTCACTGCTGAA
CTACCAGGTTAGATTTTCTTTCTCCAAGTTGTGGAGCTTTCATAAACTTTTCCTGAAGGTCTT
CCTTACAATGTACAATTCTCCTCTGGGCCCGGTCATGAGCGCCCCTCACAGGCTCTCTCTGG
TCCCCTTCTGTAAAATGAGAGGAAAATGGAAGAATTGCTCTACTCATGGAATCTTCAATAAG
TCTGGGCCCTATGCATATAGCATTGCTACAAAATGGCAGATGCACTTTAACAATCGTGTTTAA
TAAAAGGTTGGATTTGCATATCTGAAGTGGGGCATGCAGTCTCCAACTGAACACAAGCCTC
ACTGCTCCCGCATGTGCACTGCACCTTCATATACATATTTCCTGCTTGGCTCCTGAGGGAATT
TGAGTAATCCCAAGAGGAACCCCTGTAGAAAATGTCCCCTGGCCACACACCCCCATTCCTAA
GGATGCAAGCAGGAGATAGAAACATTCCCTGCACCTCCCTCCTTGTTGTCAGAAGAAGTGC
AAAGAGTTGAATCCTTCCTAATGCCCACTTCTCACCCACGCCCCAAATCCCCAGGTCCCATG
GAGGTCCTTGGGGGCCTCCTATATCCTGGTGGTGTCAGGTTGATTTGGAAATGTCAGTGTCC
TCCCTTGTCCTCTCTGGCAGACCCTGGGTATGTGTATGTTTCAATGGAAGTGAATTTAAATGT
ACTTTATAAATCAAAGACTTTTTCTGAGACTTTGGAGAGTTCCAGTAATGAGAGCTTCTCATT
GTTATCAAGGCCAGGGCTGGAGACCAGTGGCAGGTGAGTTCCTATTGCTGTGATTGTCATGA
TGATGTTGATGAACAGTCACTATTTATTGAGCGTTCTCCATGTGCCAGTCACTGTACTAAACA
TTATTTCCTTTGGATTTCCCAGAAACCTCTCAGGTGGGTCTAATTACCCTTATTCAGCTGATA
AGGAAAGTAAGCAACTTACAAGACCACAGGGCTATGAAGTGGAAACACATAAATTGATATT
TCATTTTATTTATTTATTTATTTTGAGACAGAGTCTCACTGTGTCGCCCAGGCTGGAGTGCAG
TGGTGCGGTCTCAGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCGATTCTCCTGCCTGCC
TCCCGAGTAGCTGGGATTACAGGTGCCCACCACCACATCCAGCTAATTTTTTTGTAATTTTAG
TAGAGACGGGGTTTCACCATGTTGGCCAGGCTAGTCTCGAACTGCTGACTTCATGATCTGCC
CACCTCATCCTCCTAAATTGGTATCTTTATATGTCCAAAAGAGTCAACTGGTGGCAATTTAGT
GAGGTTTAATCTAATAGGAAATGATAGAGCTGGGATCGAACAGAGCCATGTGAACTCAAAA
CCTATGCTTCCCCTTCCACCTTTTTGAAAAACATTGTCTAGGCTGGGCACGATGGCTCATGCC
TGTAATCCCAGCACTTTGGGAGACGGAGGTGGGTGGATTACATGAGGTCAGGAGTTCGAGA
CCAGCTTGGCCAAAAATTAGCCAGGCGTGGTGGCGCGCGCCTGTGGTTCCCACTGAAGCAC
AGGAGGCTGAAGCACAAGAATCACTTGAACCCGGGAGGTGGAGGTTGCAGCGAGCCGAGA
TCGCACCACTGCACTCCAACCTGGGCAACAGAGAGACTCTGTCTCGAAAAAAAAAAATTGT
CTACATGCTGGTTGCAGAAAATTTAAACACTAAAACTAAAAAAGTAAAACACCTCCCAAAC
TTAGAGACAATATTAATGACGGAAAAAAAATTCTTCAAGATCTCTCTCTCTCCAGTCATTTAT
TCATGTGCGAAAACAGTTGGTGATTATTGATAAAATAGCTTTTAG
AGTTTGGAGCAATTATGTGCATTACATATACCATTTGATTCTGGCAACCTAATGAAGGAGTAT
GATCATTTCCCCTATTTAACAGACAAGAACAAGAAGAGGGAGGGCAGATGGTGTGGTAGTC
TAAGGCACAGGCTCCAGCAGATTATCTAGGTGTAAATCTTGGCTGTAGGCCAGGCCCTGTGG
CTCATGTCTGTAATCCCATCACTTTGGGAAACCGAGGTGGGCAGATCACTTGAGGTCAGGA
GTTCGAGACCAGCTTGGCCAACATAGCGAAACCCCTTCTCTATTAAAAATACAAAAATTAGC
CGGGCACGGTGGCAGGCACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCA
CTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCCAAGATCTTGCCACTGTACTCCAGCCTGG
GTGACGAGTGAAACTCTATCTCGATATTAAAAAAAAAAATCTTAGCTCTACCCACCGGGGCA
AGTTACGTAACGCCTCTGTGCCTTGGTTTTCATATCTGTAAAATGGTGACAGTAACAGCACC
CACGTCAAAGTGTGGTTGTGAGAACGAAACAAGATAGTCTATGTAAAGTGATTAAAACAGC
GTAGGCACATGGTAAACGCTTAGGAAATGTAGGCTGTTATAAAGCTCAGAGATGTTAAGTAA
CTAGATCAAGATCACACAGTTAGAGGGTGCCAGAGTCCTGATTTGAACCCAAGTTTGTCTCG
TTCTGGAGCTCAAGCTGCTAACCCTTTTTCAAAACTGGAATTAAACCAAAGTGCTCACCCTC
CGCTTTGCTGGGCCCCTCCCTGCCCTCAGGTGCGTCTCTTCCACTCACCTGCCACAGCAGCC
TCTGCTCAGGGTCTGAGACCGGGAAAGGTGAGGGCTACCCAGGTGGCCCTGATGTTTTCTG
CCAGCCAGCTCACCAGGTCCCTCGCAGCAGGCGGCAAAGGGAGGGAGGTTTGCTGTGAAG
ATTATGTGGTTCCCAACAACAAGAGCGCTGGGCCTATCTCTGCCCTCTCTTTTCTGTGTGTCC
TGGGACAAGTCACTTGGCTTCTGTGGCTTCATTTTCTCATGTGCCCAGCCAGGGGGTTGGCC
CTCATATGCAATAACAGCAGCAATGACCTTTACTGAGTGTCCATGTGCGTCAAGCACGTGTG
CTTTACACTTGTTCTTATTATTAGGTTTAATAATAGAATAATTGCCACATTTACTGAGCACTCAT
TATGGGCCAGGCCCTGCCCTAAGTGCTTAATTAGCTTTAGCTCCTCTAATCCTTATCTTATCCC
CACACGGCATGTTATGTTATCCCCATTATTCAGTTGAGAACATTGAGGCTCAAAGAGGCAAA
GTAACTTGACCAAATACTTGTAAACGATCTTGCATGCCCCTTCCAGCTGCCATTTAGTAAGAC
TCTAATTTCATACCACCCTAAATCTCGTCTGCTTCCCCCTCCTCCTTCTCGCCATCTCCCCACC
GAGCAG
The "TTGGCCAAGATCTGACCGTGATGGCGGCCATTGGCTTGGGCTTCCTCACCTCGAGTTTCCGGAGACACAGCTGGAGCAGTGTGGCCTTCAACCTCTTCATGCTGGCGCTTGGTGTGCAGTGGGCAATCCTGCTGGACGGCTTCCTGAGCCAGTTCCCTTCTGGGAAGGTGGTCATCACACTG TTCAG" sequence segment is: rhdexon2
GTATTGGGATGGTGGCTGGATCACTTCTGGGTCATAGAGGGAATGGACCCCGAAAGGACAG
GTTCCAGAAGATCTGGGATATTGCCCCCTCTCTGTCTAGCACCAGTGCTGTGCAATATTTAGG
ACATCCTTATACTAAAAGATTATTCATTGTTTAAAATTCAAATTAACTGGGCATCCTGTATTTT
ACTGGACAGCCCTACTCCGTGTATCACAAGGAATCCAGGCCTACATTCCTCCTGCATCCTTT
CTTTCCTGTTATTGTCGATTATGATTTTGTAAAGTTACATAATCAATATAAGTTTATGGAAAAC
GTAAGAAGGAAACACGTTAGACAGAGAGAAATAGACATGCCACACCTAGAGAGACATTCTA
TTTTTTTTTTTTTTTTTGAGACGGAGTTTCACTTTTGTTGCCCAGGCTGGAGTGCAATGGCGC
TATCTCGGCACACCACAACCTCAGCCTTCTGGGTTCAAGCGATTCTCCTGCCTCAGCCGCCT
GAGTAGCTGGGATTACAGGCATGTGCCACCGCGCCTGGCTGATTTTGTATTTTTAGTAGAGAT
AGGGTTTCTCCGTGTTGGTCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCGCCCGCCT
CGGCCTCCCAAAGTGCTGGGATTACAGACATGAGCCACCGCGTCCAGCCTGAGAGACATTC
TCTTGAAAAGAAAGGACTTTCAGCCCCCTAATGCTGCTAGACAATAAATAGCCATGCCTTTA
TTTTCATTAAATTACCTGTGCTTTGTTTACATGCATTTGTGTGAAATGCTAAGAACCATCACA
ACTAATGTATGGTGCCAGAAGTCAGAATAGTTGTTACCTGGGCAGGAGGTGGATATTGATTA
GGAAGGAACACAAAATAACCGCATGGGGTGCAGAAAATGTTCTCTATGTTCACCTGGGTGA
TGATTACACATCAAGCTATACACGTTTTAAAAGGGCATTGGCACTTAATAGGAGGAAGTAGG
CTAAATTTTTTCCTGAAACATTGTTTTGTTTTGTTCAAACCTCTGAATCCCTGTGCTGCCCAG
ATGATGGTAAACGTCATCCTAGGCATCTTAGGGACCTCTCAAGGCCATTCCAGCCTCCCCTT
CTAAGACCCTGCTAAACCTCTGGGCACTGCTGTTAAACATTTCTCTATGAGCCAGGAACTGT
GCTGAGCACTCCACAAATATTATTTTGTTTAACTCTTCCGGGTAGGGATCTAACCTGGTATAC
AGGTAAGGAAGTGGAAGCTCAGAGAGGGCAAGGCACTTGCCTAGGGCCACACAGCTAAGT
GGTGGAGATGGCTCCAACTTTTTATTATAACCTTTTCCACATGCTCCAGAGTGCTCAGAACAT
GAAACACAGTCTAGCCAGCTCCCGATTGGCCCTGGAGGGAAAAAACTTTATATATTTTTCTT
TTTTAAAAGGTTTAGAGGCTGGGCATGGTGGTTCACACCTGTAATCCCAGTACTTTTGGGAA
CCGAGGTGGGCAGATCACTTGAGCCCAGAAGTTTAAGACCAGCCTGACTAACACAGTGAGA
TCCTGTCTCTGCAGAAAATAGAAAAATCAGCTAGGCGTGGTGGTGTGCACCCACAGTCCCA
GCTACTTGGGAGGCTGAGGCAGGAGGATCACCTGAACCCAGTGAGGTTGAGGCTGAGTGA
GCCATGATCGTGCCACTTCACTCCAGCCTGGACAACAGAGTGAGACCCTGTCTCAAAAAAC
AGTTTTAGGGGCCGGGCGCAGTGGTTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGG
CGGGGGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACTCGGAGAAACCCTGTCT
CTACTAAAAATACAAAAAATTAGCCGGGCGTGGTGGTGGGCGCCTGTAGTCCCAGCCACTC
GGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGTTTGCAGTGAACCGAGA
TGGTGCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAA
CAAAAACAGTTTTAGGCCAGGCGCGGTGGTTCATGCCTGTAATCCTAGTACTTTAGGAGGCC
TAGCAGGTGGATTACCTGAGGTCAGGAGTCCGAGACCAACCTGAGCAACATGGTGAAATCC
TGTCTCTACTAAAAACACAAAAATTAGCTGGGTGTGGCGGCAGGCACCTGTAATCCCAGCTA
CTTGGGAGGCTGAGGCAGGCGAATCACTTGAACCCGGGAGGCGGAGGCTATAGTGAGCCG
AGATCGCACCATTGCACTGTAGCCTGGGCGACAGAGTGAGGCTCTGTCTCAAAAACAAAAC
AAAACAAAAACAGTCTATGAGTTAATTCCCACCAGAATTCAATACACACACGCACACATGC
ACGCATACACACACTGTGTCCACCTGGGAAGTGACAAAGGGCACCCTGGGGGATTTCAAAT
GGTGGTGGCCCTGGTTTGGTGTTGCTGCCTTAGCTTAAGGTCACACCAGCCTTCAGCCTCCT
GCCCCACAGTCTAGGGCTGCTCCCCTCATCTGATGTCCACAGGGACCTGTTTGTTCTTGACT
CAATCTAGAAAGACGAGAAGGGAGAGAAGTCACTCGCAGCCTGAGTGAACTCCCCTGCCC
CACCCCTGACTGCTTGGATCCCCCTAGGGGTGACCCCTGCTGAAACTGGCTCCTTCCTGACC
GGTTCCCGTCAGGGCTGTGCTGATGGGTGGTGCCCAGGCCTGCCCCTGGGGACGGGGTACT
CTCCCTTGGCAACACTCCAGCTTGTGCCACTTGACTTGGGACTGATTTGGTTCTGTTTTGAG
TCCCTTCAGGGGAGGGGCCTATCTTATTCAACGTTGTTGTTTGTTTTCCTCACATACTGATAA
CTTAGCAAATGGCTATTGGAGCAAAAATGAAAATAAACGGAACTCTGAAGTGGGATGTTTTA
AAATTTTATTTATTTTTTTAGAGACAGGGTCTTGCTCTGTTGCCCAGTCTGGAGTGCAGTGGT
ACAATCATAGCTCATTGCTATAGCTTAAGGACTCACCTGGCAGCAACACCAAACCAGGGCCA
CCACCATTTGAAATCCCCCAGGGTGCCCTTTGTCACTTCCCAGTGGTACAATCATAGCTCATTGC "the position is RHCE recombination initiation site"
AGCCTCTGCCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCCTCCTGAGTTAAATTTTTTTAC
AGACGCCTGCTACCATGCCCGGCTAATTTTTGTGTTTTTAGTAGAGACGGGGTTTCACCAGG
TGGGTCAGGTTGGTCTCGAACTCCTGACCTCAAGTGATCCACCCGCCTAGGCCTCCCAAAG
TACTGGGATTACAGGCGTGAGCCACTGTGCCCGGCCTAAAACTGTGTTTGAGACAGGGTCT
CACTCTGTTGTCCAGGCTGGAGTGAAGTGGCATGTTCATGGCTCACTCAGCCTCAGCCTCAC
TGGGTTCAGGTGATCCTCCTGCCTCAGCCTCCTAAGTAGCTGGGACTATGGGTGCACACCAC
CACGCCTAGCTGATTTTTCTGTCTTCTGCAGAGACAGGACCTCACTGTGTTGCTCAGGCTGG
TCTCAAACTCCTGGGCTCAAGTGATCTGCCCACCTCGGCTCCGAAAAGTACTGGAATTACA
GCCTCCTGAGTAGCTGAGACCACAGGCACACACCACCACGCCTAGCTTTTTTTTTTTTTTTT
TTGCTTTTTGTAGAGATGGAGTCTCACTATGTTGCCCAGGCTGGTCTCAAACTCCAGGCCTT
AAGCAATCCTCCCACCTCAGCCTCCCAAAGTGCTAAGATTACAGGTGTGAGCCACCATTCCT
GGCCTTAAAAGTGTGATATTTTTAATGTATTTTGAAATCTGCAGGACTCTCCCTAGAAGATAA
TAGCAATAACCAACTCCTTTATTGTGCTTGACGTATATCAACTCACTTTGCCCTTACCGTGGC
TCCAGAGGCATTGGGTCCACCTTATAAATGGAGGCACCAAGGCACAGAGTGATTAAATAAG
TTGCCCAGGATCACACAGCCAGAAAGTGTCTGAGTCAAGATTCCAGCCCAGGCAGCCTAGA
CCTGAGAGCACGCTCCTAACCACTGCACATCACTGTCTTAGCACCTCCTCAGCACAAACTG
GCCCTTGAGGAATGAAATACCGCCGCCGGCACACACGCTCCTGAGTTAAGCCTTTGTCAAT
GAAATGAACACCCACTTAAAAGGAATAACCTGTCCAGGCACGATGGAACATTGAATAACCC
CTTATTCTAAATTCCTGGTCCCTGTAAGACTCCTTCCCCATGCCCTTGCCCTTTTATGACCTTC
CCCTAAAGTCCTTGAGGCTTAAGCGGGCATAGTCTGCAGCAAACACTGGGGAAGCTGAGTC
CAGACTTCAGAGCACAGGCTTTGGATCTAGGCCAGCTGGATTTGAACCTCACATTTGTGATC
AGCTGGCATGACTGTTTCCAAAAAGTCCATTTTAATCCTCTACGTGACCCTCTGTAAAATGG
GGATACTGAACGGTGAGCTAGCACGATTTTACAGAGAGTGAATTTTTTTTTTTTTTTTTTTTT
TGTGAGACAGAGTCTTACTCTGTCGCCCAGGCTGGAGTGCAGTGGTGCAATCTCGGCTGAC
TGCAACCTCTGCCTCCCGGGTTCAAGCGACTGCCATGCCTCAGCCTCGAGAGTGGCTGGGA
TTACAAGCATGCACCACCATGCCCGGGTAATTTTTGTATTTTTAGTTGAGACAGAGTTTCACC
ATGTTGGCCAGGCCACTCTTGAACCCCTGGCCTCAAGTGATCCACCTGCCTTGGCCTCCCAA
AGTGCTGGGAGTACAGGCATGAGCCACTGCGCCCAGCCTTATAGGGTTAAAATTTAAAAGA
GGTGATGCTGTTACAAGCCTGTTTTACAAAATGCTCTTATAATAAATCATTATCATCACTGTTG
CTGTGGTTGTAGCATCATCATCATTAACTCCCAGAGGGAGGAGGGAGTCTCAGAGCAAGCT
GCTCAGGGGAGACTGGATGTCCATGGATTGTCCAGCTCAGTACCACTTCCTCCAGGAAGTC
CTCCCTGATAAGTCCAGTCAGCATCACCCTCTCCTTCCAATGAACCCCACTAGCCTTGTGATA
TCACAGATATTCTTAGTTGACAGGCTCATGGTGTATGTAGCCTGTCTAGATCATAAGTACATTT
TTTTTTTTTTTGGATCATAAGAACCTTCAAGACCAAAATAATTTTCTCCTCCTGAGCATGCTC
ATTGGTCAAGGGAAGGAAGGAATCGTAATAGTGTTAATAAGGCTAGTGTCTTTTCAGGAGTT
GGTTCTTTGTGCCAGTCTTGGTGCTAGACACACCGATAGGAAGAATACTCCTTCACATCCCC
AGGACACCAACATGGGATACGTTTGATCATCATTCTTAATTTGCAGAAGGAGAAATAGGCTC
AGTGAGATGAAATAGCCACTCCAGTGGCAAGGCTGGGACTGGAAGCCGGGCTTGTCCTGAT
TCCAAATCCAGTTTCTTTCCACTGCCACGGAGAGGGAGAGAAGGGACAGTGGCCCCAGATG
AGGATGGGGTGACTGGATGTGGGCAGGCCTGCGGGGGAAGAGTGCCCTCTGTTGAGCATCC
GAATGATGGCAGCAGAAAAGAAGACTGGGCAGAATCCCAGTTATCAGATCCCCTGAGGGAA
CAGTCACCCCGATCACCCTCAGTCAGATGAGTGTGTGTAGATCAATGCCTCATAGATGAAGG
CACTGAGGCACAGAGTGGTTAAGTCATCTGCCAGACCACATGGCTCAGGGTGCAGAGGCCA
CCTTAACGGGAGAAGAGATGGTCACTCCACTCTGCAGCATCAGCGCCCAGGTGGGTAGAAA
TCTTGTCTTCTATTTCCACAGAAAGTAAGGTGCCCAACAGTGTTTGTTGAATGAATGAATGA
ATGAATGAATGAGTGAGAGGCATCCTTCCTTCTCAGTCATCCTGGCTCTCCTTCTCACCCCC
AG
The "TATTCGGCTGGCCACCATGAGTGCTATGTCGGTGCTGATCTCAGCGGGTGCTGTCTTGGGG AAGGTCAACTTGGCGCAGTTGGTGGTGATGGTGCTGGTGGAGGTGACAGCTTTAGGCACCC TGAGGATGGTCATCAGTAATATCTTCAA" sequence segment is: rhdexon3CGTGAGTCATGGTGCTGGGAGGAGGGACCTGGGAGAAAAGGGCCAAAAGCTCCATTTGGTGGGGCTTCCGGGGTTTTGAAAAATAAAGACAACCTGTAATCCCAGCTACTTGGGAGGTTGAGGAGGGAAGATCACTTGAGGCCAGGAGTTTGAGACCCGCCTGGGCATCATAGCAAGATCCTCATCTCTAAAAAGTAATTTTTTCTAAATTATCCAGTTGTGGTGGCATGCACCTGTAGTGTCAGTTACTCAGGAGGCTGAGGTGTGAGTTGGAAGGATTGCTTGAGCCCAGGAGTTAGAGATGAACCTGGGCAATATAGCAAGACCTCATCTCTAAATAAATAGGTAGGTGGATAGATAGATAGATAGATAGATAGATAGATAGATAGACAGACAGACAGACAGACAGACAGACAGACAGGCTGGGTACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGAGGGCAGATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGGTCAACATGGGGGAACCTCATCTCTACTAAAAATACAAAATTTAGCTGCGCATGGTGGCAGGTGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAAGAGAATCGCTTGAACCCGGAGGGTGGAGGTTGCAGTGAACTGAGATCGCGCCATTGCACTGCAGCCTGGGGGACAAGAGCAAGACTTCATCTCCAATAAAAAAAAAAGAAAAAAGAAAAGAAAAGATTGATAGATAGATAGATACCCAAATGAGGTTACAAAAGTGTGGTCTGTGCAAATGTTTAAACACAACAAACCAGTGCCTTTAACTACTACAGTATAATCCTGTAGGATTGTGCTATTCATGATGTAATTATGGTTGTATAAAAGTAATTAATTCTCAGAGCCTCACCAGCAGTGGGTCCAGCAAGTTTGTACAGCCAGCATCTTCTTTCAGTCAGTGCGTGTCAGTAACTGCACATGTCCTCTCATTGGGAGAGCCTGTCGAAAGTCTAAGTTTGAAGGCAGCTGTGAAGGTAAGGCCAATCCAAATGGCTCTCCCAGCTCCTCTGCTGTAACCCTGACCCTGAGTGAGGACATAGCCAACCTTCCCATCTCATAGGTGAGAAGGCTGATGCCTGGAGAGGGGAAGGGACTGCCCAAGATCACATAGCAAGATAGTGGCAGAACCCAAGCGAGAACCCACAGTTCCAGCCTGGCTTAGAAGAAAGTGCACTGGACTTGGAGTCAAAGGCTGGGGTGTGCATCCCAGCTCTGCCATAAATCCCTGTGTGACTCTGGGCAATTTAACCTCTTAGAGCTTTAGTTTCTTCGTCTGTAATATGAGGGTAGCAGTACTACCACATAGGGTTTTGAGGGAGTAATTGAATTAATCACATGAAATGATGCACGTTTACAAAAAAAAGCATGAAGCCCCTTTACTGTGCCTCAGTATCCCAAAGGACTTTGGATTTACTCTGAGAAATACAGGGAGAACTAGGGAGTGTTGGGCAGAGGAGAGCTATGATCTGACTTATGTTTTAAGATACTCTGGCTTCTGGGTTCAGAAAAGACTGAAGGGGCAAGAGAGGAAGCAGGTGGAGACCAGAGCAGCAGTGATGGCCATCATCCAGACTCAGACTAGGACAATAGCTGTGAGGGTGGTGGGAAGTGATTGGATCCTGACTATATTTTAATAGCAGAATTGACAGGATTTGCTGATAGACTGCACGTGGGGTGGGAGAGGGTCAAGATGACTTCAAGGTTCTCATCTGGCACAACTCAGCAGCTGCTGGTGCCATTTACTGAGATGGGGAACATTGGGGTGGGATAGATCTGGGAGGGAAAACCCAGAGTTCAGTGTCGAATGTGGTAGCGTTAGGGTTAAGGTTGGGGCGGGTAGAGATGTGTATGAAACATCCCAGTGGAGACACTGAATGGAGATGTACAAGTCTGAAGCTTAGTGGAAAGGTTAGGGCTAGGGATATAAATTTGGGAGTTGTTACAATACAGATGGTGTTTAAAGCCATGAGACCCAAGGAGATCACTCAGGAGTGAGGATAAAGAGAGATGGGAAGAAGTCTGAGGACTGAGTCCTAGAACACCCTGCATTTTAGAGGGGGGACATGTGTAAGAGCCAGCAAAGGAGACAGAATTGTGCTTGGAGAGGCAGGAGGAAGCCCAGGAGAGCGTGAGGTCCTGGAAGGCAAGGAAAGAGAGGGCCCCAGGTGGGCTGAATGCTGCTGAGAGGTCAAGTCGGATGAGGGCTGGGAAGTAGCCATTGGATTTGACAAGGAGACCTTGGCATGCATGGTTGTAGAGGAGGATGAAGGCAAAAGCCTGGCTTGACTGATTCAAGAGCAGGAGATGAGAAAGTGGAGACAGCATGCAGGGGCAGCCCTGCCAAGGACTTTGCTCTAAAGGGGAACAGAGAAATGGAGGAGAAGCAGGAGGGCAATAATCCGATAGAGAGGAAAAATCTGATGATACAGAAGAGAGATGAACTGCAAGAGTCAAGCCTTTGAGTTGGAAAGCAGGAGTGGGATTTTGAGCACTGATACCTTTAGGCCGATGCAGGGACAGTTCATCTTTTTTAAAATTATTATTATTATACAACATTTTATTTAAAAATTTATTTTCACAGAATACATTTTCACATTAGAGATTCCCATTGTGCGAAAATAACAATTTATTACTTATAGTTTTATATTTGTGGACAGATTGTTTTAGAACAAGTAGAATACATTTGAGAATTAAATCTCAGTTTACAATGGGTAATATTTTGATACGTCTATGGGGAAACTTGCCCTTAAATGGAACTTCTGTATCTTCAGAAGCACTCCAAGCGTTTCTTCCTAGGATTTAGAAATTTATAATATGAGATATCAGCATTTCCTAATTTTAAAATTTCCCTAGTATATGTAACCATCGGTAGGTGGTATCTACCGACTAGAGAGGGAAGTTTTTGAAAATTAAACACTGTCTAATTTTCTGCAAAGTTTTTATTCATGAATTAAGAGTATTTCCCTTAGTCCATTATTCCCAAGGCAAATATGGAAGTTTGATCATATGCTAATCATACTAAAGCTGGATTCTCTTTAAGAGATTGAGAAATTAAAAGGCAAAAGCTGATATATCATGTTTAGTTATACTGTGAGTCTTATAAGAAGCTGGGAGGCAACCCCATTAACTCACCAGAATACAGAACTCAGTCTCACAACTTAAATATAATTCCTCTCAAACCTTTTCCTCAAAGTTAAATTCTGAAAATAATCTTGTGATTAAGAGAAGAAGGCTGTCCACCAATGGACTTATCTGTTATTTCTTCCTTATTGTGAGCTTAATGGCATGACAAAGCAGAGGCAAAGAGGCATACATCAATTCTTCAAAGTAGGAAGTCAAAAAGGTCAGAGCTTCCACAGCATGGCAACAGCTTTGCAGATGCCCACATCGTGATAGTTGAAATAGCAAAGCCCAGCAAAGGTTAAAGCTGAAAATGCCAAAAGCCCTGCCTTGGCAGCTTTCTGCGAGGCATCCCCATGAACATAGTCAGTAACAACTTGTCCAAGGCCCCAGTGACCATGAAGAGTGAGGGCTGCAGCCAGGGAATAGTCCGTCGCAGAGCAAGGATTCAAATAAGCAGCCGGAAGCAGACCCGGGAGCAAAACACTGACAACCCTCTCGCTAGTCCAGTGGAGAGATGCAGCCTTGGAGCCAGAATGGTGGCTCGGTGACAAGTGTATGTGCTGCACTCCACACCATTCTGGGATAGGTCGGTCCTGAAGAAATGCTGAGATATGAGCAGGTCTGACCACTGGAGTTCGCAGCAACAGAGCTCGGCCTCCTTGGGCACCGCAAACGGCACTCAGCCTCCAGAGAACCGCCATCTCGTTCCTGAGGCGGAGAGTTCATCTTAACGAGAGAAATGGCAGGGACTGTGAATAGGCCGGCAGATTTGGTGGCGGGTGCCACAGGTTCAGTCTCCTGCAGGGAGAGGAGAAAATGCCTTACTAATTCCTTGTATTTTCTCAGAGAAACAAGAGGCACCGTCATCAGCCTCATGTGAGGGTGGGAAGGAGGGATGGGGTTTGCGGAGAGGGAAAGTGTGGTATGGTCATCTGTGGGAGTGGAAGAGAGTGAGAGGGCTGCAGGGGTGCAGCGGGACTGCAGGCTGGCACCAGGGTCCCTAGGGCTTGTAGTTGGTGGAAAGTGCATCAGTGACCAGGGCTGTGTGCAGCTGCTCCAGGCAGGTGTGGAAGAAGCAGAGTTGAACTTGCCCAGCCTGGAGTGCTGCCCAGAGTGAGCCCAAAGCCCAAGGGAGACCAGAGATGGGGCTGTTTGCAAAGGAGGAAGTATAACAGTAGCCCACAAAATCTGAGCTGGTTAAGAAAGGAGAGAGAGTGAAAATGGGGAGCCCAGCCTGGCAGCCTGGGTACACATCTCAGCTCAACCCACACTAGCTGAATCCATTTGGGCCCCTTCGTTGACCTCTCTGTGCCTCAGTTTCCCTATCTATAGAATGGGGATAAGAATAAGGCTACTTCCTAGGGCTGTTGTGAGGATTGAACAAGTGACCGAACACTTGTTCAATTTTGAATACTGTTCTAAAGCATTTAGGACAGTGCCTGGCATGGGGTAAGTGTTGCGGCAGTGCTGTTATTTTCATCATCACCATTGTTCTCAGGCTGCGTTGATTGGAGCTGCTGAAGGGAGGCAATTTAAGGAAGTGAGCCGGACAGATAGGAGGTGGTGGTGGTTATCAGGTGCGATGCTTGAAACTGAGGCTTCGGAGGCAACAGTTACTGGTAATGACAAGGTCTAAGGCTTGACAGTGGGTGGCAGAAGTGTAACGCAGGGAAAGAGACGAGCGGTCAAGGAGC "partial position with intermediate deletion of this position as RHCE exchange"
GTTTTGATAACCTGAACTGTGTTTGGAGCTGGGGAGGAAGCAAACTATTGAATATATACAAA
GATGGCAAAGATGAGGGCCTGGAGCTTGCCACACGGAAGGGGGGATGGCTGCCTGAATGG
TTGGGCAGGTAGTTGTTGACATCTGCACTCCCTACAAGAGCAGCAGGGTGGCAACTCTTTTT
ATCTTTTTAATTTATTTTTCTTTTCTCTTTTTTTTTTGAGATGGAGTCTTGCTCTGTTGCCCAGG
CTGGAGTGCAGTGGCGTGATCTCAGCTCACTGCAAACTCCACCTCCTGGGTTCACACCGTT
CTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGCACCTGCCACCACTCCCGGCTAAT
GTTTTGTATTTTTAGTAGAGAAGGGGTTTCACTGTGTTAGCCAGGATGGTCTCCATCTCCTGA
CCTCATGATCCACCCGCCTCGGCCTCCCAAAGTGCGGGGATTACAGGTGTGAGCCACCACA
CCCGGCCTTAATTTATTTTTCTAGTCTGCAGGTAATTCTTTTTAATTCTCTCCACTCTCCTATGA
TCTTATGAGGTAGGGACTGTGATTATTTCTCCCACTTTATAATGAACAATCAGTAAAGACAGG
GAAGATAACCAAATGACATACAAGGTGGGGTCCACCCCATGAGGCTGCAGGCTTGGAGCTT
TCCTTTGTCTTAAAAATGAGAACATGAGCTGCCCACCTGTTGAGACAAGAAATAGGAAAGG
CTTAAAAAACTGGCTTGTTGTGTACAACTATCCGTGGGGCTGCAGTGAACGGGCTGGCAGT
GCCCAGGTGCATGCTGAACCCTGGGACAATCACATTCAGCATCCAGGGGCCCCCGTAATAG
CTTAATGTTTGAATTGAACCCCTGGGGTTGCCTTGAAGGAGAGAGATCCTGGAAGTATGTTC
AAGGGGTAGGGATGGGCAGGGGAGATGGGTCTGAAAGCCAAGCTCTACCCCACCCACCTT
GCCCCAAGAGAAATAGAACCTTCATCTTTAATTGCCTAACGAGAAAACTGGGGCTGGCCAG
ATGTGGTGGCTCATGTCTGTAATCCCAGCAATTTGGGAGGCCAAGGCGGGCAGATCACTTGA
GGTCAGGAGTTCGAGTTCAGCCTGGTCAACATGGTGAAACCCCGTCTCTATTAATAATACAA
AAATTATCCAGGTATGGTGGCGCATGCCTGTAGTCCCAGCTACTTGAGGCACAAGAATCGCT
TGAACCTGGGGGACAGAGGTTGCAGTGAGCCGACCACTGCACTCCAGTCTGGACGACAGA
GTGAGACTCCATCTCACAAACAAAAACAGAAAAAAAAAAAAAAAAAAGAGAGAGAGAGA
AAACTGGAGGCTCTGAGAGGTTAAAGGACTTGCCCAGGGTCTTGCAGCTAGTAAGTGACAG
AGCTGGGACTTGAGCTTGGGTTTTCTGACTCCTGGTCTGGTTCATTATCCATGAGGTGCTGG
GAACTAAAATAAGCCACAATCTTGGAATCTCCGTCGCCTCCCTCCCTCCCACATGTCTGCGT
GGCTTTTTGGGAAAATGCCAGGGGAATGTACCAGCCAGGGAGAGGACCCTTGTTTTCCTCA
TGGCCCTTCCTGGCAATGGCACTACTGACACCGACAGTCCTTTTTGTCCCTGATGACCTCTG
CTGCCTGATGCCCAAGTGACCACCTCTGCTTTGTCATTTCTAG
The "GATTGGCTTCCAGGTCCTCCTCAGCATTGGGGAACTCAGCTTGGCCATCGTGATAGCTCTC ACGTCTGGTCTCCTGACAG" sequence segment is: rhdexon8
GTCAGTGTGAGGCCACCTTTCTTCCACCATTGCCAGGACACAGCACCCACGTCCAGAGCGC
ACCCTGCCGTGTGGCTGGATGTCTATGTGCCCCATCTCCTTCCCTGAGGATCACATAATTTCA
GAATTGGAAAGGTTCTTAGAGGTCACCTGCTGCTAATGTGGACTGTGAGGCCAGGGCAGGG
AAGGGACATCCCTGAGGTTATAAGTAGGGTGAGTGGCAACGTTGCAGACTTTTGAACCCAG
GGCTGGTGATCACACTCAGTTTTGCACAGAAGCCCGAGAAAATCCTTACACCCAAAAGCCT
ACCTTTTATTTCTGAGGACACCCATAATACTATTTTATTCAACAGATATTTATTCAATATCCACT
ATGAGCCAGGCACTGGGGACACAGCAGTGAGCAAAACAAATTCCCTGACCCCATGGAATTG
ACCTTCTAGTGGGGGAAGGTATTAGCAATAAATAGACAAATAAGTGTCTACTACGCCAGATG
GGAAGAAGTGGCTGTGAAGACAGAGCAAACTAGAGAAACATAGAGTCAATGTGGGATGGG
GTGTTCTTTTAGGGGGGTGGTCAGGGAAAGCTTATCTGAGTAGTTAGCTTTTAAGCAGAGAC
CCCAATGAAGAGGAGGGAGATATGCGATGCATTTAGTTAGGGGAAGAACATTCCATGAAAAT
AGGATAGCAAGTGCAAAGGCCCTGAGACAGCAGCATGCTTTGTGTGTTGAGGGAACAGTA
AGGAGACCAGTGTGGTTGGTGTGAATGGAGTGAGAAGGAGCAGCAGGGGTTGAGGGCAG
AATGGTAGTGAGGAGCAGGCCCTTATAAAAGATGGGAAGCCACTGGAGATCTTTCAACAAA
GGGGAAAAGTATGTTTCTGTTCTTGCAATACAATAGAAAAGCAAAAAATCTAGGGGAGTTG
CTAATTAGCCAGTTTTACTTATATGCCAGGTGAAAATATGTGGCTAGGTGCAGTGGCTCATAC
CTGTAATTGCAGCAGTTTGGGAGACCGAAGTGGGCAGATCATCTGAGGTCAGGATTCAAGA
CCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATTAAAAAATTAGCCAGGCGT
GGTGGTGGGCACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATTGCTTAAACC
CGGGAGGCAGAGGTTGCAGTGGGCCGAGACTCTGTCTAAAAAAAAAAGAAAATACACATT
CAGGCCAGGCACAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTAG
ATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGGAAAACCCTGTCTCTGCC
AGAAATACAAAAATTAGCCAGGCGTGGTGGTGTGTGCCTGTAGTCCCAGCTACTCGGGAGG
CTGAAGTAGGGGAATGGCTTGACCCCAGGAGGTGGAGGTTATAGTGAGCCAAGGTTGCACC
AGCCTAGGTGACAGAGTGAGACTGTCTCAAAAAAAAAAAAAAGAAAGAAAATATACATTC
CATCCAGAACTTGTTATTCTACAAGCAAACATCTTTTATTGGTTAGACACCCATATATGTGTCC
CTAAGCAGGAGGTGGATGCCAAATAAGAGACAAATGGCGTAAGACACTATGAGTTGTGTGG
TGACATTGGGCATGTCACTTCACTCCCTCTGAGCCTTGGTTAGCTTCTCTGTAAAATGAAAG
GATTATGGTAACTAAGCTGGCTTCCTTCCAGCTTTAACAAACTGTATGGAGGTACATTTTGGA
GTTACTTGGGTAATTTTTGAGTGTGAGATTGGCTAGAATTGCTTTAATATACCAATGTCTGGC
CTTAGCTTTTGGCAGAGTCTGTGTGAAGAAGCAGAGGCGGAGTAGAGTTAATTCCGTAAGT
TAACGTTCAGTTCGTGGCAGCTGGCAATCCAACCCTGGGAAAGGCTGCCGGATTTAGCAAA
AATGCAAGGTGTCTGTTTTTAAATTCGCAATGAATTGGGTATCCTGCATTTTATTTGGCAACC
CTGTCCTGGGACTCACACTATTCACTGTTATCACTGGTATATTCGAAGTGGTGCTGACTTGCC
CTCTGTCTTGCAAAGTACCCGGGGGTCTTTTCTTATGCTTCACTGGAGTCAAAAAAGAGAAT
AGAGGAAAAGACAATCATATTGTTCCTTTAAGAGTTAAGACCAACAAGCTTTCTTCTTTACA
TGTTGTTTTTGACATGAGCAAACTGGTGATTAAAAACAACTTGGGTGGCTCATACTTGTAAT
CCCAGCACTTTGGAAAGCTGAGGTGGGAGAATAGCTTGAGGCCAGGAGTTCAAGCCAGGG
CAATCCTATAGTGAGACCCCATCTCTACAAAAGATACAAAAATTAGCCAGGTGTGGTGGTAC
ACCTGTAGTCCCAGCTGCTCCGGAGGCTGAGATGGGAGGATCAGTTGAGCTTGGGAGGCAG
AAGTTGCAGTGAGCTGAGATCGTGCCACTGCACTCCAGCCTGGACAACAGAGCAAGACCC
TGTCTCAAAAAAGGAAACAAAACAACTTGGACAATGGAAGGGGGAGAAAGTTCCTCAAGA
AGCCAAAATTGCACCAAATGGACTCCCAGAAGCCAAGCATTTAACTTGTTAATTGAGCCCTC
TGTGGGCCTGTCTATACTTATTTAAGGAACAATCCTATCAAGCATAGTTATTGGGTTTCTCAG
CCCAGGTAGATTAGAAATAGCAGATTAGAGGTGGGCTAGGTTTCTAGAGGTAAAGTACACCA
GCAGAAGTTAGAAGTGAAAGCAAAGAGCCTAACAGAGGAAGAGAAATTCTTTTTTTTTTTT
TTAGACGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCTATCTCGGCTCAACG
CAACCTCCGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTA
CAGGCATGCACCACCACGCCCGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTTCTCCATGT
TGGTCATGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCCACCTTGGCCTCCCAAAGTG
CTGGGATTACAGGCATAAGCCACTGTGCCCGGCCAACAAATTCTTAAAACTGGACACAAGA
ACACAAAACGCTTGGGCTGCTGAGAGATTAGACCAACAACCCTCCACGGCTACAAACCTTT
TCCACGTTATATGGCACGTTATAAGTGGGTGTTCCTAGTGATGGTTCTGATTTTTTTTTTAAAA
AGTCTAAATATGTTTAATGTTGTCTCAGAAGACAAAATATATTTTAGACAGATATTCCTCAGT
GATGAGTAAGCCTCAGCTATCTGGAAAATTCATGCAGGCGCCAGAGATCATTACTGAGTAAT
TCAAGCTAATAACTGCGTCATGCTGGTTGTACCCTGCATGCCAATATCTGCTAAAAGCAGCA
CCACGAAAGGGAAATACGAATCTCACTAAGCACTCACCCATTCTTGTTAACGACACTGGAA
CTGATCATCCTTAATAATACACAGATAAATCTATCAGGAGCATTTCCTTGCTTCCTGTGAAAG
GAAGTACTCATTCCATGTGTCCTGTGAAATTCAGCCAGCTTCGGGAAGCTGGAGGAATACAT
ATGGCCAAGCTACCTGGGCAGAGAGTAGACAGGGAATGGAGGTTGGGCACAGTGGCTCAC
ACCTGTAATTGCAGCCCTTTAGAAGGCAAAGGCGGGCAGATCACTTGAGCTCAGGTGTTCA
AGACCAGCCTGGGCAACATGGCTAAACCCCGTCTCTGCAAAAAATACAAAAAAATGAGCTG
GGTATGGTAGCACACACTTGTGGTCCCAGCTACTTGGGAGGCTGAGGTGGGGGGGTTGCTT
GTGCCTGGGAGTTTGAGGCTGCAATGAGCTGTGATTGTGCCACTGCACTCCAGCCTGGATA
ACAGAATGAGACCCTGTTCCAAAAATAAAAAATAAAATCAAAGACACTTAAAAAGATGGGG
AAAAGGAAGGACAGGCACTTAAGCAAGTTATAAGCTACTTTCCTAACTACACAAGTGGAAT
CTTAAGCTGAGGTTCCCAGGAGTTGACTGGAGCCAGAGAAGACAGACCTATAGGAGCACCC
AACTGGAGTCGCCCTCCATAGTAGCCCATATGTCTTACATGGATCAGCTTTCGTGGGGCCCTT
CTACTCCGTCTGGGGAAGGGCGTCAGATCTGTGGCTCTCATGTACTGCTCAGTACACTGCCA
TTCCCAGTTCTTTTTTTCAAAAAAAAAAAAATTGTTTACAGAATCGGCCGGGTGTGGTGGCT
TATGCCTATAATACTAGCAATTTGGAAGGCTGAGGTGGGTGGATCACCTGAGGTCAGGAGTT
CGAGACCAGCCTGGCCAACATGGTGAAACCCCATCCTACTAAAAAAAAAAAAAAAAAAAA
ATTAGCTGGATGTGGTGGCAGGCGCCTATAATCTTAGCTACTTGGGAGGCTGAGGCAGGAGA
ATCGCTTGAACCTGGGAGGCAGAGGCTGCAGTGAGCCGAGATCATGCCACGGTACTCCAGC
CTGGGTGATAGAGTGAGACTCTGTCTCAAAATAAATAAAATAAAATAAAATAAAATAAAATA
AAATAAAATAGTCTACAGAATTAAGCTGGTCCAGGAATGACAGGGCGTCCATTTATTTGTCT
TTCAATTGTGGGAGAAAAAGGATTTCTGTTGAGACACTGTCGTTTTGACACACACAATATTT
TGATTAATCTTGAGATTAAAAATCCTGTGCTCCAAATCTTTTAACATTAAATTATGCATTTAAA
CAG
The "GTTTGCTCCTAAATCTCAAAATATGGAAAGCACCTCATGTGGCTAAATATTTTGATGACCA AGTTTTCTGGAAG" sequence segment is: rhdexon9
GTAAGATTTTTCACCTATTAACGTGATAGATTTTGAGTGCATGAACTTAAAAACATACCTGGG
TATATATGTTGACTTGCTGTTTATGAGTAAAACAAAAACAAAAATGGAGTAAGGAGCATTGC
AGGAGGAACTAGAGGAGAAACAAATCCATGATATGCATGTGTGTGGGGGAGGGTGGCGGG
GAGGTGGTAAAGGTCACCATTTCCCTGATACCTCAAATTCATTCAGAGTCAGGGATGAGACA
GCTTTCACTGGCCACACTTCCCCTCCCGCTATCTGCAGTCCTCAGCGTAGCCAAATAGTTTG
ACATGCGGGTGACAGAACCCCGCAATGCAAAAGCTGGAAGAAACCTCAAGCCTTGGAGTC
CAACCCCTTTTTTGACAGATGCTAAGAGTGGAGACATGACTTATCAAGATCTTACAACTGGC
TGGGCACGGTGGCTGACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGGCGATCAC
CTGAGGCCAGGAGTTCGAGACCAGCCTGGCCAACGTGTCAAAACCCCATCTCTACTAAAAA
TACAAAAGTTAGCTGGGCGTGGTAGCACATGCCTGTAATCCCAGTTACTCAGGAGGCTGAG
GCAAGAGAATCGCTTGAAATCAGGAGGCAGAGGTTGCAGTGAGCTGAGATTGCGCCACTG
CACTCCAGCCTGGGTGACAAGAGCTGACACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAA
ATTCTTACAGTGTGTGAGTATCCAGGCTGAGTCCTGAACACAGCTCTTGATAAATGATAACA
AGCAGGCACAAAAAAATTGTAGTACAGGAGTCTGAGGTCACTTAGCAAAGGGACATAAAG
TTCAAACAGCTCAGCAGCTGCTGAGGGTCCCGTGTTACATTGTAGCATTTGTTGTTGTGACT
GGGCTAGAAAGAAGGTGAAGAAGGTTGGAGCTCACTCCCTGCCTCCCCTCCCACTCTCCTC
CCTTTGACCTACACTCATAGTTCACGCAGCACTCTGATGTGTCCCCTTAGGCCATCCTCTAGT
CAATGCTGTGGGTAGGCTGGACCAGCAGGGACCAGTATTGTCACAGCAAGTCCAGGCCAAC
AGTGGTCAGGCTGCTGCCCGGTGTTGTGCCTTTGTGAGTGGCAGATCCAAGACCGGAACCC
AGGCCTTCTGAGTCCCAGGCCAATGCTTGCCCCACCCAGCATCCAAGATGTTGCTCACTAAA
GAGACAGAGAAGCAGCCTTATTATGGGCCTGGACACCTGTGCATGAGGGGTCAAGCAGAG
AGGACCTGGGGAGAGACCCTGCCCCTTCTTTTCCTTCTCCTTCCTCTCCTTTCTCTTCTTCTT
CCTCTTCAAATAGCTTTTTGAGGTGTAACTGGCATACAATCAATTGTACATATTTAGGCTGGG
TATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACTTGA
GGTCAGGAGTTTGAGACCAGCCTGGGCAACCCGGTGAAACCCCGTCTCCACTAAAACTACA
AAAATTAGCCAGGCGTGGTGGCAGCTGCCTGTAATCCCGGCTACTCGGGAGGCTGAGGCAG
GAGAATCACTTGAACCTGGGAAGCGAAGTTTGCAGTGATCTGAGATCATGCCACTGCACTC
CAGCCTGGGTGACAGAGCGAGACTTTGCCTCAAAAACAAAACAAAACAATTGTACATATTT
AAAGTGTTGTAACCAAGTGAGTTACAGAGAAACACCACACTTTGAGCCTAATTCAGGAGTC
CTTTATTAGCCGGCGACCTAGAGACGACTAGTGCTCAAAATTCTCTCGGCCCCAAAGAAGG
GGCTAGATTTTCTTTTATACCTTGGTTTAGAAAGGGGAGGGGGAATTGAGCTGAAGCAATCT
TACAGAAGTAAAACAGGCAAAAAAGTTAAAAAGACAAATGGTTACAGGAAAACAAACAGT
TCCAGGTGCAGGAGCTTTAAAGCCATCACAAGGTGACAGGTGCGGGGGCTCTGGGTGCTAT
CTGCCGGACACAAACGCAGGGGCACTAGAGTACTATCACCCGGGCAAATTCCTGGGAACTG
CGGACACAGCTTGCCACAGTACCTTATCAGCTAATTGCACTCTTTGATGTGCTGGGAGTCAG
CTTGCACAAGTTAAGTCCTTGAGGAAGGGGGTGGGTAAGGAGCCCTTAACGTCTTGCAAAT
GAAGGAGCCGAATGGAATCCCTCCGGCTTTCTTAGCTAAGAGAGAGTCAATCAAGTTAATAC
AAGTTAGGGTATCACAAAAGTATATAATTTGATACATTTTAACGTATTTATACACTGAAGAGA
CCATCACCACCATCAAGACAAGGAGCACACCCATCACTTCCACACACTTCCTCCTGCTCCTT
TGAAATTCCTCCCTTCCTACCCACCTGGTCCCACCCAAAGGCAACCACTGAACTACTTTCTG
TCACTAAGGTTTGCGTTTTCTGTAATTTTTTTGTTTGAGACAGGGTCTCACTCCGCCACCCACACCGTAATGCAGTGGCACCATCATGA "the position is RHCE recombination termination site"
CTCACTGTAGCCTCAACCTCCCCAGGCTCAGGAGATCCTCCCCCCTCAGCCTCCTGAGTAGC
TAGGACCACAGGTGTAGGCCACCATGGCAGGCTAATTTTTGTATTTTTTTGTAGAGATGGGG
TTTCACCGTATTACCTAGGCTGGTCTCGAACTCATGGGTTCAAGCAATCCTCCTGCCTTGGCC
TCTCAAAGTGCTGGGATTATAGGCATGAGCCACTGTGCCCAGCCCTCTGTAATGTTACACAA
AGGGAATCATGCAGCACGTACTGCCCTTGGTCTGGCTTCTTTTGCTCAGCATGATTATTCTGA
GAATCATCCGTGTTGTTGCGTGTAACTGACTTCATCAGCTTCTCTCTGCAGCTGTCAGCTCTT
GGCTTCTCCCAACAGCCAATCTCTCTTTATCCCCTGCAAGTGTTCTTGCCTATTTAGCAGAAT
CAAGGTACTCTATCGAAAAGACTCGGAAAATTGGTTTAATCTATTCATTCATTCCTCAGGTAT
TTATCGAATAACTATTCTATACCAAGTACTATGCTAATCAACCAAGGACAGCACAAACAGGA
GAAATCTCCAGCTCAGTCACTTGAGTTGCAATAAATATTTGCTGGATACGTCAGGTGCAGTG
GCTCACACTTGTAATCCCAGCACTTTGGGGATTACTGAGACGGGAGGATCTCTTGAGCCCAG
GAGGCCAAGGCTGCAGAGAACCATGATCATGCCACTGCACTCCAGCCTGGGTGACAGAGTG
AGATCCTGTCTCTGAAAAAAAATATTTGCTGGATAAATTAAGGAAATCTGACGAACCCCATC
AGTAGCCATTGCAGCAACAGGTAAACTAGAACGAGTGTGAATTTGGAATGAGGAAACCCGA
TGTTGGCCATCATTCTGTAATGTCATGTATTATGTAATGTATTATATATTAATGTATGTATTATGT
AGGCAAGTTCCTTGACCTCTCTCACTGGTAACATAAGAGTAGTAATCTTTGTGCTACTTCACT
GGGTTATTTTAAAGATCAAGTGAGGTAATAATGTCTGTAACAACATTCTGTAAAATGCAAAC
CGCCACATGAATGTGAAAGTTTATTACTAGGGATTTAGCCAACCACAAGGGAATGTGTGAGC
ATAAGAGCTATCATATTGCAAGCCTACAGTTTCTGATTTTGTGCTAGGTGCTTTTCCACATTAC
CTGATTTTATCCTCACAACAGTCCTGCATAAAAGTAAGTATGTCGCCCAGGTGCGGTGGCTC
ATGCCTATAATCCCAGCACTTTGGGAGCCCGAGGTGGGCAAATCACTTGAGATCAGGAGTTT
GAAACCAGCCTGGTCAACGTGGTGCAACCCTGTCTCTACTAAAAATACAAAAAAAAATTAG
ACAGGCGTGGTGGTGGATGCCTGTAATCCCAGCTACTTGGGAAGCTGAGGCAGGAGAATGG
CTTGAGCCCGGGAGATGGAGATTGCAGTGAGATGAGATTGCGCCACTGCACTCCAGCCTGG
GTGACAGAGCAAGGCTATGTCTCAAAAGAGAAAAAAAAAGTAAGTATCTCAGTCTTGAAGA
TGATGAAATGGAGGCCTAGAGAGATTAAGTAACTTGCCCAAAATGACAGAACTAATGCATAG
AAAAGAAGAAATGTGATGTCTTTTGGCTCCAAAGACACCCCACATATGCGTTGGTTACAGTT
ACTAGAGAAAAGTTATTCCACCCCCACCCCACCCCCAGAAATCTTCTGACTTGTTTTCTCGC
AGTTGAGTAGGACCATTTATTCGGCAGTGTACCATTCTCAGCTTGCAGTTGAAAGCCAAATA
TCCATTAAAGAGGCAAGGATGCAAACTTGCTAAGCTGATAAATCCAGGGGTGATTTTTTTTT
TTTTTTGCAAACCATCCAACAAGACATTTTAAATACTCATTGAATTTCATAGAACTGACTGCC
AGGATTGGAAAGACATTAAAGCCAGCTCAGCCACTGCCTCGCTGGTTGGCCAGACCACGCC
TGGCACTTCTGGGAGGGAGCACTCACCACCCCCCAAGGGCACCCATCTCATCCTCCGAAGG
TTTATGAAAATGCACTCATCATTTGCTAATTCATTCCACTACGTGTATTACCTAATTTGTGACA
CGATGTGAAGTACCAGAGAGATAATTCTAAATAAAATATAGTTATGGGTCTCAAGGAGCCAG
ATATGCTAATCTCCTATCCTCCTGCAGTTTACAGTGGTCCTCACCAGATACTTATTTACAAAAA
TTCAGTTTATTATTTATTTTTTTGAGACAGAGTCTTGCTCTATAGCTCAGGCTAGAGTGTAATG
GTGTGATCTCGGCTCACTTCAACCTCTGCCTCCCAGGTTCAAGTGATTCTCCTGCCTCAACC
TCCCAAGTAGCTGGGACTACAGGCACCTGCCACCACGGCTAATTTTTGGAGTTTTAGTAGAG
ACAGGGTTTCACCACGTTGGCCAGGCTGGCCTCGAACTCCTGACCTCAGGTGATCTGCCCA
CATCAGCCTCCCAAAATGTTGGGATTACAGGCGTGAGCCACCATGCCCGGCCAAAACTTCA
GTTTATAACACAATCTTTCACGTGTCTTCTGCTTTCATTAAAAGAATAGACAGTTCCCTTCTTT
ATTTCAGTTTAATAAACCATGGATTTTATTTCATGCTTTGCAAAACACAAGGGCTCACTGACA
TGCACTTCTTAAACTAATTCTGGCTGGTCGCCTGTAATTCCAGCACTTTGGGAGGCTGAGGC
CGACAGATCACTTCAAGTCAGGAGTTCAAGACCAGCCTGGCCAATATGGTGAAACCACGTC
TCTACCAAAAATATAAAAAATTAGCCAGGTGTGGTGGTGCGTGACTATAATCCCAGCTACTC
AGGGGCCTGAGGCAGAAAAATCACTTGAACCCGGGAGGCGGAGGTTACAGTGAGCTGAGA
TCGCGCCACTGCACTCCAGCCTGGGCGACAGAGTGAGACTCTGTCTCAAAAAATAAATAAA
TACAAATAATGTAAAATACGAAACAAGCAATCCTGGCAGTAGCTGCTGGAATGAGAGGAGG
GAGAGGTCATAGGGAGGTCGGGGACAATGGAGCATGGAGTTGTGTTGGATTTGGCTAAGCA
GCAGGAAGTGCAAGGCATTCCAAGCAAGAGGAGGGGGGCAGGTGGGGAGCATCTGCAAG
AACAGAAGCAGCATGAGCAACCTGGCTCGGCAGTGTGTGAAAAGGCTGAAAGGTGGCTAG
AGCCACTTCAATTTCATCCTTCAGGCAAATGGGAAATTCCCAAAGGTTTGAGTGGGGAAGC
AATGCCTACAATGAAAGTTTGAGAGTGAAGCAGAGTGATCGAATTAAGCATGTAGGCCGAG
TTCTGAAATAACTGCAATGTGCTGAAGATCATCCATTGGCTTCTGAATGAGTATTTGCAGTTT
ATTTTTTAAAATGATTTTATTGCCAAGAAAGATAAACACTACTGTTTTGGTACAAAAACATAA
CAAAATGTGTTGAGTCCCTCTTGCTGTTTTACGCAAAGTTTTAAAAATCTACTCTTGTCACA
GTGGTATCACCCCTACTTCTGATTTCAAATAAATGTTCTAGAGACACAGTAAGGGCCCAACA
AACGCTTGTTCAACAACACAAGGAGAGCCAGCTTTTAAAGTAGGAAAACAGGCCGGGCGC
CGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGCTGAGGTGGGCAGATCACTTGAGGT
CAGGAGTTCAAGAACAGCTTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAACACAAAC
ATTAGCCAGGCGTGGTGGTGCACACCAGTAGTCCCAGCTATTCAGGAGGCTGAGGCAGGAA
AATGGCTTGAACTGGGGAGGCAGTGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAG
CCTGGGGGACAGAGGGAGACTCCATCTCAAAATAAAACAAAACAAAACCAAATCATACAA
AAACATTAGCTGGGTGTGGTGGTGCATACCTGTAATCCCAGCTACTTGGGAAGCTGAGGCA
GAATTACTTGAACCCCTGGGGGGAGGTTGCAGTGAGCTGAGATCTTGCCACTACACTCCAG
CCTGGGCAACAGAGTGAGGAGACTCTGTCTCAAAAAATATATATATTAAAAAAAAGAAAAA
AAAAAGTAAACTAGGAAAACACATCAGCAGCCTGCCAACAGACTCCCCTAGCCTCGGTGA
GGGCCAGTGTTCTGGGAGGCAGATCTGAATTCTAGTCCTAGTTCACCCACTGGCAGGCTGG
TGCCCTTGGGCAGGTCGCTTCTCTGGGGCTCAGTTTCTTCCTCTATAAAATGAGATCAAATCC
CATGTTCTAAGAGTTTGTGCTCTGGAGTCAGACAGATCTGGGTTCTACCACTGCCAGCTCTG
TGATCTTGTAGCTTCAGTCTCGTCATCTGACATGGAGATAACAGTAACTGTCTCACTGTGTTG
TTAGGGTTTAAAGGAGATAATGTATGTGAAATGTTAGCAAACAAGTGTTAGCTACCCTGATTT
CCGGTTTCAGAGTTCTGTGGTCCCAGTTTATGCCACATGCAGTGACGTTGTATGGTAGGCTG
TGGTGTGGCACCACTTCAGAACTCAGCGCATGCACAGCTTGCAGAAGAGAAGGCCAGAGG
AGACCTAAGAAGGCTCTTCGAACACTTGAAAGACCGGCATGTAGGCCGGGCGCAGTGACT
CACGCCTGTAATCCCAGCAGTTTTGGAGGTCGAGGCGGGTGGATCACCTGAGTTTGGGAGT
TTGATACCAGCCTGACCAACAAGGTGAAACCCCGTCTCTACTAAAAAATACAAACATTAGCT
GGGCATGGTGGCGGGTGCCTGTAATCCCAGCTACTCCGGTGGTTGAGGCAGAATTGCTTGA
ACCCGGGAGGCAGAGGTTGCAGTGAGCTGAGATTGCATCACTGCACTCCAGCCTGAGACA
AGAGCGAAACTCCATCTCAAACAAAACAAACAACCAACCAAACAAAACCAAAAAAAAAA
CTGGCATGTAGAAGAAAAATACTTTTTCTCTACACTTCTCCAAAGAATTTAACTAGGCCCAG
GGGAGGTGCAGTATAAATTTCTAACAATCTCAACTGTCTGCCAAATGGAATGAGCTACTTCA
TATGGCAGTAGTGAGTCCTCTGTCTTTGGAGGCATTCAAATAAAAGCCAGATGGCCATTTATC
AACAATCCATGTAAAACGTTAGATGAAATAAAACCTATATATCCAAGATCTCTTCCAATTCAG
ATTTTATGAAAGAATTTCTAAGGTCTTTGTAATGAGACATTTAGGCTGTTTCAAGAGATCAAG
CCAAAATCAGTATGTGGGTTCATCTGCAATAAAAATGTTTGTTTTGCTTTTACAG
The "TTTCCTCATTTGGCTGTTGGATTTTAAGCAAAAGCATCCAAGAAAAACAAGGCCTGTTCA AAAACAAGACAACTTCCTCTCACTGTTGCCTGCATTTGTACGTGAGAAACGCTCATGACAGCAAAGTCTCCAATGTTCGCGCAGGCACTGGAGTCAGAGAAAATGGAGTTGAATCCTTTCTCTGCCACTCTTTGAGGAGAATCTCACCATTTATTATGCACTGTAGAATACAACAATAAAATACAGCCATGTACCACATAACAACATCTTGGTAAACAACAGACTG" sequence segment is: rhdexon10
Other embodiments of the disclosure will be apparent to those skilled in the art from consideration of the specification and practice of the disclosure disclosed herein. This application is intended to cover any adaptations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims.
It is to be understood that the present disclosure is not limited to the precise arrangements and instrumentalities shown in the drawings, and that various modifications and changes may be effected without departing from the scope thereof. The scope of the present disclosure is limited only by the appended claims.

Claims (13)

1. An RHD gene primer, wherein the primer sequence comprises: primer 1: RHD1-F, nucleotide sequence:
CACAACCTGCGAAGCACGGGA；
primer 2: RHD1-R, nucleotide sequence:
CCTCGCCCACAGCCTCTACTCTACC；
primer 3: RHD2-F, nucleotide sequence:
GATGGTGTCAGGGGAGGGAGGTG；
primer 4: RHD2-R, nucleotide sequence:
AACAGAACCAAATCAGTCCCAAGTCAAGT；
primer 5: RHD3-F, nucleotide sequence:
ATTCTGGCAACCTAATGAAGGAGTATGA；
primer 6: RHD3-R, nucleotide sequence:
GCTCCTTGACCGCTCGTCTCTTT；
primer 7: RHD4-F, nucleotide sequence:
AACTCACCAGAATACAGAACTCAGTCTCACA；
primer 8: RHD4-R, nucleotide sequence:
CTTCAGCCAAAGCAGAGGAGGTTAG；
primer 9: RHD5-F, nucleotide sequence:
TCAGTGTAAGAACCATAAAGGTGTATCTGTGTAGT; primer 10: RHD5-R, nucleotide sequence:
TAGTTTTCTTATCAGTCGGTAGTTGTGTCA；
primer 11: RHD6-F, nucleotide sequence:
CAGGACTGTTGGGATGCTACTGGATTT；
primer 12: RHD6-R, nucleotide sequence:
CCAATAAAAGATGTTTGCTTGTAGAATAAAGGTGA；
primer 13: RHD7-F, nucleotide sequence:
GTTTTGATAACCTGAACTGTGTTTGGAGC；
primer 14: RHD7-R, nucleotide sequence:
GCAGTCTGTTGTTTACCAAGATGTTGTTATGT；
primer 15: RHD-BOX-F, nucleotide sequence is:
AAACAAATACCTGAAACACCTACACTACAAAAATG；
primer 16: RHD-BOX-R, nucleotide sequence is:
TGTAAGCCGAGCAGTAGAGACCTGAGAT。
2. the RHD gene primer of claim 1, wherein the RHD gene primer is synthesized based on 5' phosphorylation using primer 1-primer 16.
3. A primer mix system for diluting the RHD gene primer of claim 1 to a working concentration and preparing the primer according to a predetermined ratio, wherein the working concentration is: 100uM; the primer mixture system comprises:
0.03 to 0.05. Mu.L of primer 1, 0.03 to 0.05. Mu.L of primer 2, 0.05 to 0.07. Mu.L of primer 3, 0.05 to 0.07. Mu.L of primer 4, 0.04 to 0.06. Mu.L of primer 5, 0.04 to 0.06. Mu.L of primer 6, 0.02 to 0.04. Mu.L of primer 7, 0.02 to 0.04. Mu.L of primer 8, 0.04 to 0.06. Mu.L of primer 9, 0.04 to 0.06. Mu.L of primer 10, 0.04 to 0.06. Mu.L of primer 11, 0.04 to 0.06. Mu.L of primer 12, 0.05 to 0.07. Mu.L of primer 13, 0.05 to 0.07. Mu.L of primer 14, 0.04 to 0.06. Mu.L of primer 15, 0.04 to 0.06. Mu.L of primer 16.
4. The primer-mixed system according to claim 3, wherein the RHD gene primer is further comprising, prior to dilution to the working concentration: carrying out high-speed centrifugation treatment on the RHD gene primer;
and diluting the treated RHD gene primer to the working concentration by adopting an EB buffer solution.
5. A method for amplifying a primer mixture, comprising:
sequentially adding reagents to the primer mixture system according to claim 3 or 4: sterilizing distilled water, deoxynucleotide, primer Mix, PCR enzyme, enzyme-free sterile water and genome DNA to obtain mixed solution;
Amplifying the mixed solution to obtain an amplified product.
6. The method for amplifying a primer mixture according to claim 5,
the amount of the sterilized distilled water is as follows: 12.5. Mu.L;
the amount of deoxynucleotides is: 5. Mu.L;
the amount of the primer Mix is as follows: 0.48 μl;
the amount of the PCR enzyme is as follows: 0.5. Mu.L;
the amount of the enzyme-free sterile water is as follows: 6.52-XμL;
the amount of genomic DNA is: x μl;
the total amount of the reagent was 25. Mu.L.
7. The method of amplifying a primer mixture according to claim 5, wherein the step of amplifying the mixture to obtain amplified products comprises the following working parameters: 93-95 ℃ for 2min;97-99 ℃,12s,67-69 ℃,12min,26 cycles, 11 th cycle starting, 30s increase per cycle; 67-69 ℃ for 10min.
8. The method of amplification by a primer mixture system according to claim 5, wherein the amount of genomic DNA is: in Xμl, X is 45-55ng.
9. A method for quality testing of amplification products, comprising:
detecting the amplification product according to any one of claims 5 to 8 by agarose gel electrophoresis to obtain a detection result of whether the target gene in the amplification product is amplified; wherein, detect voltage: 150V; detection time: 50min.
10. A method of sequencing library construction comprising:
ligating the amplification product of any one of claims 5-8 to a linker to construct a library;
purifying the library;
and (3) mixing the purified library to construct the RHD gene sequencing library.
11. The sequencing library construction method according to claim 10, wherein the ligation reaction conditions are: 36-38deg.C for 40min;15-17 ℃ for 40min;64-66 deg.C for 10min.
12. A sequencing method, characterized in that the RHD gene sequencing library constructed by the construction method of the RHD gene sequencing library according to claim 10 or 11 is used for sequencing.
13. The sequencing method of claim 12, wherein the RHD gene sequencing library is sequenced using a HiFi ready mode, wherein the loading is 150pM and the average fragment size is 5000bp.
CN202310144604.2A 2023-02-21 2023-02-21 RHD gene primer, primer mixed system amplification method, amplification product quality detection method, sequencing library construction method and sequencing method Pending CN117106877A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113817725A (en) * 2021-10-15 2021-12-21 西安浩瑞基因技术有限公司 HLA gene amplification primer, kit, sequencing library construction method and sequencing method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113817725A (en) * 2021-10-15 2021-12-21 西安浩瑞基因技术有限公司 HLA gene amplification primer, kit, sequencing library construction method and sequencing method
CN113817725B (en) * 2021-10-15 2024-05-14 西安浩瑞基因技术有限公司 HLA gene amplification primer, kit, sequencing library construction method and sequencing method

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