CN117084804A - Skin wound surface constant force expansion fixer for animal experiments and application thereof - Google Patents
Skin wound surface constant force expansion fixer for animal experiments and application thereof Download PDFInfo
- Publication number
- CN117084804A CN117084804A CN202311341876.8A CN202311341876A CN117084804A CN 117084804 A CN117084804 A CN 117084804A CN 202311341876 A CN202311341876 A CN 202311341876A CN 117084804 A CN117084804 A CN 117084804A
- Authority
- CN
- China
- Prior art keywords
- skin
- piece
- fixing
- wound
- constant force
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002474 experimental method Methods 0.000 title claims abstract description 43
- 241001465754 Metazoa Species 0.000 title claims abstract description 37
- 206010072170 Skin wound Diseases 0.000 title claims abstract description 37
- 208000027418 Wounds and injury Diseases 0.000 claims abstract description 121
- 230000014759 maintenance of location Effects 0.000 claims abstract description 44
- 238000010171 animal model Methods 0.000 claims abstract description 32
- 206010050207 Skin fibrosis Diseases 0.000 claims abstract description 10
- 206010052428 Wound Diseases 0.000 claims description 115
- 230000029663 wound healing Effects 0.000 claims description 9
- 239000000853 adhesive Substances 0.000 claims description 5
- 230000001070 adhesive effect Effects 0.000 claims description 5
- 230000035876 healing Effects 0.000 abstract description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract description 5
- 239000000741 silica gel Substances 0.000 abstract description 5
- 229910002027 silica gel Inorganic materials 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 11
- 230000001575 pathological effect Effects 0.000 description 9
- 238000011160 research Methods 0.000 description 9
- 235000013372 meat Nutrition 0.000 description 7
- 239000012528 membrane Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000700159 Rattus Species 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000036558 skin tension Effects 0.000 description 4
- 241000282412 Homo Species 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000008602 contraction Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 208000032544 Cicatrix Diseases 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001969 hypertrophic effect Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 230000037387 scars Effects 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 239000010963 304 stainless steel Substances 0.000 description 1
- 238000010146 3D printing Methods 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 description 1
- 239000004830 Super Glue Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- FGBJXOREULPLGL-UHFFFAOYSA-N ethyl cyanoacrylate Chemical compound CCOC(=O)C(=C)C#N FGBJXOREULPLGL-UHFFFAOYSA-N 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000009442 healing mechanism Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
- A61B90/02—Devices for expanding tissue, e.g. skin tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dermatology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Surgical Instruments (AREA)
Abstract
The application belongs to the technical field of instruments for animal skin wound experiments, and provides a skin wound constant force expansion fixer for animal experiments and application thereof, mainly comprising a wound fixing mother board, a wound fixing daughter board and a constant force spring, wherein the wound fixing mother board comprises a fixing skin mother piece, a guide rail piece, a spring retention hook A and a suture hole, and the wound fixing daughter board comprises a fixing skin daughter piece, a sliding block piece, a spring retention hook B and a suture hole; the sliding block piece is matched with the guide rail piece and can slide on the sliding rail, and the skin fixing sub-piece is pulled away from the skin fixing parent piece through the elastic force of the constant force spring. The fixator has the functions of a silica gel splint and a fixing instrument, can apply continuous and constant fixing tension to a wound surface in animal experiments, and is suitable for being applied to the related experimental study that experimental animals are induced to form skin fibrosis in the wound surface healing process.
Description
Technical Field
The application belongs to the technical field of instruments for animal skin wound experiments, and relates to a skin wound constant force expansion fixator for animal experiments and application thereof.
Background
In modern medical research, related simulation research on human physiology or pathology is performed based on animal experiments, and is one of the most main research modes. In the related research of simulating physiological and pathological healing of human skin wound surface aiming at animal models, researchers find that the healing mechanism of skin tissue wound surface of experimental animals and human is not completely the same due to heterogeneity of species, which causes great trouble to the human skin healing process of animal modeling to simulate physiological or pathological states. Therefore, how to better simulate the healing process of human skin wound surface under physiological and pathological states, and better simulate the human body mechanical signal microenvironment under pathological states and induce the pathological continuous proliferation of callus is a very concerned and not yet fully solved problem of scientific researchers.
At present, a doughnut-shaped silica gel splint fixing method is widely used for simulating physiological healing of human skin wound surface aiming at an animal model. Specifically, a full-thickness incision is made in the skin of an experimental animal (e.g., the back of a mouse or rat): the skin tissue was cut and perforated using a biopsy punch and the adhered skin was cut and separated using auxiliary forceps and ophthalmic scissors to form a circular full-thickness wound (containing resected meat film, panniculus carnosus). The silicone splint was then applied to the skin around the wound using cyanoacrylate adhesive and the splint was further tightly secured using suture suturing (Dunn, l. Et al Murine Model of Wound health j. Vis. Exp. JoVE 50265 (2013) doi: 10.3791/50265.).
The prior art effectively avoids the promotion effect of skin shrinkage on wound healing in the animal model, so that the healing mode in the animal wound model process is more similar to the physiological process of human wound healing. However, the above-described approach fails to induce skin fibrosis in experimental animals to form hypertrophic scars (i.e., pathological conditions of wound healing in humans).
Based on this, researchers have proposed both drug-induced and mechanical stress-induced approaches to induce skin fibrosis. The medicine is mainly bleomycin injected transdermally, so that local collagen fiber is induced to be excessively deposited by inducing local inflammatory reaction and the pharmacological property of the medicine, thereby leading to skin fibrosis. Although this approach can lead to hypertrophic scars, it does not truly mimic the pathological process of skin fibrosis in humans. In contrast, the mechanical stress induction mode is theoretically considered as a promising mode.
However, related studies and experiments on mechanical stress induction are still under development. There are researchers to choose to use the existing fixing apparatuses in the field of stomatology or surgery, but there are a plurality of disadvantages of such fixing apparatuses in the simulation experiment of physiological healing of skin wound surface, for example, after the wound is caused, the skin wound surface of the experimental animal is immediately sutured by using suture, which leads to the meat membrane (panniculus carnosus) of the experimental animal such as rats, mice and the like to better participate in the healing process of the wound surface, thereby generating interference to the related analysis experiment result; in contrast, the meat membrane structure is degenerated in humans. In addition, such a fixing device can only fix and apply force to the skin wound in a short time, and the period of the related research on physiological healing of the skin wound is as long as several weeks or even months, during which the skin around the wound of the experimental animal has various physiological or pathological property changes, so that reinstallation and adjustment are usually required at intervals (usually 1-2 days) to ensure the effect of fixing and applying force to the skin wound, but on one hand, other risk factors to the experimental animal are caused intactly, even the experimental result is disturbed, and on the other hand, the complexity and labor cost of the related experiment development are improved undoubtedly.
Disclosure of Invention
The application aims to solve the problems in the prior art, and provides a skin wound constant force expansion fixer for animal experiments and application thereof, wherein the fixer has the functions of a silica gel splint and a fixing device, can apply continuous and constant fixing tension to a wound in animal experiments without disassembly and replacement after installation in the whole animal experiment period, can not influence the tension due to skin tension change of the experimental animals, and is particularly suitable for relevant experimental researches that the experimental animals are induced to form skin fibrosis in the wound healing process.
In order to achieve the above object, the present application is realized by adopting the technical scheme comprising the following technical measures.
The application provides a skin wound constant force expansion fixer for animal experiments, which mainly comprises a wound fixing motherboard, a wound fixing daughter board and a constant force spring,
the wound surface fixing mother board comprises a fixing skin mother piece with a polygonal arch bridge configuration or a fan ring configuration, the wound surface fixing son board comprises a fixing skin son piece with a polygonal arch bridge configuration or a fan ring configuration, and the fixing skin mother piece and the fixing skin son piece can be spliced to form a hollow polygon or a circular ring; the fixed skin parent piece and the fixed skin child piece are provided with a plurality of suture holes;
the wound surface fixing mother board further comprises a guide rail piece and a spring retention hook A, wherein the guide rail piece is fixedly connected with the fixed skin mother piece and provided with a guide rail facing the splicing direction of the fixed skin mother piece and the fixed skin sub piece, and the spring retention hook A is arranged at the end head of any end of the guide rail on the guide rail piece;
the wound surface fixing sub-plate further comprises a sliding block piece and a spring fixing hook B, wherein the sliding block piece is fixedly connected with the fixed skin sub-piece, is matched with the guide rail piece and can slide on the guide rail, the spring fixing hook B is arranged on the sliding block and corresponds to the spring fixing hook A, and the distance between the spring fixing hook B and the spring fixing hook A is adjustable through the sliding of the sliding block piece;
the two ends of the constant force spring are respectively provided with and fixed on the spring retention hook A and the spring retention hook B, and the skin fixation sub-piece is pulled away from the skin fixation parent piece through the elastic force of the constant force spring.
The inventor of the application summarized in long-term clinical experiments that when the existing fixing apparatus in the field of stomatology or surgery is adopted to fix the skin wound surface, frequent reinstallation and adjustment are needed, mainly because after the fixing apparatus is installed on the body of an experimental animal, the skin tension of the experimental animal changes, especially when the wound surface expands to a certain extent, the skin of the experimental animal can generate a loosening phenomenon, especially in certain pathological states, so that reinstallation and adjustment are needed at intervals (usually 1-2 days) to prevent the tension of the skin near the wound surface from being reduced, thereby causing errors of related analysis experiments and limiting the research of quantitative constant force experiments, which definitely improves the complexity and labor cost of the development of related experiments and restricts the research range.
The application aims at the defects of the prior art and the similar fixing devices, and utilizes the constant force spring to provide accurate and applied constant force so as to overcome the 'strong-weak' change of the fixing tension caused by the change of the skin tension around the wound surface, and the fixing tension can be continuously used in one experimental period without replacement and adjustment. In addition, when the fixed tension is required to be adjusted, the constant force spring can be conveniently adjusted or replaced under the condition that reinstallation is not required, so that the fixed tension can be easily adjusted.
In addition, the application has the functions of inhibiting the contraction of the meat membrane of the experimental animal and reducing the promotion of the contraction of the meat membrane to the wound healing. In animal experiment clinic, the sweet ring silica gel splint can be directly replaced to simulate the healing of human physiological state wound surface; can also inhibit contraction of the meat membrane and the wound during modeling in pathological states without changing the instrument in the middle.
In one technical scheme, the wound surface fixing motherboard comprises two guide rail pieces in strip-shaped configuration, the long ends of the guide rail pieces face the guide rail in the same direction, and the two guide rail pieces are respectively and fixedly connected to two sides of the fixing skin motherboard to form a U-shaped or M-shaped structure.
Based on the technical scheme, the number of the spring retention hooks A is at least two, and the spring retention hooks A are respectively arranged at the end heads of any one end of the guide rail on the guide rail piece.
In one preferable technical scheme, when the wound surface fixing mother board is of a U-shaped structure, at least two spring fixing hooks A are arranged on one end of the guide rail piece, which is far away from the fixed skin mother piece, respectively.
Through the technical scheme, when the guide rail parts are two or more than two, the constant force springs can be respectively arranged on two sides of the fixed skin female part, so that the force application balance is facilitated when the elastic force acts on the fixed skin sub-part. Note that when there are two or more guide rail members, the number of slide members to be matched should be the same.
In one preferable technical scheme, the number of the spring retention hooks A is at least four, and at least two spring retention hooks A are arranged on each guide rail piece, so that more accurate elastic force can be applied by installing a plurality of constant force springs.
In one preferred embodiment, the spring retention hook B has a plurality of spring retention sites to facilitate matching the proper length of the constant force spring.
In one preferable technical scheme, when the wound surface fixing motherboard comprises two guide rail pieces in long strip configuration, the ends of the two guide rail pieces, which are far away from the fixing skin motherboard, are connected through the fixing beam piece, so that the integral strength is enhanced.
In order to facilitate the installation and adjustment of the constant force spring and the like, and splice the wound surface fixing mother board and the wound surface fixing daughter board by relatively sliding through a simple structure as much as possible, when the wound surface fixing mother board and the wound surface fixing daughter board are in a spliced state, the sliding block piece penetrates through the guide rail, the spring retention hook B on the sliding block piece also penetrates through the guide rail, and the spring retention hook B and the spring retention hook A are positioned on the same surface.
In one of the technical schemes, in order to strengthen the relative sliding splicing stability of the wound surface fixing mother board and the wound surface fixing daughter board, a limiting hole is formed in the sliding block piece, and when the sliding block piece passes through the guide rail, the limiting piece is arranged in the limiting hole so as to conveniently limit the sliding of the sliding block piece.
In one of the technical schemes, in order to facilitate the fixation of the skin mother part and the fixation of the skin child part, when the fixation of the skin mother part and the fixation of the skin child part is performed to form a hollow polygon or a circular ring, dislocation or overlapping can not be generated due to elastic force, and the fixation of the skin mother part or the fixation of the skin child part is performed to limit the position during the splicing.
In one of the technical schemes, the wound surface fixing mother board is also provided with length scales which are preferably arranged on the guide rail parts and used for recording the relative distance between the fixing skin mother piece and the fixing skin child piece.
In one technical scheme, the wound surface fixing mother board and/or the wound surface fixing daughter board are/is an integrated part.
On the other hand, the application also provides an application method of the skin wound constant force expansion fixator for animal experiments, which mainly comprises the following steps:
(1) Cutting and perforating skin tissues of experimental animals, and cutting and separating adhered skin to form a circular full-layer wound;
(2) The fixed skin parent component and the fixed skin child component are stuck on the skin around the full-thickness wound by using an adhesive in a split state, so that the full-thickness wound is positioned in the hollow of the fixed skin parent component and the fixed skin child component in the split state, and the fixed skin parent component and the fixed skin child component are further stitched and fixed with the surrounding skin by using a suture line through a stitching hole;
(3) And a constant force spring is arranged, and the constant force spring exerts elastic force on the sliding block piece, so that the fixed skin sub-piece is far away from the fixed skin parent piece along the guide rail, and a fixed pulling force which is separated to two sides is exerted on the skin around the full-thickness wound.
In one embodiment, when the experimental animal simulates the tension of normal human skin, and when the applied cross section of the skin around the full wound is 0.6mm x 8mm (skin thickness x section length), the constant force spring is selected to exert an elastic force of 0.636 + -0.15N when the constant force is reached. Whereby the callus is subjected to a constant tension of about 0.132N/mm 2 。
In one embodiment, when the experimental animal is induced to form skin fibrosis during wound healing, and when the applied cross section of the skin around the full wound is 0.6mm x 8mm (skin thickness x section length), the elastic force exerted by the selected constant force spring to achieve a constant force is 7.2±0.15N. Whereby the callus is subjected to a constant tension of about 1.5N/mm 2 。
In one of the technical schemes, when the experimental animal simulates the normal healing process of human and eliminates the influence of the meat membrane of the experimental animal on the wound healing, only the constant force spring is dismounted.
It should be noted that a constant force spring is a special spring that under theoretical conditions exerts a constant force over its range of motion. The application preferably adopts an orthodontic constant force spring which is conventionally used in the field of stomatology, and always maintains the elastic force of a certain constant force within a certain stretching deformation range, and the variation or error of the elastic force is usually lower than 0.15N.
The application has the following beneficial effects:
1. the skin wound constant force expansion fixer for animal experiments provided by the application has a simple structure, has the functions of a silica gel splint and a fixing instrument, is not required to be disassembled and replaced after being installed in the whole animal experiment period process, and obviously reduces the complexity and labor cost of relevant experiment development.
2. The skin wound surface constant force expansion fixator for animal experiments can apply continuous and constant fixed tension to wound surfaces in animal experiments, can not influence the tension due to skin tension changes of experimental animals, and is particularly suitable for being applied to related experimental researches of the experimental animals induced to form skin fibrosis in the wound surface healing process.
3. According to the skin wound surface constant force expansion fixer for animal experiments, in one preferable technical scheme, the relative distance between the fixed skin parent piece and the fixed skin child piece can be recorded through the length scale arranged on the wound surface fixing parent plate, so that physiological influences and factors on the wound surface under the constant force condition can be further recorded and researched.
Drawings
Fig. 1 is a schematic structural diagram of a wound surface fixing motherboard in embodiment 1 of the present application.
Fig. 2 is a schematic view of another view angle structure of the mother board for fixing the wound surface in embodiment 1 of the present application.
Fig. 3 is a schematic view of another view angle structure of the mother board for fixing the wound surface in embodiment 1 of the present application.
Fig. 4 is a schematic structural diagram of a wound surface stator board in embodiment 1 of the present application.
Fig. 5 is a schematic view of another view angle structure of the wound surface stator board in embodiment 1 of the present application.
Fig. 6 is a schematic view of another view angle structure of the wound surface stator board in embodiment 1 of the present application.
Fig. 7 is a schematic structural diagram of the wound surface fixing mother board and the wound surface fixing daughter board in the split state in embodiment 1 of the present application.
Fig. 8 is a schematic view of another view angle structure of the wound surface fixing mother board and the wound surface fixing daughter board in the split state in embodiment 1 of the present application.
Fig. 9 is a schematic structural view of a skin wound surface constant force expansion fixator for animal experiments in accordance with embodiment 1 of the present application when the constant force spring is installed.
Fig. 10 is a schematic structural diagram of a mother board for fixing a wound surface in embodiment 2 of the present application.
Fig. 11 is a schematic structural diagram of a wound surface stator plate in embodiment 2 of the present application.
Fig. 12 is a schematic structural view of a skin wound surface constant force expansion fixator for animal experiments in accordance with embodiment 2 of the present application when the constant force spring is installed.
Fig. 13 is a schematic view of another view angle structure of the wound surface fixing mother board and the wound surface fixing daughter board in the split state in embodiment 2 of the present application.
In the figure, a wound surface fixing mother board 1, a wound surface fixing daughter board 2, a constant force spring 3, a fixed skin mother piece 4, a fixed skin child piece 5, a suture hole 6, a guide rail piece 7, a spring retention hook A8, a slide block piece 9, a spring retention hook B10, a step structure 11, a limit hole 12, a limit piece 13 and a length scale 14.
Detailed Description
For a further understanding of the present application, preferred embodiments of the application are described below in conjunction with the examples, but it should be understood that these descriptions are merely intended to illustrate further features and advantages of the application and are not limiting of the application claims. Those skilled in the art can, with the benefit of this disclosure, suitably modify the process parameters to achieve this. It is expressly noted that all such similar substitutions and modifications will be apparent to those skilled in the art, and are deemed to be included within the present application. While the structure and application of this application has been described in terms of preferred embodiments, it will be apparent to those skilled in the relevant art that the application can be practiced and practiced with modification and alteration of the structure and application described herein, or with appropriate modification and combination, without departing from the spirit and scope of the application. While the following terms are believed to be well understood by those of ordinary skill in the art, the following definitions are set forth to aid in the description of the presently disclosed subject matter. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The application should not be construed as being limited to the particular embodiments described.
Example 1
As shown in fig. 1 to 9, the skin wound surface constant force expansion fixer for animal experiments of the embodiment mainly comprises a wound surface fixing mother board 1, a wound surface fixing daughter board 2 and a constant force spring 3,
the wound surface fixing mother board 1 comprises a fixing skin mother piece 4 with a fan ring configuration, the wound surface fixing son board 2 comprises a fixing skin son piece 5 with a fan ring configuration, and the fixing skin mother piece 4 and the fixing skin son piece 5 can be spliced to form a circular ring; a plurality of suture holes 6 are formed in the fixed skin mother part 4 and the fixed skin child part 5;
the wound surface fixing mother board 2 further comprises a guide rail piece 7 and a spring retention hook A8, wherein the guide rail piece 7 is fixedly connected with the fixing skin mother piece 4 and is provided with a guide rail facing the splicing direction of the fixing skin mother piece and the fixing skin child piece;
the wound surface fixing mother board 2 comprises two guide rail pieces 7 with long strip structures, the long ends of the guide rail pieces face the same direction with the guide rails, and the two guide rail pieces 7 are respectively and fixedly connected to two sides of the fixed skin mother piece 4 to form a U-shaped structure;
the number of the spring retention hooks A8 is four, and the spring retention hooks A8 are arranged on the guide rail piece 7 in parallel in pairs, and the guide rail is far away from the end head of one end of the fixed skin mother piece 4;
the wound surface fixing sub-board 2 further comprises a sliding block piece 9 and a spring fixing hook B10, the sliding block piece 7 is fixedly connected with the fixed skin sub-piece 5, is matched with the guide rail piece 7 and can slide on the guide rail, the spring fixing hook B10 is arranged on the sliding block 9 and corresponds to the spring fixing hook A8, and the distance between the spring fixing hook B10 and the spring fixing hook A8 is adjustable through the sliding of the sliding block piece 9;
the spring retention hook B10 has a plurality of spring fixing sites to facilitate matching of the proper length of the constant force spring 3;
when the wound surface fixing motherboard 1 comprises two strip-shaped guide rail members 7, the ends of the two guide rail members, which are far away from the fixed skin motherboard, are connected through a fixing beam member so as to enhance the integral strength;
when the wound surface fixing mother board 1 and the wound surface fixing daughter board 2 are in a splicing state, the sliding block piece 9 passes through the guide rail, the spring retention hook B10 on the sliding block piece also passes through the guide rail, and the spring retention hook B10 and the spring retention hook A8 are positioned on the same surface;
the fixed skin mother part 4 is provided with a step structure 11 so as to limit when being spliced;
the two ends of the constant force spring 3 are respectively fixed on the spring retention hook A8 and the spring retention hook B10, and the fixed skin sub-piece 5 is pulled away from the fixed skin parent piece 4 by the elastic force of the constant force spring.
In this embodiment, the wound surface fixing mother board 1 and the wound surface fixing daughter board 2 are made of 304 stainless steel (source stainless steel products limited company in Xinghua city, zhejiang, china);
the constant force spring is an orthodontic spring produced by Zhejiang New medical science and technology Co., ltd, product number 9101-110-10 (Zhejiang New medical science and technology Co., ltd., zhejiang, china).
Example 2
As shown in fig. 10 to 13, the skin wound surface constant force expansion fixer for animal experiments of the embodiment mainly comprises a wound surface fixing mother board 1, a wound surface fixing daughter board 2 and a constant force spring 3,
the wound surface fixing mother board 1 comprises a fixing skin mother piece 4 with a fan ring configuration, the wound surface fixing son board 2 comprises a fixing skin son piece 5 with a fan ring configuration, and the fixing skin mother piece 4 and the fixing skin son piece 5 can be spliced to form a circular ring; a plurality of suture holes 6 are formed in the fixed skin mother part 4 and the fixed skin child part 5;
the wound surface fixing mother board 2 further comprises a guide rail piece 7 and a spring retention hook A8, wherein the guide rail piece 7 is fixedly connected with the fixing skin mother piece 4 and is provided with a guide rail facing the splicing direction of the fixing skin mother piece and the fixing skin child piece;
the wound surface fixing mother board 2 comprises two guide rail pieces 7 with long strip structures, the long ends of the guide rail pieces face the same direction with the guide rails, and the two guide rail pieces 7 are respectively and fixedly connected to two sides of the fixed skin mother piece 4 to form a U-shaped structure;
the number of the spring retention hooks A8 is four, and the spring retention hooks A8 are arranged on the guide rail piece 7 in parallel in pairs, and the guide rail is far away from the end head of one end of the fixed skin mother piece 4;
the wound surface fixing sub-board 2 further comprises a sliding block piece 9 and a spring fixing hook B10, the sliding block piece 7 is fixedly connected with the fixed skin sub-piece 5, is matched with the guide rail piece 7 and can slide on the guide rail, the spring fixing hook B10 is arranged on the sliding block 9 and corresponds to the spring fixing hook A8, and the distance between the spring fixing hook B10 and the spring fixing hook A8 is adjustable through the sliding of the sliding block piece 9;
the spring retention hook B10 has a plurality of spring fixing sites to facilitate matching of the proper length of the constant force spring 3;
when the wound surface fixing motherboard 1 comprises two strip-shaped guide rail members 7, the ends of the two guide rail members, which are far away from the fixed skin motherboard, are connected through a fixing beam member so as to enhance the integral strength;
when the wound surface fixing mother board 1 and the wound surface fixing daughter board 2 are in a splicing state, the sliding block piece 9 passes through the guide rail, the spring retention hook B10 on the sliding block piece also passes through the guide rail, and the spring retention hook B10 and the spring retention hook A8 are positioned on the same surface;
the fixed skin mother part 4 is provided with a step structure 11 so as to limit when being spliced;
the two ends of the constant force spring 3 are respectively fixed on the spring retention hook A8 and the spring retention hook B10, and the fixed skin sub-piece 5 is pulled away from the fixed skin parent piece 4 by the elastic force of the constant force spring.
The limiting holes 12 are formed in the sliding block pieces 9, and when the sliding block pieces 9 pass through the guide rails, limiting pieces 13 are arranged in the limiting holes 12 to conveniently limit sliding of the sliding block pieces.
The wound surface fixing mother board 1 is also provided with a length scale 14, and the length scale 14 is arranged on the guide rail piece 7 and is used for recording the relative distance between the fixing skin mother piece 4 and the fixing skin child piece 5.
In this embodiment, the wound surface fixing motherboard 1 and the wound surface fixing daughter board 2 are all integrally formed pieces obtained by a 3D printing technology.
Application example 1
The application example is an application method of the skin wound constant force expansion fixator for animal experiments, which is shown in the embodiment 2, wherein the experimental animals simulate the tension of normal human skin and mainly comprises the following steps:
(1) Cutting and perforating skin tissue of experimental animal (rat), cutting and separating adhered skin to form a round full-layer wound, wherein the stress cross section of the skin around the full-layer wound is 0.6mm×8mm (skin thickness×section length);
(2) The fixed skin parent component and the fixed skin child component are stuck on the skin around the full-thickness wound by using an adhesive in a split state, so that the full-thickness wound is positioned in the hollow of the fixed skin parent component and the fixed skin child component in the split state, and the fixed skin parent component and the fixed skin child component are further stitched and fixed with the surrounding skin by using a suture line through a stitching hole;
(3) The constant force spring is installed, the constant force spring applies elastic force to the sliding block piece, so that the fixed skin sub piece is far away from the fixed skin parent piece along the guide rail, and accordingly fixed tensile force which is separated to two sides is applied to the skin around the full-thickness wound, and the elastic force applied when the constant force spring reaches the constant force is 0.636+/-0.15N through selecting the constant force spring and adjusting the spring fixing points of the constant force spring.
Application example 2
The application example is an application method of the skin wound constant force expansion fixator for animal experiments, which is shown in the embodiment 2, wherein the experimental animals are induced to form skin fibrosis in the wound healing process, and the application method mainly comprises the following steps of:
(1) Cutting and perforating skin tissue of experimental animal (rabbit), cutting and separating adhered skin to form a round full-layer wound, wherein the stress cross section of the skin around the full-layer wound is 0.6mm×8mm (skin thickness×section length);
(2) The fixed skin parent component and the fixed skin child component are stuck on the skin around the full-thickness wound by using an adhesive in a split state, so that the full-thickness wound is positioned in the hollow of the fixed skin parent component and the fixed skin child component in the split state, and the fixed skin parent component and the fixed skin child component are further stitched and fixed with the surrounding skin by using a suture line through a stitching hole;
(3) The constant force spring is installed, the constant force spring applies elastic force to the sliding block piece, so that the fixed skin sub piece is far away from the fixed skin parent piece along the guide rail, and accordingly fixed tensile force which is separated to two sides is applied to the skin around the full-thickness wound, and the elastic force applied when the constant force spring reaches the constant force is 7.2+/-0.15N through selecting the constant force spring and adjusting the spring fixing points of the constant force spring.
Application example 3
The application example is an application method of the skin wound constant force expansion fixator for animal experiments, which is shown in the embodiment 2 and mainly comprises the following steps:
(1) Cutting and perforating skin tissue of an experimental animal (rat), and cutting and separating adhered skin to form a circular full-layer wound;
(2) The fixed skin parent component and the fixed skin child component are stuck on the skin around the full-thickness wound by using an adhesive in a split state, so that the full-thickness wound is positioned in the hollow of the fixed skin parent component and the fixed skin child component in the split state, and the fixed skin parent component and the fixed skin child component are further stitched and fixed with the surrounding skin by using a suture line through a stitching hole;
(3) The constant force spring is installed, the constant force spring exerts elastic force on the sliding block piece, the fixed skin sub piece is far away from the fixed skin parent piece along the guide rail, and accordingly fixed tensile force which is separated to two sides is exerted on the skin around the full-thickness wound, and the elastic force of the constant force spring in the stretching deformation range specification is similar to the constant force by selecting the constant force spring and adjusting the spring fixing points of the constant force spring.
(4) The relative distance between the fixed skin parent piece and the fixed skin child piece is recorded daily through the length scale arranged on the wound surface fixed parent plate and is used as an experimental recording parameter.
The foregoing examples are illustrative of the present application and are not intended to be limiting, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principles of the application are intended to be equivalent and are within the scope of the present application.
Claims (10)
1. The skin wound surface constant force expansion fixer for animal experiments is characterized by mainly comprising a wound surface fixing mother board, a wound surface fixing daughter board and a constant force spring,
the wound surface fixing mother board comprises a fixing skin mother piece with a polygonal arch bridge configuration or a fan ring configuration, the wound surface fixing son board comprises a fixing skin son piece with a polygonal arch bridge configuration or a fan ring configuration, and the fixing skin mother piece and the fixing skin son piece can be spliced to form a hollow polygon or a circular ring; the fixed skin parent piece and the fixed skin child piece are provided with a plurality of suture holes;
the wound surface fixing mother board further comprises a guide rail piece and a spring retention hook A, wherein the guide rail piece is fixedly connected with the fixed skin mother piece and provided with a guide rail facing the splicing direction of the fixed skin mother piece and the fixed skin sub piece, and the spring retention hook A is arranged at the end head of any end of the guide rail on the guide rail piece;
the wound surface fixing sub-plate further comprises a sliding block piece and a spring fixing hook B, wherein the sliding block piece is fixedly connected with the fixed skin sub-piece, is matched with the guide rail piece and can slide on the guide rail, the spring fixing hook B is arranged on the sliding block and corresponds to the spring fixing hook A, and the distance between the spring fixing hook B and the spring fixing hook A is adjustable through the sliding of the sliding block piece;
the two ends of the constant force spring are respectively provided with and fixed on the spring retention hook A and the spring retention hook B, and the skin fixation sub-piece is pulled away from the skin fixation parent piece through the elastic force of the constant force spring.
2. The skin wound constant force expansion fixator for animal experiments according to claim 1 wherein: the wound surface fixing motherboard comprises two guide rail pieces in strip-shaped configuration, the long ends of the guide rail pieces face the guide rail in the same direction, and the two guide rail pieces are respectively and fixedly connected to two sides of the skin fixing motherboard to form a U-shaped or M-shaped structure.
3. The skin wound constant force expansion fixator for animal experiments according to claim 2 wherein: when the wound surface fixing mother board is of a U-shaped structure, at least two spring retention hooks A are arranged on the guide rail piece, and the guide rail is far away from one end of the fixed skin mother piece.
4. The skin wound constant force expansion fixator for animal experiments according to claim 1 wherein: the spring retention hook B has a plurality of spring fixation sites.
5. The skin wound constant force expansion fixator for animal experiments according to claim 1 wherein: when the wound surface fixing mother board and the wound surface fixing daughter board are in a splicing state, the sliding block piece penetrates through the guide rail, the spring retention hook B on the sliding block piece also penetrates through the guide rail, and the spring retention hook B and the spring retention hook A are located on the same surface.
6. The skin wound constant force expansion fixator for animal experiments according to claim 1 wherein: when the wound surface fixing motherboard comprises two strip-shaped guide rail pieces, the ends of the two guide rail pieces, which are far away from the fixed skin motherboard, are connected through a fixing beam piece.
7. The skin wound constant force expansion fixator for animal experiments according to claim 1 wherein: the constant force spring is an orthodontic constant force spring.
8. The use of the skin wound constant force expansion fixator for animal experiments as claimed in claim 1 in animal skin wound experiments.
9. The application according to claim 8, characterized in that it comprises the following steps:
(1) Cutting and perforating skin tissues of experimental animals, and cutting and separating adhered skin to form a circular full-layer wound;
(2) The fixed skin parent component and the fixed skin child component are stuck on the skin around the full-thickness wound by using an adhesive in a split state, so that the full-thickness wound is positioned in the hollow of the fixed skin parent component and the fixed skin child component in the split state, and the fixed skin parent component and the fixed skin child component are further stitched and fixed with the surrounding skin by using a suture line through a stitching hole;
(3) And a constant force spring is arranged, and the constant force spring exerts elastic force on the sliding block piece, so that the fixed skin sub-piece is far away from the fixed skin parent piece along the guide rail, and a fixed pulling force which is separated to two sides is exerted on the skin around the full-thickness wound.
10. The use according to claim 9, characterized in that:
when the experimental animal simulates the tension of normal human skin, and when the applied cross section of the skin around the full wound is 0.6mm×8mm, the elastic force applied when the selected constant force spring reaches a constant force is 0.636±0.15N;
when experimental animals were induced to form skin fibrosis during wound healing, and when the applied skin cross section around the full wound was 0.6mm x 8mm, the elastic force exerted by the selected constant force spring to reach a constant force was 7.2±0.15N.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311341876.8A CN117084804B (en) | 2023-10-17 | 2023-10-17 | Skin wound surface constant force expansion fixer for animal experiments and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311341876.8A CN117084804B (en) | 2023-10-17 | 2023-10-17 | Skin wound surface constant force expansion fixer for animal experiments and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117084804A true CN117084804A (en) | 2023-11-21 |
| CN117084804B CN117084804B (en) | 2023-12-19 |
Family
ID=88772034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311341876.8A Active CN117084804B (en) | 2023-10-17 | 2023-10-17 | Skin wound surface constant force expansion fixer for animal experiments and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117084804B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2183998A1 (en) * | 1995-08-30 | 1997-03-01 | Wayne Porter | Ligating clip having ramp-shaped vessel clamping members and tool for applying same |
| CN111643215A (en) * | 2020-07-07 | 2020-09-11 | 华中科技大学同济医学院附属协和医院 | Skin fixator for wound experimental model and using method thereof |
| CN111820973A (en) * | 2020-07-29 | 2020-10-27 | 广东省人民医院 | Limb skin scar tension adjusting combination device |
| CN112535536A (en) * | 2021-01-20 | 2021-03-23 | 周征兵 | Intelligent control skin stretching closer |
| CN213098092U (en) * | 2020-06-17 | 2021-05-04 | 南方医科大学南方医院 | Telescopic skin stretching device |
| CN213283431U (en) * | 2020-05-13 | 2021-05-28 | 王东强 | Prevent pressing wound type skin and lead a ware |
| CN112869813A (en) * | 2021-01-06 | 2021-06-01 | 四川大学华西医院 | Four-limb skin scar tension adjusting combined system and method |
| CN214967043U (en) * | 2020-12-24 | 2021-12-03 | 华中科技大学同济医学院附属协和医院 | Skin fixer for animal wound model |
| CN217040412U (en) * | 2022-01-19 | 2022-07-26 | 中国人民解放军空军军医大学 | Full-layer skin injury modeling device for experimental animals |
| CN219332066U (en) * | 2022-11-16 | 2023-07-14 | 南方医科大学皮肤病医院（广东省皮肤病医院、广东省皮肤性病防治中心、中国麻风防治研究中心） | Animal wound surface fixer for experimental model |
-
2023
- 2023-10-17 CN CN202311341876.8A patent/CN117084804B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2183998A1 (en) * | 1995-08-30 | 1997-03-01 | Wayne Porter | Ligating clip having ramp-shaped vessel clamping members and tool for applying same |
| CN213283431U (en) * | 2020-05-13 | 2021-05-28 | 王东强 | Prevent pressing wound type skin and lead a ware |
| CN213098092U (en) * | 2020-06-17 | 2021-05-04 | 南方医科大学南方医院 | Telescopic skin stretching device |
| CN111643215A (en) * | 2020-07-07 | 2020-09-11 | 华中科技大学同济医学院附属协和医院 | Skin fixator for wound experimental model and using method thereof |
| CN111820973A (en) * | 2020-07-29 | 2020-10-27 | 广东省人民医院 | Limb skin scar tension adjusting combination device |
| CN214967043U (en) * | 2020-12-24 | 2021-12-03 | 华中科技大学同济医学院附属协和医院 | Skin fixer for animal wound model |
| CN112869813A (en) * | 2021-01-06 | 2021-06-01 | 四川大学华西医院 | Four-limb skin scar tension adjusting combined system and method |
| CN112535536A (en) * | 2021-01-20 | 2021-03-23 | 周征兵 | Intelligent control skin stretching closer |
| CN217040412U (en) * | 2022-01-19 | 2022-07-26 | 中国人民解放军空军军医大学 | Full-layer skin injury modeling device for experimental animals |
| CN219332066U (en) * | 2022-11-16 | 2023-07-14 | 南方医科大学皮肤病医院（广东省皮肤病医院、广东省皮肤性病防治中心、中国麻风防治研究中心） | Animal wound surface fixer for experimental model |
Non-Patent Citations (1)
| Title |
|---|
| 党正杰;张宝泉;王守峰;杨新刚;高伟;: "实用新型皮肤牵拉器的临床应用", 中国组织工程研究, no. 43, pages 165 - 170 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117084804B (en) | 2023-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Emadi et al. | Stiffness of the gerbil basilar membrane: radial and longitudinal variations | |
| Eysholdt et al. | Vocal fold vibration irregularities caused by different types of laryngeal asymmetry | |
| Haug et al. | Does plate adaptation affect stability? A biomechanical comparison of locking and nonlocking plates | |
| Koh et al. | Excursion is important in regulating sarcomere number in the growing rabbit tibialis anterior | |
| Aalto et al. | Large scale data acquisition of simultaneous MRI and speech | |
| Iannotti et al. | Effects of plate location and selection on the stability of midshaft clavicle osteotomies: a biomechanical study | |
| Namasivayam et al. | Investigating speech motor practice and learning in people who stutter | |
| Stathopoulos et al. | Respiratory and laryngeal measures of children during vocal intensity variation | |
| Maytag et al. | Use of the rabbit larynx in an excised larynx setup | |
| CN107210002B (en) | Wound closure simulation model for in-vitro anatomical tissue sample | |
| Schumacher et al. | Motor functions in trumpet playing—a real-time MRI analysis | |
| Regner et al. | The effect of vocal fold adduction on the acoustic quality of phonation: ex vivo investigations | |
| CN117084804B (en) | Skin wound surface constant force expansion fixer for animal experiments and application thereof | |
| Düring et al. | In situ vocal fold properties and pitch prediction by dynamic actuation of the songbird syrinx | |
| Riede et al. | The acoustic effect of vocal tract adjustments in zebra finches | |
| Di Puccio et al. | Fracture healing assessment based on impact testing: In vitro simulation and monitoring of the healing process of a tibial fracture with external fixator | |
| Elemans et al. | Smooth operator: avoidance of subharmonic bifurcations through mechanical mechanisms simplifies song motor control in adult zebra finches | |
| Alipour et al. | Measures of spectral slope using an excised larynx model | |
| Rihkanen et al. | Videostroboscopic assessment of unilateral vocal fold paralysis after augmentation with autologous fascia | |
| Kelleher et al. | Use of a static magnetic field to promote recovery after peripheral nerve injury | |
| Sperringer et al. | In vitro assays to determine skeletal muscle physiologic function | |
| Mencio et al. | Contributions of rapid neuromuscular transmission to the fine control of acoustic parameters of birdsong | |
| CN117323054A (en) | Skin wound constant force push-pull rectangular fixer for animal experiments and application thereof | |
| Wales et al. | Minimally invasive laser vibrometry (MIVIB) with a floating mass transducer–a new method for objective evaluation of the middle ear demonstrated on stapes fixation | |
| Döllinger et al. | Juvenile ovine ex vivo larynges: phonatory, histologic, and micro CT based anatomic analyses |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |