CN117017842A - Yeast fermentation product filtrate with relieving and repairing effects and preparation method thereof - Google Patents
Yeast fermentation product filtrate with relieving and repairing effects and preparation method thereof Download PDFInfo
- Publication number
- CN117017842A CN117017842A CN202311156570.5A CN202311156570A CN117017842A CN 117017842 A CN117017842 A CN 117017842A CN 202311156570 A CN202311156570 A CN 202311156570A CN 117017842 A CN117017842 A CN 117017842A
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- Prior art keywords
- fermentation
- fermentation product
- yeast
- product filtrate
- soothing
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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Abstract
The invention relates to a saccharomycete fermentation product filtrate with a relieving and repairing effect and a preparation method thereof, belonging to the field of cosmetic preparations. The invention provides a saccharomycete fermentation product filtrate with a relieving and repairing effect, which comprises the following components: the compound saccharomycete seed liquid and the liquid fermentation culture medium comprise: soy protein, modified wort, carbon source. The yeast fermentation product filtrate provided by the invention is rich in polysaccharide, amino acid, antioxidant substances and various active ingredients, and has good anti-inflammatory and skin repairing effects.
Description
Technical Field
The invention belongs to the field of cosmetic preparations, and particularly relates to a saccharomycete fermentation product filtrate with a relieving and repairing effect and a preparation method thereof.
Background
The cosmetic skin care products have been developed from chemical cosmetology and plant cosmetology to biological cosmetology and gene cosmetology, and the addition of bioactive substances to the skin care products has become a trend in the beauty community. Bioactive substances are substances with low effective concentration but extremely high activity, and play a role in biological regulation of various cell physiological functions and metabolic activities.
In daily care, bioactive substances are a very popular component of skin care at present, can control or regulate the skin aging process, protect damaged skin and delay skin aging, and have important significance in maintaining the structure and function of normal skin and maintaining the normal physiological activities and metabolism of organisms.
Although some strain fermentation technologies and fermentation products have been disclosed in the prior art, the shortcomings of poor antioxidant effect, poor anti-inflammatory effect, poor skin repair effect and the like still exist in the prior art for the application of a single fermentation strain in cosmetics; the multi-strain fermentation has the problems of difficult collaborative symbiotic fermentation, difficult control of fermentation conditions, low efficiency of fermentation products, insufficient nutrition factors of the fermentation products and the like, so that the application effect is poor.
Therefore, the development of the composite strain fermentation filtrate which has the advantages of good relief, skin injury repair, anti-inflammation and simple and easily controlled fermentation preparation process is the key point of the research of the invention.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a composite saccharomycete fermentation product filtrate for effectively inhibiting inflammatory factors and promoting the rapid repair of skin cells and a preparation method thereof.
In order to achieve the above purpose, the invention provides a yeast fermentation product filtrate with a soothing and repairing effect, which comprises the following components:
compounding a saccharomycete seed solution;
a liquid fermentation medium;
the inoculation amount of the compound saccharomycete seed liquid is 5-10v/v% of the liquid fermentation culture medium;
the liquid fermentation medium comprises the following components;
5-10g/L of soybean protein;
5-10g/L of modified wort;
10-30g/L of carbon source.
Further, the preparation method of the modified wort comprises the following steps: grinding malt, adding water, heating in boiling water for 20-40min, cooling to room temperature, adding glucose isomerase, reacting for 1-2 hr, heating to deactivate glucose isomerase, and filtering with gauze to obtain modified wort.
Preferably, the glucose isomerase has an enzyme activity of 50-100U/g, and the glucose isomerase is added in an amount of at least 0.03 parts by mass per 5 parts by mass of malt; when the malt is ground and added with water, the water addition amount is at least 5 times of the malt by mass.
The soybean is rich in vitamins and minerals including vitamin B group, vitamin E, potassium, magnesium, iron, zinc, etc. The soybean also comprises main active ingredients such as gamma-aminobutyric acid (GABA), flavone, polyphenol, etc. The isoflavone in the soybean has unique functions of resisting oxidization, resisting cholesterol, resisting hyperlipidemia, resisting bacteria, diminishing inflammation and the like.
The invention discovers that the fermentation filtrate obtained by directly taking soybean as a fermentation raw material contains allergen which can cause skin anaphylactic reaction and skin irritation, so that the invention adopts soybean protein to replace soybean, reduces the occurrence of anaphylactic reaction and improves the safety of skin care.
The invention adopts the soybean protein and the modified wort as the components of the liquid fermentation medium, and the synergistic fermentation of the soybean protein and the modified wort has better effects of diminishing inflammation, resisting oxidation and promoting the metabolic migration of keratinocytes than the fermentation of a single component, so that the contents of polypeptide, polyphenol and isoflavone in the fermentation product are improved, and the effects of resisting oxidation, resisting bacteria and diminishing inflammation are realized.
Further, the carbon source is at least one of sucrose, maltose, glucose, fructose, lactose, galactose and mannitol.
Further, the preparation method of the compound saccharomycete seed liquid comprises the following steps:
(1) Inoculating pichia pastoris in an activation culture medium, and placing the pichia pastoris in a shaking table at the temperature of 25-30 ℃ and the shaking culture speed of 250-300r/min for 24-48 hours;
(2) Inoculating the saccharomyces boulardii into an activation culture medium, and placing the culture medium into a shaking table for shake culture for 24-48 hours at the temperature of 28-30 ℃ and the speed of 180-220r/min, wherein the saccharomyces boulardii seed liquid;
(3) The pichia pastoris seed liquid and the Blakeslea pastoris seed liquid are compounded according to the volume ratio of (2-3): 1, and the compounded yeast seed liquid is obtained.
Preferably, the inoculation amount of the pichia pastoris in the step (1) is (1.2-1.3) multiplied by 10 6 CFU/ml; the inoculation amount of the saccharomyces boulardii in the step (2) is (1.0-1.5) multiplied by 10 6 CFU/ml。
Further, the activation medium is added with the following basic medium: glucose 18-22g/L, yeast extract 9-11g/L, peptone 18-22g/L, wherein the mass ratio of glucose, yeast extract and peptone is (1.7-2.4): 1 (1.7-2.4).
Preferably, the mass ratio of glucose, yeast extract and peptone is 2:1:2.
The pichia pastoris and the Blakeslea are adopted as combined fermentation strains, and the cell metabolites of the strains, such as polysaccharide, protein, polypeptide and other nutrient substances, have higher yield and better fermentation effect than single strain; the mixed strain fermentation product has the effects of regulating and balancing skin and regulating immune function; the polysaccharide, vitamin and other molecules and mixed bacterial cytoplasmic metabolites in the fermentation product have certain moisturizing and antioxidation effects, and can prevent the damage caused by external stimulus such as ultraviolet rays and the like and promote the repair of damaged DNA. Further improves the antioxidation effect by fermenting with the soybean protein and the modified wort in the fermentation medium.
According to the invention, glucose isomerase is utilized to isomerise and catalyze glucose in wort to generate fructose, so that the content of fructose in wort is increased, and the fructose is a main carbon source and nutrition intake of pichia pastoris and saccharomyces boulardii combined fermentation, and has the effect of promoting the stable growth of compound fermentation bacteria liquid; fructose can regulate the metabolic processes of pichia pastoris and branchia yeast, and the metabolic modes of fructose can be changed due to the difference between the metabolic pathways of fructose and glucose, so that the synthesis and secretion of nutritional factors in fermentation products are affected.
The invention also provides a preparation method of the saccharomycete fermentation product filtrate with the relieving and repairing effects, which comprises the following steps of:
(1) Weighing soybean protein, modified wort and carbon source according to the formula amount to prepare a liquid fermentation culture medium;
(2) Inoculating the compound saccharomycete seed liquid with the formula amount into a liquid fermentation medium for fermentation to obtain fermentation liquor;
(3) Centrifuging, filtering and sterilizing the fermentation liquor obtained in the step (2) to obtain a saccharomycete fermentation product filtrate with a relieving and repairing effect.
Further, the fermentation culture conditions of the step (2) are as follows:
culturing for 0-24 h: the temperature is 28-30 ℃, the pH is 5.0-6.0, and the rpm is 180-200;
the culture time is 25-72 h: the temperature is 26-28deg.C, pH is 5.5-7.0, and 200-220rpm.
Further, the centrifugal force in the step (3) is set to 8000-10000rpm, and the centrifugal time is 10-20min.
Further, the filtering and sterilizing in the step (3) is as follows: filtering and sterilizing by a polyether sulfone filter core with the diameter of less than or equal to 0.22 mu m.
The preparation method of the yeast fermentation product filtrate can decompose proteins, fats, various bioactive substances and the like rich in soybean protein and malt into substances which are easy to be absorbed by skin, such as micromolecular saccharides, peptides, amino acids, organic acids and the like. The preparation method can retain the beneficial functional components such as amino acid in the soybean protein and the like for skin to a great extent; meanwhile, phenolic substances, VB vitamins, mineral substances and the like generated by fermenting malt by saccharomycetes can strengthen skin resistance, and are beneficial to protecting skin health.
The invention has the beneficial effects that:
1. the saccharomycete fermentation liquor provided by the invention is rich in polysaccharide, amino acid, antioxidant substances and various active ingredients, and can provide lasting moisturizing effect and increase the moisture content of skin; repairing damaged skin tissue, reducing skin problems such as inflammation, sensitivity, redness, and the like; meanwhile, the saccharomycete fermentation liquor can regulate the grease secretion of the skin.
2. The saccharomycete fermentation filtrate provided by the invention has obvious inhibition effect on inflammatory factor TNF-alpha. The inhibition rate of the yeast fermentation product filtrate with the concentration of 3% (v/v), 1% (v/v) and 0.5% (v/v) on inflammatory factor TNF-alpha is as high as 32.872%, 25.258% and 20.514%, which shows that the yeast fermentation product filtrate has a better relieving effect.
3. The yeast fermentation filtrate provided by the invention can promote the migration capability of keratinocytes. Yeast fermentation product filtrate with concentration of 1% (v/v), 0.5% (v/v) and 0.1% (v/v) has significant increase of keratinocyte migration area, mobility of 29.696%, 21.147% and 20.680%, which shows that the product filtrate has good repairing effect.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific examples. It will be appreciated by persons skilled in the art that the specific embodiments described herein are for purposes of illustration only and are not intended to be limiting.
The test methods used in the examples and comparative examples are conventional methods unless otherwise specified; materials, reagents, and the like used, unless otherwise specified, are commercially available; the percentages mentioned in the examples and comparative examples are percentages by mass, unless otherwise specified.
The pichia pastoris (pichia pastoris) is purchased from China general microbiological culture collection center.
The Saccharomyces boulardii (Saccharomyces boulardii, S.boulardii) of the invention was purchased from Yunnan Boshiao Biotechnology Co.
Preparation of modified wort:
step (1), weighing a certain amount of raw materials according to the table 1.
Step (2), cleaning the malt with the fresh bud length of 2-5 cm; grinding, adding water while grinding, and heating in boiling water (95deg.C or more) for 20-40min under water bath, and stirring to obtain malt slurry.
And (3) cooling the malt syrup to room temperature, adding glucose isomerase for reaction for 1-2h, heating to inactivate the glucose isomerase, stopping the reaction, and filtering with 3 layers of gauze to obtain modified wort.
TABLE 1
Note that: the raw materials in the table are in parts by mass, "-indicates that no additive is added.
Preparing a compound saccharomycete seed solution:
step (1), preparation of an activation medium: raw materials were weighed according to table 2, and each raw material component was dissolved in a beef extract peptone basal medium to obtain an activated medium.
TABLE 2 raw materials for activation Medium
| Raw materials | Activation medium (1) | Activation medium (2) | Activation medium (3) | Activation medium (4) |
| Glucose (g/L) | 18 | 22 | 18 | 22 |
| Yeast extract (g/L)) | 9 | 11 | 11 | 9 |
| Peptone (g/L) | 18 | 22 | 18 | 22 |
Step (2), diluting the commercial pichia pastoris to a certain number of viable bacteria according to the culture parameters of the table 3, inoculating the commercial pichia pastoris into an activation culture medium, and placing the activation culture medium at a certain temperature, and shake culturing for a period of time by a shaking table to obtain pichia pastoris seed liquid;
TABLE 3 culture parameters
Step (3), according to the culture parameters of Table 4, diluting the commercial saccharomyces boulardii to a certain number of viable bacteria, inoculating the commercial saccharomyces boulardii into an activation culture medium, placing the activation culture medium at a certain temperature, and shake culturing for a certain time by a shaking table to obtain a saccharomyces boulardii seed solution;
TABLE 4 culture parameters
And (4) compounding the pichia pastoris seed solution and the saccharomyces boulardii seed solution prepared in the step (2) and the step (3) according to the volume ratio of table 5 to obtain the compound saccharomyces boulardii seed solution.
TABLE 5 composition of compounded Yeast seed liquid
Note that: the addition amount in the table is volume fraction, "-indicates no addition.
Preparation of examples and comparative examples:
step (1), weighing all raw material components according to a table 6, and preparing a liquid fermentation medium by taking water as a solvent;
TABLE 6 liquid fermentation Medium Components
Note that: "-indicates no addition.
Step (2), inoculating the compound saccharomycete seed liquid according to the volume parts of table 7 into a liquid fermentation medium for fermentation, and carrying out two fermentation stages, namely a first stage of fermentation for 24 hours and a second stage of fermentation for 48 hours to obtain fermentation liquor;
and (3) centrifuging, filtering and sterilizing the fermentation liquor obtained in the step (2) to obtain the examples and the comparative examples.
Table 7 preparation parameters of examples and comparative examples
Note that: the addition amount of the components in the table is in parts by volume, and the term "-indicates that the components are not added.
Human skin patch test:
the 5 examples and 3 comparative examples prepared above were referred to the human skin patch test in 2022 cosmetic safety technical Specification.
Skin reactions were observed as standard at 30min (after the disappearance of the indentations), 24h and 48h, respectively, and the observations were recorded, see table 8.
TABLE 8 human safety test results
| Numbering device | 30min | 24h | 48h |
| Example 1 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
| Example 2 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
| Example 3 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
| Example 4 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
| Example 5 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
| Comparative example 1 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
| Comparative example 2 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
| Comparative example 3 | Grade 0, 30 | Grade 0, 30 | Grade 0, 30 |
Performance test:
inflammatory factor inhibition assay
Sample to be measured: a certain amount of the yeast fermentation product filtrates of examples 1 to 5 and comparative examples 1 to 3 were diluted to 0.5v/v%, 1v/v% and 3v/v%, respectively, with physiological saline.
The experimental method comprises the following steps: RAW264.7 macrophages were used as subjects to establish a model of cellular inflammation by stimulating cells with lipopolysaccharide LPS (bacterial endotoxin). Seeding macrophage cells into 12-well plate, culturing in incubator at 37deg.C and 5% CO 2 Incubation was carried out at aeration for 24h, and dilutions (0.5 v/v%, 1v/v%, 3 v/v%) of examples 1 to 5 and comparative examples 1 to 3 were added, respectively, and LPS (1. Mu.g/mL) was added after 2h, designated T 1 The group to which LPS alone was added without adding the diluent was designated as T Total (S) Without addition of LPS onlyThe group to which the diluent was added was designated T 0 Stimulating for 24 hours, collecting supernatant, centrifuging, and detecting. The level of proinflammatory inflammatory factor TNF-alpha release from RAW264.7 was analyzed using ELISA kit.
TNF- α inhibition was calculated from the following formula:
TNF-alpha inhibition (%) = [ (T) Total (S) -T 1 )/(T Total (S) -T 0 )]×100％。
The higher the TNF-alpha inhibition, the better the anti-inflammatory effect of the yeast fermentation product filtrate. The TNF- α inhibition test results are shown in Table 9.
TABLE 9 TNF-alpha inhibition
Analysis of results:
compared with comparative examples 1-3, examples 1-5 confirm that the yeast fermentation product filtrate provided by the invention has obvious inhibition effect on inflammatory factor TNF-alpha. The concentration of the filtrate of the saccharomycete fermentation product in the embodiment 3 is 3% (v/v), 1% (v/v) and 0.5% (v/v), the inhibition rate of inflammatory factor TNF-alpha is as high as 32.872%, 25.258% and 20.514%, and the filtrate has good anti-inflammatory and relieving effects.
Skin keratinocyte scratch healing promotion effect experiment
Sample to be measured: a certain amount of the yeast fermentation product filtrates of examples 1 to 5 and comparative examples 1 to 3 were diluted with physiological saline to a certain concentration (concentration gradient of 1v/v%, 0.5v/v%, 0.1 v/v%). Blank control: physiological saline. Each sample to be tested is provided with a concentration of 1v/v%, 0.5v/v% and 0.1v/v%, three parallel controls are arranged for each concentration, and the results of the three parallel controls are averaged.
Experimental protocol: haCat cells were grown at 4X 10 4 Inoculating into cell plug-in unit, culturing in cell incubator until cell density is about 80%, removing liquid in cell plug-in unit and 6-well plate, adding 2ml (concentration gradient of 1, 0.5, 0.1 v/v%) of each sample to be tested, culturing for 24 hr, photographing for 0 hr and 24 hr, and measuring with imageJ meterThe cell area was calculated and the corresponding mobility calculated. The influence of the sample to be tested on the migration of the skin keratinocytes, and the cell scratch test is a simple, convenient and quick method for measuring the migration movement and the repair capability of the cells, and is similar to an in-vitro wound healing model. Detecting the mobility of the cells can reflect the barrier repairing capability of the cells, if the mobility is large, the barrier repairing capability of the sample to be detected is good, and if the mobility is small, the barrier repairing capability of the sample to be detected is poor.
The result calculation formula: mobility= (0 h area-24 h area)/0 h area, relative mobility = sample mobility-blank mobility.
The test results are shown in Table 10.
TABLE 10 effects of examples 1-5 and comparative examples 1-3 on skin keratinocyte mobility
Analysis of results:
examples 1-5 significantly promote the migration of skin keratinocytes at concentrations of 0.1v/v%, 0.5v/v% and 1v/v%, wherein example 3 showed a greater increase in keratinocyte migration area at concentrations of 1v/v%, 0.5v/v% and 0.1v/v% with greater relative mobilities of 29.696%, 21.147% and 20.680%; example 3 it was confirmed that wort modified by glucose isomerase has effects of promoting the production of nutrient factors of the filtrate of yeast fermentation product and improving the effect as compared with comparative examples 1 to 3; compared with single fermentation seed liquid, the compound fermentation seed liquid has a synergistic effect, and the repairing function of the fermentation filtrate on the skin is improved.
Sample cell grease secretion inhibition performance detection
Test reagent:
fetal bovine serum, high-sugar DMEM medium, penicillin-streptomycin solution, trypsin-EDTA solution, modified oil red test kit, paraformaldehyde, isopropanol, isotretinoin, dihydrotestosterone (DHT).
Sample to be measured:
sample group: taking a certain amount of yeast fermentation product filtrate of the examples 1-5, and respectively diluting to 0.5v/v%, 1v/v% and 3v/v% by using physiological saline; blank control group: complete medium; negative control group: 1 μM DHT stimulation; positive control group: 1 μM DHT+5 μM isotretinoin.
Test protocol:
(1) Cell preparation: cells with good growth state were inoculated into 24-well plates, 500. Mu.l PBS solution was added to the edge wells of the plates, and a normal group, a positive control group and each sample group were set, respectively. Placement of 24-well plate in CO 2 Culturing overnight in an incubator.
(2) Administration: the medium in the 24-well plate was discarded, and the administration was performed. The normal group was given lml complete medium and the sample group was given 1ml of the corresponding concentration of the test sample. After dosing, 24-well plates were placed in CO 2 Culturing in an incubator.
(3) And (3) oil drop detection: after incubation, detection is carried out according to the instruction of the oil red detection kit, and the oil drop condition of each sample hole is detected by an inverted microscope and an enzyme-labeled instrument.
Determination criteria:
the drop content of the sample group was reduced compared to the negative control group under the same 1 μm DHT stimulation, and there was a significant difference (P < 0.05), indicating that the samples had the ability to inhibit lipid secretion at this tested concentration, and the results are shown in table 11.
TABLE 11 relative content of oil droplets in sebaceous gland cells and inhibition ratio
Note that: * Represents 0.01 < p <0.05, p < 0.01
Analysis of results:
compared with the negative control group, the oil drop contents of the examples 1-5 are obviously reduced, and the inhibition rates of 3v/v%, 1v/v% and 0.5v/v% of the example 3 are 31.835%, 23.022% and 12.641% respectively, which indicate that the saccharomycete fermentation product filtrate provided by the invention has the capacity of inhibiting the secretion of the sebaceous gland cell oil.
Finally, it should be noted that the above embodiments and comparative examples are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. The yeast fermentation product filtrate with the soothing and repairing effects is characterized by comprising the following components:
compounding a saccharomycete seed solution;
a liquid fermentation medium;
the inoculation amount of the compound saccharomycete seed liquid is 5-10v/v% of the liquid fermentation culture medium;
the liquid fermentation medium comprises the following components;
5-10g/L of soybean protein;
5-10g/L of modified wort;
10-30g/L of carbon source.
2. The yeast fermentation product filtrate with soothing and repairing effect according to claim 1, wherein the preparation method of the modified wort comprises the following steps: grinding malt, adding water, heating in boiling water for 20-40min, cooling to room temperature, adding glucose isomerase for reaction for 1-2 hr, heating to deactivate glucose isomerase, and filtering to obtain modified wort.
3. The yeast fermentation product filtrate with soothing and repairing effect according to claim 1, wherein the carbon source is at least one of sucrose, maltose, glucose, fructose, lactose, galactose, mannitol.
4. The yeast fermentation product filtrate with soothing and repairing effect according to claim 1, wherein the preparation method of the compound yeast seed solution comprises the following steps:
(1) Inoculating pichia pastoris in an activation culture medium, and placing the pichia pastoris in a shaking table at the temperature of 25-30 ℃ and the shaking culture speed of 250-300r/min for 24-48 hours;
(2) Inoculating the saccharomyces boulardii into an activation culture medium, and placing the culture medium into a shaking table for shake culture for 24-48 hours at the temperature of 28-30 ℃ and the speed of 180-220r/min, wherein the saccharomyces boulardii seed liquid;
(3) The pichia pastoris seed liquid and the Blakeslea pastoris seed liquid are compounded according to the volume ratio of (2-3): 1, and the compounded yeast seed liquid is obtained.
5. The yeast fermentation product filtrate with soothing and repairing effect according to claim 4, wherein the activating medium is added with: glucose 18-22g/L, yeast extract 9-11g/L, peptone 18-22g/L, wherein the mass ratio of glucose, yeast extract and peptone is (1.7-2.4): 1 (1.7-2.4).
6. The yeast fermentation product filtrate with soothing and repairing effect according to claim 5, wherein the mass ratio of glucose, yeast extract and peptone is 2:1:2.
7. A method for preparing a yeast fermentation product filtrate with soothing and repairing effects as claimed in any one of claims 1 to 6, characterized by comprising the following steps:
(1) Weighing soybean protein, modified wort and carbon source according to the formula amount to prepare a liquid fermentation culture medium;
(2) Inoculating the compound saccharomycete seed liquid with the formula amount into a liquid fermentation medium for fermentation to obtain fermentation liquor;
(3) Centrifuging, filtering and sterilizing the fermentation liquor obtained in the step (2) to obtain a saccharomycete fermentation product filtrate with a relieving and repairing effect.
8. The method for preparing a yeast fermentation product filtrate with soothing and repairing effect according to claim 7, wherein the fermentation culture conditions of the step (2) are as follows:
culturing for 0-24 h: the temperature is 28-30 ℃, the pH is 5.0-6.0, and the rpm is 180-200;
the culture time is 25-72 h: the temperature is 26-28deg.C, pH is 5.5-7.0, and 200-220rpm.
9. The method for preparing a filtrate of a yeast fermentation product with a soothing and repairing effect according to claim 7, wherein the centrifugation in the step (3) is carried out at 8000-10000rpm for 10-20min.
10. The method for preparing a yeast fermentation product filtrate with soothing and repairing effects according to claim 7, wherein the filtering and sterilizing in the step (3) is as follows: filtering and sterilizing by a polyether sulfone filter core with the diameter of less than or equal to 0.22 mu m.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518709A (en) * | 2020-05-06 | 2020-08-11 | 广州市巧美化妆品有限公司 | Saccharomyces cerevisiae strain YWY-1, fermentation filtrate prepared by using the strain, toning lotion prepared by using the filtrate and preparation method |
| CN115607483A (en) * | 2022-09-15 | 2023-01-17 | 广州市小谭科技有限公司 | Yeast fermentation composition with multiple effects and application thereof |
| CN115737490A (en) * | 2022-11-02 | 2023-03-07 | 稀物集(广州)生物科技有限公司 | Preparation method of Inonotus obliquus yeast fermentation liquor and application of Inonotus obliquus yeast fermentation liquor in cosmetics |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518709A (en) * | 2020-05-06 | 2020-08-11 | 广州市巧美化妆品有限公司 | Saccharomyces cerevisiae strain YWY-1, fermentation filtrate prepared by using the strain, toning lotion prepared by using the filtrate and preparation method |
| CN115607483A (en) * | 2022-09-15 | 2023-01-17 | 广州市小谭科技有限公司 | Yeast fermentation composition with multiple effects and application thereof |
| CN115737490A (en) * | 2022-11-02 | 2023-03-07 | 稀物集(广州)生物科技有限公司 | Preparation method of Inonotus obliquus yeast fermentation liquor and application of Inonotus obliquus yeast fermentation liquor in cosmetics |
Non-Patent Citations (3)
| Title |
|---|
| NILANJANA DAS等: "RECENT ADVANCES ON EFFICACY OF PROBIOTIC YEASTS IN HUMAN WELFARE: AN OVERVIEW", J MICROBIOL BIOTECH FOOD SCI, vol. 12, no. 3, pages 1 - 7 * |
| 杜晓蒙等: "培养基组成对布拉氏酵母生长的影响及其优化", 食品研究与开发, vol. 35, no. 5, pages 105 - 108 * |
| 鲍佳生等: "酵母生物活性物质及其化妆品功效研究进展", 生物技术进展, vol. 13, no. 3, pages 345 - 352 * |
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