CN117004758B - Molecular markers and primers related to broccoli and cauliflower ball development and application thereof - Google Patents

Molecular markers and primers related to broccoli and cauliflower ball development and application thereof Download PDF

Info

Publication number
CN117004758B
CN117004758B CN202311122986.5A CN202311122986A CN117004758B CN 117004758 B CN117004758 B CN 117004758B CN 202311122986 A CN202311122986 A CN 202311122986A CN 117004758 B CN117004758 B CN 117004758B
Authority
CN
China
Prior art keywords
broccoli
cauliflower
cabbage
plant
molecular marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202311122986.5A
Other languages
Chinese (zh)
Other versions
CN117004758A (en
Inventor
程锋
张扬勇
李幸
张磊
韩风庆
张亢
吕红豪
唐琪
王勇
季家磊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
Original Assignee
Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences filed Critical Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
Priority to CN202311122986.5A priority Critical patent/CN117004758B/en
Publication of CN117004758A publication Critical patent/CN117004758A/en
Application granted granted Critical
Publication of CN117004758B publication Critical patent/CN117004758B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses molecular markers, primers and application related to broccoli and cauliflower ball development. The invention is based on broccoli, cauliflower and other vegetables of the cabbage typeBoPNYThe structural variation site on the gene promoter develops a structural variation type molecular marker closely related to the development of broccoli and cauliflower heads, and whether the structural variation exists in the cabbage vegetables or not is detected through the molecular marker, so that whether the detection material is the broccoli and the cauliflower can be rapidly identified, and the molecular marker is further used for biotechnological auxiliary breeding.

Description

Molecular markers and primers related to broccoli and cauliflower ball development and application thereof
Technical Field
The invention relates to the field of molecular breeding, in particular to a molecular marker closely related to development of broccoli and cauliflower bulbs, a specific primer and application thereof.
Background
Cauliflower (Cauliflower)Brassica oleracea var. botrytis) Heqinghua vegetableBrassica oleraceavar. italica) Is a variety of cabbage belonging to Brassica genus of BrassicaceaeBrassicaL.) crops, the cauliflower is rich in nutrition, contains rich dietary fibers, multiple vitamins and minerals such as calcium, phosphorus, iron and the like, and has a health care function. The broccoli is called broccoli, the average nutritive value and disease prevention effect of the broccoli are far superior to those of other vegetables, the broccoli contains anticancer substances of glucosinolate, the broccoli can prevent the growth of early cancer cells, and the broccoli has special health care effect and is one of ten health foods recommended by the U.S. J. The flower ball is a nutrient storage organ of the cauliflower and the broccoli and also is an edible part, and the flower ball is planted at the top end of the short stem and is an important economic property of the cauliflower and the broccoli.
Research on the genetic relationship and genetic diversity of cauliflower and broccoli and related species is of great significance to resource evaluation and creation and new variety cultivation. The molecular marker typing technology has the advantages of no environmental influence, short test period, more selectable markers and capability of performing high-throughput sequencing analysis, and is widely used for research and application of genetic diversity and kindred relation analysis, new variety identification, seed purity and variety authenticity detection, molecular marker assisted breeding and the like.
For example, chinese patent application CN114231656a discloses an SSR core primer set for identifying purity of hybrid of cauliflower, and screening method and application thereof, the SSR core primer set is: boGMS0624, OI11G11, boGMS0941; the alternative core primer group is as follows: boE607, boE761, boGMS2431, boGMS0808, boGMS1530. The method determines the evaluation index of the core primer suitable for the purity identification of the hybrid seeds of the broccoli and the broccoli by DNA fingerprint analysis of 70 hybrid seeds of the broccoli and the broccoli, and determines a set of primers for the purity identification of the hybrid seeds of the broccoli.
At present, molecular markers which are easier to detect, accurate, stable and reliable for cauliflower and broccoli are still lacking.
Disclosure of Invention
In order to solve at least part of the technical problems in the prior art, the invention provides a molecular marker, a primer group and application for identifying broccoli and cauliflower, thereby providing a theoretical basis for molecular assisted breeding of the broccoli and the cauliflower. Specifically, the present invention includes the following.
In a first aspect of the invention, there is provided a method for identifying whether a plant comprises broccoli and/or cauliflower comprising detecting the presence or absence of a polypeptide located in the DNA of the plant to be identifiedBoPNYAnd (3) marking the structural variation molecules of the upstream promoter of the gene.
In certain embodiments, the method according to the invention for identifying whether a plant comprises broccoli and/or cauliflower, wherein the molecular marker has the sequence shown in SEQ ID No. 1.
In certain embodiments, a method for identifying whether a plant comprises broccoli and/or cauliflower according to the invention, wherein the method comprises the steps of:
(1) Extracting sample DNA from a plant sample to be identified;
(2) Amplifying nucleic acid containing the molecular marker from the sample DNA by PCR using a forward primer having a sequence shown as SEQ ID NO.2 and a reverse primer having a sequence shown as SEQ ID NO. 3; and
(3) The plants were identified by detecting the length of the sample PCR products by electrophoresis.
In a second aspect of the invention, there is provided a molecular marker closely related to broccoli and cauliflower ball development, the molecular marker being capable of being used to identify broccoli and cauliflower, wherein the molecular marker is a structural variant molecular marker located atBoPNYThe upstream promoter of the gene.
In certain embodiments, the molecular markers for identifying broccoli and cauliflower according to the invention, wherein the molecular markers have the sequence shown in SEQ ID No. 1.
In a third aspect of the invention, there is provided a primer combination for amplifying a molecular marker according to the invention.
In certain embodiments, a primer combination according to the present invention comprises a forward primer having the sequence shown in SEQ ID NO.2 and a reverse primer having the sequence shown in SEQ ID NO. 3.
In a fourth aspect of the invention, there is provided a kit for identifying broccoli and/or cauliflower comprising a primer combination according to the invention.
In a fifth aspect of the invention there is provided the use of a molecular marker, primer combination or kit according to the invention in a broccoli and/or cauliflower genetic analysis.
In certain embodiments, the use according to the invention, wherein the genetic analysis comprises genetic diversity analysis, germplasm identification or assisted breeding.
The invention is based on broccoli, cauliflower and other vegetables of the cabbage typeBoPNYStructural variation sites on promoters at 532bp upstream of genes develop an SV molecular marker closely related to development of broccoli and cauliflower, so that the broccoli and the cauliflower can be distinguished, and whether the detection materials are the broccoli and the cauliflower can be rapidly identified by detecting whether the SV molecular marker exists in the cabbage vegetables.
Drawings
FIG. 1 is a schematic representation of broccoli and cauliflower and other brassica vegetables.
FIG. 2 shows the distinguishing effect of broccoli and cauliflower and some other materials with SV.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in the present invention, it is understood that the upper and lower limits of the ranges and each intermediate value therebetween are specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
Unless otherwise indicated, the gene position in the present invention refers to a position relative to cabbage T10.
The invention relates to a broccoli and flower the broccoli refers to the cruciferae familyCruciferae) Brassica genusBrassicaL.) variants. The term "variant" means that a species is initially published on an academic journal without a grade under the species (i.e., variant, subspecies, variant), and later as one gets knowledge of the species, it is found that there are individuals or groups of plants within the species that have a variation from the characteristics of the species that were recognized when the species was initially published, and that the variation is obvious and stable, and it is worth separating them individually to show the difference, the plant scientist would name the type with the variation and publish it as a variant within the species according to circumstances. In contrast, a type that has the original characteristics and does not undergo significant variation is called the original variant of this variant.
In a first aspect of the invention, a specific molecular marker is provided, which has a characteristic sequence that distinguishes broccoli and/or cauliflower from other cabbage species, which is closely related to broccoli and cauliflower head development. The molecular marker is a structural variation with the length of 112bp, which is positioned inBoPNYA promoter region 532bp upstream of the gene. In a specific embodiment, the sequence has the sequence of SEQ ID NO1, a nucleotide sequence shown in the specification.
In the present invention, other brassica is intended to include any cruciferous species other than broccoli and cauliflower, preferably any brassica species, examples of which include, but are not limited to, common head cabbage, broccoli, cauliflower, cabbage mustard, kohlrabi, edible kale, brussels sprouts, and the like.
In a second aspect of the invention, there is provided a specific primer combination for amplifying a molecular marker of the invention, comprising a forward primer and a reverse primer. In a specific embodiment, the forward primer has the sequence shown in SEQ ID NO.2 and the reverse primer has the sequence shown in SEQ ID NO. 3.
The invention also provides a kit for identifying broccoli and/or cauliflower, comprising the primer combination of the invention and optionally reagents for polymerase chain reaction. Reagents for the polymerase chain reaction include any of those used in conventional PCR, such as a polymerase, a buffer, and the like.
In addition to the components described above, the kits of the present invention may also include precautions related to regulating manufacturing, use, or marketing of the diagnostic kit in a form prescribed by a government agency. In addition, the kits of the invention may also be provided with detailed instructions for use, storage and troubleshooting. The kit may also optionally be provided in a suitable device, preferably for robotic operation in a high throughput setting.
In certain embodiments, the components of the kits of the invention may be provided as dry powders. When the reagents and/or components are provided as dry powders, the powders may be restored by the addition of a suitable solvent. It is contemplated that the solvent may also be disposed in another container. The container will typically include at least one vial, test tube, flask, bottle, syringe, and/or other container means, with the solvent optionally being placed in aliquots. The kit may further comprise means for a second container comprising a sterile, pharmaceutically acceptable buffer and/or other solvent.
In certain embodiments, the components of the kits of the invention may be provided in solution, e.g., in aqueous solution. Where present in aqueous solution, the concentration or amount of these ingredients can be readily determined by one skilled in the art according to various needs. For example, for storage purposes, the concentration of the components may be present in a higher form, and the concentration may be reduced to the working concentration by, for example, diluting the higher concentration solution when in the working state or in use.
Where more than one component is present in a kit, the kit will also typically contain a second, third or other additional container in which additional components may be placed separately. In addition, combinations of various components may be included in the container. Any combination or reagent described herein may be a component in a kit.
The invention further provides the use of molecular markers, substances, primer combinations or kits capable of detecting said molecular markers in genetic analysis of broccoli and/or cauliflower, including but not limited to genetic diversity analysis, germplasm identification, assisted breeding, and the like. Substances capable of detecting the molecular markers include those polynucleotide molecules capable of complementary pairing with the target molecule, and the like.
The present invention provides a method for identifying whether a plant comprises broccoli and/or cauliflower. Preferably, the plant is from the crucifer familyCruciferae) More preferably, the plant described herein is from Brassica speciesBrassica) Further preferred, the plant described herein is a plant from the brassica oleracea class of vegetables. In certain embodiments, a "plant" herein is a type of plant, where the methods of the invention refer to methods for identifying whether the plant is broccoli and/or cauliflower. In certain embodiments, a "plant" herein is a mixture of multiple types of plants or plant components thereof, where the methods of the invention refer to methods for identifying whether broccoli and/or cauliflower are included in a plant.
The method of the invention comprises detecting the presence or absence of broccoli and/or cauliflower in the DNA of the plant to be identifiedBoPNYA step of gene characteristic sequence. The feature sequences have already been described and will not be described in detail here.
In the invention, whether the broccoli and/or the cauliflower are located is detectedBoPNYThe method of characterizing the gene may employ any method known in the art, examples of which include a method of amplifying a nucleic acid comprising the characterizing sequence, a method of directly sequencing a plant genome, and the like.
In certain embodiments, the methods of the invention for identifying whether a plant comprises broccoli and/or cauliflower comprise the step of amplifying a gene sequence comprising a signature sequence from DNA of a plant sample to be identified by PCR using forward and reverse primers.
In an exemplary embodiment, a method for identifying whether a plant comprises broccoli and/or cauliflower comprises the steps of:
(1) Extracting sample DNA from a plant sample to be identified;
(2) Amplifying nucleic acid containing the molecular marker from the sample DNA by PCR using forward primer and reverse primer;
(3) The length of the sample PCR product was checked by electrophoresis to identify whether the plant was broccoli and/or cauliflower.
In step (1), the "plant sample to be identified" refers to the whole plant or a part of the plant, e.g., the plant root, leaf or stem, etc.
In step (2), the ratio of the forward primer to the reverse primer is 1:1.PCR reaction procedure: 85-98deg.C for 1-5min, 85-98deg.C for 10-30s, 40-70deg.C for 10-30s, 50-80deg.C for 10-30s, and 10-40 cycles for 1-10 min. Also preferably, the PCR reaction procedure: 90-96 ℃ for 1-3min, 90-96 ℃,12-20s, 50-65 ℃,15-20s, 70-75 ℃ for 10-20s, 10-30 cycles, 65-75 ℃ for 1-5min.
In the step (3), the length of the PCR product of the sample is detected by electrophoresis, the amplified fragments of broccoli and cauliflower are 256bp, and the amplified fragments of other cabbage materials are 368bp.
Examples
The following shows a close relationship with broccoli and cauliflower ball developmentBoPNYDevelopment of molecular markers for Structural Variation (SV) of gene promoter and blue-and-whiteAnd (5) identifying vegetables and cauliflowers.
The SV-based patterned flood genome was constructed using high quality genomes of 27 different cabbage varieties, including 2 wild cabbage, 7 common head cabbage, 7 collard, 3 broccoli, 2 kohlrabi, 2 cabbage mustard, 1 brussels sprout, 1 ornamental cabbage, as follows.
With wild cabbage T10 as the reference genome, other genomes were aligned to T10 using NUCmer (v4.0.0) (Mar ç ais et al, 2018) with parameters set as: -maxmtch-c 100-l50, filtering the comparison results, using delta-filters with parameters: -m-i 90-l100, using show-records for the filtered result conversion format, the parameters being: -T-H-r-d.
SV was identified from the alignment using SyRI (Goel et al, 2019) and pooled for redundancy between different genomes.
The non-redundant SV was combined with the T10 reference genome using VG ToolKit (v1.33.0) (Garrison et al 2018) to construct a patterned flood genome based on SV.
Resequencing 704 parts of a different variety of cabbage material, comprising: 36 parts of wild cabbage, 310 parts of common head cabbage, 153 parts of broccoli, 63 parts of broccoli, 46 parts of kohlrabi, 34 parts of kale, 24 parts of cabbage mustard, 20 parts of brussels sprouts and 18 parts of ornamental cabbage, and the sequencing platform is Illumina HiSeq 2000, and the original data is filtered by using Trimmomatic v0.39 to remove joints, poly-N and low quality fragments, so that clean data is obtained. The VG Index is utilized to establish indexes in xg and gcsa formats for the SV-based graphical pan genome, then the VG Map is utilized to compare clean data to the graphical pan genome, and the VG Pack is utilized to filter the low-quality comparison result, wherein the parameters are as follows: -Q5. The SV genotype of each sample was obtained using VG Call with the parameters: -a-s.
Correlation analysis of SV with cabbage materials of different variety types revealed a characteristic SV closely related to broccoli and cauliflower head development, which was 112bp in length, absent in 151 parts broccoli and 61 broccoli, present in 2 parts broccoli and 2 parts broccoli, 386 parts (89%) in the other 434 parts cabbage materialThe SV was present and 48 parts of material was absent. The SV is located atBoPNYA promoter 532bp upstream of the gene.
Extracting genome DNA from the seedlings of broccoli, cauliflower and common head cabbage, and synthesizing SV molecular marker primers with the following sequences: boPNYSV-F: ATCTCTCTACCGATTACAGCCGG (SEQ ID NO. 2); boPNYSV-R: ACCAGCTATAGGTAGAGCTTAATCCAC (SEQ ID NO. 3).
And carrying out PCR reaction by taking the DNA of different types of cabbage materials as templates, and carrying out agarose gel electrophoresis detection on the obtained products, wherein the electrophoresis result is shown in figure 2, the obtained products of broccoli and cauliflower materials are 256bp, and the obtained product of the common head cabbage material is 368bp. Meanwhile, the product is subjected to sanger sequencing, and the sequencing result shows that the SV region sequence is as follows: TTTTTTTTTTTTTTATAAGTATGTGAATTTCATTGTCAAGATAATGACACTGGGGATACAAATAGTTTTTGCAAGCAAAACCTACTGCATCAATGCATCGCTTATATAAATC (SEQ ID NO. 1), 316bp in total.
By using the molecular marker primer, 50 parts of cabbage materials are identified, wherein the molecular marker primer comprises 10 parts of broccoli and 15 parts of broccoli materials, 25 parts of other cabbage materials, and the other cabbage materials comprise 10 parts of common head cabbage, 3 parts of cabbage mustard, 3 parts of kohlrabi, 3 parts of edible collard, 3 parts of ornamental collard and 3 parts of brussels sprouts. Extracting genome DNA of all materials, and performing PCR reaction identification.
The results show that: 25 parts of broccoli and cauliflower materials are 256bp, and the identification accuracy is 100%; of 25 parts of other cabbage materials, 24 parts are 365 bp,1 part of undetected bands, and the accuracy is 96%.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples are exemplary only.

Claims (1)

1. A method for identifying whether a plant is broccoli or cauliflower, characterized in that the presence or absence of a polypeptide located in the DNA of the plant to be identified is detectedBoPNY geneThe sequence of the molecular marker is shown as SEQ ID NO.1, and the method comprises the following steps:
(1) Extracting sample DNA from a plant sample to be identified;
(2) Amplifying nucleic acid containing the molecular marker from the sample DNA by PCR using a forward primer and a reverse primer, wherein the sequence of the forward primer is shown as SEQ ID NO.2, and the sequence of the reverse primer is shown as SEQ ID NO. 3; and
(3) The plants were identified by detecting the length of the sample PCR products by electrophoresis, and when the length of the obtained PCR products was 256bp, the plants were broccoli or cauliflower.
CN202311122986.5A 2023-09-01 2023-09-01 Molecular markers and primers related to broccoli and cauliflower ball development and application thereof Active CN117004758B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311122986.5A CN117004758B (en) 2023-09-01 2023-09-01 Molecular markers and primers related to broccoli and cauliflower ball development and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311122986.5A CN117004758B (en) 2023-09-01 2023-09-01 Molecular markers and primers related to broccoli and cauliflower ball development and application thereof

Publications (2)

Publication Number Publication Date
CN117004758A CN117004758A (en) 2023-11-07
CN117004758B true CN117004758B (en) 2024-03-22

Family

ID=88563793

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202311122986.5A Active CN117004758B (en) 2023-09-01 2023-09-01 Molecular markers and primers related to broccoli and cauliflower ball development and application thereof

Country Status (1)

Country Link
CN (1) CN117004758B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113355446A (en) * 2021-06-23 2021-09-07 中国农业科学院蔬菜花卉研究所 InDel molecular marker related to purple character of Chinese cabbage and application thereof
CN113528698A (en) * 2021-07-14 2021-10-22 中国农业科学院油料作物研究所 InDel molecular marker for identifying and/or distinguishing cabbage vegetables and application thereof
KR20220056806A (en) * 2020-10-28 2022-05-06 서울대학교산학협력단 DNA marker for discriminating genotype of Brassica rapa, Brassica oleracea and their interspecific hybrid and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220056806A (en) * 2020-10-28 2022-05-06 서울대학교산학협력단 DNA marker for discriminating genotype of Brassica rapa, Brassica oleracea and their interspecific hybrid and uses thereof
CN113355446A (en) * 2021-06-23 2021-09-07 中国农业科学院蔬菜花卉研究所 InDel molecular marker related to purple character of Chinese cabbage and application thereof
CN113528698A (en) * 2021-07-14 2021-10-22 中国农业科学院油料作物研究所 InDel molecular marker for identifying and/or distinguishing cabbage vegetables and application thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GenBank：HG994366.1.GenBank：HG994366.1.GenBank.2021,第1页. *
GenBank：HG994366.1;GenBank：HG994366.1;GenBank;第1页 *
GenBank：LR031874.1.GenBank：LR031874.1.GenBank.2018,第1页. *
GenBank：LR031874.1;GenBank：LR031874.1;GenBank;第1页 *
Genome sequencing sheds light on the contribution of structural variants to Brassica oleracea diversification;Guo N等;BMC Biol;第1-15页 *

Also Published As

Publication number Publication date
CN117004758A (en) 2023-11-07

Similar Documents

Publication Publication Date Title
Sargent et al. An enhanced microsatellite map of diploid Fragaria
CN110016509B (en) SCAR marker for potato ladybug and eggplant coccinella twenty-eight and application thereof
CN111733281B (en) Molecular marker for identifying peroxidase activity of wheat grains and application thereof
CN111961750A (en) KASP primer for detecting tomato yellow leaf curl virus disease resistance gene Ty-1 and application thereof
CN104789654A (en) Molecular marker for rice blast resistance gene Pita and application thereof
CN109295179B (en) Method for screening wheat with different zinc content and iron content and special kit thereof
CN111424111B (en) Method for identifying radish clubroot disease resistance
CN112746124B (en) SSR primer and method for purity identification of cauliflower hybrid seeds
CN112143829B (en) Typing and application of universal SNP (single nucleotide polymorphism) markers
CN117004758B (en) Molecular markers and primers related to broccoli and cauliflower ball development and application thereof
CN112725518A (en) PARMS marker based on SNP mutation of coding region of rice blast resistance gene Pid2 and application
CN117363768B (en) Molecular marker and primer for inhibiting development of broccoli and cauliflower leaves and application of molecular marker and primer
CN111850155A (en) Application of specific target primer in simultaneous and rapid identification of two pathogenic bacteria of strawberry infection
CN117248052B (en) Molecular marker and primer group related to ornamental collard color and application
CN107988418B (en) Primer group, kit and method for pure heterozygous identification of transgenic papaya YK16-0-1 transformant
CN117431332B (en) Molecular marker of common head cabbage leaf bulb development gene BoKAN and application
CN117210602B (en) Molecular marker of common head cabbage leaf bulb development gene BoACS4 and application
CN113265483B (en) Method for rapidly identifying 4St chromosome of Elytrigia intermedium
CN113278723B (en) Composition for analyzing genetic diversity of Chinese cabbage genome segment or genetic diversity introduced in synthetic mustard and application
CN112779274B (en) Ribosomal RNA gene of mulberry plaster disease pathogenic bacteria and application thereof
CN112410458A (en) PCR primer for quickly and early identifying weedy rice and application thereof
KR101301867B1 (en) Microsatellite primer set for identifying onion varieties
CN110218813A (en) Detect molecular labeling, primer, detection method and the application of maize aphids resistant gene
CN112852982B (en) Product for classifying cadmium-resistant vegetable varieties
KR102273447B1 (en) A set of SNP molecular markers for discriminating radish accessions using KASP assay and uses thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant