CN116908432B - 一种基于光切割的荧光分析检测黄曲霉毒素b1含量的方法 - Google Patents
一种基于光切割的荧光分析检测黄曲霉毒素b1含量的方法 Download PDFInfo
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- CN116908432B CN116908432B CN202310835039.4A CN202310835039A CN116908432B CN 116908432 B CN116908432 B CN 116908432B CN 202310835039 A CN202310835039 A CN 202310835039A CN 116908432 B CN116908432 B CN 116908432B
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- 229930020125 aflatoxin-B1 Natural products 0.000 title claims abstract description 114
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- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 description 8
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- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
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- JGBUYEVOKHLFID-UHFFFAOYSA-N gelred Chemical compound [I-].[I-].C=1C(N)=CC=C(C2=CC=C(N)C=C2[N+]=2CCCCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCC[N+]=3C4=CC(N)=CC=C4C4=CC=C(N)C=C4C=3C=3C=CC=CC=3)C=1C=2C1=CC=CC=C1 JGBUYEVOKHLFID-UHFFFAOYSA-N 0.000 description 1
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- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/14—Macromolecular compounds
- C09K2211/1408—Carbocyclic compounds
- C09K2211/1425—Non-condensed systems
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- C09K2211/1441—Heterocyclic
- C09K2211/145—Heterocyclic containing oxygen as the only heteroatom
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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CN107129989A (zh) * | 2017-05-17 | 2017-09-05 | 中国科学院生态环境研究中心 | 检测黄曲霉毒素的核酸适配体、试剂盒及其检测方法 |
CN109406475A (zh) * | 2018-12-13 | 2019-03-01 | 四川大学 | 双标记快速响应核酸适配体探针及其检测黄曲霉毒素b1的方法 |
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JP2012105644A (ja) * | 2010-10-28 | 2012-06-07 | Arkray Inc | 多型検出用プローブ、多型検出方法、薬効評価方法、および多型検出用キット |
CN109490264B (zh) * | 2018-11-02 | 2020-06-23 | 四川大学 | 基于聚集发光的双端互补核酸适配体探针及黄曲霉毒素b1均相免标记检测方法 |
CN109946274B (zh) * | 2019-03-15 | 2021-04-09 | 四川大学 | 一种基于适配体固有构象诱导的检测黄曲霉毒素b1的方法 |
CN112574998B (zh) * | 2021-01-05 | 2022-10-14 | 江苏省原子医学研究所 | 检测黄曲霉毒素b1的探针组、试剂盒及其应用 |
CN112816450B (zh) * | 2021-01-07 | 2022-12-06 | 青岛农业大学 | 检测黄曲霉毒素b1的试剂盒及检测黄曲霉毒素b1的方法 |
CN114807147B (zh) * | 2021-01-19 | 2023-08-15 | 中国科学院苏州纳米技术与纳米仿生研究所 | 黄曲霉毒素b1的核酸适配体及其应用 |
CN113897458B (zh) * | 2021-06-02 | 2024-01-16 | 浙江省淡水水产研究所 | 检测淡水鱼类桑提库珀蛙病毒的特异性引物、探针及快速检测试剂盒 |
CN113322256B (zh) * | 2021-06-22 | 2023-06-30 | 江苏省原子医学研究所 | 一种探针组、传感器、检测方法及其用途 |
CN116121345A (zh) * | 2023-01-12 | 2023-05-16 | 合肥工业大学 | 一种黄曲霉毒素b1荧光适体传感器及检测方法和试剂盒 |
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CN107129989A (zh) * | 2017-05-17 | 2017-09-05 | 中国科学院生态环境研究中心 | 检测黄曲霉毒素的核酸适配体、试剂盒及其检测方法 |
CN109406475A (zh) * | 2018-12-13 | 2019-03-01 | 四川大学 | 双标记快速响应核酸适配体探针及其检测黄曲霉毒素b1的方法 |
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