CN116875501A - Kitchen waste decomposing degradation microbial inoculum and preparation method and application thereof - Google Patents
Kitchen waste decomposing degradation microbial inoculum and preparation method and application thereof Download PDFInfo
- Publication number
- CN116875501A CN116875501A CN202310846392.2A CN202310846392A CN116875501A CN 116875501 A CN116875501 A CN 116875501A CN 202310846392 A CN202310846392 A CN 202310846392A CN 116875501 A CN116875501 A CN 116875501A
- Authority
- CN
- China
- Prior art keywords
- kitchen waste
- bacillus
- decomposing
- degradation
- degrading
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010806 kitchen waste Substances 0.000 title claims abstract description 43
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 13
- 230000015556 catabolic process Effects 0.000 title claims description 35
- 238000006731 degradation reaction Methods 0.000 title claims description 35
- 238000002360 preparation method Methods 0.000 title claims description 9
- 230000001580 bacterial effect Effects 0.000 claims abstract description 32
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 27
- 230000000593 degrading effect Effects 0.000 claims abstract description 24
- 238000004321 preservation Methods 0.000 claims abstract description 19
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 18
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 18
- 244000005700 microbiome Species 0.000 claims abstract description 13
- 238000009629 microbiological culture Methods 0.000 claims abstract description 10
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 7
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 36
- 239000010902 straw Substances 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 10
- 239000010813 municipal solid waste Substances 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000010564 aerobic fermentation Methods 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 abstract description 15
- 229920002678 cellulose Polymers 0.000 abstract description 15
- 239000002361 compost Substances 0.000 abstract description 4
- 239000004519 grease Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 24
- 108090000790 Enzymes Proteins 0.000 description 13
- 102000004190 Enzymes Human genes 0.000 description 13
- 229940088598 enzyme Drugs 0.000 description 13
- 239000001963 growth medium Substances 0.000 description 13
- 239000000725 suspension Substances 0.000 description 11
- 238000009264 composting Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 238000012258 culturing Methods 0.000 description 7
- 239000002054 inoculum Substances 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 108020004465 16S ribosomal RNA Proteins 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 108010084185 Cellulases Proteins 0.000 description 3
- 102000005575 Cellulases Human genes 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 3
- 108010047754 beta-Glucosidase Proteins 0.000 description 3
- 102000006995 beta-Glucosidase Human genes 0.000 description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 229910017053 inorganic salt Inorganic materials 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 3
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 3
- 241001494479 Pecora Species 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 101710112457 Exoglucanase Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- 241000850383 Methanobacterium alcaliphilum Species 0.000 description 1
- 102000002568 Multienzyme Complexes Human genes 0.000 description 1
- 108010093369 Multienzyme Complexes Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 241001262956 bacterium Y3 Species 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229930001119 polyketide Natural products 0.000 description 1
- 125000000830 polyketide group Chemical group 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention belongs to the field of microorganisms, and relates to a mixed bacterial agent for decomposing and degrading kitchen waste and application thereof, wherein the bacterial agent comprises bacillus subtilis Y1 (Bacillus subtilis) and bacillus bailii Y3 (Bacillus velezensis), the bacillus subtilis Y1 is preserved in China general microbiological culture Collection center with the preservation date of 2022 and 28 days and the preservation number of CGMCC NO.24449; the bacillus bailii Y3 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022, 2 and 28 days and a preservation number of CGMCC NO.24450. The microbial inoculum disclosed by the invention can degrade cellulose, grease and other refractory organic matters in kitchen waste compost, and promote the kitchen waste to be quickly decomposed.
Description
Technical Field
The invention belongs to the field of microorganisms, and relates to a kitchen waste decomposing and degrading microbial agent, and a preparation method and application thereof.
Background
Kitchen waste refers to a collective term of leftovers discarded by families, restaurants and various catering institutions. At present, the kitchen garbage is mainly treated by incineration, landfill, crushing and direct discharge and fertilizer production. Wherein, fertilizer preparation is one of the main modes of kitchen waste resource utilization. Aerobic composting is a main method for treating kitchen waste by fertilizer. Because kitchen garbage contains a large amount of cellulose, the cellulose has a complex molecular structure, is difficult to degrade naturally, and limits the composting period. Researches show that the period of composting and maturing can be effectively shortened by adding cellulose degrading bacteria into the compost. Li and the like can shorten the composting time by 3-5 days by adding cellulose degrading bacteria into the corn straw. Wu Qingshan and the like screen cellulose degrading bacteria from sheep manure, and the cellulose degrading bacteria are added into the sheep manure, so that the composting period is shortened. However, at present, degradation bacteria capable of efficiently degrading cellulose, grease and other hard-to-organic matters in kitchen waste are fewer.
Disclosure of Invention
The invention aims to provide a kitchen waste decomposing degradation microbial inoculum and application thereof, which can degrade cellulose, grease and other difficult organic matters in kitchen waste compost, promote the kitchen waste to decompose rapidly and provide technical support and theoretical basis for the resource utilization of kitchen waste.
The technical scheme provided by the invention is as follows:
the kitchen garbage decomposing degradation microbial agent comprises bacillus subtilis Y1 (Bacillus subtilis) and bacillus belicus Y3 (Bacillus velezensis), wherein the bacillus subtilis Y1 is preserved in the China general microbiological culture collection center (China general microbiological culture collection center) with a preservation date of 2022, 2 and 28 days and a preservation number of CGMCC NO.24449; the bacillus bailii Y3 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022, 2 and 28 days and a preservation number of CGMCC NO.24450.
Further, the colony number ratio of the bacillus subtilis Y1 to the bacillus belicus Y3 is 2:1.
The invention also provides a preparation method of the kitchen waste decomposing and degrading microbial inoculum, wherein the bacillus subtilis Y1 and the bacillus beijerinckii Y3 are mixed under the conditions that the inoculation amount (V/W is the mass ratio of mixed bacterial liquid to kitchen waste) is 10-20%, the rotating speed is 120-160 r/min, the pH is 7-8 and the fermentation temperature is 30 ℃ according to the proportion of Y1:Y3=2:1, so that the kitchen waste decomposing and degrading microbial inoculum is obtained.
Further, the inoculum size was 13.5%, the rotational speed was 132r/min, and the pH was 7.6.
The invention also provides a kitchen waste decomposing and degrading method, which comprises the steps of mixing the kitchen waste decomposing and degrading microbial inoculum of claim 1 with kitchen waste and carrying out aerobic fermentation.
Further, the bacterial load is 200g/t, and the rotating speed is 12r/min.
Further, the initial temperature was 60 ℃.
Further, the fermentation time was 16h.
The invention also provides a kitchen garbage decomposing degradation bacterium, which is classified and named as bacillus subtilis (Bacillus subtilis) and is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022 and a preservation number of CGMCC NO.24449 for 2 months and 28 days.
The invention also provides a kitchen garbage decomposing degradation bacterium, which is classified and named as bacillus bailii (Bacillus velezensis) and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022 and a preservation number of CGMCC NO.24450, wherein the preservation date is 2 and 28.
Advantageous effects
New mixed thalli with kitchen waste decomposing and degrading capacity are screened from the decomposed kitchen waste organic fertilizer, the degradation rate of the thalli on straw can reach 47.24% within ten days, and filter paper can be decomposed into paste within ten days. Compared with the prior art, the degradation period is shortened by 3-5 days. The treated granular powder has fermenting fragrance, and the total amount of fecal coliform groups in the output is less than or equal to 30cfu/g, which accords with the NY/T525-2021 standard of organic materials. The comprehensive energy consumption of each kilogram of kitchen waste treatment is less than or equal to 0.25℃, and the treatment cost per ton is about 80-150 yuan according to the seasons, the organic waste composition and the water content of the output, which is far lower than the energy consumption of the same kitchen waste treatment equipment.
Drawings
FIG. 1 is the ratio (H/D) of colony diameter (H) to transparent circle diameter (D) of strain Y1 cultured on sodium carboxymethylcellulose medium for three days;
FIG. 2 is the ratio (H/D) of colony diameter (H) to transparent circle diameter (D) of strain Y3 cultured on sodium carboxymethylcellulose medium for three days;
FIG. 3 is a graph showing the effect of strain Y1 on filter paper degradation over ten days;
FIG. 4 is a graph showing the effect of strain Y3 on filter paper degradation over ten days;
FIG. 5 is a diagram showing three cellulase activities of cellulose degrading bacteria Y1;
FIG. 6 is a chart showing the activities of three cellulases of cellulose degrading bacterium Y3;
FIG. 7 phylogenetic tree of the 16S rDNA full sequence of strain Y1;
FIG. 8 phylogenetic tree of the 16S rDNA full sequence of the Y3 strain;
FIG. 9 effect of different ratios on total yield of bacteria and ratio of bacteria;
FIG. 10 effects of different inoculum size on total yield of bacteria and bacterial ratio;
FIG. 11 effects of different pH values on overall bacterial yield and bacterial ratio;
FIG. 12 effects of different rotational speeds on overall yield of bacteria and bacterial ratios;
FIG. 13 effects of different temperatures on overall yield of bacteria and bacterial ratios.
Detailed Description
1. Screening methods of cellulose degrading strains Y1 and Y3.
Material preparation
1. Composting samples: the sample is obtained from organic fertilizer obtained by high temperature fermentation of Aolong environmental protection Co., suzhou, and the retrieved sample is stored in a refrigerator at 4 ℃ for standby.
2. Rice straw: is taken from Yongfeng farm in Taicang city. Cleaning the retrieved rice straw with clear water, placing in a 65 ℃ oven for baking to constant weight, cutting one part into 2cm sections, crushing the other part, sieving the crushed straw powder with a 100-mesh sieve, and preserving for later use.
3. Culture medium:
inorganic salt culture solution of Herzison's disease KH 2 PO 4 0.5g,NaCl 0.05g,MgSO 4 ·7H 2 O 0.15g,NaNO 3 1.25g,FeCl 3 0.005g,CaCl 2 0.05g, 500mL of distilled water, pH 7.2;
straw flat plate powder culture medium: 9g of agar powder, 5g of wheat straw powder and 500ml of Herzison inorganic salt culture solution;
straw enzyme production culture medium: 10g of rice straw powder and 500ml of Herzison inorganic salt culture solution.
Filter paper disintegration medium: three strips of 1cm by 6cm filter paper, KH 2 PO 4 0.1g，MgSO 4 ·7H 2 O 0.04g，(NH 4 ) 2 SO 4 0.3g, 0.01g of yeast powder and 100ml of distilled water.
LB medium: tryptone 5g, naCl 5g, yeast extract 2.5g, distilled water 500ml, pH 7.2.
The above culture media were all sterilized at 120℃for 30min.
Screening, isolation and purification of the second Strain
Placing 10g of low-temperature preserved compost in 90ml of sterile water, shaking in a constant-temperature shaking box (30deg.C, 200 r/min) for 30min, taking out, standing for 10min, collecting suspension 1ml, diluting with sterile water to obtain a suspension of 10 -1 、10 -2 、10 -3 、10 -4 And 10 -5 Five gradient concentrations, 100. Mu.l 10 were aspirated -5 The gradient diluent is coated in a straw flat-plate culture medium which takes straw as the sole carbon source. Culturing the culture medium in a constant temperature incubator at 30 ℃ for five days, selecting five strains with larger colony diameters for separation and purification, and purifying single colonies by a streaking method. Coating the purified strain on a slant culture medium, culturing in a constant temperature incubator at 30 ℃ for one day, taking out, and storing in a refrigerator at 4 ℃ for standby.
The purified five strains are respectively inoculated on a sodium carboxymethyl cellulose culture medium, placed in a constant temperature incubator (30 ℃) for culturing for five days, dyed with 1g/L Congo red solution for 20min, then the dye liquor is discarded, and washed with 1mol/L sodium chloride solution for 30min, and the strains with stronger cellulase production capacity, namely cellulose degradation strains Y1 and Y3, are primarily screened according to the ratio of the diameter (H) of a transparent ring to the diameter (D) of a colony, wherein the H/D ratio of the strains reaches 4.4 and 3.8 respectively, as shown in figures 1 and 2.
Colony morphology of two strains is similar: approximately round, white, opaque, flatter edge, smooth surface, convex middle and easy picking. Can grow at 15-45 ℃ and pH 5.0-10.0, and the most easy growth pH is 7. The growth is rapid, and the separation culture is easy. Can secrete and produce various bioactive substances. Including enzymes, antibacterial proteins, lipopeptides antibiotics, polyketides antibiotics, plant hormones, and the like.
(III) straw degradation experiment
Preparing bacterial suspension from the strain which is screened to produce the cellulase with stronger strength, transferring 10ml of bacterial suspension into a straw degradation culture medium, culturing in a constant temperature shake incubator (30 ℃ and 160 r/min) for twelve days, and taking out the straw. Repeatedly washing with distilled water for five times, and baking at 80deg.C to constant weight, wherein each treatment is performed in three times. The degradation rate of the straw is calculated according to a weight reduction method.
Straw degradation rate (%) = [ original straw mass (g) -dried straw mass (g) ]/original straw mass (g) ×100%
According to calculation, in the continuous twelve-day straw degradation experiment, the degradation rate of the straw in the straw degradation culture medium inoculated with the strain Y1 reaches 42.50%, and the degradation rate of the straw in the straw degradation culture medium inoculated with the strain Y3 reaches 40.94%.
(IV) Filter paper degradation experiment
Preparing bacterial suspension from the strain obtained by screening, respectively transferring 5ml of bacterial suspension into filter paper strip disintegration culture medium, placing into a constant temperature incubator (30 ℃ C., 160 r/min) for culturing for ten days, taking culture solution without strain inoculated as blank control, and observing the disintegration condition of filter paper. As shown in FIGS. 3 and 4, the results showed that the filter paper was decomposed to be approximately pasty in the medium added to the strain, while the filter paper was not substantially changed in the medium without the strain inoculated. The strain has strong degradation effect on filter paper.
Fifth, kitchen garbage decomposition and degradation experiment
Strains Y1 and Y3 were prepared as bacterial suspensions, and the bacterial suspensions were thoroughly mixed with 10kg of kitchen waste at inoculum sizes of 1%, 2%, 3%, 4% and 5%, respectively. Taking kitchen garbage without bacterial liquid as blank reference. Sampling every two days, measuring the C/N of the sample, and judging the influence of different treatments on the composting degree.
Experimental results show that the kitchen waste composting time of the inoculated strain is obviously less than that of a blank control, wherein the kitchen waste composting time with the inoculation amount of 3% is shortest and is 15 days. The decomposing time of the kitchen waste without inoculating the bacterial suspension is up to 45 days, which indicates that the bacterial strains can greatly shorten the decomposing time of the kitchen waste.
2. Measurement of cellulase Activity
Preparation of crude enzyme solution
Preparing bacterial suspension from bacterial strains Y1 and Y3 respectively, transferring 10ml of bacterial suspension into liquid enzyme production culture solution, continuously culturing in a constant temperature incubator (30 ℃ C., 120 r/min) for 15 days, taking 8-10ml of culture solution every 24h, filtering the taken culture solution with a water system filter head of 0.45um, and obtaining filtrate, namely crude enzyme solution.
(II) measurement of enzyme Activity
Cellulases are multienzyme complexes comprising three major components, i.e., endo-and exo-glucanases and beta-glucosidase. The enzyme activity is defined as the amount of enzyme per minute required to catalyze the production of substrate equivalent to 1ug of p-nitrophenol in 1mL of stock enzyme solution, which is one enzyme activity unit (U/mL). By the strain cellulase activity experiments for fifteen consecutive days, as shown in fig. 5 and 6, it was found that the three cellulase activity peaks of the strains Y1 and Y3 all appeared on the sixth day, which suggests that the three cellulases of the strains Y1 and Y3 have a strong synergistic effect. Meanwhile, the two have very similar physicochemical properties, and can be used as digestion bacteria simultaneously. The peaks of the endo-and exo-and beta-glucosidase activities of Y1 were 25.63, 21.54, 18.41. Mu. Mol/ml, respectively. The peaks of the endo-glucose enzyme, the exo-glucose enzyme and the beta-glucosidase activity of Y3 are 20.53 and 23.65,8.94U/ml respectively.
3. Identification of cellulose degrading strains
The morphological identification of the strains was performed by reference to the "Berger's Manual of identification of strains (8 th edition)," manual of identification of common bacterial systems ", and gram staining.
Bacterial total DNA was extracted with forward primer 27F:5'-AGAGTTTGATCCTGGCTCAG-3', the reverse primer is 1499R:5 '-GGTTACCT TGTTAC GACTT-3'. The PCR reaction system is 20 mu L, and the reaction conditions are as follows: pre-denaturation at 95 ℃ for 5min; denaturation at 95℃for 30s, annealing at 55℃for 30s, extension at 72℃for 45s for 28 cycles. The PCR product was purified and sent to Shanghai Ling En Biotechnology Co.Ltd for sequencing.
The DNA sequences of the sequenced strains were subjected to Blast sequence comparison analysis in NCBI. The MEGA7.0 strain 16S rDNA phylogenetic tree was constructed.
4. The strain is a new strain
And submitting the 16S rDNA sequence obtained by sequencing the strain to NCBI for Blast comparison, and constructing a phylogenetic tree of the two strains by using an adjacent method in MEGA7.O software. The tree was constructed using M.alcaligenes (Methanobacterium alcaliphilum, AB 496639.1) as the exoid and selection of other closely related species was determined by Blast and NCBI Taxonomy database. From the perspective of the evolutionary tree, both Y1 and Y3 belong to bacillus, and Y1 and bacillus subtilis (Bacillus subtilis YEBN, MT 372156.1) have the closest evolutionary distance and are gathered in the same branch, so that the strain Y1 belongs to bacillus subtilis, as shown in FIG. 7. Y3 and Bacillus belicus (Bacillus velezensis strain FZB, NR 075005.2) have the closest evolutionary distance, are gathered in the same branch, and the Y3 strain is determined to be Bacillus belicus (Bacillus velezensis) by combining the morphological characteristics of the Y3 strain, as shown in FIG. 8.
5. Optimization of strain mixing ratio
Antagonism test (one)
The single Y1 and Y3 colonies are respectively picked from the flat plate by using a fungus inoculation ring, inoculated on the same flat plate by a well-shaped drawing method, placed in a constant temperature incubator for 2 days for culturing, and then the growth condition of the colonies on the flat plate is observed. Bacteria at the crossing point of the two bacteria grow normally, and the two strains have no antagonism and can be co-cultured.
(two) one-factor experiment
(1) Effect of the ratio of inoculation on the yield and ratio of two Bacillus species
When two kinds of bacillus are mixed for fermentation, a competition relationship exists between the two kinds of bacillus, and the proportion of the initial inoculation quantity of the two kinds of bacillus is controlled well, so that the balanced growth of the two kinds of bacillus is facilitated. As can be seen from fig. 9, when the ratio of the two initial bacteria is y1:y3=2:1, the total amount of bacteria is maximum, and the number of bacteria reaches a peak value of 4.36×10 6 cfu/mL。
(2) Effect of inoculum size on yield and ratio of two Bacillus species
The bacterial inoculation amount influences the growth of bacterial colonies, so that the control of the bacterial inoculation amount is important for the mixed culture of two strains. As shown in fig. 10, the number of bacteria tended to increase and decrease with increasing inoculum size, and when the inoculum size was 10% and 20%, the ratio of the numbers of two types of bacillus was large, the number of bacteria was large, and the difference was significant compared with 1% and 50%. Therefore, when the strains Y1 and Y3 are mixed, the inoculation amount is 10-20% which is the optimal growth condition.
(3) Influence of pH on yield and ratio of two Bacillus species
Microorganisms regulate cell permeability by acid-base, pH affects intracellular protein structure and function of cells, and as shown in fig. 11, the ratio of two bacteria reaches a maximum of 0.48 at pH 9.0, but the number is minimal, and the difference is significant compared to the other three groups. At initial pH 7.0 and 8.0, both bacillus strains grew vigorously, in greater numbers, and were significantly different from the other two groups. Therefore, when fermenting the mixed strains Y1 and Y3, the initial pH value is 7.0 to 8.0 as the optimum growth condition.
(4) Influence of the rotational speed on the yield and the ratio of the two Bacillus species
The two strains Y1 and Y3 of the mixed culture in the experiment are aerobic microorganisms, and the rotation speed has obvious influence on the dissolved oxygen amount in the culture medium, so that the control of the rotation speed has important significance for the mixed culture. As shown in fig. 12, the number of bacteria increases and then decreases with increasing rotation speed, but the difference in number is not significant. The ratio of the two bacteria was maximum at speeds of 120r/min and 160r/min, with a clear difference compared to the other groups. Therefore, when the strains Y1 and Y3 are mixed, the rotation speed is 120-160 r/min, and the optimal growth condition is achieved.
(5) Influence of temperature on yield and ratio of two Bacillus species
The temperature not only affects the activity of enzymes in the microorganism, but also affects the expression of proteins in the cells of the microorganism. At a temperature of 30 ℃, the number of bacteria and the ratio of both bacteria reached a maximum and the differences compared to the other groups were significant. At this time, the number of cells reached a peak of 6.6X10 6 cfu/ml, the ratio of the two bacillus numbers is 0.52. Therefore, the optimal culture temperature at the time of mixing the fermentation strains Y1 and Y3 was 30 ℃.
(6) Optimizing culture conditions through response surface
And obtaining the optimal culture condition by solving an inverse matrix of a quadratic polynomial mathematical model of the thallus concentration according to the experimental result of the response surface: the inoculum size was 13.5%, the rotational speed was 132r/min, the pH was 7.6, the optimal initial ratio was Y1:Y3=2:1, and the fermentation temperature was 30 ℃.
(7) Straw degradation experiment is carried out on the mixed bacterial liquid
Table 1 comparison of degradation effects of mixed bacteria
Compared with the single strain, the mixed strain has higher straw degradation rate, better filter paper disintegration effect and better cellulose degradation capability.
6. Mixed microbial inoculum process application optimization
The mixed bacteria are prepared into water-dispersed mixed bacteria agent (the bacterial content is 2.52 multiplied by 10) 8 cfu/g), and the microbial inoculum is applied to kitchen waste treatment equipment with daily treatment capacity of 5t, and simultaneously, the running process of the kitchen waste treatment equipment is optimized by adopting a four-factor three-level orthogonal test design. Four factors are bacterial dose, duration, rotational speed and onset temperature, three levels were set for each factor, see in particular table 2.
TABLE 2 orthogonal test factor level Condition Table
TABLE 3 orthogonal test design table
TABLE 4 Quadrature test error analysis
As shown in the results of the orthogonal test (Table 4), as the amount of the added bacteria increased, the C/N value increased and then decreased, and the maximum amount of the added bacteria was reached at 200 g/t. The fermentation time is between 0 and 16 hours, the C/N value is higher and higher, and the C/N value reaches the maximum when 16 hours are reached, and then the C/N value starts to decrease. The C/N value continues to decrease at a speed of 12-15r/min, reaches a minimum at 15r/min, and then starts to rise slowly, but the overall process is still lower than the C/N value at a speed of 12r/min. The C/N value increases with increasing temperature at 50-60℃and increases at maximum when the temperature reaches 60℃and then begins to decrease slowly. According to the experimental result, when the bacterial load is 200g/t, the reaction time is 16h, the rotating speed is 12r/min, the initial temperature is 60 ℃, the condition of one-time aerobic fermentation is best, and the kitchen waste can be rapidly fermented under the condition.
Splice sequence of Strain Y1 (SEQ ID NO. 1)
GCAGGGCGGCAGCTATACATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTATTTCGGATCGTAAAGCTTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCCGGGGGCAGAGTGACAGGTGGTGCATGGGTTGTCGTCAGCTTCGTGTCGATGAGATGTTTGGGTTAAGTCCCGCAACGAGCCGCCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAGGTGACAAGAGG；
Splice sequence of Strain Y3 (SEQ ID NO. 2)
CGGCAGTGCGGGGAGCTATAATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTAGTATGACAGGATTATTCGGATCGCTAAAAGCTCTTGTTGTTTAGGGAAAGAACAAGATGCCGTTCAAATGAGGGCCGGCACCGTTGACGGTACCTAACCAGAAAAGCCAACGGCTAACTACCGTGCCAGCAGCCGCGGTTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAAGGGCTCGCAGGGCGGTTTCTTAAAGTCTGATGTGAA
AGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGGAACTTGAG
TGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAAATGCGTAGAG
ATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACG
CTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTC
CACGCCGTAAACGATGAGTGCTAAAGTGTTAGGGGGTTTCCGCCCCCTTAG
TGCTGCAGCTAACGCATTAAGCCACTCCGCCCTGGGGAGTACTGGTCGCA
AGAACTGATAACTCAAAGGAATTGACGGGGGCCCCGCACAAGCGGTGGA
AGCATGTGGTTTGAATATCGAAGCAACGCGAAGAACCTTACACAGGTCTT
GATCATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGT
GACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAA
GTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGC
ACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGT
CAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACA
GAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTT
CTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCT
AGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACA
CACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTA
ACCTTTTAGGAGCCAGCCGCCGAAGTTGACAAGAGGG。
Claims (10)
1. A kitchen garbage decomposing degradation bacterium is characterized in that the kitchen garbage decomposing degradation bacterium is classified and named as bacillus bailii (Bacillus velezensis) Y3 and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022 and a preservation number of CGMCC NO.24450 for 28 months.
2. The application of the kitchen waste decomposing and degrading bacterium in straw degradation.
3. The use of the kitchen waste decomposing and degrading bacterium in filter paper degradation.
4. The use of the kitchen waste decomposing and degrading bacterium in accordance with claim 1 in cellulase production.
5. The kitchen garbage decomposing and degrading microbial agent is characterized by comprising bacillus subtilis (Bacillus subtilis) Y1 and bacillus belicus (Bacillus velezensis) Y3, wherein the bacillus subtilis Y1 is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022, 2 and 28 days and a preservation number of CGMCC NO.24449; the bacillus bailii Y3 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2022, 2 and 28 days and a preservation number of CGMCC NO.24450.
6. The kitchen waste decomposing and degrading bacterial agent according to claim 5, wherein the ratio of bacillus subtilis Y1 to bacillus bailii Y3 is 2:1.
7. The preparation method of the kitchen waste decomposing and degrading microbial inoculum of claim 5, which is characterized in that bacillus subtilis Y1 and bacillus beijerinckii Y3 are inoculated in a ratio of Y1 to Y3 of 2:1, the inoculation amount is 10-20%, and the culture is carried out under the conditions that the rotation speed is 120-160 r/min, the pH is 7-8 and the fermentation temperature is 30 ℃ to obtain the kitchen waste decomposing and degrading microbial inoculum.
8. The preparation method of the kitchen waste decomposing degradation microbial inoculum of claim 7, wherein the inoculation amount is 13.5%, the rotating speed is 132r/min, and the pH is 7.6.
9. A kitchen waste decomposition degradation method, which is characterized in that the kitchen waste decomposition degradation microbial inoculum of claim 5 is mixed with kitchen waste and subjected to aerobic fermentation.
10. The kitchen waste decomposing and degrading method as claimed in claim 9, wherein the bacterial load is 200g/t and the rotation speed is 12r/min; the initial temperature is 60 ℃; the fermentation time was 16h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310846392.2A CN116875501A (en) | 2022-03-22 | 2022-03-22 | Kitchen waste decomposing degradation microbial inoculum and preparation method and application thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210282942.8A CN114806926B (en) | 2022-03-22 | 2022-03-22 | Kitchen waste decomposing degradation microbial inoculum and application thereof |
| CN202310846392.2A CN116875501A (en) | 2022-03-22 | 2022-03-22 | Kitchen waste decomposing degradation microbial inoculum and preparation method and application thereof |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210282942.8A Division CN114806926B (en) | 2022-03-22 | 2022-03-22 | Kitchen waste decomposing degradation microbial inoculum and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116875501A true CN116875501A (en) | 2023-10-13 |
Family
ID=82530739
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310846392.2A Pending CN116875501A (en) | 2022-03-22 | 2022-03-22 | Kitchen waste decomposing degradation microbial inoculum and preparation method and application thereof |
| CN202210282942.8A Active CN114806926B (en) | 2022-03-22 | 2022-03-22 | Kitchen waste decomposing degradation microbial inoculum and application thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210282942.8A Active CN114806926B (en) | 2022-03-22 | 2022-03-22 | Kitchen waste decomposing degradation microbial inoculum and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN116875501A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117844691A (en) * | 2023-12-29 | 2024-04-09 | 北京舞鹤环境工程设计有限公司 | Strain for pretreatment of kitchen waste with high water content and high fiber content and application thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103589671B (en) * | 2013-11-12 | 2015-08-19 | 无锡亚克生物科技有限公司 | A kind of subtilis LNXM37 and application thereof |
| CN110331109B (en) * | 2019-07-24 | 2020-11-03 | 山东省农业科学院农业资源与环境研究所 | Bacillus subtilis and culture method and application thereof |
| CN112481176A (en) * | 2021-01-11 | 2021-03-12 | 山西省农业科学院农业环境与资源研究所 | Decomposing inoculant for branch composting and preparation method thereof |
| CN113151082B (en) * | 2021-04-21 | 2022-05-17 | 中国科学院东北地理与农业生态研究所 | Multifunctional soil bacterium and application thereof |
| CN113444657A (en) * | 2021-05-20 | 2021-09-28 | 华东师范大学 | Solid-state microbial composite microbial agent for promoting aerobic composting of kitchen waste and preparation and application thereof |
-
2022
- 2022-03-22 CN CN202310846392.2A patent/CN116875501A/en active Pending
- 2022-03-22 CN CN202210282942.8A patent/CN114806926B/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117844691A (en) * | 2023-12-29 | 2024-04-09 | 北京舞鹤环境工程设计有限公司 | Strain for pretreatment of kitchen waste with high water content and high fiber content and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114806926A (en) | 2022-07-29 |
| CN114806926B (en) | 2023-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111893056B (en) | Bacillus belgii KY01 and application thereof in degrading kitchen garbage | |
| JP7223808B2 (en) | Microbial inoculant for high-temperature biodegradation of garbage and method of decomposition of garbage | |
| CN110643544B (en) | Lactobacillus paracasei761, application thereof, silage additive and silage | |
| CN110591987B (en) | Lactobacillus salivarius358 and application thereof, silage additive and silage | |
| CN108949739A (en) | A kind of complex micro organism fungicide and preparation method thereof for advanced treating high concentration livestock breeding wastewater | |
| CN103602616A (en) | Bacillus amyloliquefaciens Y10 and application thereof | |
| CN105524858A (en) | High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum | |
| CN109402013B (en) | High-temperature-resistant bacillus subtilis for producing lipase and application of bacillus subtilis in composting | |
| CN114480205B (en) | Bacillus amyloliquefaciens and application thereof in brewing of solid-state fermentation vinegar | |
| CN114806926B (en) | Kitchen waste decomposing degradation microbial inoculum and application thereof | |
| CN108865927B (en) | Bacterial strain for low-temperature glycolysis of corn straw and fermentation culture method and application thereof | |
| CN111893065B (en) | Low-temperature cellulose degradation bacterium | |
| CN116463270B (en) | Lignocellulose degrading microbial agent and preparation method and application thereof | |
| CN111154661B (en) | Complex microbial inoculant and application thereof | |
| CN112625948A (en) | Bacillus tequilensis S1 with nitrogen fixation function and application thereof in compost | |
| CN108841743B (en) | Cold region straw rotten bacterial strain and preparation method and application thereof | |
| CN114480201B (en) | Vibrio natriurus capable of strongly degrading enteromorpha protein and application of vibrio natriurus in preparation of organic fertilizer | |
| CN114717125B (en) | Thermophilic bacillus licheniformis AMCC101380 and application thereof in high-temperature composting of tail vegetables | |
| CN112280708B (en) | Cellulose degradation composite microbial inoculum and application thereof | |
| CN109609412A (en) | A kind of Thermophilic Bacteria Bacillus smithii Ths1 and its application | |
| CN108913629A (en) | A kind of bacterium of cellulase-producing and the preparation method and application thereof | |
| CN114437976A (en) | Compound microbial agent and application thereof in biological reduction of kitchen waste | |
| CN111172062B (en) | Sphingobacterium multivorum and application thereof | |
| CN114921356A (en) | Household kitchen waste aerobic composting composite microbial agent and preparation method thereof | |
| CN112662577A (en) | Flora for degrading corn straw to produce polysaccharide and microorganism proportion thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |