CN116769595A - Low-pressure low-oxygen cell simulator - Google Patents
Low-pressure low-oxygen cell simulator Download PDFInfo
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- CN116769595A CN116769595A CN202310723443.2A CN202310723443A CN116769595A CN 116769595 A CN116769595 A CN 116769595A CN 202310723443 A CN202310723443 A CN 202310723443A CN 116769595 A CN116769595 A CN 116769595A
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- 239000001301 oxygen Substances 0.000 title abstract description 21
- 229910052760 oxygen Inorganic materials 0.000 title abstract description 21
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 52
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 45
- 210000000170 cell membrane Anatomy 0.000 claims abstract description 27
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 26
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 26
- 238000004088 simulation Methods 0.000 claims abstract description 22
- 210000004027 cell Anatomy 0.000 claims abstract description 21
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 19
- 238000004378 air conditioning Methods 0.000 claims description 8
- 229910001873 dinitrogen Inorganic materials 0.000 claims description 7
- 239000007789 gas Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 6
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 206010021143 Hypoxia Diseases 0.000 claims description 4
- 230000001146 hypoxic effect Effects 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 abstract description 35
- 238000002474 experimental method Methods 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 8
- 230000001575 pathological effect Effects 0.000 abstract description 5
- 230000001105 regulatory effect Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000005259 measurement Methods 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000009530 blood pressure measurement Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- 238000010171 animal model Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 230000001364 causal effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000009134 cell regulation Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000036647 reaction Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The application belongs to the technical field of pathological experiment simulation devices, and particularly relates to a low-pressure low-oxygen cell simulation device which comprises a base, a computer and a temperature raising device; the top of the base is provided with a cabinet body, and the front surface of the cabinet body is provided with a cabinet door; the computer is arranged on one side of the cabinet body, a printer is further arranged on one side of the cabinet body close to the computer, the printer is connected with the computer, a temperature and humidity measuring and controlling device, a pressure measuring and controlling device, a nitrogen concentration measuring and controlling device and a carbon dioxide concentration measuring and controlling device are respectively arranged at the top of the cabinet body, and the temperature and humidity measuring and controlling device, the pressure measuring and controlling device, the nitrogen concentration measuring and controlling device and the carbon dioxide concentration measuring and controlling device are all connected with the computer; the cell membrane plate rack has the advantages of reasonable structure, convenience in accurately regulating and controlling the experimental environment in the use process, convenience in regulating the distance between the cell membrane plate racks according to the differences of experimental objects, high flexibility, wide practical range and strong practicability, and is beneficial to improving the efficiency and accuracy of experiments.
Description
Technical Field
The application relates to the technical field of pathological experiment simulation devices, in particular to a low-pressure low-oxygen cell simulation device.
Background
Pathological studies under low pressure and low oxygen conditions animals have been used in the past to perform the required simulation. The required device and materials occupy larger space, the required device is complicated and expensive, and the control precision is difficult to meet the requirement. The low-pressure hypoxia simulation experiment performed by adopting cells to replace animals along with the scientific development is an emerging experimental method in pathological research under the low-pressure hypoxia condition in the current medical science; the development of a low-pressure low-oxygen environment simulation experiment device for the research is an urgent need.
Among the prior art, the application number is CN201120401522.4, and the application name is cell low pressure low oxygen environment simulation experiment device, among the described technical scheme, including having the sealed cabinet body wholly by thermal-insulated insulating properties material preparation, the sealed cabinet body and the inside surface material of sealing door are by corrosion-resistant and be convenient for clean, are provided with movable hinge connection between the sealed cabinet body and the sealing door, install joint strip all around, guarantee sealed good. The incubator is arranged at the bottom of the cabinet, a plurality of layers of cell membrane plates are arranged at the upper part of the incubator, the cell membrane plates are made of metal materials with heat conduction performance, the controlled cell growth environment is ensured to be at 37 ℃, the lower part of each layer of cell membrane plate is provided with a nutrient solution supply device and is connected through a nutrient solution supply pipeline, the cell membrane plates are directly placed on the cell membrane plate, and the cell membranes of the cell membrane plates are directly immersed in the nutrient solution supply device; the top in the sealed cabinet body is provided with ozone sterilizer and ultraviolet sterilizing lamp, and cabinet body upper end is provided with temperature measurement and control ware, humidity measurement and control ware, pressure measurement and control ware, nitrogen concentration measurement and control ware and carbon dioxide concentration measurement and control ware all are connected to the computer, and external connection takes the nitrogen gas source of nitrogen flow control valve and takes carbon dioxide gas source and the air conditioner circulating pump and the evacuation pump of carbon dioxide flow control valve also to be connected with the computer. The low-pressure low-oxygen environment simulation experiment using the cells is used for replacing the low-pressure low-oxygen environment simulation experiment using animals, and because the equipment used for the experiment using the cells is smaller than the experimental equipment used for the animal experiment, the experimental precision can be better controlled, various interference factors can be better shielded, the causal relationship of the cells, such as damage and treatment methods caused by the low-pressure low-oxygen can be more accurately inspected, and the consumed raw materials and time are greatly saved; the method has the advantages that the effect of directly expressing the cell reaction is scientific and strict, experimental animals are not used, the experimental cost and time are saved, and the experimental efficiency is greatly improved; provides new automatic equipment for the new experimental method in pathological research under the condition of low pressure and low oxygen in the current medical science, and can be widely applied to the related medical science research.
However, in the practical use process, some defects still exist, such as in the practical process, the distance between cell membrane grillages is inconvenient to adjust according to the differences of experimental objects, the flexibility is low, the application range is small, the practicability is poor, the fixing of the plate culture dish is inconvenient, the stability is poor, the safety is low, and the normal development of experiments is affected.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.
The present application has been made in view of the problems occurring in the prior art.
Therefore, the application aims to provide the low-pressure low-oxygen cell simulation device, which is convenient for accurately regulating and controlling the experimental environment in the use process, and simultaneously is convenient for regulating the distance between cell membrane grillage according to different experimental objects, has high flexibility, wide practical range and strong practicability, and is beneficial to improving the efficiency and accuracy of experiments.
In order to solve the technical problems, according to one aspect of the present application, the following technical solutions are provided:
a low-pressure low-oxygen cell simulation device comprises a base, a computer and a temperature incubator;
wherein,,
the top of the base is provided with a cabinet body, and the front surface of the cabinet body is provided with a cabinet door;
the computer is arranged on one side of the cabinet body, a printer is further arranged on one side of the cabinet body close to the computer, the printer is connected with the computer, a temperature and humidity measuring and controlling device, a pressure measuring and controlling device, a nitrogen concentration measuring and controlling device and a carbon dioxide concentration measuring and controlling device are respectively arranged at the top of the cabinet body, the temperature and humidity measuring and controlling device, the pressure measuring and controlling device, the nitrogen concentration measuring and controlling device and the carbon dioxide concentration measuring and controlling device are all connected with the computer, an air-conditioning circulating pump, a vacuum pump, a carbon dioxide air source and a nitrogen air source are respectively arranged on two sides of the top of the base, and the air-conditioning circulating pump, the vacuum pump, the carbon dioxide air source and the nitrogen air source are all connected with the computer;
the incubator is arranged at the bottom of an inner cavity of the cabinet body, fixing frames are arranged at four corners of the top of the incubator, sliding sleeves are arranged on the surfaces of the fixing frames, locking knobs are arranged on one sides of the sliding sleeves, cell membrane grillage frames are arranged between the sliding sleeves, fixing sleeves are arranged at the tops of the cell membrane grillage frames, elastic pieces are arranged on the side walls of the inner cavity of the fixing sleeves, and locking clamping blocks are arranged at one ends of the elastic pieces.
As a preferred embodiment of the low-pressure low-oxygen cell simulation apparatus according to the present application, wherein: the bottom four corners of base are provided with the support foot rest, and the bottom of support foot rest is provided with anti-skidding gasket.
As a preferred embodiment of the low-pressure low-oxygen cell simulation apparatus according to the present application, wherein: the front surface of cabinet door is provided with the observation window, and is provided with the door handle on the cabinet door.
As a preferred embodiment of the low-pressure low-oxygen cell simulation apparatus according to the present application, wherein: and flow control valves are arranged on the carbon dioxide gas source and the nitrogen gas source.
As a preferred embodiment of the low-pressure low-oxygen cell simulation apparatus according to the present application, wherein: an ozone sterilizer and an ultraviolet sterilizing lamp are arranged at the top of the inner cavity of the cabinet body.
As a preferred embodiment of the low-pressure low-oxygen cell simulation apparatus according to the present application, wherein: one side of the fixing frame is provided with a locking screw hole matched with the locking knob.
As a preferred embodiment of the low-pressure low-oxygen cell simulation apparatus according to the present application, wherein: the elastic piece is a reset spring, the locking clamping blocks are obliquely arranged, and rubber gaskets are arranged on the surfaces of the locking clamping blocks.
As a preferred embodiment of the low-pressure low-oxygen cell simulation apparatus according to the present application, wherein: the top of the cell membrane grillage is also provided with a nutrient solution supply component.
Compared with the prior art, the application has the beneficial effects that: through being provided with the sliding sleeve on the mount, be provided with locking knob on the sliding sleeve, be provided with matched with locking screw simultaneously on the mount, adjust the position of sliding sleeve on the mount through locking knob and locking screw, can realize the interval regulation of cell membrane grillage, the convenience is according to the interval of the different adjustment cell membrane grillages of experimental object, and the flexibility is high, and the practical range is wide, and the practicality is strong, helps improving the efficiency and the accuracy of experiment.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following detailed description of the embodiments of the present application will be given with reference to the accompanying drawings, which are to be understood as merely some embodiments of the present application, and from which other drawings can be obtained by those skilled in the art without inventive faculty. Wherein:
FIG. 1 is a schematic diagram of the structure of the present application;
FIG. 2 is a schematic diagram of the internal structure of the present application;
FIG. 3 is a schematic top view of the cell membrane panel rack of the present application;
fig. 4 is a schematic structural view of the fixing sleeve of the present application.
In the figure; 100 bases, 110 supporting foot frames, 120 cabinet bodies, 130 cabinet doors, 200 computers, 210 printers, 220 temperature and humidity measuring and controlling devices, 230 pressure measuring and controlling devices, 240 nitrogen concentration measuring and controlling devices, 250 carbon dioxide concentration measuring and controlling devices, 260 air conditioning circulating pumps, 270 vacuum pumps, 180 carbon dioxide gas sources, 290 nitrogen gas sources, 300 temperature raising devices, 310 fixing frames, 320 sliding sleeves, 330 cell membrane plate frames, 340 locking knobs, 350 fixing sleeves, 360 elastic pieces and 370 locking clamping blocks.
Detailed Description
In order that the above objects, features and advantages of the application will be readily understood, a more particular description of the application will be rendered by reference to the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, but the present application may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present application is not limited to the specific embodiments disclosed below.
Next, the present application will be described in detail with reference to the drawings, wherein the sectional view of the device structure is not partially enlarged to general scale for the convenience of description, and the drawings are only examples, which should not limit the scope of the present application. In addition, the three-dimensional dimensions of length, width and depth should be included in actual fabrication.
For the purpose of making the objects, technical solutions and advantages of the present application more apparent, embodiments of the present application will be described in further detail below with reference to the accompanying drawings.
The application provides the following technical scheme: the low-pressure low-oxygen cell simulation device is convenient for accurately regulating and controlling the experimental environment in the use process, and simultaneously, the distance between cell membrane grillages is convenient to adjust according to different experimental objects, so that the device is high in flexibility, wide in practical range and strong in practicability, and is beneficial to improving the efficiency and accuracy of experiments;
FIGS. 1 to 4 are schematic views showing the structure of an embodiment of a low pressure hypoxic cell simulation apparatus according to the application, the main body part of which comprises a base 100, a computer 200 and a incubator 300;
please refer again to fig. 1-4: the top of the base 100 is welded with a cabinet body 120, the front surface of the cabinet body 120 is in threaded connection with a cabinet door 130, four corners of the bottom of the base 100 are welded with support foot frames 110, and the bottoms of the support foot frames 110 are adhered with anti-slip gaskets; an observation window is formed in the front surface of the cabinet door 130, a door handle is screwed on the cabinet door 130, an ozone sterilizer and an ultraviolet sterilizing lamp are screwed on the top of an inner cavity of the cabinet body 120, the ozone sterilizer and the ultraviolet sterilizing lamp are used for realizing sterilization, the cabinet body 120 is supported by the base 100 and the supporting foot stand 110, the cabinet body 120 is used for providing a culture environment, and the cabinet door 130 is used for sealing the cabinet body;
please refer again to fig. 1-4: the computer 200 is screwed on one side of the cabinet body 120, the printer 210 is also screwed on one side of the cabinet body 120 close to the computer 200, the printer 210 is connected with the computer 200, the top of the cabinet body 120 is respectively screwed with the temperature and humidity measuring and controlling device 220, the pressure measuring and controlling device 230, the nitrogen concentration measuring and controlling device 240 and the carbon dioxide concentration measuring and controlling device 250, the temperature and humidity measuring and controlling device 220, the pressure measuring and controlling device 230, the nitrogen concentration measuring and controlling device 240 and the carbon dioxide concentration measuring and controlling device 250 are all connected with the computer 200, the air conditioning circulating pump 260, the vacuum pump 270, the carbon dioxide air source 280 and the nitrogen air source 290 are respectively screwed on two sides of the top of the base 100, the air conditioning circulating pump 260, the vacuum pump 270, the carbon dioxide gas source 280 and the nitrogen gas source 290 are all connected with the computer 200, the carbon dioxide gas source 280 and the nitrogen gas source 290 are both connected with flow control valves in a screwed mode, the computer 200 is used for circuit control, the printer 210 is used for data printing, the temperature and humidity measuring and controlling device 220 is used for temperature and humidity measurement and control, the pressure measuring and controlling device 230 is used for pressure measurement and control, the nitrogen concentration measuring and controlling device 240 is used for nitrogen concentration measurement and control, the carbon dioxide concentration measuring and controlling device 250 is used for carbon dioxide concentration measurement and control, the air conditioning circulating pump 260 is used for adjusting temperature and humidity, the vacuum pump 270 is used for adjusting pressure, the carbon dioxide gas source 280 is used for adjusting carbon dioxide concentration, and the nitrogen gas source 290 is used for adjusting nitrogen concentration;
please refer again to fig. 1-4: the temperature raising device 300 is in threaded connection with the bottom of the inner cavity of the cabinet body 120, a fixing frame 310 is welded at four corners of the top of the temperature raising device 300, a sliding sleeve 320 is sleeved on the surface of the fixing frame 310, a locking knob 340 is in threaded connection with one side of the sliding sleeve 320, a locking screw hole matched with the locking knob 340 is formed in one side of the fixing frame 310, a cell membrane plate frame 330 is welded between the sliding sleeves 320, a fixing sleeve 350 is welded at the top of the cell membrane plate frame 330, an elastic piece 360 is in threaded connection with the inner cavity side wall of the fixing sleeve 350, one end of the elastic piece 360 is in threaded connection with a locking clamp block 370, the elastic piece 360 is a reset spring, the locking clamp block 370 is in inclined arrangement, a rubber gasket is adhered to the surface of the locking clamp block 370, the temperature raising device 300 is used for temperature regulation, the fixing frame 310 is used for installing the sliding sleeve 320, the sliding sleeve 320 is used for sliding up and down and bearing the cell membrane plate frame 330, the locking knob 340 is used for fixing the position of the sliding sleeve 320, and the fixing sleeve 350, the elastic piece 360 and the locking clamp block 370 are used for fixing a test tube, and stability is good.
Working principle: in the use process of the application, the sliding sleeve 320 is arranged on the fixed frame 310, the locking knob 340 is arranged on the sliding sleeve 320, meanwhile, the matched locking screw hole is arranged on the fixed frame 310, the position of the sliding sleeve 320 on the fixed frame 310 is adjusted through the locking knob 340 and the locking screw hole, the spacing adjustment of the cell membrane grillage 330 can be realized, the spacing adjustment of the cell membrane grillage 330 is convenient to adjust according to different experimental objects, the flexibility is high, the practical range is wide, the practicability is strong, and the efficiency and the accuracy of experiments are improved.
Although the application has been described hereinabove with reference to embodiments, various modifications thereof may be made and equivalents may be substituted for elements thereof without departing from the scope of the application. In particular, the features of the disclosed embodiments may be combined with each other in any manner as long as there is no structural conflict, and the exhaustive description of these combinations is not given in this specification merely for the sake of omitting the descriptions and saving resources. Therefore, it is intended that the application not be limited to the particular embodiment disclosed, but that the application will include all embodiments falling within the scope of the appended claims.
Claims (8)
1. A low pressure hypoxic cell simulation apparatus, characterized in that: comprises a base (100), a computer (200) and a thermostat (300);
wherein,,
the top of the base (100) is provided with a cabinet body (120), and the front surface of the cabinet body (120) is provided with a cabinet door (130);
the computer (200) is arranged on one side of the cabinet body (120), a printer (210) is further arranged on one side, close to the computer (200), of the cabinet body (120), the printer (210) is connected with the computer (200), a temperature and humidity measuring and controlling device (220), a pressure measuring and controlling device (230), a nitrogen concentration measuring and controlling device (240) and a carbon dioxide concentration measuring and controlling device (250) are respectively arranged at the top of the cabinet body (120), the temperature and humidity measuring and controlling device (220), the pressure measuring and controlling device (230), the nitrogen concentration measuring and controlling device (240) and the carbon dioxide concentration measuring and controlling device (250) are all connected with the computer (200), an air conditioning circulating pump (260), a vacuum pump (270), a carbon dioxide air source (280) and a nitrogen air source (290) are respectively arranged on two sides of the top of the base (100), and the air conditioning circulating pump (260), the vacuum pump (270), the carbon dioxide air source (280) and the nitrogen air source (290) are all connected with the computer (200).
The temperature raising device is characterized in that the temperature raising device (300) is arranged at the bottom of an inner cavity of the cabinet body (120), fixing frames (310) are arranged at four corners of the top of the temperature raising device (300), sliding sleeves (320) are arranged on the surfaces of the fixing frames (310), locking knobs (340) are arranged on one sides of the sliding sleeves (320), cell membrane grillage (330) are arranged between the sliding sleeves (320), fixing sleeves (350) are arranged at the top of the cell membrane grillage (330), elastic pieces (360) are arranged on the side walls of the inner cavities of the fixing sleeves (350), and locking clamping blocks (370) are arranged at one ends of the elastic pieces (360).
2. The device according to claim 1, wherein: the four corners of the bottom of the base (100) are provided with supporting foot frames (110), and the bottoms of the supporting foot frames (110) are provided with anti-slip gaskets.
3. The device according to claim 1, wherein: the front surface of cabinet door (130) is provided with the observation window, and is provided with the door handle on cabinet door (130).
4. The device according to claim 1, wherein: flow control valves are arranged on the carbon dioxide gas source (280) and the nitrogen gas source (290).
5. The device according to claim 1, wherein: an ozone sterilizer and an ultraviolet sterilizing lamp are arranged at the top of the inner cavity of the cabinet body (120).
6. The device according to claim 1, wherein: one side of the fixing frame (310) is provided with a locking screw hole matched with the locking knob (340).
7. The device according to claim 1, wherein: the elastic piece (360) is a return spring, the locking clamping block (370) is obliquely arranged, and a rubber gasket is arranged on the surface of the locking clamping block (370).
8. The device according to claim 1, wherein: the top of the cell membrane plate rack (330) is also provided with a nutrient solution supply assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310723443.2A CN116769595A (en) | 2023-06-19 | 2023-06-19 | Low-pressure low-oxygen cell simulator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310723443.2A CN116769595A (en) | 2023-06-19 | 2023-06-19 | Low-pressure low-oxygen cell simulator |
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| CN116769595A true CN116769595A (en) | 2023-09-19 |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202279825U (en) * | 2011-10-20 | 2012-06-20 | 天津开发区合普工贸有限公司 | Simulated experiment device for cell low-pressure hypoxia condition |
| KR20130139051A (en) * | 2012-06-12 | 2013-12-20 | 고광진 | Multifunctional incubator |
| CN208131087U (en) * | 2018-01-18 | 2018-11-23 | 唐琼娥 | A kind of height-adjustable breakage-proof rack for test tube of rotary medical test |
| CN110903975A (en) * | 2019-12-16 | 2020-03-24 | 陈志勇 | Adjustable cell culture tray placing frame and adjusting method |
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| CN112725178A (en) * | 2021-02-10 | 2021-04-30 | 上海塔望智能科技有限公司 | Cell hypoxia high-pressure culture device |
| CN214234136U (en) * | 2020-12-02 | 2021-09-21 | 江苏曼格尼特磁电有限公司 | Processing apparatus of magnetic material research for environmental pollution detects |
| CN214229613U (en) * | 2020-10-26 | 2021-09-21 | 武汉目光生物科技有限公司 | Stem cell test tube storage device is used in laboratory |
| CN216906315U (en) * | 2022-04-11 | 2022-07-08 | 河南科技学院 | Full artifical light plant factory floor height adjustable assembled fog cultivation plants layer frame |
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2023
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| CN202279825U (en) * | 2011-10-20 | 2012-06-20 | 天津开发区合普工贸有限公司 | Simulated experiment device for cell low-pressure hypoxia condition |
| KR20130139051A (en) * | 2012-06-12 | 2013-12-20 | 고광진 | Multifunctional incubator |
| CN208131087U (en) * | 2018-01-18 | 2018-11-23 | 唐琼娥 | A kind of height-adjustable breakage-proof rack for test tube of rotary medical test |
| CN210765329U (en) * | 2019-07-29 | 2020-06-16 | 西安医学院 | Low-pressure and low-oxygen cell experimental device |
| CN110903975A (en) * | 2019-12-16 | 2020-03-24 | 陈志勇 | Adjustable cell culture tray placing frame and adjusting method |
| CN214229613U (en) * | 2020-10-26 | 2021-09-21 | 武汉目光生物科技有限公司 | Stem cell test tube storage device is used in laboratory |
| CN214234136U (en) * | 2020-12-02 | 2021-09-21 | 江苏曼格尼特磁电有限公司 | Processing apparatus of magnetic material research for environmental pollution detects |
| CN112725178A (en) * | 2021-02-10 | 2021-04-30 | 上海塔望智能科技有限公司 | Cell hypoxia high-pressure culture device |
| CN216906315U (en) * | 2022-04-11 | 2022-07-08 | 河南科技学院 | Full artifical light plant factory floor height adjustable assembled fog cultivation plants layer frame |
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