CN116694465A - Cell culture device capable of monitoring cell culture liquid environment - Google Patents

Cell culture device capable of monitoring cell culture liquid environment Download PDF

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Publication number
CN116694465A
CN116694465A CN202310610703.5A CN202310610703A CN116694465A CN 116694465 A CN116694465 A CN 116694465A CN 202310610703 A CN202310610703 A CN 202310610703A CN 116694465 A CN116694465 A CN 116694465A
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cell culture
environment
culture
liquid
monitoring
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李战战
刘爱斌
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Xiangya Hospital of Central South University
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Xiangya Hospital of Central South University
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Priority to CN202310610703.5A priority Critical patent/CN116694465A/en
Publication of CN116694465A publication Critical patent/CN116694465A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

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Abstract

The utility model provides a cell culture device capable of monitoring cell culture liquid environment, which relates to the technical field of cell culture and comprises the following components: cultivate the mechanism, sealing mechanism activity is inlayed on cultivateing the mechanism, and monitoring mechanism is fixed to be set up in cultivateing the inside of mechanism and sealing mechanism, through cultivateing the mutual installation of mechanism and sealing mechanism for monitoring mechanism switches on, and then better carries out real-time supervision to cultivateing the liquid environment in the mechanism, adds liquid mechanism intercommunication and sets up on sealing mechanism, adds liquid mechanism and adds the culture solution in the mechanism to cultivateing that liquid mechanism can be better, does not need to open culture apparatus. The utility model solves the problems that the culture solution is required to be extracted and the culture device is required to be opened when the culture solution is added during the environment monitoring of the culture solution, and the culture solution added in the culture mechanism can be better detected through the monitoring mechanism, so that the change of the environment of the culture solution during the cell culture can be better reflected.

Description

Cell culture device capable of monitoring cell culture liquid environment
Technical Field
The utility model relates to the technical field of cell culture, in particular to a cell culture device capable of monitoring the liquid environment of cell culture.
Background
The cell in vitro culture technology is to inoculate mammalian cells into a liquid culture medium in a cell culture container, wherein the liquid culture medium contains various nutrient components necessary for the growth and propagation of the cells, so that the cells grow and proliferate. In scientific research, it is often necessary to study the effect of a specific substance in a cell culture solution on the processes of cell growth, division, proliferation and the like at a specific concentration.
The current Chinese patent of the utility model with the publication number of CN216404420U provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises a culture dish, wherein a threaded ring is fixed on the upper side of the culture dish, a threaded rod is rotatably arranged in the threaded ring, a sampling tube is arranged on the lower side of the threaded rod, a sampling tube matched with the culture dish in position is fixed on the outer end face of the sampling tube, an air pump is fixed on the upper end of the sampling tube, a conveying pipe is fixed on the lower end of the culture dish, and an observation plate is connected on the outer end of the conveying pipe.
By adopting the technical scheme, the culture solution in the culture dish can be sampled, the cells are prevented from being picked up, normal growth of the cells is guaranteed, the conventional cell culture device can be seen by the technical scheme to be pulled out of the culture solution when detecting the environment of the culture solution, the growth of the cells is not facilitated due to the reduction of the culture solution in the pulling out process, the cultured cells are easily carried out in the extracting process of the culture solution, the loss is caused by the reduction of the quantity of the cultured cells, the culture device is often opened when the culture solution is added, and the cultured cells are easily polluted.
Disclosure of Invention
The utility model aims to provide a cell culture device capable of monitoring the environment of cell culture liquid, which solves the problems that the culture device needs to be opened when the culture liquid is extracted and added during the monitoring of the environment of the culture liquid.
The technical aim of the utility model is realized by the following technical scheme:
a cell culture apparatus for monitoring the environment of a cell culture fluid, comprising:
a culturing mechanism;
the sealing mechanism is movably embedded on the culture mechanism;
the monitoring mechanism is fixedly arranged in the culture mechanism and the sealing mechanism, and is connected through the mutual installation of the culture mechanism and the sealing mechanism, so that the environment of the culture solution in the culture mechanism is better monitored in real time;
and the liquid adding mechanism is communicated with the sealing mechanism, and can better add culture liquid into the culture mechanism without opening the culture device.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the monitoring mechanism comprises a PH sensor and an osmotic pressure detection component, wherein the PH sensor is fixedly arranged at the bottom end of the culture mechanism, and the osmotic pressure detection component is fixedly arranged at the outer side of the culture mechanism.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the utility model discloses a PH sensor, including PH sensor, osmotic pressure detection component, the intercommunication mouth has been seted up at the adjacent culture mechanism top of PH sensor and osmotic pressure detection component, the inside bottom mounting of intercommunication mouth sets up the joint electric tank, the joint electric tank carries out electric connection with PH sensor and osmotic pressure detection component.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the bottom of sealing mechanism corresponds the fixed joint electric plate that is provided with of intercommunication mouth, joint electric plate and joint electric tank carry out the electric connection when sealing mechanism and cultivation mechanism assemble.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the utility model discloses a sealing mechanism, including sealing mechanism, controller, singlechip, joint electric sheet, sealing mechanism's top surface is fixed to be provided with the display screen, one side of display screen is provided with the observation window, sealing mechanism's inside is fixed to be provided with singlechip and controller, the singlechip carries out electric connection with the display screen, the controller electric setting is in the both sides of singlechip, two electric connection is carried out with the joint electric sheet to the controller.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the liquid adding mechanism comprises a liquid adding port and a blanking ring, the liquid adding port is formed in the top of the sealing mechanism, and the blanking ring is fixedly arranged at the bottom of the sealing mechanism.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the inside top of adding the liquid mouth is fixed and is provided with sealed lamella, the stock solution chamber has been seted up to the inside of unloading ring, the below in stock solution chamber runs through unloading ring bottom and is provided with the liquid outlet, it communicates with the stock solution chamber to add the material mouth.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the osmotic pressure detection component comprises a laser ranging sensor and a reflecting sheet, a detection cavity is formed in the osmotic pressure detection component, the laser ranging sensor is fixedly arranged at the top of the detection cavity, and the reflecting sheet is arranged below the detection cavity corresponding to the laser ranging sensor.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the inner wall of the detection cavity is provided with a sliding groove, and the reflecting sheet is arranged in the sliding groove in a sliding way.
The utility model provides a cell culture device capable of monitoring the cell culture liquid environment, which comprises: the osmotic pressure detection component below communicates with the inside of the culture mechanism, and a semipermeable membrane is fixedly arranged in the communicated inside.
In summary, the beneficial technical effects of the utility model are as follows:
(1) Utilize detection mechanism, detection mechanism includes PH sensor and osmotic pressure detection component, through setting up PH sensor and osmotic pressure detection component in culture mechanism's inside bottom and outside respectively, carry out electric connection with PH sensor and osmotic pressure detection component and joint electric tank simultaneously, secondly set up the joint electric plate in sealing mechanism bottom, set up singlechip and controller simultaneously in sealing mechanism, carry out electric connection with joint electric plate and controller, and then assemble sealing mechanism and culture mechanism back, make the joint electric tank be connected with the joint electric plate, and then put through PH sensor and osmotic pressure detection component and controller, thereby better convenience is through singlechip and controller, can show the environmental change of culture apparatus inside culture solution on the display screen in real time, and then make the monitoring more convenient, can not cause the loss of cultured cell.
(2) Utilize to add liquid mechanism, add liquid mechanism and include to add liquid mouth and unloading ring, through seting up the liquid mouth of adding in sealing mechanism's top, set up the unloading ring in sealing mechanism's below, and then make when adding the culture solution, need not open culture apparatus, directly add the culture solution through adding the liquid mouth to reduced the contact of cell and the outside air of culture, and then better protection cell is not polluted, makes simultaneously more convenient when adding the culture solution.
Additional aspects and advantages of the utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model.
Drawings
In order to more clearly illustrate the utility model or the technical solutions of the prior art, the following description will briefly explain the embodiments or the drawings needed in the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the utility model and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic overall structure of an embodiment of the present utility model;
FIG. 2 is a schematic structural development of an embodiment of the present utility model;
FIG. 3 is a schematic view of the bottom structure of an embodiment of the present utility model;
FIG. 4 is a top structural plan view of an embodiment of the present utility model;
FIG. 5 is a side structural plan view of an embodiment of the present utility model;
FIG. 6 is a cross-sectional side view of an embodiment of the present utility model;
FIG. 7 is an enlarged view of FIG. 6 at A;
fig. 8 is a schematic diagram of the working principle of an embodiment of the present utility model.
Reference numerals:
1. a culturing mechanism; 10. a communication port; 101. a clamping electric groove;
2. a sealing mechanism; 201. clamping the electric sheet; 202. a controller; 203. a single chip microcomputer; 204. a display screen;
4. an observation window;
5. an osmotic pressure detecting member; 501. a detection chamber; 502. a sliding groove; 503. a reflection sheet; 504. a laser ranging sensor; 505. a semipermeable membrane;
6. a liquid adding mechanism; 601. a liquid adding port; 602. sealing petals; 603. a blanking ring; 604. a liquid storage cavity; 605. a liquid outlet;
7. PH sensor.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the embodiments of the present utility model, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely for convenience in describing the embodiments of the present utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the embodiments of the present utility model. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In describing embodiments of the present utility model, it should be noted that, unless explicitly stated and limited otherwise, the terms "coupled," "coupled," and "connected" should be construed broadly, and may be either a fixed connection, a removable connection, or an integral connection, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in embodiments of the present utility model will be understood in detail by those of ordinary skill in the art.
In embodiments of the utility model, unless expressly specified and limited otherwise, a first feature "up" or "down" on a second feature may be that the first and second features are in direct contact, or that the first and second features are in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the embodiments of the present utility model. In this specification, schematic representations of the above terms are not directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
The technical solution of the present utility model is described below with reference to the embodiments shown in fig. 1 to 8:
a cell culture apparatus for monitoring the environment of a cell culture fluid, comprising: cultivate mechanism 1, sealing mechanism 2 activity is inlayed on cultivateing mechanism 1, and monitoring mechanism is fixed to be set up in cultivateing mechanism 1 and sealing mechanism 2's inside, through cultivateing mechanism 1 and sealing mechanism 2's mutual installation for monitoring mechanism switches on, and then better carries out real-time supervision to cultivateing the liquid environment in cultivateing mechanism 1, adds liquid mechanism 6 intercommunication setting on sealing mechanism 2, adds liquid mechanism 6 and adds the culture solution in the cultivation mechanism 1 that can be better, does not need to open culture apparatus.
It will be appreciated that the detection mechanism includes a PH sensor 7 and an osmotic pressure detection member 5, and that the liquid adding mechanism 6 includes a liquid adding port 601 and a blanking ring 603.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, the cell is conveniently assembled and sealed after being placed through the culture mechanism 1 and the sealing mechanism 2, so that the cell culture device is more convenient to assemble, and meanwhile, the monitoring mechanism can be better connected after the cell culture device is assembled, so that the change condition of culture liquid in the cell culture device can be better monitored in real time through the monitoring mechanism, and meanwhile, the cell culture device is more convenient and visual in observation, and the osmotic pressure and the PH value of the monitoring environment can be better displayed on the display screen 204 through real-time monitoring.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, the monitoring mechanism comprises the PH sensor 7 and the osmotic pressure detection member 5, the PH sensor 7 is fixedly arranged at the bottom end of the culture mechanism 1, and the osmotic pressure detection member 5 is fixedly arranged at the outer side of the culture mechanism 1.
In fig. 2, a cell culture apparatus capable of monitoring the liquid environment of cell culture is implemented, the monitoring mechanism mainly comprises a PH sensor 7 and an osmotic pressure detecting member 5, the PH sensor 7 is fixedly arranged at the bottom end inside the culture mechanism 1, the osmotic pressure detecting member 5 is fixedly arranged at the outer side of the culture mechanism 1, the osmotic pressure detecting member 5 and the PH sensor 7 are oppositely arranged, and the PH sensor 7 is preferably of a model of PH-5806.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, the culture liquid added into the culture mechanism 1 can be better monitored through the PH sensor 7 and the osmotic pressure detection member 5, the acid-base degree in the culture liquid and the osmotic pressure of the culture liquid can be better monitored, the environment during cell culture can be better determined, the growth condition of cells can be further judged through the PH value and the change of the osmotic pressure in the culture process, and the PH sensor 7 is preferably selected to be PH-5806 because of sensitive reaction speed, and the measured value is accurate.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, the top of the culture mechanism 1 adjacent to the PH sensor 7 and the osmotic pressure detection member 5 is provided with the communication port 10, the bottom end of the inside of the communication port 10 is fixedly provided with the clamping electric groove 101, and the clamping electric groove 101 is electrically connected with the PH sensor 7 and the osmotic pressure detection member 5.
It will be appreciated that: the osmotic pressure detecting member 5 includes a laser ranging sensor 504 and a reflecting sheet 503.
In fig. 2 and 6, a cell culture apparatus capable of monitoring a cell culture liquid environment is implemented, a communication port 10 is formed at the top of a culture mechanism 1 adjacent to a PH sensor 7 and an osmotic pressure detection member 5, a clamping electric tank 101 is fixedly arranged at the bottom end inside the communication port 10, the clamping electric tank 101 is electrically connected with the PH sensor 7 and the osmotic pressure detection member 5, and three clamping electric tanks 101 are preferably and uniformly arranged in the communication port 10.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, through the communication port 10 and the clamping electric groove 101 arranged in the communication port 10, when the sealing mechanism 2 is installed, the sealing mechanism 2 can be better fixed, meanwhile, the sealing mechanism 2 is conveniently communicated with the culture mechanism 1, and then, the clamping electric groove 101 is electrically connected with the PH sensor 7 and the osmotic pressure detection member 5, so that various data measured by the PH sensor 7 and the osmotic pressure detection member 5 are conveniently conveyed, and further, various data changes in the culture liquid are conveniently observed, and meanwhile, the clamping electric groove 101 is preferably arranged in the communication port 10, so that the PH sensor 7 and the osmotic pressure detection member 5 are conveniently electrically communicated, and meanwhile, the connection is more convenient.
According to the cell culture device capable of monitoring the cell culture liquid environment provided by the embodiment of the utility model, the clamping electric sheet 201 is fixedly arranged at the bottom of the sealing mechanism 2 corresponding to the communication port 10, and the clamping electric sheet 201 and the clamping electric groove 101 are electrically connected when the sealing mechanism 2 and the culture mechanism 1 are assembled.
In fig. 3 and 5, a cell culture device capable of monitoring a cell culture liquid environment is implemented, a clamping electric sheet 201 is fixedly arranged at the bottom of a sealing mechanism 2 corresponding to a communication port 10, meanwhile, the clamping electric sheet 201 and a clamping electric groove 101 are electrically connected when the sealing mechanism 2 and the culture mechanism 1 are assembled, three clamping electric sheets 201 corresponding to the clamping electric grooves 101 are preferably arranged, and the length of the clamping electric sheet 201 is identical to the depth of the communication port 10.
According to the cell culture device capable of monitoring the cell culture liquid environment, the clamping electric plates 201 are arranged, so that the culture mechanism 1 and the sealing mechanism 2 can be better embedded into the communication port 10 when being assembled, and then extend downwards after being embedded into the communication port 10, and further are fixedly clamped with the clamping electric grooves 101, so that the clamping electric grooves 101 are communicated with the clamping electric plates 201, namely, the monitoring mechanism is communicated, so that monitored data are transmitted, the clamping electric plates 201 are arranged to correspond to the clamping electric grooves 101 for better convenience in connection, poor contact is avoided, meanwhile, the length of the clamping electric plates 201 is set to be the same as the depth of the communication port 10, and the installation positions of the culture mechanism 1 and the sealing mechanism 2 are more compact, so that the gap is reduced, and meanwhile, the contact effect between the clamping electric plates 201 and the clamping electric grooves 101 is better.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, the top surface of the sealing mechanism 2 is fixedly provided with the display screen 204, one side of the display screen 204 is provided with the observation window 4, the inside of the sealing mechanism 2 is fixedly provided with the singlechip 203 and the controller 202, the singlechip 203 is electrically connected with the display screen 204, the controllers 202 are electrically arranged on two sides of the singlechip 203, and the two controllers 202 are electrically connected with the clamping electric sheet 201.
Fig. 4 and fig. 6 show a cell culture apparatus capable of monitoring a cell culture liquid environment, a display screen 204 is fixedly arranged on the top surface of a sealing mechanism 2, an observation window 4 is arranged on one side of the display screen 204, a single-chip microcomputer 203 and a controller 202 are arranged in the sealing mechanism 2, the single-chip microcomputer 203 is electrically connected with the display screen 204, the controller 202 is electrically arranged on two sides of the single-chip microcomputer 203, meanwhile, the two controllers 202 are electrically connected with a clamping electric sheet 201, the single-chip microcomputer 203 is preferably STM32, and data related to cell culture are downloaded and stored in the single-chip microcomputer 203 in advance through programming and downloading of a database, and meanwhile, each part is controlled through a programmed program.
According to the cell culture device capable of monitoring the cell culture liquid environment, the SCM 203 is electrically connected with the display screen 204 and the controller 202 through the SCM 203, so that data which are well analyzed and processed can be better displayed through the display screen 204, meanwhile, the controller 202 is controlled to work through the action of the SCM 203, and meanwhile, the controller 202 is enabled to conveniently control the PH sensor 7 and the osmotic pressure detection component 5 to work, so that real-time monitoring can be achieved, culture liquid does not need to be extracted and checked, culture experiment efficiency is improved, and the cell growth condition in the culture mechanism 1 can be better observed through the arranged observation window 4, and the SCM 203 is preferably STM32 because the STM32 SCM 203 is fast in operation speed, short in operation time, low in cost and the like.
According to the cell culture device capable of monitoring the cell culture liquid environment provided by the embodiment of the utility model, the liquid adding mechanism 6 comprises a liquid adding port 601 and a blanking ring 603, the liquid adding port 601 is formed in the top of the sealing mechanism 2, and the blanking ring 603 is fixedly arranged at the bottom of the sealing mechanism 2.
In fig. 2 and 3, a cell culture apparatus capable of monitoring a cell culture liquid environment is implemented, a liquid adding mechanism 6 is formed by combining a liquid adding port 601 and a blanking ring 603, the liquid adding port 601 is arranged at the top of a sealing mechanism 2, the blanking ring 603 is fixedly arranged at the bottom of the sealing mechanism 2, the liquid adding port 601 and the blanking ring 603 are communicated, meanwhile, the blanking ring 603 is preferably in a circular ring structure, and the blanking ring 603 is fixedly arranged between two clamping electric plates 201.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, the liquid adding port 601 is arranged at the top end of the sealing mechanism 2, and the blanking ring 603 is arranged at the bottom end of the sealing mechanism 2 through the liquid adding port 601 and the blanking ring 603, so that the liquid adding port 601 and the blanking ring 603 are communicated, further, culture liquid can be conveniently added into the culture mechanism 1 through the liquid adding port 601, further, the culture mechanism 1 does not need to be opened when the culture liquid is added, and further, the cells are better protected from being polluted and destroyed by external air.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, the top end of the inside of the liquid adding port 601 is fixedly provided with the sealing valve 602, the inside of the blanking ring 603 is provided with the liquid storage cavity 604, the lower part of the liquid storage cavity 604 penetrates through the bottom of the blanking ring 603 and is provided with the liquid outlet 605, and the liquid adding port is communicated with the liquid storage cavity 604.
Fig. 4 and 6 show a cell culture apparatus capable of monitoring a cell culture liquid environment, wherein a sealing flap 602 is fixedly arranged at the top end of the inside of a liquid adding port 601, a liquid storage cavity 604 is formed in the blanking ring 603, a liquid outlet 605 is formed below the liquid storage cavity 604 by penetrating through the bottom of the blanking ring 603, meanwhile, the sealing flap 602 is a flexible rubber elastic sheet, a plurality of combinations form a petal-shaped structure, the two are tightly contacted, and meanwhile, the liquid adding port 601 and the liquid storage cavity 604 are communicated.
According to the cell culture device capable of monitoring the cell culture liquid environment, the sealing valve 602 arranged in the liquid adding port 601 can better seal the liquid adding port 601 when culture liquid is not added, the sealing valve 602 is better used for closing the liquid adding port 601, so that the entry of external harmful substances is avoided, meanwhile, the liquid storage cavity 604 is arranged in the blanking ring 603, the added culture liquid can be better conveniently stored in the liquid storage cavity 604, the liquid outlet 605 is arranged at the bottom of the blanking ring 603 in a penetrating manner, so that the added culture liquid can be better and conveniently flowed out through the liquid outlet 605, flows into the culture mechanism 1, provides nutrition for cells, sets the sealing valve 602 as a flexible rubber elastic sheet, simultaneously, the sealing valve 602 is in a petal-shaped structure formed by combining a plurality of parts, is in close contact with each other, and further can conveniently jack the sealing valve 602 when the culture liquid is added, so that the culture liquid is convenient to add, and secondly, the sealing valve 602 is convenient to be automatically closed after the culture liquid is added, so that the pollution to cells when the culture liquid is added is more convenient to reduce.
According to the cell culture device capable of monitoring the cell culture liquid environment provided by the embodiment of the utility model, the osmotic pressure detection member 5 comprises the laser ranging sensor 504 and the reflecting plate 503, the detection cavity 501 is formed in the osmotic pressure detection member 5, the laser ranging sensor 504 is fixedly arranged at the top of the detection cavity 501, and the reflecting plate 503 is arranged below the detection cavity 501 corresponding to the laser ranging sensor 504.
In fig. 6 and 7, a cell culture apparatus capable of monitoring a cell culture liquid environment is implemented, an osmotic pressure detecting member 5 is formed by combining a laser ranging sensor 504 and a reflecting plate 503, a detecting cavity 501 is formed in the osmotic pressure detecting member 5, the laser ranging sensor 504 is fixedly arranged at the top of the detecting cavity 501, the reflecting plate 503 is arranged below the detecting cavity 501 corresponding to the laser ranging sensor 504, wherein the laser ranging sensor 504 is preferably of the type P1KY101, and the reflecting plate 503 is preferably a smooth reflecting surface.
According to the cell culture device capable of monitoring the cell culture liquid environment, the laser ranging sensor 504 and the reflecting sheet 503 are arranged in the osmotic pressure detection component 5, the laser emitted by the laser ranging sensor 504 irradiates the reflecting sheet 503, the laser is reflected to the laser ranging sensor 504 through the reflecting sheet 503, the distance between the laser sensor and the reflecting sheet 503 can be measured better, the reflecting sheet 503 is arranged in the liquid with the same concentration as the culture liquid, and when the concentrations on two sides are different, the water in low concentration can be absorbed, so that the water in low concentration is reduced, the concentration is increased, the reflecting sheet 503 floats upwards, and conversely, the reflecting sheet sinks, and the distance between the floating and sinking is measured, namely the change condition of osmotic pressure, so that the osmotic pressure is monitored more conveniently.
According to the cell culture device capable of monitoring the cell culture liquid environment provided by the embodiment of the utility model, the inner wall of the detection cavity 501 is provided with the sliding groove 502, and the reflecting sheet 503 is slidably arranged in the sliding groove 502.
In fig. 7, a cell culture apparatus capable of monitoring the liquid environment of cell culture is implemented, a sliding groove 502 is formed in the inner wall of a detection chamber 501, and a reflecting plate 503 is arranged in the sliding groove 502 in a demarcation manner, wherein the reflecting plate 503 may be rectangular or circular, and is preferably arranged as a circular mechanism.
According to the cell culture device capable of monitoring the cell culture liquid environment, the reflecting plate 503 can be better placed through the sliding groove 502, so that the reflecting plate 503 only moves in the vertical direction during movement, laser emitted by the laser ranging sensor 504 is better and conveniently reflected, ranging is better performed, and the reflecting plate 503 is arranged to be of a circular structure so as to better move in the detection cavity 501, so that movement is not hindered.
According to the cell culture device capable of monitoring the cell culture liquid environment provided by the embodiment of the utility model, the lower part of the osmotic pressure detection member 5 is communicated with the inside of the culture mechanism 1, and the semipermeable membrane 505 is fixedly arranged in the communicated inside.
In FIG. 7, a cell culture apparatus capable of monitoring the liquid environment of cell culture is shown, wherein the lower part of an osmotic pressure detecting member 5 is communicated with the inside of a culture mechanism 1, and a semipermeable membrane 505 is fixedly provided in the communicated inside.
According to the cell culture device capable of monitoring the cell culture liquid environment, provided by the embodiment of the utility model, through the semi-permeable membrane 505, only water flows when permeation occurs, so that mixed objects are not combined with each other, and meanwhile, loss of nutrient substances in the culture solution is prevented more conveniently, and further protection is provided.
The application method of the utility model comprises the following steps:
when the cell culture device is used, the inside of the culture mechanism 1 and the sealing mechanism 2 are disinfected and cleaned, then cells are placed into the culture mechanism 1 according to a culture flow of the cells to be cultured, then the culture mechanism 1 and the sealing mechanism 2 are assembled and sealed, in the process of assembling and sealing, the clamping electric plate 201 and the clamping electric groove 101 are connected, then the monitoring mechanism of the device is connected, then culture solution is added into the culture mechanism 1 through the liquid adding port 601, the cells are wrapped or paved on the bottom surface of the culture mechanism 1, then liquid with the same concentration is added into the detection cavity 501 in the osmotic pressure detection mechanism, then the singlechip 203 is connected with an external power supply, so that the singlechip 203, the controller 202, the osmotic pressure detection mechanism and the PH sensor 7 work, and then data of detection change are transmitted to the singlechip 203, the result and the data are displayed on the display screen 204 through the processing and analysis of the singlechip 203, and the change of the environment of the culture solution is observed more directly, the culture solution is not required to be extracted and the cell culture efficiency is improved better.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.

Claims (10)

1. A cell culture apparatus for monitoring the environment of a cell culture fluid, comprising:
a culture mechanism (1);
the sealing mechanism (2) is movably embedded on the culture mechanism (1);
the monitoring mechanism is fixedly arranged in the culture mechanism (1) and the sealing mechanism (2), and the monitoring mechanism is communicated through the mutual installation of the culture mechanism (1) and the sealing mechanism (2), so that the environment of the culture solution in the culture mechanism (1) is better monitored in real time;
and the liquid adding mechanism (6) is communicated with the sealing mechanism (2), and the liquid adding mechanism (6) can better add culture liquid into the culture mechanism (1) without opening the culture device.
2. The cell culture apparatus of claim 1, wherein the cell culture apparatus is configured to monitor an environment of a cell culture fluid: the monitoring mechanism comprises a PH sensor (7) and an osmotic pressure detection member (5), wherein the PH sensor (7) is fixedly arranged at the bottom end of the culture mechanism (1), and the osmotic pressure detection member (5) is fixedly arranged at the outer side of the culture mechanism (1).
3. A cell culture apparatus according to claim 2 wherein the cell culture fluid environment is monitored by: the PH sensor (7) and the osmotic pressure detection component (5) are adjacent, the top of the culture mechanism (1) is provided with a communication port (10), the bottom end of the inside of the communication port (10) is fixedly provided with a clamping electric groove (101), and the clamping electric groove (101) is electrically connected with the PH sensor (7) and the osmotic pressure detection component (5).
4. A cell culture apparatus according to claim 3 wherein the environment of the cell culture fluid is monitored: the bottom of sealing mechanism (2) corresponds intercommunication mouth (10) fixedly is provided with joint electric plate (201), joint electric plate (201) and joint electric tank (101) carry out the electric connection when sealing mechanism (2) and cultivation mechanism (1) assemble.
5. The cell culture apparatus of claim 4, wherein the cell culture apparatus is configured to monitor an environment of a cell culture fluid: the utility model discloses a sealing mechanism, including sealed mechanism (2), controller, clamping electric piece (201) and electric connection, the top surface of sealed mechanism (2) is fixed to be provided with display screen (204), one side of display screen (204) is provided with observation window (4), the inside of sealed mechanism (2) is fixed to be provided with singlechip (203) and controller (202), singlechip (203) carry out electric connection with display screen (204), controller (202) electric setting is in both sides of singlechip (203), two electric connection is carried out with clamping electric piece (201) to controller (202).
6. The cell culture apparatus of claim 1, wherein the cell culture apparatus is configured to monitor an environment of a cell culture fluid: the liquid adding mechanism (6) comprises a liquid adding port (601) and a blanking ring (603), the liquid adding port (601) is formed in the top of the sealing mechanism (2), and the blanking ring (603) is fixedly arranged at the bottom of the sealing mechanism (2).
7. The cell culture apparatus of claim 6, wherein the cell culture apparatus is configured to monitor an environment of a cell culture fluid: the inside top of adding liquid mouth (601) is fixed and is provided with sealed lamella (602), stock solution chamber (604) have been seted up to the inside of unloading ring (603), the below of stock solution chamber (604) is provided with liquid outlet (605) through unloading ring (603) bottom, it communicates with stock solution chamber (604) to add the material mouth.
8. A cell culture apparatus according to claim 3 wherein the environment of the cell culture fluid is monitored: the osmotic pressure detection component (5) comprises a laser ranging sensor (504) and a reflecting plate (503), a detection cavity (501) is formed in the osmotic pressure detection component (5), the laser ranging sensor (504) is fixedly arranged at the top of the detection cavity (501), and the reflecting plate (503) is arranged below the detection cavity (501) corresponding to the laser ranging sensor (504).
9. The cell culture apparatus of claim 8, wherein the cell culture apparatus is configured to monitor an environment of a cell culture fluid: the inner wall of the detection cavity (501) is provided with a sliding groove (502), and the reflecting plate (503) is arranged in the sliding groove (502) in a sliding way.
10. The cell culture apparatus of claim 8, wherein the cell culture apparatus is configured to monitor an environment of a cell culture fluid: the lower part of the osmotic pressure detection component (5) is communicated with the inside of the culture mechanism (1), and a semipermeable membrane (505) is fixedly arranged in the communicated inside.
CN202310610703.5A 2023-05-26 2023-05-26 Cell culture device capable of monitoring cell culture liquid environment Pending CN116694465A (en)

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Application Number Priority Date Filing Date Title
CN202310610703.5A CN116694465A (en) 2023-05-26 2023-05-26 Cell culture device capable of monitoring cell culture liquid environment

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Application Number Priority Date Filing Date Title
CN202310610703.5A CN116694465A (en) 2023-05-26 2023-05-26 Cell culture device capable of monitoring cell culture liquid environment

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CN116694465A true CN116694465A (en) 2023-09-05

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Application Number Title Priority Date Filing Date
CN202310610703.5A Pending CN116694465A (en) 2023-05-26 2023-05-26 Cell culture device capable of monitoring cell culture liquid environment

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Country Link
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117413773A (en) * 2023-10-26 2024-01-19 南京晓庄学院 Osmotic pressure differential pressure compensation control system for plant tissue culture and implementation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117413773A (en) * 2023-10-26 2024-01-19 南京晓庄学院 Osmotic pressure differential pressure compensation control system for plant tissue culture and implementation method thereof

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