CN116688244A - Antibacterial coating for central venous catheter and preparation method thereof - Google Patents
Antibacterial coating for central venous catheter and preparation method thereof Download PDFInfo
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- CN116688244A CN116688244A CN202310738014.2A CN202310738014A CN116688244A CN 116688244 A CN116688244 A CN 116688244A CN 202310738014 A CN202310738014 A CN 202310738014A CN 116688244 A CN116688244 A CN 116688244A
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 101
- 238000000576 coating method Methods 0.000 title claims abstract description 77
- 239000011248 coating agent Substances 0.000 title claims abstract description 75
- 238000002360 preparation method Methods 0.000 title description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000012046 mixed solvent Substances 0.000 claims abstract description 46
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 32
- WTJXVDPDEQKTCV-UHFFFAOYSA-N 4,7-bis(dimethylamino)-1,10,11,12a-tetrahydroxy-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide;hydron;chloride Chemical compound Cl.C1C2=C(N(C)C)C=CC(O)=C2C(O)=C2C1CC1C(N(C)C)C(=O)C(C(N)=O)=C(O)C1(O)C2=O WTJXVDPDEQKTCV-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229960002152 chlorhexidine acetate Drugs 0.000 claims abstract description 28
- 229960002421 minocycline hydrochloride Drugs 0.000 claims abstract description 28
- MCSINKKTEDDPNK-UHFFFAOYSA-N propyl propionate Chemical compound CCCOC(=O)CC MCSINKKTEDDPNK-UHFFFAOYSA-N 0.000 claims abstract description 28
- RDEIXVOBVLKYNT-VQBXQJRRSA-N (2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(1-aminoethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;(2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(aminomethyl)oxan-2-yl]o Chemical compound OS(O)(=O)=O.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@@H](CN)O2)N)[C@@H](N)C[C@H]1N.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@H](O2)C(C)N)N)[C@@H](N)C[C@H]1N.O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N RDEIXVOBVLKYNT-VQBXQJRRSA-N 0.000 claims abstract description 24
- HQYALQRYBUJWDH-UHFFFAOYSA-N trimethoxy(propyl)silane Chemical compound CCC[Si](OC)(OC)OC HQYALQRYBUJWDH-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000000203 mixture Substances 0.000 claims abstract description 13
- 239000003960 organic solvent Substances 0.000 claims abstract description 9
- 238000011010 flushing procedure Methods 0.000 claims description 38
- 239000008215 water for injection Substances 0.000 claims description 26
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 13
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 13
- 239000000741 silica gel Substances 0.000 claims description 13
- 229910002027 silica gel Inorganic materials 0.000 claims description 13
- 238000001816 cooling Methods 0.000 claims description 12
- 238000010790 dilution Methods 0.000 claims description 12
- 239000012895 dilution Substances 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 7
- 238000013007 heat curing Methods 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000007598 dipping method Methods 0.000 claims description 2
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 230000000845 anti-microbial effect Effects 0.000 claims 1
- 239000004599 antimicrobial Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 11
- 241000894006 Bacteria Species 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 5
- 238000013329 compounding Methods 0.000 abstract description 4
- 230000000295 complement effect Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 238000001723 curing Methods 0.000 description 10
- BPSIOYPQMFLKFR-UHFFFAOYSA-N trimethoxy-[3-(oxiran-2-ylmethoxy)propyl]silane Chemical compound CO[Si](OC)(OC)CCCOCC1CO1 BPSIOYPQMFLKFR-UHFFFAOYSA-N 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 6
- 229930182566 Gentamicin Natural products 0.000 description 4
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000222122 Candida albicans Species 0.000 description 2
- 241000192125 Firmicutes Species 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 208000004429 Bacillary Dysentery Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 229940123208 Biguanide Drugs 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010017915 Gastroenteritis shigella Diseases 0.000 description 1
- 206010022678 Intestinal infections Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000006087 Silane Coupling Agent Substances 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 229940126574 aminoglycoside antibiotic Drugs 0.000 description 1
- 239000002647 aminoglycoside antibiotic agent Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- -1 biguanide compound Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009530 blood pressure measurement Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000001631 haemodialysis Methods 0.000 description 1
- 230000000322 hemodialysis Effects 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 208000001297 phlebitis Diseases 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007142 ring opening reaction Methods 0.000 description 1
- 201000005113 shigellosis Diseases 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 229940072172 tetracycline antibiotic Drugs 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight: 0.1-5 parts of gentamicin sulfate, 0.1-5 parts of chlorhexidine acetate, 0.2-3 parts of minocycline hydrochloride, 8-18.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 75-85 parts of mixed solvent; the mixed solvent is a mixture of an organic solvent and water, the organic solvent is methanol or ethanol, and the mass ratio of the organic solvent to the water is 0.5:1 to 9:1, the broad-spectrum antibacterial agent containing specific components is prepared by compounding three medicines with strong antibacterial property into the antibacterial coating for the central venous catheter, so that the mutual conflict of medicine effects is avoided, the medicine effects of the three medicines complement each other to generate a synergistic effect by limiting the compounding proportion, the efficacy is more obvious than that of a single component, and the optimal antibacterial proportion is obtained through experiments, so that various bacteria can be effectively inhibited, and the antibacterial effect is excellent.
Description
Technical Field
The invention relates to the technical field of biological medicine, in particular to an antibacterial coating for a central venous catheter and a preparation method thereof.
Background
Minocycline hydrochloride is a tetracycline broad-spectrum antibiotic, has the strongest antibacterial effect in the tetracycline antibiotic, can inhibit the synthesis of bacteria or other pathogenic microorganism proteins, thereby playing the role of bacteriostasis and sterilization, and is used as a broad-spectrum antibiotic singly; the chlorhexidine acetate is a biguanide compound, has quite strong broad-spectrum antibacterial and bactericidal effects, is effective to gram-positive bacteria and gram-negative bacteria, and is not easy to generate drug resistance after long-term use; the gentamicin sulfate is an aminoglycoside antibiotic, has good antibacterial effect on various gram-negative bacteria and gram-positive bacteria, has a relatively wide antibacterial spectrum, and is mainly used for treating bacillary dysentery or other bacterial intestinal infections;
the central venous catheter is generally made of silica gel materials, is used as an indispensable tool in clinical treatment work, has been widely applied to the diagnosis and treatment of diseases such as central venous pressure measurement, intravenous transfusion, hemodialysis, tumor chemotherapy and the like, and is especially used frequently for patients who input blood and medicines repeatedly, and needs to be left in the human body for one week, two weeks or even longer. The problem of easy occurrence of bacterial infection after long-term use increases the risks of venous infection and phlebitis, thereby generating medical accidents and seriously endangering the life safety of patients. At present, most of antibacterial central venous catheters have the problems that an antibacterial coating is easy to fall off, the antibacterial performance is unstable, the duration of an antibacterial effect is short, the antibacterial effect is poor, and the like.
Disclosure of Invention
In order to solve the problems, the invention provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
0.1-5 parts of gentamicin sulfate, 0.1-5 parts of chlorhexidine acetate, 0.2-3 parts of minocycline hydrochloride, 8-18.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 75-85 parts of mixed solvent;
the mixed solvent is a mixture of an organic solvent and water; the organic solvent is methanol or ethanol, wherein the mass ratio of the organic solvent to water is 0.5:1 to 9:1.
the preparation method of the antibacterial coating for the central venous catheter comprises the following steps of:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for a plurality of times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Irradiating under a Y-ray source of Co;
preparing a mixed solvent, and mixing methanol or ethanol with water according to a mass ratio of 0.5:1 to 9:1, mixing to obtain methanol water or ethanol water;
placing 0.1-5 parts of gentamicin sulfate, 0.1-5 parts of chlorhexidine acetate, 0.2-3 parts of minocycline hydrochloride and 8-18.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 75-85 parts of the mixed solvent for dilution, and stirring until the mixed solvent is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 2-24h, taking out, flushing with water for injection for several times, heating and solidifying the coating, and standing and cooling to obtain the finished product.
Further, the mixed solvent is methanol water, and the mass ratio is 4:1 methanol is mixed with water.
Further, the mixed solvent is ethanol water, and the mass ratio is 6:1 ethanol is mixed with water.
Further, the irradiation rate is 4-8kGy/h, and the irradiation dose is 40-60kGy.
Further, the irradiation rate was 6kGy/h and the irradiation dose was 50kGy.
Further, the dipping time was 12 hours.
Further, the number of times of flushing the central venous catheter with water for injection was 10 times each.
Further, the heating curing temperature is 40-120 ℃, and the heating curing time is 0.5-5h.
Further, the heat curing temperature was 80℃and the heat curing time was 2.5 hours.
The beneficial effects are that:
(1) The antibacterial coating for the central venous catheter provided by the invention contains the broad-spectrum antibacterial agent with specific components, and three medicaments with strong antibacterial property are compounded into the antibacterial coating for the central venous catheter, so that the mutual conflict of medicament effects is avoided, the medicament effects of the three medicaments complement each other to generate a synergistic effect by limiting the compounding proportion, the effects are more obvious than those of a single component, and the optimal antibacterial proportion is obtained through experiments, so that various bacteria can be effectively inhibited, and the antibacterial effect is excellent.
(2) The antibacterial coating for the central venous catheter is prepared by combining three medicaments with strong antibacterial property through radiation treatment of the central venous catheter by using y rays, so that the antibacterial coating is more difficult to fall off, the duration of the antibacterial effect is longer, the antibacterial performance is more stable, and the antibacterial effect is better.
(3) The preparation method of the antibacterial coating for the central venous catheter provided by the invention is simple to operate, convenient to use, short in production period and beneficial to popularization and application.
Detailed Description
The present invention is described in detail below with reference to examples to facilitate understanding of the present invention by those skilled in the art. It is specifically pointed out that the examples are given solely for the purpose of illustration of the invention and are not to be construed as limiting the scope of the invention, since numerous insubstantial modifications and variations of the invention will be within the scope of the invention, as described above, will become apparent to those skilled in the art. Meanwhile, the raw materials mentioned below are not specified, and are all commercial products; the process steps or preparation methods not mentioned in detail are those known to the person skilled in the art.
Example 1
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
3 parts of gentamicin sulfate, 3 parts of chlorhexidine acetate, 3 parts of minocycline hydrochloride, 16 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 75 parts of methanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 5 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 4kGy/h, and the irradiation dose is 40kGy;
preparing a mixed solvent, and mixing methanol and water according to a mass ratio of 0.5:1, mixing to obtain methanol water;
placing 3 parts of gentamicin sulfate, 3 parts of chlorhexidine acetate, 3 parts of minocycline hydrochloride and 16 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 75 parts of methanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 2 hours, taking out, flushing for 5 times by using water for injection, heating and solidifying the coating at the temperature of 40 ℃ for 0.5 hour, and standing and cooling to obtain a finished product.
Example two
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
0.1 part of gentamicin sulfate, 5 parts of chlorhexidine acetate, 1.4 parts of minocycline hydrochloride, 18.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 75 parts of ethanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 5 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 4kGy/h, and the irradiation dose is 40kGy;
preparing a mixed solvent, namely mixing ethanol with water according to a mass ratio of 0.5:1, mixing to obtain ethanol water;
placing 0.1 part of gentamicin sulfate, 5 parts of chlorhexidine acetate, 1.4 parts of minocycline hydrochloride and 18.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 75 parts of ethanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 2 hours, taking out, flushing for 5 times by using water for injection, heating and solidifying the coating at the temperature of 40 ℃ for 0.5 hour, and standing and cooling to obtain a finished product.
Example III
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
5 parts of gentamicin sulfate, 0.1 part of chlorhexidine acetate, 2.4 parts of minocycline hydrochloride, 12.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 80 parts of methanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 5 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 6kGy/h, and the irradiation dose is 50kGy;
preparing a mixed solvent, namely mixing methanol with water according to a mass ratio of 4:1, mixing to obtain methanol water;
placing 5 parts of gentamicin sulfate, 0.1 part of chlorhexidine acetate, 2.4 parts of minocycline hydrochloride and 12.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 80 parts of methanol-water mixed solvent for dilution, and stirring until the components are completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 12 hours, taking out, flushing for 10 times by using water for injection, heating and solidifying the coating at the temperature of 80 ℃ for 2.5 hours, and standing and cooling to obtain a finished product.
Example IV
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
4 parts of gentamicin sulfate, 2 parts of chlorhexidine acetate, 0.2 part of minocycline hydrochloride, 13.8 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 80 parts of ethanol water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 10 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 6kGy/h, and the irradiation dose is 50kGy;
preparing a mixed solvent, namely mixing ethanol with water according to a mass ratio of 6:1, mixing to obtain ethanol water;
placing 4 parts of gentamicin sulfate, 2 parts of chlorhexidine acetate, 0.2 part of minocycline hydrochloride and 13.8 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 80 parts of ethanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 12 hours, taking out, flushing for 10 times by using water for injection, heating and solidifying the coating at the temperature of 80 ℃ for 2.5 hours, and standing and cooling to obtain a finished product.
Example five
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
1 part of gentamicin sulfate, 3 parts of chlorhexidine acetate, 1 part of minocycline hydrochloride, 10 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 85 parts of methanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 15 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 8kGy/h, and the irradiation dose is 60kGy;
preparing a mixed solvent, namely mixing methanol with water according to a mass ratio of 9:1, mixing to obtain methanol water;
placing 1 part of gentamicin sulfate, 3 parts of chlorhexidine acetate, 1 part of minocycline hydrochloride and 10 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 85 parts of methanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 24 hours, taking out, flushing with water for injection for 15 times, heating and solidifying the coating at 120 ℃ for 5 hours, and standing and cooling to obtain the finished product.
Example six
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
2.8 parts of gentamicin sulfate, 4 parts of chlorhexidine acetate, 0.2 part of minocycline hydrochloride, 8 parts of gamma- (2, 3-epoxypropoxy) propyl trimethoxysilane KH560 and 85 parts of ethanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 15 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 8kGy/h, and the irradiation dose is 60kGy;
preparing a mixed solvent, namely mixing ethanol with water according to a mass ratio of 9:1, mixing to obtain ethanol water;
placing 2.8 parts of gentamicin sulfate, 4 parts of chlorhexidine acetate, 0.2 part of minocycline hydrochloride and 8 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 85 parts of ethanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 24 hours, taking out, flushing with water for injection for 15 times, heating and solidifying the coating at 120 ℃ for 5 hours, and standing and cooling to obtain the finished product.
Comparative example one
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
7.5 parts of gentamicin sulfate, 12.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 80 parts of methanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 10 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 6kGy/h, and the irradiation dose is 50kGy;
preparing a mixed solvent, namely mixing methanol with water according to a mass ratio of 4:1, mixing to obtain methanol water;
placing 7.5 parts of gentamicin sulfate and 12.5 parts of gamma- (2, 3-epoxypropoxy) propyl trimethoxy silane KH560 into a glass container, adding 80 parts of methanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 12 hours, taking out, flushing for 10 times by using water for injection, heating and solidifying the coating at the temperature of 80 ℃ for 2.5 hours, and standing and cooling to obtain a finished product.
Comparative example two
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
7.5 parts of chlorhexidine acetate, 12.5 parts of gamma- (2, 3-epoxypropoxy) propyl trimethoxy silane KH560 and 80 parts of methanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 10 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 6kGy/h, and the irradiation dose is 50kGy;
preparing a mixed solvent, namely mixing methanol with water according to a mass ratio of 4:1, mixing to obtain methanol water;
placing 7.5 parts of chlorhexidine acetate and 12.5 parts of gamma- (2, 3-epoxypropoxy) propyl trimethoxy silane KH560 into a glass container, adding 80 parts of methanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 12 hours, taking out, flushing for 10 times by using water for injection, heating and solidifying the coating at the temperature of 80 ℃ for 2.5 hours, and standing and cooling to obtain a finished product.
Comparative example three
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
7.5 parts of minocycline hydrochloride, 12.5 parts of gamma- (2, 3-epoxypropoxy) propyl trimethoxysilane KH560 and 80 parts of methanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 10 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 6kGy/h, and the irradiation dose is 50kGy;
preparing a mixed solvent, namely mixing methanol with water according to a mass ratio of 4:1, mixing to obtain methanol water;
placing 7.5 parts of minocycline hydrochloride and 12.5 parts of gamma- (2, 3-epoxypropoxy) propyl trimethoxy silane KH560 into a glass container, adding 80 parts of methanol-water mixed solvent for dilution, and stirring until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 12 hours, taking out, flushing for 10 times by using water for injection, heating and solidifying the coating at the temperature of 80 ℃ for 2.5 hours, and standing and cooling to obtain a finished product.
Comparative example four
The embodiment provides an antibacterial coating for a central venous catheter, which comprises the following components in parts by weight:
20 parts of gamma- (2, 3-epoxypropoxy) propyl trimethoxy silane KH560 and 80 parts of methanol-water mixed solvent;
the preparation method comprises the following steps:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for 10 times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Carrying out irradiation under a Co y-ray source, wherein the irradiation rate is 6kGy/h, and the irradiation dose is 50kGy;
preparing a mixed solvent, namely mixing methanol with water according to a mass ratio of 4:1, mixing to obtain methanol water;
20 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 is placed in a glass container, 80 parts of methanol-water mixed solvent is added for dilution, and the mixture is stirred until the mixture is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 12 hours, taking out, flushing for 10 times by using water for injection, heating and solidifying the coating at the temperature of 80 ℃ for 2.5 hours, and standing and cooling to obtain a finished product.
Antibacterial effect study
1.1 materials and samples
Test strain: coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and bacillus viridis.
1.2 Experimental methods
Antibacterial coatings for central venous catheters obtained in examples 1 to 6 and comparative examples 1 to 4 were subjected to antibacterial tests using a bacteriostasis ring test method, respectively: the central venous catheter is vertically inserted into a culture medium flat plate at a small section (5 mm), the culture is carried out for 24 hours, the diameter of the inhibition ring is measured by a vernier caliper, the larger the diameter of the inhibition ring is, the better the antibacterial effect is, and the test result is shown in table 1.
1.3 experimental results
Table 1 shows the results of antibacterial tests of antibacterial coatings for central venous catheters prepared in examples 1 to 6 and comparative examples 1 to 4 according to the present invention.
Table 1 test results
From the data in table 1, it can be seen that:
(1) The antibacterial coating for the central venous catheter, which is prepared by the invention, has the antibacterial effect that the antibacterial effect of the antibacterial coating for the central venous catheter, which is prepared by the invention, can be maintained for at least 44 days, compared with the antibacterial effect in the prior art, the duration time is longer, and the antibacterial performance is more stable, because the antibacterial coating for the central venous catheter, which is prepared by the invention, breaks the C-H bond on the surface of the central venous catheter by using y rays to radiate the central venous catheter, so that the C-H bond on the surface of the central venous catheter is broken to release H atoms, the surface activity of the central venous catheter is improved, gamma- (2, 3-epoxypropoxy) propyl trimethoxysilane KH560 is a common silane coupling agent, the KH560 can be grafted with the surface of the activated central venous catheter, the end groups of minocycline hydrochloride, chlorhexidine acetate and gentamicin sulfate are respectively provided with amino groups with stronger activity in a chemical bonding mode, and the end groups of the minocycline hydrochloride, the chlorhexidine acetate and gentamicin sulfate are grafted onto the KH560 by using the ring-opening reaction of the amino groups and the epoxy groups, the bonding force between the KH560 and the surface of the central venous catheter is improved, and the antibacterial coating is remarkably improved, and the antibacterial bonding force between the surface of the active layer and the central venous catheter and the surface of the central venous catheter is improved;
(2) The antibacterial effect of the embodiment 1 to the embodiment 6 on five test strains, namely escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and bacillus viridis is, wherein the antibacterial effect of the embodiment 3 is optimal, which means that different proportions among the components have great influence on the antibacterial effect, for example, the polarity of a mixed reagent can influence the solubility of gentamicin sulfate, chlorhexidine acetate and minocycline hydrochloride so as to influence the antibacterial effect; the ratio of KH560 also affects the combination ratio of minocycline hydrochloride, chlorhexidine acetate and gentamycin sulfate with the central venous catheter so as to affect the antibacterial effect, the ratio of KH560 is too small, the combination ratio of minocycline hydrochloride, chlorhexidine acetate and gentamycin sulfate with the central venous catheter is reduced, the ratio of KH560 is too high, the waste of KH560 is caused, and the combination ratio of minocycline hydrochloride, chlorhexidine acetate and gentamycin sulfate with the central venous catheter is not improved; in addition, in the radiation treatment of the central venous catheter, the radiation rate and the radiation dose can damage the C-H bond of the central venous catheter to different degrees so as to influence the antibacterial effect; finally, the combination rate of minocycline hydrochloride, chlorhexidine acetate and gentamycin sulfate with the central venous catheter can be influenced by the immersion time, the heating curing temperature and the heating curing time, and the combination rate is higher when the immersion time and the heating curing time are longer in a certain range; the soaking time and the heating curing time are long, so that the minocycline hydrochloride, the chlorhexidine acetate and the gentamicin sulfate can be combined with the central venous catheter for curing, but the central venous catheter is damaged due to overlong time; too low a heating curing temperature may not achieve the curing effect or too high a heating curing temperature may damage the central venous catheter;
(3) Under the same time period, the data of examples 1-6 and comparative examples 1-4 show that three medicines with strong antibacterial property are compounded into the antibacterial coating for the central venous catheter, so that the mutual conflict of medicine effects is avoided, the medicine effects of the three medicines complement each other to generate a synergistic effect due to the limitation of the compounding proportion, and the antibacterial coating has more remarkable effects, wider antibacterial range and longer antibacterial time than the single component.
The above is merely an embodiment of the present invention, and is not intended to limit the present invention. Various modifications and variations of the present invention will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, or the like, which is within the spirit and principles of the present invention, should be included in the scope of the claims of the present invention.
Claims (10)
1. The antibacterial coating for the central venous catheter is characterized by comprising the following components in parts by weight:
0.1-5 parts of gentamicin sulfate, 0.1-5 parts of chlorhexidine acetate, 0.2-3 parts of minocycline hydrochloride, 8-18.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 and 75-85 parts of mixed solvent;
the mixed solvent is a mixture of an organic solvent and water; the organic solvent is methanol or ethanol, wherein the mass ratio of the organic solvent to water is 0.5:1 to 9:1.
2. a method of preparing an antimicrobial coating for a central venous catheter as claimed in claim 1, comprising the steps of:
taking out the central venous catheter made of silica gel material, flushing the central venous catheter for a plurality of times by water for injection, flushing the inner surface and the outer surface of the catheter by medical alcohol, and airing;
taking out the dried central venous catheter and placing the central venous catheter in 60 Irradiating under a Y-ray source of Co;
preparing a mixed solvent, and mixing methanol or ethanol with water according to a mass ratio of 0.5:1 to 9:1, mixing to obtain methanol water or ethanol water;
placing 0.1-5 parts of gentamicin sulfate, 0.1-5 parts of chlorhexidine acetate, 0.2-3 parts of minocycline hydrochloride and 8-18.5 parts of gamma- (2, 3-glycidoxy) propyl trimethoxy silane KH560 into a glass container, adding 75-85 parts of the mixed solvent for dilution, and stirring until the mixed solvent is completely dissolved to prepare an antibacterial coating;
immersing the irradiated central venous catheter in the antibacterial coating for 2-24h, taking out, flushing with water for injection for several times, heating and solidifying the coating, and standing and cooling to obtain the finished product.
3. The method for preparing the antibacterial coating for the central venous catheter according to claim 2, wherein the mixed solvent is methanol water, and the mass ratio is 4:1 methanol is mixed with water.
4. The method for preparing the antibacterial coating for the central venous catheter according to claim 2, wherein the mixed solvent is ethanol water, and the mass ratio is 6:1 ethanol is mixed with water.
5. The method for producing an antibacterial coating for a central venous catheter according to claim 2, characterized in that the irradiation rate is 4-8kGy/h and the irradiation dose is 40-60kGy.
6. The method for producing an antibacterial coating for a central venous catheter according to claim 2, characterized in that the irradiation rate is 6kGy/h and the irradiation dose is 50kGy.
7. The method for preparing an antibacterial coating for a central venous catheter according to claim 2, wherein the dipping time is 12 hours.
8. The method for producing an antibacterial coating for a central venous catheter according to claim 2, wherein the number of times of flushing the central venous catheter with water for injection is 10 times each.
9. The method for preparing an antibacterial coating for a central venous catheter according to claim 2, wherein the heat curing temperature is 40-120 ℃ and the heat curing time is 0.5-5h.
10. The method for preparing an antibacterial coating for a central venous catheter according to claim 2, wherein the heat curing temperature is 80 ℃ and the heat curing time is 2.5h.
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