CN116675583B - Green efficient soil phosphate fertilizer synergist and preparation method thereof - Google Patents
Green efficient soil phosphate fertilizer synergist and preparation method thereof Download PDFInfo
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- CN116675583B CN116675583B CN202310718553.XA CN202310718553A CN116675583B CN 116675583 B CN116675583 B CN 116675583B CN 202310718553 A CN202310718553 A CN 202310718553A CN 116675583 B CN116675583 B CN 116675583B
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- 239000002689 soil Substances 0.000 title claims abstract description 76
- 239000002686 phosphate fertilizer Substances 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 71
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 66
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 66
- 241000018646 Pinus brutia Species 0.000 claims abstract description 66
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 57
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims abstract description 54
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000011574 phosphorus Substances 0.000 claims abstract description 54
- 230000000813 microbial effect Effects 0.000 claims abstract description 35
- 241000223261 Trichoderma viride Species 0.000 claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 32
- 230000004151 fermentation Effects 0.000 claims abstract description 32
- 239000002994 raw material Substances 0.000 claims abstract description 28
- 210000003608 fece Anatomy 0.000 claims abstract description 25
- 239000010871 livestock manure Substances 0.000 claims abstract description 24
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 23
- 239000002738 chelating agent Substances 0.000 claims abstract description 22
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 22
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 22
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 20
- 239000004021 humic acid Substances 0.000 claims abstract description 20
- 235000006408 oxalic acid Nutrition 0.000 claims abstract description 18
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 18
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000004202 carbamide Substances 0.000 claims abstract description 17
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims description 55
- 238000012258 culturing Methods 0.000 claims description 26
- 239000003337 fertilizer Substances 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 20
- 239000000758 substrate Substances 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 8
- 229920002472 Starch Polymers 0.000 claims description 7
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 7
- 239000008107 starch Substances 0.000 claims description 7
- 235000019698 starch Nutrition 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- 229940061605 tetrasodium glutamate diacetate Drugs 0.000 claims description 6
- UZVUJVFQFNHRSY-OUTKXMMCSA-J tetrasodium;(2s)-2-[bis(carboxylatomethyl)amino]pentanedioate Chemical group [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CC[C@@H](C([O-])=O)N(CC([O-])=O)CC([O-])=O UZVUJVFQFNHRSY-OUTKXMMCSA-J 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000005469 granulation Methods 0.000 claims description 5
- 230000003179 granulation Effects 0.000 claims description 5
- 239000002985 plastic film Substances 0.000 claims description 5
- 229920006255 plastic film Polymers 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 238000005096 rolling process Methods 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- 238000003892 spreading Methods 0.000 claims description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 7
- 229910021645 metal ion Inorganic materials 0.000 abstract description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 5
- 239000011591 potassium Substances 0.000 abstract description 5
- 229910052700 potassium Inorganic materials 0.000 abstract description 5
- 238000004090 dissolution Methods 0.000 abstract description 4
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 17
- 230000002906 microbiologic effect Effects 0.000 description 15
- 239000011575 calcium Substances 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 11
- 240000008042 Zea mays Species 0.000 description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 7
- 235000005822 corn Nutrition 0.000 description 7
- 239000012747 synergistic agent Substances 0.000 description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- 229910019142 PO4 Inorganic materials 0.000 description 6
- 229910052791 calcium Inorganic materials 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000004720 fertilization Effects 0.000 description 4
- 239000002367 phosphate rock Substances 0.000 description 4
- OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 3
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000019837 monoammonium phosphate Nutrition 0.000 description 3
- 239000006012 monoammonium phosphate Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- -1 Ca 2 -P Chemical class 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108010020346 Polyglutamic Acid Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 229920002643 polyglutamic acid Polymers 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- VWVRASTUFJRTHW-UHFFFAOYSA-N 2-[3-(azetidin-3-yloxy)-4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]pyrazol-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound O=C(CN1C=C(C(OC2CNC2)=N1)C1=CN=C(NC2CC3=C(C2)C=CC=C3)N=C1)N1CCC2=C(C1)N=NN2 VWVRASTUFJRTHW-UHFFFAOYSA-N 0.000 description 1
- SXAMGRAIZSSWIH-UHFFFAOYSA-N 2-[3-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-1,2,4-oxadiazol-5-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1=NOC(=N1)CC(=O)N1CC2=C(CC1)NN=N2 SXAMGRAIZSSWIH-UHFFFAOYSA-N 0.000 description 1
- XXZCIYUJYUESMD-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-3-(morpholin-4-ylmethyl)pyrazol-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C=1C(=NN(C=1)CC(=O)N1CC2=C(CC1)NN=N2)CN1CCOCC1 XXZCIYUJYUESMD-UHFFFAOYSA-N 0.000 description 1
- LPZOCVVDSHQFST-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-3-ethylpyrazol-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C=1C(=NN(C=1)CC(=O)N1CC2=C(CC1)NN=N2)CC LPZOCVVDSHQFST-UHFFFAOYSA-N 0.000 description 1
- WZFUQSJFWNHZHM-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)N1CC2=C(CC1)NN=N2 WZFUQSJFWNHZHM-UHFFFAOYSA-N 0.000 description 1
- JVKRKMWZYMKVTQ-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]pyrazol-1-yl]-N-(2-oxo-3H-1,3-benzoxazol-6-yl)acetamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C=1C=NN(C=1)CC(=O)NC1=CC2=C(NC(O2)=O)C=C1 JVKRKMWZYMKVTQ-UHFFFAOYSA-N 0.000 description 1
- YJLUBHOZZTYQIP-UHFFFAOYSA-N 2-[5-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-1,3,4-oxadiazol-2-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1=NN=C(O1)CC(=O)N1CC2=C(CC1)NN=N2 YJLUBHOZZTYQIP-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- JAQXDZTWVWLKGC-UHFFFAOYSA-N [O-2].[Al+3].[Fe+2] Chemical compound [O-2].[Al+3].[Fe+2] JAQXDZTWVWLKGC-UHFFFAOYSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical class O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 150000001923 cyclic compounds Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical class [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 108010040003 polyglutamine Proteins 0.000 description 1
- 229920000155 polyglutamine Polymers 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical class [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a green efficient soil phosphate fertilizer synergist and a preparation method thereof, belonging to the technical field of microbial fermentation. The invention relates to a green efficient soil phosphate fertilizer synergist which consists of a regulator, animal manure, a chelating agent, a microbial agent, oxalic acid, humic acid, a water-retaining agent, urea and monopotassium phosphate. The invention uses pine needles as raw materials to prepare the phosphate fertilizer synergist, and the raw materials are used as waste resources; the added trichoderma viride, aspergillus niger and bacillus megaterium are synergistic, so that the dissolution of insoluble phosphorus is obviously promoted; the water-retaining agent is added in the preparation process to play a role in retaining water, and potassium dihydrogen phosphate and urea are added to introduce potassium and nitrogen elements; humic acid, sulfur powder and animal manure improve soil, chelating agent combines metal ions, oxalic acid dissolves insoluble calcium carbonate, the content of soluble phosphorus in the soil is greatly improved, the raw materials are environment-friendly, pollution is avoided, and the method is convenient to use and suitable for wide application.
Description
Technical Field
The invention belongs to the field of microbial fermentation, and particularly relates to a green efficient soil phosphate fertilizer synergist and a preparation method thereof.
Background
The phosphorite resources in China are lack, the high-grade phosphorite is few, the low-grade phosphorite is scattered, and the phosphorite is difficult to develop and utilize. The use of phosphate fertilizer has the problems that the fertilizer application amount is large, nutrients cannot be fully absorbed, the utilization rate is low, and the waste of resources and the pollution to the environment are caused.
The reason for low phosphate fertilizer utilization rate is mainly that (1) soluble phosphate ions in phosphate fertilizer can be complexed with metal ions such as iron, aluminum, calcium, magnesium and the like in soil to form insoluble phosphate which is difficult to be utilized, and (2) mobility of phosphorus is poor, and a large amount of applied phosphate fertilizer can be absorbed by soil to be converted into insoluble substances.
The existing phosphate fertilizer synergistic agent used at home and abroad is mainly considered from two aspects, wherein the first aspect is to add synergistic substances into phosphorus to react with the phosphorus so as to increase the solubility of the phosphorus; on the other hand, the physicochemical properties of the soil are changed so as to reduce the fixation of the phosphate fertilizer. Aiming at the application of a phosphate fertilizer synergist, humic acid or polyglutamic acid is added into the fertilizer synergist, and a large amount of active functional groups in the humic acid have stronger complexing, chelating and surface adsorption effects, so that the movement of the phosphate fertilizer is increased; the polyglutamine has extremely strong chelating ability to cations, competes with phosphate ions, reduces the combination of phosphate and metal ions, and increases the absorption of effective phosphorus of plants.
At present, humic acid and polyglutamic acid are added into many phosphate fertilizer synergists, but the utilization rate of the phosphate fertilizer is still very low, and the effect of increasing the utilization rate of the phosphate fertilizer serving as the phosphate fertilizer synergist is not obvious. The phosphate fertilizer is used as one of three major elements for plant growth, participates in photosynthesis and biochemistry of plants, and plays an important role in promoting plant growth and development and metabolism. Therefore, the green efficient phosphate fertilizer synergist provided by the invention solves the problems of phosphate fertilizer solidification, improves the fertilizer utilization rate, reduces the phosphate fertilizer usage amount, and is very necessary for alleviating agricultural pollution brought by chemical fertilizers in the aspect of crop growth and fertilization.
Disclosure of Invention
The invention overcomes the defects of the prior art and discloses a green efficient soil phosphate fertilizer synergist and a preparation method thereof. The invention relates to a green efficient soil phosphate fertilizer synergist which consists of a regulator, animal manure, a chelating agent, a microbial agent, oxalic acid, humic acid, a water-retaining agent, urea and monopotassium phosphate. The phosphorus fertilizer synergist prepared by taking pine needles as raw materials is utilized as raw material waste resources; the added trichoderma viride, aspergillus niger and bacillus megaterium are synergistic, so that the dissolution of insoluble phosphorus is obviously promoted; the water-retaining agent is added in the preparation process to play a role in retaining water, and potassium dihydrogen phosphate and urea are added to introduce potassium and nitrogen elements; humic acid, sulfur powder and animal manure improve soil, chelating agent combines metal ions, oxalic acid dissolves insoluble calcium carbonate, and the content of soluble phosphorus in the soil is greatly improved.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
the green efficient soil phosphate fertilizer synergist comprises, by weight, 40-65 parts of a regulator, 20-100 parts of animal manure, 1-5 parts of a chelating agent, 2-6 parts of a microbial agent, 30-50 parts of oxalic acid, 10-30 parts of humic acid, 2-5 parts of a water retaining agent, 15-20 parts of urea and 5-8 parts of potassium dihydrogen phosphate.
Preferably, the regulator is pine needle and sulfur powder with the mass ratio of 50:1.
More preferably, the pine needles include pine needles, pine stumps, and pine litter.
Preferably, the chelating agent is tetrasodium glutamate diacetate.
Preferably, the microbial agents are trichoderma viride, aspergillus niger and bacillus megaterium, and the volume ratio is 1:1:1.
more preferably, the trichoderma viride is purchased from the China General Microbiological Collection Center (CGMCC), the address is the number 3 of the North West road in the Korean area of Beijing, the preservation number is CGMCC3.15484, the aspergillus niger is purchased from the China General Microbiological Collection Center (CGMCC), the address is the number 3 of the North West road in the Korean area of Beijing, the preservation number is CGMCC3.15663, the bacillus megaterium is purchased from the China General Microbiological Collection Center (CGMCC), the address is the number 3 of the North West road in the Korean area of Beijing, and the preservation number is CGMCC1.16094.
Preferably, the water-retaining agent is carboxymethyl starch.
The preparation method of the green efficient soil phosphate fertilizer synergist comprises the following steps:
(1) And (3) preparing a microbial agent:
culturing Trichoderma viride, aspergillus niger and Bacillus megaterium in seed culture medium at 25deg.C, 25deg.C and 30deg.C for 24-48 hr, inoculating LB culture medium at 8% inoculum size, shake culturing at 25deg.C and 30deg.C to a bacterial concentration OD600 about 3.0, and culturing at 1:1:1, uniformly mixing the components in a volume ratio, and freeze-drying the components to obtain freeze-dried powder to obtain a microbial agent;
(2) Preparing fermented pine needle fine powder:
preparing raw materials in parts by mass, ventilating and drying pine needles and animal manure for 6-8 hours, and respectively putting into a pulverizer to pulverize into fine powder of 1-2 mm; spreading a layer of crushed animal manure fine powder on the bottom layer of the fermentation tank, placing 100Kg of crushed pine needle fine powder into the fermentation tank, adding crushed animal manure fine powder with the total volume of 40%, stirring and mixing uniformly, adding urea in the stirring process, adding potassium dihydrogen phosphate solution with the mass concentration of 0.2-0.5% to cause fermentation substrate to be moist, adding the microbial agent prepared in the step (1) to stir uniformly, ventilating and culturing for 3 days, adding potassium dihydrogen phosphate solution with the mass concentration of 0.2-0.5% to cause fermentation substrate to be moist again, compacting the fermentation substrate, sealing a plastic film, pressing soil and sealing, fermenting and culturing for 5-10 days, and naturally drying the fermentation product until the water content is 1-3%, thereby obtaining the fermented pine needle fine powder;
(3) Preparing fermented pine needle fine powder containing chelating water-retaining agent:
preparing raw materials in parts by mass, sequentially adding sulfur powder, oxalic acid, humic acid, chelating agent and water-retaining agent into the fermented pine needle fine powder prepared in the step (2), and stirring for 30-45min to obtain the fermented pine needle fine powder containing the chelating water-retaining agent;
(4) Granulating:
and (3) adding the fermented pine needle fine powder mixture containing the chelating water-retaining agent, which is prepared in the step (3), into a dry rolling granulator for granulation, and screening out particles with the particle size of 1-4.75mm to obtain the green efficient soil phosphate fertilizer synergist.
The phosphorus fertilizer has poor mobility in soil, is easy to fix and has low utilization rate. The fixation of phosphate fertilizer is affected by various factors, such as soil pH, clay content, organic matter content, free iron-aluminum oxide, calcium carbonate (CaCO) 3 ) The factors such as fertilization mode and the like can influence the rapid fixation of phosphorus in soil, wherein the phosphorus is divided into organic phosphorus and inorganic phosphorus, the inorganic phosphorus accounts for about 60-80% of the total phosphorus content, the inorganic phosphorus can be divided into soluble phosphorus and insoluble phosphorus, the insoluble phosphorus is divided into slow-release phosphorus and ineffective phosphorus, and at present, ca is widely considered as Ca 2 -P、Ca 8 -P、Al-P、Fe-P is a slow-release phosphorus source of plants; while Ca 10 P, O-P is difficult to use by plants and is considered to be phosphorus in an inactive state. The existence forms of phosphorus are also greatly different in different soils, and in acid soils, the existence forms of phosphorus are mainly ferric phosphate salts (Fe-P) and aluminum phosphate salts (Al-P); in alkaline soil, mainly calcium phosphate salts, e.g. Ca 2 -P、Ca 8 -P and Ca 10 P, the phosphate radical is adsorbed and fixed by calcium carbonate in the soil to form Ca-P, and Ca-P can be quickly converted into Ca 2 -P，Ca 2 P can be further converted into less soluble Ca 8 P, finally generating more insoluble Ca 10 P, difficult to use. According to the invention, pine needles and sulfur powder are added into soil to enable surrounding soil to be acidic, alkaline soil is improved, the concentration of insoluble calcium carbonate is reduced, the adsorption sites of phosphorus are reduced, the activity of phosphorus is increased, and the added chelating agent tetra sodium glutamate diacetate and phosphate anions competitively complex with metal ions such as calcium, iron and aluminum in the soil, so that the formation of insoluble phosphate is reduced; oxalic acid is activated to dissolve insoluble phosphate; the humic acid contains a large amount of carboxyl, hydroxyl, methoxy, phenolic hydroxyl and some nitrogen-containing cyclic compound functional groups, has stronger ion exchange and metal ion chelating capacity, can enhance the acid-base buffering capacity of soil, improve the physicochemical property of the soil, simultaneously improve the utilization rate of phosphate fertilizer, and enhance the cold resistance and stress resistance of plants; the potassium dihydrogen phosphate supplements potassium, makes iron element in a dissolved state, promotes plants to absorb iron element in soil, and also adds carboxymethyl starch as a water-retaining agent, so that the water-retaining agent plays a role in promoting phosphate fertilizer synergists, and the mass ratio of the carboxymethyl starch to the water-retaining agent is 1:1:1, the microbial agent consisting of trichoderma viride, aspergillus niger and bacillus megaterium generates a large amount of oxalic acid, citric acid, plant enzymes and other enzymes in the process of growing and fermenting, and promotes the dissolution of insoluble phosphorus.
Advantageous effects
The green efficient soil phosphate fertilizer synergist produced by the invention is added with pine needles, microbial agents, chelating agents, humic acid, urea, oxalic acid, monopotassium phosphate, sulfur powder and water-retaining agents in the production process. Compared with the prior art, the phosphorus fertilizer synergist prepared by taking pine needles as raw materials has the advantages that waste resources of raw materials are utilized; the added trichoderma viride, aspergillus niger and bacillus megaterium are synergistic, so that the dissolution of insoluble phosphorus is obviously promoted; the water-retaining agent is added in the preparation process to play a role in retaining water, and potassium dihydrogen phosphate and urea are added to introduce potassium and nitrogen elements; humic acid, sulfur powder and animal manure improve soil, chelating agent combines metal ions, oxalic acid dissolves insoluble calcium carbonate, the content of soluble phosphorus in the soil is greatly improved, the raw materials are environment-friendly, pollution is avoided, and the method is convenient to use and suitable for wide application.
Detailed Description
The technical scheme of the invention is further described below with reference to specific examples:
example 1
The green efficient soil phosphate fertilizer synergist comprises the following raw materials, by weight, 40 parts of a regulator, 20 parts of animal manure, 1 part of a chelating agent, 2 parts of a microbial agent, 30 parts of oxalic acid, 10 parts of humic acid, 2 parts of a water-retaining agent, 15 parts of urea and 5 parts of potassium dihydrogen phosphate.
The regulator is pine needle and sulfur powder with the mass ratio of 50:1.
The pine needle comprises pine needle, pine stump, and pine withered and fallen material.
The chelating agent is tetrasodium glutamate diacetate.
The microbial agent is trichoderma viride, aspergillus niger and bacillus megaterium, and the volume ratio is 1:1:1.
the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC3.15484, the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 1, the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 3, the trichoderma viride is purchased from Beijing, the Korean region of North Chen, the west road, the collection number of CGMCC3.15663, the bacillus megaterium is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 3, and the collection number of CGMCC1.16094.
The water-retaining agent is carboxymethyl starch.
The preparation method of the green efficient soil phosphate fertilizer synergist comprises the following steps:
(1) And (3) preparing a microbial agent:
culturing Trichoderma viride, aspergillus niger and Bacillus megaterium in seed culture medium at 25deg.C, 25deg.C and 30deg.C for 24 hr, inoculating LB culture medium at 8% inoculum size, shake culturing at 25deg.C, 25deg.C and 30deg.C to bacterial concentration OD600 about 3.0, and culturing at 1:1:1, uniformly mixing the components in a volume ratio, and freeze-drying the components to obtain freeze-dried powder to obtain a microbial agent;
(2) Preparing fermented pine needle fine powder:
preparing raw materials in parts by mass, ventilating and drying pine needles and animal manure for 6 hours, and respectively putting the raw materials into a pulverizer to pulverize into fine powder of 1 mm; spreading a layer of crushed animal manure fine powder on the bottom layer of the fermentation tank, placing 100Kg of crushed pine needle fine powder into the fermentation tank, adding crushed animal manure fine powder with the total volume of 40%, stirring and mixing uniformly, adding urea in the stirring process, adding a potassium dihydrogen phosphate solution with the mass concentration of 0.2% to cause fermentation substrate to be wet, adding the microbial agent prepared in the step (1) to stir uniformly, ventilating and culturing for 3 days, adding a potassium dihydrogen phosphate solution with the mass concentration of 0.2% to cause fermentation substrate to be wet again, compacting the fermentation substrate, sealing a plastic film, pressing soil and sealing, fermenting and culturing for 5 days, and naturally drying the fermentation product until the water content is 1%, thereby obtaining the fermented pine needle fine powder;
(3) Preparing fermented pine needle fine powder containing chelating water-retaining agent:
preparing raw materials in parts by mass, sequentially adding sulfur powder, oxalic acid, humic acid, chelating agent and water-retaining agent into the fermented pine needle fine powder prepared in the step (2), and stirring for 30min to obtain the fermented pine needle fine powder containing the chelating water-retaining agent;
(4) Granulating:
and (3) adding the fermented pine needle fine powder mixture containing the chelating water-retaining agent, which is prepared in the step (3), into a dry rolling granulator for granulation, and screening out particles with the particle size of 1-4.75mm to obtain the green efficient soil phosphate fertilizer synergist.
Example 2
The green efficient soil phosphate fertilizer synergist comprises the following raw materials, by weight, 50 parts of a regulator, 60 parts of animal manure, 3 parts of a chelating agent, 4 parts of a microbial agent, 40 parts of oxalic acid, 20 parts of humic acid, 3 parts of a water-retaining agent, 18 parts of urea and 6 parts of potassium dihydrogen phosphate.
The regulator is pine needle and sulfur powder with the mass ratio of 50:1.
The pine needle comprises pine needle, pine stump, and pine withered and fallen material.
The chelating agent is tetrasodium glutamate diacetate.
The microbial agent is trichoderma viride, aspergillus niger and bacillus megaterium, and the volume ratio is 1:1:1.
the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC3.15484, the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 1, the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 3, the trichoderma viride is purchased from Beijing, the Korean region of North Chen, the west road, the collection number of CGMCC3.15663, the bacillus megaterium is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 3, and the collection number of CGMCC1.16094.
The water-retaining agent is carboxymethyl starch.
The preparation method of the green efficient soil phosphate fertilizer synergist comprises the following steps:
(1) And (3) preparing a microbial agent:
culturing Trichoderma viride, aspergillus niger and Bacillus megaterium in seed culture medium at 25deg.C, 25deg.C and 30deg.C for 36 hr, inoculating LB culture medium at 8% inoculum size, shake culturing at 25deg.C, 25deg.C and 30deg.C to bacterial concentration OD600 about 3.0, and culturing at 1:1:1, uniformly mixing the components in a volume ratio, and freeze-drying the components to obtain freeze-dried powder to obtain a microbial agent;
(2) Preparing fermented pine needle fine powder:
preparing raw materials in parts by mass, ventilating and drying pine needles and animal manure for 7 hours, and respectively putting the raw materials into a pulverizer to pulverize into fine powder of 2 mm; spreading a layer of crushed animal manure fine powder on the bottom layer of the fermentation tank, placing 100Kg of crushed pine needle fine powder into the fermentation tank, adding crushed animal manure fine powder with the total volume of 40%, stirring and mixing uniformly, adding urea in the stirring process, adding a potassium dihydrogen phosphate solution with the mass concentration of 0.3% to cause fermentation substrate to be wet, adding the microbial agent prepared in the step (1) to stir uniformly, ventilating and culturing for 3 days, adding a potassium dihydrogen phosphate solution with the mass concentration of 0.3% to cause fermentation substrate to be wet again, compacting the fermentation substrate, sealing a plastic film, pressing soil and sealing, fermenting and culturing for 8 days, and naturally drying the fermentation product until the water content is 2%, thereby obtaining the fermented pine needle fine powder;
(3) Preparing fermented pine needle fine powder containing chelating water-retaining agent:
preparing raw materials in parts by mass, sequentially adding sulfur powder, oxalic acid, humic acid, chelating agent and water-retaining agent into the fermented pine needle fine powder prepared in the step (2), and stirring for 40min to obtain the fermented pine needle fine powder containing the chelating water-retaining agent;
(4) Granulating:
and (3) adding the fermented pine needle fine powder mixture containing the chelating water-retaining agent, which is prepared in the step (3), into a dry rolling granulator for granulation, and screening out particles with the particle size of 1-4.75mm to obtain the green efficient soil phosphate fertilizer synergist.
Example 3
The green efficient soil phosphate fertilizer synergist comprises the following raw materials, by weight, 65 parts of a regulator, 100 parts of animal manure, 5 parts of a chelating agent, 6 parts of a microbial agent, 50 parts of oxalic acid, 30 parts of humic acid, 5 parts of a water-retaining agent, 20 parts of urea and 8 parts of potassium dihydrogen phosphate.
The regulator is pine needle and sulfur powder with the mass ratio of 50:1.
The pine needle comprises pine needle, pine stump, and pine withered and fallen material.
The chelating agent is tetrasodium glutamate diacetate.
The microbial agent is trichoderma viride, aspergillus niger and bacillus megaterium, and the volume ratio is 1:1:1.
the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC3.15484, the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 1, the trichoderma viride is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 3, the trichoderma viride is purchased from Beijing, the Korean region of North Chen, the west road, the collection number of CGMCC3.15663, the bacillus megaterium is purchased from China General Microbiological Collection Center (CGMCC) with the address of CGMCC No. 3, and the collection number of CGMCC1.16094.
The water-retaining agent is carboxymethyl starch.
The preparation method of the green efficient soil phosphate fertilizer synergist comprises the following steps:
(1) And (3) preparing a microbial agent:
culturing Trichoderma viride, aspergillus niger and Bacillus megaterium in seed culture medium at 25deg.C, 25deg.C and 30deg.C for 48 hr, inoculating LB culture medium at 8% inoculum size, shake culturing at 25deg.C, 25deg.C and 30deg.C to bacterial concentration OD600 about 3.0, and culturing at 1:1:1, uniformly mixing the components in a volume ratio, and freeze-drying the components to obtain freeze-dried powder to obtain a microbial agent;
(2) Preparing fermented pine needle fine powder:
preparing raw materials in parts by mass, ventilating and drying pine needles and animal manure for 8 hours, and respectively putting the raw materials into a pulverizer to pulverize into fine powder of 2 mm; spreading a layer of crushed animal manure fine powder on the bottom layer of the fermentation tank, placing 100Kg of crushed pine needle fine powder into the fermentation tank, adding crushed animal manure fine powder with the total volume of 40%, stirring and mixing uniformly, adding urea in the stirring process, adding a potassium dihydrogen phosphate solution with the mass concentration of 0.5% to cause fermentation substrate to be wet, adding the microbial agent prepared in the step (1) to stir uniformly, ventilating and culturing for 3 days, adding a potassium dihydrogen phosphate solution with the mass concentration of 0.5% to cause fermentation substrate to be wet again, compacting the fermentation substrate, sealing a plastic film, pressing soil and sealing, fermenting and culturing for 10 days, and naturally drying the fermentation product until the water content is 3%, thereby obtaining the fermented pine needle fine powder;
(3) Preparing fermented pine needle fine powder containing chelating water-retaining agent:
preparing raw materials in parts by mass, sequentially adding sulfur powder, oxalic acid, humic acid, chelating agent and water-retaining agent into the fermented pine needle fine powder prepared in the step (2), and stirring for 45min to obtain the fermented pine needle fine powder containing the chelating water-retaining agent;
(4) Granulating:
and (3) adding the fermented pine needle fine powder mixture containing the chelating water-retaining agent, which is prepared in the step (3), into a dry rolling granulator for granulation, and screening out particles with the particle size of 1-4.75mm to obtain the green efficient soil phosphate fertilizer synergist.
Comparative examples 1 to 12
The composition and the proportion of the microbial agent are changed to prepare the antibacterial sustained and controlled release fertilizer, and other raw materials and preparation methods are the same as those of the example 3. Namely, in the preparation method of the microbial agent: culturing Trichoderma viride, aspergillus niger and Bacillus megaterium in seed culture medium at 25deg.C, 25deg.C and 30deg.C for 48 hr, inoculating LB culture medium according to 8% inoculum size, shake culturing at 25deg.C, 25deg.C and 30deg.C until the bacterial concentration OD600 is about 3.0, and mixing according to different volume ratios to obtain corresponding proportion.
The microbial agent composition is shown in table 1:
table 1 microbial inoculant composition (volume ratio)
| Trichoderma viride | Aspergillus niger | Bacillus megaterium | |
| Comparative example 1 | 1 | 0 | 0 |
| Comparative example 2 | 0 | 1 | 0 |
| Comparative example 3 | 0 | 0 | 1 |
| Comparative example 4 | 1 | 1 | 0 |
| Comparative example 5 | 0 | 1 | 1 |
| Comparative example 6 | 1 | 0 | 1 |
| Comparative example 7 | 1 | 2 | 1 |
| Comparative example 8 | 2 | 1 | 1 |
| Comparative example 9 | 1 | 1 | 2 |
| Comparative example 10 | 2 | 2 | 1 |
| Comparative example 11 | 2 | 1 | 2 |
| Comparative example 12 | 1 | 2 | 2 |
Detection experiment:
2.4g of monoammonium phosphate (N-P-K: 12-61-0, total nutrient 73%) and 8000g of dry acid soil (pH < 5) are stirred and mixed uniformly, 16 parts of 500g of phosphorus-containing fertilizer soil are evenly separated and placed in 16 identical containers with small round holes at the bottom, the water content of the soil is adjusted to 18% -22% by a weight method, the phosphorus fertilizer synergistic agent prepared in the example 1-3 and the comparative example 1-12 are respectively added into each container, and then all the soil added with the phosphorus fertilizer synergistic agent and blank control soil without any phosphorus fertilizer synergistic agent are inspected and measured once every other week according to the method of GB 12297-1990.
2.4g of monoammonium phosphate (N-P-K: 12-61-0, total nutrient 73%) and 8000g of dry alkaline soil (pH > 7) are stirred and mixed uniformly, 16 parts of 500g of phosphorus-containing fertilizer soil are evenly separated and placed in 16 identical containers with small round holes at the bottom, the water content of the soil is adjusted to 18% -22% by a weight method, the phosphorus fertilizer synergistic agent prepared in the examples 1-3 and the comparative examples 1-12 are respectively added into each container, and then all the soil added with the phosphorus fertilizer synergistic agent and blank control soil without any phosphorus fertilizer synergistic agent are inspected and measured once every other week according to the method of GB 12297-1990.
The test results of the available phosphorus content in the dry acid soil and the alkaline soil are shown in table 2:
TABLE 2 Table for measuring content of available phosphorus in acidic and alkaline soil
The content of available phosphorus in the soil of each container was measured by inspection at weekly intervals according to the method of GB 12297-1990. The detection result shows that the volume ratio of the trichoderma viride, the aspergillus niger and the bacillus megaterium in the added phosphate fertilizer synergist in the embodiment 3 is 1:1:1, the content of the effective phosphorus in the acid and alkali soil is obviously higher than that of the phosphate fertilizer synergist of the control group 1-12 and the blank group. Compared with the method without adding phosphate fertilizer synergist, the effective phosphorus content in the acid soil is improved by 105 percent, and the effective phosphorus content in the alkaline soil is improved by 57.3 percent, so that the volume ratio of the trichoderma viride, aspergillus niger and bacillus megaterium in the phosphate fertilizer synergist microbial inoculum is 1:1:1, the prepared green high-efficiency soil phosphate fertilizer synergist can effectively activate phosphorus fixed in soil by phosphate fertilizer, reduce the content of the fixed phosphorus in the soil and has better fertilization effect.
Field experiment:
the phosphate fertilizer potentiators prepared in examples 1 to 3 and comparative examples 1 to 12 of the present invention were used in home farms in the village, where Dou Zhen c.f. was located in mongolian county. Corn is planted in 32 mu of land with the same planting environment, corn seeds are purchased from a smooth jade 698 produced by Henan smooth and rich seed industry limited company, 2021, 5 months and 5 days are sowed, row spacing is 60cm, plant spacing is 36cm,5 months and 21 days are sowed, 32 mu of land is divided into 16 areas with the size of 2 mu, phosphate fertilizer synergists prepared in examples 1-3 and comparative examples 1-12 and a blank group without adding any phosphate fertilizer synergist are respectively applied to the 16 lands together with fertilizers, 10Kg of monoammonium phosphate (Shandong Wan biosciences limited company, N-P-K:12-61-0, total 73%) and 100g of phosphate fertilizer synergist are uniformly mixed and applied, 10Kg of urea and 15Kg of potassium fertilizer are applied to each mu of land, the whole land is subjected to one-time fertilization, and the corn growth period management is carried out according to a conventional cultivation technology, and the lodging numbers of corn stalks are respectively carried out on each land; detecting one plant at intervals of 5m, and detecting the average value of leaf width and chlorophyll content of 15 plants in the three-leaf period and the six-leaf period; one strain is detected at intervals of 5m, the weight average value detection statistics of each ear of grains of 15 strains are detected, and the detection results are shown in table 3:
TABLE 3 statistical table of maize growth conditions and yield
As can be seen from experimental detection results, the green high-efficiency soil phosphorus fertilizer synergist prepared in the application examples 1-3 is compared with the phosphorus fertilizer synergist prepared in the comparison examples 1-12 and the blank group, and the corn planted by the green high-efficiency soil phosphorus fertilizer synergist prepared in the application examples 1-3 has fewer lodging numbers, leaf widths, chlorophyll content and higher weight per spike seed. Therefore, the phosphate fertilizer synergist produced by the invention can effectively activate the fixed phosphorus in soil and phosphate fertilizer, promote plant absorption, make plant growth more healthy and strong, not easy to lodge, make seeds more plump, and have higher yield, the average per mu yield of corn can reach 1100Kg, the yield of corn without phosphate fertilizer synergist is improved by 37%, and the raw materials used by the invention are environment-friendly, free of pollution, convenient to use and suitable for wide application.
It should be noted that the above-mentioned embodiments are merely some, but not all embodiments of the preferred mode of carrying out the invention. It is evident that all other embodiments obtained by a person skilled in the art without making any inventive effort, based on the above-described embodiments of the invention, shall fall within the scope of protection of the invention.
Claims (5)
1. The green efficient soil phosphate fertilizer synergist is characterized by comprising the following raw materials, by weight, 40-65 parts of a regulator, 20-100 parts of animal manure, 1-5 parts of a chelating agent, 2-6 parts of a microbial agent, 30-50 parts of oxalic acid, 10-30 parts of humic acid, 2-5 parts of a water-retaining agent, 15-20 parts of urea and 5-8 parts of monopotassium phosphate;
the microbial agent is trichoderma viride, aspergillus niger and bacillus megaterium, and the volume ratio is 1:1:1, a step of;
the preservation number of the trichoderma viride is CGMCC3.15484, the preservation number of the aspergillus niger is CGMCC3.15663, and the preservation number of the bacillus megaterium is CGMCC1.16094.
2. The green efficient soil phosphorus fertilizer synergist according to claim 1, wherein the regulator is pine needle and sulfur powder in a mass ratio of 50:1.
3. The green high-efficiency soil phosphate fertilizer synergist according to claim 1, wherein the chelating agent is tetrasodium glutamate diacetate.
4. The green efficient soil phosphate fertilizer synergist according to claim 1, wherein the water-retaining agent is carboxymethyl starch.
5. A method for preparing the green high-efficiency soil phosphate fertilizer synergist according to any one of claims 1-4, comprising the following steps:
(1) And (3) preparing a microbial agent:
culturing Trichoderma viride, aspergillus niger and Bacillus megaterium in seed culture medium at 25deg.C, 25deg.C and 30deg.C for 24-48 hr, inoculating LB culture medium at 8% inoculum size, shake culturing at 25deg.C, 25deg.C and 30deg.C until the bacterial concentration OD600 is about 3.0, and mixing with the culture medium according to the ratio of 1:1:1, uniformly mixing the components in a volume ratio, and freeze-drying the components to obtain freeze-dried powder to obtain a microbial agent;
(2) Preparing fermented pine needle fine powder:
preparing raw materials in parts by mass, ventilating and drying pine needles and animal feces in a regulator for 6-8 hours, and respectively putting the raw materials into a pulverizer to pulverize into fine powder of 1-2 mm; spreading a layer of crushed animal manure fine powder on the bottom layer of the fermentation tank, placing 100Kg of crushed pine needle fine powder into the fermentation tank, adding crushed animal manure fine powder with the total volume of 40%, stirring and mixing uniformly, adding urea in the stirring process, adding potassium dihydrogen phosphate solution with the mass concentration of 0.2-0.5% to cause fermentation substrate to be moist, adding the microbial agent prepared in the step (1) to stir uniformly, ventilating and culturing for 3 days, adding potassium dihydrogen phosphate solution with the mass concentration of 0.2-0.5% to cause fermentation substrate to be moist again, compacting the fermentation substrate, sealing a plastic film, pressing soil and sealing, fermenting and culturing for 5-10 days, and naturally drying the fermentation product until the water content is 1-3%, thereby obtaining the fermented pine needle fine powder;
(3) Preparing fermented pine needle fine powder containing chelating water-retaining agent:
preparing raw materials in parts by mass, sequentially adding sulfur powder, oxalic acid, humic acid, chelating agent and water-retaining agent in the regulator into the fermented pine needle fine powder prepared in the step (2), and stirring for 30-45min to obtain the fermented pine needle fine powder containing the chelating water-retaining agent;
(4) Granulating:
and (3) adding the fermented pine needle fine powder mixture containing the chelating water-retaining agent, which is prepared in the step (3), into a dry rolling granulator for granulation, and screening out particles with the particle size of 1-4.75mm to obtain the green efficient soil phosphate fertilizer synergist.
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| AU2007209830A1 (en) * | 2006-08-17 | 2008-03-06 | The Era Farming Company Pty Ltd | Fertiliser composition |
| CN105330413A (en) * | 2015-11-05 | 2016-02-17 | 宁国市汉唐盛世农林开发有限公司 | Phosphate-solubilizing nutrient soil and preparation method thereof |
| CN105441330A (en) * | 2015-11-19 | 2016-03-30 | 中国农业科学院农业资源与农业区划研究所 | Efficient phosphorus-dissolving growth-promoting bacterium and bio-fertilizer prepared from same as well as application of efficient phosphorus-dissolving growth-promoting bacterium |
| CN113774050A (en) * | 2021-09-11 | 2021-12-10 | 成都云图控股股份有限公司 | Microbial agent for promoting nutrient absorption |
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