CN116672760B - Solid phase extraction instrument - Google Patents

Abstract

The utility model provides a solid-phase extraction appearance, belongs to chromatographic separation technical field, and it includes extraction portion, is provided with three solenoid valve in the extraction portion, installs the gate type frame on the extraction portion, and horizontal lead screw is installed to the upper end of gate type frame, and vertical lead screw is installed to the output of horizontal lead screw, is equipped with pipetting mechanism on the vertical lead screw, still installs a plurality of placers on the extraction portion, has placed extraction portion on the placer, and one side of extraction portion is equipped with placement portion, and the other side of extraction portion is equipped with out liquid portion. Distilled water in the water tank conveniently passes through the liquid inlet pipe, the inside of the extraction mechanism and the liquid discharge pipe through the three-way electromagnetic valve, the parts passing through the liquid inlet pipe, the inside of the extraction mechanism and the liquid discharge pipe are cleaned, impurities can be prevented from entering the target extraction solution again in the eluting process through cleaning, and therefore the purity of the target extract is improved. And the distilled water can be prevented from entering the extraction part in the cleaning process through the arrangement of the three-way electromagnetic valve, so that the extraction is influenced.

Description

Solid phase extraction instrument

Technical Field

The invention relates to the technical field of chromatographic separation, in particular to a solid phase extraction instrument.

Background

The solid phase extraction is a chromatographic separation technology for enriching, separating and purifying a sample by the characteristics of selective adsorption and selective elution of an extraction column.

At present, chromatographic separation operations are often carried out by means of solid phase extractors in solid phase extraction. In operation, the extraction column is first wetted with a wetting and activating agent to remove impurities from the extraction column. And then the extraction solution passes through the extraction column, so that the extraction column adsorbs the target object. And leaching the extraction column in a leaching mode to leach non-target substances on the extraction column. And finally, repeatedly eluting the extraction column in a repeated eluting mode, and obtaining a target object from the eluted solution.

At present, when the operation is performed, the internal pipeline of the solid phase extraction instrument and the like are not cleaned, so that the content of impurities in the target object is high, and part of the impurities in the activator carrying extraction column are adhered to the inner wall of the pipeline and the like, so that part of the impurities enter an eluting solution during the subsequent elution. Or part of the leached impurities in the leaching solution adhere to the pipeline, so that the impurities finally enter the eluting solution in the eluting process, and the purity of the target object is finally influenced.

Meanwhile, when the currently adopted solid phase extraction instrument is used for activation, leaching and elution, a manual pipetting mode is adopted, and the mode is low in efficiency at first.

Again, most of the extraction column placement members currently employed are open at the upper end, and of course, such designs facilitate the injection of eluents and the like by the operator, but during operation, some of the impurities in the air are liable to enter the placement members, thereby further reducing the purity of the obtained target.

Finally, most of the extraction columns adopted at present are directly spliced on the rubber joint, and in the pipetting process, the placing component is easy to incline, so that the solution in the extraction column is poured out, and the extraction amount of the target object is reduced.

Disclosure of Invention

The invention provides a solid phase extraction instrument, which solves the defects of the prior art, and the cleaning steps are introduced between each two steps so as to clean objects such as pipelines, thereby improving the extraction purity of the target objects. Thereby ensuring safety in operation.

In order to achieve the object of the present invention, the following techniques are proposed:

a solid phase extraction apparatus comprising: the extraction portion, including the bottom plate, the left and right sides of bottom plate is equipped with the end plate respectively, and the upper end of end plate is equipped with the apron, and both sides are equipped with the curb plate respectively around the bottom plate, installs a plurality of extraction mechanism on the bottom plate, and extraction mechanism's lower extreme is connected with the fluid-discharge head, is connected with the fluid-discharge tube on the fluid-discharge head, and the outside end of fluid-discharge tube wears to be on a curb plate, install the fixed plate on the curb plate, installs the water tank on the apron, and the water tank intussuseption is filled with distilled water, and extraction mechanism's one end is connected with the feed liquor head, and the upper end intercommunication of feed liquor head has the feed liquor pipe, and the upper end of feed liquor pipe is connected with three-way solenoid valve, and three-way solenoid valve's one end intercommunication has first branch pipe, and first branch pipe wears to be in the apron, and three-way solenoid valve's the other end intercommunication has the second branch pipe, and the other end of second branch pipe wears to be in the apron and communicate in the water tank. The setting of three-way solenoid valve conveniently carries out the intercommunication or close of first branch pipe and second branch pipe and feed liquor pipe, conveniently makes the distilled water in the water tank pass through feed liquor pipe, extraction mechanism inside and fluid-discharge tube through three-way solenoid valve to wash above-mentioned part that passes through, can avoid impurity reentrant target extraction solution at the in-process of elution through wasing, thereby promote the purity of target extract. And the distilled water can be prevented from entering the extraction part in the cleaning process through the arrangement of the three-way electromagnetic valve, so that the extraction is influenced.

The upper end of the door-shaped frame is provided with a transverse screw, the output end of the transverse screw is provided with a vertical screw, the vertical screw is provided with a pipetting mechanism, and the cover plate is provided with a plurality of placing mechanisms. The extraction parts are placed on the placement mechanism, the lower ends of the extraction parts are connected to the first branch pipes, the number of the extraction parts is consistent with that of the first branch pipes, the first branch pipes are in one-to-one correspondence with the extraction parts, and the extraction parts are communicated with the first branch pipes corresponding to the extraction parts. One of the side plates is provided with a placing part, and the placing part is provided with at least four placing bottles. The other side plate is provided with a liquid outlet part which is communicated with the liquid discharge pipe. The transverse screw rod can drive the liquid moving mechanism to transfer the activating agent, the sample, the leaching agent and the eluting agent into each extraction part from the placement part, so that the extraction column or the filling material in the extraction part is activated, or the sample is injected, or the eluting agent is used for eluting impurities, or the eluting agent is used for eluting the target object, and the efficiency of extracting the target object is improved in the mode. The vertical screw rod can drive the liquid transfer mechanism to move downwards, so that liquid is sucked and extruded through the liquid transfer mechanism. The extraction part is injected by extrusion, and the lower end of the extraction part adopts a sealing mode, so that dust and the like in the air can be prevented from entering relevant liquid in the extraction part. The placing mechanism can be used for placing the extraction part stably, so that the extraction part is prevented from tilting in the process of pipetting injection. The placement portion provides placement locations for placement of the activator, sample, eluent, and eluent, while also facilitating the aspiration of the activator, sample, eluent, and eluent therein by the pipetting mechanism. The liquid outlet part is convenient for discharging liquid when discharging different solutions, and the operation such as cleaning the inside of the liquid outlet part through distilled water is convenient.

The transverse screw rod is used for adjusting the position of the liquid-moving mechanism, so that the liquid-moving mechanism transfers the liquid in the placing bottle into the extraction part, the first branch pipe is communicated with the liquid inlet pipe through the three-way electromagnetic valve, the liquid is conveyed into the liquid outlet part through the liquid outlet pipe through the extraction mechanism and is discharged from the liquid outlet part, after the liquid in the extraction part is discharged, the communication between the first branch pipe and the liquid inlet pipe is closed through the three-way electromagnetic valve, and the communication between the second branch pipe and the liquid inlet pipe is opened, so that distilled water washes the inside of the extraction mechanism and the liquid outlet pipe.

Further, the extraction mechanism comprises an installation table installed on the bottom plate, a pair of installation seats are installed at one end of the installation table, bearing seats are installed at the upper ends of the installation seats respectively, rotating shafts are arranged on the bearing seats in a rotating mode, the rotating shafts are disconnected, and action interference caused by rotation of the rotating arms is avoided. One end of the rotating shaft is connected with a motor, the motor is installed on the bottom plate through the mounting plate, the rotating shaft is connected with a rotating arm, the other end of the rotating arm is provided with a connecting shaft, the connecting shaft is rotatably provided with a connecting arm, the other end of the connecting arm is rotatably provided with a connecting seat, and a movable rod is installed on the connecting seat. The rotating arm, the connecting arm and the connecting seat can enable the movable rod to reciprocate under the rotation of the rotating arm, so that liquid extraction operation is facilitated.

The piston is installed to the other end of movable rod, the periphery of piston is equipped with a plurality of first sealing rings, the cover is equipped with the end cover on the movable rod, be equipped with the sealing ring in the end cover, the sealing ring cover is located on the movable rod, the intercommunication has first round hole on the piston, the inboard end intercommunication of first round hole has the second round hole, the intercommunication has the third round hole on the second round hole, second round hole and third round hole take shape on the movable rod, the aperture of second round hole is greater than the aperture of first round hole, be equipped with first sealing ball in the second round hole, be equipped with first spring in the second round hole, first sealing ball is located the outside end of first spring, and first sealing ball is used for the shutoff or the intercommunication of first round hole and second round hole, install movable housing on the end cover, be equipped with movable round hole in the movable housing, the piston is movable round hole internal motion, the flowing back head spiro union is in movable housing's lower extreme, and flowing back head and movable round hole intercommunication.

The second end cover is installed to the other end of movable housing, second end cover upper end opening takes shape and has the fourth round hole, feed liquor head spiro union in the upper end of fourth round hole, the lower extreme intercommunication of fourth round hole has the fifth round hole, the aperture of fifth round hole is greater than the aperture of fourth round hole, the lower extreme of fifth round hole is equipped with sealed end, sealed end spiro union is in the lower extreme of second end cover, be equipped with the second spring in the fifth round hole, the upper end of second spring is equipped with the second sealing ball, the second sealing ball is located the fifth round hole, and the second sealing ball is used for the intercommunication or the closure of fifth round hole and fourth round hole, the lower extreme intercommunication of fifth round hole has the sixth round hole, sixth round hole and movable round hole intercommunication. When the piston moves to the second end cover, the piston moves backwards due to the increase of pressure, so that liquid enters the movable round hole through the first round hole, the second round hole and the third round hole, and when the piston is far away from the second end cover, the first sealing ball seals the first round hole and the second round hole, and liquid is discharged from the liquid discharge head in an extrusion mode. When liquid is fed, the piston moves away from the second end cover, the second sealing ball moves downwards through the movement, so that the fifth round hole is communicated with the fourth round hole, liquid enters the movable round hole through the sixth round hole, and the liquid is ready to be discharged.

Further, the upper top one end of the water tank is provided with a liquid injection head, the upper end of the liquid injection head is provided with a liquid injection cover, impurities in the air and the like are prevented from entering the water tank by the arrangement of the liquid injection cover, and therefore the cleaning effect is affected. The top other end of the water tank is provided with an air inlet column, filter cotton is filled in the air inlet column, impurities in the air can be adsorbed by the aid of the filter cotton, pollution to the inside of the water tank is avoided, and meanwhile distilled water is conveniently sucked through the arrangement of the air inlet column.

Further, a mounting backboard is arranged at the output end of the vertical screw rod, a pair of fixed arc plates are arranged on the mounting backboard, and the fixed arc plates are fixed through screws.

The pipetting mechanism comprises a pipette fixed through a fixed arc plate, and the pipette is conveniently fixed through the fixed arc plate. The lower extreme of pipette is equipped with the liquid moving head, the upper end spiro union of pipette has the lid, the lid is connected with the upper plate through many middle levers, install the liquid moving motor on the upper plate, the output shaft of liquid moving motor has drive gear, drive gear meshing has driven gear, be equipped with the silk shell on the driven gear, the silk shell rotates to locate on the upper plate, the spiro union has down the lead screw in the silk shell, the lead screw wears on the lid down, the lower extreme of lead screw is equipped with the extrusion dish down, the extrusion dish is located the pipette, the upper end of lead screw is equipped with the fly leaf down, be equipped with a pair of guide bar on the fly leaf, the guide bar wears on the upper plate, the guide bar plays the effect of motion direction to the motion of extrusion dish. Through pushing down lead screw and silk cover transmission's mode, conveniently carry out the accurate injection of liquid, also improved stability simultaneously.

Further, the extraction part comprises an extraction pipe, an extraction column is filled at the lower end of the inside of the extraction pipe, a cannula is arranged at the lower end of the extraction pipe, and a sealing piece is arranged at the upper end of the extraction pipe. The sealing piece is used for sealing the upper end of the extraction tube, so that air impurities can be prevented from entering the tube, and the purity of an extracted target object is improved.

Further, the pipette head comprises a mounting ring arranged at the lower end of the pipette, a first eyelet is arranged on the mounting ring, a lower extension pipe is arranged at the lower end of the mounting ring, a second eyelet, a third eyelet and a fourth eyelet are sequentially arranged in the lower extension pipe from top to bottom, the second eyelet is communicated with the first eyelet, the third eyelet is communicated with the second eyelet, the fourth eyelet is communicated with the third eyelet, the aperture of the second eyelet is larger than that of the first eyelet, the aperture of the second eyelet is larger than that of the third eyelet, the aperture of the third eyelet is larger than that of the fourth eyelet, a third spring is arranged in the second eyelet, a third sealing ball is arranged at the lower end of the third spring and used for controlling the sealing or the communication of the second eyelet and the third eyelet, a movable pipe is arranged in the fourth eyelet, a convex ring is arranged at the upper end of the movable pipe and is positioned in the third eyelet, a liquid inlet notch is formed in the upper end of the movable pipe, a middle part of the periphery of the movable pipe is provided with a middle ring, and the lower end of the movable pipe is provided with a liquid outlet notch. Under normal conditions, such as when transferring liquid, the third sealing ball seals the second eyelet and the third eyelet under the action of the third spring, avoiding impurities entering the pipette. When the lower end of the movable pipe is provided with the supporting part, the third sealing ball can be pushed to move upwards when the middle ring is supported by the supporting part, so that the blocking is canceled, and the liquid can be conveniently discharged and enter.

Further, the sealing piece comprises a screw joint ring which is in screw joint with the upper end of the inside of the extraction pipe, a plurality of air holes are formed in the screw joint ring, an inner pipe is arranged in the screw joint ring, a sealing cover is in screw joint with the upper end of the inner pipe, a fifth eyelet is arranged at the lower end of the inner pipe, the upper end of the fifth eyelet is communicated with a sixth eyelet, a cavity is formed in the sealing cover, the sixth eyelet is communicated with the cavity, a seventh eyelet is formed at the upper end of the sealing cover, a fourth spring is arranged in the sixth eyelet, a fourth sealing ball is arranged at the upper end of the fourth spring, and the fourth sealing ball is used for controlling the communication or sealing between the seventh eyelet and the cavity. The liquid outlet notch and the liquid inlet notch arranged on the movable tube are arranged to prevent the third sealing ball or the fourth sealing ball from blocking the two ends of the movable tube, so that the extrusion operation of liquid is convenient. When under normal conditions, such as liquid extraction, the fourth sealing ball can seal the seventh hole and the cavity due to the existence of the air holes, so that impurities are prevented from entering in the sealing way, and the air holes have smaller hole diameters, and if substances such as corresponding filter cotton can be arranged on the air holes for improving the effect. The air holes are arranged, so that air can be conveniently discharged when liquid is injected. And when liquid is injected, the movable pipe can push the fourth sealing ball to move downwards, so that the blocking of the seventh hole and the cavity is eliminated, the injection of liquid is convenient, and the liquid is mainly discharged from the liquid outlet notch of the movable pipe during the injection.

The fifth eyelet is the toper structure, and the upper end is the big end, and this kind of structural design can show the shutoff effect that improves fourth sealing ball, certainly in order to improve sealing effect foretell first sealing ball, second sealing ball, third sealing ball's sealing contact hole site department all can set up to the toper structure.

When the liquid of the pipette is squeezed in, the lower end of the movable tube is positioned in the cavity, the upper end of the movable tube is positioned in the second eyelet, the liquid enters the movable tube from the liquid inlet notch, and the liquid is discharged from the liquid outlet notch to the sixth eyelet.

Further, the placing mechanism comprises a mounting frame arranged on the cover plate, a plurality of rubber heads are arranged on the mounting frame, and the insertion pipe penetrates through the rubber heads. The rubber head is connected in first branch pipe upper end, install many connecting rods on the mounting bracket, go up the cross-plate on the upper end of connecting rod is installed, go up to wear to have a pair of round pin of wearing on the cross-plate, the lower extreme of wearing the round pin is equipped with first cylinder, the lower extreme of first cylinder is equipped with the toper post, the lower extreme of toper post is the tip, the lower extreme of toper post is equipped with the connecting rod, the lower extreme of connecting rod is equipped with the arch, be equipped with down the cross-plate between the arch, a pair of spill frame is installed to the lower wall of going up the cross-plate, the vertical section lower extreme of spill frame is equipped with interior the stretching plate, interior stretching plate's inboard end rotation is equipped with the rotor plate, the upper end of rotor plate is equipped with the gyro wheel, the periphery and the connecting rod of gyro wheel, toper post and first cylinder are tangent, the lower extreme of rotor plate is buckled and is installed the location arc board between every installation curb plate, the rotor plate inner wall is equipped with the connecting pin, be equipped with the extension spring between every pair of connecting pins that are located homonymy. The lower plate mentioned therein acts as a rigid stop for the cannula. The upper penetrating plate plays a limiting and restraining role on the upper end of the extraction tube. When the extraction tube is inserted and placed, the reinforced limit can be performed through the components, and the first cylinder can act on the roller due to the movement of the connecting rod, the conical column and the first cylinder, so that the positioning arc plate is used for further limiting the extraction tube, and the limiting constraint effect is remarkably improved through the operation mode.

When the extraction part is placed on the placement mechanism, the extraction pipe penetrates through the upper penetrating plate, and the insertion pipe penetrates through the lower penetrating plate. When the roller acts on the first cylinder, the positioning arc plate acts on the periphery of the extraction tube for limiting the extraction tube. When the roller acts on the connecting rod, the positioning arc plate is in an open state.

Further, place the portion including installing the installation hypoplastron on the curb plate, the outside end of installation hypoplastron is equipped with the restriction ring, places the bottle and places in the restriction ring, and the restriction ring can restrict placing the bottle to avoid taking place to empty risks such as dropping.

The upper end of the placing bottle is provided with a rubber plug, the rubber plug is provided with a liquid taking pipe and a ventilation head, and the ventilation head is convenient for air to enter, so that the liquid outlet rate is improved.

An upper extension table is arranged at one end of the mounting frame, at least four hoses are penetrated on the upper extension table, the upper end of each hose is provided with an extraction head, and the other ends of the hoses are respectively communicated with the liquid taking pipe. The lower end of the movable tube is inserted on the extraction head to facilitate the liquid extraction operation.

Further, the liquid outlet part comprises a lifting screw rod arranged on the side plate, the output end of the lifting screw rod is provided with a movable concave part, two ends of the movable concave part are respectively provided with a rotating shaft seat, one rotating shaft seat is provided with a rotating end seat, the rotating end seat is provided with a liquid outlet motor, the output shaft of the liquid outlet motor is provided with a rectangular column, and two ends of the rectangular column are rotationally arranged on the rotating shaft seat. Four side walls of the rectangular column are provided with a plurality of plugs, the rectangular column is provided with four long holes, the long holes are respectively communicated with the plugs adjacent to the long holes, one end of each long hole is open, a connecting plug is arranged at the open end, the outer side end of the liquid discharge pipe penetrates through the plug, and liquid is discharged through the connecting plug. The height of the rectangular column can be adjusted through the arrangement of the lifting screw rod, so that the spigot is conveniently inserted into the liquid discharge pipe, and the liquid discharge operation is convenient to conduct. Meanwhile, by adopting a separation and discharge mode, the collection of liquid in and out is convenient, and the occurrence of cross contamination and other conditions is avoided.

The technical scheme has the advantages that:

when the chromatographic separation technology is used for extracting the target, the automatic addition of the activating agent, the eluting agent and the cleaning water is convenient and automatic, so that the efficiency of extraction is improved. And the upper end of the placement part is sealed, so that the influence of impurities and the like on the extraction purity of the target object is avoided. Meanwhile, the activator, the eluent and the eluent are washed by washing water after passing through, so that the extraction purity of the target object is improved. And the extraction part is supported in a reinforcing way, so that the extraction part is ensured not to incline in the pipetting process.

Drawings

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings.

Fig. 1 shows one of the perspective views of a solid phase extractor in a three-dimensional structure.

Fig. 2 shows another perspective view of the solid phase extraction apparatus from a perspective view.

Fig. 3 is a perspective view showing the inside of the extracting portion.

Fig. 4 shows a perspective view of the drawing mechanism.

Fig. 5 shows a longitudinal section of the extraction mechanism.

Fig. 6 shows a partial longitudinal section in the extraction mechanism.

Fig. 7 shows a perspective view of the structure of the components such as the lateral screw and the vertical screw.

Fig. 8 shows one of the perspective views of the perspective structure of the pipetting mechanism.

Fig. 9 shows another perspective view of a pipetting mechanism.

Fig. 10 shows a cross-sectional view of the pipetting head.

FIG. 11 shows a cross-sectional view of a pipetting head and an extraction unit where 11-a shows a cross-sectional view of the pipetting head; 11-b shows a cross-sectional view of the extraction section.

Fig. 12 shows a perspective view of the placement mechanism.

Fig. 13 shows one of the perspective views of the perspective view of the single placement mechanism.

Fig. 14 shows another perspective view of the single placement mechanism.

Fig. 15 shows a perspective view of the placement unit.

Fig. 16 shows a perspective view of the liquid outlet portion.

Fig. 17 is a partially developed view of the liquid outlet portion.

Reference numerals illustrate:

the drawing part 1, the bottom plate 100, the end plate 101, the side plate 102, the cover plate 103, the mount 104, the mount 106, the motor 107, the rotation shaft 108, the mount 109, the bearing housing 110, the rotation arm 111, the connection shaft 112, the connection arm 113, the connection housing 114, the movable rod 115, the end cap 116, the seal ring 117, the piston 118, the first round hole 119, the second round hole 120, the third round hole 121, the first seal ball 122, the first seal ring 123, the movable housing 124, the movable round hole 125, the liquid discharge head 126, the liquid discharge tube 127, the second end cap 128, the fourth round hole 129, the liquid inlet head 130, the fifth round hole 131, the second spring 132, the second seal ball 133, the sixth round hole 134, the liquid inlet tube 135, the three-way solenoid valve 136, the first branch 137, the second branch 138, the water tank 139, the air inlet column 140, the liquid injection head 141, the fixed plate 142, the first spring 143, the gate frame 2, the lateral screw 3, the vertical screw 4, the mount back plate 40, fixed arc plate 41, screw 42, pipetting mechanism 5, pipette 500, cap 501, center bar 502, upper plate 503, pipetting motor 504, drive gear 505, driven gear 506, wire sleeve 507, down-feed screw 508, movable plate 509, guide bar 510, mounting ring 511, down-extending tube 512, first aperture 513, second aperture 514, third aperture 515, third spring 516, third sealing ball 517, movable tube 518, collar 519, feed gap 520, middle ring 521, discharge gap 522, extraction section 6, extraction tube 60, cannula 61, screw-on ring 62, inner tube 63, fifth aperture 64, sealing cap 65, seventh aperture 66, fourth sealing ball 67, fourth spring 68, sixth aperture 69, placement mechanism 7, mounting bracket 700, connecting rod 701, rubber head 702, pin 703, first cylinder 704, tapered column 705, connecting rod 706, protrusion 707, lower plate 708, concave frame 709, inner plate 710, rotating plate 711, roller 712, tension spring 713, mounting side plate 714, positioning arc plate 715, upper penetrating plate 716, placing part 8, upper extension stand 80, hose 81, extracting head 82, mounting lower plate 83, constraint ring 84, placing bottle 85, rubber plug 86, liquid taking pipe 87, liquid outlet part 9, lifting screw 90, movable concave piece 91, rotating shaft seat 92, rotating end seat 93, liquid outlet motor 94, rectangular column 95, plug 96 and connecting plug 97.

Description of the embodiments

Referring to fig. 1 and 2, a solid phase extraction apparatus includes an extraction part 1, a gate-type frame 2 is installed on the extraction part 1, a transverse screw 3 is installed at the upper end of the gate-type frame 2, a vertical screw 4 is installed at the output end of the transverse screw 3, a pipetting mechanism 5 is arranged on the vertical screw 4, a plurality of placement mechanisms 7 are further installed on the extraction part 1, an extraction part 6 is placed on the placement mechanism 7, a placement part 8 is arranged on one side of the extraction part 1, and a liquid outlet part 9 is arranged on the other side of the extraction part 1.

In this embodiment, when operating, the operator fills the extraction column or the packing in the extraction part 6, then adds distilled water into the corresponding parts of the extraction part 1, and in addition, places the activator, the rinse, the washing machine and the sample solution at the corresponding positions, then firstly sucks the activator through the pipetting mechanism 5, then transfers the activator to the upper part of one of the extraction parts 6 through the transverse screw 3, then connects the pipetting mechanism 5 with the upper end of the extraction part through the vertical screw 4, then adds the activator, and after the addition, the activator passes through the extraction column or the packing through the extraction part 1, wets the extraction column or the packing and removes impurities therein, the activator can be methanol or the like, and then the activator is discharged through the liquid outlet part 9. Thereafter, the pipes and the related parts in the extraction part are washed with distilled water. And then the transverse screw rod 3 transfers the pipetting mechanism 5 to the corresponding position, samples are extracted by the pipetting mechanism 5, and then sample solutions are sequentially injected into the extraction part 6, so that the extraction column in the extraction part 6 fully adsorbs the target object. The solution is then withdrawn through the withdrawal part 1 and discharged through the liquid outlet part 9. The eluting agent is transferred to the extraction part 6 again by the pipetting mechanism 5, non-target objects are eluted by the eluting agent, and after soaking for a period of time, the non-target objects are extracted by the extraction part 1 and discharged by the liquid outlet part 9, and then repeatedly washed by distilled water. In the cleaning process, the eluent is transferred into the extraction part 6 by the pipetting mechanism 5, the target on the extraction column is soaked and eluted by the eluent, the solution is pumped out by the extraction part 1 after soaking for a period of time and collected, and then the extraction column can be eluted for a plurality of times by the eluent again.

Referring to fig. 2 to 6, the extracting unit 1 includes a bottom plate 100, end plates 101 are respectively disposed on the left and right sides of the bottom plate 100, a cover plate 103 is disposed at the upper end of the end plate 101, side plates 102 are respectively disposed on the front and rear sides of the bottom plate 100, a plurality of extracting mechanisms are mounted on the bottom plate 100, a liquid discharge head 126 is connected to the lower end of each extracting mechanism, a liquid discharge pipe 127 is connected to the liquid discharge head 126, the outer end of the liquid discharge pipe 127 passes through one side plate 102, a fixing plate 142 is mounted on the side plate 102, the liquid discharge pipe 127 passes through the fixing plate 142, a water tank 139 is mounted on the cover plate 103, distilled water is filled in the water tank 139, one end of each extracting mechanism is connected to a liquid inlet head 130, the upper end of each liquid inlet head 130 is connected to a liquid inlet pipe 135, one end of each three-way electromagnetic valve 136 is connected to a first branch pipe 137, the other end of each three-way electromagnetic valve 136 is connected to a second branch pipe 138, and the other end of each second branch pipe 138 passes through the cover plate 103 and is connected to the water tank 139.

Referring to fig. 3 to 6, the drawing mechanism includes a mounting table 104 mounted on a base plate 100, a pair of mounting seats 109 are mounted at one end of the mounting table 104, bearing seats 110 are mounted at the upper ends of the mounting seats 109, a rotating shaft 108 is rotatably provided on the bearing seats 110, one end of the rotating shaft 108 is connected with a motor 107, the motor 107 is mounted on the base plate 100 through a mounting plate 106, a rotating arm 111 is connected on the rotating shaft 108, a connecting shaft 112 is provided at the other end of the rotating arm 111, a connecting arm 113 is rotatably provided on the connecting shaft 112, a connecting seat 114 is rotatably provided at the other end of the connecting arm 113, and a movable rod 115 is mounted on the connecting seat 114.

Referring to fig. 3 to 6, a piston 118 is mounted at the other end of the movable rod 115, a plurality of first sealing rings 123 are provided at the periphery of the piston 118, an end cover 116 is sleeved on the movable rod 115, a sealing ring 117 is provided in the end cover 116, the sealing ring 117 is sleeved on the movable rod 115, a first circular hole 119 is connected to the piston 118, a second circular hole 120 is connected to the inner side end of the first circular hole 119, a third circular hole 121 is connected to the second circular hole 120, the second circular hole 120 and the third circular hole 121 are formed on the movable rod 115, the aperture of the second circular hole 120 is larger than that of the first circular hole 119, a first sealing ball 122 is provided in the second circular hole 120, a first spring 143 is provided in the second circular hole 120, the first sealing ball 122 is located at the outer side end of the first spring 143, the first sealing ball 122 is used for sealing or connecting the first circular hole 119 and the second circular hole 120, a movable housing 124 is mounted on the end cover 116, a movable circular hole 125 is provided in the movable housing 124, the piston 118 moves in the movable circular hole 125, the drain head 126 is screwed to the lower end of the movable housing 124, and the drain head 126 is connected to the movable circular hole 125.

Referring to fig. 3 to 6, a second end cover 128 is mounted at the other end of the movable housing 124, a fourth round hole 129 is formed at the upper end of the second end cover 128 in an opening manner, the liquid inlet head 130 is screwed at the upper end of the fourth round hole 129, a fifth round hole 131 is communicated with the lower end of the fourth round hole 129, the aperture of the fifth round hole 131 is larger than that of the fourth round hole 129, a sealing end is arranged at the lower end of the fifth round hole 131 and is screwed at the lower end of the second end cover 128, a second spring 132 is arranged in the fifth round hole 131, a second sealing ball 133 is arranged at the upper end of the second spring 132, the second sealing ball 133 is located in the fifth round hole 131, the second sealing ball 133 is used for communicating or sealing the fifth round hole 131 with the fourth round hole 129, a sixth round hole 134 is communicated with the lower end of the fifth round hole 131, and the sixth round hole 134 is communicated with the movable round hole 125.

When the extraction mechanism provided in this embodiment works, the motor 107 is started, the rotating shaft 108 is driven by the motor 107 to rotate, the rotating shaft 108 rotates to drive the rotating arm 111 to rotate, the connecting arm 113 drives the movable rod 115 to reciprocate when the rotating arm 111 rotates, and the extraction operation of liquid in the extraction part 6 or distilled water in the water tank 139 is realized through the reciprocation of the movable rod 115.

When the liquid is drawn out, the movable rod 115 drives the piston 118 to move towards the end cover 116, at this time, the liquid is discharged from the liquid discharge head 126 due to the extrusion of the piston 118 to the liquid in the movable round hole 125, and meanwhile, when the liquid is discharged, the first sealing ball 122 seals the first round hole 119 and the second round hole 120. At the same time, the piston 118 will make the piston 118 generate suction force to the second sealing ball 133, so that the second sealing ball 133 is unsealed from the fifth round hole 131 and the fourth round hole 129, and thus, the liquid enters the expanding space of the movable round hole 125 through the liquid inlet head 130, and then the distilled water in the water tank 139 and the solution contained in the extraction part 6 can be pumped out into the movable round hole 125.

When the piston 118 moves toward the second end cap 128, the space of the solution or distilled water is compressed, so that the solution or distilled water in the space is extruded by the first sealing ball 122 to move backward, so that the solution or distilled water enters the expanding space of the movable circular hole 125 through the first circular hole 119, the second circular hole 120 and the third circular hole 121, and meanwhile, the second sealing ball 133 seals the fifth circular hole 131 and the fourth circular hole 129 due to the extrusion force, so that the extraction operation of the solution or distilled water is facilitated.

The other side plate 102 is provided with a liquid outlet 9, and the liquid outlet 9 is communicated with a liquid discharge pipe 127.

The lateral screw 3 can adjust the position of the pipetting mechanism 5 so that the pipetting mechanism 5 transfers the liquid in the placing bottle 85 into the extraction section 6, the first branch pipe 137 is communicated with the liquid inlet pipe 135 through the three-way electromagnetic valve 136, the liquid is conveyed into the liquid outlet section 9 through the liquid outlet pipe 127 through the extraction mechanism and is discharged from the liquid outlet section 9, and after the liquid in the extraction section 6 is discharged, the communication between the first branch pipe 137 and the liquid inlet pipe 135 is closed through the three-way electromagnetic valve 136, and the communication between the second branch pipe 138 and the liquid inlet pipe 135 is opened, so that distilled water washes the inside of the extraction mechanism and the liquid outlet pipe 127.

Referring to fig. 3, a liquid injection head 141 is provided at one end of the upper top of the water tank 139, a liquid injection cover is provided at the upper end of the liquid injection head 141, an air inlet column 140 is provided at the other end of the upper top of the water tank 139, and filter cotton is filled in the air inlet column 140. When the injection of distilled water is performed, the injection cap may be opened and then the addition of distilled water is performed. And when cleaning, the air filtered by the filter cotton enters the water tank 139, so that distilled water is conveniently extracted, distilled water is used for cleaning, and the influence of additional impurities on the extraction purity of the target is reduced.

Referring to fig. 7, a mounting back plate 40 is mounted on the output end of the vertical screw 4, a pair of fixing arc plates 41 are mounted on the mounting back plate 40, and the fixing arc plates 41 are fixed by screws 42.

Referring to fig. 8 and 9, the pipetting mechanism 5 includes a pipette 500 fixed by a fixed arc plate 41, a pipetting head is provided at the lower end of the pipette 500, a cover 501 is screwed to the upper end of the pipette 500, the cover 501 is connected to an upper plate 503 by a plurality of middle rods 502, a pipetting motor 504 is mounted on the upper plate 503, an output shaft of the pipetting motor 504 is connected to a driving gear 505, the driving gear 505 is meshed with a driven gear 506, a wire sleeve 507 is provided on the driven gear 506, the wire sleeve 507 is rotatably provided on the upper plate 503, a pressing screw 508 is screwed in the wire sleeve 507, the pressing screw 508 is threaded on the cover 501, an extruding disc is provided at the lower end of the pressing screw 508, the extruding disc is positioned in the pipette 500, a movable plate 509 is provided at the upper end of the pressing screw 508, a pair of guide rods 510 are provided on the movable plate 509, and the guide rods 510 are threaded on the upper plate 503.

Since the direction of movement of the squeeze pan is different when the liquid is extracted or squeezed, the extraction of the activator, the eluent, the sample solution, and the eluent will be described only as an example. When the liquid-transferring head is drawn, the liquid-transferring motor 504 is started after the liquid-transferring head is inserted into the corresponding position through the vertical screw 4, the driving gear 505 is driven by the liquid-transferring motor 504 to rotate, the driven gear 506 is driven by the rotation of the driving gear 505, the wire sleeve 507 is driven by the rotation of the driven gear 506 to rotate, the lower pressing screw 508 is driven by the rotation of the wire sleeve 507 to move upwards along the length direction of the guide rod 510, and when the liquid-transferring head moves upwards, the pressing plate forms suction force on the liquid, so that the liquid is finally drawn into the pipette 500.

Referring to fig. 10, the pipette head includes a mounting ring 511 mounted at the lower end of the pipette 500, a first aperture 513 is formed in the mounting ring 511, a lower extension tube 512 is disposed at the lower end of the mounting ring 511, a second aperture 514, a third aperture 515 and a fourth aperture are sequentially disposed inside the lower extension tube 512 from top to bottom, the second aperture 514 is communicated with the first aperture 513, the third aperture 515 is communicated with the second aperture 514, the fourth aperture is communicated with the third aperture 515, the aperture of the second aperture 514 is larger than the aperture of the first aperture 513, the aperture of the second aperture 514 is larger than the aperture of the third aperture 515, the aperture of the third aperture 515 is larger than the aperture of the fourth aperture, a third spring 516 is disposed in the second aperture 514, a third sealing ball 517 is disposed at the lower end of the third spring 516, the third sealing ball 517 is used for controlling the closing or communication between the second aperture 514 and the third aperture 515, a movable tube 518 is disposed in the fourth aperture, a convex ring 519 is disposed at the upper end of the movable tube 518, the convex ring 519 is disposed in the third aperture 515, the aperture 514 is larger than the aperture 513 is larger than the aperture of the aperture 513, the aperture is disposed at the upper end of the movable tube 518, the middle portion of the movable tube 518 is provided with a gap 522 is disposed at the lower end of the movable tube 518.

Specifically, when the solution is injected by inserting the movable tube 518 on the sealing cap 65 provided on the sealing member during the pipetting operation, or by inserting the movable tube 518 on the withdrawal head 82 provided on the placement unit 8, the movable tube 518 is inserted in the withdrawal head 82 due to the supporting action of the withdrawal head 82 against the middle ring 521, on the one hand, and the movable tube 518 is moved upward due to the upward pushing force of the movable tube 518, on the other hand, while the third spring 516 is compressed, and the solution inlet notch 520 is located at the lower end of the second eyelet 514, at this time, the movable tube 518 is subjected to the withdrawal force due to the solution being withdrawn. When the solution is extruded, the movable tube 518 pushes the fourth sealing ball 67 arranged on the sealing member downwards, so that the sealing of the upper end of the extraction tube 60 is canceled, and the upper end of the movable tube 518 jacks up the third sealing ball 517 due to the supporting effect of the sealing cover 65 on the centering ring 521, so that the solution can be injected into the extraction tube 60 through the liquid inlet notch 520 and the liquid outlet notch 522 when the solution is extruded.

Referring to fig. 4 and 11, the lower ends of the extraction parts 6 are connected to the first branch pipes 137, the number of the extraction parts 6 is identical to that of the first branch pipes 137, the first branch pipes 137 are in one-to-one correspondence with the extraction parts 6, and the extraction parts 6 are communicated with the first branch pipes 137 corresponding thereto. The extraction part 6 comprises an extraction tube 60, an extraction column is filled at the lower end of the inner part of the extraction tube 60, an insertion tube 61 is arranged at the lower end of the extraction tube 60, and a sealing element is arranged at the upper end of the extraction tube 60.

Referring to fig. 11, the sealing member includes a screw ring 62 screwed to the upper end of the interior of the extraction tube 60, a plurality of air holes are formed in the screw ring 62, an inner tube 63 is disposed in the screw ring 62, a sealing cap 65 is screwed to the upper end of the inner tube 63, a fifth hole 64 is disposed at the lower end of the inner tube 63, a sixth hole 69 is communicated with the upper end of the fifth hole 64, a cavity is formed in the sealing cap 65, the sixth hole 69 is communicated with the cavity, a seventh hole 66 is formed in the upper end of the sealing cap 65, a fourth spring 68 is disposed in the sixth hole 69, a fourth sealing ball 67 is disposed at the upper end of the fourth spring 68, and the fourth sealing ball 67 is used for controlling the communication or sealing between the seventh hole 66 and the cavity. The fifth eyelet 64 is of a conical configuration and has a large end at its upper end. When the liquid of the pipette 500 is squeezed in, the lower end of the movable tube 518 is located in the cavity, and the upper end of the movable tube 518 is located in the second aperture 514, the liquid enters the movable tube 518 from the liquid inlet notch 520, and is discharged from the liquid outlet notch 522 into the sixth aperture 69. The vent holes facilitate the evacuation of air from the extraction tube 60 during the injection of the solution, and the fourth sealing ball 67 can block the seventh aperture 66 from the cavity during the extraction, thereby avoiding the entry of impurities into the solution in the extraction tube 60.

Referring to fig. 3 and 12 to 14, the placement mechanism 7 includes a mounting frame 700 mounted on the cover plate 103, a plurality of rubber heads 702 are provided on the mounting frame 700, the rubber heads 702 are connected to the upper ends of the first branch pipes 137, a plurality of connecting rods 701 are provided on the mounting frame 700, an upper penetrating plate 716 is provided at the upper end of the connecting rods 701, a pair of penetrating pins 703 penetrate through the upper penetrating plate 716, a first cylinder 704 is provided at the lower end of the penetrating pins 703, a tapered column 705 is provided at the lower end of the first cylinder 704, the lower end of the tapered column 705 is a small end, a connecting rod 706 is provided at the lower end of the tapered column 705, a protrusion 707 is provided at the lower end of the connecting rod 706, a lower penetrating plate 708 is provided between the protrusions 707, a pair of concave frames 709 are mounted at the lower wall of the upper penetrating plate 716, an inner extending plate 710 is provided at the lower end of the vertical section of the concave frames 709, a rotating plate 711 is provided at the inner side end of the inner extending plate 710, a roller 712 is provided at the upper end of the rotating plate 711, the outer periphery of the roller 712 is tangential to the tapered column 705 and the first cylinder 704, a tension spring 714 is provided at the lower end of the rotating plate 711, a pair of installing side plates are provided between each pair of installing side plates and each pair of positioning plates are provided with a connecting rod 713, and each pair of the connecting rods 713 are connected.

When the extraction section 6 is placed on the placement mechanism 7, the extraction tube 60 is threaded through the upper threading 716 and the insertion tube 61 is threaded through the lower threading 708. When the roller 712 acts on the first cylinder 704, the positioning arc plate 715 acts on the outer periphery of the extraction tube 60 for limiting the extraction tube 60. When the roller 712 acts on the link 706, the positioning arc plate 715 is in an open state.

When placing the extraction tube 60, the operator wears the extraction tube 60 on the upper threading plate 716 and then presses the extraction tube 60, so that the insertion tube 61 is threaded on the lower threading plate 708 and drives the lower threading plate 708 to move downwards, when the lower threading plate 708 moves downwards, the connecting rod 706, the tapered column 705 and the first column 704 are pulled to move downwards, and when the first column 704 acts on the rollers 712, the distance between the two rollers 712 can be increased, meanwhile, the rotating plate 711 drives the positioning arc plate 715 to rotate inwards, and then the extraction tube 60 is restrained and limited by the positioning arc plate 715, and the tension spring 713 is in a stretched state after being limited.

Referring to fig. 15, at least four placement bottles 85 are provided on the placement unit 8. The placement unit 8 includes a lower mounting plate 83 mounted on a side plate 102, a constraint ring 84 is provided at an outer end of the lower mounting plate 83, and a placement bottle 85 is placed in the constraint ring 84. The upper end of the placing bottle 85 is provided with a rubber plug 86, and a liquid taking pipe 87 and an air permeable head penetrate through the rubber plug 86. An upper extension table 80 is installed at one end of the installation frame 700, at least four hoses 81 are penetrated on the upper extension table 80, an extraction head 82 is arranged at the upper end of each hose 81, and the other ends of the hoses 81 are respectively communicated with a liquid taking pipe 87. In operation, the operator places the placement bottle 85 containing the corresponding solution on the confinement ring 84, and then connects the hose 81 to the liquid take tube 87, followed by a liquid take operation. And the rubber plug 86 can prevent impurities in the air from entering.

Referring to fig. 16 and 17, the liquid outlet 9 includes a lifting screw 90 mounted on a side plate 102, a movable concave member 91 is mounted at an output end of the lifting screw 90, two ends of the movable concave member 91 are respectively provided with a rotating shaft seat 92, a rotating end seat 93 is mounted on one rotating shaft seat 92, a liquid outlet motor 94 is mounted on the rotating end seat 93, an output shaft of the liquid outlet motor 94 is connected with a rectangular column 95, a plurality of plugs 96 are arranged on four side walls of the rectangular column 95, four long holes are formed in the rectangular column 95, the long holes are respectively communicated with the plugs 96 adjacent to the rectangular column 95, one end of each long hole is opened, a connecting plug 97 is arranged at an opening end, and an outer end of a liquid discharge tube 127 penetrates through the plugs 96 and discharges liquid through the connecting plugs 97.

When the solution is discharged, the rectangular column 95 is rotated by the liquid discharge motor 94 so that the spigot 96 on the rectangular column 95 is faced to the liquid discharge tube 127, then the rectangular column 95 is moved upward by the elevating screw 90, and the spigot 96 is communicated with the liquid discharge tube 127, and then the liquid discharge tube 127 discharges the solution from the connection plug 97 through the long hole or into a specified container through a pipe connected to the connection plug 97.

The above description is only of the preferred embodiments of the present invention and is not intended to limit the invention, and it will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (8)

1. A solid phase extractor, comprising:

the extraction part (1) comprises a bottom plate (100), wherein end plates (101) are respectively arranged on the left side and the right side of the bottom plate (100), a cover plate (103) is arranged at the upper end of the end plates (101), side plates (102) are respectively arranged on the front side and the rear side of the bottom plate (100), a plurality of extraction mechanisms are arranged on the bottom plate (100), the lower ends of the extraction mechanisms are connected with liquid discharge heads (126), liquid discharge pipes (127) are connected to the liquid discharge heads (126), the outer side ends of the liquid discharge pipes (127) penetrate through one side plate (102), a fixing plate (142) is arranged on the side plate (102), the liquid discharge pipes (127) penetrate through the fixing plate (142), a water tank (139) is arranged on the cover plate (103), distilled water is filled in the water tank (139), one end of each extraction mechanism is connected with a liquid inlet head (130), the upper ends of each liquid inlet head (130) are communicated with a liquid inlet pipe (135), the upper ends of each liquid inlet pipe (135) are connected with a three-way electromagnetic valve (136), one end of each three-way electromagnetic valve (136) is communicated with a first branch pipe (137), each other, the other end of each three-way electromagnetic valve (136) penetrates through the cover plate (103), the other, and the other end of each three-way electromagnetic valve (136) is communicated with the other is communicated with the water tank (139), and is communicated with the other through the other (139).

The cover plate (103) is provided with a door-shaped frame (2);

the upper end of the door-shaped frame (2) is provided with a transverse screw rod (3);

the output end of the transverse screw rod (3) is provided with a vertical screw rod (4);

a pipetting mechanism (5) is arranged on the vertical screw rod (4);

a plurality of placement mechanisms (7) are arranged on the cover plate (103);

the extraction parts (6) are arranged on the placing mechanism (7), the lower ends of the extraction parts (6) are connected to the first branch pipes (137), the number of the extraction parts (6) is consistent with that of the first branch pipes (137), the first branch pipes (137) are in one-to-one correspondence with the extraction parts (6), and the extraction parts (6) are communicated with the first branch pipes (137) corresponding to the extraction parts;

one side plate (102) is provided with a placing part (8), and the placing part (8) is provided with at least four placing bottles (85);

the other side plate (102) is provided with a liquid outlet part (9), and the liquid outlet part (9) is communicated with a liquid discharge pipe (127);

the transverse screw rod (3) is used for adjusting the position of the liquid transferring mechanism (5) so that the liquid in the placing bottle (85) is transferred into the extraction part (6) by the liquid transferring mechanism (5), the first branch pipe (137) is communicated with the liquid inlet pipe (135) through the three-way electromagnetic valve (136), the liquid is conveyed into the liquid outlet part (9) through the liquid outlet pipe (127) through the extraction mechanism and is discharged from the liquid outlet part (9), and after the liquid in the extraction part (6) is discharged, the communication between the first branch pipe (137) and the liquid inlet pipe (135) is closed through the three-way electromagnetic valve (136), and the communication between the second branch pipe (138) and the liquid inlet pipe (135) is opened so that distilled water washes the inside of the extraction mechanism and the liquid outlet pipe (127);

The extraction mechanism comprises a mounting table (104) mounted on the bottom plate (100), a pair of mounting seats (109) are mounted at one end of the mounting table (104), bearing seats (110) are mounted at the upper ends of the mounting seats (109) respectively, rotating shafts (108) are rotatably arranged on the bearing seats (110), one ends of the rotating shafts (108) are connected with motors (107), the motors (107) are mounted on the bottom plate (100) through mounting plates (106), rotating arms (111) are connected to the rotating shafts (108), connecting shafts (112) are arranged at the other ends of the rotating arms (111), connecting arms (113) are rotatably arranged on the connecting shafts (112), connecting seats (114) are rotatably arranged at the other ends of the connecting arms (113), and movable rods (115) are mounted on the connecting seats (114);

the other end of the movable rod (115) is provided with a piston (118), the periphery of the piston (118) is provided with a plurality of first sealing rings (123), the movable rod (115) is sleeved with an end cover (116), the end cover (116) is internally provided with a sealing ring (117), the sealing ring (117) is sleeved on the movable rod (115), the piston (118) is communicated with a first round hole (119), the inner side end of the first round hole (119) is communicated with a second round hole (120), the second round hole (120) is communicated with a third round hole (121), the second round hole (120) and the third round hole (121) are formed on the movable rod (115), the aperture of the second round hole (120) is larger than the aperture of the first round hole (119), a first sealing ball (122) is arranged in the second round hole (120), a first spring (143) is arranged in the second round hole (120), the first sealing ball (122) is positioned at the outer side end of the first spring (143), the first sealing ball (122) is used for the inner side end or the communication of the first round hole (119) and the second round hole (120), the end cover (116) is communicated with the third round hole (121), the movable shell (124) is arranged on the end cover (116), the movable shell (124) is arranged in the movable shell (124) and is connected with the movable shell (125) in the movable shell (125), the liquid discharge head (126) is communicated with the movable round hole (125);

The other end of the movable shell (124) is provided with a second end cover (128), a fourth round hole (129) is formed in an opening way at the upper end of the second end cover (128), the liquid inlet head (130) is in threaded connection with the upper end of the fourth round hole (129), the lower end of the fourth round hole (129) is communicated with a fifth round hole (131), the aperture of the fifth round hole (131) is larger than that of the fourth round hole (129), the lower end of the fifth round hole (131) is provided with a sealing end head, the sealing end is in threaded connection with the lower end of the second end cover (128), a second spring (132) is arranged in the fifth round hole (131), a second sealing ball (133) is arranged at the upper end of the second spring (132), the second sealing ball (133) is positioned in the fifth round hole (131), the second sealing ball (133) is used for communicating or sealing the fifth round hole (131) with the fourth round hole (129), the lower end of the fifth round hole (131) is communicated with a sixth round hole (134), and the sixth round hole (134) is communicated with the movable round hole (125);

the output end of the vertical screw rod (4) is provided with a mounting backboard (40), the mounting backboard (40) is provided with a pair of fixing arc plates (41), and the fixing arc plates (41) are fixed through screws (42);

pipetting mechanism (5) including pipette (500) fixed through fixed arc board (41), the lower extreme of pipette (500) is equipped with the pipette head, the upper end spiro union of pipette (500) has lid (501), lid (501) are connected with upper plate (503) through many middle poles (502), install pipetting motor (504) on upper plate (503), the output shaft of pipetting motor (504) has driving gear (505), driving gear (505) meshing has driven gear (506), be equipped with silk cover (507) on driven gear (506), silk cover (507) rotate locate on upper plate (503), the spiro union has down lead screw (508) in silk cover (507), down press lead screw (508) wear on lid (501), the lower extreme of pushing down lead screw (508) is equipped with extrudees pressure disk, it is located pipette (500) to push down the upper end of lead screw (508) and is equipped with fly leaf (509), be equipped with a pair of guide bar (510) on fly leaf (509), guide bar (510) wear on upper plate (503).

2. The solid phase extraction instrument according to claim 1, wherein a liquid injection head (141) is arranged at one end of the upper top of the water tank (139), a liquid injection cover is arranged at the upper end of the liquid injection head (141), an air inlet column (140) is arranged at the other end of the upper top of the water tank (139), and filter cotton is filled in the air inlet column (140).

3. The solid phase extraction apparatus according to claim 1, wherein the extraction section (6) comprises an extraction tube (60), an extraction column is filled in the lower end of the extraction tube (60), an insertion tube (61) is provided in the lower end of the extraction tube (60), and a sealing member is provided in the upper end of the extraction tube (60).

4. The solid phase extraction apparatus according to claim 3, wherein the pipette head comprises a mounting ring (511) mounted at the lower end of the pipette (500), the mounting ring (511) is provided with a first hole (513), the lower end of the mounting ring (511) is provided with a lower extension tube (512), a second hole (514), a third hole (515) and a fourth hole are sequentially arranged in the lower extension tube (512) from top to bottom, the second hole (514) is communicated with the first hole (513), the third hole (515) is communicated with the second hole (514), the fourth hole is communicated with the third hole (515), the aperture of the second hole (514) is larger than the aperture of the first hole (513), the aperture of the second hole (514) is larger than the aperture of the third hole (515), the aperture of the third hole (515) is larger than the aperture of the fourth hole, a third spring (516) is arranged in the second hole (514), the lower end of the third spring (516) is provided with a third sealing ball (517), the third sealing ball (517) is used for controlling the second hole (514) to be communicated with the first hole (513), the aperture of the third hole (515) is provided with a bulge (519), the upper end of the movable tube (519) is arranged in the third hole (518), the middle part of the periphery of the movable pipe (518) is provided with a middle ring (521), and the lower end of the movable pipe (518) is provided with a liquid outlet notch (522).

5. The solid phase extraction instrument according to claim 4, wherein the sealing member comprises a screw ring (62) screwed at the upper end inside the extraction tube (60), a plurality of air holes are formed in the screw ring (62), an inner tube (63) is arranged in the screw ring (62), a sealing cover (65) is screwed at the upper end of the inner tube (63), a fifth eyelet (64) is arranged at the lower end of the inner tube (63), a sixth eyelet (69) is communicated at the upper end of the fifth eyelet (64), a cavity is formed in the sealing cover (65), the sixth eyelet (69) is communicated with the cavity, a seventh eyelet (66) is formed at the upper end of the sealing cover (65), a fourth spring (68) is arranged in the sixth eyelet (69), a fourth sealing ball (67) is arranged at the upper end of the fourth spring (68), and the fourth sealing ball (67) is used for controlling the communication or sealing between the seventh eyelet (66) and the cavity;

the fifth eyelet (64) has a conical structure and a large end at the upper end;

when the liquid of the pipette (500) is squeezed in, the lower end of the movable tube (518) is positioned in the cavity, the upper end of the movable tube (518) is positioned in the second hole (514), the liquid enters the movable tube (518) from the liquid inlet notch (520), and is discharged from the liquid outlet notch (522) into the sixth hole (69).

6. The solid phase extraction apparatus according to claim 3, wherein the placement mechanism (7) comprises a mounting frame (700) mounted on the cover plate (103), a plurality of rubber heads (702) are arranged on the mounting frame (700), the rubber heads (702) are connected to the upper ends of the first branch pipes (137), a plurality of connecting rods (701) are mounted on the mounting frame (700), an upper penetrating plate (716) is mounted at the upper ends of the connecting rods (701), a pair of penetrating pins (703) are penetrated on the upper penetrating plate (716), a first cylinder (704) is arranged at the lower ends of the penetrating pins (703), a conical column (705) is arranged at the lower ends of the first cylinder (704), the lower ends of the conical column (705) are small ends, connecting rods (706) are arranged at the lower ends of the conical column (705), protrusions (707) are arranged at the lower ends of the connecting rods (706), a lower penetrating plate (708) is arranged between the protrusions (707), a pair of concave frames (709) are mounted on the lower walls of the upper penetrating plate (716), an inner extending plate (710) is arranged at the lower ends of the vertical section of the concave frames, the inner extending plate (710) is provided with a rotating plate, the rotating plate (710) is provided with a pair of penetrating pins (703), the rotating plate (711) is provided with a first round roller (706), the upper end (711) is provided with a round roller (712), and the rotating plate (711) is provided with a round roller (712), a positioning arc plate (715) is arranged between each pair of mounting side plates (714), connecting pins are arranged on the inner wall of the rotating plate (711), and a tension spring (713) is arranged between each pair of connecting pins positioned on the same side;

When the extraction part (6) is placed on the placement mechanism (7), the extraction tube (60) penetrates through the upper penetrating plate (716), and the insertion tube (61) penetrates through the lower penetrating plate (708);

when the roller (712) acts on the first cylinder (704), the positioning arc plate (715) acts on the periphery of the extraction tube (60) for limiting the extraction tube (60);

when the roller (712) acts on the connecting rod (706), the positioning arc plate (715) is in an open state.

7. The solid phase extraction apparatus according to claim 6, wherein the placement section (8) includes a mounting lower plate (83) mounted on the side plate (102), a restriction ring (84) is provided at an outer side end of the mounting lower plate (83), and the placement bottle (85) is placed in the restriction ring (84);

a rubber plug (86) is arranged at the upper end of the placing bottle (85), and a liquid taking pipe (87) and a ventilation head are penetrated on the rubber plug (86);

an upper extension table (80) is arranged at one end of the mounting frame (700), at least three hoses (81) are penetrated on the upper extension table (80), extraction heads (82) are arranged at the upper ends of the hoses (81), and the other ends of the hoses (81) are respectively communicated with a liquid taking pipe (87).

8. The solid phase extraction instrument according to claim 1, wherein the liquid outlet portion (9) comprises a lifting screw (90) mounted on a side plate (102), a movable concave piece (91) is mounted at an output end of the lifting screw (90), rotating shaft seats (92) are respectively arranged at two ends of the movable concave piece (91), a rotating end seat (93) is mounted on one rotating shaft seat (92), a liquid outlet motor (94) is mounted on the rotating end seat (93), a rectangular column (95) is connected to an output shaft of the liquid outlet motor (94), a plurality of inserting nozzles (96) are arranged on four side walls of the rectangular column (95), four long holes are formed in the rectangular column (95), the long holes are respectively communicated with the inserting nozzles (96) adjacent to the rectangular column, one end of each long hole is opened, a connecting plug (97) is arranged at an opening end, and an outer side end of a liquid discharging pipe (127) penetrates through the inserting nozzles (96) and discharges liquid through the connecting plug (97).