CN116602311A - Bactericide for plant growth period and application thereof - Google Patents

Bactericide for plant growth period and application thereof Download PDF

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Publication number
CN116602311A
CN116602311A CN202310583666.3A CN202310583666A CN116602311A CN 116602311 A CN116602311 A CN 116602311A CN 202310583666 A CN202310583666 A CN 202310583666A CN 116602311 A CN116602311 A CN 116602311A
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metalaxyl
bactericide
agent
plant growth
plant
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梁召俊
曾宪峰
解勇
高乾奎
黄贤喜
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Qingdao Hansen Biologic Science Co ltd
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Qingdao Hansen Biologic Science Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a bactericide for a plant growing period and application thereof, and belongs to the field of agricultural bactericides. The bactericide for the plant growth period provided by the invention consists of an active component and an auxiliary material, wherein the active component consists of tetrazole picoline and metalaxyl-M, and the weight ratio of the active component to the metalaxyl-M is 50:1-1:50, preferably 1:10-10:1. The bactericide for plant growth period provided by the invention takes the tetrazolium picolinate and the metalaxyl-M as active components, and the two components are synergistic, so that the bactericide can be used for soil-borne diseases caused by multiple strains (fusarium and phytophthora) such as underground root rot and epidemic disease, has unique bactericidal spectrum and rapid action, reduces the residual quantity of pesticides on crops, reduces the harm to the environment, and can effectively delay the generation of drug resistance of pathogenic bacteria.

Description

Bactericide for plant growth period and application thereof
Technical Field
The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericide for plant growth period and application thereof.
Background
Tetrazolium picolinate (picrbutanzox), CAS:500207-04-5; the chemical name is [6- [ [ [ [ (z) - (1-methyl-1H-tetrazol-5-yl) (phenyl) methylene ] amino ] oxy ] methyl ] pyridin-2-yl ] carbamic acid tert-butyl ester, which was found by Dai Japanese ink chemical Co., ltd, and later marketed by Cao da, japan, whose mechanism of action was unknown, and which was classified into the U17 class by the International antiseptic resistance action Commission (FRAC).
Studies have demonstrated that tetrazolium picolinate does not act by inhibiting respiratory chain electron transfer system complexes I and III. By treating Pythium aphanidermatum (Pythium aphanidermatum) with tetrazolium, hyphae become thicker and branch more, it is inferred that the Pythium aphanidermatum has the effects of inhibiting hyphae growth and spore germination, reducing sporangium formation and zoospore quantity, and achieving sterilization. The tetrazolium picolinate has no cross resistance with carboxylic acid amides, phenyl amides and methoxy acrylic acid ester bactericides which are widely used at present, so that the tetrazolium picolinate has good control effects on downy mildew, epidemic diseases, soil-borne stem rot, root rot and other diseases.
metalaxyl-M, the chemical name of which is N- (2, 6-xylyl) -N- (methoxyacetyl) -D-propylamine, is a high-efficiency, low-toxicity, low-residue and systemic bactericide of phenylamide, belongs to a synthesis inhibitor of ribosomal RNA, has strong systemic and osmotic power, can conduct up and down in plants within 30 minutes after application, has protection and treatment effects on disease plants, has long drug effect duration, and mainly inhibits the synthesis of protein in germ mycelium, so that the germ mycelium is nutritional deficiency and cannot grow normally and die. The metalaxyl-M can be used for stem and leaf treatment, seed treatment and soil treatment, and has remarkable control effect on soil-borne crop diseases caused by phytophthora, saprophytis and the like.
The bactericide variety containing single active component has the defect of different degrees in agricultural bactericide control, and can achieve the aim of effectively controlling crop pathogenic bacteria if different components with synergistic effect are reasonably compounded. The pesticide compound can reduce the drug resistance of plant pathogenic bacteria, improve the control effect, enlarge the control range, reduce the dosage of active ingredients, save the medication cost and reduce the environmental pollution, so that the research and development of the efficient low-toxicity pesticide has positive effects on the sustainable development of agriculture for the environment-friendly bactericide in the plant growth period.
Disclosure of Invention
The invention provides a bactericide for plant growth period and application thereof, the bactericide for plant growth period takes tetrazolium picolinate and metalaxyl-M as active components, and the two components are synergistic, can be used for diseases caused by multiple strains (fusarium and phytophthora) such as soil-borne underground root rot, epidemic disease and the like, has unique bactericidal spectrum and rapid action, reduces the residual quantity of pesticides on crops, lightens the harm to the environment, and can effectively delay the generation of drug resistance of pathogenic bacteria.
In order to achieve the aim, the invention provides a bactericide for plant growth period, which consists of an active component and an auxiliary material, wherein the active component consists of tetrazolium picolinate and metalaxyl-M, and the weight ratio of the active component to the metalaxyl-M is 50:1-1:50, preferably 1:10-10:1. The active ingredients are all bactericides well known in the art and are commercially available from a variety of sources.
In the above scheme, in order to ensure good sterilization effect and synergistic effect of the compound plant growth phase bactericide, the preferable weight ratio of the tetrazolium picolinate to the metalaxyl-M is 50:1, 10:1,5:1,1:1,1:3,1:4,1:5,1:10,1:50. It will be appreciated that, depending on the choice of the concentration of the active ingredient during use, the ratio of the tetrazolium picolinate to the metalaxyl may be selected within any numerical ratio within the weight ratio range as desired by the person skilled in the art.
Preferably, the addition amount of the active component is 10% -80% of the total mass of the composition.
Preferably, the auxiliary materials comprise a carrier and an auxiliary agent;
the carrier is selected from at least one of water, solvent or filler;
the auxiliary agent comprises a surfactant or a surfactant and a functional auxiliary agent.
In the above scheme, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate.
Preferably, the surfactant is at least one selected from the group consisting of an emulsifier, a dispersant, a wetting agent and a penetrating agent, and may be nonionic or anionic, or may be a combination of both types.
In the above scheme, the emulsifier is selected from one or a mixture of more of triphenylethyl polyoxyethylene ether phosphate triethanolamine salt, 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether disulfonate magnesium salt, triethanolamine salt, agro-emulsion 400# (benzyl dimethyl phenol polyoxyethylene ether), ningemulsion 36# (phenethyl phenol formaldehyde resin polyoxyethylene ether), agro-emulsion 1600# (phenethyl phenol polyoxyethylene polypropylene ether), block polyether, BY series (castor oil polyoxyethylene ether), agro-emulsion 33# (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitan monostearate), tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether).
The dispersing agent is selected from one or more of polycarboxylate, lignosulfonate, alkylphenol ethoxylate formaldehyde condensate sulfate, calcium alkylbenzenesulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, alkylphenol ethoxylate, fatty amine ethoxylate, fatty acid ethoxylate or glycerin fatty acid ester ethoxylate.
The wetting agent is selected from one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrating agent F, fructus Gleditsiae Abnormalis powder, faeces Bombycis or soapberry powder.
The penetrating agent is selected from one or more of penetrating agent JFC (fatty alcohol polyoxyethylene ether), penetrating agent T (diisooctyl maleate sulfonate), azone and organic silicon.
Preferably, according to different application occasions and requirements, other functional auxiliary agents such as an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent, a defoaming agent and the like can be added into the composite bactericide.
In the above scheme, the thickener is selected from one or more of gelatin, sodium carboxymethyl cellulose, hydroxyethyl cellulose, sodium polyacrylate, modified starch, xanthan gum, bentonite, silicon dioxide and magnesium aluminum silicate.
The antifreezing agent is selected from one or a mixture of more of ethylene glycol, propylene glycol, glycerol or urea.
The defoamer is selected from one or a mixture of more of silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds or C8-C10 fatty alcohol compounds.
The disintegrating agent is selected from one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate.
The stabilizer is one or more selected from epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate.
Preferably, the bactericide in the plant growth period is processed into any formulation suitable for agriculture by being matched with processing auxiliary components of a pesticide preparation.
Preferably, the dosage form is at least one selected from the group consisting of a suspoemulsion, a microemulsion, an aqueous emulsion, a wettable powder, a water dispersible granule and an emulsifiable concentrate.
The invention also provides application of the plant growth period bactericide according to the technical scheme in preventing and treating diseases caused by harmful pathogenic fungi of root rot, fusarium and phytophthora.
Preferably, the harmful pathogenic bacteria are selected from at least one of phytophthora parasitica, phytophthora capsici, eggplant root rot pathogenic bacteria, capsicum root rot pathogenic bacteria, especially at least one of phytophthora parasitica, acanthocethereof, fusarium solani and fusarium solani.
Preferably, the plant is a solanaceous plant selected from capsicum, eggplant or tomato or another crop of peanut, maize.
Compared with the prior art, the invention has the advantages and positive effects that:
1. the bactericide for the plant growth period provided by the invention takes the tetrazolium picolinate and the metalaxyl-M as active components, and the two components are synergistic, so that the bactericide can be used for treating diseases caused by multiple strains (fusarium and phytophthora) such as soil-borne underground root rot of plants of the solanaceae such as capsicum, eggplant and tomatoes and other crops such as peanuts and corns, and particularly has obvious synergistic effect on the prevention and treatment of the root rot of the solanaceae;
2. the bactericide for the plant growth period has unique bactericidal spectrum, quick action, reduced pesticide dosage, reduced pesticide residue on crops, reduced harm to the environment and effective delay of the generation of pathogen resistance.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. In the embodiment, the percentages are weight percentages, and the treatment dosage of the field efficacy is the dosage of the active ingredients.
Example 1 30% Water dispersible granule of tetrazolium picolinate and metalaxyl-M
Proportioning: 15% of tetrazolium picolinate, 15% of metalaxyl-M, 5% of sodium lignin sulfonate, 5% of polyvinyl acetate, 10% of ammonium sulfate and 100% of kaolin.
EXAMPLE 2 24% Methylflupyrad-Methylflufenoxad suspension emulsion
Proportioning: 20% of tetrazolium picolinate, 4% of metalaxyl-M, 2% of sodium dodecyl sulfonate, 2% of sodium lignin sulfonate, 2% of castor oil polyoxyethylene ether, 0.2% of carboxymethyl cellulose, 0.1% of magnesium aluminum silicate, 0.1% of defoamer and 100% of water.
EXAMPLE 3 24% suspension of tetrazolium-mefenoxam
Proportioning: 4% of tetrazolium picolinate, 20% of metalaxyl-M, 2% of sodium dodecyl sulfate, 2% of sodium lignin sulfonate, 4% of triethanolamine salt of triphenyl ethyl polyoxyethylene ether phosphate, 0.2% of carboxymethyl cellulose, 0.1% of magnesium aluminum silicate, 0.1% of defoaming agent and 100% of water.
Example 4 Water dispersible granule of 51% tetrazolium picolinate and metalaxyl-M
Proportioning: 50% of tetrazolium picolinate, 1% of metalaxyl-M, 3% of sodium lignin sulfonate, 3% of polyvinyl acetate, 5% of ammonium sulfate and 100% of kaolin.
Example 5% Water dispersible granule of tetrazolium picolinate and metalaxyl-M
Proportioning: 1% of tetrazolium picolinate, 50% of metalaxyl-M, 3% of sodium lignin sulfonate, 3% of polyvinyl acetate, 5% of ammonium sulfate and 100% of kaolin.
EXAMPLE 6 11% suspension of tetrazolium-mefenoxam
Proportioning: 1% of tetrazolium picolinate, 10% of metalaxyl-M, 2% of sodium dodecyl sulfonate, 2% of sodium lignin sulfonate, 3% of block polyether, 0.2% of carboxymethyl cellulose, 0.1% of magnesium aluminum silicate, 0.1% of defoamer and 100% of water.
EXAMPLE 7 11% suspension of tetrazolium picolinate and metalaxyl-M
Proportioning: 10% of tetrazolium picolinate, 1% of metalaxyl-M, 2% of sodium dodecyl sulfonate, 2% of sodium lignin sulfonate, 2% of castor oil polyoxyethylene ether, 0.2% of carboxymethyl cellulose, 0.1% of magnesium aluminum silicate, 0.1% of defoamer and 100% of water.
EXAMPLE 8 21% Methylflupyrad-Methylflufenoxad suspension emulsion
Proportioning: 20% of tetrazolium picolinate, 1% of metalaxyl-M, 2% of sodium dodecyl sulfonate, 2% of sodium lignin sulfonate, 2% of castor oil polyoxyethylene ether, 0.2% of carboxymethyl cellulose, 0.1% of magnesium aluminum silicate, 0.1% of defoamer and 100% of water.
EXAMPLE 9 21% Methylflupyrad-Methylflufenoxad suspension emulsion
Proportioning: 1% of tetrazolium picolinate, 20% of metalaxyl-M, 2% of sodium dodecyl sulfate, 2% of sodium lignin sulfonate, 4% of triethanolamine salt of triphenyl ethyl polyoxyethylene ether phosphate, 0.2% of carboxymethyl cellulose, 0.1% of magnesium aluminum silicate, 0.1% of defoaming agent and 100% of water.
The preparation methods of example 2, example 3, example 6, example 7, example 8 and example 9 are as follows: according to the proportion, the effective components of the tetrazole ammonia ester, the metalaxyl-M, the surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, high-speed shearing and wet sanding are carried out, and finally, the product is obtained through homogenizing and filtering. The main equipment is a batching kettle, a colloid mill or a homogenizing mixer and a sand mill.
The preparation methods of example 1, example 4 and example 5 are as follows: adding the effective components of the tetrazole ammonia ester and the metalaxyl into a carrier according to the proportion, adding a surfactant and other functional auxiliary agents into the carrier, mixing, carrying out jet milling on the mixture, then carrying out kneading, granulating, drying and screening on the mixture to obtain a water dispersible granule product; or spraying water, granulating, drying, and sieving to obtain the final product. The main equipment includes mixer, jet mill, kneader, extrusion granulator, drying room or fluidized bed, or boiling granulator, sieving machine.
Indoor toxicity measurement
An indoor combined toxicity biological assay test is adopted to verify whether the effective components of the tetrazolium picolinate and the metalaxyl-M have a synergistic effect after being compounded.
Experiment one: indoor virulence determination for phytophthora infestans (Phytophthora infestans (Mont.) DeBary) a tomato late blight pathogen
Test agent: 97% of tetrazolium picolinate original medicine and 91% of metalaxyl-M original medicine
Test target: phytophthora (Phytophthora infestans (Mont.) DeBary) for screening tomato late blight collected in Shandong Shou City
The experimental method comprises the following steps: by inhibiting hypha growth rate
Dissolving tetrazolium picolinate and metalaxyl-M with acetone respectively, diluting with 0.1% Tween-80 aqueous solution, preparing into single-dose mother solution, setting multiple groups of proportions, setting 5 mass concentration gradients of each single dose and proportion mixture according to an equal proportion method, and preparing all medicaments at present. Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, fully shaking uniformly, pouring into 4 culture dishes with the diameter of 9 cm in equal quantity, preparing a medicine-containing flat plate with the relative concentration, setting blank treatment control, and setting 3 repetitions for each treatment. Cutting out bacterial cakes with the diameter of 5 mm from the colony edge of the strain to be tested by using a puncher with the diameter of 5 mm, inoculating the bacterial cakes to the centers of a medicine-containing flat plate and a blank control flat plate, covering a dish cover, placing the dish cover in a 25-DEG incubator for culturing for 4d, measuring the colony diameter by using a crisscross method, and calculating the growth inhibition rate of different treatments on hyphae.
Data analysis: the principle of least square method is applied, DPS software is adopted to conduct data analysis, linear regression is conducted with the value of the disinfectant concentration pair being x and the value of the corresponding hypha growth inhibition rate pair being y, and a virulence regression equation and the concentration EC in the inhibition of the drug to target bacteria are obtained 50 . Co-toxicity coefficients (CTCs) were calculated according to the grand cloud-Pei method.
Measured virulence index (ATI) = (standard agent EC 50 Test agent EC 50 )×100。
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture.
Co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/theoretical drug susceptibility index (TTI) ] x 100.
According to co-toxicity coefficient (CTC) evaluation criteria: CTC is less than or equal to 80 and antagonism, CTC is less than 80 and 120 and additive effect, CTC is more than or equal to 120 and synergistic effect, and the results are shown in Table 1.
Table 1 for tomato late blight pathogen: indoor virulence assay of Phytophthora (Phytophthora infestans (Mont.) DeBary)
As can be seen from Table 1, when the combination of the tetrazolium picolinate and the metalaxyl-M is in the range of 50:1-1:50, the co-toxicity coefficients of the combination of the tetrazolium picolinate and the metalaxyl-M to phytophthora parasitica (Phytophthora infestans (Mont.) DeBary) are all larger than 120, and the combination has obvious synergistic effect.
Experiment II: for pathogenic bacteria of brown root rot of tomatoes: indoor virulence determination of tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Gerlach)
Test agent: 97% of tetrazolium picolinate original medicine and 91% of metalaxyl-M original medicine
Test target: tomato echinococcosis of tomato brown root rot collected from the Shandong Shou City (Pyrenochaeta lycopersici Schneider et Gerlach).
The experimental method comprises the following steps: by inhibiting hypha growth rate
Dissolving tetrazolium picolinate and metalaxyl-M with acetone respectively, diluting with 0.1% Tween-80 aqueous solution, preparing into single-dose mother solution, setting multiple groups of proportions, setting 5 mass concentration gradients of each single dose and proportion mixture according to an equal proportion method, and preparing all medicaments at present. Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, fully shaking uniformly, pouring into 4 culture dishes with the diameter of 9 cm in equal quantity, preparing a medicine-containing flat plate with the relative concentration, setting blank treatment control, and setting 3 repetitions for each treatment. Cutting out bacterial cakes with the diameter of 5 mm from the colony edge of the strain to be tested by using a puncher with the diameter of 5 mm, inoculating the bacterial cakes to the centers of a medicine-containing flat plate and a blank control flat plate, covering a dish cover, placing the dish cover in a 25-DEG incubator for culturing for 4d, measuring the colony diameter by using a crisscross method, and calculating the growth inhibition rate of different treatments on hyphae.
Data analysis: the principle of least square method is applied, DPS software is adopted to conduct data analysis, linear regression is conducted with the value of the disinfectant concentration pair being x and the value of the corresponding hypha growth inhibition rate pair being y, and a virulence regression equation and the concentration EC in the inhibition of the drug to target bacteria are obtained 50 . Co-toxicity coefficients (CTCs) were calculated according to the grand cloud-Pei method.
Measured virulence index (ATI) = (standard agent EC 50 Test agent EC 50 )×100。
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture.
Co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/theoretical drug susceptibility index (TTI) ] x 100.
According to co-toxicity coefficient (CTC) evaluation criteria: CTC.ltoreq.80 is antagonistic, CTC.ltoreq.120 is additive, CTC.ltoreq.120 is synergistic, and the results are shown in Table 2.
Table 2 against tomato brown root rot pathogen: indoor virulence determination of tomato aschersonia (Pyrenochaeta lycopersici Schneider et Gerlach)
From Table 2, it is clear that the combination of tetrazolium picolinate and metalaxyl-M is effective against tomato brown root rot pathogens in the range of 50:1-1:50: the co-toxicity coefficients of the tomato and the aschersonia aleyrodis (Pyrenochaeta lycopersici Schneider et Gerlach) are all more than 120, and the obvious synergistic effect is achieved.
Experiment III: the tetrazolium picolinate and the metalaxyl-M are mixed to the pathogenic bacteria of the eggplant root rot: indoor virulence assay of Fusarium solani (mart.) sacc.)
Test agent: 97% of tetrazolium picolinate original medicine and 91% of metalaxyl-M original medicine
Test target: fusarium solani (Mart.) Sacc.
The experimental method comprises the following steps: by inhibiting hypha growth rate
Dissolving tetrazolium picolinate and metalaxyl-M with acetone respectively, diluting with 0.1% Tween-80 aqueous solution, preparing into single-dose mother solution, setting multiple groups of proportions, setting 5 mass concentration gradients of each single dose and proportion mixture according to an equal proportion method, and preparing all medicaments at present. Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, fully shaking uniformly, pouring into 4 culture dishes with the diameter of 9 cm in equal quantity, preparing a medicine-containing flat plate with the relative concentration, setting blank treatment control, and setting 3 repetitions for each treatment. Cutting out bacterial cakes with the diameter of 5 mm from the colony edge of the strain to be tested by using a puncher with the diameter of 5 mm, inoculating the bacterial cakes to the centers of a medicine-containing flat plate and a blank control flat plate, covering a dish cover, placing the dish cover in a 25-DEG incubator for culturing for 4d, measuring the colony diameter by using a crisscross method, and calculating the growth inhibition rate of different treatments on hyphae.
Data analysis: the principle of least square method is applied, DPS software is adopted to conduct data analysis, linear regression is conducted with the value of the disinfectant concentration pair being x and the value of the corresponding hypha growth inhibition rate pair being y, and a virulence regression equation and the concentration EC in the inhibition of the drug to target bacteria are obtained 50 . Co-toxicity coefficients (CTCs) were calculated according to the grand cloud-Pei method.
Measured virulence index (ATI) = (standard agent EC 50 Test agent EC 50 )×100。
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture.
Co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/theoretical drug susceptibility index (TTI) ] x 100.
According to co-toxicity coefficient (CTC) evaluation criteria: CTC.ltoreq.80 is antagonistic, CTC.ltoreq.120 is additive, CTC.ltoreq.120 is synergistic, and the results are shown in Table 3.
Table 3 pathogen for eggplant root rot: indoor virulence assay of Fusarium solani (mart.) sacc.)
From Table 3, it is clear that the combination of tetrazolium picolinate and metalaxyl-M is effective against eggplant root rot pathogens when the ratio is in the range of 50:1-1:50: fusarium solani (Mart.) Sacc has co-toxicity coefficient greater than 120, and has obvious synergistic effect.
Experiment IV: pathogenic bacteria for screening the root rot of the capsicum: fusarium solani: indoor virulence assay of (Fusarium solani (Mart.)) App.Et Wollenw
Test agent: 97% of tetrazolium picolinate original medicine and 91% of metalaxyl-M original medicine
Test target: pathogenic bacteria Fusarium solani screened from root rot of capsicum collected in shen county of Shandong province: (Fusarium solani (Mart.) App. Et Wollenw).
The experimental method comprises the following steps: by inhibiting hypha growth rate
Dissolving tetrazolium picolinate and metalaxyl-M with acetone respectively, diluting with 0.1% Tween-80 aqueous solution, preparing into single-dose mother solution, setting multiple groups of proportions, setting 5 mass concentration gradients of each single dose and proportion mixture according to an equal proportion method, and preparing all medicaments at present. Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, fully shaking uniformly, pouring into 4 culture dishes with the diameter of 9 cm in equal quantity, preparing a medicine-containing flat plate with the relative concentration, setting blank treatment control, and setting 3 repetitions for each treatment. Cutting out bacterial cakes with the diameter of 5 mm from the colony edge of the strain to be tested by using a puncher with the diameter of 5 mm, inoculating the bacterial cakes to the centers of a medicine-containing flat plate and a blank control flat plate, covering a dish cover, placing the dish cover in a 25-DEG incubator for culturing for 4d, measuring the colony diameter by using a crisscross method, and calculating the growth inhibition rate of different treatments on hyphae.
Data analysis: the principle of least square method is applied, DPS software is adopted to conduct data analysis, linear regression is conducted with the value of the disinfectant concentration pair being x and the value of the corresponding hypha growth inhibition rate pair being y, and a virulence regression equation and the concentration EC in the inhibition of the drug to target bacteria are obtained 50 . Co-toxicity coefficient (CTC) was calculated according to the grand cloud Pei method)。
Measured virulence index (ATI) = (standard agent EC 50 Test agent EC 50 )×100。
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture.
Co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/theoretical drug susceptibility index (TTI) ] x 100.
According to co-toxicity coefficient (CTC) evaluation criteria: CTC.ltoreq.80 is antagonistic, CTC.ltoreq.120 is additive, CTC.ltoreq.120 is synergistic, and the results are shown in Table 3.
Table 4 pathogenic bacteria screened for capsicum root rot: fusarium solani: indoor virulence assay of (Fusarium solani (Mart.)) App.Et Wollenw
From Table 4, it is clear that when the combination of tetrazolium picolinate and metalaxyl-M is in the range of 50:1-1:50, the pathogenic bacteria selected for root rot of capsicum: fusarium solani: the co-toxicity coefficients of (Fusarium solani (Mart.) App.Et Wollenw) are all more than 120, and the synergistic effect is obvious.
Field efficacy test
In order to better illustrate the effective prevention and treatment effect of the invention, the field efficacy prevention and treatment of solanaceae plants are carried out by using the medicament of the embodiment, 10% of the tetrazolium ammonia ester suspending agent and 20% of the metalaxyl-M aqueous emulsion, and the test results are as follows.
Test five: prevention and treatment effect of tetrazolium picolinate and metalaxyl-M on tomato late blight
The field efficacy test is carried out by setting the total of 12 treatments of the above 9 example medicaments, 2 comparison medicaments and 1 clear water comparison, wherein each treatment is repeated three times, 36 cells are total, and a single cell is set as the sterile detoxified seedling cultivated by 250 seedling raising beds. All treated tomato seedlings were transplanted into pathogen-infested soil-filled plastic pots for manual cultivation for 24 hours. The planting habits of all the communities are the same as the local planting habits, the root irrigation method is used for applying the pesticide, the disease plant rate is counted for 7 days and 14 days after the pesticide is applied for 24 hours, and the control effect is calculated. The survey criteria were: plants have obvious disease symptoms; five-point sampling method is adopted in investigation, 5 points are sampled in each cell, 20 strains are sampled in each point, the number of samples is 100, excel table statistical analysis is adopted in experimental data, and the results are shown in table 5.
TABLE 5 control effects of different treatments on tomato late blight
Note that: the values in the table are the mean of three replicates
The test results in table 5 show that the control effect of the applied medicament on the tomato late blight is obviously higher than that of the comparative medicament, the control effect of the applied medicament on the tomato late blight can reach about 90% after 7 days of administration, the control effect of the applied medicament on the tomato late blight is still kept at a higher level after 14 days of administration, and the control effect of the applied medicament on the tomato late blight is as high as 91.53% in the embodiment 1. The compound bactericide of the tetrazole picoline and the metalaxyl-M has obvious synergistic effect, and the quick acting property and the lasting time are obviously longer than those of a single medicament.
Experiments show that the compound composition obviously reduces the dosage of the medicament, and reduces the production cost, pesticide residue and environmental pollution. In addition, the field observation during the test period shows that each treatment liquid of the agent of the embodiment has no influence on the growth of crops, is safe to surrounding beneficial organisms and has good environmental compatibility.
Test six: prevention and treatment effect of tetrazolium picolinate and metalaxyl-M on brown root rot of tomatoes
The field efficacy test is carried out by setting the total of 12 treatments of the above 9 example medicaments, 2 comparison medicaments and 1 clear water comparison, wherein each treatment is repeated three times, 36 cells are total, and a single cell is set as the sterile detoxified seedling cultivated by 250 seedling raising beds. All treated tomato seedlings were transplanted into pathogen-infested soil-filled plastic pots for manual cultivation for 24 hours. The planting habits of all the communities are the same as the local planting habits, the root irrigation method is used for applying the pesticide, the disease plant rate is counted for 7 days and 14 days after the pesticide is applied for 24 hours, and the control effect is calculated. The survey criteria were: the root of the plant has obvious disease symptoms; five-point sampling method is adopted in investigation, 5 points are sampled in each cell, 20 strains are sampled in each point, the sample number is 100 strains, excel table statistical analysis is adopted in experimental data, and the results are shown in table 6.
TABLE 6 control effects of different treatments on brown root rot of tomato
Note that: the values in the table are the mean of three replicates
From the test results in table 6, the control effect of the applied agent on the brown root rot of the tomatoes is obviously higher than that of the comparative agent, the average control effect of the embodiment after the application of the agent can reach 87.72%, the embodiment 1 reaches 94.14%, and the control effect of the applied agent on the brown root rot of the tomatoes is still kept at a higher level after the application of the agent for 14 days, and the embodiment 1 reaches 92.95%. The compound bactericide of the tetrazole picoline and the metalaxyl-M has obvious synergistic effect, and the quick acting property and the lasting time are obviously longer than those of a single medicament. Experiments show that the compound composition obviously reduces the dosage of the medicament, and reduces the production cost, pesticide residue and environmental pollution. In addition, the field observation during the test period shows that each treatment liquid of the agent of the embodiment has no influence on the growth of crops, is safe to surrounding beneficial organisms and has good environmental compatibility.
Test seven: prevention and treatment effect of tetrazolium picolinate and metalaxyl-M on eggplant root rot
The field efficacy test is carried out by setting the total of 12 treatments of the above 9 example medicaments, 2 comparison medicaments and 1 clear water comparison, wherein each treatment is repeated three times, 36 cells are total, and a single cell is set as the sterile detoxified seedling cultivated by 250 seedling raising beds. All treated eggplant seedlings are transplanted into soil filled plastic pots infected by pathogenic bacteria for artificial cultivation for 24 hours. The planting habits of all the communities are the same as the local planting habits, the root irrigation method is used for applying the pesticide, the disease plant rate is counted for 7 days and 14 days after the pesticide is applied for 24 hours, and the control effect is calculated. The survey criteria were: the root of the plant has obvious disease symptoms; five-point sampling method is adopted in investigation, 5 points are sampled in each cell, 20 strains are sampled in each point, the number of samples is 100, excel table statistical analysis is adopted in experimental data, and the results are shown in table 7.
TABLE 7 prevention and treatment effects of different treatments on eggplant root rot
Note that: the values in the table are the mean of three replicates
The test results in table 7 show that the control effect of the applied medicament on the root rot of the eggplant is obviously higher than that of the comparative medicament, the control effect of the embodiment after the medicament is about 90 percent, the embodiment 1 is up to 95.97 percent, and the control effect of the applied medicament on the root rot of the eggplant is still kept at a higher level after the medicament is applied for 14 days, and the embodiment 1 is up to 93.58 percent. The compound bactericide of the tetrazole picoline and the metalaxyl-M has obvious synergistic effect, and the quick acting property and the lasting time are obviously longer than those of a single medicament. Experiments show that the compound composition obviously reduces the dosage of the medicament, and reduces the production cost, pesticide residue and environmental pollution. In addition, the field observation during the test period shows that each treatment liquid of the agent of the embodiment has no influence on the growth of crops, is safe to surrounding beneficial organisms and has good environmental compatibility.
Test eight: prevention and treatment effect of mixed combination of tetrazolium picolinate and metalaxyl-M on root rot of capsicum
The field efficacy test is carried out by setting the total of 12 treatments of the above 9 example medicaments, 2 comparison medicaments and 1 clear water comparison, wherein each treatment is repeated three times, 36 cells are total, and a single cell is set as the sterile detoxified seedling cultivated by 250 seedling raising beds. All treated pepper seedlings were transplanted into pathogenic bacteria infested soil filled plastic pots for manual cultivation for 24 hours. The planting habits of all the communities are the same as the local planting habits, the root irrigation method is used for applying the pesticide, the disease plant rate is counted for 7 days and 14 days after the pesticide is applied for 24 hours, and the control effect is calculated. The survey criteria were: the root of the plant has obvious disease symptoms; five-point sampling method is adopted in investigation, 5 points are sampled in each cell, 20 strains are sampled in each point, the sample number is 100 strains, excel table statistical analysis is adopted in experimental data, and the results are shown in table 8.
TABLE 8 control effects of different treatments on capsicum root rot
Note that: the values in the table are the mean of three replicates
The test results in table 8 show that the control effect of the applied medicament on the capsicum root rot is obviously higher than that of the compared medicament, the control effect of the applied medicament on the capsicum root rot can reach about 85% after 7 days of administration, the control effect of the applied medicament on the capsicum root rot is still kept at a higher level after 14 days of administration in the embodiment 3, and the control effect of the applied medicament on the capsicum root rot is as high as 86.91%. The compound bactericide of the tetrazole picoline and the metalaxyl-M has obvious synergistic effect, and the quick acting property and the lasting time are obviously longer than those of a single medicament. Experiments show that the compound composition obviously reduces the dosage of the medicament, and reduces the production cost, pesticide residue and environmental pollution. In addition, the field observation during the test period shows that each treatment liquid of the agent of the embodiment has no influence on the growth of crops, is safe to surrounding beneficial organisms and has good environmental compatibility.

Claims (9)

1. The bactericide for the plant growth period is characterized by comprising an active component and an auxiliary material, wherein the active component comprises tetrazole picoline and metalaxyl-M, and the weight ratio of the active component to the metalaxyl-M is 50:1-1:50, preferably 1:10-10:1.
2. The bactericide for plant growth period according to claim 1, characterized in that the addition amount of the active component is 10% to 80% of the total mass of the composition.
3. The plant growing period fungicide according to claim 1 or 2, wherein the auxiliary materials comprise a carrier and an auxiliary agent;
the carrier is selected from at least one of water, solvent or filler;
the auxiliary agent comprises a surfactant or a surfactant and a functional auxiliary agent.
4. A plant growing period fungicide according to claim 3, wherein the surfactant is selected from at least one of an emulsifier, a dispersant, a wetting agent and a penetrant;
the functional auxiliary agent is at least one selected from antifreezing agents, thickening agents, stabilizing agents, disintegrating agents and defoaming agents.
5. A plant growth phase fungicide according to any one of claims 1 to 4, wherein the plant growth phase fungicide is formulated with pesticide formulation processing aid ingredients into any one of the dosage forms suitable for agricultural use.
6. The plant growing period fungicide according to claim 5, wherein the dosage form is selected from at least one of a suspending agent, a microemulsion, an aqueous emulsion, a wettable powder, a water dispersible granule and an emulsifiable concentrate.
7. Use of the plant growth phase fungicide according to any one of claims 1 to 6 for controlling diseases caused by harmful pathogenic fungi of root rot, fusarium, phytophthora.
8. Use according to claim 7, wherein the harmful pathogenic bacteria are selected from at least one of phytophthora parasitica, eggplant root rot pathogen, capsicum root rot pathogen, in particular from at least one of phytophthora parasitica, acanthamoeba, fusarium solani and fusarium solani.
9. Use according to claim 7 or 8, characterized in that the plant is a solanaceous plant selected from capsicum, eggplant or tomato or other crop of peanut, maize.
CN202310583666.3A 2023-05-23 2023-05-23 Bactericide for plant growth period and application thereof Withdrawn CN116602311A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110159110A1 (en) * 2008-03-24 2011-06-30 Nippon Soda Co., Ltd. Plant disease control agent
CN109832289A (en) * 2017-11-29 2019-06-04 佛山市盈辉作物科学有限公司 A kind of composition pesticide containing Picarbutrazox and Metalaxyl-M

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110159110A1 (en) * 2008-03-24 2011-06-30 Nippon Soda Co., Ltd. Plant disease control agent
CN109832289A (en) * 2017-11-29 2019-06-04 佛山市盈辉作物科学有限公司 A kind of composition pesticide containing Picarbutrazox and Metalaxyl-M

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