CN116510799A - Double-layer turntable type pipetting workstation and application method thereof - Google Patents

Abstract

The invention relates to the technical field of pipetting workstations, in particular to a double-layer turntable pipetting workstation and a use method thereof, wherein the double-layer turntable pipetting workstation comprises an upper disc, a lower disc, a driving mechanism and a pipetting module, the upper disc is arranged above the lower disc, the driving mechanism is in power connection with the upper disc and the lower disc, and the driving mechanism can drive the upper disc and the lower disc to rotate; the upper disc is provided with a plurality of consumable boxes for loading suction heads; the lower disc is provided with a plurality of constant temperature modules, a plurality of magnetic force modules for adsorbing magnetic beads, a plurality of dry mixing pore plates and a liquid storage pore plate; the constant temperature module is provided with a constant temperature pore plate, and the magnetic force module is provided with a magnetic attraction pore plate; the pipetting module is movable between an upper tray and a lower tray. The pipetting module only moves in the Z-axis direction, the control difficulty is lower, the required control precision is lower, the fault tolerance is higher, the reliability is higher, and the suction head can not be leaked or dropped due to the deviation in the control movement.

Description

Double-layer turntable type pipetting workstation and application method thereof

Technical Field

The invention relates to the technical field of pipetting workstations, in particular to a double-layer turntable pipetting workstation and a using method thereof.

Background

In the field of molecular diagnosis, complex liquid treatment works such as pipetting, gradient dilution, liquid separation, liquid combination and the like are needed before a sample is put on the machine, such as real-time fluorescence quantitative PCR, NGS gene sequencing and the like.

Thanks to the use of multichannel pipettes, laboratory efficiency today has improved considerably. However, the use of the multichannel pipettor only improves the working efficiency, but does not liberate both hands, and long-time manual pipetting is still a monotonous and heavy work, and may cause repetitive strain injury.

In addition, the increasingly smaller sample adding amount makes the accuracy and the error-free pipetting more difficult, and the manual operation error is uncontrollable, so that the repeatability of the experimental result is poor. Moreover, such experiments are extremely sensitive to environmental pollution, generally require operation on an ultra clean bench, require periodic cleaning, sterilization, etc., and in addition, are prone to aerosol contamination during operation, and are a potential hazard to operators.

The automatic pipetting workstation is a full-automatic operation platform for laboratory liquid treatment operation, can replace manual liquid treatment, reduces the problems of leakage, dislocation, pollution and the like caused by manual operation, can greatly improve the working efficiency, and meets the experiment requirement of high flux.

The existing automatic pipetting workstations generally perform three-dimensional (XYZ) motion control on pipettes, and deep pore plates filled with samples to be processed are arranged in a plane (XY), so that the operation and control are complex, the position accuracy requirement is high, and the running reliability of the instruments cannot be guaranteed. And the instruments generally require users to compile motion time sequences according to actual application scenes, and the complicated operation control mode and man-machine interaction mode can damage the operation experience.

For example, there is a fully automatic pipetting station comprising a frame with a receiving space therein; the moving piece can move in the X-axis and Y-axis directions above the accommodating space; the Z-axis driving modules are arranged on the moving piece; the liquid transferring part is detachably arranged on the Z-axis driving module; the pipetting part comprises a pipetting needle which can move up and down through a screw rod driving motor; the bottom of the pipetting needle is provided with a machine head for adsorbing the pipetting tip; the pipetting head is connected with the machine head; be equipped with a plurality of boards on the board position subassembly for place abandonment groove, consumptive material and reagent pipe.

In the conventional pipetting workstation, the three-dimensional (XYZ) control of a pipetting device has high requirement on position accuracy, the reliability is difficult to ensure, and the pipetting device is easy to leak or drop due to position deviation, so that liquid leakage is caused; meanwhile, as the pipettor needs to be controlled in three dimensions, the working hour is long and the efficiency is low when the operations such as construction and purification of PCR and NGS reaction systems and nucleic acid extraction are carried out.

Disclosure of Invention

The invention provides a double-layer turntable type pipetting workstation and a using method thereof, which aim to solve the problems that a pipetting device in the prior art needs three-dimensional control movement, and has low fault tolerance and poor reliability.

In order to solve the technical problems, the invention adopts the following technical scheme: the double-layer turntable type pipetting workstation comprises an upper disc, a lower disc, a driving mechanism and a pipetting module, wherein the upper disc is arranged above the lower disc, the driving mechanism is in power connection with the upper disc and the lower disc, and the driving mechanism can drive the upper disc and the lower disc to rotate; the upper disc is provided with a plurality of consumable boxes for loading suction heads; the lower disc is provided with a plurality of constant temperature modules, a plurality of magnetic force modules for adsorbing magnetic beads, a plurality of dry mixing pore plates and a liquid storage pore plate; the constant temperature module is provided with a constant temperature pore plate, and the magnetic force module is provided with a magnetic attraction pore plate; the pipetting module is movable between an upper tray and a lower tray.

The upper disc is used for loading a consumable box, the consumable box is internally loaded with a liquid-transferring suction head, the lower disc is used for installing a magnetic module, a constant temperature module, a uniformly-mixed pore plate and a liquid-storing pore plate, and the magnetic module is used for controlling the adsorption and release of magnetic beads in the magnetic pore plate; the isothermal module is used for incubating the nucleic acid fragments in the reagent solution. The pipetting module is connected with a suction head in the consumable box of the upper disc and then moves to the lower disc for pipetting. The upper disc and the lower disc are rotatably arranged, so that the pipetting module does not need to move in the X, Y direction, but the parts on the upper disc or the lower disc correspond to the pipetting module through the rotation of the upper disc or the lower disc, and the pipetting module can operate all the parts on the upper disc and the lower disc only by moving in the Z-axis direction.

Further, the driving mechanism comprises a first driving component, a second driving component and a stand column; the upper disc and the lower disc are all arranged on the upright post in a penetrating way; the first driving component is arranged on the upper disc and can drive the upper disc to rotate around the upright post; the second driving component is arranged on the lower disc and can drive the lower disc to rotate around the upright post; the pipetting module is movably arranged on the upright post and can linearly move along the upright post; the upper plate is provided with a first slot for the pipetting module to pass through.

Further, the pipetting module comprises a driving cylinder and a pipetting device, wherein the driving cylinder is arranged on the upright post, and the pipetting device is arranged on the output end of the driving cylinder.

Further, a second slot for the pipetting module to pass through is formed in the lower plate; and a waste box is arranged below the lower disc.

Further, the magnetic force module comprises a plurality of magnetic plates with magnetism, a driving piece and a connecting component; the magnetic plates are movably arranged on the lower disc in a penetrating way, and gaps which can accommodate the pipe bodies of the magnetic attraction plates are arranged between the adjacent magnetic plates; the driving piece is arranged on the lower disc, and is in power connection with the magnetic plate through the connecting component, and the driving piece drives the magnetic plate to move up and down through the connecting component.

Further, the connecting assembly comprises a first connecting rod, a second connecting rod and a supporting plate; the magnetic plate is arranged at the top of the supporting plate, and the first connecting rod is connected with the output end of the driving piece; one end of the second connecting rod is hinged with the first connecting rod, and the other end of the second connecting rod is hinged with the supporting plate.

Further, the constant temperature module comprises a heating sheet, a refrigerating sheet, a temperature control sensor and a heat dissipation assembly; the refrigerating sheet is arranged at the top end of the heating sheet; the heating sheet is arranged at the top end of the heat dissipation component; the temperature control sensor is arranged on the heating sheet; the constant temperature orifice plate is arranged at the top of the refrigerating sheet and the heating sheet.

Further, the device also comprises a code scanner which scans bar codes on the mixing pore plate, the magnetic attraction pore plate, the liquid storage pore plate and the constant temperature pore plate

On the other hand, the invention provides a use method of the double-layer turntable type pipetting workstation, which comprises the following steps:

s1, rotating an upper disc to a position where any consumable box is opposite to a pipetting module, then moving the pipetting module downwards until the pipetting module is connected with a suction head in the consumable box, and then moving upwards again by the pipetting module provided with the suction head to separate the suction head from the consumable box;

s2, the lower disc rotates to a position where the liquid storage pore plate is opposite to the lower side of the liquid transfer module, then the liquid transfer module moves downwards to a position where the suction head is inserted into reagent liquid in the liquid storage pore plate, the liquid transfer module sucks a set amount of reagent liquid, and after the suction is finished, the liquid transfer module moves upwards to a position where the suction head is separated;

s3, rotating the lower disc to a position where the mixing pore plate is opposite to the liquid transferring module, then moving the liquid transferring module downwards to a position where the suction head is inserted into the mixing pore plate, repeatedly discharging and sucking the sucked reagent liquid into the mixing pore plate by the liquid transferring module through the suction head, uniformly mixing the reagent liquid, sucking the reagent liquid into the liquid transferring module again after the number of times of discharging and sucking is set, and simultaneously moving the liquid transferring module upwards to a position where the reagent liquid is separated from the mixing pore plate, so as to finish the mixing operation;

s4, the lower disc rotates to a position where the temperature control module is opposite to the pipetting module, then the pipetting module moves downwards to a position where the suction head is inserted into the thermostatic orifice plate, the pipetting module injects reagent liquid into the thermostatic orifice plate on the thermostatic module, after the thermostatic module keeps the reagent liquid in the thermostatic orifice plate at a constant set temperature and for a set time, the pipetting module sucks the reagent liquid in the thermostatic orifice plate into the pipetting module again, and meanwhile the pipetting module moves upwards to a position where the pipetting module is separated from the thermostatic orifice plate, so that the thermostatic incubation operation is completed;

s5, the lower disc rotates to a position where the magnetic force module is opposite to the liquid suction module, then the liquid suction module moves downwards to a position where the suction head is inserted into the magnetic suction orifice plate, the liquid suction module injects liquid into the magnetic suction orifice plate, the liquid suction module moves upwards to a position where the liquid suction module is separated from the magnetic suction orifice plate, then the magnetic force module performs magnetic suction purification on reagent liquid in the magnetic suction orifice plate for a set time, then the liquid suction module moves downwards to the suction head again to be inserted into the magnetic suction orifice plate, the reagent liquid after magnetic suction purification is sucked into the liquid suction module, and the liquid suction module moves upwards to a position where the reagent liquid is separated from the magnetic suction orifice plate again, so that the magnetic suction purification operation is completed;

s6, the lower disc rotates to a position where the liquid storage pore plate is opposite to the liquid storage pore plate, the liquid storage module moves downwards to a position where the suction head is inserted into the liquid storage pore plate, the liquid storage module injects the reagent liquid subjected to magnetic purification into the liquid storage pore plate, and then the liquid storage module moves upwards to a position where the reagent liquid is separated from the liquid storage pore plate, so that a purified product is obtained.

Compared with the prior art, the invention has the beneficial effects that:

1. compared with the existing X, Y, Z three-axis moving pipetting module, the pipetting module has the advantages that the pipetting module only needs to move in the Z-axis direction, the control difficulty is lower, the required control precision is lower, the fault tolerance is higher, the reliability is higher, and the suction head cannot be leaked or dropped due to deviation in the control movement;

2. in the magnetic module, the lifting and the descending of the magnetic plate are controlled by the driving piece, the pore plate is arranged above the magnetic plate, and when the magnetic plate is lifted, the magnetic plate is close to the magnetic pore plate, so that magnetic beads in the magnetic pore plate can be attracted and gathered, the magnetic plate is far away from the magnetic pore plate, the magnetic beads in the magnetic pore plate are released, and the effect of performing magnetic purification operation without moving the magnetic pore plate is achieved;

3. by arranging the code scanner, a user can set bar code or two-dimensional code information corresponding to the plate position when setting a motion flow, and the code scanner is used for identifying the bar code or the two-dimensional code of the plate position loaded with the reagent liquid, and comparing and judging whether the reagent liquid is loaded correctly;

4. the double-layer turntable type pipetting workstation can rapidly perform operations such as construction and purification of a reaction system of the NGS, nucleic acid extraction and the like, and compared with the existing pipetting workstation, the pipetting workstation provided by the invention simplifies the control dimension of a pipetting module to only one residual Z axis through the layout of the upper and lower double-layer turntables, so that the operations such as construction and purification of the reaction system of the NGS, nucleic acid extraction and the like are simplified, and the operation efficiency of the application is improved.

Drawings

FIG. 1 is a schematic overall construction of an embodiment of a dual-layer carousel pipetting station of the invention;

FIG. 2 is a schematic view showing the internal structure of an embodiment of a double-deck turntable-type pipetting workstation of the invention;

FIG. 3 is a schematic view of the internal structure of another perspective of an embodiment of a dual-layer carousel pipetting station of the invention;

FIG. 4 is a schematic view showing the structure of the upper tray and the lower tray of the double-layer turntable type pipetting workstation in accordance with the embodiment of the invention;

FIG. 5 is a schematic view of the structure of the bottom wall of an embodiment of a dual-layer carousel pipetting station of the invention;

FIG. 6 is a schematic diagram of the magnetic module in an embodiment of a dual-layer carousel pipetting station of the invention;

FIG. 7 is a schematic diagram of a magnetic module from another perspective in an embodiment of a dual-layer carousel pipetting station in accordance with the invention;

FIG. 8 is a schematic view of the structure of a constant temperature module in an embodiment of a dual-layer carousel pipetting station of the invention;

fig. 9 is a schematic view of a thermostatic module from another perspective in an embodiment of a dual-layer turntable-type pipetting station of the invention.

In the accompanying drawings: 1. a top plate; 2. a lower plate; 3. a driving mechanism; 31. a first drive assembly; 32. a second drive assembly; 33. a column; 4. a pipetting module; 41. a drive cylinder; 42. a pipette; 5. a consumable box; 6. a constant temperature module; 61. a thermostatic orifice plate; 62. heating sheet; 63. a cooling sheet; 64. a temperature control sensor; 65. a heat dissipation assembly; 651. a heat radiation fin; 652. a heat radiation fan; 653. a case body; 7. a magnetic force module; 71. a magnetic attraction pore plate; 72. a magnetic plate; 73. a driving member; 74. a connection assembly; 741. a first link; 742. a second link; 743. a support plate; 75. a fixing plate; 76. a slide rail; 77. a slide block; 78. a connecting plate; 8. a liquid storage pore plate; 9. mixing well the pore plate; 10. a controller; 11. a substrate; 12. a first slot; 13. a second slot; 14. a reject box; 15. a housing; 16. a taking and placing port; 17. a cabin door; 18. a code scanner; 19. and a protective cover.

Detailed Description

The drawings are for illustrative purposes only and are not to be construed as limiting the present patent; for the purpose of better illustrating the embodiments, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the actual product dimensions; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationship depicted in the drawings is for illustrative purposes only and is not to be construed as limiting the present patent.

The same or similar reference numbers in the drawings of embodiments of the invention correspond to the same or similar components; in the description of the present invention, it should be understood that, if there are orientations or positional relationships indicated by terms "upper", "lower", "left", "right", "long", "short", etc., based on the orientations or positional relationships shown in the drawings, this is merely for convenience in describing the present invention and simplifying the description, and is not an indication or suggestion that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so that the terms describing the positional relationships in the drawings are merely for exemplary illustration and are not to be construed as limitations of the present patent, and that it is possible for those of ordinary skill in the art to understand the specific meaning of the terms described above according to specific circumstances.

The technical scheme of the invention is further specifically described below through specific embodiments and with reference to the accompanying drawings.

Example 1

Referring to fig. 1 to 9, an embodiment 1 of a double-layer turntable type pipetting workstation according to the invention comprises an upper plate 1, a lower plate 2, a driving mechanism 3 and a pipetting module 4, wherein the upper plate 1 is arranged above the lower plate 2, the driving mechanism 3 is in power connection with the upper plate 1 and the lower plate 2, and the driving mechanism 3 can drive the upper plate 1 and the lower plate 2 to rotate; the upper disc 1 is provided with a plurality of consumable boxes 5 for loading suction heads; the lower disc 2 is provided with a plurality of constant temperature modules 6, a plurality of magnetic force modules 7 for adsorbing magnetic beads, a plurality of uniformly mixed pore plates 9 and a liquid storage pore plate 8; a constant temperature pore plate 61 is arranged on the constant temperature module 6, and a magnetic pore plate 71 loaded with magnetic beads is arranged on the magnetic force module 7; the pipetting module 4 is movable between the upper tray 1 and the lower tray 2.

The upper disc 1 is used for loading a consumable box 5, a liquid-transferring suction head is loaded in the consumable box 5, the lower disc 2 is used for installing a magnetic force module 7, a constant temperature module 6, a mixing pore plate 9 and a liquid-storing pore plate 8, and the magnetic force module 7 is used for controlling the adsorption and release of magnetic beads in the magnetic attraction pore plate 71; the thermostating module 6 is used to incubate nucleic acid fragments within the reagent solution. The pipetting module 4 connects the pipette tips to the consumable cassette 5 of the upper plate 1 and then moves to the lower plate 2 for pipetting. The upper disc 1 and the lower disc 2 are rotatably arranged, so that the pipetting module 4 does not need to move in the X, Y direction, but the components on the upper disc 1 or the lower disc 2 correspond to the pipetting module 4 through the rotation of the upper disc 1 or the lower disc 2, and the pipetting module 4 can operate all the components on the upper disc 1 and the lower disc 2 only by moving in the Z-axis direction.

In this embodiment, the driving mechanism 3 includes a first driving component 31, a second driving component 32, and a post 33; the upper disc 1 and the lower disc 2 are all arranged on the upright post 33 in a penetrating way; the first driving component 31 is arranged on the upper disc 1, and the first driving component 31 can drive the upper disc 1 to rotate around the upright post 33; the second driving component 32 is arranged on the lower disc 2, and the second driving component 32 can drive the lower disc 2 to rotate around the upright post 33; the pipetting module 4 is movably arranged on the upright post 33, and the pipetting module 4 can move linearly along the upright post 33; the upper plate 1 is provided with a first slot 12 for the pipetting module 4 to pass through.

Specifically, the first driving component 31 and the second driving component 32 are hollow rotating platforms, the first driving component 31 is disposed at the top of the upper disc 1, and the output bearing of the first driving component 31 is coaxially disposed with the upper disc 1, and the output bearing of the first driving component 31 is mounted at the top of the upper disc 1 through bolts. The second driving component 32 is arranged on the top of the lower disc 2, and an output bearing of the second driving component 32 is coaxially arranged with the lower disc 2, and the output bearing of the second driving component 32 is arranged on the top of the lower disc 2 through bolts. The pillar 33 has a cylindrical shape, and the pillar 33 is fixed to the lowermost base plate 11 by bolts. The upright post 33 is coaxially penetrated through the upper disc 1 and the lower disc 2, and the upright post 33 is also penetrated through the output bearing of the first driving component 31 on the upper disc 1 and the output bearing of the second driving component 32 on the lower disc 2, and the upright post 33 is fixedly connected with the inner ring of the output bearing of the first driving component 31 on the upper disc 1 and the inner ring of the output bearing of the driving component 32 on the lower disc 2, so that the first driving component 31 on the upper disc 1 can drive the upper disc 1 to rotate by taking the upright post 33 as an axis, and the second driving component 32 on the lower disc 2 drives the lower disc 2 to rotate by taking the upright post 33 as an axis. The upper plate 1 is provided with a square first slot 12, so that the pipetting module 4 can pass through the upper plate 1 to a position close to the lower side of the upper plate 1 through the first slot 12 in order to interact with the components on the lower plate 2.

In this embodiment, the pipetting module 4 includes a driving cylinder 41 and a pipetting device 42, the driving cylinder 41 is disposed on the column 33, and the pipetting device 42 is disposed on an output end of the driving cylinder 41.

Specifically, the periphery of the upright post 33 is covered with two protecting covers 19, one protecting cover 19 is arranged above the upper disc 1, the other protecting cover 19 is arranged above the lower disc 2, and a gap is reserved between the two protecting covers 19, so that the upper disc 1 can conveniently rotate. The driving cylinder 41 is a rodless electric cylinder, the driving cylinder 41 is mounted on the protective cover 19 above the upper plate 1 through bolts, and the travel direction of the driving cylinder 41 is the same as the axial direction of the upright post 33. The pipette 42 is a 96-channel pipette, and the pipette 42 is mounted and fixed on the slider 77 of the drive cylinder 41 by bolts. The driving cylinder 41 drives the pipette 42 to move up and down so that the pipette 42 can interact with the respective components on the upper tray 1 or the lower tray 2.

In this embodiment, the bottom wall 2 is provided with a second slot 13 for the pipetting module 4 to pass through; a reject box 14 is arranged below the lower tray 2.

Specifically, the waste box 14 is fixed on the base plate 11 by bolts, the waste box 14 is located right below the vertical direction of the pipetter 42, the waste box 14 is square, the top of the waste box 14 is open, and the area of the opening at the top of the waste box 14 is larger than the projection area of the second slot 13 on the base plate 11, so that the suction head or the waste liquid can be conveniently discarded into the waste box 14.

In this embodiment, the magnetic module 7 includes a plurality of magnetic plates 72 with magnetism, a driving member 73, and a connecting assembly 74; the magnetic plates 72 movably penetrate through the lower disc 2, and gaps which can accommodate the tube bodies of the magnetic attraction plate 71 are arranged between the adjacent magnetic plates 72; the driving piece 73 is arranged on the lower disc 2, the driving piece 73 is in power connection with the magnetic plate 72 through the connecting component 74, and the driving piece 73 drives the magnetic plate 72 to move up and down through the connecting component 74. The connecting assembly 74 includes a first link 741, a second link 742, and a support plate 743; the magnetic plate 72 is arranged at the top of the supporting plate 743, and the first connecting rod 741 is connected with the output end of the driving member 73; one end of the second link 742 is hinged to the first link 741, and the other end is hinged to the support plate 743.

Specifically, the magnetic plates 72 are square plate-shaped made of permanent magnets, and each magnetic module 7 includes six magnetic plates 72, and the six magnetic plates 72 are inserted in parallel and vertically on the support plate 743. The lower plate 2 is provided with a plurality of square notches, and the support plate 743 can be inserted into the square notches and move up and down therein. The driving member 73 is a motor, and the driving member 73 is fixed by bolts at a position near the notch on the bottom surface of the lower plate 2. The lower plate 2 is provided with a magnetic attraction plate 71 above the notch. The driving motor drives the supporting plate 743 to pass through the notch through the connecting assembly 74 and penetrate the lower disc 2 to move up and down. The driving piece 73 drives the magnetic plate 72 to move upwards to be close to the magnetic attraction plate 71, so that magnetic beads in the magnetic attraction plate 71 can be attracted and gathered at the bottom of the magnetic attraction plate 71, and the effect of performing magnetic liquid separation under the condition that the magnetic attraction plate 71 is not moved is achieved.

The driving piece 73 is fixed on a fixed plate 75 through a bolt, the top of the fixed plate 75 is connected with the bottom of the lower disc 2 through a bolt, two sliding rails 76 are arranged on the side wall of the fixed plate 75 far away from the driving piece 73, the travel direction of the sliding rails 76 is vertical, a sliding block 77 is respectively and slidably embedded on the two sliding rails 76, two connecting plates 78 are arranged on the sliding block 77, one end of each connecting plate 78 is welded with the sliding block 77, and the other end is welded with a supporting plate 743. The output end of the driving member 73 passes through the fixing plate 75 at a position between the two slide rails 76 and is in power connection with the support plate 743 through the connection assembly 74. By arranging the slide rail 76 and the slide block 77, the support plate 743 moves up and down more stably, and the support plate 743 is ensured to move along a straight line. The first link 741 is fixed to the output end of the driving member 73 through a bolt, so that the driving member 73 can drive the second link 742 to drive the support plate 743 to move up and down along the sliding rail 76 through the first link 741.

In this embodiment, the constant temperature module 6 includes a heating plate 62, a cooling plate 63, a temperature control sensor 64, and a heat dissipation assembly 65; the refrigerating plate 63 is arranged at the top end of the heating plate 62; the heating plate 62 is arranged at the top end of the heat dissipation assembly 65; the temperature control sensor 64 is arranged on the refrigerating plate 63 and the heating plate 62; the thermostatic orifice plate 61 is arranged on top of the cooling plate 63 and the heating plate 62.

Specifically, the cooling plate 63 is a TEC semiconductor plate, the heating plate 62 is a mica plate, and the temperature control sensor 64 is used for sensing the temperatures of the cooling plate 63 and the heating plate 62, and simultaneously controlling the temperature of the cooling plate 63 or the heating plate 62 so that the thermostatic orifice plate 61 can maintain a set temperature. The heat dissipation component 65 is used for dissipating heat of the mica sheet, and preventing the temperature from being too high.

In this embodiment, the heat dissipation assembly 65 includes a heat dissipation fin 651 and a heat dissipation fan 652; the heat dissipation fins 651 are arranged on the air outlet of the heat dissipation fan 652; the heating plate 62 is provided on top of the heat dissipation fins 651.

Specifically, the cooling fan 652 and the cooling fins 651 are mounted in a box 653, and the box 653 is screwed to the bottom surface of the lower plate 2, and the cooling fins 651 are disposed through the lower plate 2, so that the height of the entire constant temperature module 6 can be reduced. The heat radiation fins 651 can increase the heat transfer efficiency of the heating plate 62.

In this embodiment, the device further comprises a housing 15, the upper disc 1, the lower disc 2 and the upright post 33 are all disposed in the housing 15, a picking and placing port 16 for picking and placing the hole plate is disposed on the housing 15, and a cabin door 17 is movably disposed at the position of the picking and placing port 16 on the housing 15.

Specifically, the casing 15 is provided with a controller 10 capable of being controlled by a touch screen, and the controller 10 is electrically connected with the pipetting module 4, the magnetic attraction module and the constant temperature module 6 to control the pipetting module 4, the magnetic attraction module and the constant temperature module 6. In this embodiment, the controller 10 is a PLC. The controller 10 is mounted on the housing 15 and located at the top of the door 17, the controller 10 is electrically connected with the door 17, and the controller 10 can control the door 17 to open and close.

Example 2

Referring to fig. 3, in order to provide an embodiment 2 of a double-layer turntable type pipetting workstation according to the present invention, the difference between the embodiment and the embodiment 1 is that the double-layer turntable type pipetting workstation further includes a code scanner 18, and the code scanner 18 scans bar codes on the mixing well plate 9, the magnetic attraction well plate 71, the liquid storage well plate 8 and the constant temperature well plate 61. The code scanner 18 is arranged above the lower disc 2, the code scanner 18 is electrically connected with the controller 10, the code scanner 18 scans bar codes on the uniformly mixed pore plate 9, the magnetic attraction pore plate 71, the liquid storage pore plate 8 and the constant temperature pore plate 61, and scanned bar code information is sent to the controller 10 so as to verify whether the positions of all pore plates are correct.

Example 3

The application method of the double-layer turntable type pipetting workstation in the embodiment 1 of the invention comprises the following steps:

s1, rotating an upper disc 1 to a position where any consumable box 5 is opposite to a pipette 42, driving a cylinder 41 to drive the pipette 42 to move downwards until the pipette 42 is connected with a suction head in the consumable box 5, and then upwards moving the pipette 42 provided with the suction head again to separate the suction head from the consumable box 5;

s2, the lower disc 2 rotates to a position where the liquid storage pore plate 8 is opposite to the lower side of the liquid transfer device 42, then the liquid transfer device 42 moves downwards to a position where the suction head is inserted into reagent liquid of the liquid storage pore plate 8, the liquid transfer device 42 sucks a set amount of reagent liquid, and after the suction is finished, the liquid transfer device 42 moves upwards to a position where the suction head is separated;

s3, the lower disc 2 rotates to a position where the mixing hole plate 9 is opposite to the liquid shifter 42, then the liquid shifter 42 moves downwards to a position where the suction head is inserted into the mixing hole plate 9, the liquid shifter 42 repeatedly spits and sucks the sucked reagent liquid into the mixing hole plate 9 through the suction head, the reagent liquid is uniformly mixed, after the number of spit-suction times of a set quantity is completed, the liquid shifter module 4 sucks the reagent liquid again, and meanwhile, the liquid shifter 42 moves upwards to a position where the suction head is separated from the mixing hole plate 9, so that the mixing operation is completed;

s4, the lower disc 2 rotates to a position where the constant temperature module 6 is opposite to the pipetting module 4, then the pipetting device 42 moves downwards to a position where the pipette tip is inserted into the constant temperature orifice plate 61, the pipetting device 42 injects reagent liquid into the constant temperature orifice plate 61 on the constant temperature module 6, after the constant temperature module 6 keeps the reagent liquid in the constant temperature orifice plate 61 at a constant set temperature and for a set time, the pipetting device 42 sucks the reagent liquid in the constant temperature orifice plate 61 again, and meanwhile the pipetting device 42 moves upwards to a position where the pipette tip is separated from the constant temperature orifice plate 61, so that constant temperature incubation operation is completed;

s5, rotating the lower disc 2 to a position where the magnetic module 7 is opposite to the liquid suction plate 71, then moving the liquid suction plate 42 downwards until the liquid suction head is inserted into the liquid suction plate 71, injecting liquid into the liquid suction plate 71 by the liquid suction plate 42, moving the liquid suction module 4 upwards again to a position where the liquid suction head is separated from the liquid suction plate 71, then driving the supporting plate 743 to move upwards by the driving piece 73 so that the magnetic plate 72 is close to the liquid suction plate 71, sucking and gathering magnetic beads in the liquid suction plate 71 at the bottom by the magnetic plate 72, performing magnetic suction purification on reagent liquid in the liquid suction plate 71 for a set time, then moving the liquid suction plate 42 downwards again until the liquid suction head is inserted into the liquid suction plate 71, sucking the reagent liquid after magnetic suction purification into the liquid suction plate, moving the liquid suction plate 42 upwards again until the liquid suction head is separated from the liquid suction plate 71, and simultaneously driving the supporting plate 743 downwards to the magnetic plate 72 to be far away from the liquid suction plate 71 by the driving piece 73 so as to finish the magnetic suction purification operation;

s6, rotating the lower disc 2 to a position where the liquid storage pore plate 8 is opposite to the liquid storage pore plate 42, moving the liquid storage pore plate 42 downwards until the suction head is inserted into the liquid storage pore plate 8, injecting the reagent liquid subjected to magnetic purification into the liquid storage pore plate 8 by the liquid storage pore plate 42, and then moving the liquid transfer module 4 upwards until the suction head is separated from the liquid storage pore plate 8, so as to obtain a purified product; the lower tray 2 is rotated again to a position where the second slot 13 is opposite to the pipette 42, the pipette 42 moves through the second slot 13 to a position close to the reject box 14, the pipette 42 discards the suction head in the reject box 14, and rises again to the upper tray 1 to wait for the next operation.

It is to be understood that the above examples of the present invention are provided by way of illustration only and not by way of limitation of the embodiments of the present invention. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.

Claims (10)

1. The double-layer turntable type pipetting workstation is characterized by comprising an upper disc (1), a lower disc (2), a driving mechanism (3) and a pipetting module (4), wherein the upper disc (1) is arranged above the lower disc (2), the driving mechanism (3) is in power connection with the upper disc (1) and the lower disc (2), and the driving mechanism (3) can drive the upper disc (1) and the lower disc (2) to rotate; the upper disc (1) is provided with a plurality of consumable boxes (5) for loading suction heads; a plurality of constant temperature modules (6), a plurality of magnetic force modules (7) for adsorbing magnetic beads, a plurality of mixing pore plates (9) and a liquid storage pore plate (8) are arranged on the lower disc (2); a constant temperature pore plate (61) is arranged on the constant temperature module (6), and a magnetic pore plate (71) loaded with magnetic beads is arranged on the magnetic force module (7); the pipetting module (4) can move between the upper disc (1) and the lower disc (2).

2. The double-deck carousel pipetting workstation according to claim 1, wherein the drive mechanism (3) comprises a first drive assembly (31), a second drive assembly (32) and a column (33); the upper disc (1) and the lower disc (2) are all arranged on the upright post (33) in a penetrating way; the first driving component (31) is arranged on the upper disc (1), and the first driving component (31) can drive the upper disc (1) to rotate around the upright post (33); the second driving assembly (32) is arranged on the lower disc (2), and the second driving assembly (32) can drive the lower disc (2) to rotate around the upright post (33); the pipetting module (4) is movably arranged on the upright post (33), and the pipetting module (4) can move linearly along the upright post (33); the upper disc (1) is provided with a first slot (12) for the pipetting module (4) to pass through.

3. The double-deck carousel formula pipetting workstation according to claim 2, wherein the pipetting module (4) comprises a drive cylinder (41) and a pipetting device (42), the drive cylinder (41) is arranged on the column (33), and the pipetting device (42) is arranged on the output end of the drive cylinder (41).

4. The double-layer turntable-type pipetting workstation according to claim 2, wherein the bottom wall (2) is provided with a second slot (13) through which the pipetting module (4) passes; and a waste box (14) is arranged below the lower disc (2).

5. The double-deck carousel pipetting workstation according to claim 1, wherein the magnetic module (7) comprises a plurality of magnetic plates (72) having magnetic properties, a driving member (73) and a connection assembly (74); the magnetic plates (72) are movably arranged on the lower disc (2) in a penetrating way, and gaps which can accommodate the tube bodies of the magnetic attraction plate (71) are arranged between the adjacent magnetic plates (72); the driving piece (73) is arranged on the lower disc (2), the driving piece (73) is in power connection with the magnetic plate (72) through the connecting component (74), and the driving piece (73) drives the magnetic plate (72) to move up and down through the connecting component (74).

6. The double-deck carousel-type pipetting station of claim 4, wherein the connection assembly (74) comprises a first link (741), a second link (742) and a support plate (743); the magnetic plate (72) is arranged at the top of the supporting plate (743), and the first connecting rod (741) is connected with the output end of the driving piece (73); one end of the second connecting rod (742) is hinged with the first connecting rod (741), and the other end is hinged with the supporting plate (743).

7. The double-layer carousel pipetting workstation as recited in claim 1, wherein the constant temperature module (6) comprises a heating plate (62), a cooling plate (63), a temperature control sensor (64) and a heat sink assembly (65); the refrigerating sheet (63) is arranged at the top end of the heating sheet (62); the heating sheet (62) is arranged at the top end of the heat radiating component (65); the temperature control sensor (64) is arranged on the refrigerating sheet (63) and the heating sheet (62); the constant temperature pore plate (61) is arranged at the tops of the refrigerating sheet (63) and the heating sheet (62).

8. The double-deck carousel pipetting workstation of claim 6, wherein the heat sink assembly (65) comprises heat sink fins (651) and heat sink fans (652); the radiating fins (651) are arranged on an air outlet of the radiating fan (652); the heating plate (62) is arranged on the top of the radiating fin (651).

9. The double-layer turntable type pipetting workstation according to claim 1, further comprising a code scanner (18), wherein the code scanner (18) scans bar codes on the mixing well plate (9), the magnetic attraction well plate (71), the liquid storage well plate (8) and the constant temperature well plate (61).

10. A method of using a double layer carousel pipetting station as recited in any one of claims 1-9 comprising the steps of:

s1, rotating an upper disc (1) to a position where any consumable box (5) is opposite to a pipetting module (4), then moving the pipetting module (4) downwards until the pipetting module (4) is connected with a suction head in the consumable box (5), and then moving upwards again by the pipetting module (4) provided with the suction head to separate the suction head from the consumable box (5);

s2, the lower disc (2) rotates to a position where the liquid storage pore plate (8) is right opposite to the lower part of the liquid transfer module (4), then the liquid transfer module (4) moves downwards to a position where the suction head is inserted into reagent liquid in the liquid storage pore plate (8), the liquid transfer module (4) sucks a set amount of reagent liquid, and after the suction is finished, the liquid transfer module (4) moves upwards to a position where the suction head is separated from the reagent liquid;

s3, rotating the lower disc (2) to a position where the mixing pore plate (9) is opposite to the liquid transferring module (4), then downwards moving the liquid transferring module (4) to a position where the suction head is inserted into the mixing pore plate (9), repeatedly spitting-sucking the sucked reagent liquid into the mixing pore plate (9) through the suction head by the liquid transferring module (4), uniformly mixing the reagent liquid, and after the spitting-sucking times of a set quantity are completed, sucking the reagent liquid into the liquid transferring module (4) again, and simultaneously upwards moving the liquid transferring module (4) to a position where the reagent liquid is separated from the mixing pore plate (9), so as to complete the mixing operation;

s4, rotating the lower disc (2) to a position where the constant temperature module (6) is opposite to the pipetting module (4), then moving the pipetting module (4) downwards until the pipette tip is inserted into the constant temperature orifice plate (61), injecting reagent liquid into the constant temperature orifice plate (61) on the constant temperature module (6) by the pipetting module (4), keeping the reagent liquid in the constant temperature orifice plate (61) at a constant set temperature by the constant temperature module (6) and keeping the set time, sucking the reagent liquid in the constant temperature orifice plate (61) into the pipetting module (4) again, and simultaneously moving the pipetting module (4) upwards to a position separated from the constant temperature orifice plate (61) to finish constant temperature incubation operation;

s5, rotating the lower disc (2) to a position where the magnetic force module (7) is opposite to the liquid-transferring module (4), then moving the liquid-transferring module (4) downwards to a position where the suction head is inserted into the magnetic-suction hole plate (71), injecting liquid into the magnetic-suction hole plate (71) by the liquid-transferring module (4), then moving the liquid-transferring module (4) upwards to a position where the liquid-transferring module is separated from the magnetic-suction hole plate (71), then performing magnetic suction purification on reagent liquid in the magnetic-suction hole plate (71) for a set time by the magnetic force module (7), then moving the liquid-transferring module (4) downwards to the suction head to be inserted into the magnetic-suction hole plate (71), sucking the reagent liquid after the magnetic suction purification into the liquid-transferring module (4) upwards to the position where the magnetic-suction hole plate (71) is separated, and completing the magnetic suction purification operation;

s6, the lower disc (2) rotates to the position where the liquid storage pore plate (8) is opposite to the liquid storage pore plate (4), the liquid storage module (4) moves down to the position where the suction head is inserted into the liquid storage pore plate (8), the liquid storage module (4) injects the reagent liquid after magnetic purification into the liquid storage pore plate (8), and then the liquid storage module (4) moves up to the position where the reagent liquid is separated from the liquid storage pore plate (8), so that a purified product is obtained.