CN116463197B - Carbon fixation photobioreactor and application thereof - Google Patents
Carbon fixation photobioreactor and application thereof Download PDFInfo
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- CN116463197B CN116463197B CN202310722742.4A CN202310722742A CN116463197B CN 116463197 B CN116463197 B CN 116463197B CN 202310722742 A CN202310722742 A CN 202310722742A CN 116463197 B CN116463197 B CN 116463197B
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 13
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 13
- 238000005507 spraying Methods 0.000 claims abstract description 41
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000006243 chemical reaction Methods 0.000 claims abstract description 20
- 238000009423 ventilation Methods 0.000 claims abstract description 20
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 13
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 13
- 239000007921 spray Substances 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 61
- 239000007789 gas Substances 0.000 claims description 15
- 238000005286 illumination Methods 0.000 claims description 6
- 230000029553 photosynthesis Effects 0.000 claims description 5
- 238000010672 photosynthesis Methods 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 4
- 230000001360 synchronised effect Effects 0.000 claims description 4
- 230000000630 rising effect Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 1
- 239000011259 mixed solution Substances 0.000 abstract description 6
- 230000000243 photosynthetic effect Effects 0.000 abstract description 4
- 241000195493 Cryptophyta Species 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 238000005273 aeration Methods 0.000 description 2
- 238000005276 aerator Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003225 biodiesel Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
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Abstract
The invention discloses a carbon-fixing photobioreactor and application thereof, and relates to the technical field of photobioreactors, the carbon-fixing photobioreactor comprises a reaction tank, a light lamp plate, a spraying mechanism and a ventilation mechanism, wherein the reaction tank comprises a base, the surface of the base is fixedly connected with a transparent culture bin, the light lamp plate is arranged on the outer surface of the culture bin, light of the light lamp plate can irradiate the interior of the culture bin, and a valve mechanism, a power mechanism and a pipeline are arranged in the reaction tank; this solid carbon photobioreactor and application thereof through setting up light lamp board, spraying mechanism, ventilation mechanism, power unit and valve mechanism mutually support for the mixed solution that contains little algae and culture medium accessible spraying mechanism sprays downwards from the top in the cultivation storehouse, fully mixes with the gas of letting in, improves the efficiency of absorbing carbon dioxide wherein through the photosynthetic reaction, thereby reaches the effect that improves solid carbon efficiency.
Description
Technical Field
The invention relates to the technical field of photobioreactors, in particular to a carbon-fixing photobioreactor and application thereof.
Background
A photobioreactor refers to a type of device that can be used for the cultivation of photosynthetic microorganisms and their photosynthetic tissues or cells. The reactor has a similar structure as a general bioreactor, and certain illumination, temperature, nutrient substances and the like are required to culture microorganisms and regulate and control the environment of the system under general conditions. The microalgae is cultivated by utilizing a photobioreactor, and then carbon dioxide is absorbed by utilizing photosynthesis of the microalgae to be converted into biomass, and oxygen is released, so that the carbon fixing effect can be achieved. The microalgae can absorb carbon dioxide through photosynthesis to be converted into biomass and release oxygen, and the microalgae biomass contains substances such as fatty acid, protein, vitamin and the like and can be used for producing high-added-value products such as animal feed, food additives, biodiesel and the like.
Chinese patent publication No. CN102676371B relates to a photobioreactor and its application. The photo-bioreactor comprises a photo-bioreactor main body (5), an air supply device (20), a solution parameter monitoring system (30) and an illumination device (14), and further comprises an aeration system (40), wherein the aeration system (40) comprises a booster pump (1) and an aerator (3), and the aerator (3) is located at the bottom of the photo-bioreactor main body (5) and is connected with the booster pump (1) arranged outside the photo-bioreactor main body (5). The carbon dioxide removal efficiency of the photobioreactor is high, the accumulation of dissolved oxygen of algae liquid in the photobioreactor is small, and algae bodies are not easy to precipitate.
However, when microalgae are cultivated for carbon fixation, the microalgae are inconvenient to continuously introduce and replace, so that the microalgae are continuously cultivated, the carbon fixation efficiency is ensured, and meanwhile, the microalgae cultivation efficiency is improved.
Disclosure of Invention
The invention aims to provide a carbon-fixing photobioreactor and application thereof, so as to solve the defects in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions: the utility model provides a solid carbon photobioreactor and application thereof, includes retort, light lamp plate, spraying mechanism, ventilation mechanism, the retort includes the base, the surface fixedly connected with of base is transparent cultivates the storehouse, the light lamp plate sets up the surface at cultivates the storehouse, the light of light lamp plate can shine inside cultivateing the storehouse, the inside of retort is provided with valve mechanism, power mechanism, pipeline, valve mechanism has a plurality of openings, the pipeline has a plurality of, valve mechanism passes through the opening and is connected with the pipeline to communicate outside with the retort through the pipeline, power mechanism is used for driving the mixed solution and passes through valve mechanism, and adjusts valve mechanism control opening switch for the mixed solution is from outside to be in cultivateing the storehouse or in cultivateing the storehouse circulation;
the spraying mechanism is communicated with the valve mechanism through a port, and the mixed liquid can enter the spraying mechanism through the port and then is sprayed out from the spraying mechanism after rising to the top in the reaction tank;
the ventilation mechanism is used for introducing gas into the culture bin.
Further, the ventilation mechanism comprises an air pump fixedly connected in the base, a surface fixedly connected with a ventilation pipe, and the air pump can be used for introducing air into the culture bin from the outside through the ventilation pipe.
Further, the valve mechanism comprises a first valve mechanism and a second valve mechanism, the first valve mechanism comprises a first valve seat, the first valve seat is fixedly connected in the base, a first through hole I, a first through hole II and a first through hole III are formed in the surface of the first valve seat, a first valve core is rotatably arranged in the first valve seat, the first valve core is used for communicating the first through hole I with the first through hole II, the first valve core is rotated to communicate the first through hole II with the first through hole III, the first through hole I is communicated with the outside through a pipeline, and the first through hole III is communicated with the culture bin;
the surface of the first valve core is fixedly connected with a protruding column I.
Further, the second valve mechanism comprises a second valve seat, the second valve seat is fixedly connected in the base, a first second port and a third second port are formed in the surface of the second valve seat, a second valve core is rotatably arranged in the second valve seat, the first second port is communicated with the second port through the second valve core, the first second port and the third second port can be communicated through rotating the second valve core, and the second port and the third second port are communicated with the culture bin.
Further, the power mechanism comprises a driving mechanism and a synchronous mechanism, the driving mechanism comprises a liquid pump and a spring, a liquid inlet and a liquid outlet of the liquid pump are respectively communicated with a first port II and a second port I, an output shaft of the liquid pump extends out of the surface and is fixedly connected with a threaded rod, a gear and a swinging arm are rotatably arranged in the base, the threaded rod rotates to drive the gear to rotate, the surface of the gear is fixedly connected with a protruding column II, the gear rotates to drive the swinging arm to intermittently reciprocate through the protruding column II, one end of the swinging arm is in sliding connection with the protruding column I, and the swinging arm swings to drive a first valve core to rotate through the protruding column;
one end of the spring is connected with the base, and the other end of the spring is used for driving the swing arm to rotate to the stroke end in the same rotation direction when the rotation stroke of the swing arm exceeds half.
Further, the spraying mechanism comprises a spraying pipe and a drain pipe, the top of the spraying pipe is fixedly connected with a spraying head communicated with the spraying pipe, and the spraying pipe is communicated with a second port III;
the drain pipe is fixedly connected with the culture bin, the culture bin is communicated with the outside, and the mixed liquid in the culture bin is discharged through the drain pipe when the height of the mixed liquid in the culture bin exceeds that of the drain pipe.
Further, the synchronous mechanism comprises two belt pulleys and a belt, the two belt pulleys are respectively and fixedly connected with the first valve core and the second valve core in a coaxial way, the two belt pulleys are connected through belt transmission, and the first valve core and the second valve core are respectively and synchronously rotated through the upper belt pulleys and the belt.
An application of a carbon-fixing photobioreactor for continuously culturing microalgae, comprising the following steps:
s1, firstly, adjusting a valve mechanism to an initial state so that external mixed liquid can be introduced into a culture bin from the bottom of the culture bin through the valve mechanism and a power mechanism;
s2, waiting for a power mechanism to adjust a valve mechanism to enable mixed liquid at the bottom of the culture bin, sending the mixed liquid to the top of the culture bin to spray downwards through the valve mechanism, the power mechanism and a spraying mechanism, simultaneously starting a ventilation mechanism and an illumination lamp plate to enable gases such as carbon dioxide to be introduced into the culture bin from the bottom of the culture bin, and enabling microalgae photosynthesis to absorb carbon dioxide to carry out carbon fixation by illuminating the mixed liquid;
s3, continuously waiting for the power mechanism to adjust the valve mechanism to an initial state, continuously introducing mixed liquid into the culture bin, and allowing excessive mixed liquid to overflow, wherein the power mechanism is used for adjusting the valve mechanism in a reciprocating manner to realize continuous culture.
1. Compared with the prior art, the carbon fixation photobioreactor and the application thereof provided by the invention have the advantages that the light lamp plate, the spraying mechanism, the ventilation mechanism, the power mechanism and the valve mechanism are mutually matched, so that the mixed solution containing microalgae and the culture medium thereof can be sprayed downwards from the top in the culture bin through the spraying mechanism and fully mixed with the introduced gas, the efficiency of absorbing carbon dioxide in the mixed solution through photosynthetic reaction is improved, and the effect of improving the carbon fixation efficiency is achieved.
2. Compared with the prior art, the carbon-fixing photobioreactor and the application thereof provided by the invention have the advantages that the light lamp plate, the spraying mechanism, the ventilation mechanism, the power mechanism and the valve mechanism are matched with each other, so that external culture solution can be intermittently introduced into the culture bin, a part of the cultured redundant mixed solution containing microalgae is discharged, the microalgae is continuously cultured, and the microalgae culture efficiency is improved.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.
FIG. 1 is a schematic diagram of an overall structure according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a whole semi-section provided by an embodiment of the present invention;
FIG. 3 is an enlarged view of portion A of FIG. 2 provided by an embodiment of the present invention;
FIG. 4 is a schematic illustration of a portion of a valve mechanism provided in an embodiment of the present invention;
FIG. 5 is a schematic view of another view of a valve train portion provided in accordance with an embodiment of the present invention;
FIG. 6 is a schematic illustration of a valve train portion in semi-section provided by an embodiment of the present invention;
fig. 7 is a schematic view of a spring and swing arm portion provided in an embodiment of the present invention.
11. A base; 12. a culture bin; 21. a light lamp plate; 31. an air pump; 32. a vent pipe; 41. a first valve seat; 42. a first through hole I; 43. the first through hole II; 44. a first through port III; 45. a first valve core; 46. a first protruding column; 51. a liquid pump; 52. a threaded rod; 53. a gear; 54. a second protruding column; 55. a swing arm; 56. a spring; 61. a second valve seat; 62. a first port; 63. a second port II; 64. a second port III; 65. a second valve core; 71. a pipe; 81. a belt pulley; 82. a belt; 91. a spray tube; 92. a spray head; 93. and (5) a water drain pipe.
Detailed Description
In order to make the technical scheme of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise. Furthermore, the terms "mounted," "connected," "coupled," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1-7, a carbon-fixing photobioreactor and application thereof, the carbon-fixing photobioreactor comprises a reaction tank, a light lamp plate 21, a spraying mechanism and a ventilation mechanism, wherein part of the surface of the reaction tank is transparent, light of the light lamp plate 21 can irradiate the interior of the reaction tank, the reaction tank comprises a base 11, a transparent culture bin 12 is fixedly connected to the surface of the base 11, the light lamp plate 21 is arranged on the outer surface of the culture bin 12, the base 11 is cylindrical, a partition is fixedly connected to the interior of the base 11, a valve mechanism, a power mechanism and a pipeline 71 are arranged in the reaction tank, the valve mechanism is provided with a plurality of through holes, the pipeline 71 is connected with the pipeline 71 and is communicated with the exterior of the reaction tank through the pipeline 71, the power mechanism is used for driving mixed liquid to pass through the valve mechanism, and the valve mechanism is regulated to control the through hole switch, so that the mixed liquid is introduced into the reaction tank from the exterior or circulated in the reaction tank, and the mixed liquid comprises microalgae and culture mediums thereof.
The valve mechanism comprises a first valve mechanism and a second valve mechanism, the first valve mechanism comprises a first valve seat 41, the first valve seat 41 is fixedly connected in the base 11, a first through hole I42, a first through hole II 43 and a first through hole III 44 are formed in the surface of the first valve seat 41, a first valve core 45 is rotatably arranged in the first valve seat 41, the first valve core 45 is used for communicating the first through hole I42 with the first through hole II 43, the first through hole III 44 can be communicated by rotating the first valve core 45, preferably, the first through hole II 43 can be communicated with the first through hole III 44 by rotating the first valve core 45 ninety degrees, the first through hole I42 is communicated with the outside through a pipeline 71, so that external mixed liquid can be communicated with the culture bin 12 through the pipeline 71 and the first through hole III 44 is communicated with the bottom of the culture bin 12 through the pipeline 71; the surface of the first valve core 45 is fixedly connected with a first protruding column 46, the first protruding column 46 is fixedly connected to the position, close to the edge, of the first valve core 45, and the first protruding column 46 is parallel to the axis of the first valve core 45.
The second valve mechanism comprises a second valve seat 61, the second valve seat 61 is fixedly connected in the base 11, a first second port 62, a second port 63 and a third second port 64 are formed in the surface of the second valve seat 61, a second valve core 65 is rotatably arranged in the second valve seat 61, the second valve core 65 is used for communicating the first second port 62 with the second port 63, the second valve core 65 is rotated to enable the first second port 62 to be communicated with the third second port 64, the second valve core 65 is rotated ninety degrees in the same direction as the first valve core 45 to enable the first second port 62 to be communicated with the third second port 64, and the second port 63 and the third second port 64 are communicated with the culture bin 12 and are communicated to the bottom of the culture bin 12 through a pipeline 71. And the liquid inlet and the liquid outlet of the liquid pump 51 are respectively communicated with the first port two 43 and the second port one 62, so that the liquid pump 51 can send the mixed liquid introduced into the first valve seat 41 into the second valve seat 61 through the second port two 43 and the second port one 62.
The spraying mechanism is communicated with the valve mechanism through a port, and the mixed liquid can enter the spraying mechanism through the port and then is sprayed out from the spraying mechanism after rising to the top in the reaction tank; the spraying mechanism comprises a spraying pipe 91 and a water drain pipe 93, the top of the spraying pipe 91 is fixedly connected with a spraying head 92 communicated with the spraying pipe 91, and the spraying pipe 91 is communicated with a second port III 64, so that when the second port I62 is communicated with the second port III 64, the mixed liquid introduced into the second valve seat 61 is sprayed from the top in the culture bin 12 through the spraying pipe 91 and the spraying head 92, the contact area with the gas introduced into the culture bin 12 is increased, the curing efficiency of carbon dioxide is improved, and the heat dissipation is improved;
the drain pipe 93 is fixedly connected with the culture bin 12, the culture bin 12 is communicated with the outside, and when the height of the mixed liquid in the culture bin 12 exceeds the drain pipe 93, the mixed liquid is discharged through the drain pipe 93.
The ventilation mechanism is used for introducing gas into the reaction tank. The ventilation mechanism includes the air pump 31 of fixed connection in base 11, the fixed surface of air pump 31 is connected with breather pipe 32, air pump 31 can follow the outside with gas in cultivateing storehouse 12 through breather pipe 32, the air inlet and a breather pipe 32 fixed connection of air pump 31, and through this breather pipe 32 and outside intercommunication, can extract the inside of self with outside gas such as nitrogen gas, carbon dioxide, the gas outlet of air pump 31 also fixedly connected with breather pipe 32, and through this breather pipe 32 and cultivate storehouse 12 inside intercommunication, and lead to the bottom of cultivateing storehouse 12, can send the gas of sucking into its inside into cultivateing storehouse 12 bottom, make gas get into cultivateing storehouse 12 from the bottom of cultivateing storehouse 12, and the inside of breather pipe 32 that leads to cultivateing storehouse 12 has the check valve, be used for preventing that the breather pipe 32 in cultivateing storehouse 12 from flowing backward to in the air pump 31.
The driving mechanism comprises a driving mechanism and a synchronizing mechanism, the driving mechanism comprises a liquid pump 51 and a spring 56, the extending surface of an output shaft of the liquid pump 51 is coaxially and fixedly connected with a threaded rod 52, a gear 53 and a swinging arm 55 are rotatably arranged in the base 11, the threaded rod 52 rotates to drive the gear 53 to rotate, a boss post II 54 is fixedly connected to the surface of the gear 53, a matching groove is formed in one end of the swinging arm 55, which is close to the boss post II 54, the width of the matching groove is larger than the diameter of the boss post II 54, the boss post II 54 is sleeved in the matching groove, when the gear 53 rotates, the swinging arm 55 is driven to reciprocate through the contact of the boss post II 54 and the edges of the matching groove, the gear 53 rotates to drive the swinging arm 55 to reciprocate intermittently through the boss post II 54, one end of the swinging arm 55 is in sliding connection with the boss post I46, and a sliding groove which is in sliding connection with the boss post I46 is formed in the other end of the swinging arm 55, and the swinging of the swinging arm 55 drives the first valve core 45 to rotate through the boss post I46;
one end of the spring 56 is connected with the base 11, one end of the spring 56 is rotatably connected or fixedly connected with the base 11, the other end of the spring 56 is fixedly connected or rotatably connected with one end of the swing arm 55 close to the first projection column 46, or is directly fixedly connected or rotatably connected with the first projection column 46, and the spring is used for driving the swing arm 55 to rotate to the stroke end in the same rotation direction when the rotation stroke of the swing arm 55 exceeds half, so that the first valve core 45 is quickly rotated into position.
The synchronous mechanism comprises two belt pulleys 81 and a belt 82, the two belt pulleys 81 are respectively and fixedly connected with the first valve core 45 and the second valve core 65 in a coaxial way, the two belt pulleys 81 are connected through the belt 82 in a transmission way, and the first valve core 45 and the second valve core 65 respectively rotate synchronously through the upper belt pulleys 81 and the belt 82, namely, the second valve core 65 rotates by the same angle when the first valve core 45 rotates.
An application of a carbon-fixing photobioreactor for continuously culturing microalgae, comprising the following steps:
s1, firstly, adjusting a valve mechanism to an initial state so that a first port I42 is communicated with a second port II 43, and is communicated with a first port 62 and a second port II 63 through a liquid pump 51, then, communicating a mixed liquid containing microalgae and a culture medium thereof with the first port I42 through a pipeline 71, and starting the liquid pump 51 of a power mechanism, namely, enabling external mixed liquid to be introduced into the culture bin 12 from the bottom of the culture bin 12 through the valve mechanism and the power mechanism;
s2, waiting for a power mechanism to adjust a valve mechanism to enable mixed liquid at the bottom of the culture bin 12, sending the mixed liquid to the top of the culture bin 12 to spray downwards through the valve mechanism, the power mechanism and a spraying mechanism, simultaneously starting a ventilation mechanism and an illumination lamp panel 21 to enable gases such as carbon dioxide and the like to be introduced into the culture bin 12 from the bottom of the culture bin 12, enabling a part of the gases to be mixed with the mixed liquid in the culture bin 12, enabling the rest gases to be mixed with the mixed liquid sprayed downwards through the mixed liquid at the bottom, improving the mixing efficiency, and enabling the light illumination lamp panel 21 to irradiate the mixed liquid to enable microalgae photosynthesis to absorb carbon dioxide in the mixed liquid to carry out carbon fixation;
s3, continuously waiting for the power mechanism to adjust the valve mechanism to an initial state, continuously introducing mixed liquid into the culture bin 12, so that the mixed liquid rises in height and overflows the drain pipe 93 to drain the excessive mixed liquid, and continuously culturing by the power mechanism by reciprocally adjusting the valve mechanism. And the frequency of the valve mechanism can be adjusted by changing gears 53 and threaded rods 52 with different transmission ratios to control the power mechanism.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that modifications may be made to the described embodiments in various different ways without departing from the spirit and scope of the invention. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive of the scope of the invention, which is defined by the appended claims.
Claims (4)
1. A carbon-fixing photobioreactor, characterized in that: the device comprises a reaction tank, a light lamp plate, a spraying mechanism and a ventilation mechanism, wherein the reaction tank comprises a base, the surface of the base is fixedly connected with a transparent culture bin, the light lamp plate is arranged on the outer surface of the culture bin, light of the light lamp plate can irradiate the interior of the culture bin, a valve mechanism, a power mechanism and a pipeline are arranged in the reaction tank, the valve mechanism is provided with a plurality of through holes, the pipeline is connected with the pipeline through the through holes, the power mechanism is used for driving mixed liquid to pass through the valve mechanism and regulating the valve mechanism to control the through hole switch, so that the mixed liquid is introduced into the culture bin from the outside or circulates in the culture bin;
the spraying mechanism is communicated with the valve mechanism through a port, and the mixed liquid can enter the spraying mechanism through the port and then is sprayed out from the spraying mechanism after rising to the top in the reaction tank;
the ventilation mechanism is used for introducing gas into the culture bin;
the valve mechanism comprises a first valve mechanism and a second valve mechanism, the first valve mechanism comprises a first valve seat, the first valve seat is fixedly connected in the base, a first through hole I, a first through hole II and a first through hole III are formed in the surface of the first valve seat, a first valve core is rotatably arranged in the first valve seat, the first valve core is used for communicating the first through hole I with the first through hole II, the first valve core is rotated to communicate the first through hole II with the first through hole III, the first through hole I is communicated with the outside through a pipeline, and the first through hole III is communicated with the culture bin;
the surface of the first valve core is fixedly connected with a protruding column I, the second valve mechanism comprises a second valve seat, the second valve seat is fixedly connected in the base, a second port I, a second port II and a second port III are formed in the surface of the second valve seat, the second valve core is rotatably arranged in the second valve seat, the second valve core is used for communicating the second port I with the second port II, the second valve core is rotated to communicate the second port I with the second port III, and the second port II and the second port III are both communicated with the culture bin;
the driving mechanism comprises a liquid pump and a spring, a liquid inlet and a liquid outlet of the liquid pump are respectively communicated with a first port II and a second port I, an output shaft of the liquid pump extends out of the surface and is fixedly connected with a threaded rod coaxially, a gear and a swinging arm are rotatably arranged in the base, the threaded rod rotates to drive the gear to rotate, a boss column II is fixedly connected with the surface of the gear, the gear rotates to drive the swinging arm to intermittently reciprocate through the boss column II, one end of the swinging arm is in sliding connection with the boss column I, and the swinging arm swings to drive a first valve core to rotate through the boss column;
one end of the spring is connected with the base, the other end of the spring is used for driving the swing arm to rotate to the end of the stroke in the same rotation direction when the rotation stroke of the swing arm exceeds half, the synchronous mechanism comprises two belt pulleys and a belt, the two belt pulleys are respectively and fixedly connected with the first valve core and the second valve core in a coaxial mode, the two belt pulleys are connected through belt transmission, and the first valve core and the second valve core are respectively and synchronously rotated through the upper belt pulley and the belt.
2. A carbon-fixing photobioreactor as claimed in claim 1, wherein: the ventilation mechanism comprises an air pump fixedly connected in the base, a ventilation pipe is fixedly connected to the surface of the air pump, and the air pump can be used for introducing air into the culture bin from the outside through the ventilation pipe.
3. A carbon-fixing photobioreactor as claimed in claim 1, wherein: the spraying mechanism comprises a spraying pipe and a drain pipe, the top of the spraying pipe is fixedly connected with a spraying head communicated with the spraying pipe, and the spraying pipe is communicated with a second port III;
the drain pipe is fixedly connected with the culture bin, the culture bin is communicated with the outside, and the mixed liquid in the culture bin is discharged through the drain pipe when the height of the mixed liquid in the culture bin exceeds that of the drain pipe.
4. Use of a carbon-fixed photobioreactor according to any one of claims 1-3 for continuous cultivation of microalgae, characterized in that: the method comprises the following steps:
s1, firstly, adjusting a valve mechanism to an initial state so that external mixed liquid can be introduced into a culture bin from the bottom of the culture bin through the valve mechanism and a power mechanism;
s2, waiting for a power mechanism to adjust a valve mechanism to enable mixed liquid at the bottom of the culture bin, sending the mixed liquid to the top of the culture bin to spray downwards through the valve mechanism, the power mechanism and a spraying mechanism, simultaneously starting a ventilation mechanism and an illumination lamp plate to enable gases such as carbon dioxide to be introduced into the culture bin from the bottom of the culture bin, and enabling microalgae photosynthesis to absorb carbon dioxide to carry out carbon fixation by illuminating the mixed liquid;
s3, continuously waiting for the power mechanism to adjust the valve mechanism to an initial state, continuously introducing mixed liquid into the culture bin, and allowing excessive mixed liquid to overflow, wherein the power mechanism is used for adjusting the valve mechanism in a reciprocating manner to realize continuous culture.
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