CN116395734B - Method for preparing nano copper oxide particles by using hibiscus flower extract, product and application - Google Patents
Method for preparing nano copper oxide particles by using hibiscus flower extract, product and application Download PDFInfo
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- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 title claims abstract description 65
- 239000005751 Copper oxide Substances 0.000 title claims abstract description 50
- 229910000431 copper oxide Inorganic materials 0.000 title claims abstract description 50
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- 235000005206 Hibiscus Nutrition 0.000 title claims abstract description 36
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- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
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- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
- C01G3/00—Compounds of copper
- C01G3/02—Oxides; Hydroxides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C01P2002/80—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70
- C01P2002/84—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70 by UV- or VIS- data
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses a method for preparing nanometer copper oxide particles by using hibiscus flower extract, a product and application thereof, belonging to the technical field of green biosynthesis nanometer materials, wherein the method comprises the following steps: (1) Pretreating flos Hibisci petal, grinding into powder, mixing with deionized water, extracting at 55-75deg.C for 3-6 hr, and filtering to obtain flos Hibisci extractive solution; (2) Mixing the hibiscus flower extract with the CuO suspension for reaction, and then centrifuging, washing and vacuum freeze-drying the obtained mixed solution to obtain the nano copper oxide particles. The preparation process has simple steps, mild reaction conditions and environmental protection, and the obtained nano copper oxide strip-shaped particles have uniform size and smaller size, can effectively inhibit the activity of plant pathogenic bacteria such as bacterial blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) of rice and the like, and prevent and treat bacterial diseases of the rice.
Description
Technical Field
The invention belongs to the technical field of green biosynthesis nanometer materials, and particularly relates to a method for preparing nanometer copper oxide particles by using hibiscus flower extract, a product and application thereof.
Background
The nano material generally refers to a material with a dimension of 1-100nm, and has wide application prospect and huge application potential in the fields of chemical industry, medicine, food packaging, environmental technology, sensing and the like due to the small-size effect, quantum size effect, surface effect, volume effect and the like. In terms of nano bactericidal materials, nano silver-based materials have been widely studied, but silver-based materials are expensive. The nano-copper-based material is relatively cost effective and is also an excellent antimicrobial agent, and previous studies have reported that nano-copper oxide particles (CuONPs) are transported on corn plants through xylem and phloem tissue.
Common preparation methods of nano copper oxide include precipitation method, solid phase synthesis method, hydrothermal method, electrochemical deposition method and the like, but the synthesis methods generally require longer time, consume higher energy and have lower antibacterial performance of the nano copper oxide. The green synthesis of nano metal material with plant extract is a research hotspot in the prior art, and the main principle of synthesis is that substances such as phenolic compounds, terpenoid compounds, alkaloids, coenzyme and the like contained in plant extract can be used as a stabilizer to prepare nano particles. The synthesis process is green, environment-friendly and efficient, and the plant green synthesis of the nano copper oxide is gradually replaced by the traditional synthesis method, so that the method becomes a production method with wide prospects.
The Chinese patent document with publication number CN114368777A discloses a method for green synthesis of nano copper oxide by using galangal extracting solution, which comprises the following steps: mixing rhizoma Alpiniae Officinarum with solvent, heating, and filtering to obtain rhizoma Alpiniae Officinarum extractive solution; mixing galangal extracting solution with precursor solution containing copper ions to react to obtain nano copper suspension, centrifuging the nano copper suspension to obtain nano copper particles, and further washing, drying, calcining and grinding to obtain nano copper oxide, wherein the process conditions are harsh; and fewer plant species are disclosed in the prior art as being useful for synthesizing nano-copper oxide particles.
In addition, the bacterial diseases of the rice are main diseases in the growth process of the rice, and huge losses of agricultural economy in China are easily caused. At present, chemical control is still mainly used for controlling bacterial diseases of rice, however, the traditional chemical pesticides easily cause the problems of bacterial resistance, environmental pollution and the like, and can cause potential harm to human bodies. Therefore, it is necessary to develop a novel green method for controlling bacterial diseases of rice.
Disclosure of Invention
The invention provides a method for preparing nano copper oxide particles by using hibiscus flower extract, which has the advantages of simple preparation process steps, mild reaction conditions, environmental protection, uniform size of obtained nano copper oxide strip particles, smaller size, good antibacterial effect, and particularly excellent stability and dispersibility, and can effectively inhibit the activity of plant pathogenic bacteria such as rice bacterial blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) and the like.
The technical scheme adopted is as follows:
A method for preparing nanometer copper oxide particles by using hibiscus flower extract comprises the following steps:
(1) Pretreating flos Hibisci petal, grinding into powder, mixing with deionized water, extracting at 55-75deg.C for 2-6 hr, and filtering to obtain flos Hibisci extractive solution;
(2) Mixing the hibiscus flower extract with the CuO suspension for reaction, and then centrifuging, washing and vacuum freeze-drying the obtained mixed solution to obtain the nano copper oxide particles (CuONPs).
The hibiscus flower belongs to the hibiscus genus of the malvaceae family, is widely distributed in China, has a plurality of cultivated varieties, has a gorgeous flower shape, and is mostly applied to landscaping in gardens. The hibiscus flower is mostly used as a vegetable raw material to be cooked into dishes in Zhejiang folk, and has smooth mouthfeel and unique flavor. Meanwhile, the hibiscus flower gene is rich in flavonoids and has medicinal value, and has the effects of resisting cancer, preventing cardiovascular diseases and the like. Flavonoid is also a natural strong antioxidant, has no pollution, little toxicity and few side effects, and has very outstanding effects on the treatment of diseases and the health care of human bodies.
In the method, hibiscus flower for ornamental is selected as a material, and CuONPs is prepared by using petal extract. The plant chemical components of the hibiscus flower extract comprise flavonoids, tannins, alkaloids, steroids, terpenes, amino acids, glycosides, mucilage, gum and the like, part of the plant chemical components play a role of stabilizing agent, part of the plant chemical components can interact with CuO, support the growth of strip-shaped nano structures and synthesize nano copper oxide strip-shaped particles.
Experiments show that the nano copper oxide particles obtained by the method have stable structure and certain control effect on bacterial diseases of rice, particularly have strong inhibition effect on bacterial blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) of rice, and have wide application prospect.
Preferably, the pretreatment step includes a dust removal treatment and a drying treatment.
Grinding the pretreated petals of hibiscus flower into powder by using a grinder, wherein the grinder adopts a program of 65-90Hz for 90-120s. Too low a frequency results in a low extraction efficiency of the plant material and too high a frequency results in unnecessary energy consumption.
Preferably, in the step (1), the mass ratio of the hibiscus flower petal powder to deionized water is 1:90-100.
In the step (2), the mixing reaction condition of the hibiscus flower extract and the CuO suspension is as follows: the temperature is 55-75 ℃ and the time is 3-6h. The temperature setting is too low, so that insufficient reaction and poor synthesis effect are caused, and finally the particle size, stability and uniformity of the nano copper oxide particles are influenced, so that the antibacterial effect is influenced; too high a temperature setting can easily lead to deterioration of the composition and also to unnecessary energy consumption.
Preferably, the concentration of the CuO suspension is 0.16-0.24mg/mL, and the volume ratio of the hibiscus flower extract to the CuO suspension is 1-3:1. a too high content of the effective components of hibiscus flower can affect the generation of nano copper oxide particles, and a too low content of the effective components can lead to the reduction of the synthesis efficiency of the final product.
Preferably, the centrifugation speed is 10000-14000rpm, the centrifugation time is 8-15min, too low centrifugation speed and too short centrifugation time can cause the supernatant to be not clear, so that the finally obtained nano copper oxide particles have less content and more impurities, and the antibacterial effect of the product is further affected.
The invention also provides the nano copper oxide particles prepared by the method for preparing the nano copper oxide particles by using the hibiscus flower extract, wherein the nano copper oxide particles are in a nano strip shape, have the particle size of 20-50nm, and have stable structure, small size and uniform size.
The invention also provides application of the nano copper oxide particles in inhibiting plant pathogenic bacteria, preferably comprising bacterial leaf blight of rice (Xanthomonas oryzae, xoo).
The invention also provides application of the nano copper oxide particles as agricultural bactericides in preventing and controlling plant diseases caused by plant pathogenic bacteria, wherein the plant diseases comprise rice bacterial diseases such as rice bacterial leaf blight and the like. Experiments show that the nano copper oxide particles prepared by the method have a strong antibacterial effect on bacterial blight (Xanthomonas oryzae pv. Oryzae, xoo) of rice, can control diseases such as bacterial blight of rice caused by the bacterial blight, can replace the traditional chemical antibacterial agent, and reduces economic loss caused by the diseases.
The specific application method for preventing and treating plant diseases comprises the following steps: the nano copper oxide particles are dissolved in water to prepare CuONPs suspension, and CuONPs suspension is uniformly sprayed on the plants to be controlled.
Preferably, the concentration of CuONPs suspension is 50-200 mug/mL, and the agricultural bactericide in the preferred range has good control effect on rice bacterial diseases such as rice bacterial leaf blight and the like, and further improves the concentration and does not improve much.
Compared with the prior art, the invention has the beneficial effects that:
(1) The nano copper oxide particles obtained by the invention have stable structure, small average particle diameter and uniform size, can effectively inhibit the activity of plant pathogenic bacteria such as rice bacterial leaf blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) and the like, and have a very strong sterilization effect;
(2) The process of the invention has relatively less consumption, does not generate toxic byproducts, is very environment-friendly and safe, belongs to a green synthesis process, has simple process, does not need to add additional catalyst, has mild reaction conditions, and is suitable for large-scale production;
(3) The CuONPs obtained by the invention is used as an agricultural antibacterial agent for preventing and treating rice bacterial diseases, has good preventing and treating effects, can not bring potential harm to human bodies, can not pollute the environment, is simple and easy to apply, and has wide application prospects in the field of agricultural production.
Drawings
FIG. 1 is a diagram showing the ultraviolet-visible spectrum of CuONPs synthesized by using the extract of hibiscus flower.
A in FIG. 2 is an infrared spectrum of CuONPs obtained in example 1, B is a scanning electron microscope of CuONPs, and C is an X-ray diffraction of CuONPs.
FIG. 3 is an EDS diagram of CuONPs produced in example 1.
Fig. 4 is a graph showing the bacteriostatic effect of suspensions of different concentrations CuONPs on bacterial blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) of rice, wherein a is an optical picture of a zone of inhibition, B is a statistical chart of diameter change of the zone of inhibition, and C is a chart of absorbance change, and in the graph, a, B, C and d represent that the difference between different treatments at 5% level is significant.
FIG. 5 is a graph showing the effect of suspensions of various concentrations CuONPs on the formation of bacterial blight (Xanthomonas oryzae pv. Oryzae, xoo) biofilm; in the figure, a, b, c represent a significant difference at the 5% level between the different treatments.
FIG. 6 is a graph showing the result of staining cells of rice bacterial leaf blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) treated with CuONPs suspension, wherein A is a negative control, and B is cells treated with CuONPs suspension; in the figure, B++ represents the ratio of live bacteria to dead bacteria.
A, B, C in FIG. 7 is a graph showing the effect of CuONPs suspension on destroying cells of bacterial blight of rice (Xanthomonas oryzae pv. Oryzae, xoo) with time, with the treatment time of A being the shortest, B times, and C being the longest.
FIG. 8 is a graph showing the statistical results of RNA extravasation of intracellular lysates after treatment of bacterial blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) of rice for 24h with suspensions of different concentrations CuONPs, where a, b, c, d represent significant differences at 5% levels between the different treatments.
Detailed Description
The invention is further elucidated below in connection with the examples and the accompanying drawing. It is to be understood that these examples are for illustration of the invention only and are not intended to limit the scope of the invention.
Bacterial leaf blight of rice (Xanthomonas oryzae pv. Oryzae, xoo), which is isolated from diseased rice leaves in Jin Huashi field in Zhejiang province.
The CuO suspension is prepared by dissolving copper oxide in deionized water, wherein the granularity of the copper oxide is 100 meshes.
Example 1
(1) Washing and drying newly picked petals of hibiscus syriacus flower, grinding the petals into powder by a grinder under the procedures of 90Hz and 90s, weighing 2g of hibiscus syriacus flower powder, mixing the powder with 200mL of deionized water, placing the mixture in a water bath kettle which is heated to 55 ℃ for extraction for 5 hours, intermittently stirring the mixture by a glass rod in the process, cooling the mixture at room temperature, and fully filtering to remove solid impurities to obtain hibiscus syriacus flower extract;
(2) Mixing 200mL of the hibiscus flower extract obtained in the step (1) with 200mL of CuO suspension with the concentration of 0.16mg/mL, placing the mixture into a water bath kettle with the temperature of 55 ℃, stirring once every 15 minutes, taking out the mixed solution after 4 hours, centrifuging the mixed solution for 8 minutes at the speed of 14000rpm, removing the supernatant, taking out the precipitate, washing, and performing vacuum freeze drying to obtain powdery nano copper oxide particles.
The structural features and physicochemical properties of CuONPs were evaluated using ultraviolet visible absorption spectroscopy (UV-VIS), scanning Electron Microscopy (SEM), energy spectroscopy (EDS), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD).
FIG. 1 is an ultraviolet-visible spectrum of CuONPs synthesized by using hibiscus flower extract, and the result shows that the hibiscus flower extract has an absorption peak in the range of 255-275nm, and the synthesized CuONPs has a surface plasmon resonance peak at 255 nm.
A in fig. 2 is an infrared spectrum of CuONPs prepared in example 1, from which it is known that CuONPs has been successfully synthesized; the scanning electron microscope image with B being CuONPs shows that the synthesized CuONPs is a strip particle, the particle size is 20-50nm, and the X-ray diffraction image with C being CuONPs shows that the characteristic peaks of (110), (002), (200), (202), (113) and (331) respectively appear in CuONPs, and the characteristic peaks are consistent with the characteristic peaks of the CuO powder diffraction image, so that stable CuONPs is synthesized.
FIG. 3 is an EDS diagram of CuONPs obtained in example 1, the nano-copper oxide particles mainly consist of two elements of Cu and O, the Cu element accounts for 77.68%, and the O element accounts for 22.23%.
Example 2
(1) Washing and drying newly picked petals of the hibiscus syriacus flower, grinding the petals into powder by a grinder under the procedure of 85Hz and 110s, weighing 2g of the hibiscus syriacus flower leaves, mixing the hibiscus syriacus flower leaves with 190mL of deionized water, placing the hibiscus syriacus flower leaves in a water bath kettle which is heated to 60 ℃ for extraction for 4 hours, intermittently stirring the hibiscus syriacus flower leaves by a glass rod in the process, cooling the hibiscus syriacus flower leaves at room temperature, and fully filtering to remove solid impurities to obtain hibiscus syriacus flower extract;
(2) Mixing 300mL of the hibiscus flower extract obtained in the step (1) with 200mL of CuO suspension with the concentration of 0.2mg/mL, placing the mixture into a water bath kettle with the temperature of 60 ℃, stirring once every 15 minutes, taking out the mixed solution after 4 hours, centrifuging the mixed solution for 10 minutes at the speed of 12000rpm, removing the supernatant, taking out the precipitate, washing, and performing vacuum freeze drying to obtain powdery nano copper oxide particles.
Example 3
(1) Washing and drying newly picked petals of hibiscus syriacus flower, grinding the petals into powder by a grinder under the procedures of 80Hz and 100s, weighing 2g of hibiscus syriacus flower powder, mixing the powder with 180mL of deionized water, placing the mixture in a water bath kettle which is heated to 65 ℃ for extraction for 3 hours, intermittently stirring the mixture by a glass rod in the process, cooling the mixture at room temperature, and fully filtering to remove solid impurities to obtain hibiscus syriacus flower extract;
(2) Mixing 400mL of the hibiscus flower extract obtained in the step (1) with 200mL of CuO suspension with the concentration of 0.24mg/mL, placing the mixture into a water bath kettle with the temperature of 65 ℃ for stirring once every 15 minutes, taking out the mixed solution after 4 hours, centrifuging the mixed solution for 15 minutes at the rotating speed of 10000rpm, removing the supernatant, taking out the precipitate, washing and carrying out vacuum freeze drying to obtain powdery nano copper oxide particles.
Sample analysis
And (3) evaluating antibacterial effect:
effect of CuONPs suspension concentration on bacteriostatic Effect
To evaluate CuONPs for inhibition of bacterial blight of rice (Xanthomonas oryzae pv. Oryzae, xoo), cuONPs powder prepared in example 1 was prepared as 50, 100, 200 μg/mL solutions, respectively, and the inhibition performance of different CuONPs suspension concentrations was evaluated by measuring the absorbance of the bacterial solution and the diameter of the plate inhibition zone.
As can be seen from A and B in FIG. 4, after the plates are placed under proper conditions for culturing for 24 hours, compared with the control group, the maximum inhibition zone on the plates added with CuONPs suspension can reach 39mm; as is clear from C in FIG. 4, when the treatment group and the control group were cultured under appropriate conditions for 24 hours, the minimum and maximum growth inhibition rates of CuONPs suspensions of 50, 100 and 200. Mu.g/mL were 36.12% and 79.65%, respectively, and the higher the concentration was, the better the bacteriostatic effect was, but when the concentration was further increased on the basis of 200. Mu.g/mL, the bacteriostatic effect was not significantly increased.
As can be seen from fig. 4, the higher the concentration of CuONPs suspension, the better the antibacterial effect, and the CuONPs synthesized by using hibiscus flowers can significantly inhibit the growth of bacterial blight bacteria (Xoo) of rice.
(II) Effect of CuONPs suspension concentration on plant pathogenic biofilm formation
The biological membrane can resist the inhibition of bacteria by antibacterial substances from plants by improving the viability of the bacteria under severe conditions and promote the colonization of the host plants by the bacteria, thereby playing an important role in the virulence of plant pathogenic bacteria.
To fully understand the effect of CuONPs synthesized in example 1 on the biofilm formation of bacterial blight bacteria (Xanthomonas oryzae pv. Oryzae, xoo) of rice, cuONPs suspensions with concentrations of 50, 100 and 200 μg/mL were added to pathogenic bacteria solutions with equal OD600, respectively, and after 24 hours of incubation under appropriate conditions with equal volumes of bacteria solution as a blank, the effect of nano copper oxide on the biofilm formation was monitored by measuring the OD570 value of the biofilm formation.
FIG. 5 is a graph showing the effect of suspensions of various concentrations CuONPs on the formation of bacterial blight (Xanthomonas oryzae pv. Oryzae, xoo) biofilm; from this figure, it was found that the amount of biofilm formation by pathogenic bacteria gradually decreased with increasing concentration of nano copper oxide particles, and that the amount of biofilm formation decreased by 79.17% after treatment with a suspension having a concentration of 200. Mu.g/mL CuONPs. It is shown from this that the nano copper oxide particles inhibit the formation of plant pathogenic biofilm and thus reduce the survival and colonization ability of pathogenic bacteria.
Bacteriostatic effect of CuONPs suspension
To further demonstrate that CuONPs is indeed capable of causing bacterial cell structure to die, thus exerting a good bacteriostatic effect, the CuONPs powder synthesized in example 1 was formulated with water as a 200 μg/mL suspension. After the CuONPs suspension is reacted with bacterial blight of rice (Xanthomonas oryzae pv. Oryzae, xoo) for 8 hours, the obtained suspension is observed under a flow cytometer, and a sample treated by double distilled water is taken as a negative control, and the obtained result diagram is shown in fig. 6, wherein dead cells can allow PI (propidium iodide) to enter cell nuclei to dye the cell nuclei into red, and the PI cannot dye the cell nuclei of living cells.
A in fig. 6 is a negative control, and the apoptosis rate is 2.02%; b in FIG. 6 is a CuONPs suspension treated cell, and it can be seen that the pathogenic bacteria cells are significantly reduced after 200 μg/mL CuONPs suspension treatment, and the apoptosis rate is 99.42%, which indicates that CuONPs can inhibit the growth of cells.
In addition, the cell damage degree can be more intuitively seen through the transmission electron microscope. FIG. 7 shows A, B, C shows that when rice bacterial blight (Xanthomonas oryzae pv. Oryzae, xoo) is destroyed by CuONPs suspension at a concentration of 200 μg/mL, nanoparticles enter intact and normal cells as seen in panel A, and two panels B, C show that bacterial cells are treated with nano copper oxide, which destroys the cell structure and causes the cells to assume an abnormal state and thus cause cell death.
(IV) influence of CuONPs on extravasation of phytopathogenic substances
The bactericidal capacity of the synthesized CuONPs was evaluated by measuring the amount of endo-lysate extravasation of bacterial blight of rice (Xanthomonas oryzae pv. Oryzae, xoo) treated with suspensions of different concentrations CuONPs.
As can be seen from FIG. 8, the maximum extravasation of the intracellular lysate was observed at a concentration of CuONPs suspension of 200. Mu.g/mL, indicating that the cellular structure of the Xoo strain was destroyed to the greatest extent. As CuONPs suspension concentration decreased, its intracellular content of RNA and DNA gradually decreased, indicating that the cells were destroyed.
From this, cuONPs was found to have an effect on extravasation of the endopolyte of the plant pathogen, indicating that it is able to disrupt the cellular structure of the plant pathogen.
Application example
The CuONPs powder obtained in example 1 was weighed in a greenhouse or field and dissolved in 10L of water to prepare a CuONPs suspension of 200. Mu.g/mL, and the suspension was uniformly sprayed on the rice which is a possible disease, and the crops sprayed with the CuONPs suspension were observed to grow better than those not sprayed, thus it could be demonstrated that the CuONPs suspension was effective in disease control of crops.
While the foregoing embodiments have been described in detail in connection with the embodiments of the invention, it should be understood that the foregoing embodiments are merely illustrative of the invention and are not intended to limit the invention, and any modifications, additions, substitutions and the like made within the principles of the invention are intended to be included within the scope of the invention.
Claims (4)
1. The application of the nano copper oxide particles in inhibiting plant pathogenic bacteria is characterized in that the plant pathogenic bacteria are rice bacterial leaf blight bacteria;
The preparation method of the nano copper oxide particles comprises the following steps:
(1) Pretreating flos Hibisci petal, grinding into powder, mixing with deionized water, extracting at 55-75deg.C for 2-6 hr, and filtering to obtain flos Hibisci extractive solution;
(2) Mixing the hibiscus flower extract with CuO suspension for reaction, and centrifuging, washing and vacuum freeze-drying the obtained mixed solution to obtain the nano copper oxide particles;
in the step (1), the mass ratio of the hibiscus flower petal powder to the deionized water is 1:90-100;
in the step (2), the mixing reaction condition of the hibiscus flower extract and the CuO suspension is as follows: the temperature is 55-75 ℃ and the time is 3-6 h;
The concentration of the CuO suspension is 0.16-0.24mg/mL, and the volume ratio of the hibiscus flower extract to the CuO suspension is 1-3:1, a step of;
The centrifugation speed is 10000-14000rpm, and the centrifugation time is 8-15min;
The nanometer copper oxide particles are in a nanometer strip shape, and the particle size is 20-50nm.
2. The use of nano-copper oxide particles according to claim 1 for inhibiting plant pathogenic bacteria, wherein the pretreatment step comprises a dust removal treatment and a drying treatment.
3. The use of nano copper oxide particles according to claim 1 for inhibiting plant pathogenic bacteria, wherein the pretreated petals of hibiscus flower are ground into powder by using a grinder, and the grinder is selected from the procedures of 65-90hz and 90-120s.
4. The application of the nano copper oxide particles as agricultural bactericides in preventing and controlling plant diseases caused by plant pathogenic bacteria is characterized in that the plant pathogenic bacteria are bacterial leaf blight of rice, and the plant diseases are bacterial diseases of rice;
The preparation method of the nano copper oxide particles comprises the following steps:
(1) Pretreating flos Hibisci petal, grinding into powder, mixing with deionized water, extracting at 55-75deg.C for 2-6 hr, and filtering to obtain flos Hibisci extractive solution;
(2) Mixing the hibiscus flower extract with CuO suspension for reaction, and centrifuging, washing and vacuum freeze-drying the obtained mixed solution to obtain the nano copper oxide particles;
in the step (1), the mass ratio of the hibiscus flower petal powder to the deionized water is 1:90-100;
in the step (2), the mixing reaction condition of the hibiscus flower extract and the CuO suspension is as follows: the temperature is 55-75 ℃ and the time is 3-6 h;
The concentration of the CuO suspension is 0.16-0.24mg/mL, and the volume ratio of the hibiscus flower extract to the CuO suspension is 1-3:1, a step of;
The centrifugation speed is 10000-14000rpm, and the centrifugation time is 8-15min;
The nanometer copper oxide particles are in a nanometer strip shape, and the particle size is 20-50nm.
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| CN109678197A (en) * | 2018-12-11 | 2019-04-26 | 浙江大学 | A kind of method preparing nano-ZnO using plant extraction liquid and products thereof and application |
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| CN109678197A (en) * | 2018-12-11 | 2019-04-26 | 浙江大学 | A kind of method preparing nano-ZnO using plant extraction liquid and products thereof and application |
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