CN116373367B - Preparation method and application of cervical biological sample self-sampling device - Google Patents
Preparation method and application of cervical biological sample self-sampling device Download PDFInfo
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- 238000005070 sampling Methods 0.000 title claims abstract description 86
- 239000012472 biological sample Substances 0.000 title claims abstract description 85
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000005468 ion implantation Methods 0.000 claims abstract description 32
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000000741 silica gel Substances 0.000 claims abstract description 27
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 27
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- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 30
- 229910052697 platinum Inorganic materials 0.000 claims description 15
- 239000003054 catalyst Substances 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 9
- 238000007400 DNA extraction Methods 0.000 claims description 6
- 210000004209 hair Anatomy 0.000 claims description 3
- 230000011987 methylation Effects 0.000 claims description 3
- 238000007069 methylation reaction Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 12
- 230000021164 cell adhesion Effects 0.000 abstract description 10
- 239000012620 biological material Substances 0.000 abstract description 2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C71/00—After-treatment of articles without altering their shape; Apparatus therefor
- B29C71/04—After-treatment of articles without altering their shape; Apparatus therefor by wave energy or particle radiation, e.g. for curing or vulcanising preformed articles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29D—PRODUCING PARTICULAR ARTICLES FROM PLASTICS OR FROM SUBSTANCES IN A PLASTIC STATE
- B29D99/00—Subject matter not provided for in other groups of this subclass
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- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C14/00—Coating by vacuum evaporation, by sputtering or by ion implantation of the coating forming material
- C23C14/22—Coating by vacuum evaporation, by sputtering or by ion implantation of the coating forming material characterised by the process of coating
- C23C14/48—Ion implantation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B2010/0216—Sampling brushes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The utility model discloses a preparation method and application of a cervical biological sample self-sampling device, and belongs to the technical field of biological materials. The preparation method comprises the steps of placing silica gel into a mold, heating at 100-150 ℃, demolding, and performing carbon ion implantation by taking graphite as a carbon ion source to obtain the cervical biological sample self-sampling device. The cervical biological sample self-sampling device prepared by the utility model is only 3-5 mm thick and has good mechanical properties, on one hand, the self-sampling difficulty can be reduced, and on the other hand, the cervical biological sample self-sampling device cannot be broken due to use. After being modified by carbon ion implantation, the method has better cell adhesion capability and antibacterial capability, and can increase the collected biological sample amount, so that the detection result is more accurate.
Description
Technical Field
The utility model belongs to the technical field of biological materials, and particularly relates to a preparation method and application of a cervical biological sample self-sampling device.
Background
Cervical cancer is one of the most common malignant tumors in women, 90% of which are caused by Human Papillomavirus (HPV), which is a dependent HPV cervical cancer; HPV screening, discovery and treatment of pre-cervical lesions is critical in reducing cervical cancer morbidity and mortality. 10% of cervical cancers are independent HPV cervical cancers, and in recent years, detection of DNA methylation in cervical exfoliated cells can well judge cervical states, and particularly has high clinical value for detection of independent HPV cervical cancers.
The conventional cervical screening is mainly carried out in hospitals, a professional medical staff takes a sterile vaginal dilator to expose the vagina and the cervix completely, after disinfecting the vagina and the cervix, a special soft brush is used for taking secretion of the cervix, the secretion is placed in a sample preservation solution to be detected, and after a certain detection period, a patient goes to the hospitals to take out the detection result. Along with national popularization of cervical cancer screening, the current sampling method causes workload to medical staff and hospitals.
In recent years, with the progress of detection technology, a third-party medical inspection institute can directly provide detection service for individual clients, a subject can finish detection by means of express delivery and self-sampling, and a report is remotely checked through the Internet, so that the privacy of the subject and the report acquisition mode are simpler and more convenient.
However, the self-sampling swab in the current market is similar to a medical swab in structure, so that a subject cannot perceive a cervical orifice, and is unfavorable for taking cervical orifice exfoliated cells.
Disclosure of Invention
In order to solve at least one of the technical problems, the utility model adopts the following technical scheme:
the utility model provides a preparation method of a cervical biological sample self-sampling device, which comprises the steps of putting silica gel into a mold, heating at 100-150 ℃, demolding, taking graphite as a carbon ion source, and performing carbon ion implantation to obtain the cervical biological sample self-sampling device, wherein the cervical biological sample self-sampling device comprises a sleeve piece for sleeving a hand and a brush head fixedly arranged at the top end of the sleeve piece; the brush head comprises a main peak part positioned at the center of the top end of the sleeve member and a plurality of brush hairs which are arranged outside the main peak part and lower than the main peak part.
In some embodiments of the utility model, 0.3% -0.8% platinum catalyst is added before and/or during heating.
In some embodiments of the utility model, the platinum catalyst has a platinum content of 5000ppm.
In some embodiments of the utility model, the cervical biological sample self-sampling device has a thickness of 3-5 mm. The silica gel used for preparing the cervical biological sample self-sampling device can still keep good mechanical properties when the silica gel is 3-5 mm thick after screening and optimizing, so that a user can easily feel a sampling position (cervical orifice) when self-sampling is performed, and the cervical biological sample self-sampling device cannot be broken due to use.
In some embodiments of the utility model, the brush head of the cervical biological sample self-sampling device is subjected to carbon ion implantation. When the cervical biological sample self-sampling device is used for sampling, the brush head is mainly used for sampling, so that under some preferred conditions, the purpose can be achieved only by carrying out carbon ion implantation on the brush head.
In some embodiments of the utility model, the carbon ion implant dose is 1×10 15 ~5×10 16 icos／cm 2 . Experiments prove that the carbon ions are injected to modify the brush head of the cervical biological sample self-sampling device, so that the cell adhesion capability and the antibacterial capability of the brush head can be remarkably improved. Most importantly, the inventor finds that after carbon ions are injected into the surface of the silica gel, the original smooth surface of the silica gel is changed in appearance, and the change does not influence the physical strength of the silica gel, but improves the hydrophilicity of the silica gel, so that the acquired biological sample size can be increased, and the detection result is more accurate.
In the utility model, the cervical biological sample self-collecting device after carbon ion implantation has better cell adhesion capability than the cervical biological sample self-collecting device without carbon ion implantation, and the larger the carbon ion dosage during implantation, the better the effect. But the carbon ion dose increases to a certain extent (1×10) 16 icos／cm 2 ) After that, the cell adhesion capacity no longer increases significantly. And the cervical biological sample self-collecting device after carbon ion implantation has better antibacterial capacity than the cervical biological sample self-collecting device without carbon ion implantation, and the larger the carbon ion dosage during implantation, the better the antibacterial effect. But the carbon ion dose increases to a certain extent (1×10) 16 icos／cm 2 ) After that, the bacteriostatic ability is no longer significantly increased. Thus, in some embodiments of the utility model, the carbon ion implant dose is 5 x 10 15 ~1×10 16 icos／cm 2 。
The second aspect of the utility model provides a cervical biological sample self-sampling device prepared by the preparation method according to any one of the first aspect of the utility model.
A third aspect of the present utility model provides the use of the cervical biological sample self-collection device according to the second aspect of the present utility model for preparing a kit for self-collection of a cervical biological sample by a subject in a cervical biological sample detection kit.
The kit may replace any device or apparatus and equipment used in medicine to collect cervical biological samples, such as swabs.
In some embodiments of the utility model, the kit further comprises DNA extraction reagents.
In some embodiments of the utility model, the kit further comprises a methylation detection reagent and/or an HPV detection reagent.
In some embodiments of the utility model, a reagent or combination of reagents capable of eluting or detaching a collected biological sample from the cervical biological sample collection device is also included.
In the present utility model, the cervical biological sample includes, but is not limited to, cervical cells, infectious agents, or other endogenous or exogenous cells or cell fragments or cell secretions. In some embodiments of the utility model, the infectious agent includes, but is not limited to, HPV viruses and/or host cells containing HPV viruses, bacteria, and the like.
The beneficial effects of the utility model are that
Compared with the prior art, the utility model has the following beneficial effects:
the silica gel used for preparing the cervical biological sample self-sampling device can still keep good mechanical properties, such as good extensibility, when the thickness of the silica gel is 3-5 mm after screening and optimizing. On the one hand, the utility model can make the user easily feel the sampling position (cervical orifice) when self-sampling and bend along with the fingers, thus greatly reducing the sampling difficulty in the self-sampling process. On the other hand, the utility model is not broken due to use.
According to the cervical biological sample self-sampling device, the brush head of the cervical biological sample self-sampling device is modified by injecting carbon ions, so that the cell adhesion capacity and the antibacterial capacity of the brush head can be remarkably improved, and after the carbon ions are injected into the surface of the silica gel, the original smooth surface of the silica gel is changed in appearance, the physical strength of the silica gel is not affected by the change, the hydrophilicity of the silica gel is improved, the collected biological sample quantity can be increased, and the detection result is more accurate.
Drawings
Fig. 1 shows a schematic view of the brush head of the cervical biological sample self-sampling device of the present utility model.
Fig. 2 shows a schematic view of the entire cervical biological sample self-collection device of the present utility model.
Fig. 3 shows a schematic view of a brush head of another cervical biological sample self-sampling device of the present utility model.
Fig. 4 shows a schematic view of the whole of another cervical biological sample self-collection device according to the utility model.
FIG. 5 shows the effect of varying carbon ion implant doses on total DNA extraction.
Fig. 6 shows the effect of varying carbon ion implant doses on bacteriostatic ability.
Detailed Description
Unless otherwise indicated, implied from the context, or common denominator in the art, all parts and percentages in the present utility model are based on weight and the test and characterization methods used are synchronized with the filing date of the present utility model. Where applicable, the disclosure of any patent, patent application, or publication referred to in this application is incorporated by reference in its entirety, and the equivalent patents to those cited in this application are incorporated by reference, particularly as if they were set forth in the relevant terms of art. If the definition of a particular term disclosed in the prior art is inconsistent with any definition provided in the present utility model, the definition of the term provided in the present utility model controls.
The numerical ranges in the present utility model are approximations, so that it may include the numerical values outside the range unless otherwise indicated. The numerical range includes all values from the lower value to the upper value that increase by 1 unit, provided that there is a spacing of at least 2 units between any lower value and any higher value. For ranges containing values less than 1 or containing fractions greater than 1 (e.g., 1.1,1.5, etc.), then 1 unit is suitably considered to be 0.0001,0.001,0.01, or 0.1. For a range containing units of less than 10 (e.g., 1 to 5), 1 unit is generally considered to be 0.1. These are merely specific examples of what is intended to be provided, and all possible combinations of numerical values between the lowest value and the highest value enumerated are to be considered to be expressly stated in this disclosure.
The terms "comprises," "comprising," "including," and their derivatives do not exclude the presence of any other component, step or process, and are not related to whether or not such other component, step or process is disclosed in the present utility model. For the avoidance of any doubt, all use of the terms "comprising", "including" or "having" herein, unless expressly stated otherwise, may include any additional additive, adjuvant or compound. Rather, the term "consisting essentially of … …" excludes any other component, step or process from the scope of any of the terms recited below, as those out of necessity for operability. The term "consisting of … …" does not include any components, steps or processes not specifically described or listed. The term "or" refers to the listed individual members or any combination thereof unless explicitly stated otherwise.
In order to make the technical problems, technical schemes and beneficial effects solved by the utility model more clear, the utility model is further described in detail below with reference to the embodiments.
Examples
The following examples are presented herein to demonstrate preferred embodiments of the present utility model. It will be appreciated by those skilled in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function in the practice of the utility model, and thus can be considered to constitute preferred modes for its practice. Those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit or scope of the utility model.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs, the disclosure of which is incorporated herein by reference as is commonly understood by reference.
Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the utility model described herein. Such equivalents are intended to be encompassed by the claims.
The experimental methods in the following examples are conventional methods unless otherwise specified. The instruments used in the following examples are laboratory conventional instruments unless otherwise specified; the test materials used in the examples described below, unless otherwise specified, were purchased from conventional biochemical reagent stores.
Example 1 cervical biological sample self-sampling device and preparation thereof
The cervical cell self-sampling device disclosed by the patent CN21744856U of the referenced Chinese utility model comprises a sleeve piece for sleeving a hand and a brush head fixedly arranged at the top end of the sleeve piece; the brush head comprises a main peak part positioned at the center of the top end of the sleeve, and a plurality of brush hairs which are arranged outside the main peak part and lower than the main peak part, as shown in fig. 1-4.
In this example, the brush head and the sleeve are both made of silica gel (US Dow Corning RBB-2003-30 silica gel) and are integrally formed by using a die. Specifically, silica gel (0.5% platinum catalyst (platinum content is 5000 ppm)) is added into a mould, then the mould is put into the mould, the temperature is 120 ℃, and the mould is removed to obtain the finished product of the cervical biological sample self-sampling device. And then the high-purity graphite is used as a carbon ion source to inject the cervical biological sample self-sampling device brush head, and the injection parameters are as follows:
energy: 10kev; ion dose: 1X 10 15 ~5×10 16 icos／cm 2 The method comprises the steps of carrying out a first treatment on the surface of the Beam current density: 0.22 mu A/cm 2 The method comprises the steps of carrying out a first treatment on the surface of the Residual pressure<3×10 -3 Pa, finally rise to 6X 10 -4 Pa. And (3) carrying out carbon ion implantation on the material by adopting a multifunctional ion implanter.
Example 2 verification of mechanical Properties of carbon-Silicone rubber
The cervical self-sampling device requires the user to sense the sampling position by himself, so the requirement on the material of the suite is very high. Thus, to further enhance the performance of cervical biological sample self-sampling devices, the inventors prepared the following cervical biological sample self-sampling devices:
(1) Cervical biological sample self-sampling device #1
And (3) placing silica gel (0.5% of platinum catalyst is added) into a die, then, heating to 120 ℃, and demoulding to obtain the cervical biological sample self-sampling device #1.
(2) Cervical biological sample self-sampling device #2
Placing silica gel (0.5% platinum catalyst) into a mold, heating to 120deg.C, demolding, and using high purity graphite as carbon ion source, and performing 1×10 ion implantation with multifunctional ion implanter 15 icos／cm 2 Carbon ion implantation to obtain cervical biological sample self-sampling device #2.
(3) Cervical biological sample self-sampling device #3
Placing silica gel (0.5% platinum catalyst) into a mold, heating to 120deg.C, demolding, and using high purity graphite as carbon ion source, and performing 3×10 ion implantation with multifunctional ion implanter 15 icos／cm 2 Carbon ion implantation to obtain cervical biological sample self-sampling device #3.
(4) Cervical biological sample self-sampling device #4
Placing silica gel (0.5% platinum catalyst) into a mold, heating to 120deg.C, demolding, and using high purity graphite as carbon ion source, and performing 6×10 ion implantation with multifunctional ion implanter 15 icos／cm 2 Carbon ion implantation to obtain cervical biological sample self-sampling device #4.
(5) Cervical biological sample self-sampling device #5
Placing silica gel (0.5% platinum catalyst) into a mold, heating to 120deg.C, demolding, and using high purity graphite as carbon ion source, and performing 1×10 ion implantation with multifunctional ion implanter 16 icos／cm 2 Carbon ion implantation, cervical biological sample self-sampling device #5 was obtained.
(6) Cervical biological sample self-sampling device #6
Placing silica gel (0.5% platinum catalyst) into a mold, heating to 120deg.C, demolding, and using high purity graphite as carbon ion source, and using multifunctional ion implanter to make the materialBy 3X 10 16 icos／cm 2 Carbon ion implantation, cervical biological sample self-sampling device #6 was obtained.
(7) Cervical biological sample self-sampling device #7
Placing silica gel (0.5% platinum catalyst) into a mold, heating to 120deg.C, demolding, and using high purity graphite as carbon ion source, and performing 5×10 ion implantation with multifunctional ion implanter 16 icos／cm 2 Carbon ion implantation to obtain cervical biological sample self-sampling device #7.
Performance tests were performed on each cervical biological sample self-sampling device prepared as described above, and the results are shown in table 1:
TABLE 1 verification of carbon-silicon rubber mechanical Capacity
| Number of self-sampling device | Shore hardness, shore A | Tensile strength, MPa | Elongation at break% | Tear Strength, kN/m | Compression set percentage,% |
| #1 | 34 | 7.2 | 628.6 | 34.8 | 0 |
| #2 | 35 | 7.1 | 629.7 | 35.3 | 0 |
| #3 | 35 | 7.2 | 629.3 | 34.9 | 0 |
| #4 | 34 | 7.2 | 628.4 | 34.5 | 1 |
| #5 | 35 | 7.1 | 630.4 | 35.2 | 0 |
| #6 | 34 | 7.2 | 633.0 | 35.7 | 2 |
| #7 | 34 | 7.2 | 621.5 | 34.6 | 1 |
As can be seen from table 1, the mechanical properties of the silica gel are not affected by carbon ion implantation.
Example 3 cell adhesion Capacity verification of cervical biological sample self-sampling device
In this example, the inventors tested the cell adhesion capability of each cervical biological sample self-sampling device prepared in example 2.
1 self-sampling device #1 to #7 for taking clean cervical biological samples are sheared to 0.5cm in an ultra-clean bench 2 Size, ultrasonic cleaning, cleaning with 75% ethanol, and drying under ultraviolet rays.
Using human cervical epithelial cells, 5mL of a cell suspension was prepared, 1mL of DNA was extracted using a Kangzhi DNA extraction kit (Universal Genomic DNA Kit, cat. CW2298M) to obtain 50. Mu.L of an eluate, and the concentration was measured with Qubit 4.0 to be 50 ng/. Mu.L, whereby 1mL of the human cervical epithelial cell suspension was found to extract 2.5. Mu.g of DNA. 0.5mL of each cell suspension was placed in 7 1.5mL centrifuge tubes.
The fragments of the cervical biological sample self-sampling device are respectively placed into different centrifuge tubes (one cervical biological sample self-sampling device corresponds to one centrifuge tube), and after standing for 5 seconds, the cell suspension is sucked by a gun head.
1mL of PBS buffer was added to each centrifuge tube, and the PBS buffer was repeatedly aspirated 3 times for DNA extraction, and the total amount of extracted DNA was as shown in Table 2 and FIG. 5:
table 2 verification of cell adhesion capability of cervical biological sample self-sampling device
| Number of self-sampling device | DNA quality (ng) |
| #1 | 303 |
| #2 | 374 |
| #3 | 466 |
| #4 | 553 |
| #5 | 574 |
| #6 | 592 |
| #7 | 621 |
As can be seen from table 2 and fig. 5, the cervical biological sample self-sampling device after carbon ion implantation has better cell adhesion capability than the cervical biological sample self-sampling device without carbon ion implantation, and the larger the carbon ion dosage, the better the effect. But the carbon ion dose increases to a certain extent (1×10) 16 icos／cm 2 ) After that, the cell adhesion capacity no longer increases significantly.
Example 4 verification of bacteriostatic Capacity of cervical biological sample self-sampling device
In this example, the inventors tested the bacteriostatic ability test of each cervical biological sample self-sampling device prepared in example 2.
1 self-sampling device #1 to #7 for taking clean cervical biological samples are sheared to 0.5cm in an ultra-clean bench 2 Size of the product. After ultrasonic cleaning, 75% ethanol cleaning and ultraviolet drying are carried out.
Preparation of CFU value of 1.5X10 by E.coli 6 0.1mL of the homogenized bacterial suspension was pipetted into 7 2.0mL centrifuge tubes, and 0.9mL of sterile medium was pipetted into each centrifuge tube.
The fragments of the cervical biological sample self-sampling device are respectively placed in different centrifuge tubes (one cervical biological sample self-sampling device corresponds to one centrifuge tube), and each centrifuge tube is placed in a 37 ℃ incubator for 12 hours.
The cervical biological sample in each centrifuge tube was removed from the collection device fragments, washed 3 times with sterile PBS, placed into a new 2mL centrifuge tube, added with 1mL sterile PBS using a sterile pipette, and shaken for 5 minutes.
Preparing beef extract peptone culture medium, pouring the beef extract peptone culture medium into a flat plate, respectively sucking 0.1mL of liquid from each centrifuge tube in an ultra-clean bench, adding the liquid into different culture dishes, uniformly smearing the bacterial liquid on the surface of the culture medium by using a coater, and rotating the culture dishes during coating to ensure that the bacterial liquid is uniformly distributed until the liquid is air-dried.
After the dishes were placed in an incubator at 37℃for 48 hours, the number of colonies was counted and the results are shown in Table 3 and FIG. 6:
table 3 verification of bacteriostatic ability of cervical biological sample self-sampling device
As can be seen from table 3 and fig. 6, the cervical biological sample self-collection device after carbon ion implantation has better antibacterial ability than the cervical biological sample self-collection device without carbon ion implantation, and the larger the carbon ion dosage during implantation, the better the antibacterial effect. But the carbon ion dose increases to a certain extent (1×10) 16 icos／cm 2 ) After that, the bacteriostatic ability is no longer significantly increased.
Example 5 cervical biological sample self-sampling device sample verification
Screening female panelists meeting the following conditions:
(1) Age: 26-65;
(2) A sexual life history;
(3) Non-menstrual colporrhagia;
the self-mining devices #1, #2, #4 and #6 were used for self-mining by randomly dividing the plants into 4 groups, and the self-mining method is as follows:
(1) The hands are cleaned, the sterilizing cotton is taken out to sterilize the periphery of the root of the finger which is taken out from the sampling device, and the sampling device is worn on the finger.
(2) The proper body position (squat type, supine type, recommended squat type) is that the self-sampling device is stretched into vagina, cervical orifice is sensed (the middle concave of circular protrusion in cavity is cervical orifice), middle peak of brush head is stretched into cervical orifice, finger is gently drawn clockwise for 10-15 weeks, after brushing is finished, the self-sampling device is placed in sample preservation solution.
Meanwhile, doctors sample and compare in a traditional way.
The sample was extracted using a general column type genomic DNA extraction kit CW2298 from Kangji corporation, and using a ZYMO DNA conversion kit (EZ DNA Methylation-Lightning) TM KIT, cat.no.: D5031) and finally methylation detection and high risk HPV16, 18 detection. The results are shown in Table 4:
TABLE 4 sampling test results
It can be seen that the concentration of the sample obtained by sampling with the cervical biological sample self-sampling device after carbon ion implantation is higher than that obtained by sampling with the cervical biological sample self-sampling device without carbon ion implantation, and the dose of the carbon ion implantation is 6×10 15 icos／cm 2 The time reaches the maximum, the obtained sample concentration is the maximum, and the detection result is the most accurate.
All documents mentioned in this disclosure are incorporated by reference in this disclosure as if each were individually incorporated by reference. Further, it will be appreciated that various changes and modifications may be made by those skilled in the art after reading the above teachings, and such equivalents are intended to fall within the scope of the utility model as defined in the appended claims.
Claims (7)
1. An application of a cervical biological sample self-collection device with antibacterial capability in preparing a kit for automatically collecting cervical biological samples by a subject in a cervical biological sample detection kit, wherein the preparation method of the cervical biological sample self-collection device comprises the following steps: heating the silica gel at 100-150 ℃ after putting the silica gel into a mold, demolding, and performing carbon ion implantation by taking graphite as a carbon ion source, wherein the carbon ion implantation dosage is 1 multiplied by 10 16 ~5×10 16 icos／cm 2 The cervical biological sample self-sampling device is obtained and comprises a sleeve piece sleeved on a hand and a brush head fixedly arranged at the top end of the sleeve piece; the brush head comprises a main peak part positioned at the center of the top end of the sleeve member and a plurality of brush hairs which are arranged outside the main peak part and lower than the main peak part.
2. Use according to claim 1, characterized in that 0.3% -0.8% of platinum catalyst is added before and/or during heating.
3. Use according to claim 2, characterized in that the platinum catalyst has a platinum content of 5000ppm.
4. The use according to claim 1, wherein the cervical biological sample self-sampling device has a thickness of 3-5 mm.
5. The use according to any one of claims 1 to 4, wherein carbon ion implantation is performed on the brush head of the cervical biological sample self-sampling device.
6. The use of claim 1, wherein the kit further comprises DNA extraction reagents.
7. The use according to claim 1 or 6, wherein the kit further comprises a methylation detection reagent and/or an HPV detection reagent.
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| CN101880402A (en) * | 2010-06-30 | 2010-11-10 | 中国人民解放军第三军医大学第二附属医院 | Surface modification method for medical grade silicon rubber |
| CN103044699A (en) * | 2011-10-11 | 2013-04-17 | 中国科学院理化技术研究所 | Method for preparing medical polymer material by ion implantation technique |
| CN105377148A (en) * | 2013-05-03 | 2016-03-02 | 费玛塞斯公司 | Methods and devices for endometrial cell and tissue sampling |
| CN217447856U (en) * | 2022-04-18 | 2022-09-20 | 昂凯生命科技(苏州)有限公司 | Cervical cell is from sampling device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101880402A (en) * | 2010-06-30 | 2010-11-10 | 中国人民解放军第三军医大学第二附属医院 | Surface modification method for medical grade silicon rubber |
| CN103044699A (en) * | 2011-10-11 | 2013-04-17 | 中国科学院理化技术研究所 | Method for preparing medical polymer material by ion implantation technique |
| CN105377148A (en) * | 2013-05-03 | 2016-03-02 | 费玛塞斯公司 | Methods and devices for endometrial cell and tissue sampling |
| CN217447856U (en) * | 2022-04-18 | 2022-09-20 | 昂凯生命科技(苏州)有限公司 | Cervical cell is from sampling device |
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