CN116273511A - Intelligent centrifuge tube for detecting centrifugal working condition in real time and using method - Google Patents

Intelligent centrifuge tube for detecting centrifugal working condition in real time and using method Download PDF

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Publication number
CN116273511A
CN116273511A CN202310081070.3A CN202310081070A CN116273511A CN 116273511 A CN116273511 A CN 116273511A CN 202310081070 A CN202310081070 A CN 202310081070A CN 116273511 A CN116273511 A CN 116273511A
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centrifugal
data
test tube
cloud platform
microprocessor
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徐昌一
桂学成
李禹增
王芸
霍钰鸣
刘雨
李文娅
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Dalian University of Technology
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Dalian University of Technology
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B13/00Control arrangements specially designed for centrifuges; Programme control of centrifuges
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers

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Abstract

The invention belongs to the fields of biology, chemistry, medical treatment, petrochemical industry, agriculture and food sanitation, and provides an intelligent centrifuge tube for detecting centrifugal working conditions in real time and a use method thereof. The intelligent test tube plug is internally provided with a laser emission module, and the intelligent test tube sleeve is internally provided with an acceleration sensor and a laser sensor. The laser sensor and the acceleration sensor send light intensity and acceleration data into the microprocessor and calculate the centrifugal degree, the rotation number and the centrifugal force; the microprocessor transmits the calculated centrifugal degree, the calculated rotation number and the calculated centrifugal force to the cloud platform through the communication chip; the cloud platform issues the data to the mobile phone APP. According to the invention, through the collection of sensor data, the microprocessor calculates the collected data, and the microprocessor, the cloud platform and the mobile phone APP communicate with each other, so that the working state of the test tube in the centrifugal machine can be mastered on the mobile phone in real time, the accurate operation of the centrifugal machine is realized, and the method has a wide application prospect.

Description

Intelligent centrifuge tube for detecting centrifugal working condition in real time and using method
Technical Field
The invention relates to the fields of biology, chemistry, medical treatment, petrochemical industry, agriculture and food sanitation, in particular to an intelligent centrifuge tube for detecting centrifugal working conditions in real time and a use method thereof.
Background
Centrifugation tasks are widely available in the fields of biology, chemistry, medical treatment, petrochemical industry, agriculture, food sanitation, and the like. For different tasks, the time required for completing the centrifugation is different, the centrifugation is usually performed by using a centrifuge to set a fixed time, the centrifugation is incomplete when the set time is too short, and the centrifugation effect may be poor and time is wasted when the set time is too long. The real-time monitoring of the centrifugal degree is a problem to be solved, and the existing device for detecting the centrifugal state in real time is an improvement of a centrifugal machine. Xiong Chenyang in "design and development of a rotational speed detection system for a medical centrifuge" (chinese medical equipment "2019,34 (04): 47-51) a method for obtaining a rotational speed by placing a hall sensor on a rotational shaft of a centrifuge is proposed, however, due to complexity of the centrifuge equipment, maintenance is difficult, and the cost is generally increased greatly for improvement of the centrifuge. In view of the above, it is necessary to develop an apparatus capable of grasping the degree of centrifugation in real time and which is simple and convenient to operate to solve the problem.
Disclosure of Invention
In order to solve the problems in the background technology, the invention aims to provide an intelligent centrifuge tube for detecting the centrifugal working condition in real time and a use method thereof, which are used for detecting the centrifugal working condition in real time, and are simple and convenient to operate.
The technical scheme adopted by the invention is as follows: an intelligent centrifuge tube for detecting the centrifugal working condition in real time comprises an intelligent test tube plug 1, a test tube 2, an intelligent test tube sleeve 3, a cloud platform 5 and a mobile phone APP6; the intelligent test tube sleeve 3 is sleeved at the tail part of the test tube 2, and the intelligent test tube plug 1 is arranged at the top of the test tube 2; the intelligent test tube plug 1 is embedded with a laser emission module 14 in the middle to emit calibrated laser; the intelligent test tube sleeve 3 is internally provided with a control device 4 for acquiring centrifugal degree, rotation number and centrifugal force data, and the acquired data is uploaded to the cloud platform 5; the cloud platform 5 issues data to the mobile phone APP6.
The control device 4 mainly comprises a fixed bracket 7 and a circuit board 8, wherein the circuit board 8 is positioned above the fixed bracket 7; the laser sensor 11 is positioned in the middle of the circuit board 8 and is used for receiving the laser emitted by the laser emitting module 14; the microprocessor 9, the communication chip 10, the laser sensor 11, the power module 12 and the acceleration sensor 13 are all positioned on the circuit board 8 and are connected with each other; the laser sensor 11 collects centrifugal degree, the acceleration sensor 13 collects rotation number and centrifugal force data, the collected data are transmitted to the microprocessor 9 for operation, and the data are uploaded to the cloud platform 5 through the communication chip 10.
The microprocessor 9, the cloud platform 5 and the mobile phone APP6 are sequentially communicated; the data node of the cloud platform 5 can store the data provided by the microprocessor 9, and the mobile phone APP6 can access the data of the cloud platform 5 according to a communication protocol. The mobile phone APP6 can communicate with the cloud platform (5) and simultaneously provides a visual interface so as to inquire centrifugal data in real time.
The microprocessor 9 receives the data of the laser sensor 11 and the acceleration sensor 13 to calculate and complete the judgment of the centrifugal state; the microprocessor 9 uploads data to the cloud platform 5 through the communication chip 10, and the cloud platform 5 issues the data to the mobile phone APP6, so that the centrifugal work can be mastered in real time.
An intelligent centrifuge tube using method for detecting the centrifugal working condition in real time is characterized in that a data node for storing information of a microprocessor 9 is arranged on a cloud platform 5, and communication between the cloud platform 5 and the microprocessor 9 and between the cloud platform and a mobile phone APP6 are completed; measuring the total weight m of the intelligent test tube plug 1 and the intelligent test tube sleeve 3 installed on the test tube 2 and the rotation radius R of the centrifugal process, and finally calibrating laser generated by the laser emitting module 14 arranged in the intelligent test tube plug 1 to obtain the light intensity values of the liquid in the test tube 2 in different states; in the centrifugation process, the laser sensor 11 positioned in the intelligent test tube sleeve 3 acquires the current light intensity and uploads the current light intensity to the microprocessor 9, and the acceleration sensor 13 positioned in the intelligent test tube sleeve 3 acquires the current acceleration and uploads the current acceleration to the microprocessor 9; the microprocessor 9 converts the data transmitted by the laser sensor 11 and the acceleration sensor 13 into data capable of reflecting the centrifugal degree, and acquires centrifugal force and rotation number information; the microprocessor 9 communicates with the cloud platform 5 through the communication chip 10, and finally transmits the data to the mobile phone APP6 to obtain the centrifugal degree, the centrifugal force and the rotation number, and compares the obtained centrifugal degree data with a preset centrifugal completion threshold value.
The acquisition of centrifugal force and rotation number information is specifically as follows:
step 1: acquiring centripetal acceleration a of the test tube through an acceleration sensor (13);
step 2: calculating the centrifugal force F by a formula ce =ma;
Step 3: calculating the angular velocity of the centrifugal process according to the centrifugal force obtained in the step 2
Figure BDA0004067430450000021
Step 4: calculating the number of rotation turns according to the angular velocity w obtained in the step 3 through a formula
Figure BDA0004067430450000022
Step 5: to calculate the centrifugal force F ce The number of rotations C is uploaded to a cloud platform (5);
step 6: the cloud platform (5) transmits the data to the mobile phone APP (6), and centrifugal force and rotation number information are displayed on the mobile phone.
The centrifugal degree is obtained as follows;
step (1): calibrating the laser generated by the laser emitting module 14 to acquire data received by the laser sensor 11 before the centrifugation starts;
step (2): the laser emitting module 14 emits laser light;
step (3): the laser sensor 11 positioned in the circuit board 8 receives the photoelectric signal of the laser emitting module 14 and transmits the photoelectric signal to the microprocessor 9;
step (4): the microprocessor 9 receives and processes the data entered by the laser sensor 11; the photoelectric signals with different sizes correspond to different centrifugal degrees, and the received photoelectric signals in the centrifugal process attenuate to less than 20% of the initial photoelectric signals to be considered to be finished in the centrifugal process; dividing the interval between 20% of the initial photoelectric signals and the initial photoelectric signals, converting the photoelectric signals into centrifugal degrees, and uploading the centrifugal degrees to the cloud platform 5;
step (5): the mobile phone APP6 receives data representing the centrifugal degree in the cloud platform 5, compares the data with a preset threshold value, and prompts the mobile phone APP6 after the centrifugation is completed.
The invention has the beneficial effects that: according to the intelligent test tube sleeve and the intelligent test tube plug, the centrifugal degree, the rotation number and the centrifugal force data are collected, and three-party communication is realized by combining the cloud platform and the mobile phone APP, so that the working state of the test tube in the centrifugal machine can be mastered on the mobile phone in real time, and the intelligent test tube sleeve and the intelligent test tube plug can be used in multiple fields of biology, chemistry, medical treatment and the like, and have a large application prospect. According to the device and the method, the centrifugal machine is not required to be improved, the device and the method are only installed on a test tube through simple external equipment, centrifugal related data are measured, three-party communication of a microprocessor, a cloud platform and a mobile phone APP is achieved, the centrifugal working state can be mastered in real time, and the device and the method have wide application potential.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of a smart test tube plug;
FIG. 3 is a schematic illustration of a smart test tube cartridge;
FIG. 4 is a schematic diagram of a control device;
FIG. 5 is a flow chart for obtaining centrifugal force, number of revolutions data;
fig. 6 is a flow chart for obtaining the degree of centrifugation.
In the figure: 1 is an intelligent test tube plug, 2 is a test tube, 3 is an intelligent test tube sleeve, 4 is a control device, 5 is a cloud platform, 6 is a mobile phone APP,7 is a fixed support, 8 is a circuit board, 9 is a microprocessor, 10 is a communication chip, 11 is a laser sensor, 12 is a power supply module, 13 is an acceleration sensor, and 14 is a laser emission module.
Detailed Description
The invention will be further described with reference to the drawings and embodiments.
As shown in fig. 1, an intelligent centrifuge tube for detecting a centrifugal operation condition in real time includes: the intelligent test tube plug 1, the test tube 2, the intelligent test tube sleeve 3, the cloud platform 5 and the mobile phone APP6; the intelligent test tube plug 1 is positioned at the top of the test tube 2, and the intelligent test tube sleeve 3 is sleeved at the bottom of the test tube 2; the cloud platform 5 is provided with data nodes for storing information, and the mobile phone APP6 can access the information stored by the data nodes in the cloud platform 5. The cloud platform 5 can communicate with the control device 4 and the mobile phone APP6 in the intelligent test tube sleeve 3. During operation, the intelligent test tube sleeve 3 can obtain centrifugal degree, rotation number and centrifugal force data, and the data are uploaded to a data node of the cloud platform 5, and the cloud platform 5 stores the data. When the mobile phone APP6 sends out a query request, the cloud platform 5 transmits the data to the mobile phone APP6, the mobile phone APP6 can display the centrifugal related data in real time, the centrifugal degree is compared with a set threshold value in the mobile phone APP6, and prompt is carried out after the centrifugation is completed. So far, the data is transmitted from the intelligent test tube sleeve 3 to the mobile phone APP6.
As shown in fig. 2, the smart test tube plug 1 is embedded with a laser emitting module 14, wherein the laser emitting module 14 is located in the smart test tube plug 1 for emitting the calibrated laser light.
As shown in fig. 3, the intelligent test tube sleeve 3 is internally provided with a control device 4, and the control device 4 is used for receiving, calculating and transmitting signals.
As shown in fig. 4, the control device 4 is composed of a fixing bracket 7 and a circuit board 8, wherein the fixing bracket 7 fixes the control device 4 in the intelligent test tube sleeve 3, so that the circuit board 8 is prevented from being shifted in position in the centrifugal process, and a hollow structure is adopted to reduce the weight of the intelligent test tube sleeve 3. The circuit board 8 is positioned right above the fixed bracket 7; the circuit board 8 consists of a microprocessor 9, a communication chip 10, a laser sensor 11, a power supply module 12 and an acceleration sensor 13; the communication chip 10 is used for communication between the microprocessor 9 and the cloud platform 5, the power supply module 12 is used for providing stable power for the whole device, the laser sensor 11 is positioned in the middle of the circuit board 8 so as to better receive laser, the microprocessor 9 is positioned on the right side of the laser sensor 11, the acceleration sensor 13 is positioned below the laser sensor 11, the power supply module 12 is positioned on the left side of the laser sensor 11, and the communication chip 10 is positioned on the left upper side of the laser sensor 11.
As shown in fig. 5, this figure shows the steps of receiving centrifugal force and rotation number information:
step 0: the tube weight m after the intelligent tube sleeve and the radius of rotation R during centrifugation are measured.
Step 1: the centripetal acceleration a of the test tube is obtained by an acceleration sensor.
Step 2: calculating the centrifugal force F by a formula ce :F ce =ma。
Step 3: from the centrifugal force obtained in step 2, the angular velocity of the centrifugal process can be calculated
Figure BDA0004067430450000051
Step 4: calculating the number of rotation turns according to the angular velocity w obtained in the step 3 through a formula
Figure BDA0004067430450000052
Step 5: to calculate the centrifugal force F ce The number of rotations C is uploaded to the cloud deck 5.
Step 6: the cloud platform 5 transmits the data to the mobile phone APP6, and centrifugal force and rotation number information are displayed on the mobile phone;
and (3) repeating the steps 1-6, so that the centrifugal related data can be checked on the mobile phone APP in real time.
As shown in fig. 6, this figure shows a real-time monitoring step of the centrifugal degree:
step (0): the laser light intensity generated by the laser emitting module 14 is calibrated to obtain the initial light intensity without shielding.
Step (1): the laser emitting module 14 generates laser light after calibration.
Step (2): a laser sensor 11 located in the circuit board 8 receives the photo-electric signal of the laser light emitted by the laser emitting module 14 and passes the data into the microprocessor 9.
Step (3): the microprocessor 9 receives the data transmitted by the laser sensor 11, processes the data, converts the data into data representing the centrifugal degree, and uploads the data to the cloud platform 5;
step (4): the mobile phone APP6 receives the data representing the centrifugal degree in the cloud platform 5, compares the data with a preset threshold value corresponding to the completion of the centrifugation, and prompts the mobile phone APP after the centrifugation is completed.
And (3) repeating the steps (1) - (4), so that the centrifugation degree can be mastered on the mobile phone APP6 in real time.
The working principle is as follows:
firstly, arranging data nodes for storing microprocessor information on a cloud platform 5, and completing communication between the cloud platform 5 and a microprocessor 9 and between the cloud platform and a mobile phone APP6; and then measuring the total weight m of the intelligent test tube plug 1 and the intelligent test tube sleeve 3 on the test tube 2 and the rotation radius R of the centrifugal process, and finally calibrating the laser generated by the laser emitting module 14 arranged in the intelligent test tube plug 1 to obtain the light intensity value of the liquid in the test tube 2 under different states. During centrifugation, the laser sensor 11 located in the intelligent test tube sleeve 3 acquires the current light intensity and uploads it to the microprocessor 9, and the acceleration sensor 13 located in the intelligent test tube sleeve 3 acquires the current acceleration and uploads it to the microprocessor 9. The microprocessor 9 processes the photoelectric signal and converts it into data reflecting the degree of centrifugation; and calculating the acceleration data by using a related formula to acquire centrifugal force and rotation number information. The microprocessor 9 communicates with the cloud platform 5 through the communication chip 10, and uploads the calculation data to the cloud platform 5, and the cloud platform 5 stores the acquired information in a preset data node. The mobile phone APP6 can acquire information stored in the data node, namely the centrifugal degree, the centrifugal force and the rotation number according to the corresponding communication protocol, compares acquired centrifugal degree data with a preset centrifugal completion threshold value, and prompts the mobile phone APP6 after the centrifugal completion.

Claims (7)

1. The intelligent centrifuge tube for detecting the centrifugal working condition in real time is characterized by comprising an intelligent test tube plug (1), a test tube (2), an intelligent test tube sleeve (3), a cloud platform (5) and a mobile phone APP (6); the intelligent test tube sleeve (3) is sleeved at the tail part of the test tube (2), and the intelligent test tube plug (1) is arranged at the top of the test tube (2); a laser emitting module (14) is embedded in the center of the intelligent test tube plug (1) to emit calibrated laser; the intelligent test tube sleeve (3) is internally provided with a control device (4) which is used for collecting centrifugal degree, rotation number and centrifugal force data, and the collected data is uploaded to the cloud platform (5); and the cloud platform (5) transmits the data to the mobile phone APP (6).
2. The intelligent centrifuge tube for detecting the centrifugal working condition in real time according to claim 1, wherein the control device (4) mainly comprises a fixed bracket (7) and a circuit board (8), and the circuit board (8) is positioned above the fixed bracket (7); the laser sensor (11) is positioned in the middle of the circuit board (8) and is used for receiving laser emitted by the laser emitting module (14); the microprocessor (9), the communication chip (10), the laser sensor (11), the power module (12) and the acceleration sensor (13) are all positioned on the circuit board (8) and are connected with each other; the laser sensor (11) collects centrifugal degree, the acceleration sensor (13) collects rotation number and centrifugal force data, the collected data are transmitted to the microprocessor (9) for operation, and the data are sent to the cloud platform (5) through the communication chip (10).
3. The intelligent centrifuge tube for detecting the centrifugal working condition in real time according to claim 2, wherein the microprocessor (9), the cloud platform (5) and the mobile phone APP (6) are communicated in sequence; the data node of the cloud platform (5) can store data provided by the microprocessor (9), and the mobile phone APP (6) can access the data of the cloud platform (5) according to a communication protocol.
4. The intelligent centrifuge tube for detecting the centrifugal working condition in real time according to claim 2, wherein the microprocessor (9) receives the data of the laser sensor (11) and the acceleration sensor (13) for calculation, and the judgment of the centrifugal state is completed; the microprocessor (9) uploads data to the cloud platform (5) through the communication chip (10), and the cloud platform (5) issues the data to the mobile phone APP (6), so that the centrifugal work can be mastered in real time.
5. An intelligent centrifuge tube using method for detecting centrifugal working conditions in real time is characterized in that data nodes for storing information of a microprocessor (9) are arranged on a cloud platform (5), and communication between the cloud platform (5) and the microprocessor (9) and communication between the cloud platform and a mobile phone APP (6) are completed; measuring the total weight m of the intelligent test tube plug (1) and the intelligent test tube sleeve (3) installed on the test tube (2) and the rotation radius R in the centrifugal process, and finally calibrating laser generated by a laser emitting module (14) arranged in the intelligent test tube plug (1) to obtain the light intensity values of liquid in the test tube (2) in different states; in the centrifugal process, a laser sensor (11) positioned in the intelligent test tube sleeve (3) acquires current light intensity and uploads the current light intensity to the microprocessor (9), and an acceleration sensor (13) positioned in the intelligent test tube sleeve (3) acquires current acceleration and uploads the current acceleration to the microprocessor (9); the microprocessor (9) converts data transmitted by the laser sensor (11) and the acceleration sensor (13) into data capable of reflecting the centrifugal degree and acquires centrifugal force and rotation number information; the microprocessor (9) communicates with the cloud platform (5) through the communication chip (10), and finally transmits the data to the mobile phone APP (6) to obtain the centrifugal degree, the centrifugal force and the rotation number, and compares the obtained centrifugal degree data with a preset centrifugal completion threshold value.
6. The method for using the intelligent centrifugal tube for detecting the centrifugal working condition in real time according to claim 5, wherein,
the acquisition of centrifugal force and rotation number information is specifically as follows:
step 1: acquiring centripetal acceleration a of the test tube through an acceleration sensor (13);
step 2: calculating the centrifugal force F by a formula ce =ma;
Step 3: calculating the angular velocity of the centrifugal process according to the centrifugal force obtained in the step 2
Figure FDA0004067430440000021
Step 4: calculating the number of rotation turns according to the angular velocity w obtained in the step 3 through a formula
Figure FDA0004067430440000022
Step 5: to calculate the centrifugal force F ce The number of rotations C is uploaded to a cloud platform (5);
step 6: the cloud platform (5) transmits the data to the mobile phone APP (6), and centrifugal force and rotation number information are displayed on the mobile phone.
7. The method for using the intelligent centrifugal tube for detecting the centrifugal working condition in real time according to claim 5, wherein the centrifugal degree is obtained as follows;
step (1): calibrating laser generated by a laser emitting module (14) to acquire data received by a laser sensor (11) before centrifugation starts;
step (2): a laser emitting module (14) emits laser light;
step (3): a laser sensor (11) positioned in the circuit board (8) receives the photoelectric signal of the laser emitting module (14) and transmits the photoelectric signal to the microprocessor (9);
step (4): a microprocessor (9) receives and processes the data transmitted by the laser sensor (11); the photoelectric signals with different sizes correspond to different centrifugal degrees, and the received photoelectric signals in the centrifugal process attenuate to less than 20% of the initial photoelectric signals to be considered to be finished in the centrifugal process; dividing the interval between 20% of the initial photoelectric signals and the initial photoelectric signals, converting the photoelectric signals into centrifugal degrees, and uploading the centrifugal degrees to a cloud platform (5);
step (5): the mobile phone APP (6) receives data representing the centrifugal degree in the cloud platform (5), compares the data with a preset threshold value, and prompts the mobile phone APP (6) after the centrifugation is completed.
CN202310081070.3A 2023-02-08 2023-02-08 Intelligent centrifuge tube for detecting centrifugal working condition in real time and using method Pending CN116273511A (en)

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