CN116256191A - Sheeting roller for tissue slice electrostatic collection system and tissue slice electrostatic collection system - Google Patents
Sheeting roller for tissue slice electrostatic collection system and tissue slice electrostatic collection system Download PDFInfo
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- CN116256191A CN116256191A CN202310131083.7A CN202310131083A CN116256191A CN 116256191 A CN116256191 A CN 116256191A CN 202310131083 A CN202310131083 A CN 202310131083A CN 116256191 A CN116256191 A CN 116256191A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65H—HANDLING THIN OR FILAMENTARY MATERIAL, e.g. SHEETS, WEBS, CABLES
- B65H18/00—Winding webs
- B65H18/02—Supporting web roll
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65H—HANDLING THIN OR FILAMENTARY MATERIAL, e.g. SHEETS, WEBS, CABLES
- B65H18/00—Winding webs
- B65H18/08—Web-winding mechanisms
- B65H18/10—Mechanisms in which power is applied to web-roll spindle
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
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Abstract
The invention relates to a sheeting roller for a tissue slice electrostatic collection system and the tissue slice electrostatic collection system, wherein the electrostatic collection system comprises a cutter, a polymer film conveying belt for electrostatically adsorbing tissue slices and conveying the tissue slices, and a sheeting roller for controlling the distance between the polymer film conveying belt and the upper surface of the cutter: the sheeting roller comprises a roller body for supporting a high polymer film conveying belt, wherein the high polymer film conveying belt penetrates through the upper surface of the cutter and between the roller bodies along the conveying direction of the high polymer film conveying belt, when the radius of the sheeting roller meets the conditions defined by the invention, the high polymer film conveying belt has good electrostatic adsorption effect on biological slices, and meanwhile, the samples can be prevented from colliding with the sheeting roller before cutting and from being adhered and extruded during collection of the sample slices.
Description
Technical Field
The invention belongs to the field of biomedicine, and particularly relates to a sheeting roller for a tissue slice electrostatic collection system and the tissue slice electrostatic collection system.
Background
In the biomedical field, biological tissues are often processed into slices so as to be suitable for microscopic observation, and the biological tissue processing method has wide application in biological imaging and pathological research. In addition, there is also a need in the art for whole organ imaging based on mechanical ablation to slice tissue. In large scale or systematic studies, these flakes need to be collected. In the related art, slice collection is performed by manual operation, because slices are extremely thin and fragile, curling, wrinkling, cracking and similar damages can occur, and there are also automatic collection modes of tissue slices, such as a rolling-to-rolling-up mode based on adhesive tapes, which are matched with a rolling-up roller and an unrolling roller, and combined with the adhesive property of the adhesive tapes, sample collection is completed by adopting a mode of synchronously rolling up while cutting, and some schemes use special conductive plastic belts, and the slice collection is realized by utilizing electrostatic adsorption force, but when biological tissue slices are collected, adhesion extrusion is relatively easy to occur in the collection process due to the thinner thickness of the tissue slices, so that the surface of the tissue slices is subjected to extrusion deformation, and the thickness of the collected tissue slices is uneven, so that the tissue slice collection effect is greatly influenced.
Disclosure of Invention
The technical problems solved by the invention are as follows: the invention provides a sheeting roller for a tissue slice electrostatic collection system and the tissue slice electrostatic collection system, when the radius of the sheeting roller meets the conditions defined by the invention, the charged high polymer film conveying belt has good electrostatic adsorption effect on biological slices, and can prevent samples from colliding with the sheeting roller before cutting and preventing the samples from adhering and extruding.
The technical scheme based on the invention is as follows:
the present invention provides a sheeting roller for a tissue slice electrostatic collection system, the electrostatic collection system comprising: the device comprises a cutter, a high polymer film conveying belt for electrostatically adsorbing and conveying tissue slices, and a sheeting roller for controlling the distance between the high polymer film conveying belt and the upper surface of the cutter; the sheeting roller comprises a roller body for supporting a polymer film conveying belt, the distance L between the upper surface of a cutter and the outer periphery of the roller body is more than or equal to h, the distance di between the tip of the cutter and the outer periphery of the roller body is in the range of h-3 h, the polymer film conveying belt penetrates through the upper surface of the cutter and between the roller bodies along the conveying direction of the polymer film conveying belt, and the radius of the roller body meets the following conditions:
wherein R is the radius of the roller body;
h is the thickness of the tissue slice;
h is the thickness of the high polymer film transmission belt;
θ is the angle between the upper surface of the tool and the cutting plane.
Based on the technical scheme of the invention, the method has the following beneficial effects:
the sheeting roller is used for controlling the distance between the high polymer film conveying belt and the cutter and the sample, so that good collection of slices is realized, the cutter is used for cutting biological tissue samples, when the radius of the sheeting roller meets the conditions, the charged high polymer film conveying belt has good electrostatic adsorption effect on biological slices, and meanwhile, the samples can be effectively prevented from colliding with the sheeting roller before cutting and the samples are prevented from being adhered and extruded during collection.
Based on the scheme, the invention can also be improved as follows:
further, the sheeting roller comprises a roller body, an assembly part and a supporting piece used for supporting the polymer film conveying belt, the roller body is rotatably installed on the assembly part, the supporting piece is fixed on the assembly part and is close to the upper surface of the cutter, the roller body and the supporting piece are sequentially arranged along the conveying direction of the polymer film conveying belt, and the polymer film conveying belt penetrates through the upper surface of the cutter and between the supporting pieces.
Further, the supporting piece is of a plate-shaped structure, and the contact part of the supporting piece and the high polymer film conveying belt is of a curved surface structure.
Further, the support member and the assembly member are integrally formed
Further, a viewing window is provided on the support.
The invention also provides a tissue slice electrostatic collection system, comprising: the high polymer film conveying belt is used for electrostatically adsorbing the tissue slices and conveying the tissue slices, and the sheeting roller for electrostatically collecting the tissue slices is used for supporting the roller body of the high polymer film conveying belt, the distance L between the upper surface of the cutter and the outer periphery of the roller body is more than or equal to H, the distance di between the cutter tip of the cutter and the outer periphery of the roller body is in the range of (H+h) - (H+3h), and the high polymer film conveying belt is arranged between the upper surface of the cutter and the roller body in a penetrating mode along the conveying direction of the high polymer film conveying belt.
Further, the device is used for imaging the sample slice adsorbed on the polymer film conveying belt, and the cutter and the slice imaging device are sequentially arranged along the conveying direction of the polymer film conveying belt.
Further, the device is used for transferring the tissue slice onto a glass slide for observation imaging, the cutter and the slice transferring device are sequentially arranged along the conveying direction of the high polymer film conveying belt, and the slice transferring device is selected from one of a mechanical arm transferring device, a temperature difference transferring device, a UV glue transferring device, a liquid drop eliminating static electricity transferring device and a low-viscosity adhesive tape transferring device.
Further, still include unreeling device and coiling mechanism, unreel device includes first unreel roller and magnetic powder brake, the coiling mechanism includes first wind-up roll and step motor, first unreel roller electricity is connected the magnetic powder brake, first wind-up roll electricity is connected step motor, polymer film conveyer belt both ends are around locating respectively on first unreel roller and the first wind-up roll.
Further, the device also comprises a plurality of steering rollers for controlling the trend of the whole polymer film conveying belt loop, wherein each steering roller is arranged between the first unreeling roller and the first reeling roller.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
Fig. 1 is a schematic diagram of the positional relationship between a sheeting roller and a cutter, and between the sheeting roller and a sample according to an embodiment of the present invention.
Fig. 2 is a schematic diagram of the positional relationship between the sheeting roller and the cutter, and between the sheeting roller and the sample in the case of collision between the sample and the sheeting roller.
FIG. 3 is a graph of roll radius, tip distance from the outer periphery of the roll, and tissue slice thickness.
FIG. 4 is a graph of roll radius, angle between the upper surface of the tool and the cutting plane, and tissue slice thickness.
Fig. 5 is a schematic diagram of the positional relationship of a sheeting roller and a cutter in accordance with one embodiment of the present invention.
Fig. 6 is a schematic view of the structure of a sheeting roller in accordance with one embodiment of the present invention.
Fig. 7 is a schematic view showing the positional relationship between a sheeting roller and a cutter according to another embodiment of the present invention.
Fig. 8 is a schematic structural view of a tissue slice electrostatic collection system in accordance with an embodiment of the present invention.
Fig. 9 is a schematic perspective view of a sterilization cell, a charging device, and an electrostatic measurement instrument in a tissue slice electrostatic collection system in accordance with one embodiment of the present invention.
Fig. 10 is a schematic diagram of the structure of a high voltage charging device in a tissue slice electrostatic collection system in accordance with an embodiment of the present invention.
Fig. 11 is a schematic perspective view of a sterilization cell, a charging device, and an electrostatic measuring instrument in a tissue slice electrostatic collection system in accordance with another embodiment of the present invention.
Fig. 12 is a schematic structural view of a triboelectric charging device in a tissue slice electrostatic collection system, in accordance with an embodiment of the present invention.
In fig. 1-12, the parts are represented by the following names:
1. a polymer film transfer belt;
2. a charging device; 21. a high pressure generating head; 22. a ground electrode; 23. a first translation stage; 24. a second translation stage; 25. friction electrification rod;
3. a steering roller;
4. an electrostatic measuring instrument;
5. a sterilizing pool;
6. a sheeting roller; 61. a roller body; 62. a fitting; 63. a support; 631. a guide roller;
7. a cutter; 71. a sample; 72. tissue sections;
8. an observation window;
9. a slice imaging device;
10. a first unreeling roller;
11. and a first wind-up roll.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
As shown in fig. 1, a sheeting roller for a tissue slice electrostatic collection system according to the present invention, the electrostatic collection system comprising: a cutter 7, a polymer film transfer belt 1 for electrostatically adsorbing and transferring a tissue slice, and a sheeting roller for controlling a distance between the polymer film transfer belt 1 and an upper surface of the cutter 7; the sheeting roller 6 comprises a roller body 61 for supporting a polymer film conveying belt, the polymer film conveying belt is arranged between the upper surface of the cutter 7 and the roller body 61 in a penetrating manner along the conveying direction, and the radius of the roller body 61 meets the following conditions:
(R+H+h+dtandθ)cosθ≥R+H+h(I)
(di+R+H) 2 ＝(R+H+h) 2 +d 2 (II)
h<di-H≤3h(III)
wherein R is the radius of the roller body;
di is the length obtained by subtracting the radius of the roller body from the distance between the cutter point and the central axis of the roller body, and is defined as the distance between the cutter point and the outer periphery of the roller body;
d is the distance between the cutter point and the tabletting;
h is the thickness of the tissue slice;
h is the thickness of the high polymer film transmission belt;
di-H is the distance between the cutter point and the adsorption surface of the polymer film conveying belt;
θ is the angle between the upper surface of the tool and the cutting plane.
The roller 61 is used for controlling the distance between the high polymer film conveyer belt 1 and the cutter 7 (or the sample 71) to realize good collection of slices, the cutter is used for cutting the sample 71 of biological tissues to form tissue slices, when the radius R of the tabletting roller meets the above conditions, the charged high polymer film conveyer belt has good electrostatic adsorption effect on the biological slices, and meanwhile, the collision of the sample with the tabletting roller (shown in fig. 2) before cutting and the adhesion extrusion of the sample slices are prevented.
Simplifying the formula (I) to obtain:
as can be seen from formula (II), di increases with increasing d when
When di takes the minimum value, namely:
as can be seen from the formula (IV), the distance di between the tip and the outer periphery of the roller body is as follows:
at this time, the roller body is pressed on the knife surface (namely, the roller body, the polymer film conveying belt, the sample slice and the knife surface on the knife are sequentially pressed and contacted).
When collecting biological tissue slices, because the thickness of the tissue slices is thinner, the adhesion extrusion is easy to occur in the slice automatic collection technology based on roll-to-roll, the extrusion deformation is caused on the surfaces of the tissue slices, the thickness of the collected tissue slices is uneven, and the tissue slice collection effect is greatly influenced. The inventors found the following phenomena according to actual film collecting experience: the di parameter and the film collecting effect are most directly related, and the parameter is important because in the actual film collecting process, the sections with the size of tens of micrometers are found to be very easy to roll, particularly, the front end of the tissue section is continuously rolled at the cutter point along with the cutting, until the thickness of the rolled section (with the continuous rolling of the front end, the thickness is continuously increased, the thickness of the rolled section becomes 2H, and the thickness of the rolled section becomes 3H) is equivalent to the (di-H) value (namely, the distance between the cutter point and the adsorption surface of the polymer film conveying belt), and the tissue section can be close to or contacted with the polymer film conveying belt, so that the tissue section is collected by electrostatic adsorption of the polymer film conveying belt, and therefore, the control of the di-H is extremely important. In our practical film collection experience, it was found that the distance between the tip and the suction surface of the polymeric film transport belt (i.e., di-H) was as follows: when H is less than or equal to 3H, the tissue sample is not easy to be extruded and stuck, and under the optimal condition, the di-H is controlled between H and 2H, so that the tissue sample has a good collecting effect. In addition, according to the above formula, if di-H is controlled within the above range, the radius R of the roll body and the installation angle theta of the cutter are preferably selected, and in addition, the optimal tabletting position of the roll body can be calculated when different roll body radii R and cutter installation angles theta are selected, so that a certain basis is provided for adjusting the tabletting rolls.
Specifically, it is assumed that the thickness H of the polymer film transfer belt is 0 (i.e., in the case where the polymer film thickness H is negligible):
when the included angle between the upper surface of the cutter and the horizontal plane is θ=45°, the relationship between the cutter tip and the roll body distance di, the roll body radius R and the tissue slice thickness h is shown in fig. 8. In this case, if the distance di-H between the blade edge and the suction surface of the polymer film transfer belt is desirably controlled to be H < di-H.ltoreq.2h, the roll radius should be selected so as not to exceed 11H. For example, when the slice thickness h=50 μm, the different roll radii R are selected as (h=0):
when it is desired to control h < di.ltoreq.2h, the maximum radius of the roll body is 0.56mm.
When it is desired to control 2h < di < 3h, the maximum radius of the roll body is 1.16mm.
When it is desired to control 3h < di < 4h, the maximum radius of the roll body is 1.77mm.
When the distance di-H between the cutter point and the adsorption surface of the polymer film conveyer belt is 2H (h=0), the relationship between the maximum radius R of the required roller body and the included angle (i.e. cutter angle) between the upper surface of the cutter and the cutting plane is:
at this time, as shown in fig. 9, a graph of the relationship among the roll radius R, the cutter angle (θ), and the tissue slice thickness H, when the slice thickness h=50 μm, the different roll radius R is selected as (h=0):
when the cutter angle is 10 degrees, the maximum radius of the roller body is 13.00mm.
When the cutter angle is 20 degrees, the maximum radius of the roller body is 3.20mm.
When the cutter angle is 30 degrees, the maximum radius of the roller body is 1.35mm.
When the cutter angle is 40 degrees, the maximum radius of the roller body is 0.70mm.
The above analysis is directed to preventing the front end of a tissue slice from curling adhesion and collecting the tissue slice by electrostatic adsorption under a specific cutter angle and slice thickness, for example, the maximum radius of the roll body is 13.00mm when the cutter angle is 10 ° at slice thickness h=50um.
Specifically, when designing the roll body radius, the roll body radius range is determined according to the required slice tissue thickness and the cutter angle, and a specific roll body radius is selected in the range. When the tissue slice collecting system is designed, the distance L between the upper surface of the cutter 7 and the outer periphery of the roller body is firstly determined, the distance di between the cutter point of the cutter and the outer periphery of the roller body is determined, and electrostatic adsorption collection is carried out under the condition. Specifically, when l=h+h, the roller body performs tabletting on the blade surface (i.e., the roller body, the polymer film conveying belt, the sample slice, and the blade surface on the cutter are sequentially pressed and contacted), and when the electrostatic tabletting is performed, the roller body does not necessarily have to perform tabletting on the blade surface (e.g., the distance L > h+h between the upper surface of the cutter and the outer periphery of the roller body) and is adsorbed on the polymer film conveying belt under the action of electrostatic force.
Preferably, the included angle θ between the upper surface of the tool and the cutting plane (i.e., the mounting angle of the tool) is in the range of 30 ° < θ <50 °. Under the condition, a higher roller radius is obtained, and a better electrostatic sheet collecting effect is achieved. The larger the radius of the roller body is, the smoother the transition is when the conveying belt turns, the more the contact area and the contact time with the sample are, the certain improvement on the sheet collecting effect is realized, but in actual use, the mounting angle of the cutter is limited to a certain extent, namely, cutter manufacturers usually make the cutter angle (the included angle between the cutter upper cutter surface and the cutter back) about 30 degrees, under the condition that the installation of props is feasible, the mounting angle is reduced as much as possible, the roller body radius is improved, and when the mounting angle range of the cutter based on the invention is between 30 and 50 degrees, the larger roller body radius can be obtained, and the requirements of electrostatic absorption sample slicing and sample slice transportation are met.
As shown in fig. 5 and 6, according to the embodiment of the present invention, the sheeting roller 6 includes a roller body 61, an assembly 62 and a support member 63 for supporting the polymer film conveyer belt, the roller body 61 is rotatably mounted on the assembly 62, the support member is fixed on the assembly and is disposed near the upper surface of the cutter, the roller body 61 and the support member are sequentially disposed along the conveying direction of the polymer film conveyer belt, and the polymer film conveyer belt 1 is disposed between the upper surface of the cutter 7 and the support member 63. For the film collecting mode based on electrostatic adsorption, the magnitude of electrostatic adsorption force is inversely proportional to the square of the distance, so that the distance between a charged polymer film conveying belt and a tissue slice needs to be reduced as much as possible so as to facilitate electrostatic adsorption of the tissue slice, and the arrangement of a supporting piece can prolong the time of close-range action between the polymer film conveying belt and the tissue slice (because the tissue slice possibly moves a distance along the surface of a cutter after the slice is cut, if the arrangement of the supporting piece is not provided, the polymer film conveying belt immediately changes the direction after passing through a roller body and does not leave enough close-range electrostatic adsorption time for the charged polymer film conveying belt, so that collection of the tissue slice is difficult to complete through electrostatic adsorption); based on the calculation, we can know that for the tissue slice with the thickness of the micron level, the preferred radius of the roller body is only a few millimeters, even the micron level, therefore, the short-distance electrostatic adsorption time of the charged high polymer film transmission belt on the surface of the roller body and the tissue slice is very short, the short-distance electrostatic action time between the high polymer film transmission belt and the tissue slice can be obviously improved by arranging the supporting piece, the high polymer film transmission belt is accurately and effectively matched with the cutter, and the collection effect of the tissue slice is improved.
According to the sheeting roller for the electrostatic tissue slice collecting system of the embodiment of the present invention, the supporting member 63 has a plate-shaped structure, and the contact part between the supporting member and the polymer film conveyor belt 1 is configured as a curved surface structure.
Preferably, as shown in fig. 6 and 7, according to the embodiment of the present invention, the support 63 is integrally formed with the assembly 62, and we vertically extend the assembly 62 of the lamination roller to a part, so as to reduce the distance between the subsequent polymer film conveyer belt and the sample, prolong the short-distance electrostatic action time between the charged polymer film conveyer belt and the tissue slice 72 on the cutter, and reduce the assembly work of the lamination roller.
Alternatively, as shown in fig. 5, the supporting member includes a plate body 632 and a guide roller 631 for supporting the polymer film transport belt, and the guide roller 631 is rotatably installed on the plate body 632. Besides adopting a curved surface structure at the contact position, the guide roller can be selected as a supporting position of the high polymer film conveying belt in the supporting piece, and the short-distance acting time between the high polymer film conveying belt and the tissue slice 72 is improved through the cooperation of the guide roller and the roller body in the tabletting roller.
As shown in fig. 6, the support 63 is provided with a viewing window 8 for viewing the adhesion state of the slices, according to the embodiment of the invention. The observation window 8 facilitates observation of the adhesion effect of the tissue slice, and facilitates parameter debugging in the early stage and observation of the subsequent electrostatic film collecting effect.
As shown in fig. 8, according to the electrostatic tissue slice collecting system of the present invention, the cutter 7, the polymer film transfer belt for electrostatically adsorbing and transporting the tissue slices, and the sheeting roller 6 for electrostatic tissue slice collection as described above, the sheeting roller 6 includes a roller body 61 for supporting the polymer film transfer belt 1, the distance L between the upper surface of the cutter 7 and the outer periphery of the roller body is equal to or greater than h, the distance di between the tip of the cutter 7 and the outer periphery of the roller body is in the range of h to 3h, and the polymer film transfer belt 1 is disposed between the upper surface of the cutter 7 and the roller body 61 in the transporting direction thereof.
Specifically, before tissue slice is collected, the positions of the cutter and the roller body are fixed, so that the distance from the tangent line of the high polymer transmission belt contacted with the roller body to the upper surface of the cutter is constant, the distance di between the cutter tip of the cutter 7 and the outer periphery of the roller body is constant, when tissue slice collection is required, a sample moves to the conveying direction of the high polymer film belt, the tissue slice is formed by cutting by the cutter, and meanwhile, the tissue slice is collected by electrostatic adsorption by the high polymer film transmission belt moving in the same direction as the sample.
Preferably, during cutting, the movement speed of the polymer film conveying belt is the same as that of the sample, so that the tissue slice is ensured not to be misplaced and deformed after being adsorbed on the polymer film conveying belt.
As shown in fig. 8, the electrostatic collection system for tissue slices according to the embodiment of the invention further comprises a slice imaging device 9 for imaging a sample slice adsorbed on the polymer film conveyor belt, wherein the cutter and the slice imaging device are sequentially arranged along the conveying direction of the polymer film conveyor belt 1.
The tissue slice electrostatic collection system based on the embodiment of the invention further comprises a slice transfer device, wherein the slice transfer device is used for transferring the tissue slice onto a glass slide for observation imaging, the cutter and the slice transfer device are sequentially arranged along the conveying direction of the high polymer film conveying belt 1, and the slice transfer device is selected from one of a mechanical arm transfer device, a temperature difference transfer device, a UV adhesive transfer device, a liquid drop eliminating electrostatic transfer device and a low-viscosity adhesive tape transfer device. Tissue slices are transferred onto a slide using existing slice transfer equipment and then imaged for viewing. For example, for transfer of frozen sections, robotic arm transfer devices, low tack tape direct transfer, temperature differential transfer, and UV glue transfer devices may be used; the transfer of paraffin sections may use a robotic transfer device, a low tack tape, and a drop-out transfer device. Tissue slices are transferred onto a slide using existing slice transfer equipment and then imaged for viewing.
According to the tissue slice electrostatic collection system provided by the embodiment of the invention, the sample is an embedded block embedded with a living body sample. In the prior art, the process of preparing tissue slices by embedding blocks is generally as follows: the living sample is embedded with an embedding medium and then cut into slices having an extremely thin thickness of several micrometers by an existing cutter or other cutting means, and then further the slices are taken out and transported to a subsequent extension step (such as imaging observation).
Specifically, the high polymer film transmission belt for electrostatically adsorbing and conveying the tissue slices is obtained by electrifying the high polymer film transmission belt through a high-voltage induction and friction electrification technology. The electrostatic generation technology (high-voltage charge, friction electrification, separation charge and the like) is combined with the stable high-molecular film transmission belt to be applied to the field of biological tissue slice collection by utilizing the property of electrostatic energy capable of absorbing light and small objects, so that tissue slices are collected and transported; the high polymer film conveying belt is convenient to convey the biological tissue slices for secondary transfer, has small damage and less pollution to the tissue slices, is not easy to adhere tissue residues through the high polymer film conveying belt for adsorbing samples by electrostatic acting force, and is convenient for recycling.
Preferably, the electrostatic collection system for tissue slices according to the embodiment of the invention adopts a charging device 2 to charge the polymer film transmission belt. The charging device comprises a high-voltage charging device, as shown in fig. 9 and 10, the high-voltage charging device comprises a high-voltage generating head 21 and a grounding electrode 22 which is arranged opposite to the high-voltage generating head, the high-polymer film conveyer belt is arranged between the high-voltage generating head 21 and the grounding electrode 22 in a penetrating way along the conveying direction of the high-polymer film conveyer belt, and the charging device further comprises a first translation stage 23 for adjusting the distance between the high-voltage generating head 21 and the high-polymer film conveyer belt and a second translation stage 24 for adjusting the distance between the grounding electrode 22 and the high-polymer film conveyer belt; or the charging device is a friction charging device, as shown in fig. 11 and 12, the friction charging device includes a plurality of friction charging bars 25, and each friction charging bar 25 is sequentially arranged along the conveying direction of the polymer film conveying belt.
Optionally, the charging device is a split charging device. The separation charge means that when the contacted objects are separated, such as tearing off the adhesive tape or removing the protective film on the LCD glass substrate, a strong charging phenomenon occurs, the more closely the two are contacted, the higher the density of the charge is, the discharge is caused, the amount of the charge is determined by the separation speed, and when the separation speed is high, the charge is increased.
Preferably, the high voltage charging device is an electrostatic high voltage generator. The high voltage generating head of the electrostatic high voltage generator is used for generating high voltage of tens kilovolts, the copper block with good conductivity is used as the grounding electrode, and is opposite to the high voltage electrode, so that a strong electric field is generated between the high voltage generating head and the grounding electrode, wherein the distance between the high polymer film transmission belt and the high voltage generating head, the distance between the high polymer film transmission belt and the grounding electrode, the moving speed of the high polymer film transmission belt and the material of the high polymer film transmission belt can influence the charging effect of the high polymer film transmission belt, the high voltage generating head and the grounding electrode are respectively held by two translation stages, and the precise control of the distance between the high polymer film transmission belt and the high voltage generating head and the distance between the high polymer film transmission belt and the grounding electrode can be realized through the precise adjustment of the translation stages. The friction electrification bars in the friction electrification device are used for generating friction with the to-be-electrified high polymer film conveying belt, so that the high polymer film conveying belt is electrified, the control of the electrification quantity of the high polymer film conveying belt is realized by adjusting the arrangement and the quantity of the friction electrification bar arrays, and the electrification quantity of the high polymer film conveying belt can be influenced by surrounding environmental conditions such as temperature and humidity and mechanical factors such as contact, friction, rotation and twisting, friction force, friction direction, friction speed, contact area, contact time and the like. Because of the limitations of static research and the complexity of the materials of the conveying belt, no related method is available at present for realizing a proper and stable charging method of the conveying belt, and by combining two main static generation modes, the uniform and stable charge on the conveying belt of the polymer film can be realized through the adjustment of the conveying belt of the polymer film, a charging device and charging conditions (such as the movement of a translation stage, the speed, the temperature, the humidity and the number and the arrangement of friction charging rods of the conveying belt of the polymer film). Specifically, the method can determine the charging parameters suitable for automatically collecting the tissue slices according to the polymer film transmission belts made of different materials and the slices with different thicknesses, and then the polymer film transmission belts are used for collecting the tissue slices after the polymer film transmission belts are charged with stable static charges, so that the automatic collection of the biological tissue slices is completed.
According to the tissue slice electrostatic collection system provided by the embodiment of the invention, the high polymer film conveying belt is a high polymer transparent film conveying belt, and the high polymer transparent film conveying belt material is selected from one of polyethylene, polypropylene, polystyrene, polytetrafluoroethylene, polyester or polyvinyl chloride. The polymer film material has high resistivity, high strength (because the loop has large tension when being wound, the film should generate as little deformation as possible), transparency, stable charge and stable transport of tissue slices, convenient observation and no pollution to samples.
As shown in fig. 8, the tissue slice electrostatic collection system according to the embodiment of the invention further comprises an unreeling device and a reeling device, wherein the unreeling device comprises a first unreeling roller 10 and a magnetic powder brake, the reeling device comprises a first reeling roller 11 and a stepping motor, the first unreeling roller 10 is electrically connected with the magnetic powder brake, the first reeling roller 11 is electrically connected with the stepping motor, and two ends of the polymer film conveyer belt 1 are respectively wound on the first unreeling roller 10 and the first reeling roller 11. The polymer film conveyer belt discharged from the first unreeling roller 10 is charged with static electricity and then enters between a cutter and a tabletting roller (a sample 71 is cut into tissue slices by the cutter), under the cooperation of the static electricity effect and the cutter and the tabletting roller, the collection and the transportation of the tissue slices are realized, and finally the polymer film conveyer belt is reeled by the first reeling roller.
Specifically, the first unreeling roller 10 is used for clamping a transmission belt roll positioned at the unreeling end of the polymer film transmission belt, the first unreeling roller 10 is connected with a magnetic powder brake, the tension of the whole polymer film transmission belt loop can be controlled by controlling the input current of the magnetic powder brake, the first reeling roller 32 is used for clamping the transmission belt roll positioned at the reeling end of the polymer film transmission belt, the first reeling roller 32 is connected with a direct current stepping motor, and the rotating speed of the stepping motor is controlled by controlling the pulse frequency of the input pulse motor, so that the advancing speed of the whole polymer film transmission belt is controlled. In order to realize automatic collection of tissue slices by utilizing static electricity, a proper amount of stable static electricity is required to be charged on a high polymer film transmission belt, and related theoretical researches on static electricity are very limited, at present, the static electricity generation process has no unified and clear theoretical guidance, the static electricity generation process also involves complex interactions with materials, and for different materials and static electricity generation parameters, the static electricity generation effect has obvious differences.
As shown in fig. 8, preferably, in the tissue slice collecting system according to an embodiment of the present invention, the charging device 2, the roller body 61 and the slice imaging device 9 are disposed between the first unreeling roller 10 and the first reeling roller 11, and the charging device 2, the sheeting roller 6 and the slice imaging device 9 are disposed in this order along the conveying direction of the polymer film conveyer. During operation, the positions of the cutter and the roller body are fixed, the first unreeling roller 10 and the first winding roller 11 are started to enable the polymer film conveying belt to move at a speed v, after the polymer film conveying belt enters the charging device 2 to carry static electricity, the sample 71 is moved at the speed v along the conveying direction of the polymer film conveying belt 1 to start slicing, the polymer film conveying belt 1 which is wound on the roller body 61 and is in a moving state absorbs the cut tissue slices 72 and drives the tissue slices to continuously move along the conveying direction under the action of static electricity and under the cooperation of the cutter 7 and the roller body 61 (shown in fig. 7), the collection and conveying of the polymer film conveying belt 1 to the tissue slices 72 are achieved, then the polymer film conveying belt is conveyed to the position where the slice imaging device is located to carry out subsequent direct imaging or conveyed to the position where the slice transferring device is located to carry out tissue slice transferring, and finally the first winding roller 11 rolls the polymer film conveying belt.
As shown in fig. 9, the tissue slice electrostatic collection system according to an embodiment of the present invention further includes an electrostatic measuring device 4 for measuring an electrostatic voltage of the polymer film transport belt 1, and the charging device 2 and the electrostatic measuring device 4 are sequentially disposed along a transport direction of the polymer film transport belt 1. The electrostatic measuring instrument is used for measuring the voltage of the high polymer film transmission belt, and further optimizes and adjusts each charge parameter through the measurement value of the electrostatic measuring instrument, specifically, for a high-voltage charge mode, when the measurement value of the electrostatic measuring instrument is lower than the charge amount required by electrostatic film collection, the generation voltage of the electrostatic high-voltage generator can be increased, the running speed of the motor can be reduced, the distance between the electrostatic generation head and the high polymer film transmission belt can be reduced, or the distance between the high polymer film transmission belt and the grounding electrode can be reduced; for the friction charging mode, the friction force between the high polymer film transmission belt and the friction charging rods can be increased by reducing the input current of the magnetic powder brake, the number of the friction charging rods is increased, and the electric charge quantity is increased by changing the rotating speed of the stepping motor. In actual use, the charging parameters required by the high-voltage charging device and the friction charging device can be determined according to the actual collecting effect and the measurement result of the electrostatic measuring instrument when the tissue slice is successfully collected under a certain thickness.
Preferably, according to the tissue slice electrostatic collection system of one embodiment of the present invention, the thickness of the tissue slice is 10 μm to 50 μm, and the charged voltage of the polymer transparent film is 5kV to 8kV when measured with an electrostatic measuring instrument at a distance of 5mm from the polymer transparent film. Under the condition, stable and continuous collection of tissue slices can be realized.
The electrostatic collection system for tissue slices is combined, tissue slices with the thickness of 30um are taken as samples, a polyethylene transparent film belt is taken as a high polymer film transmission belt, the electrostatic measurement instrument is used for determining that the high polymer transparent film can achieve that the required charge voltage for collecting the tissue slices is 6kv at the position 5mm away from the high polymer transparent film transmission belt, then a high-voltage charging device and a friction charging device are respectively used as charging devices, and several parameters with larger influence on the charge effect of the high polymer film transmission belt are determined and adjusted, so that the measurement of the charge quantity of the high polymer film transmission belt and the adjustment of the charge quantity form closed-loop control, and the specific charge parameters for collecting the tissue slices with the thickness of 30um can be achieved as shown in the following table 1.
TABLE 1 tissue section collection experience parameters for 30um thickness
The electrostatic collection system for tissue slices according to an embodiment of the invention, as shown in fig. 9, further comprises a plurality of turning rolls 3 for controlling the direction of the whole polymer film conveying belt loop, wherein each turning roll 3 is arranged between the first unreeling roll and the first reeling roll.
As shown in fig. 9, the electrostatic collection system for tissue slices according to an embodiment of the present invention further comprises a sterilization tank 5, wherein the sterilization tank and the charging device 2 are sequentially arranged along the conveying direction of the polymer film conveyer belt. Because the conveyor belt is required to be used for collecting tissue slices of biological samples, the surface of the conveyor belt needs to be ensured to be clean so as not to cause unnecessary pollution to the samples, different reagents can be added into a sterilizing tank according to different cleanliness requirements of the tissue slices, and the sterilization treatment of the high polymer film conveyor belt can be completed before electrostatic charging.
Specifically, the tissue slice collection system further comprises a frame to which the sheeting rollers 6 are mounted by the fitting 61.
Preferably, the frame is adjustable in position of the sheeting rollers. Specifically, the frame includes an offset adjustment device that is connected to the fitting 62 in the sheeting roller to adjust the spatial attitude of the roller body in four degrees of freedom, so that the distance from the tangent line of the polymeric film transport belt in contact with the roller body to the upper surface of the cutter is constant. The offset adjusting device can be used for adjusting the tabletting roller or the compression bar in the existing tissue slice collecting system, and is connected with the tabletting roller to adjust the space posture of the roller body in four degrees of freedom, so that the offset of the tabletting roller in the four degrees of freedom is eliminated, and the distance from the tangent line of the contact between the high polymer film conveying belt and the roller body of the tabletting roller to the upper surface of the cutter is constant.
Although embodiments of the present invention have been described in detail above, one of ordinary skill in the art will appreciate that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the invention, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. A sheeting roller for a tissue slice electrostatic collection system, the electrostatic collection system comprising: a cutter, a polymer film conveying belt for electrostatically adsorbing and conveying the tissue slices, and a sheeting roller (6) for controlling the distance between the polymer film conveying belt and the upper surface of the cutter; the sheeting roller (6) comprises a roller body (61) for supporting a polymer film conveying belt, the polymer film conveying belt penetrates through the upper surface of the cutter and between the roller bodies (61) along the conveying direction of the polymer film conveying belt, and the radius of the roller body (61) meets the following conditions:
wherein R is the radius of the roller body;
h is the thickness of the tissue slice;
h is the thickness of the high polymer film transmission belt;
θ is the angle between the upper surface of the tool and the cutting plane.
2. The sheeting roller for a tissue slice electrostatic collection system according to claim 1, wherein the sheeting roller (6) comprises a roller body (61), an assembly member (62) and a support member (63) for supporting a polymer film conveying belt, the roller body (61) is rotatably mounted on the assembly member (62), the support member is fixed on the assembly member and is arranged close to the upper surface of the cutter, the roller body (61) and the support member are sequentially arranged along the conveying direction of the polymer film conveying belt, and the polymer film conveying belt is arranged between the upper surface of the cutter and the support member (63).
3. The sheeting roller for tissue slice electrostatic collection systems as claimed in claim 2, wherein the support (63) is of a plate-like configuration and the contact of the support with the polymeric film transport belt is provided in a curved configuration.
4. The sheeting roller for tissue slice electrostatic collection systems of claim 2, wherein the support (63) is integrally formed with the fitting (62).
5. Sheeting roller for tissue slice electrostatic collection systems according to any one of claims 2-4, characterized in that the support (63) is provided with a viewing window (8).
6. A tissue slice electrostatic collection system, comprising: the utility model provides a cutter (7), be used for static absorption tissue section and transport tissue section's polymer film conveyer belt (1) and be used for tissue section static collection's preforming roller (6) according to any one of claims 1-5, preforming roller (6) are including being used for supporting polymer film conveyer belt (1) roll body (61), cutter (7) upper surface with the distance L of roll body (61) peripheral edge is not less than H, the range of cutter point of cutter (7) and roll body (61) peripheral edge's distance di is (H+h) - (H+3h), polymer film conveyer belt (1) wears to locate along its direction of transport cutter (7) upper surface and between roll body (61).
7. The electrostatic collection system according to claim 6, further comprising a slice imaging device (9) for imaging a sample slice adsorbed on the polymer film transport belt (1), the cutter and the slice imaging device being disposed in order along a transport direction of the polymer film transport belt (1).
8. The electrostatic tissue slice collecting system according to claim 6, further comprising a slice transfer device for transferring the tissue slice onto a slide for observation imaging, the cutter and the slice transfer device being disposed in order along a conveying direction of the polymer film conveyor belt (1), the slice transfer device being selected from one of a mechanical arm transfer device, a temperature difference transfer device, a UV glue transfer device, a droplet eliminating electrostatic transfer device, or a low-viscosity adhesive tape transfer device.
9. The tissue slice electrostatic collection system according to any one of claims 6-8, further comprising an unreeling device and a reeling device, wherein the unreeling device comprises a first unreeling roller (10) and a magnetic powder brake, the reeling device comprises a first reeling roller (11) and a stepping motor, the first unreeling roller (10) is electrically connected with the magnetic powder brake, the first reeling roller (11) is electrically connected with the stepping motor, and two ends of the polymer film conveyer belt (1) are respectively wound on the first unreeling roller (10) and the first reeling roller (11).
10. The tissue slice electrostatic collection system of claim 9, further comprising a plurality of turning rolls (3) for controlling the run of the entire polymeric film transfer tape loop, each turning roll (3) being disposed between the first unwind roll (10) and the first wind-up roll (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310131083.7A CN116256191A (en) | 2023-02-14 | 2023-02-14 | Sheeting roller for tissue slice electrostatic collection system and tissue slice electrostatic collection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310131083.7A CN116256191A (en) | 2023-02-14 | 2023-02-14 | Sheeting roller for tissue slice electrostatic collection system and tissue slice electrostatic collection system |
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| Publication Number | Publication Date |
|---|---|
| CN116256191A true CN116256191A (en) | 2023-06-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310131083.7A Pending CN116256191A (en) | 2023-02-14 | 2023-02-14 | Sheeting roller for tissue slice electrostatic collection system and tissue slice electrostatic collection system |
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| Country | Link |
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| CN (1) | CN116256191A (en) |
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2023
- 2023-02-14 CN CN202310131083.7A patent/CN116256191A/en active Pending
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