CN116200309A - Glutamic acid mycoprotein extraction and purification process - Google Patents
Glutamic acid mycoprotein extraction and purification process Download PDFInfo
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- CN116200309A CN116200309A CN202310190560.7A CN202310190560A CN116200309A CN 116200309 A CN116200309 A CN 116200309A CN 202310190560 A CN202310190560 A CN 202310190560A CN 116200309 A CN116200309 A CN 116200309A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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Abstract
The invention belongs to the technical field of biology, and discloses an extraction and purification process of glutamic acid mycoprotein, which comprises the following steps: heating the glutamic acid fermentation waste liquid through an evaporator; then injecting the mixture into a plate-frame filter through a pump, and collecting solid matters and plate-frame filter pressing clear liquid; adding flocculant into the plate frame filter pressing clear liquid for flocculation treatment to obtain flocculation liquid; pumping the flocculate liquid into a high-speed disc centrifuge for centrifugation to perform solid-liquid separation, and collecting precipitate; mixing the solid and the precipitate, transferring into a blast drying oven, drying to constant weight to obtain a crude mycoprotein product, and pulverizing the crude mycoprotein product by a pulverizer to obtain powdery mycoprotein. The process improves the protein yield and realizes energy-saving and high-efficiency extraction of the glutamic acid mycoprotein.
Description
Technical Field
The invention relates to the technical field of biology, in particular to an extraction and purification process of glutamic acid mycoprotein.
Background
The mycoprotein is a byproduct in the amino acid production process, is a cell protein prepared by separating and drying amino acid produced by amino acid producing bacteria, and is found to contain various nutrients such as abundant proteins, nucleic acids, saccharides, vitamins and the like by measuring the composition of the amino acid mycoprotein. Most of the existing extraction and purification processes have the defects of low extraction efficiency, high process cost and the like.
CN114805475a discloses a method for secondary extraction of protein from amino acid extraction mother liquor, the method for secondary extraction of protein from amino acid extraction mother liquor comprises: preprocessing the glutamic acid extraction mother liquor to obtain preprocessed glutamic acid extraction mother liquor; extracting pretreated glutamic acid extraction mother liquor by a protein air floatation-precipitation method to obtain crude thallus protein precipitate and supernatant; and carrying out filter pressing, centrifugation, drying and vacuum concentration treatment on the crude thallus protein precipitate and supernatant to obtain a product protein, a fertilizer stock solution and concentrated distilled water. The invention improves the protein extraction yield of the glutamic acid extraction mother liquor by 2.7-3.2%, reduces the protein residue of the filtered liquid after protein extraction from 4.7-5.2% to below 2%, and increases the concentration multiple of the fertilizer stock solution obtained by vacuum evaporation concentration from 2.1-2.3 to 2.7.
CN111018069a discloses a method for recovering microbial thallus proteins, specifically, collecting waste water of microbial proteins to be treated; separating protein scum from the microbial thallus egg wastewater; removing free water in the protein scum, and carrying out fluidization treatment on the protein scum after removing the free water; drying the fluidized protein slag to obtain the finished product protein powder.
CN110526469a discloses a process and system for treating fermentation wastewater and recovering mycoprotein, which comprises the following steps: setting a horizontal decanter centrifuge for pre-treatment of fermentation wastewater, setting a disk centrifuge for secondary concentration treatment of clear liquid after horizontal decanter centrifugation, and setting a centrifugal clear liquid tank, a feeding buffer tank, a concentrate tank, a fluidized bed dryer unit and an emulsion pump; conveying distilled fermentation wastewater to a feeding buffer tank; adding a flocculant into the centrifugal clear liquid tank; the horizontal decanter centrifuge and the disk centrifuge perform secondary separation on the centrifugal concentrate; the concentrated solution separated by the disc centrifuge enters the concentrated solution tank, and the clear solution is conveyed to a sewage treatment system, and the fermentation mash treatment process can separate most of mycoprotein through three-stage separation.
CN107418897a discloses a process for effectively utilizing glutamic acid to ferment waste mycoprotein, which comprises the following steps: step 1) centrifugation, drying and crushing, step 2) wall breaking, hydrolysis and centrifugation, and step 3) adsorption, microfiltration, ultrafiltration and drying.
However, the prior art still lacks a more cost-effective way of extraction and purification of mycoproteins.
Disclosure of Invention
In order to overcome the technical problems in the prior art, the invention provides an extraction and purification process of glutamic acid mycoprotein.
The invention is realized by the following technical scheme.
The extraction and purification process of the glutamic acid mycoprotein comprises the following steps:
heating the glutamic acid fermentation waste liquid to 85-90 ℃ by an evaporator, and preserving heat for 30-40 minutes; then injecting the mixture into a plate-frame filter by a pump, maintaining the pressure of the plate-frame at 0.3-0.5MPa, and collecting solid matters and plate-frame filter pressing clear liquid; adding flocculant into the plate frame filter pressing clear liquid for flocculation treatment to obtain flocculation liquid; pumping the flocculate liquid into a high-speed disc centrifuge for centrifugation to perform solid-liquid separation, and collecting precipitate; mixing the solid and the precipitate, transferring into a blast drying oven, drying to constant weight to obtain a crude mycoprotein product, and pulverizing the crude mycoprotein product by a pulverizer to obtain powdery mycoprotein.
Preferably, the method comprises the steps of,
the flocculant is polyacrylamide, polyaluminum chloride or a mixture of the polyacrylamide and the polyaluminum chloride.
Preferably, the method comprises the steps of,
the flocculation time is 30-60min.
Preferably, the method comprises the steps of,
the centrifugation speed was 6000rpm and the centrifugation time was 10min.
Preferably, the method comprises the steps of,
the drying temperature is 60-80 ℃.
Preferably, the method comprises the steps of,
the addition amount of the flocculant is 1-10g/L.
More preferably, the process is carried out,
the flocculant is a mixture of polyacrylamide and polyaluminium chloride according to the equal mass ratio.
The beneficial effects achieved by the invention mainly include, but are not limited to, the following aspects:
in the prior art, a flocculating agent is often added at the front extraction stage, a large amount of water is required to be added for dissolution, and the addition amount of the flocculating agent is larger; after extracting the mycoprotein, evaporating and concentrating the protein extraction tail liquid, and adding water in the flocculant intangibly increases the steam consumption, which is not beneficial to energy conservation and emission reduction. And the extracted mother liquor still contains more than 10 percent of protein residues, so that the viscosity of the mother liquor after the protein extraction is higher, and the subsequent low-temperature negative pressure concentration of the mother liquor is greatly influenced. The invention adopts steam heating to denature and separate out protein, then extracts the protein through a plate-and-frame filter press, and then flocculates the plate-and-frame filter press clear liquid through a small amount of composite flocculant, and then separates and extracts the mycoprotein for the second time through a disc separator, thereby improving the protein yield and realizing energy-saving and high-efficiency extraction of glutamic acid mycoprotein. The invention adopts the composite flocculant, and can obviously reduce the use amount of the flocculant.
Description of the embodiments
The invention is further described below, the embodiments presented in this description are only exemplary and do not limit the scope of the invention. It will be understood by those skilled in the art that the details and forms of the invention may be modified or substituted without departing from the spirit and scope of the invention.
Examples
The extraction and purification process of the glutamic acid mycoprotein comprises the following steps:
heating the glutamic acid fermentation waste liquid to 85 ℃ through an evaporator, and preserving heat for 40 minutes; then the mixture is injected into a plate-frame filter by a pump, the pressure of the plate-frame is maintained at 0.5MPa, and solid matters and plate-frame filter pressing clear liquid are collected; adding a flocculating agent polyacrylamide into the plate-frame filter pressing clear liquid, wherein the concentration is 5g/L, and the flocculation time is controlled to be 30min to obtain flocculation liquid; pumping the flocculate liquid into a high-speed disc centrifuge for solid-liquid separation, wherein the centrifugation speed is 6000rpm, the centrifugation time is 10min, and collecting precipitate; mixing the solid and the precipitate, transferring into a blast drying oven, baking at 60 ℃ to constant weight to obtain a crude mycoprotein product, and crushing the obtained mycoprotein product by a crusher to obtain powdery mycoprotein.
Through detection, the main components of the mycoprotein product are as follows: the water content is 4.1%, the crude protein content is 82.7%, and the crude ash content is 4.4%; the extraction rate of the mycoprotein is 91.4 percent.
Examples
The extraction and purification process of the glutamic acid mycoprotein comprises the following steps:
heating the glutamic acid fermentation waste liquid to 90 ℃ through an evaporator, and preserving heat for 30 minutes; then the mixture is injected into a plate-frame filter by a pump, the pressure of the plate-frame is maintained at 0.5MPa, and solid matters and plate-frame filter pressing clear liquid are collected; adding flocculating agent polyaluminium chloride with the concentration of 5g/L into the plate frame filter pressing clear liquid, and controlling the flocculation time to be 30min to obtain flocculation liquid; pumping the flocculate liquid into a high-speed disc centrifuge for solid-liquid separation, wherein the centrifugation speed is 6000rpm, the centrifugation time is 10min, and collecting precipitate; mixing the solid and the precipitate, transferring into a blast drying oven, baking at 60 ℃ to constant weight to obtain a crude mycoprotein product, and crushing the obtained mycoprotein product by a crusher to obtain powdery mycoprotein.
Through detection, the main components of the mycoprotein product are as follows: the water content is 4.2%, the crude protein content is 83.1%, and the crude ash content is 3.9%; the extraction rate of the mycoprotein is 90.7%.
Examples
The extraction and purification process of the glutamic acid mycoprotein comprises the following steps:
heating the glutamic acid fermentation waste liquid to 88 ℃ through an evaporator, and preserving heat for 35 minutes; then the mixture is injected into a plate-frame filter by a pump, the pressure of the plate-frame is maintained at 0.5MPa, and solid matters and plate-frame filter pressing clear liquid are collected; adding a composite flocculant into the plate-frame filter pressing clear liquid, wherein the concentration is 2g/L, and the flocculation time is controlled to be 30min to obtain flocculation liquid; pumping the flocculate liquid into a high-speed disc centrifuge for solid-liquid separation, wherein the centrifugation speed is 6000rpm, the centrifugation time is 10min, and collecting precipitate; mixing the solid and the precipitate, transferring into a blast drying oven, baking at 60 ℃ to constant weight to obtain a crude mycoprotein product, and crushing the obtained mycoprotein product by a crusher to obtain powdery mycoprotein.
The composite flocculant is prepared by mixing polyacrylamide and polyaluminium chloride according to a mass ratio of 1:1.
Through detection, the main components of the mycoprotein product are as follows: the water content is 3.9%, the crude protein is 84.7%, and the crude ash content is 3.6%; the extraction rate of the mycoprotein is 89.5%.
Examples
The extraction and purification process of the glutamic acid mycoprotein comprises the following steps:
heating the glutamic acid fermentation waste liquid to 88 ℃ through an evaporator, and preserving heat for 35 minutes; then the mixture is injected into a plate-frame filter by a pump, the pressure of the plate-frame is maintained at 0.5MPa, and solid matters and plate-frame filter pressing clear liquid are collected; adding a composite flocculant into the plate-frame filter pressing clear liquid, wherein the concentration is 4g/L, and the flocculation time is controlled to be 30min to obtain flocculation liquid; pumping the flocculate liquid into a high-speed disc centrifuge for solid-liquid separation, wherein the centrifugation speed is 6000rpm, the centrifugation time is 10min, and collecting precipitate; mixing the solid and the precipitate, transferring into a blast drying oven, baking at 60 ℃ to constant weight to obtain a crude mycoprotein product, and crushing the obtained mycoprotein product by a crusher to obtain powdery mycoprotein.
The composite flocculant is prepared by mixing polyacrylamide and polyaluminium chloride according to a mass ratio of 1:1.
Through detection, the main components of the mycoprotein product are as follows: the water content is 3.8%, the crude protein content is 86.3%, and the crude ash content is 3.5%; the extraction rate of the mycoprotein is 92.5%.
While the invention has been described with reference to specific embodiments thereof, it will be understood by those skilled in the art that the invention is not limited thereto, and that various changes and modifications may be made without departing from the spirit of the invention.
Claims (7)
1. The extraction and purification process of the glutamic acid mycoprotein comprises the following steps:
heating the glutamic acid fermentation waste liquid to 85-90 ℃ by an evaporator, and preserving heat for 30-40 minutes; then injecting the mixture into a plate-frame filter by a pump, maintaining the pressure of the plate-frame at 0.3-0.5MPa, and collecting solid matters and plate-frame filter pressing clear liquid; adding flocculant into the plate frame filter pressing clear liquid for flocculation treatment to obtain flocculation liquid; pumping the flocculate liquid into a high-speed disc centrifuge for centrifugation to perform solid-liquid separation, and collecting precipitate; mixing the solid and the precipitate, transferring into a blast drying oven, drying to constant weight to obtain a crude mycoprotein product, and pulverizing the crude mycoprotein product by a pulverizer to obtain powdery mycoprotein.
2. The process of claim 1, wherein the flocculant is polyacrylamide, polyaluminum chloride or a mixture of both.
3. The process according to claim 1, characterized in that the flocculation time is 30-60min.
4. The process of claim 1, wherein the centrifugation speed is 6000rpm and the centrifugation time is 10 minutes.
5. The process of claim 1, wherein the drying temperature is 60-80 ℃.
6. The process according to claim 1, wherein the flocculant is added in an amount of 1-10g/L.
7. The process according to claim 1, wherein the flocculant is a mixture of polyacrylamide and polyaluminum chloride in equal mass ratio.
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CN202310190560.7A CN116200309A (en) | 2023-03-02 | 2023-03-02 | Glutamic acid mycoprotein extraction and purification process |
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CN202310190560.7A CN116200309A (en) | 2023-03-02 | 2023-03-02 | Glutamic acid mycoprotein extraction and purification process |
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