CN116183829A - Cosmetic raw material moisturizing effect testing method and application thereof - Google Patents
Cosmetic raw material moisturizing effect testing method and application thereof Download PDFInfo
- Publication number
- CN116183829A CN116183829A CN202310166615.0A CN202310166615A CN116183829A CN 116183829 A CN116183829 A CN 116183829A CN 202310166615 A CN202310166615 A CN 202310166615A CN 116183829 A CN116183829 A CN 116183829A
- Authority
- CN
- China
- Prior art keywords
- cosmetic raw
- raw material
- moisturizing effect
- testing
- moisturizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002537 cosmetic Substances 0.000 title claims abstract description 113
- 239000002994 raw material Substances 0.000 title claims abstract description 88
- 238000012360 testing method Methods 0.000 title claims abstract description 87
- 230000003020 moisturizing effect Effects 0.000 title claims abstract description 77
- 238000001514 detection method Methods 0.000 claims abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 38
- 239000003906 humectant Substances 0.000 claims abstract description 35
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 239000007864 aqueous solution Substances 0.000 claims abstract description 15
- 238000011156 evaluation Methods 0.000 claims abstract description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 63
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 claims description 28
- 235000011187 glycerol Nutrition 0.000 claims description 24
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 claims description 22
- 229940035437 1,3-propanediol Drugs 0.000 claims description 22
- 229920000166 polytrimethylene carbonate Polymers 0.000 claims description 22
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000001540 sodium lactate Substances 0.000 claims description 5
- 229940005581 sodium lactate Drugs 0.000 claims description 5
- 235000011088 sodium lactate Nutrition 0.000 claims description 5
- 238000010998 test method Methods 0.000 claims description 4
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 2
- QWGRWMMWNDWRQN-UHFFFAOYSA-N 2-methylpropane-1,3-diol Chemical compound OCC(C)CO QWGRWMMWNDWRQN-UHFFFAOYSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 229940100573 methylpropanediol Drugs 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000000600 sorbitol Substances 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 abstract description 3
- 210000003491 skin Anatomy 0.000 description 38
- 230000000052 comparative effect Effects 0.000 description 13
- 210000000245 forearm Anatomy 0.000 description 10
- 210000001550 testis Anatomy 0.000 description 8
- 241001474374 Blennius Species 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000003814 drug Substances 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 238000005070 sampling Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- -1 Dimethylsiloxane Chemical class 0.000 description 4
- 229920002385 Sodium hyaluronate Polymers 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000001815 facial effect Effects 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000007781 pre-processing Methods 0.000 description 4
- 229940010747 sodium hyaluronate Drugs 0.000 description 4
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 229940099259 vaseline Drugs 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 241000252212 Danio rerio Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000004299 exfoliation Methods 0.000 description 1
- 235000020710 ginseng extract Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000036559 skin health Effects 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
- A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis
- A61B5/443—Evaluating skin constituents, e.g. elastin, melanin, water
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0003—Composite materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Dermatology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a method for testing the moisturizing effect of a cosmetic raw material and application thereof, wherein the method for testing the moisturizing effect of the cosmetic raw material comprises the following steps: (1) The skin moisture content and/or the percutaneous moisture loss before and after the test subjects of the control group and the test subjects are smeared with the detection reagent are respectively detected, wherein the detection reagent smeared by the test subjects of the control group is a humectant aqueous solution, and the detection reagent of the test group is an equal-concentration humectant aqueous solution containing cosmetic raw materials; (2) Calculating moisture retention evaluation index B of cosmetic raw material, and using skin moisture content increase range B 1 And/or the amplitude of the decrease in percutaneous moisture loss B 2 To evaluate the moisturizing effect of the cosmetics. The detection method provided by the invention has the advantages of real result, short time and high efficiency, and can also effectively reduce the test error of the moisturizing effect caused by the fact that the raw materials of cosmetics are volatile and have poor solubility.
Description
Technical Field
The invention belongs to the field of cosmetic raw material detection, and particularly relates to a method for testing moisturizing effect of a cosmetic raw material and application thereof.
Background
Moisturizing is the most basic requirement for skin care, and is also an important condition for ensuring skin health and delaying aging. The moisture content of the skin is directly related to the softness and elasticity of the skin, when the air temperature is reduced, the skin cuticle can not timely adjust enough moisturizing factors, the activity of oil glands is reduced, the oil and moisture on the face are reduced, the skin is tightly stretched, and the effects of moisturizing the skin, preventing skin from evaporating, improving microcirculation and enhancing skin wettability can be achieved by using the skin care moisturizing product with corresponding effects.
Moisture retention is also a very important fundamental efficacy of cosmetics, wherein the detection method of moisture retention of cosmetic raw materials includes an in vitro efficacy evaluation method and a human efficacy evaluation method. The in vitro efficacy evaluation mainly relates to moisture absorption rate, water retention rate and moisture absorption stability, and the human efficacy evaluation mainly relates to skin moisture content and distribution thereof, skin percutaneous moisture loss (TEWL) and stratum corneum exfoliation index.
CN111999291a discloses a cosmetic moisture content detection device and detection method, through with the cosmetic storage jar transport to the detection bench, and be close to sampling test paper, the drive the elevating component drives the sampling tube is close to the cosmetic storage jar in vertical direction, screw rod driving motor drive rotatory screw rod rotates, rotatory screw rod drives the supporting shoe slides on the horizontal mounting bracket, and then drives the sampling tube transversely is close to the cosmetic storage jar, and enters into the storage jar inside, the pneumatic cylinder drives the piston rod stretches out and draws back, thereby inhales the cosmetic solution through atmospheric pressure the inside of sampling tube, and transfer to detect on the sampling test paper, so can automize and carry out cosmetic moisture content detection, need not manual operation, saved the manpower.
CN112147140a discloses a method for evaluating moisturizing efficacy of cosmetics, comprising the following steps: (1) establishing a sample group; (2) establishing a blank control group; (3) establishing a micro-seepage water-shortage model group; (4) culturing at constant temperature; (5) And photographing under an dissecting microscope, storing pictures, calculating the area of the tail of each group of zebra fish, and calculating the moisturizing efficacy of the cosmetics to be tested according to a formula. The invention utilizes zebra fish model organisms to evaluate the efficacy of cosmetics, has short experimental period, low cost, transparent embryo and juvenile fish, can directly observe the change of various tissues and organs under a microscope, and has visual and easily understood experimental results.
The conventional cosmetic raw material human body moisturizing efficacy testing method in the prior art has long detection period (usually 28 days), is complex to operate, and cannot be widely applied to various cosmetic raw materials, such as volatile or insoluble cosmetic raw materials. Therefore, it is very significant to explore a moisturizing detection method which is easy to operate, short in period and widely applicable to various cosmetic raw materials.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method for testing the moisturizing effect of cosmetic raw materials and application thereof.
To achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a method for testing the moisturizing effect of a cosmetic raw material, the method comprising the steps of:
(1) The skin moisture content and/or the percutaneous moisture loss before and after the application of the detection reagent are respectively detected for the two groups of subjects in the control group and the experimental group, and the result is recorded as A Control group-front 、A Control group-post 、A Experimental group-front And A Experimental group-post ；
Wherein the detection reagent smeared by the experimental subject of the control group is a humectant aqueous solution, and the detection reagent smeared by the experimental subject of the experimental group is an equal-concentration humectant aqueous solution containing cosmetic raw materials;
(2) Calculating a moisturizing evaluation index B of the raw materials of the cosmetics,
In the method for testing the moisturizing effect of the cosmetic raw materials, the step of preprocessing the cosmetic raw materials by adding the humectant is creatively carried out in a human body moisturizing evaluation experiment, the moisturizing effect of the humectant is counteracted by using a control group only containing the humectant, and finally the moisturizing effect of the cosmetic raw materials is reflected by using the difference value between the experimental group and the control group. When the testing method provided by the invention is used for detecting the cosmetic raw materials which are not easy to volatilize and dissolve, the result is not obviously different from the conventional moisturizing effect test-28-day human body moisturizing effect test result, and the testing method provided by the invention is short in time and high in efficiency. In addition, the moisturizing agent is used for preprocessing the cosmetic raw materials, and the moisturizing agent can reduce the loss of the volatile cosmetic raw materials and the cosmetic raw materials with low solubility, and effectively reduce the testing error of the moisturizing effect caused by the fact that the cosmetic raw materials are volatile and have poor solubility.
Preferably, the humectant in the step (1) includes any one or a combination of at least two of 1, 3-propanediol, glycerol, butanediol, methyl propanediol, lactic acid, sodium lactate, mannose, sorbitol or trehalose, and the combination of at least two of the two includes a combination of 1, 3-propanediol and glycerol, a combination of glycerol and butanediol, or a combination of lactic acid and sodium lactate, etc., and any other combination modes may be selected, and will not be described in detail herein.
Preferably, the humectant of step (1) comprises 1, 3-propanediol.
When the humectant specifically comprises 1, 3-propylene glycol, the test result of the method for testing the moisturizing effect of the cosmetic raw material is close to the 28-day human body moisturizing experimental result, and the accuracy is higher.
Preferably, the humectant of step (1) further comprises glycerin.
When the humectant specifically comprises the combination of 1, 3-propylene glycol and glycerin, the test result of the method for testing the moisturizing effect of the cosmetic raw material is closer to the experimental result of 28 days of human body moisturizing when the humectant only contains 1, 3-propylene glycol, and the accuracy is further improved
Preferably, the mass ratio of the 1, 3-propanediol to the glycerol is (5-20): 1.
The specific values in (5-20) may be selected from 5, 7, 9, 11, 13, 15, 17, 19 or 20, etc.; other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the mass fraction of the humectant in the humectant aqueous solution in the step (1) is 1-10%.
Specific values in the 1-10% range may be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%, etc.; other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the mass fraction of the cosmetic raw material in the aqueous solution of the constant-concentration humectant containing the cosmetic raw material in the step (1) is 0.01-10%.
Specific values in the 0.01-10% can be selected from 0.01%, 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%, etc.; other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the detection of skin moisture content and/or transdermal moisture loss is performed within 2-10 hours after the application of the detection reagent of step (1).
Specific values in the 2-10h can be selected from 2h, 4h, 6h, 8h, 10h and the like; other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the cosmetic raw material includes a solid cosmetic raw material, a liquid cosmetic raw material, or a gaseous cosmetic raw material.
In a second aspect, the present invention provides the use of the method for testing the moisturizing effect of a cosmetic raw material according to the first aspect in evaluating the moisturizing effect of a cosmetic.
Compared with the prior art, the invention has the following beneficial effects:
in the method for testing the moisturizing effect of the cosmetic raw materials, the step of preprocessing the cosmetic raw materials by adding the humectant is creatively carried out in a human body moisturizing evaluation experiment, the moisturizing effect of the humectant is counteracted by using a control group only containing the humectant, and finally the moisturizing effect of the cosmetic raw materials is reflected by using the difference value between the experimental group and the control group. When the testing method provided by the invention is used for detecting the cosmetic raw materials which are not easy to volatilize and dissolve, the result is not obviously different from the conventional moisturizing effect test-28-day human body moisturizing effect test result, and the testing method provided by the invention is short in time and high in efficiency. In addition, the moisturizing agent is used for preprocessing the cosmetic raw materials, and the moisturizing agent can reduce the loss of the volatile cosmetic raw materials and the cosmetic raw materials with low solubility, and effectively reduce the testing error of the moisturizing effect caused by the fact that the cosmetic raw materials are volatile and have poor solubility.
Detailed Description
The technical scheme of the invention is further described by the following specific embodiments. It will be apparent to those skilled in the art that the examples are merely to aid in understanding the invention and are not to be construed as a specific limitation thereof.
The procedures, conditions, reagents, experimental methods, etc. for carrying out the present invention are common knowledge and common knowledge in the art, except for those specifically mentioned below, and the present invention is not particularly limited. The experimental methods in each example, in which specific conditions are not noted, are generally performed under conventional conditions or under conditions recommended by the manufacturer.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. However, in case of conflict, the present specification, including definitions, will control.
The seaweed extract is commercially available from Shaanxi Kangli Biotechnology Co., ltd., model number KLC-146; the ginseng extract is a commercial product available from the company of biosciences, all-round, western medicine, with the number wf-01. Dimethylsiloxane is commercially available from Union Carbide under the trade name Volatile Silicone FZ 3109. The sodium hyaluronate is a commodity with 30 ten thousand types of cosmetic grade molecular weight purchased from Shenzhen Hengsheng biotechnology power suppliers; vaseline is a commodity purchased from Henan Kai Biotechnology Co.
Example 1
The embodiment provides a method for testing the moisturizing effect of cosmetic raw materials, which comprises the following steps:
(1) A control group and an experimental group were set, each group of 10 persons (the age was 18-45 years old, the male and female were half, and any cosmetic, external medicine or oral health care product could not be used 3 days before the test of the tested part).
(2) And (3) smearing 1mL of detection reagent on the tested area, and respectively detecting the skin moisture content and the percutaneous moisture loss before and after smearing the detection reagent on two groups of subjects of the control group and the experimental group for 6 hours.
Detection equipment:
CM 825 skin moisture probe, aquaFlux AF200 skin moisture loss tester.
Detection part: the inner sides of the forearms of the hands of the testes need to be uniformly cleaned before the testes are smeared, the inner sides of the forearms of the hands of the testes are wiped clean by dry facial tissues, the measurement area marks are needed to be made on the inner sides of the forearms of the hands of the testes, the area of a test area is 5cm multiplied by 5cm, 5 points in the test area are measured, and the average value of the measurement areas is calculated.
Detection reagent: the test agent applied to the control subjects was an aqueous solution containing 5%1, 3-propanediol and 0.5% glycerol. The test agent smeared on the experimental group experimental object is an aqueous solution containing 5% of 1, 3-propanediol, 0.5% of glycerol and 1% of cosmetic raw material;
detection result: is marked as A Control group-front 、A Control group-post 、A Experimental group-front And A Experimental group-post ；
(2) Calculating average value of each group, and analyzing cosmetic raw material moisture evaluation index B 1 And B 2 ，B 1 Corresponding to the moisture content of skin, B 2 In response to the loss of water through the skin,
example 2
The embodiment provides a method for testing the moisturizing effect of cosmetic raw materials, which comprises the following steps:
(1) A control group and an experimental group were set, each group of 10 persons (the age was 18-45 years old, the male and female were half, and any cosmetic, external medicine or oral health care product could not be used 3 days before the test of the tested part).
(2) And (3) smearing 1mL of detection reagent on the tested area, and respectively detecting the skin moisture content and the percutaneous moisture loss before and after smearing the detection reagent on two groups of subjects of the control group and the experimental group for 6 hours.
Detection equipment:
CM 825 skin moisture probe, aquaFlux AF200 skin moisture loss tester.
Detection part: the inner sides of the forearms of the hands of the testes need to be uniformly cleaned before the testes are smeared, the inner sides of the forearms of the hands of the testes are wiped clean by dry facial tissues, the measurement area marks are needed to be made on the inner sides of the forearms of the hands of the testes, the area of a test area is 5cm multiplied by 5cm, 5 points in the test area are measured, and the average value of the measurement areas is calculated.
Detection reagent: the test agent applied to the control subjects was an aqueous solution containing 1%1, 3-propanediol and 0.2% glycerol.
The test agent smeared on the experimental group experimental object is an aqueous solution containing 1% of 1, 3-propanediol, 0.2% of glycerol and 1% of cosmetic raw material;
detection result: is marked as A Control group-front 、A Control group-post 、A Experimental group-front And A Experimental group-post ；
(2) Calculating average value of each group, and analyzing cosmetic raw material moisture evaluation index B 1 And B 2 ，B 1 Corresponding to the moisture content of skin, B 2 In response to the loss of water through the skin,
example 3
The embodiment provides a method for testing the moisturizing effect of cosmetic raw materials, which comprises the following steps:
(1) A control group and an experimental group were set, each group of 10 persons (the age was 18-45 years old, the male and female were half, and any cosmetic, external medicine or oral health care product could not be used 3 days before the test of the tested part).
(2) And (3) smearing 1mL of detection reagent on the tested area, and respectively detecting the skin moisture content and the percutaneous moisture loss before and after smearing the detection reagent on two groups of subjects of the control group and the experimental group for 6 hours.
Detection equipment:
CM 825 skin moisture probe, aquaFlux AF200 skin moisture loss tester.
Detection part: before application, the subject needs to uniformly clean the inner sides of the forearms of the hands by wiping the inner sides with dry facial tissues. The inner sides of the forearms of the two hands of the subject are marked with measuring areas, the area of the test area is 5cm multiplied by 5cm, 5 points in the test area are measured, and the average value is calculated.
Detection reagent: the test agent applied to the control subjects was an aqueous solution containing 9.5%1, 3-propanediol and 0.5% glycerol. The test agent smeared on the experimental group experimental object is an aqueous solution containing 9.5 percent of 1, 3-propanediol, 0.5 percent of glycerol and 1 percent of cosmetic raw material;
detection result: is marked as A Control group-front 、A Control group-post 、A Experimental group-front And A Experimental group-post ；
(2) Calculating average value of each group, and analyzing cosmetic raw material moisture evaluation index B 1 And B 2 ，B 1 Corresponding to the moisture content of skin, B 2 In response to the loss of water through the skin,
example 4
The present example provides a method for testing the moisturizing effect of cosmetic raw materials, which is different from example 1 only in that the test reagents of the control group and the experimental group do not contain glycerol, and the mass parts of glycerol are distributed to 1, 3-propanediol, and the rest are the same as example 1.
Example 5
This example provides a method for testing the moisturizing effect of cosmetic raw materials, which is different from example 1 only in that the test reagents of the control group and the experimental group do not contain 1, 3-propanediol, and the mass parts of 1, 3-propanediol are distributed to glycerin, and the rest are the same as example 1.
Example 6
This example provides a method for testing the moisturizing effect of a cosmetic raw material, which is different from example 1 only in that 5% of 1, 3-propanediol and 0.5% of glycerin (the mass fraction of humectant in the aqueous humectant solution is 5.5%) are replaced with 0.5% of 1, 3-propanediol and 0.05% of glycerin (the mass fraction of humectant in the aqueous humectant solution is 0.55%), and the rest is the same as example 1.
Example 7
This example provides a method for testing the moisturizing effect of a cosmetic raw material, which differs from example 1 only in that 5% of 1, 3-propanediol and 0.5% of glycerin (the mass fraction of humectant in the aqueous humectant solution is 5.5%) are replaced with 11% of 1, 3-propanediol and 1.1% of glycerin (the mass fraction of humectant in the aqueous humectant solution is 11.1%), and the rest is the same as example 1.
Comparative example 1
The comparative example provides a method for testing the moisturizing effect of cosmetic raw materials, wherein the testing method is a 28-day human body moisturizing testing method and comprises the following specific steps:
(1) Selecting volunteers 10 (18-45 years old; men and women each half; no cosmetic, topical medicine or oral health product can be used 3 days before the test of the tested part).
(2) 1mL of detection reagent is smeared on the tested area twice a day, and the detection reagent is used for 28 days in the morning and at night. The skin moisture content and the percutaneous moisture loss of the subject before and after the test agent was applied for 28 days were measured, respectively.
Detection equipment:
CM 825 skin moisture probe, aquaFlux AF200 skin moisture loss tester.
Detection part: before application, the subject needs to uniformly clean the inner sides of the forearms of the hands by wiping the inner sides with dry facial tissues. The inner sides of the forearms of the two hands of the subject are marked with measuring areas, the area of the test area is 5cm multiplied by 5cm, 5 points in the test area are measured, and the average value is calculated.
Detection reagent: 1% by mass of an aqueous cosmetic raw material solution.
Detection result: cosmetic raw material moisturizing effect index C 1 And C 2 ，C 1 To control skin moisture content, C 2 In order to control the percutaneous moisture loss,
test example 1
The test example analyzes the detection result of the cosmetic raw material moisturizing effect test method and the detection result of the conventional moisturizing test method.
The specific analysis method comprises the following steps:
the cosmetic material is a mixture of sodium lactate and seaweed extract (the mass ratio of sodium lactate to seaweed extract is 1:4).
The cosmetic materials were all dissolved in the test agent, and the above-mentioned cosmetic materials were tested by the test methods described in examples and comparative examples, and the average values of the skin moisture content and the percutaneous moisture loss data of each group were calculated, respectively, and the results are shown in table 1.
TABLE 1
As can be seen from the data in Table 1, the moisture content and the percutaneous moisture loss data of the two groups of skin are similar to those of the comparative example 1, the comparative example 1 is a conventional cosmetic raw material moisturizing detection method, and the method is similar to the final result of the method of the comparative example 1, but the detection time of the detection method is short, so that compared with the conventional 28-day human body test, the time consumption of the method of the invention is obviously reduced, the instant moisturizing effect of the cosmetic raw material can be effectively verified, and the detection result is true and accurate.
Compared with example 5 and comparative example 1, example 4 shows that the humectant is one of 1, 3-propanediol and glycerin, and the skin moisture content and the absolute value data of percutaneous water dispersion are obviously lower than those of example 1 and comparative example 1, so that the two humectants are combined for use, and have a synergistic effect in improving the accuracy of the detection method.
Compared with example 7 and example 1, the moisture content and the absolute value data of the percutaneous water dispersion of the humectant are not in the range of 1-10%, and are obviously lower than those of example 1 and comparative example 1, so that the detection result of the moisture preservation method is more true and accurate when the preferred range of 1-10% is selected as the moisture content of the humectant.
Test example 2
The present test example evaluates the application range of the cosmetic raw material of the method for testing the moisturizing effect of the cosmetic raw material of the present invention. The method specifically comprises the steps of detecting the easily volatile and insoluble cosmetic raw materials, and evaluating the materials by the following steps:
(1) Cosmetic raw material
(1) The volatile cosmetic material is a mixture of dimethyl siloxane and seaweed extract (the mass ratio of dimethyl siloxane to seaweed extract is 10:1).
(2) The indissoluble cosmetic material is a mixture of sodium hyaluronate, vaseline and seaweed extract (the mass ratio of sodium hyaluronate, vaseline and seaweed extract is 6:1:0.5).
(2) The above cosmetic raw materials were examined by the examination methods described in example 1 and comparative example 1, respectively, and the average values of the skin moisture content and the percutaneous moisture loss data of each group were calculated, and the results are shown in table 2.
TABLE 2
As can be seen from the data in table 2, the method for testing the moisturizing effect of the cosmetic raw materials according to the present invention can be used for testing volatile and poorly water-soluble cosmetic raw materials, such as dimethylsiloxane with high volatile nature, poorly water-soluble vaseline and sodium hyaluronate.
The independent comparison of the detection results of the example 1- (1) group and the comparative example 1- (1), the example 1- (2) group and the comparative example 1- (2) group shows that the skin moisture content and the percutaneous moisture dispersion value of the example 1 are higher than those of the comparative example 1, and the detection data are more realistic compared with the conventional moisturizing detection method, the components consisting of 1, 3-propanediol and glycerol can effectively increase the solubility of the components of the cosmetics to be detected, reduce the volatilization loss of volatile components.
The applicant states that the present invention is illustrated by the above examples of the preparation process of the present invention, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must be practiced by relying on the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (10)
1. The method for testing the moisturizing effect of the cosmetic raw materials is characterized by comprising the following steps of:
(1) The skin moisture content and/or the percutaneous moisture loss before and after the application of the detection reagent are respectively detected for the two groups of subjects in the control group and the experimental group, and the result is recorded as A Control group-front 、A Control group-post 、A Experimental group-front And A Experimental group-post ；
Wherein the detection reagent smeared by the experimental subject of the control group is a humectant aqueous solution, and the detection reagent smeared by the experimental subject of the experimental group is an equal-concentration humectant aqueous solution containing cosmetic raw materials;
(2) Calculating a moisturizing evaluation index B of the raw materials of the cosmetics,
using the amplitude of increase in skin moisture content B 1 And/or the amplitude of the decrease in percutaneous moisture loss B 2 To evaluate the moisturizing effect of the cosmetics.
2. The method for testing the moisturizing effect of a cosmetic raw material according to claim 1, wherein the moisturizing agent of step (1) comprises any one or a combination of at least two of 1, 3-propanediol, glycerol, butanediol, methylpropanediol, lactic acid, sodium lactate, mannose, sorbitol, or trehalose.
3. The method for testing the moisturizing effect of a cosmetic raw material according to claim 1 or 2, wherein the moisturizing agent of step (1) comprises 1, 3-propanediol.
4. The method for testing the moisturizing effect of a cosmetic raw material of claim 3, wherein the moisturizing agent of step (1) further comprises glycerin.
5. The method for testing the moisturizing effect of a cosmetic raw material according to claim 4, wherein the mass ratio of 1, 3-propanediol to glycerol is (5-20): 1.
6. The method for testing the moisturizing effect of cosmetic raw materials according to any one of claims 1 to 5, wherein the mass fraction of the humectant in the aqueous humectant solution of step (1) is 1 to 10%.
7. The method for testing the moisturizing effect of a cosmetic raw material according to any one of claims 1 to 6, wherein the aqueous solution of an isoconcentrate moisturizing agent containing the cosmetic raw material in the step (1) has a mass fraction of the cosmetic raw material of 0.01 to 10%.
8. The method for testing the moisturizing effect of cosmetic raw materials according to any one of claims 1 to 7, wherein the detection of the skin moisture content and/or the transdermal moisture loss is performed within 2 to 10 hours after the application of the detection reagent in the step (1).
9. The method for testing the moisturizing effect of a cosmetic raw material according to any one of claims 1 to 8, wherein the cosmetic raw material comprises a solid cosmetic raw material, a liquid cosmetic raw material, or a gaseous cosmetic raw material.
10. Use of the cosmetic raw material moisturizing effect test method according to any one of claims 1 to 9 in cosmetic moisturizing effect evaluation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310166615.0A CN116183829A (en) | 2023-02-24 | 2023-02-24 | Cosmetic raw material moisturizing effect testing method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310166615.0A CN116183829A (en) | 2023-02-24 | 2023-02-24 | Cosmetic raw material moisturizing effect testing method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116183829A true CN116183829A (en) | 2023-05-30 |
Family
ID=86438014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310166615.0A Pending CN116183829A (en) | 2023-02-24 | 2023-02-24 | Cosmetic raw material moisturizing effect testing method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116183829A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117471045A (en) * | 2023-11-13 | 2024-01-30 | 广州美域医学检验有限公司 | Test method for rapidly evaluating moisturizing effect of cosmetic raw materials |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100035005A (en) * | 2008-09-25 | 2010-04-02 | 유한킴벌리 주식회사 | Cosmetic composition for kids with high moisturizing effects |
| CN103191030A (en) * | 2013-04-02 | 2013-07-10 | 厦门大学 | Application of chitosan in preparation of cosmetic |
| CN106726654A (en) * | 2017-04-06 | 2017-05-31 | 湖南御家化妆品制造有限公司 | A kind of xylitol moisture-keeping composition and its application in cosmetics |
| CN106880513A (en) * | 2017-04-06 | 2017-06-23 | 湖南御家化妆品制造有限公司 | A kind of biological polyoses moisture-keeping composition and its applied in cosmetics are prepared |
| CN106963665A (en) * | 2017-04-06 | 2017-07-21 | 湖南御家化妆品制造有限公司 | One group of four-dimensional moisturizing is combined and its is preparing the application of moisture-keeping cosmetics |
| CN108852939A (en) * | 2018-08-23 | 2018-11-23 | 无限极（中国）有限公司 | A kind of moisture-keeping composition and its cosmetics of preparation |
| CN112147140A (en) * | 2020-08-28 | 2020-12-29 | 南京新环检测科技有限公司 | Method for evaluating moisturizing effect of cosmetics |
-
2023
- 2023-02-24 CN CN202310166615.0A patent/CN116183829A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100035005A (en) * | 2008-09-25 | 2010-04-02 | 유한킴벌리 주식회사 | Cosmetic composition for kids with high moisturizing effects |
| CN103191030A (en) * | 2013-04-02 | 2013-07-10 | 厦门大学 | Application of chitosan in preparation of cosmetic |
| CN106726654A (en) * | 2017-04-06 | 2017-05-31 | 湖南御家化妆品制造有限公司 | A kind of xylitol moisture-keeping composition and its application in cosmetics |
| CN106880513A (en) * | 2017-04-06 | 2017-06-23 | 湖南御家化妆品制造有限公司 | A kind of biological polyoses moisture-keeping composition and its applied in cosmetics are prepared |
| CN106963665A (en) * | 2017-04-06 | 2017-07-21 | 湖南御家化妆品制造有限公司 | One group of four-dimensional moisturizing is combined and its is preparing the application of moisture-keeping cosmetics |
| CN108852939A (en) * | 2018-08-23 | 2018-11-23 | 无限极（中国）有限公司 | A kind of moisture-keeping composition and its cosmetics of preparation |
| CN112147140A (en) * | 2020-08-28 | 2020-12-29 | 南京新环检测科技有限公司 | Method for evaluating moisturizing effect of cosmetics |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117471045A (en) * | 2023-11-13 | 2024-01-30 | 广州美域医学检验有限公司 | Test method for rapidly evaluating moisturizing effect of cosmetic raw materials |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2799865B1 (en) | Method for evaluating cosmetic | |
| CN116183829A (en) | Cosmetic raw material moisturizing effect testing method and application thereof | |
| CN111214386A (en) | Soothing and repairing composition containing ectoin and application thereof | |
| CN104251841A (en) | Multi-sample breath analyzer based on cavity ring-down spectroscopy | |
| US11365397B2 (en) | Methods for screening personal care products | |
| TWI596338B (en) | Method of evaluating the stratum corneum state and evaluation method of improving the stratum corneum of the cosmetic material | |
| CN113876633B (en) | Natural vegetable fat compound formed by human body sebum lipid imitation and preparation method and application thereof | |
| Xia et al. | Conductometric titration to determine total volatile basic nitrogen (TVB-N) for post-mortem interval (PMI) | |
| CN110496090B (en) | Composition and application thereof to preparation of skin care product for repairing skin damaged all night | |
| Chen et al. | Relationships among growth rate, vessel lumen area, and wood permeability for three central hardwood species | |
| CN110412022A (en) | A kind of quickly detection organic solid castoff NH3The gel test paper and its application method of volatility | |
| CN116850081A (en) | Repairing composition for sensitive muscles, freeze-dried mask and preparation method thereof | |
| CN115154347B (en) | Cleansing cream and preparation method thereof | |
| WO2014108758A1 (en) | Nanoparticles dispersion for detection of skin health conditions and a diagnostic kit thereof | |
| JP2019202996A (en) | COSMETIC COMPOSITION COMPRISING γ-POLYGLUTAMIC ACID AS ACTIVE COMPONENT | |
| CN113876614B (en) | Glycyrrhizin and tremella aurantialba polysaccharide degradation product compound composition and application thereof | |
| JP2008039761A (en) | Evaluating method of square layer | |
| CN115317391A (en) | Anti-wrinkle composition, anti-wrinkle essence cream applied to anti-wrinkle composition and preparation method of anti-wrinkle essence cream | |
| CN116942584B (en) | Facial mask containing dendrobium candidum and polypeptide and preparation method thereof | |
| CN114668682B (en) | Moisturizing and skin-brightening composition and application thereof | |
| CN106236639A (en) | A kind of cosmetic composition | |
| CN117471045A (en) | Test method for rapidly evaluating moisturizing effect of cosmetic raw materials | |
| GAYLARDE et al. | Reductions in breath ethanol readings in normal male volunteers following mouth rinsing with water at differing temperatures | |
| CN117137838A (en) | Synergistic anti-corrosion whitening composition and preparation method thereof | |
| CN113679651A (en) | Anti-aging composition, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |