CN116162534B - Ovum maturation culture dish - Google Patents
Ovum maturation culture dish Download PDFInfo
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- CN116162534B CN116162534B CN202310049699.XA CN202310049699A CN116162534B CN 116162534 B CN116162534 B CN 116162534B CN 202310049699 A CN202310049699 A CN 202310049699A CN 116162534 B CN116162534 B CN 116162534B
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- culture dish
- ovum
- seat
- groove
- stacking
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- 210000004681 ovum Anatomy 0.000 title claims abstract description 175
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 167
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 167
- 230000035800 maturation Effects 0.000 title claims abstract description 27
- 238000002372 labelling Methods 0.000 claims description 25
- 230000005540 biological transmission Effects 0.000 claims description 24
- 238000000926 separation method Methods 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 9
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 5
- 235000017491 Bambusa tulda Nutrition 0.000 claims 5
- 241001330002 Bambuseae Species 0.000 claims 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 5
- 239000011425 bamboo Substances 0.000 claims 5
- 230000000694 effects Effects 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- 238000000338 in vitro Methods 0.000 abstract description 3
- 235000013601 eggs Nutrition 0.000 description 16
- 230000002349 favourable effect Effects 0.000 description 10
- 239000012188 paraffin wax Substances 0.000 description 7
- 230000004720 fertilization Effects 0.000 description 6
- 230000009027 insemination Effects 0.000 description 5
- 238000000520 microinjection Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000007726 management method Methods 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 210000005239 tubule Anatomy 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000309464 bull Species 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000035606 childbirth Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000007425 progressive decline Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Abstract
The invention discloses an ovum maturation culture dish, and relates to the technical field of ovum culture dishes. The culture dish comprises a placement seat, wherein the surface of the placement seat is provided with culture dish components, the number of the culture dish components is four, the top of the placement seat is provided with placement grooves, the number of the placement grooves is four, and the placement grooves are matched with the culture dish components; the invention is convenient for medical staff to cultivate a plurality of ova, is beneficial to the medical staff to cultivate the ova better in the placement seat, improves the cultivation quality of the ova, improves the cultivation effect of the ova in the culture dish, is convenient for the medical staff to operate and use the culture dish, is beneficial to the medical staff to inject the ova into the culture dish for cultivation, and is beneficial to improving the stability of the culture dish in the use process, ensuring the stable cultivation environment of the ova in the culture dish and improving the quality of the in-vitro cultivation of the ova.
Description
Technical Field
The invention relates to the technical field of ovum culture dishes, in particular to an ovum maturation culture dish.
Background
Age may lead to a progressive decline in the quality of the woman's ova. The preparation of pregnancy in the best childbirth period has more positive influence on fertility, delivery and the like. The preparation for pregnancy between the ages of 23 and 30 is the optimal period for cultivating high quality ovum. A dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disk-shaped bottom and a lid, generally made of glass or plastic. The materials of the culture dishes are basically divided into two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells. The plastic may be polyethylene material, has disposable and multiple use, is suitable for laboratory inoculation, streaking and bacteria separation, and may be used in plant material cultivation.
The present Chinese patent with publication number CN201310596544.4 discloses a microscopic insemination culture dish, which is characterized in that the bottom of the microscopic insemination culture dish is provided with an inclined microscopic insemination culture dish, so that the front end of a microinjection needle still forms an included angle with the bottom of the dish when forming a horizontal angle, and if the needle point at the moment touches the bottom, the rear part of the front end of the microinjection needle cannot touch the bottom to cause the needle point to warp upwards to separate from sperm at the bottom, thereby smoothly grabbing and pressing the sperm and realizing normal microscopic insemination operation. Because the front end of the microinjection needle is operated at a horizontal angle, the microinjection needle can be used for carrying out microinjection on the ovum, and the risk of ovum disintegration caused by non-positive puncture in the traditional method is avoided.
At present, in the process of taking out an ovum and conducting fertilization cultivation, the ovum is usually placed in a cultivation groove of a culture dish for cultivation, the culture dish in the invention can conduct microscopic insemination on the ovum by using a microscopic injection needle, and the quality of ovum cultivation is improved, but in the cultivation process, the immature, overripe or polluted ovum is placed in the same fertilization groove together by the device, so that the fertilization of the mature ovum is negatively affected to a certain extent, the quality of in-vitro culture of the ovum is affected, meanwhile, the culture dish structure in the invention is single, the stable operation of the culture dish is not facilitated for medical staff, the convenience of the operation of the medical staff is affected, the cultivation staff is inconvenient to conduct cultivation operation on a plurality of ovum simultaneously, and the efficiency of ovum cultivation is reduced.
Disclosure of Invention
The invention provides an ovum maturation culture dish which has the advantages of improving the ovum culture quality and improving the use effect of the culture dish, thereby solving the problems in the prior art.
In order to achieve the above purpose, the invention is realized by the following technical scheme: the utility model provides an ovum maturation culture dish, is including placing the seat, the surface of placing the seat is provided with culture dish subassembly, and culture dish subassembly's quantity is four sets of, place the top of seat and seted up and placed the recess, place the quantity of recess and four, and place the recess and use with culture dish subassembly cooperation, the internally mounted who places the seat has adjusting part, and adjusting part and place the recess and pair and use, the internally mounted who places the recess has spacing subassembly, spacing subassembly's quantity is the multiunit, and spacing subassembly and culture dish subassembly cooperation use, place the surface of seat and seted up and placed the annular, and place the quantity of annular and be four.
The invention relates to an ovum maturation culture dish, which comprises an ovum culture dish seat, a holding ring, an ovum culture dish cover, a holding plate, a sequence number labeling area, a stacking groove, a limiting strip, a dish cover skirt, an azimuth mark, a stacking bottom plate, a stacking convex ring, a sperm groove, an ovum groove, a flat needle slope, a through groove, a separation measuring plate, a clamping strip, a clamping groove and a liquid injection ring groove, wherein the ovum culture dish seat is arranged in the placing groove, the ovum culture dish seat is arranged on an adjusting assembly, the holding ring is fixedly arranged on the ovum culture dish seat, the ovum culture dish cover is arranged on the ovum culture dish seat, the number of the holding plates is two, the two holding plates are respectively and fixedly connected to two sides of the ovum culture dish cover, the sequence number labeling area is arranged on one side of the ovum culture dish cover, the stacking groove is arranged at the bottom of the ovum culture dish cover, the limit strips are fixedly arranged on the surface of the stacking groove, the skirt edge of the dish cover is arranged on the top of the ovum culture dish cover, the azimuth mark is arranged on the surface of the stacking groove, the stacking bottom plate is fixedly arranged at the bottom of the ovum culture dish seat, the stacking convex rings are fixedly connected to the bottom of the stacking bottom plate, the sperm grooves are arranged in the ovum culture dish seat, the number of the sperm grooves is two, the ovum grooves are arranged in the ovum culture dish seat, the number of the sperm grooves is a groove, the flat needle slope is arranged in the ovum culture dish seat, the number of the flat needle slope is a plurality, the through grooves are arranged in the ovum culture dish seat, the number of the through grooves is a plurality, one end of each through groove is communicated with the sperm groove, the other end of each through groove is communicated with the ovum groove, the separation measuring plate is fixedly arranged in the ovum culture dish seat, the clamping strip is fixedly arranged on the surface of the ovum culture dish seat, the clamping groove is formed in the ovum culture dish cover and is matched with the clamping strip for use, and the liquid injection ring groove is formed in the culture dish seat.
The adjusting component comprises a transmission rotating rod, an adjusting knob, a transmission gear, a threaded rotating rod, a driven gear, a threaded sliding pipe and a jacking circular plate, wherein the transmission rotating rod is arranged in a placing seat, the adjusting knob is fixedly connected to one end of the transmission rotating rod, the transmission gear is fixedly arranged on the transmission rotating rod, the threaded rotating rod is arranged in the placing seat, the number of the threaded rotating rods is four, the driven gear is fixedly arranged on the threaded rotating rod, the threaded sliding pipe is in threaded connection with the threaded rotating rod, the jacking circular plate is fixedly arranged on the threaded sliding pipe, and the jacking circular plate is positioned at the bottom of the placing groove.
As the ovum maturation culture dish provided by the invention, the limiting assembly comprises the sliding cylinders, the limiting sliding rings, the reset springs, the limiting post rods and the limiting arc plates, wherein the sliding cylinders are fixedly arranged in the placement seat, the number of the sliding cylinders is a plurality of, the limiting sliding rings are in sliding connection in the sliding cylinders, the reset springs are arranged between the sliding cylinders and the limiting sliding rings, the limiting post rods are fixedly connected to the limiting sliding rings, the limiting arc plates are fixedly arranged at one ends, far away from the limiting sliding rings, of the limiting post rods, and the limiting arc plates are matched with the ovum culture dish seat for use.
As the ovum maturation culture dish, both sides of the surface of the placement seat are provided with the holding grooves, the bottom of the placement seat is fixedly provided with the two supporting seats, and the bottom of the supporting seats is provided with the anti-skid grains.
As the ovum maturation culture dish, the edge of the holding ring is provided with wavy easy-to-hold lines, the number of the clamping strips and the clamping grooves is multiple, and the clamping strips and the clamping grooves are distributed at equal intervals.
As the ovum maturation culture dish, one side of the surface of the placement seat is provided with the label labeling area, the label labeling area is of a frosted label labeling area, and the label labeling area and the serial number labeling area are of the same type.
As the ovum maturation culture dish, the types of the azimuth marks are digital azimuth marks, the number of the marks is 3, 6, 9 and 12, and the two sides of the surface of the separation measuring plate are provided with culture oil scales.
As the ovum maturation culture dish, the height of the dish cover skirt edge is 2mm, the dish cover skirt edge is matched with the ovum culture dish seat for use, and the stacking groove is matched with the bottom of the stacking bottom plate.
As the ovum maturation culture dish, the surfaces of the four placement grooves are fixedly provided with the limit rings, the limit rings are arranged in a matched mode with the stacking convex rings, and the stacking convex rings are matched with the limit strips.
The invention provides an ovum maturation culture dish. The beneficial effects are as follows:
the egg maturation culture dish is characterized in that the egg maturation culture dish comprises a placement seat, a culture dish assembly, a placement groove, an adjusting assembly, a limiting assembly and a placement ring groove, so that medical staff can conveniently culture a plurality of eggs, the medical staff can better culture the eggs in the placement seat, the culture quality of the eggs is improved, the culture effect of the eggs in the culture dish is improved, meanwhile, the egg maturation culture dish is convenient for the medical staff to operate and use the culture dish, the medical staff can conveniently inject the eggs into the culture dish to culture the eggs, the stability of the culture dish in the use process can be improved, the eggs in the culture dish can be guaranteed to be in a stable culture environment, and the in-vitro culture quality of the eggs is improved.
Drawings
FIG. 1 is a schematic perspective view of the structure of the present invention;
FIG. 2 is a perspective view of a first partial structure of the present invention;
FIG. 3 is a perspective view of a second partial structure of the present invention;
FIG. 4 is a perspective view of a third partial structure of the present invention;
FIG. 5 is a perspective exploded view of a first partial structure of the present invention;
FIG. 6 is a perspective exploded view of a second partial structure of the present invention;
FIG. 7 is a cross-sectional view of a first partial structure of the present invention;
FIG. 8 is a perspective view of a fourth partial structure of the present invention;
FIG. 9 is a perspective view of a fifth partial structure of the present invention;
FIG. 10 is an enlarged view of the structure of FIG. 4A in accordance with the present invention;
fig. 11 is an enlarged view of the structure at B in fig. 7 according to the present invention.
In the figure: 1. a placement seat; 2. a culture dish assembly; 201. an ovum culture dish seat; 202. a grip ring; 203. an ovum culture dish cover; 204. holding the plate; 205. a serial number labeling area; 206. a stacking groove; 207. a limit bar; 208. a dish cover skirt; 209. azimuth identification; 210. stacking a bottom plate; 211. stacking convex rings; 212. a sperm tank; 213. egg grooves; 214. a flat needle slope; 215. a through groove; 216. separating the measuring plates; 217. a clamping strip; 218. a clamping groove; 219. a liquid injection ring groove; 3. a support base; 4. a label labeling area; 5. holding the groove; 6. placing the groove; 7. an adjustment assembly; 701. a transmission rotating rod; 702. an adjustment knob; 703. a transmission gear; 704. a threaded rotating rod; 705. a driven gear; 706. a threaded slide tube; 707. lifting the circular plate; 8. a limit ring; 9. a limit component; 901. a slide cylinder; 902. a limiting slip ring; 903. a return spring; 904. limiting the post rod; 905. limiting arc plates; 10. and placing the ring groove.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-11, the present invention provides a technical solution: including placing seat 1, the surface of placing seat 1 is provided with culture dish subassembly 2, and culture dish subassembly 2's quantity is four sets, place the top of seat 1 and offered and placed recess 6, place recess 6's quantity and four, and place recess 6 and culture dish subassembly 2 cooperation and use, the internally mounted of placing seat 1 has adjusting part 7, and adjusting part 7 and place recess 6 pair and use, the internally mounted of placing recess 6 has spacing subassembly 9, the quantity of spacing subassembly 9 is the multiunit, and spacing subassembly 9 and culture dish subassembly 2 cooperation use, place the surface of seat 1 and offered and placed annular 10, and place annular 10's quantity is four.
In the embodiment, through setting up of placing seat 1, culture dish subassembly 2, placing recess 6, adjusting part 7, spacing subassembly 9 and placing annular 10, can make things convenient for medical staff to cultivate a plurality of eggs, be favorable to medical staff to cultivate the ovum better in placing seat 1, improve the cultivation quality of a plurality of eggs, promote the culture effect of ovum in the culture dish, simultaneously the invention can also make things convenient for medical staff to operate the culture dish and use, be favorable to medical staff to pour into the ovum into the culture dish and cultivate, and can also improve the stability of culture dish in the use, be favorable to guaranteeing that the ovum is in stable cultivation environment in the culture dish, improve the quality of ovum external culture.
Specifically, the culture dish assembly 2 comprises an ovum culture dish seat 201, a holding ring 202, an ovum culture dish cover 203, a holding plate 204, a serial number labeling area 205, a stacking groove 206, a limit strip 207, a dish cover skirt 208, an azimuth mark 209, a stacking bottom plate 210, a stacking convex ring 211, a sperm groove 212, an ovum groove 213, a flat slope 214, a through groove 215, a separation measuring plate 216, a clamping strip 217, a clamping groove 218 and a liquid injection ring groove 219, wherein the ovum culture dish seat 201 is arranged in the placing groove 6, the ovum culture dish seat 201 is positioned on the adjusting assembly 7, the holding ring 202 is fixedly arranged on the ovum culture dish seat 201, the ovum culture dish cover 203 is arranged on the ovum culture dish seat 201, the number of the holding plates 204 is two, the two holding plates 204 are respectively and fixedly connected to two sides of the ovum culture dish cover 203, the serial number labeling area 205 is arranged on one side of the ovum culture dish cover 203, the stacking groove 206 is arranged at the bottom of the ovum culture dish cover 203, the limit bars 207 are fixedly arranged on the surface of the stacking groove 206, the dish cover skirt 208 is arranged on the top of the ovum culture dish cover 203, the azimuth mark 209 is arranged on the surface of the stacking groove 206, the stacking bottom plate 210 is fixedly arranged on the bottom of the ovum culture dish seat 201, the stacking convex rings 211 are fixedly connected to the bottom of the stacking bottom plate 210, the sperm grooves 212 are arranged in the ovum culture dish seat 201, the number of the sperm grooves 212 is two, the ovum grooves 213 are arranged in the ovum culture dish seat 201, the number of the sperm grooves 212 is 6, the flat needle slopes 214 are arranged in the ovum culture dish seat 201, the number of the flat needle slopes 214 is a plurality, the through grooves 215 are arranged in the ovum culture dish seat 201, the number of the through grooves 215 is a plurality, one end of the through grooves 215 is communicated with the sperm grooves 212, the other end of the through grooves 215 is communicated with the sperm grooves 213, the separation measuring plates 216 are fixedly arranged in the ovum culture dish seat 201, the locking strip 217 is fixedly mounted on the surface of the ovum culture dish base 201, the locking groove 218 is formed in the ovum culture dish cover 203, the locking groove 218 is matched with the locking strip 217 for use, and the liquid injection ring groove 219 is formed in the culture dish base 201.
In this embodiment, through the arrangement of the ovum culture dish base 201, the holding ring 202, the ovum culture dish cover 203, the holding plate 204, the serial number labeling area 205, the stacking groove 206, the limit bar 207, the dish cover skirt 208, the azimuth mark 209, the stacking bottom plate 210, the stacking convex ring 211, the sperm groove 212, the ovum groove 213, the flat needle slope 214, the through groove 215, the separation measuring plate 216, the clamping strip 217 and the clamping groove 218, the ovum culture dish base 201 can be conveniently used for injecting the ovum into the ovum groove 213 of the ovum culture dish base 201 by medical staff for culturing, the separation culture of a plurality of the ovum by medical staff is facilitated, the ovum culture effect is improved, and the ovum culture operation is conveniently carried out by the medical staff, the convenience is provided for the medical staff, the difficulty of the operation of the medical staff is reduced, the quality of the ovum operation culture is improved, and meanwhile, the use of the culture dish can be conveniently taken by the medical staff, and the use effect of the culture dish operation is improved.
Specifically, the adjusting assembly 7 includes a transmission rotating rod 701, an adjusting knob 702, a transmission gear 703, a threaded rotating rod 704, a driven gear 705, a threaded sliding tube 706 and a lifting circular plate 707, wherein the transmission rotating rod 701 is installed in the placement seat 1, the adjusting knob 702 is fixedly connected to one end of the transmission rotating rod 701, the transmission gear 703 is fixedly installed on the transmission rotating rod 701, the threaded rotating rod 704 is installed in the placement seat 1, the number of the threaded rotating rods 704 is four, the driven gear 705 is fixedly installed on the threaded rotating rod 704, the threaded sliding tube 706 is in threaded connection with the threaded rotating rod 704, the lifting circular plate 707 is fixedly installed on the threaded sliding tube 706, and the lifting circular plate 707 is located at the bottom of the placement groove 6.
In this embodiment, through the setting of transmission bull stick 701, adjust knob 702, drive gear 703, screw thread bull stick 704, driven gear 705, screw thread slide 706 and jacking plectane 707, can conveniently adjust the culture dish subassembly 2 in placing recess 6, be favorable to placing the height of culture dish subassembly 2 in the recess 6 and adjust, make things convenient for medical staff to place or take culture dish subassembly 2, provide convenience for medical staff, promote the effect that a plurality of culture dish subassemblies 2 were placed and are used.
Specifically, spacing subassembly 9 includes a slide tube 901, spacing sliding ring 902, reset spring 903, spacing post 904 and spacing arc board 905, slide tube 901 fixed mounting is in placing seat 1, and the quantity of slide tube 901 is a plurality of, spacing sliding ring 902 sliding connection is in slide tube 901, reset spring 903 installs between slide tube 901 and spacing sliding ring 902, spacing post 904 fixed connection is on spacing sliding ring 902, spacing arc board 905 fixed mounting is kept away from the one end of spacing post 904 of spacing sliding ring 902, and spacing arc board 905 and ovum culture dish seat 201 cooperation use.
In this embodiment, through the setting of slide cylinder 901, spacing sliding ring 902, reset spring 903, spacing pole 904 and spacing arc board 905, can conveniently carry out spacing fixedly to the culture dish subassembly 2 of placing in recess 6, be favorable to improving the spacing effect that culture dish subassembly 2 placed in placing recess 6, improve the stability of culture dish subassembly 2 in placing the use, reduce the possibility that culture dish subassembly 2 cultivates and appears rocking by a wide margin or empting in the use, promote the ovum effect of cultivateing.
Specifically, holding grooves 5 are formed in two sides of the surface of the placement seat 1, two support seats 3 are fixedly arranged at the bottom of the placement seat 1, and anti-slip lines are formed in the bottom of the support seats 3.
In this embodiment, through holding recess 5's setting, can make things convenient for medical personnel to hold placing seat 1, improve medical personnel and remove stability when placing seat 1, through the setting of supporting seat 3, can improve and place seat 1 and place the stability of cultivateing, be favorable to promoting the steadiness of placing when seat 1 places, reduce and place seat 1 and appear gliding condition.
Specifically, the edge of the grip ring 202 is provided with wavy easy-to-grip lines, the number of the clamping strips 217 and the clamping grooves 218 is multiple, and the clamping strips 217 and the clamping grooves 218 are all distributed at equal intervals.
In this embodiment, through the setting of gripping ring 202, can make things convenient for medical personnel to grip ring 202, be favorable to medical personnel to grip to take ovum culture dish seat 201, improve medical personnel and hold the stability of ovum culture dish seat 201, through the setting of block strip 217 and block groove 218, can improve the accuracy of ovum culture dish seat 201 and ovum culture dish lid 203 installation, promote the effect of ovum culture dish seat 201 and ovum culture dish lid 203 block.
Specifically, a label labeling area 4 is arranged on one side of the surface of the placement seat 1, the label labeling area 4 is a frosted label labeling area, and the label labeling area 4 and the serial number labeling area 205 are the same in kind.
In this embodiment, through the setting of label subsides mark district 4, can conveniently carry out the label setting to placing seat 1, be favorable to medical personnel to place seat 1 and use management, through the setting of serial number subsides mark district 205, can sort the setting to the culture dish, be favorable to medical personnel to manage a plurality of culture dishes, improve the culture effect of ovum culture dish.
Specifically, the azimuth mark 209 is a digital azimuth mark, and the numbers of the marks are 3, 6, 9 and 12, and the two sides of the surface of the separation measuring plate 216 are provided with culture oil scales.
In this embodiment, through the setting of position sign 209, be favorable to the position to the culture dish to sign, make things convenient for medical personnel to control the position of cultivating the ovum in the culture dish subassembly 2, improve medical personnel's treatment effect to the ovum is cultivateed, through separating the setting of survey board 216, can divide into two sets of culture areas with ovum culture dish seat 201, improve the management effect that the ovum was cultivateed to can also make things convenient for medical personnel to control the interpolation to ovum cultivation oil, improve the quality that the ovum was cultivateed.
Specifically, the height of the dish cover skirt 208 is 2mm, and the dish cover skirt 208 is matched with the ovum culture dish base 201, and the stacking groove 206 is matched with the bottom of the stacking bottom plate 210.
In this embodiment, through the setting of dish lid shirt rim 208, can improve the spacing effect to ovum culture dish seat 201, promote a plurality of culture dish subassemblies 2 to stack the setting, promote culture dish subassembly 2's result of use, through stacking groove 206 and stacking bottom plate 210's setting, can be better stack the use to a plurality of culture dish subassemblies 2, promote the stability that culture dish subassembly 2 was placed.
Specifically, the surfaces of the four placement grooves 6 are fixedly provided with limit rings 8, the limit rings 8 are matched with the stacking convex rings 211, and the stacking convex rings 211 are matched with the limit strips 207.
In this embodiment, through setting up of spacing ring 8, can improve the stability that ovum culture dish seat 201 placed in placing recess 6, improve the spacing installation effect to stacking bottom plate 210, through stacking bulge loop 211 and spacing 207's setting, can also conveniently stack to stacking bottom plate 210 in stacking groove 206, improve the culture dish and stack the effect of using.
When the egg sterilizing device is used, firstly, medical staff performs comprehensive sterilization treatment on the placing seat 1 and the culture dish assemblies 2 before culturing eggs, and then performs airtight protection on the treated placing seat 1 and culture dish assemblies 2 through sealing sleeves, and at the moment, a plurality of culture dish assemblies 2 are stacked;
when the preparation is completed, firstly, the medical staff opens the sealing sleeve on the surface of the culture dish assembly 2, then removes one culture dish assembly 2 on the top of the stacked culture dishes, then the medical staff takes the ovum culture dish cover 203 out of the ovum culture dish seat 201 through the holding plate 204, and then the medical staff drops the small drops of the culture solution into the sperm groove 212 and the ovum grooves 213 around the sperm groove 212, and the fertilization culture solution is 37 ℃ and 6% CO 2 After the conditions are balanced, the culture solution in the sperm groove 212 and the ovum groove 213 is drained into the through groove 215, then the culture solution in the sperm groove 212 and the ovum groove 213 is communicated along the through groove 215, then the paraffin culture oil is added into the ovum culture dish seat 201 by medical staff through the tubule, at the moment, one end of the tubule is placed in the liquid injection ring groove 219, then the paraffin culture oil is led into the through groove 215 through the tubule, and when the paraffin culture oil is in the following conditionAfter the covering in the through groove 215, the paraffin culture oil is fully diffused to the middle part of the ovum culture dish seat 201, then the paraffin culture oil is continuously added until the height of the paraffin culture oil reaches the scale mark on the separation measuring plate 216, at the moment, the paraffin culture oil covered in the dish is 3-3.5 milliliters, which is beneficial to the constant temperature in the external operation transfer process, then the ovum taken out by a doctor is placed into the ovum groove 213 through a flat needle, then the sperm is placed into the sperm groove 212 by the doctor, in the process, 1-2 ovum are placed into each ovum groove 213, corresponding sperm is placed into each sperm groove 212 (the adding sperm amount with a volume of 10-20uL is calculated according to the sperm density), at the moment, when the flat needle is used for injection, the flat needle is inserted into the flat needle along the flat needle slope 214, which is beneficial to guaranteeing the quality of the ovum and sperm injection, then medical staff covers the ovum culture dish cover 203 on the ovum culture dish seat 201, at the moment, the clamping groove 218 in the ovum culture dish cover 203 and the clamping bar 217 on the surface of the ovum culture dish seat 201 are opposite to Ji Kage, then medical staff pastes the serial number label of the culture dish in the serial number labeling area 205, then the medical staff places the culture dish in the placing groove 6 on the surface of the placing seat 1 by holding the holding ring 202, at the moment, the stacking bottom plate 210 is positioned on the jacking circular plate 707, the stacking convex ring 211 at the bottom of the stacking bottom plate 210 is correspondingly arranged with the limiting ring 8, when the culture dish assembly 2 is placed in the placing groove 6, the limiting clamping is carried out on the culture dish assembly 2 by the limiting assembly 9, at the moment, the limiting sliding ring 902 and the limiting post 904 are ejected out by the reaction force of the reset spring 903, the limiting post rod 904 drives the limiting arc plate 905 to move to the surface of the ovum culture dish seat 201, the ovum culture dish seat 201 is limited and fixed through the limiting arc plate 905, the holding ring 202 is positioned at the placement ring groove 10, medical staff can adjust the culture dish according to the azimuth mark 209 on the ovum culture dish cover 203, management operation is facilitated for the subsequent medical staff, the culture coordinates label is attached to the label labeling area 4, the medical staff holds the placement seat 1 to move into the incubator through the holding groove 5, the anti-slip strength of placement seat 1 is improved through the supporting seat 3, the environment of human uterine cavity is simulated through the incubator, and the culture dish is cultivatedTransfer of Assembly 2 to 37 ℃, 6% C0 2 Equilibrated overnight in incubator;
the medical staff then takes the placing seat 1 out of the incubator, then drives the transmission rotating rod 701 and the transmission gear 703 to rotate through rotating the adjusting knob 702, then drives the plurality of screw rotating rods 704 to rotate simultaneously through meshing of the transmission gear 703 and the driven gear 705, then the screw sliding tube 706 ascends on the surface of the screw rotating rods 704, then the screw sliding tube 706 drives the jacking circular plate 707 to push the culture dish assembly 2 upwards for a certain distance, then the medical staff takes the culture dish out through holding the holding ring 202, then the medical staff performs fertilization examination on the ovum in the ovum slot 213 in the ovum culture dish seat 201, in addition, the channel fertilization culture dish can also be used for single embryo/embryo combined culture and pgd/pgs biopsy culture, then the doctor puts the fertilized ovum successfully cultured into a red tubular container to wait for cooling, and finally the doctor puts the fertilized ovum into liquid nitrogen for freezing storage.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to substitute or change the technical scheme and the novel concept of the present invention within the scope of the present invention.
Claims (9)
1. An ovum maturation culture dish, its characterized in that: including placing seat (1), the surface of placing seat (1) is provided with culture dish subassembly (2), and culture dish subassembly (2) quantity is four sets of, place the top of seat (1) and seted up and place recess (6), place recess (6) quantity and use with culture dish subassembly (2) cooperation, the internally mounted of placing seat (1) has adjusting part (7), and adjusting part (7) use with placing recess (6) pair, the internally mounted of placing recess (6) has spacing subassembly (9), the quantity of spacing subassembly (9) is the multiunit, and spacing subassembly (9) use with culture dish subassembly (2) cooperation, place annular (10) have been seted up on the surface of placing seat (1), and place the quantity of annular (10) and be four.
The culture dish component (2) comprises an ovum culture dish seat (201), a holding ring (202), an ovum culture dish cover (203), a holding plate (204), a sequence number labeling area (205), a stacking groove (206), a limit bar (207), a dish cover skirt (208), an azimuth mark (209), a stacking bottom plate (210), a stacking convex ring (211), a sperm groove (212), an ovum groove (213), a flat needle slope (214), a through groove (215), a separation measuring plate (216), a clamping bar (217), a clamping groove (218) and a liquid injection ring groove (219), wherein the ovum culture dish seat (201) is arranged in a placing groove (6), the ovum culture dish seat (201) is positioned on an adjusting component (7), the holding ring (202) is fixedly arranged on the ovum culture dish seat (201), the number of the holding plate (204) is two, the two holding plates (204) are respectively fixedly connected with the ovum culture dish seat (203), the ovum culture dish seat (203) is fixedly arranged on the two sides of the ovum culture dish seat (201), the ovum culture dish seat (201) is fixedly arranged on the limit bar (203), the ovum culture dish seat (201) is fixedly arranged on the ovum culture dish seat (201), the dish cover skirt (208) is arranged at the top of the ovum culture dish cover (203), the azimuth mark (209) is arranged on the surface of the stacking groove (206), the stacking bottom plate (210) is fixedly arranged at the bottom of the ovum culture dish seat (201), the stacking convex ring (211) is fixedly connected at the bottom of the stacking bottom plate (210), the groove (212) is arranged in the ovum culture dish seat (201), the number of the sperm grooves (212) is two, the ovum grooves (213) are arranged in the ovum culture dish seat (201), the number of the sperm grooves (212) is the number of the placing grooves (6), the flat needle slope (214) is arranged in the ovum culture dish seat (201), the number of the flat needle slope (214) is a plurality, the through groove (215) is arranged in the ovum culture dish seat (201), one end of the through groove (215) is communicated with the groove (212), the other end of the through groove (215) is communicated with the sperm groove (217) by the clamping groove (216), the sperm clamping groove (217) is fixedly arranged on the surface of the ovum culture dish seat (201) by the clamping strip (218) and is fixedly arranged on the surface of the ovum culture dish seat (203), the liquid injection ring groove (219) is arranged in the culture dish base (201).
2. An ovum maturation culture dish according to claim 1, wherein: the adjusting assembly (7) comprises a transmission rotating rod (701), an adjusting knob (702), a transmission gear (703), a threaded rotating rod (704), a driven gear (705), a threaded sliding tube (706) and a jacking circular plate (707), wherein the transmission rotating rod (701) is installed in the placement seat (1), the adjusting knob (702) is fixedly connected to one end of the transmission rotating rod (701), the transmission gear (703) is fixedly installed on the transmission rotating rod (701), the threaded rotating rod (704) is installed in the placement seat (1), the number of the threaded rotating rods (704) is four, the driven gear (705) is fixedly installed on the threaded rotating rod (704), the threaded sliding tube (706) is in threaded connection with the threaded rotating rod (704), the jacking circular plate (707) is fixedly installed on the threaded sliding tube (706), and the jacking circular plate (707) is located at the bottom of the placement groove (6).
3. An ovum maturation culture dish according to claim 1, wherein: limiting component (9) are including a slide section of thick bamboo (901), spacing sliding ring (902), reset spring (903), spacing pole (904) and spacing arc board (905), a slide section of thick bamboo (901) fixed mounting is in placing seat (1), and the quantity of a slide section of thick bamboo (901) is a plurality of, spacing sliding ring (902) sliding connection is in a slide section of thick bamboo (901), reset spring (903) are installed between a slide section of thick bamboo (901) and spacing sliding ring (902), spacing pole (904) fixed connection is on spacing sliding ring (902), spacing arc board (905) fixed mounting is kept away from the one end of spacing sliding ring (902) in spacing pole (904), and spacing arc board (905) and ovum culture dish seat (201) cooperation use.
4. An ovum maturation culture dish according to claim 1, wherein: holding grooves (5) are formed in two sides of the surface of the placement seat (1), two support seats (3) are fixedly arranged at the bottom of the placement seat (1), and anti-slip lines are formed in the bottom of the support seats (3).
5. An ovum maturation culture dish according to claim 1, wherein: the edge of the holding ring (202) is provided with wavy easy-to-hold lines, the number of the clamping strips (217) and the clamping grooves (218) is multiple, and the clamping strips (217) and the clamping grooves (218) are distributed at equal intervals.
6. An ovum maturation culture dish according to claim 1, wherein: one side of the surface of the placement seat (1) is provided with a label labeling area (4), the label labeling area (4) is of a frosted label labeling area, and the label labeling area (4) and the serial number labeling area (205) are of the same type.
7. An ovum maturation culture dish according to claim 1, wherein: the azimuth mark (209) is a digital azimuth mark, the number of the azimuth mark is 3, 6, 9 and 12, and the two sides of the surface of the separation measuring plate (216) are provided with culture oil scales.
8. An ovum maturation culture dish according to claim 1, wherein: the height of the dish cover skirt edge (208) is 2mm, the dish cover skirt edge (208) is matched with the ovum culture dish base (201), and the stacking groove (206) is matched with the bottom of the stacking bottom plate (210).
9. An ovum maturation culture dish according to claim 1, wherein: the surfaces of the four placing grooves (6) are fixedly provided with limit rings (8), the limit rings (8) are matched with stacking convex rings (211), and the stacking convex rings (211) are matched with limit strips (207).
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795765A (en) * | 2018-09-13 | 2018-11-13 | 郑州杏坛电子科技有限公司 | The adjustable culture dish for biological medicine |
| CN111961590A (en) * | 2020-09-11 | 2020-11-20 | 力盟生命科技(深圳)有限公司 | Ovum maturity culture dish |
| CN112970461A (en) * | 2020-12-24 | 2021-06-18 | 安徽大别山浩野魔芋科技开发有限公司 | Cultivation device for konjak planting |
| CN114854586A (en) * | 2022-05-11 | 2022-08-05 | 南昌大学抚州医学院 | Test tube baby culture dish |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP4724854B2 (en) * | 2009-02-09 | 2011-07-13 | 大日本印刷株式会社 | Cell culture vessel |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795765A (en) * | 2018-09-13 | 2018-11-13 | 郑州杏坛电子科技有限公司 | The adjustable culture dish for biological medicine |
| CN111961590A (en) * | 2020-09-11 | 2020-11-20 | 力盟生命科技(深圳)有限公司 | Ovum maturity culture dish |
| CN112970461A (en) * | 2020-12-24 | 2021-06-18 | 安徽大别山浩野魔芋科技开发有限公司 | Cultivation device for konjak planting |
| CN114854586A (en) * | 2022-05-11 | 2022-08-05 | 南昌大学抚州医学院 | Test tube baby culture dish |
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