CN116114693A - Mud flat saline-alkali soil rice growth promoter and preparation method thereof - Google Patents
Mud flat saline-alkali soil rice growth promoter and preparation method thereof Download PDFInfo
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Abstract
The application relates to the technical field of crop growth promotion, and particularly discloses a paddy rice growth promoter for a tidal flat saline-alkali soil. The paddy rice growth promoter for the tidal flat saline-alkali soil comprises the following components in parts by weight: 20-50 parts of biological humic acid, 10-30 parts of biomass carbon, 10-30 parts of citric acid gypsum, 10-20 parts of phosphate, 5-10 parts of modified k-carrageenan, 0.5-2.0 parts of amino acid, 0.005-0.05 part of plant growth regulator, 0.5-2.0 parts of trace elements and 0.05-0.1 part of functional microbial inoculum; wherein, the modified k-carrageenan is obtained by modifying k-carrageenan by microwave, and the preparation method comprises the following steps: and (3) placing the k-carrageenan into microwave equipment, and irradiating for 2-8min under the irradiation power of 300-800W. The paddy rice growth promoter for the tidal flat saline-alkali soil can not only improve and fertilize the tidal flat saline-alkali soil, but also improve the stress resistance and the saline-alkali resistance of paddy rice, and has remarkable promoting effect on the growth of paddy rice.
Description
Technical Field
The application relates to the technical field of crop growth promotion, in particular to a paddy rice growth promoter for a tidal flat saline-alkali soil and a preparation method thereof.
Background
The physiological characteristics of the rice make the rice become the first choice grain crop for development and utilization of coastal beach saline-alkali soil, and a larger value space is provided for planting after the beach saline-alkali soil is improved. The improvement and utilization of the beach saline-alkali soil are divided into two stages, wherein the first stage is engineering improvement: namely, salt is discharged and alkali is reduced by engineering means such as applying a saline-alkali soil modifier, physical and chemical properties of soil are improved, and further the saline-alkali hazard effect is lightened, and the severe saline-alkali soil is gradually improved into mild saline-alkali soil, so that the tidal flat saline-alkali soil has cultivation value; the second stage is planting improvement: the method is to plant crops on the slightly saline-alkali soil with improved engineering, and gradually form a plow layer on the slightly saline-alkali soil through crop planting, so that salt returning of the saline-alkali soil is prevented, and soil fertility is gradually improved.
However, compared with normal soil, the engineering improved beach saline-alkali soil still has the characteristics of 'two-high-one-low', namely high salt content, high pH and low organic matter content of the soil; in addition, the soil environment with heavy soil, poor air permeability and poor microecology also limits the normal growth and yield of the rice, especially the emergence rate of the direct seeding rice and the survival rate of the transplanted rice after transplanting, so that the basic rice seedlings are reduced, and the yield of the paddy rice in the tidal flat saline-alkali soil is seriously influenced.
Therefore, improving the salt stress resistance of plants while improving the saline-alkali soil is also an important factor in the agricultural production of the saline-alkali soil. In order to cope with salt stress, plant cells are protected from injury by imparting various salt-resistant mechanisms to plants, including enhancing antioxidant enzyme activity and non-enzyme antioxidant substance content of plants, maintaining metabolic balance of free radicals, and synthesizing osmoregulating substances to regulate osmotic pressure of plants. For example, the related art provides an organic rice seedling water-locking agent which can solve the problems of slow turning green, poor tillering and the like after the rice is transplanted in the saline-alkali soil, but the water-locking agent cannot be applied to direct-seeding rice, and only improves the salt tolerance of the rice, but does not improve the physicochemical properties of the soil in the tidal flat saline-alkali soil.
Therefore, the rice growth promoter can improve the salt and alkali resistance of rice, promote the growth of the rice in the beach saline-alkali soil, and improve and fertilize the beach saline-alkali soil, and is a technical problem which needs to be solved by the technicians in the field.
Disclosure of Invention
In order to improve and fertilize the soil of the tidal flat saline-alkali soil and simultaneously increase the yield of the rice in the tidal flat saline-alkali soil, the application provides a paddy rice growth promoter for the tidal flat saline-alkali soil and a preparation method thereof.
In a first aspect, the present application provides a paddy rice growth promoter for tidal flat saline-alkali soil, which adopts the following technical scheme:
the paddy rice growth promoter for the tidal flat saline-alkali soil comprises the following components in parts by weight: 20-50 parts of biological humic acid, 10-30 parts of biomass carbon, 10-30 parts of citric acid gypsum, 10-20 parts of phosphate, 5-10 parts of modified k-carrageenan, 0.5-2.0 parts of amino acid, 0.005-0.05 part of plant growth regulator, 0.5-2.0 parts of trace elements and 0.05-0.1 part of functional microbial inoculum;
wherein the modified k-carrageenan is obtained by modifying k-carrageenan by microwave.
The paddy rice growth promoting agent for the tidal flat saline-alkali soil is prepared from the components, can not only improve the salt-tolerant alkalinity of paddy rice and promote the growth of paddy rice on the tidal flat saline-alkali soil, but also improve and fertilize the soil of the tidal flat saline-alkali soil, increase the abundance and activity of the soil, and has good use prospect.
In the application, the k-carrageenan is modified by utilizing microwave equipment, so that the obtained modified k-carrageenan can obviously enhance various stress resistance of rice, improve the absorption of the rice to soil nutrients and moisture, increase the chlorophyll content of the rice and improve the quality of the rice. Biological humic acid can promote crop growth and has certain improvement effect on soil. The citric acid gypsum can reduce the alkalinity in the saline-alkali soil and promote the growth of crops. The trace elements can not only keep the activity of the fertilizer in the saline-alkali environment, but also reduce the total salt content of the saline-alkali soil and improve the salt alkalinity of the soil. The biomass carbon can regulate the generation and distribution of plant hormones, improve the salt tolerance of crops and promote the growth of crop seedlings in saline-alkali soil. The amino acid and the plant growth regulator can promote seed germination and root system growth, and improve stress resistance and salt and alkali resistance of rice. The functional microbial inoculum can stimulate the growth of rice root systems and improve the salt tolerance of rice.
In a specific embodiment, the rice growth promoter comprises the following components in parts by weight: 30 parts of biological humic acid, 20 parts of biomass carbon, 20 parts of citric acid gypsum, 15 parts of phosphate, 8 parts of modified k-carrageenan, 1.5 parts of amino acid, 0.02 part of plant growth regulator, 1.5 parts of trace elements and 0.1 part of functional microbial agent.
Preferably, the biological humic acid is an aerobic fermentation product of kitchen waste; the biological humic acid contains 38-42wt% of total humic acid, 35-40wt% of free humic acid and 14-16wt% of water-soluble humic acid.
The biological humic acid adopted in the application is prepared by aerobic fermentation of the kitchen waste, the source of the kitchen waste is rich and continuous, and the humic acid with high quality, standardization and cost performance can be stably produced. The humic acid content of the kitchen waste aerobic fermentation product is high, so that the growth of crops can be promoted, and the soil can be improved to a certain extent; the pH of the biological humic acid is in the range of 3.5-5.5, and the biological humic acid has strong buffering property, so that the biological humic acid is very suitable for saline-alkali soil; in addition, the biological humic acid is rich in biological stimulations such as small peptide, amino acid, fungus polysaccharide, vitamin and the like, and has the characteristics of improving stress resistance and promoting growth of crops; the biological humic acid also contains protein, easily-oxidized organic matters and crude oil, can be directly used as a carbon nitrogen source of soil microorganisms, increases the types and abundance of microorganisms, and remodels rhizosphere microbial communities of saline-alkali soil.
Preferably, the preparation method of the modified k-carrageenan comprises the following steps: and (3) placing the k-carrageenan into microwave equipment, and irradiating for 2-8min under the irradiation power of 300-800W to obtain the modified k-carrageenan.
In the application, through modifying k-carrageenan, the molecular weight of k-carrageenan can be reduced, active groups are increased, and the activity of the carrageenan is enhanced. The mud flat saline-alkali soil rice growth promoting agent prepared by using the modified k-carrageenan can stimulate the systemic of defensive enzymes in rice, and can enhance various stress resistance of rice such as drought resistance, cold resistance, saline-alkali resistance and the like on one hand; secondly, the root system development of the rice can be promoted, and the absorption and utilization rate of the rice to soil nutrients and moisture can be improved; the three aspects can also increase the chlorophyll content of the rice, improve the photosynthetic efficiency of the rice, greatly improve the yield of the rice and improve the quality of the rice. In the preparation process of the modified k-carrageenan, when the irradiation power is too low and the irradiation time is too short, the modification effect of the k-carrageenan is poor, the molecular weight is large, and the promotion effect on all aspects of rice is small; when the irradiation power is too high and the irradiation time is too long, the k-carrageenan can be gelatinized, and the activity is reduced. Therefore, the irradiation power and irradiation time of the modification process of the k-carrageenan are controlled within the above range, the k-carrageenan can be fully modified and can not be inactivated, and further the promotion effect on stress resistance, yield and quality yield of rice can be good.
Preferably, the phosphate is a mixture of orthophosphate and polyphosphate; the weight ratio of the orthophosphate to the polyphosphate is (2.5-3.5): 1.
preferably, the orthophosphate is selected from the group consisting of calcium superphosphate, monoammonium phosphate and monopotassium phosphate.
Preferably, the polyphosphate has a degree of polymerization n of from 10 to 20.
In the application, the phosphate is a mixture of orthophosphate and polyphosphate, and the orthophosphate can reduce the pH of rhizosphere soil, reduce the fixation of phosphate radicals and promote the growth of seedling root systems; the polyphosphate has good solubility, is not easy to react with calcium, magnesium and other ions in the soil solution to cause phosphate to fail, effectively avoids the fixation of phosphorus by the soil, increases the moving distance of phosphorus in the soil, and nourishes deep root systems; and the polyphosphate can promote the absorption of trace elements such as zinc, boron and the like by crops, and improve the stress resistance of the crops. Therefore, the weight ratio of orthophosphate to polyphosphate is controlled in the range, phosphorus can be prevented from being fixed by soil, and crops can be guaranteed to absorb enough phosphorus, so that the root systems of the crops can grow normally.
In a specific embodiment, the orthophosphate is a mixture of calcium superphosphate, monoammonium phosphate, and monopotassium phosphate; the weight ratio of the calcium superphosphate to the monoammonium phosphate to the monopotassium phosphate is 3:2:1.
in some embodiments, the weight ratio of the orthophosphate to the polyphosphate may be (2-2.5): 1. (2-3): 1. (2-3.5): 1. (2-4): 1. (2.5-3): 1. (2.5-4): 1. (3-3.5): 1. (3-4): 1 or (3.5-4): 1.
in a specific embodiment, the weight ratio of the orthophosphate to the polyphosphate may also be 2:1. 2.5:1. 3: 1. 3.5:1 or 4:1.
preferably, the amino acid is selected from at least 4 of polyaspartic acid, polyglutamic acid, glutamic acid, arginine, methionine and tetramethylglutaric acid; the plant growth regulator is at least 4 selected from Gibberellic Acid (GA), alpha-sodium naphthalene acetate, indolebutyric acid (IBA), salicylic acid (BHA) and 6-glycosyl amino purine (KT).
In the application, the amino acid and the plant growth regulator can promote seed germination and root system growth, and improve stress resistance and salt and alkali resistance of rice. Wherein, the plant regulating agent has quick action effect and short lasting period; while amino acids have a relatively long duration and are milder. Therefore, the application combines the amino acid and the plant growth regulator according to a specific proportion, and the action effect on rice is more outstanding and durable. Alpha-sodium naphthalene acetate and indolebutyric acid (IBA) can promote the growth of main roots and fibrous roots of rice; gibberellic Acid (GA) and 6-glycosyl aminopurine (KT) can increase the activity of rice root systems and improve the capability of rice for taking nutrient substances. The polyaspartic acid not only has the effect of promoting the growth of crops, but also can be used as a protective agent to reduce the loss of each effective component in a saline-alkali environment; polyglutamic acid, arginine and methionine can improve the salt tolerance of the rice root system in a saline-alkali environment and promote the growth of the rice root system.
In a specific embodiment, the amino acids are present in a weight ratio of 2:1:2:2, polyaspartic acid, polyglutamic acid, arginine and methionine.
In a specific embodiment, the amino acids are present in a weight ratio of 2:1:2, polyaspartic acid, polyglutamic acid, glutamic acid.
In a specific embodiment, the plant growth regulator is a mixture of 1:2:4:2 are Gibberellic Acid (GA), sodium alpha-naphthylacetate, indolebutyric acid (IBA) and 6-glycosylaminopurine (KT)
Preferably, the trace elements are a mixture of chelated trace elements and sulfate trace elements; the chelate trace elements are selected from EDTA-Zn, EDTA-Fe, EDTA-Cu and sugar alcohol boron, and the sulfate trace elements are selected from copper sulfate, ferrous sulfate, zinc sulfate and manganese sulfate.
In the application, the chelated trace elements can keep the activity of the fertilizer in a saline-alkali environment, so that the loss is avoided; the sulfate microelements can provide microelements for rice, and the substitution capacity of di-and trivalent metal cations in the sulfate microelements is larger than the ion substitution capacity of salt ions sodium ions and potassium ions, so that under the action of polyaspartic acid, the leaching of the salt ions into a soil layer along with irrigation water is facilitated, the total salt content of the saline-alkali soil is reduced, the pH value of a soil solution is reduced, and the saline-alkali property of the soil is improved.
Preferably, the weight ratio of the chelated trace element to the sulfate trace element is (1.5-2.5): 1.
in some embodiments, the weight ratio of the chelated trace element to the sulfate trace element may be (1-1.5): 1. (1-2): 1. (1-2.5): 1. (1.5-2): 1 or (2-2.5): 1.
in a specific embodiment, the weight ratio of the chelating trace element to the sulfate trace element may also be 1: 1. 1.5: 1. 2:1 or 2.5:1.
preferably, the citric acid gypsum comprises CaSO 4 .2H 2 98-99wt% of O, 0.02-0.05wt% of citric acid and 0.5-1.5wt% of trace organic matters (protein and grease); the pH value of the citric acid gypsum is 4.5-6.5.
The citric acid gypsum adopted in the application is a byproduct of producing citric acid, ca in the citric acid gypsum 2+ Can act with sodium carbonate and sodium bicarbonate in the saline-alkali soil, and replace sodium ions with calcium ions to generate sodium sulfate which is easy to dissolve in water, so that salt washing can be realized through irrigation; in addition, the citric acid gypsum contains trace citric acid and organic matters, so that the alkalinity in the saline-alkali soil can be reduced, and the crop growth can be promoted.
In the application, biomass carbon is a porous solid particulate matter rich in carbon prepared from biomass through a high-temperature hydrothermal carbonization method under an anoxic condition. Mixing biomass carbon with citric acid gypsum, and trace amount of organic acid and Ca in the citric acid gypsum 2+ Can activate potassium element and promote Na + Leaching to raise the K/Na ratio in plant and regulate plant excitationAnd the generation and distribution of the element can improve the salt tolerance of crops and promote the growth of crop seedlings in saline-alkali soil.
Preferably, the functional microbial agent is selected from at least two of bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and saccharomycetes.
The saccharomycete has the advantages of strong viability, strong reproductive capacity and strong adaptability, and can utilize amino acid, sugar and other organic substances contained in biological humic acid to generate active substances for promoting cell division through fermentation, so that important nutrition guarantee is provided for the production of matrixes (foods) required by the proliferation of other functional bacteria; bacillus subtilis, bacillus megaterium and bacillus mucilaginosus propagate in soil for growth and metabolism, and can produce various physiologically active substances such as secreted mycin, indoleacetic acid, cytokinin, biological enzyme, aminopolysaccharide substances, proteins and the like, so that the growth of rice root systems can be stimulated, and the salt tolerance of rice can be improved.
Further preferably, the functional microbial inoculum is a composite microbial inoculum containing bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and saccharomycetes; the weight ratio of the bacillus subtilis to the bacillus megaterium to the bacillus mucilaginosus to the saccharomycetes is (1.8-2.2): (1.8-2.2): (0.8-1.2): 1.
in the application, the composite microbial inoculum of bacillus subtilis, bacillus megatherium, bacillus mucilaginosus and saccharomycetes is used as a functional microbial inoculum, and the easily-oxidized organic matters of the biological humic acid can be fully utilized as bait, so that the rapid propagation of strains is realized. The method can promote the survival and the reproductive capacity of functional bacteria of bacillus subtilis, bacillus megatherium and bacillus mucilaginosus through the reproduction and metabolism of saccharomycetes, can secrete crop growth hormone and bioactive substances in the metabolic process of the functional bacteria of bacillus subtilis, bacillus megatherium and bacillus mucilaginosus, and can activate nutrient substances in saline-alkali soil, improve the saline-alkali stress resistance of rice and promote the growth of rice root systems. The weight ratio of the bacillus subtilis, the bacillus megaterium, the bacillus mucilaginosus and the saccharomycetes is further controlled in the range, and the synergistic use effect of 4 bacterial agents can be optimized, so that the prepared rice growth promoter has the optimal growth promoting effect on rice and the optimal activation effect on saline-alkali soil.
In a specific embodiment, the functional microbial agent is a microbial agent with a weight ratio of 2:2:1:1, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and saccharomycetes.
Preferably, in the composite microbial inoculum, the effective viable count of the bacillus subtilis and the bacillus megaterium is 500 hundred million/g, the effective viable count of the bacillus mucilaginosus is 100 hundred million/g, and the effective viable count of the saccharomycetes is 100 hundred million/g.
In a second aspect, the application provides a preparation method of a paddy rice growth promoter for tidal flat saline-alkali soil.
A preparation method of a paddy rice growth promoter for a tidal flat saline-alkali soil comprises the following steps:
(1) Grinding the components to a particle size of 0.2-2.5mm;
(2) Adding amino acid, plant growth regulator and microelements into a reactor, and stirring for 3-10min at 150-250r/min to obtain a mixture A;
(3) Adding biological humic acid into the mixture A, and stirring for 3-10min at 150-250r/min to obtain a mixture B;
(4) And adding biomass carbon, citric acid gypsum, phosphate and modified k-carrageenan into the mixture B, and stirring for 5-15min at 350-450r/min to obtain the mud flat saline-alkali soil rice growth promoter.
In summary, the present application has the following beneficial effects:
1. the application provides a rice growth promoting agent prepared from biological humic acid, biomass carbon, citric acid gypsum, phosphate, modified k-carrageenan, amino acid, plant growth regulator, trace elements and functional microbial agents, wherein the rice growth promoting agent adopts the modified k-carrageenan obtained by microwave modification, so that salt and alkali resistance of rice can be improved, the growth of the rice on the tidal flat saline-alkali soil can be promoted, soil of the tidal flat saline-alkali soil can be improved and fertilized, the abundance and activity of the soil can be increased, and the rice growth promoting agent has good application prospect.
2. The application adopts microwave equipment to modify k-carrageenan, and controls the irradiation power at 300-800W and the irradiation time at 2-8min, and the obtained modified k-carrageenan has small molecular weight and high activity; the mud flat saline-alkali soil rice growth promoting agent prepared by the method can stimulate the defensive enzyme systematicness in rice, and can enhance various stress resistance of rice such as drought resistance, cold resistance, saline-alkali resistance and the like on one hand; secondly, the root system development of the rice can be promoted, and the absorption and utilization rate of the rice to soil nutrients and moisture can be improved; the three aspects can also increase the chlorophyll content of the rice, improve the photosynthetic efficiency of the rice, greatly improve the yield of the rice and improve the quality of the rice.
3. The phosphate adopted in the rice growth promoter is a mixture of orthophosphate and polyphosphate, and the weight ratio of the orthophosphate to the polyphosphate is (2.5-3.5): 1, the rice growth promoter prepared by the phosphate can reduce the fixation of phosphate in soil, increase the absorption of crops to phosphorus, and further promote the growth of rice seedling root systems.
4. The trace elements adopted in the application are a mixture of chelated trace elements and sulfate trace elements, and the weight ratio of the chelated trace elements to the sulfate trace elements is controlled to be (1.5-2.5): within the range of 1, the fertilizer can maintain activity in a saline-alkali environment, can reduce the total salt content in the saline-alkali soil, reduce the pH value of a soil solution and improve the salinization of the soil.
Drawings
Fig. 1 is a flowchart of a preparation method of the mud flat saline-alkali soil rice growth promoter provided by the application.
Detailed Description
The application provides a paddy rice growth promoter for a tidal flat saline-alkali soil, which comprises the following components in parts by weight: 20-50 parts of biological humic acid, 10-30 parts of biomass carbon, 10-30 parts of citric acid gypsum, 10-20 parts of phosphate, 5-10 parts of modified k-carrageenan, 0.5-2.0 parts of amino acid, 0.005-0.05 part of plant growth regulator, 0.5-2.0 parts of trace elements and 0.05-0.1 part of functional microbial inoculum.
Wherein the phosphate is a mixture of orthophosphate and polyphosphate; the weight ratio of the orthophosphate to the polyphosphate is (2-4): 1, a step of; orthophosphate is selected from calcium superphosphate, monoammonium phosphate and monopotassium phosphate, and the polymerization degree n of the polyphosphate is 10-20. The plant growth regulator is prepared from the following components in percentage by weight: 2:4:2 are Gibberellic Acid (GA), sodium alpha-naphthylacetate, indolebutyric acid (IBA) and 6-glycosyl aminopurine (KT). The amino acid is at least 4 selected from polyaspartic acid, polyglutamic acid, glutamic acid, arginine, methionine and tetramethyl glutaric acid. The microelements are a mixture of chelated microelements and sulfate microelements; the chelating trace elements are selected from EDTA-Zn, EDTA-Fe, EDTA-Cu and sugar alcohol boron, and the sulfate trace elements are selected from copper sulfate, ferrous sulfate, zinc sulfate and manganese sulfate. The functional bacterial agent is selected from at least two of bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and saccharomycetes.
The preparation method of the modified k-carrageenan comprises the following steps: and (3) placing the k-carrageenan into microwave equipment, and irradiating for 2-8min under the irradiation power of 300-800W to obtain the modified k-carrageenan.
The preparation method of the mud flat saline-alkali soil rice growth promoter comprises the following steps:
(1) Grinding the components to a particle size of 0.2-2.5mm;
(2) Adding amino acid, plant growth regulator and microelements into a reactor, and stirring for 3-10min at 150-250r/min to obtain a mixture A;
(3) Adding biological humic acid into the mixture A, and stirring for 3-10min at 150-250r/min to obtain a mixture B;
(4) And adding biomass carbon, citric acid gypsum, phosphate and modified k-carrageenan into the mixture B, and stirring for 5-15min at 350-450r/min to obtain the mud flat saline-alkali soil rice growth promoter.
In the specific embodiment of the application, the biological humic acid is purchased from Beijing Jiabo biotechnology Co., ltd, and contains 40wt% of total humic acid, 38wt% of free humic acid, 15wt% of water-soluble humic acid and 5% of other bioactive substances; biomass carbon was purchased from Jiangsu Huafeng agricultural bioengineering limited; the citric acid gypsum contains CaSO 4 .2H 2 98.5wt% of O, 0.04wt% of citric acid, 1.1wt% of trace organic matters (protein and grease), and the pH value of the citric acid gypsum is 6; the CAS number of the ammonium polyphosphate is 14728-39-3, and the polymerization degree is 18; the CAS number of k-carrageenan is 11114-20-8, and is purchased from Jiangsu Aofu biotechnology Co., ltd; bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and yeast are all purchased from beijing jaboticaba biotechnology limited; the remaining materials, reagents, solvents, and the like, are also commercially available in this application.
The present application is described in further detail below in conjunction with the preparation examples, illustrations and performance test.
Preparation example
Preparation example 1
Preparation example 1 provides a modified k-carrageenan.
The preparation method of the modified k-carrageenan comprises the following steps: weighing 10g of k-carrageenan in a culture dish, placing the culture dish in a microwave oven, irradiating for 5min under irradiation power of 500W, and naturally cooling to normal temperature to obtain the modified k-carrageenan.
PREPARATION EXAMPLES 2 to 5
Preparation examples 2-5 each provide a modified k-carrageenan.
Preparation examples 2 to 5 were carried out in accordance with the method of preparation example 1, except that: the irradiation conditions of k-carrageenan are shown in Table 1.
TABLE 1 irradiation conditions in preparation examples 2-5
Comparative preparation example 1
Comparative preparation 1 provides a modified k-carrageenan.
Comparative preparation 1 was conducted in accordance with the method of preparation 1 except that: the irradiation power of k-carrageenan was 200W.
Comparative preparation example 2
Comparative preparation 2 provides a modified k-carrageenan.
Comparative preparation 2 was conducted in accordance with the method of preparation 1, except that: the irradiation power of k-carrageenan was 1000W. During the preparation, it was found that the above-mentioned k-carrageenan had already been gelatinized.
Examples
Example 1
Example 1 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The mud flat saline-alkali soil rice growth promoter comprises the following components in parts by weight: 30g of biological humic acid, 20g of biomass carbon, 20g of citric acid gypsum, 15g of phosphate, 8g of modified k-carrageenan, 1.5g of amino acid, 0.02g of plant growth regulator, 1.5g of trace elements and 0.1g of functional microbial inoculum.
The weight ratio of phosphate is 3:2:1:2, monoammonium phosphate, monopotassium phosphate and ammonium polyphosphate; modified k-carrageenan was derived from preparation 1; the weight ratio of the amino acid is 2:1:2:2 polyaspartic acid, polyglutamic acid, arginine and methionine; the plant growth regulator is prepared from the following components in percentage by weight: 2:4:2 are Gibberellic Acid (GA), sodium alpha-naphthylacetate, indolebutyric acid (IBA) and 6-glycosyl aminopurine (KT); the weight ratio of the microelements is 2:2:1:1 EDTA-Zn, boric sugar alcohol, ferrous sulfate and zinc sulfate; the weight ratio of the functional microbial inoculum is 2:2:1:1, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and saccharomycetes.
The preparation method of the mud flat saline-alkali soil rice growth promoter specifically comprises the following steps:
(1) Grinding the components to a particle size of 0.2-2.5mm;
(2) Adding amino acid, a plant growth regulator and trace elements into a reactor, and stirring for 5min at 200r/min to obtain a mixture A;
(3) Adding biological humic acid into the mixture A, and stirring for 5min at 200r/min to obtain a mixture B;
(4) And adding biomass carbon, citric acid gypsum, phosphate and modified k-carrageenan into the mixture B, and stirring for 10min at 400r/min to obtain the mud flat saline-alkali soil rice growth promoter.
Examples 2 to 8
Examples 2-8 respectively provide a paddy rice growth promoter for tidal flat saline-alkali soil.
The above embodiment differs from embodiment 1 in that: phosphate. The phosphates in examples 2 to 8 were prepared according to Table 2, and the amounts of the phosphates in the mud flat saline-alkali soil rice growth promoters provided in examples 2 to 8 were 15g.
Table 2 Components and addition amounts of phosphate in the mud flat saline-alkali soil rice growth promoting agent provided in examples 2 to 8
Example 9
Example 9 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above embodiment differs from embodiment 1 in that: the amino acid weight ratio in example 9 is 2:1:2, polyaspartic acid, polyglutamic acid, glutamic acid.
Examples 10 to 16
Examples 10-16 provide a paddy rice growth promoter for tidal flat saline-alkali soil.
The above embodiment differs from embodiment 1 in that: the trace elements and the weight ratio of each component are shown in Table 3.
Table 3 microelements in the mud flat saline-alkali soil rice growth promoting agent provided in examples 10 to 16
Example 17
Example 17 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above embodiment differs from embodiment 1 in that: the functional microbial inoculum in the embodiment 17 is a composite microbial inoculum containing bacillus subtilis and saccharomycetes; the weight ratio of the bacillus subtilis to the saccharomycetes is 2:1.
example 18
Example 18 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above embodiment differs from embodiment 1 in that: the functional microbial inoculum in the embodiment 18 is a composite microbial inoculum containing bacillus subtilis and bacillus mucilaginosus; the weight ratio of the bacillus subtilis to the bacillus mucilaginosus is 2:1.
examples 19 to 22
Examples 19-22 respectively provide a paddy rice growth promoter for tidal flat saline-alkali soil.
The above embodiment differs from embodiment 1 in that: the k-carrageenan in examples 19-22 was derived from preparation examples 2-5, respectively.
Comparative example
Comparative example 1
Comparative example 1 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above comparative example is different from example 1 in that: and replacing the modified k-carrageenan with k-carrageenan.
Comparative example 2
Comparative example 2 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above comparative example is different from example 1 in that: the mud flat saline-alkali soil rice growth promoter provided in comparative example 2 is free of biomass carbon.
Comparative example 3
Comparative example 3 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above comparative example is different from example 1 in that: the biological humic acid is replaced by coal-based humic acid.
Comparative example 4
Comparative example 4 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above comparative example is different from example 1 in that: and replacing the citric acid gypsum with phosphogypsum.
Comparative example 5
Comparative example 5 provides a paddy rice growth promoter for tidal flat saline-alkali soil.
The above comparative example is different from example 1 in that: the addition amount of each component in the paddy rice growth promoter in the tidal flat saline-alkali soil.
The mud flat saline-alkali soil rice growth promoter provided in comparative example 5 comprises the following components in percentage by weight: 60g of biological humic acid, 40g of biomass carbon, 20g of citric acid gypsum, 5g of phosphate, 15g of modified k-carrageenan, 1.5g of amino acid, 0.02g of plant growth regulator, 1.5g of trace elements and 0.1g of functional microbial inoculum.
Comparative examples 6 to 7
Comparative examples 6 to 7 provide a paddy rice growth promoter for tidal flat saline-alkali soil, respectively.
The above comparative example is different from example 1 in that: the k-carrageenan in comparative examples 6-7 was derived from comparative preparation examples 1-2, respectively.
Performance test
Soil physical and chemical property detection
The performance of the mud flat saline-alkali soil rice growth promoting agent provided in examples 1 to 22 and comparative examples 1 to 7 of the present application was tested, and the test method was as follows:
1. test site: the test field is positioned in a Shangyang division company farm of a Shangyang coastal group of Jiangsu county, the pH value of the soil in the region is 8.66, the basicity is 11%, and the soil belongs to moderate and mild saline-alkali soil.
2. The test method comprises the following steps: dividing 24 test fields in the test fields, wherein the area of each test field is 4m 2 Then the rice growth promoters provided in examples 1 to 18 and comparative examples 1 to 5 were applied to the above-mentioned test fields at an application rate of 0.5kg/m, respectively 2 1 test field is a blank control group, and no substance is applied; the pH of the soil was tested after two months. Soil total salt amount and soil total alkalization degree.
The pH value detection method of the soil comprises the following steps: NY-T1377-2007;
the method for detecting the salt content of the soil comprises the following steps: NY/T1121.16-2006;
the soil organic matter detection method comprises the following steps: NY/T1121.6-2006.
3. Test results: before the rice growth promoter is applied, the pH value of the soil is 8.66, the total salt content of the soil is 0.21%, and the organic matter of the soil is 0.79%. The physicochemical properties of the soil after application of the rice growth promoter are shown in Table 4.
Table 4 physicochemical properties of soil in 30 test fields
According to the detection results of examples 1-22 and the blank control group in Table 4, the rice growth promoter provided by the application can improve the pH value of the saline-alkali soil, improve the organic matter content in the saline-alkali soil and reduce the total salt content of the soil.
Rice growth test
1. Test site: the test field is located in the double-ocean-zone farm of the Zhuangyang division company of the Zhuansu coastal group of Zhuangyang county, jiangsu province, and the pH value of the soil in the double-ocean-zone farm is 8.67, and the salt content is 0.23%.
2. The test method comprises the following steps:
(1) At 2021, 6 and 5 days, dividing 30 test fields into 4m each 2 Then the rice growth promoters provided in examples 1 to 22 and comparative examples 1 to 7 were applied to 29 test fields at an application rate of 0.5kg/m, respectively 2 And applying a nitrogen-phosphorus-potassium compound fertilizer (NPK: 22-8-10) 50 kg/mu, wherein 1 test field is used as a blank control group, no substances are applied except the nitrogen-phosphorus-potassium compound fertilizer with equal quantity, rotary tillage, irrigation and secondary rotary tillage are performed after fertilization, and then the water surface height is kept to be 3-4cm.
(2) Transplanting rice in the test fields respectively at 2021, 6 and 13 days, and observing the growth condition (survival rate) of rice surfaces and the total root length of the rice in each test field 60 days after transplanting (booting period); the rice was harvested at 25 days of 10 months and subjected to yield test, and the results are shown in Table 5.
TABLE 5 Rice growth test results
According to the detection results of Table 5, the survival rate of the rice cultivated by the rice growth promoting agent provided by examples 1-22 is more than or equal to 90%, the root length is more than or equal to 3000cm, and the yield is more than or equal to 500 kg/mu; comparative example 1 the root length of rice cultivated with the rice growth promoter prepared by k-carrageenan is only 2889.1cm, and the yield is 507.7 kg/mu; comparative example 2 the survival rate of rice cultivated with the rice growth promoter prepared without adding biomass carbon was 89.56%, the root length was 2939.7cm, and the yield was 503.1 kg/mu; comparative example 3 the survival rate of rice cultivated with the rice growth promoter prepared by coal-based humic acid was 96.32%, root length was 2786.5cm only, and yield was 462.5 kg/mu; comparative example 4 Rice plant growth promoter prepared from phosphogypsum the root length of the rice plant cultivated is 2897.6cm only, and the yield is 497.3 kg/mu; the survival rate of the rice cultivated by the rice growth promoter obtained in the comparative example 5 is 85.31%, the root length is only 2670.9cm, and the yield is 451.2 kg/mu; the survival rate of the rice in the blank control group is 83.36%, the root length is only 2374.5cm, and the yield is 421.3 kg/mu. Therefore, the rice growth promoter provided by the embodiment of the application can obviously improve the survival rate of rice, promote the growth of rice root systems and further improve the yield of rice.
From the results of the tests in examples 1 to 8, the rice growth promoting agents obtained in examples 1 and 5 to 8 had better growth promoting effect on rice than those in examples 2 to 4, which means that calcium superphosphate, monoammonium phosphate and monopotassium phosphate were used as orthophosphate in the present application, and the weight ratio of orthophosphate to polyphosphate was controlled to be (2.5 to 3.5): in the range of 1, the obtained rice growth promoter has better growth promoting effect on rice.
As can be seen from the detection results of example 1 and example 9, the rice root length of example 9 is slightly shorter than that of example 10, which shows that the rice growth promoting agent obtained by using four amino acids of polyaspartic acid, polyglutamic acid, arginine and methionine in combination has better growth promoting effect on the rice root system.
According to the detection results of the embodiment 1 and the embodiment 10-16, the rice survival rate and the yield in the embodiment 1 and the embodiment 12-15 are slightly better than those in the embodiment 10-11; further, the rice root length of the rice in example 1 and example 13 was found to be not less than 3000cm, which indicates that the weight ratio of the chelate trace element to the sulfate trace element was controlled to be (1.5-2.5): in the range of 1, the growth of the root system of the rice can be further promoted.
According to the detection results of the embodiment 1 and the embodiment 17-18, the survival rate of the rice of the embodiment 1 is 95.65%, the root length is 3421.9cm, and the yield is 548.2 kg/mu; whereas the rice of examples 17-18 had a survival rate of 91.98-92.37%, a root length of 3126.7-3209.2cm and a yield of 527.5-528.1 kg/mu. The rice growth promoting agent prepared by the composite microbial inoculum of bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and saccharomycetes has better growth promoting effect on rice.
According to the detection results of the embodiment 1, the embodiment 19-22 and the comparative example 6-7, the survival rate, the root length and the yield of the rice in the embodiment 1 and the embodiment 19-22 are better than those of the rice in the comparative example 6-7, which shows that the irradiation power in the preparation process of the modified k-carrageenan is controlled to be 300-800W and the time is controlled to be 2-8min, so that the k-carrageenan can be ensured to be completely modified, the k-carrageenan can be ensured not to be gelatinized and the basic activity can be maintained, and further, the root development and the yield of the rice can be well promoted.
While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (10)
1. The paddy rice growth promoter for the tidal flat saline-alkali soil is characterized by comprising the following components in parts by weight: 20-50 parts of biological humic acid, 10-30 parts of biomass carbon, 10-30 parts of citric acid gypsum, 10-20 parts of phosphate, 5-10 parts of modified k-carrageenan, 0.5-2.0 parts of amino acid, 0.005-0.05 part of plant growth regulator, 0.5-2.0 parts of trace elements and 0.05-0.1 part of functional microbial inoculum;
wherein the modified k-carrageenan is obtained by modifying k-carrageenan by microwave.
2. The mud flat saline-alkali soil rice growth promoter according to claim 1, wherein the preparation method of the modified k-carrageenan is as follows: and (3) placing the k-carrageenan into microwave equipment, and irradiating for 2-8min under the irradiation power of 300-800W to obtain the modified k-carrageenan.
3. The mud flat saline-alkali soil rice growth promoter according to claim 1, wherein the phosphate is a mixture of orthophosphate and polyphosphate; the weight ratio of the orthophosphate to the polyphosphate is (2.5-3.5): 1.
4. a beach saline land rice growth promoting agent as claimed in claim 3, wherein the orthophosphate is selected from the group consisting of calcium superphosphate, monoammonium phosphate and monopotassium phosphate.
5. The mud flat saline-alkali soil rice growth promoting agent according to any one of claims 1 to 4, wherein the amino acids are at least 4 selected from the group consisting of polyaspartic acid, polyglutamic acid, glutamic acid, arginine, methionine and tetramethylglutaric acid; the plant growth regulator is at least 4 selected from Gibberellic Acid (GA), alpha-sodium naphthalene acetate, indolebutyric acid (IBA), salicylic acid (BHA) and 6-glycosyl amino purine (KT).
6. The mud flat saline-alkali soil rice growth promoting agent according to any one of claims 1 to 4, wherein the trace elements are a mixture of chelated trace elements and sulfate trace elements; the chelate trace elements are selected from EDTA-Zn, EDTA-Fe, EDTA-Cu and sugar alcohol boron, and the sulfate trace elements are selected from copper sulfate, ferrous sulfate, zinc sulfate and manganese sulfate.
7. The mud flat saline-alkali soil rice growth promoting agent according to claim 6, wherein the weight ratio of the chelate-state trace elements to the sulfate-state trace elements is (1.5-2.5): 1.
8. the mud flat saline-alkali soil rice growth promoting agent according to any one of claims 1 to 4, wherein the functional microbial agent is at least two selected from the group consisting of bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and yeast.
9. The mud flat saline-alkali soil rice growth promoting agent according to claim 8, wherein the functional microbial agent is a composite microbial agent containing bacillus subtilis, bacillus megaterium, bacillus mucilaginosus and saccharomycetes; the weight ratio of the bacillus subtilis to the bacillus megaterium to the bacillus mucilaginosus to the saccharomycetes is (1.8-2.2): (1.8-2.2): (0.8-1.2): 1.
10. the method for preparing the mud flat saline-alkali soil rice growth promoter according to any one of claims 1 to 9, comprising the following steps:
(1) Grinding the components to a particle size of 0.2-2.5mm;
(2) Adding amino acid, plant growth regulator and microelements into a reactor, and stirring for 3-10min at 150-250r/min to obtain a mixture A;
(3) Adding biological humic acid into the mixture A, and stirring for 3-10min at 150-250r/min to obtain a mixture B;
(4) And adding biomass carbon, citric acid gypsum, phosphate and modified k-carrageenan into the mixture B, and stirring for 5-15min at 350-450r/min to obtain the mud flat saline-alkali soil rice growth promoter.
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