CN116113693A8 - 用于胚胎干细胞扩增的组合物和方法 - Google Patents

用于胚胎干细胞扩增的组合物和方法 Download PDF

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Publication number
CN116113693A8
CN116113693A8 CN202180056020.9A CN202180056020A CN116113693A8 CN 116113693 A8 CN116113693 A8 CN 116113693A8 CN 202180056020 A CN202180056020 A CN 202180056020A CN 116113693 A8 CN116113693 A8 CN 116113693A8
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compositions
methods
embryonic stem
stem cell
cell expansion
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Pending
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CN202180056020.9A
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CN116113693A (zh
Inventor
里拉驰·阿隆
拉米·斯卡利特尔
拉维德·提科茨基
达纳·哈尤恩尼曼
奥费尔·韦瑟尔
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Lineage Cell Therapeutics Inc
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Lineage Cell Therapeutics Inc
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Application filed by Lineage Cell Therapeutics Inc filed Critical Lineage Cell Therapeutics Inc
Publication of CN116113693A publication Critical patent/CN116113693A/zh
Publication of CN116113693A8 publication Critical patent/CN116113693A8/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

本文提供了使用包括微载体的可悬浮的扩增复合物扩增人胚胎干的方法和组合物。
CN202180056020.9A 2020-08-10 2021-08-10 用于胚胎干细胞扩增的组合物和方法 Pending CN116113693A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063063942P 2020-08-10 2020-08-10
US63/063,942 2020-08-10
PCT/US2021/045437 WO2022035895A1 (en) 2020-08-10 2021-08-10 Compositions and methods for embryonic stem cell expansion

Publications (2)

Publication Number Publication Date
CN116113693A CN116113693A (zh) 2023-05-12
CN116113693A8 true CN116113693A8 (zh) 2023-12-08

Family

ID=80248133

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180056020.9A Pending CN116113693A (zh) 2020-08-10 2021-08-10 用于胚胎干细胞扩增的组合物和方法

Country Status (9)

Country Link
US (1) US20230313127A1 (zh)
EP (1) EP4192949A1 (zh)
JP (1) JP2023537969A (zh)
KR (1) KR20230096968A (zh)
CN (1) CN116113693A (zh)
AU (1) AU2021325890A1 (zh)
CA (1) CA3190791A1 (zh)
IL (1) IL300436A (zh)
WO (1) WO2022035895A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024102777A2 (en) * 2022-11-08 2024-05-16 Lineage Cell Therapeutics, Inc. Compositions and method for expansion of embryonic stem cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9150829B2 (en) * 2009-03-20 2015-10-06 Agency For Science, Technoloy And Research Culture of pluripotent and multipotent cells on microcarriers
JP5590646B2 (ja) * 2009-10-08 2014-09-17 国立大学法人大阪大学 ヒト多能性幹細胞用培養基材およびその利用

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Publication number Publication date
KR20230096968A (ko) 2023-06-30
CA3190791A1 (en) 2022-02-17
EP4192949A1 (en) 2023-06-14
IL300436A (en) 2023-04-01
JP2023537969A (ja) 2023-09-06
CN116113693A (zh) 2023-05-12
US20230313127A1 (en) 2023-10-05
WO2022035895A1 (en) 2022-02-17
AU2021325890A1 (en) 2023-03-09

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SE01 Entry into force of request for substantive examination
CI02 Correction of invention patent application
CI02 Correction of invention patent application

Correction item: Applicant|Address|Applicant

Correct: Lineage cell therapy Co.,Ltd.|California, USA

False: Lineage cell therapy Co.,Ltd.|California, USA|Lilachi Alon|Rami Scalittle|Ravid Tikotsky|Dana Hayouneniman|Orfeld Wessel

Number: 19-02

Page: The title page

Volume: 39

Correction item: Applicant|Address|Applicant

Correct: Lineage cell therapy Co.,Ltd.|California, USA

False: Lineage cell therapy Co.,Ltd.|California, USA|Lilachi Alon|Rami Scalittle|Ravid Tikotsky|Dana Hayouneniman|Orfeld Wessel

Number: 19-02

Volume: 39

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