CN116008574B - Immune group ization all-in-one - Google Patents
Immune group ization all-in-one Download PDFInfo
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- CN116008574B CN116008574B CN202211610285.1A CN202211610285A CN116008574B CN 116008574 B CN116008574 B CN 116008574B CN 202211610285 A CN202211610285 A CN 202211610285A CN 116008574 B CN116008574 B CN 116008574B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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Abstract
The invention belongs to the technical field of medical instruments, and particularly relates to an immunohistochemical integrated machine. Aiming at the problems that the immunohistochemistry is performed mostly manually and wastes time and energy in the prior art, the invention provides an immunohistochemical all-in-one machine, a fixing device for fixing a detection plate is arranged in the machine, the fixing device is connected with a moving mechanism for driving the fixing device to sequentially reach the designated positions of all chambers, the fixing device comprises a fixing mechanism and a cover plate opening mechanism for opening a cover plate of the detection plate, a control system is arranged in the machine, the control system is electrically connected with the moving device, the fixing device and a liquid adding device arranged in all chambers, and the control system is connected with a control button arranged on the outer surface of the machine.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to an immunohistochemical integrated machine.
Background
Immunohistochemistry is a study for determining intracellular antigens (polypeptides and proteins) of tissues by developing color-developing agents (luciferin, enzymes, metal ions, isotopes) of labeled antibodies through chemical reaction by applying an immunological basic principle, namely antigen-antibody reaction, namely the principle of specific binding of antigen and antibody, and carrying out localization, qualitative and relative quantitative research on the antigens (polypeptides and proteins), which is called immunohistochemical technology or immunocytochemical technology.
In the prior art, a tissue slice to be seen is subjected to dewaxing, hydration and antigen restoration, then is subjected to slice preparation, is placed into a plate washer for cleaning after slice preparation, is taken out and dripped with sealing liquid for incubation after cleaning, is cleared away after incubation, is added with primary antibody for re-incubation, is cleaned again after incubation, is dripped with secondary antibody for incubation after cleaning, is cleaned again after incubation, is subjected to color development, is subjected to cleaning, transparency and sealing after color development, and is scanned finally. The process is complicated, and the operation is performed manually at present, so that an immune-histochemical integrated machine is necessary to be designed for carrying out immune-histochemical, so that the artificial work is replaced, and the workload of workers is reduced.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an immune-histochemical integrated machine.
The technical scheme adopted by the invention is as follows:
the integrated machine comprises a sample inlet communicated with the cleaning chamber and a sample outlet communicated with the sealing chamber, liquid adding devices are arranged in the cleaning chamber, the sealing liquid incubating chamber, the first anti-incubating chamber, the second anti-incubating chamber, the color developing chamber, the transparent chamber and the sealing chamber, the liquid adding devices are connected with a waste liquid barrel arranged on the outer surface of the integrated machine, a fixing device used for fixing a detection plate is arranged in the integrated machine, the fixing device is connected with a moving mechanism used for driving the fixing device to sequentially reach the designated positions of the chambers, the fixing device comprises a fixing mechanism and a cover plate opening mechanism used for opening a cover plate of the detection plate, a control system is arranged in the integrated machine and is electrically connected with the moving mechanism, the fixing device and the liquid adding devices arranged on the chambers, and the control system is connected with a control button arranged on the outer surface of the integrated machine.
After the technical scheme is adopted, a detection plate with a sample is placed into a fixing mechanism of a control device from a sample inlet, the fixing mechanism is sequentially moved to each chamber by the moving mechanism according to a set program, for example, after the fixing mechanism is moved to a cleaning chamber and then to a position corresponding to a liquid adding device in the cleaning chamber according to the set program, a control system controls a cover plate opening mechanism to open a cover plate of the detection plate, then cleaning liquid is dripped into the detection plate through the liquid adding device, waste liquid is sucked into a waste liquid barrel through the liquid adding device after the cleaning is finished, after the cleaning is repeated for several times according to the set program, the fixing device after the cleaning is moved to a designated position of a closed liquid incubation chamber by the moving mechanism, sealing liquid is dripped into the detection plate through the liquid adding device of the closed liquid incubation chamber, then the cover plate is covered on the detection plate by the cover plate opening device for incubation, the volatilization of the closed liquid is avoided, after the incubation is finished, the fixing device is moved to the cleaning chamber again, all steps are finished according to the set program, the sealing plate is sealed, and the detection plate is taken out from a sampling port for scanning.
Preferably, the all-in-one machine comprises a matched detection plate and a glass slide, wherein the detection plate comprises a fixed bottom plate, a porous plate and a cover plate, a plurality of second grooves for placing the glass slide are formed in the fixed bottom plate, the fixed bottom plate is detachably connected with the cover plate, the fixed bottom plate is connected with the porous plate in a clamping mode, the front face of the glass slide comprises a marking frosted area and a tissue slice preparation area, a plurality of first sample holes are formed in the tissue slice preparation area, a plurality of second sample holes matched with the first sample holes are formed in the porous plate, and the first sample holes and the second sample holes are arranged in a matrix mode.
After the technical scheme is adopted, the common glass slide only comprises a marked frosting area and a tissue slice preparation area, a tissue slice sample is usually prepared or a plurality of tissue slice samples are prepared in the same area under special conditions, when dyeing (immunohistochemistry or special dyeing) is carried out, the different methods (a histochemical pen and the like) can only be used for partitioning, but only the same sample can be used for carrying out one type of dyeing, the waste is serious when antibodies and dyeing liquid are used, and the dyeing cost is increased. The slide glass is provided with a plurality of sample Kong Ranse areas at the bottom of the slice and is matched with the sample holes on the detection plate, the same sample can be divided into a plurality of areas, and detailed labeling can be carried out according to the sample hole marks, so that the phenomenon of misaddition during the addition of antibodies or staining solution in the staining process is prevented. If the glass slide is used for preparing tissue sections for dyeing and is combined with a detection plate, the simultaneous performance of multiple antibodies or multiple dyeings of the same glass slide and the same sample or multiple dyeings of multiple samples can be realized, and the time required for dyeing is greatly saved. Meanwhile, the use of antibodies or staining solution can be saved to the greatest extent due to small area of the detection sample holes, and the staining cost can also be saved.
Preferably, the fixing mechanism comprises a base, a first groove for placing the detection plate is formed in the base, at least two first telescopic rods are symmetrically arranged on the base, pressing plates are fixedly arranged at the upper ends of the first telescopic rods, protruding portions are symmetrically arranged on two sides of the detection plate, and a pushing mechanism for pushing the detection plate out of the first groove when the fixing device moves the sealing piece chamber is arranged at the bottom of the detection plate.
After the technical scheme is adopted, the detection plate is placed in the first groove, then the first telescopic rod is controlled to shrink, the detection plate is pressed against the first groove by the pressing plate, the detection plate is moved when the cover plate is prevented from being opened by the cover plate opening mechanism, and the detection plate is conveniently taken out by the pushing mechanism.
Preferably, a plurality of drain holes, a plurality of air inlets and a plurality of air outlets are arranged in the first groove, the drain holes are connected with the waste liquid barrel through pipelines, a second liquid discharge pump is arranged in the pipeline between the drain holes and the waste liquid barrel, the plurality of air inlets and the plurality of air outlets are oppositely arranged, the air inlets are connected with a gas purifying device arranged in the integrated machine through pipelines, a gas inlet pump is arranged in the pipeline, the gas inlet pump is electrically connected with a control system, and the air outlets are communicated with an external pipeline.
Preferably, the liquid adding device comprises a fixed base fixedly arranged in each chamber, a plurality of mounting seats are arranged on the fixed base, each mounting seat is connected with the fixed base through at least one second telescopic rod, each mounting seat is provided with a plurality of liquid adding rods, the number of the liquid adding rods on each mounting seat and the number of the mounting seats respectively correspond to the number of rows and the number of columns of the second sample holes on the porous plate, one end of each liquid adding rod is provided with a liquid adding needle and a liquid discharging needle, a liquid adding channel and a liquid discharging channel which are respectively communicated with the liquid adding needle and the liquid discharging needle are arranged in each liquid adding rod, the liquid storing grooves are formed in the fixed base through pipeline connection, the number of the liquid storing grooves corresponds to the number of the liquid adding rods, a flowmeter and a first electric valve are arranged in each liquid adding channel, each liquid discharging channel is connected with a pump body arranged in the mounting seat through a pipeline, each pump body is connected with a waste liquid barrel through a pipeline, and each pump body and each second electric valve is electrically connected with a control system.
Preferably, the top of fixing base is provided with the third recess, the top of all-in-one is provided with the upper cover with third recess intercommunication, the reservoir is matrix form evenly setting in the third recess, the lateral wall of third recess is provided with the several water inlet, the water inlet all links to each other with outside water supply system through the pipeline, be provided with the second electric valve on the pipeline, the second electric valve is connected with the control system electricity.
After the technical scheme is adopted, required reagent can be added into each liquid storage tank after the upper cover is opened, the liquid adding needle and the liquid discharging needle on the liquid adding rod on each mounting seat can reach the detection plate to complete liquid absorption or liquid adding through controlling the second telescopic rod to stretch out and draw back, when the reagent needs to be replaced, the liquid storage tank and the liquid adding rod need to be cleaned, after the cleaning procedure is started, the control system firstly controls the fixing device to reach the room in which the reagent needs to be replaced, then controls the second telescopic rod to stretch out and draw back, the liquid adding needle and the liquid discharging needle on the liquid adding rod reach the first groove, then the second electric valve is opened, water of an external water supply system enters the third groove from the water inlet, then the first drainage pump and the second drainage pump are started, and waste water in the first groove is discharged into the waste liquid barrel until the cleaning is completed.
Preferably, the cover plate opening mechanism comprises a rotating seat fixedly arranged on the fixing mechanism, a third telescopic rod is fixedly connected to the rotating seat, the telescopic direction of the third telescopic rod is perpendicular to the plane where the fixing mechanism is located, a supporting rod is fixedly arranged at the top of the third telescopic rod, the plane where the supporting rod is located is horizontal to the plane where the fixing mechanism is located, the plane where the supporting rod is located above the plane where the fixing mechanism is located, a fixing plate is arranged at one end, far away from the third telescopic rod, of the supporting rod, a negative pressure sucker is arranged on one side, close to the fixing mechanism, of the fixing plate, and is connected with a negative pressure generator which is electrically connected with the control system.
After the technical scheme is adopted, negative pressure is generated in the negative pressure sucker through the negative pressure generator, the cover plate is sucked through the sucker negative pressure sucker, then the third telescopic column is started to stretch out and draw back, the cover plate is separated from the detection plate, then the third support rod is driven to rotate through the rotating seat, the cover plate is rotated away from the upper side of the detection plate, and the cover plate is prevented from affecting the liquid adding device to add liquid.
Preferably, the moving mechanism is a track-type moving device, the track-type moving device comprises a track for entering the cleaning chamber from the sample feeding port and then entering other chambers from the cleaning chamber, a sliding part in the track is fixedly connected with the fixing device, the track-type moving device comprises a driving part for driving the sliding part to move in the track according to instructions, and the driving part is electrically connected with the control system.
After the technical scheme is adopted, the track type moving device is similar to a track type medicine delivery device arranged on a ceiling and used by traditional Chinese medicine institutions in the prior art.
Preferably, a closing door which can be opened and closed is arranged between the chambers, and an opening and closing mechanism for driving the closing door to open and close is electrically connected with the control system.
After the technical scheme is adopted, the independence among the chambers is ensured through the sealing door, so that the incubation temperature of each chamber is convenient to set.
In summary, due to the adoption of the technical scheme, the beneficial effects of the invention are as follows:
1. the detection plate with the sample is placed into a fixing mechanism of a control device from a sample inlet, the fixing mechanism is sequentially moved to each chamber by the moving mechanism according to a set program, for example, after the fixing mechanism is moved to a cleaning chamber and then to a position corresponding to a liquid adding device in the cleaning chamber according to the set program, a control system controls a cover plate opening mechanism to open a cover plate of the detection plate, then cleaning liquid is dripped into the detection plate through the liquid adding device, after the cleaning is finished, the waste liquid is sucked into a waste liquid barrel through the liquid adding device, after the cleaning is repeatedly performed for several times according to the set program, the fixing device after the cleaning is finished is moved to a designated position of a closed liquid incubation chamber by the moving mechanism, sealing liquid is dripped into the liquid incubation chamber through the liquid adding device of the closed liquid incubation chamber, then the cover plate is covered on the detection plate by the cover plate opening device for incubation, volatilization of the closed liquid is avoided, after the incubation is finished, the fixing device is moved to the cleaning chamber again, all steps are completed according to the set program, the sealing plate is removed from the sampling port after the sealing is finished, the sealing plate is scanned, and the detection plate is effectively saved.
2. The common glass slide only comprises a marking frosting area and a tissue slice preparation area, a tissue slice sample is usually prepared or a plurality of tissue slice samples are prepared in the same area under special conditions, when the tissue slice sample is dyed (immunohistochemical or special dyeing), the tissue slice sample can be partitioned by means of different methods (a combining pen and the like), but only one type of dyeing of the same sample can be realized, and when an antibody and a dyeing liquid are used, the waste is serious, and the dyeing cost is increased. The slide glass is provided with a plurality of sample Kong Ranse areas at the bottom of the slice and is matched with the sample holes on the detection plate, the same sample can be divided into a plurality of areas, and detailed labeling can be carried out according to the sample hole marks, so that the phenomenon of misaddition during the addition of antibodies or staining solution in the staining process is prevented. If the glass slide is used for preparing tissue sections for dyeing and is combined with a detection plate, the simultaneous performance of multiple antibodies or multiple dyeings of the same glass slide and the same sample or multiple dyeings of multiple samples can be realized, and the time required for dyeing is greatly saved. Meanwhile, the use of antibodies or staining solution can be saved to the greatest extent due to small area of the detection sample holes, and the staining cost can also be saved.
Drawings
FIG. 1 is a schematic view of the external structure of the present invention;
FIG. 2 is a schematic diagram of a moving mechanism according to the present invention;
FIG. 3 is a schematic structural view of the fixing device of the present invention;
FIG. 4 is a schematic structural view of a detection plate matched with the all-in-one machine of the present invention;
FIG. 5 is a schematic view of a slide mated with the all-in-one machine of the present invention;
FIG. 6 is a schematic view of the liquid feeding apparatus of the present invention;
FIG. 7 is a schematic diagram of the side of FIG. 6;
FIG. 8 is a schematic structural view of the fixing base of FIG. 6;
reference numerals
The device comprises a 1-cleaning chamber, a 2-closed liquid incubation chamber, a 3-primary-antibody incubation chamber, a 4-sample inlet, a 5-secondary-antibody incubation chamber, a 6-sample outlet, a 7-sealing chamber, an 8-transparent chamber, a 9-color development chamber, a 10-waste liquid barrel, an 11-control button, a 12-base, a 13-track, a 14-first telescopic rod, a 15-pressing plate, a 16-drain hole, a 17-air inlet, a 18-third telescopic rod, a 19-supporting rod, a 20-fixing plate, a 21-first groove, a 22-fixing bottom plate, a 23-protruding part, a 24-second groove, a 25-clamping groove, a 26-porous plate, a 27-second sample hole, a 28-cover plate, a 29-first sample hole, a 30-glass slide, a 31-fixing seat, a 32-liquid storage groove, a 33-water inlet hole, a 34-second telescopic rod, a 35-mounting seat, a 36-bar, a 37-liquid adding channel, a 38-liquid adding needle, a 39-liquid adding needle and a 40-liquid adding needle.
Detailed Description
For the purposes of making the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, which are generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, as provided in the accompanying drawings, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, are intended to be within the scope of the present application.
In the description of the embodiments of the present application, it should be noted that, directions or positional relationships indicated by terms such as "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use of the inventive product, are merely for convenience of description and simplicity of description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be configured and operated in a specific direction, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
The integrated machine comprises a sample inlet 4 communicated with the washing chamber 1 and a sample outlet 6 communicated with the sealing chamber 7, liquid adding devices are arranged in the washing chamber 1, the sealing liquid incubating chamber 2, the first anti-incubating chamber 3, the second anti-incubating chamber 5, a color developing chamber 9, the transparent chamber 8 and the sealing chamber 7, the liquid adding devices are connected with a waste liquid barrel 10 arranged on the outer surface of the integrated machine, a fixing mechanism used for fixing a detection plate is arranged in the integrated machine, the fixing mechanism comprises a fixing mechanism and a cover plate opening mechanism used for opening a cover plate 28 of the detection plate, a control system is arranged in the integrated machine, the control system is electrically connected with the moving mechanism, the fixing device and the control system, the control system is arranged on the outer surface of the integrated machine, and the control system is electrically connected with a button 11 arranged on the outer surface of the integrated machine.
In this embodiment, the reagent added in the liquid storage tank 32 of the liquid adding mechanism of the closed liquid incubation chamber 2 is a closed liquid, the reagent added in the liquid storage tank 32 of the liquid adding mechanism of the cleaning chamber 1 is a PBS solution, the reagent added in the liquid storage tank 32 of the liquid adding mechanism of the first anti-incubation chamber 3 is an antibody I (different antibodies I can be added into different liquid storage tanks 32 according to the need), the reagent added in the liquid storage tank 32 of the liquid adding mechanism of the second anti-incubation chamber 5 is an antibody II, the reagent added in the liquid storage tank 32 of the liquid adding mechanism of the color developing chamber 9 is a color developing liquid, the reagent added in the liquid storage tank 32 of the liquid adding mechanism of the transparent chamber 8 is xylene, and the reagent added in the liquid storage tank 32 of the liquid adding mechanism of the film sealing chamber 7 is a film sealing liquid.
In this embodiment, the all-in-one machine includes supporting pick-up plate and slide glass 30, the pick-up plate includes fixed baseplate 22, perforated plate 26 and apron 28, offer a plurality of second recesses 24 that are used for placing slide glass 30 on the fixed baseplate 22, fixed baseplate 22 and apron 28 are dismantled and are connected, fixed baseplate 22 and perforated plate 26 joint, the front of slide glass 30 includes mark dull polish region and tissue section preparation district, a plurality of first sample holes 29 have been offered on the tissue section preparation district, be provided with a plurality of second sample holes 27 with first sample hole 29 complex on the perforated plate 26, first sample hole 29 and second sample hole 27 all are the matrix arrangement, and the quantity of second sample hole 27 is 96 in this embodiment, and 96 second sample holes 27 form the matrix of 12 rows 8.
In this embodiment, the number of the second grooves 24 is four, and the bottom of the second groove 24 is laid with an elastic flexible waterproof soft film, the fixing base plate 22 is provided with a clamping groove 25, the bottom of the porous plate 26 is provided with a protrusion matched with the clamping groove 25, and the lower surface of the porous plate 26 is laid with a flexible waterproof soft film substrate.
In this embodiment, the fixing mechanism includes a base 12, a first groove 21 for placing a detection plate is provided on the base 12, at least two first telescopic rods 14 are symmetrically provided on the base 12, pressing plates 15 are fixedly provided at the upper ends of the first telescopic rods 14, protruding portions 23 are symmetrically provided at two sides of the detection plate, and a pushing mechanism for pushing the detection plate out of the first groove 21 when the fixing device moves the seal piece chamber 7 is provided at the bottom of the detection plate.
In this embodiment, the depth of the first recess 21 is greater than the height of the detection plate, the push-out mechanism includes two spring telescopic columns symmetrically disposed in the first recess, one end of the spring telescopic column is fixedly connected with the bottom of the first recess, and a vibrator is fixedly disposed at the bottom of the first recess 21 for driving the detection plate to vibrate.
In this embodiment, be provided with several wash port 16, several air intake 17 and several air exit in the first recess 21, wash port 16 links to each other with waste liquid bucket 10 through the pipeline, be provided with the second drainage pump in the pipeline between wash port 16 and the waste liquid bucket 10, several air intake 17 and several air exit set up relatively, air intake 17 passes through the pipeline and links to each other with the gas purification plant who sets up in the all-in-one, be provided with the air inlet pump in the pipeline, the air inlet pump is connected with control system electricity, the air exit communicates with external pipeline.
In this embodiment, the liquid feeding device includes fixing base 31 fixedly disposed in each room, be provided with several mount pad 35 on the fixing base 31, every the mount pad 35 all links to each other through at least one second telescopic link 34 with the fixing base 31, every all be provided with several liquid feeding stick 36 on the mount pad 35, the quantity of liquid feeding stick 36 on every mount pad 35 and the quantity of mount pad 35 correspond with the number of rows and the column number of second sample hole 27 on perforated plate 26 respectively, the one end of liquid feeding stick 36 is provided with liquid feeding needle 39 and flowing back needle 40, be provided with in the liquid feeding stick 36 with liquid feeding passageway 37 and flowing back passageway 38 that liquid feeding needle 39 and flowing back needle 40 communicate respectively, liquid feeding passageway 37 has the liquid storage tank 32 that sets up in the fixing base 31 through the pipe connection, the quantity of liquid storage tank 32 corresponds with the quantity of liquid feeding stick 36, all be provided with flowmeter and first electric valve in the liquid feeding passageway 37, the flowing back passageway 38 links to each other with the first liquid pump that sets up in the mount pad 35 through the pipe, first liquid pump links to each other with electric valve 10, first electric control system and the first liquid pump through the pipe.
In this embodiment, the top of fixing base 31 is provided with the third recess, the top of all-in-one is provided with the upper cover that communicates with the third recess, reservoir 32 is the matrix form and evenly sets up in the third recess, the lateral wall of third recess is provided with several water inlet 33, water inlet 33 all links to each other with outside water supply system through the pipeline, be provided with the second electrically operated valve on the pipeline, the second electrically operated valve is connected with the control system electricity.
In this embodiment, the apron opening mechanism includes the fixed roating seat that sets up on fixed establishment, fixedly connected with third telescopic link 18 on the roating seat, the flexible direction perpendicular to fixed establishment place plane of third telescopic link 18, the fixed branch 19 that is provided with in top of third telescopic link 18, the plane that branch 19 is located and the plane level that fixed establishment is located, and the plane that branch 19 is located the top of fixed establishment place plane, the one end that branch 19 kept away from third telescopic link 18 is provided with fixed plate 20, one side that fixed plate 20 is close to fixed establishment is provided with negative pressure sucking disc, negative pressure sucking disc is connected with negative pressure generator, negative pressure generator and control system electricity are connected.
In this embodiment, the moving mechanism is a track-type moving device, the track-type moving device includes a track 13 for entering the cleaning chamber 1 from the sample feeding port and then entering other chambers from the cleaning chamber 1, the sliding component in the track 13 is fixedly connected with the fixing device, the track-type moving device includes a driving component for driving the sliding component to move in the track 13 according to the instruction, and the driving component is electrically connected with the control system.
In this embodiment, temperature controllers are disposed in the closed liquid incubation chamber 2, the primary anti-incubation chamber 3 and the secondary anti-incubation chamber 5, and the temperature controllers are electrically connected with a control system.
In this embodiment, a openable and closable closing door is disposed between each chamber, and an opening and closing mechanism for driving the closing door to open and close is electrically connected with the control system.
Referring to fig. 1 to 8, the method of use of the present invention is as follows:
step 1: opening the door cover of the sample inlet 4, starting the first telescopic rod 14 to extend upwards after the control system detects that the door cover of the sample inlet 4 is opened, so that the prepared detection plate is conveniently placed in the first placing groove 21, the detection plate is placed on the spring telescopic column, and then closing the door cover of the sample inlet 4;
step 2: after the control system detects that the door cover of the sample inlet 4 is closed, the first telescopic rod 14 is controlled to be contracted downwards, so that the pressure plate presses the detection plate downwards, and the spring telescopic column is contracted until the detection plate completely enters the first groove 21;
step 3: the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the cleaning chamber 1 according to the set program, then stops moving, then controls the third telescopic rod 18 to move downwards, enables the negative pressure sucker on the fixed plate 20 to be in contact with the cover plate 28, then starts the negative pressure generator, enables the cover plate 28 to be absorbed by the negative pressure sucker, controls the third telescopic rod 18 to move upwards after being absorbed, enables the cover plate 28 to be separated from the fixed bottom plate 22, and then starts the rotating seat, and the rotating seat drives the third telescopic rod 18 to rotate, so that the cover plate 21 is separated from the upper part of the fixed bottom plate 22;
step 4: after the cover plate 21 is opened, the control system controls the second telescopic rod 34 to extend, so that the liquid adding rod 36 on the mounting seat 35 moves downwards, the liquid adding needle and the liquid discharging needle extend to the bottom of the second sample hole 27 along the wall of the second sample hole 27, the washing liquid is injected into the selected second sample hole 27, the washing liquid enters the first sample hole 29 filled with the sample, then the second telescopic rod 34 is controlled to shrink, the liquid adding rod 36 is reset, after the detection plate is completely filled with the liquid, the control system controls the vibrator to drive the detection plate to vibrate, the second telescopic rod 34 is controlled to extend again when the detection plate vibrates for 10-20 seconds, the time for washing is finished, the control system controls the liquid adding needle and the liquid discharging needle to extend to the bottom of the second sample hole 27 along the wall of the second sample hole 27, simultaneously, the first liquid discharging pump is started, the waste is sucked into the waste liquid barrel 10 from the liquid discharging needle 40, and then the steps are repeated, and the cleaning is finished repeatedly.
Step 5: after the cleaning is finished, the control system drives the base 12 to move to the position right below the liquid adding device of the closed liquid incubation chamber 2 according to the set program control driving piece, then the movement is stopped, the liquid adding step is repeated, after the liquid adding is finished, the control system controls the starting rotary seat, the rotary seat drives the third telescopic rod 18 to rotate, so that the cover plate 21 reaches the upper part of the fixed bottom plate 22, then the third telescopic rod 18 is downwards moved, the cover plate 21 is covered on the detection plate for incubation, the temperature controller controls the temperature in the closed liquid incubation chamber 2 to be 37 ℃ according to the set incubation temperature, and the incubation is performed for 1 hour until the incubation is finished;
step 6: after the incubation of the sealing liquid is finished, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the cleaning chamber 1 according to the set program, then the movement is stopped, then the step 3 and the step 4 are repeated, and the cleaning step is finished until the cleaning is finished.
Step 7: after the cleaning is finished, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the first anti-incubation chamber 3 according to the set program, the operation of the step 5 is repeated, the first anti-incubation temperature is 37 ℃, the incubation time is 1-2 hours, after the incubation is finished, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the cleaning chamber 1 according to the set program, then the movement is stopped, then the step 3 and the step 4 are repeated, and the cleaning step is completed until the cleaning is finished;
step 8: after the cleaning is finished, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the secondary antibody incubation chamber 5 according to the set program, the operation of the step 5 is repeated, the secondary antibody incubation temperature is 37 ℃, the incubation time is 1-2 hours, after the incubation is finished, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the cleaning chamber 1 according to the set program, then the movement is stopped, then the step 3 and the step 4 are repeated, and the cleaning step is completed until the cleaning is finished;
step 9: after the cleaning is finished, the control system drives the base 12 to move to the position right below the liquid adding device of the color developing chamber 9 according to the set program control driving piece, the operation of the step 5 is repeated, then color development is carried out for 1-3 minutes, after the color development is finished, the control system drives the base 12 to move to the position right below the liquid adding device of the cleaning chamber 1 according to the set program control driving piece, then the movement is stopped, then the step 3 and the step 4 are repeated, and the cleaning step is completed until the cleaning is finished;
step 10: after the cleaning is finished, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the transparent chamber 8 according to the set program, the operation of the step 5 is repeated, and then the transparent is carried out for 2 times, and each time is carried out for 1-2min;
step 11: after the transparency is finished, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the sealing piece chamber 7 according to the set program, and the operation of the step 5 is repeated to finish sealing pieces;
step 12: after the sealing sheet is finished, the control system sends out a reminding sound, an operator opens the door cover of the sampling port 6, after detecting that the door cover of the sampling port 6 is opened, the control system starts the first telescopic rod 14 to extend upwards, and the spring telescopic column jacks up the detection plate, so that the detection plate is taken out conveniently.
This immune group ization all-in-one has self-cleaning function, and operating personnel accessible control button starts self-cleaning function, and self-cleaning function's principle is as follows:
after the self-cleaning function is started, the control system firstly controls the fixing device to reach the chambers in which reagents need to be replaced, if all the chambers need to be cleaned, the chambers are sequentially reached, the first incubation chamber 3 is taken as an example, the control system controls the driving piece to drive the base 12 to move to the position right below the liquid adding device of the cleaning chamber 1 according to the set program, then the movement is stopped, the second telescopic rod 34 is controlled to stretch and retract, the liquid adding needle 39 and the liquid discharging needle 40 on the liquid adding rod 36 reach the first groove 21, then the second electric valve is opened, water of an external water supply system enters the third groove from the water inlet 33, at the moment, the first electric valve is in an opened state, water enters the liquid storage grooves 32 from the third groove, the liquid storage grooves 32 and the liquid adding channels 37 are flushed, the flushed waste water is discharged from the liquid adding needle to the first groove 21 through the water discharging holes 16 in the first groove 21, then the first drainage pump and the second drainage pump are started, the waste water in the first groove 21 is discharged to the waste liquid barrel until the cleaning of the chambers is finished, the water of the other chambers is moved to the third grooves is started, at the moment, the first air pump is blown down to the grooves 21 after the cleaning is finished, and the cleaning of the chambers is finished.
The foregoing examples merely represent specific embodiments of the present application, which are described in more detail and are not to be construed as limiting the scope of the present application. It should be noted that, for those skilled in the art, several variations and modifications can be made without departing from the technical solution of the present application, which fall within the protection scope of the present application.
Claims (6)
1. An immune group ization all-in-one, its characterized in that: the integrated machine comprises a sample inlet (4) communicated with the cleaning chamber (1) and a sample outlet (6) communicated with the sealing chamber (7), wherein a liquid adding device is arranged in the cleaning chamber (1), the sealing liquid incubation chamber (2), the primary anti-incubation chamber (3), the secondary anti-incubation chamber (5), the liquid adding device (9), the transparent chamber (8) and the sealing chamber (7), a waste liquid barrel (10) arranged on the outer surface of the integrated machine is connected with the liquid adding device, a fixing device used for fixing a detection plate is arranged in the integrated machine, the fixing device is connected with a moving mechanism used for driving the fixing device to sequentially reach the designated positions of the chambers, the fixing device comprises a fixing mechanism and a cover plate opening mechanism used for opening the detection plate (28), a control system is arranged in the integrated machine, and the control system, the fixing device and the control system are connected with each other through the control system and the control system;
the integrated machine comprises a matched detection plate and a glass slide (30), wherein the detection plate comprises a fixed bottom plate, a porous plate (26) and a cover plate (28), a plurality of second grooves (24) for placing the glass slide (30) are formed in the fixed bottom plate, the fixed bottom plate is detachably connected with the cover plate (28), the fixed bottom plate is clamped with the porous plate (26), the front surface of the glass slide (30) comprises a marking frosted area and a tissue slice preparation area, a plurality of first sample holes (29) are formed in the tissue slice preparation area, a plurality of second sample holes (27) matched with the first sample holes (29) are formed in the porous plate (26), and the first sample holes (29) and the second sample holes (27) are all arranged in a matrix;
the fixing mechanism comprises a base (12), a first groove (21) for placing a detection plate is formed in the base (12), at least two first telescopic rods (14) are symmetrically arranged on the base (12), pressing plates (15) are fixedly arranged at the upper ends of the first telescopic rods (14), protruding parts (23) are symmetrically arranged on two sides of the detection plate, and a pushing mechanism for pushing the detection plate out of the first groove (21) when the fixing device moves the seal piece chamber (7) is arranged at the bottom of the detection plate;
the device is characterized in that a plurality of drain holes (16), a plurality of air inlets (17) and a plurality of air outlets are arranged in the first groove (21), the drain holes (16) are connected with the waste liquid barrel (10) through pipelines, a second liquid discharge pump is arranged in the pipeline between the drain holes (16) and the waste liquid barrel (10), the plurality of air inlets (17) and the plurality of air outlets are oppositely arranged, the air inlets (17) are connected with a gas purifying device arranged in the integrated machine through the pipeline, an air inlet pump is arranged in the pipeline, the air inlet pump is electrically connected with a control system, and the air outlets are communicated with an external pipeline;
the liquid feeding device comprises fixing seats (31) fixedly arranged in each chamber, a plurality of mounting seats (35) are arranged on the fixing seats (31), each mounting seat (35) is connected with the fixing seat (31) through at least one second telescopic rod (34), each mounting seat (35) is provided with a plurality of liquid feeding rods (36), the number of the liquid feeding rods (36) on each mounting seat (35) and the number of the mounting seats (35) are respectively corresponding to the number of the rows and the number of the columns of the second sample holes (27) on the porous plate (26), one end of each liquid feeding rod (36) is provided with a liquid feeding needle (39) and a liquid discharging needle (40), a liquid feeding channel (37) and a liquid discharging channel (38) which are respectively communicated with the liquid feeding needle (39) and the liquid discharging needle (40) are arranged in each liquid feeding rod (36), each liquid feeding channel (37) is connected with a liquid storage groove (32) arranged in the fixing seats (31) through a pipeline, the number of the liquid feeding rods (36) corresponds to the number of the liquid feeding rods (37), one end of each liquid feeding rod (36) is respectively corresponding to the number of the liquid feeding rods (37) on the second sample holes (27), one end of each liquid feeding rod is provided with an electric valve, and the electric valve (37) is connected with the liquid discharging channel (35) through one electric pipeline, and the liquid discharging channel is connected with the liquid pump (10 through one electric flowmeter (10) respectively, and the liquid pump is arranged in the liquid discharging channel The second telescopic rod (34) and the first liquid discharge pump are electrically connected with a control system.
2. An immunohistochemical all-in-one machine according to claim 1, wherein: the top of fixing base (31) is provided with the third recess, the top of all-in-one is provided with the upper cover with third recess intercommunication, reservoir (32) are matrix form evenly set up in the third recess, the lateral wall of third recess is provided with several water inlet (33), water inlet (33) all link to each other with outside water supply system through the pipeline, be provided with the second electric valve on the pipeline, the second electric valve is connected with the control system electricity.
3. An immunohistochemical all-in-one machine according to claim 1, wherein: the cover plate opening mechanism comprises a rotating seat fixedly arranged on the fixing mechanism, a third telescopic rod (18) is fixedly connected to the rotating seat, the telescopic direction of the third telescopic rod (18) is perpendicular to the plane where the fixing mechanism is located, a supporting rod (19) is fixedly arranged at the top of the third telescopic rod (18), the plane where the supporting rod (19) is located is horizontal to the plane where the fixing mechanism is located, the plane where the supporting rod (19) is located above the plane where the fixing mechanism is located, a fixing plate (20) is arranged at one end, far away from the third telescopic rod (18), of the supporting rod (19), a negative pressure sucker is arranged on one side, close to the fixing mechanism, of the fixing plate (20), and is connected with a negative pressure generator which is electrically connected with a control system.
4. An immunohistochemical all-in-one machine according to claim 1, wherein: the moving mechanism is a track type moving device, the track type moving device comprises a track (13) used for entering the cleaning chamber (1) from the sample feeding port and then entering other chambers from the cleaning chamber (1), a sliding part in the track (13) is fixedly connected with the fixing device, the track type moving device comprises a driving part used for driving the sliding part to move in the track (13) according to instructions, and the driving part is electrically connected with the control system.
5. The immunohistochemical all-in-one machine of claim 4, wherein: temperature controllers are arranged in the closed liquid incubation chamber (2), the primary anti-incubation chamber (3) and the secondary anti-incubation chamber (5), and are electrically connected with a control system.
6. An immunohistochemical all-in-one machine according to claim 1, wherein: and an openable closing door is arranged between the chambers, and an opening and closing mechanism for driving the closing door to open and close is electrically connected with the control system.
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| CN202211610285.1A CN116008574B (en) | 2022-12-14 | 2022-12-14 | Immune group ization all-in-one |
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