CN116004881A - Molecular marker highly closely linked with corn ear position and application thereof - Google Patents
Molecular marker highly closely linked with corn ear position and application thereof Download PDFInfo
- Publication number
- CN116004881A CN116004881A CN202210888929.7A CN202210888929A CN116004881A CN 116004881 A CN116004881 A CN 116004881A CN 202210888929 A CN202210888929 A CN 202210888929A CN 116004881 A CN116004881 A CN 116004881A
- Authority
- CN
- China
- Prior art keywords
- corn
- seq
- molecular marker
- ear position
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a molecular marker closely linked with the high position of corn ears and application thereof. The molecular marker highly closely linked with the corn ear position is a DNA fragment shown as SEQ ID No.3 or a DNA fragment shown as SEQ ID No.4, and can be detected by using a primer pair consisting of two single-stranded DNAs shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table. Experiments prove that the molecular marker closely linked with the corn ear position is related to the corn ear position, the corn ear position condition can be successfully identified by using the molecular marker, and the molecular marker has the advantages of simplicity, convenience, rapidness, high efficiency and accuracy, and is good in repeatability and high in specificity, can be used for corn molecular marker assisted breeding, and can be used for breeding new corn varieties with excellent comprehensive properties, so that the breeding cost is greatly saved, and the breeding efficiency is improved.
Description
Technical Field
The invention relates to a molecular marker closely linked with the corn ear position and application thereof in the field of biotechnology.
Background
Corn (Zea mays l.) is a dual-purpose crop for food, feed and economy, and is the only crop in our country that steadily increases in sowing area and yield. In recent years, global economy has rapidly developed, and demand for corn is expected to increase in future industrial production and life. The planting area of the corn in 2021 is 4332 ten thousand hectares, which is increased by 206 ten thousand hectares compared with the last year, the yield is up to 27255 ten thousand tons, and the yield is about 39.91 percent of the total yield of the grain in China. Therefore, corn production has very important strategic position in guaranteeing the grain safety of China.
The ear height is one of the important agronomic traits that constitute the ideal plant type of corn. Research shows that the high corn ear position can seriously reduce the corn planting density, lodging resistance and harvest index; too low, the ventilation and ventilation in the field are not facilitated, the plant disease and insect pest infection rate is increased, and the biological yield is reduced. Therefore, the genetic basis of high corn ear position is further analyzed, and the molecular marker closely linked with the ear position high quantitative trait locus (quantitative trait loci, QTL) is developed, so that the method has important significance for breeding new corn varieties with ideal plant types and high yield.
The molecular markers have the advantages of large quantity, no influence of environmental conditions, development period, expression regulation and control and other factors, capability of providing complete and rich genetic information and the like, and are widely applied to aspects of germplasm resource identification, QTL positioning, molecular marker auxiliary selection and the like. InDel (InDel) markers are one of the commonly used molecular markers based on differences in DNA levels, specifically differences in two materials, a certain number of nucleotide insertions or deletions in certain loci of the genome of one material relative to the other, and PCR primers are designed to amplify these InDel sites based on their InDel sites. By utilizing InDel markers closely linked with target genes, assisted backcross selection, assisted pedigree selection and even whole genome selection are adopted, so that linkage encumbrance is reduced, favorable genes are polymerized, the breeding process is accelerated, and the selection efficiency and effect can be effectively improved.
At present, although QTLs for controlling the height of corn ears are reported on all chromosomes of corn, few reports for developing molecular markers closely linked with target QTLs and applying for patent applications exist, and no patent report related to the height of corn ears exists in a qEH1-2 segment.
Disclosure of Invention
The invention aims to solve the technical problem of how to detect the high-quality character of corn ears.
In order to solve the technical problems, the invention firstly provides application of a substance for detecting the corn cob position high molecular marker in detecting or assisting in detecting the corn cob position high character, wherein the corn cob position high molecular marker is a DNA fragment shown as SEQ ID No.3 and a DNA fragment shown as SEQ ID No.4.
In the application, the substance for detecting the corn cob position high molecular marker can be a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in the sequence table.
The invention also provides a method for detecting the corn ear position high character, which comprises the following steps: the genome DNA of corn to be detected is used as a template, a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table is utilized for PCR amplification, the ear position of the obtained homozygous corn with the PCR product sequence of SEQ ID No.3 is lower than or candidate lower than that of the obtained homozygous corn with the PCR product sequence of SEQ ID No.4, the ear position of the obtained homozygous corn with the PCR product sequence of SEQ ID No.3 is lower than or candidate lower than that of the obtained heterozygous corn with the PCR product sequences of SEQ ID No.3 and SEQ ID No.4, and the ear position of the obtained heterozygous corn with the PCR product sequences of SEQ ID No.3 and SEQ ID No.4 is lower than or candidate lower than that of the obtained homozygous corn with the PCR product sequence of SEQ ID No.4.
The invention also provides a method for detecting the corn ear position high character, which comprises the following steps: the genome DNA of corn to be detected is used as a template, and PCR amplification is carried out by using a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table, the ear height of the obtained homozygous corn with the size of 329bp is lower than or lower than that of the obtained homozygous corn with the size of 277bp, the ear height of the obtained homozygous corn with the size of 329bp is lower than or lower than that of the obtained heterozygous corn with the size of 329bp and 277bp, and the ear height of the obtained heterozygous corn with the size of 329bp and 277bp is lower than or lower than that of the obtained homozygous corn with the size of 277 bp.
The invention also provides a corn breeding method, which comprises the following steps: and (3) taking genome DNA of the corn to be detected as a template, carrying out PCR amplification by using a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table, and selecting the corn to be detected with a PCR product of SEQ ID No.3 as a parent to finish breeding.
Above, the use of the sequences from the sequence ListingThe reaction system for PCR amplification of the primer pair consisting of two single-stranded DNA shown in SEQ ID No.1 and SEQ ID No.2 can be as follows: 1. Mu.L of single-stranded DNA shown in SEQ ID No.1 at a concentration of 10. Mu. Mo 1/L; 1. Mu.L of single-stranded DNA shown in SEQ ID No.2 at a concentration of 10. Mu. Mo 1/L; 1. Mu.L of genomic DNA at a concentration of 100 ng/. Mu.L; 5 μL 2X Taq PCR StarMix with Loading Dye (Beijing Kang Runcheng Biotechnology Co., ltd., product number: A012-01); 2 mu L ddH 2 O。
The reaction conditions for PCR amplification using a primer pair consisting of two single-stranded DNAs shown as SEQ ID No.1 and SEQ ID No.2 in the sequence Listing may be: pre-denaturation at 95℃for 10min; denaturation at 95℃for 45s, annealing at 58℃for 45s, elongation at 72℃for 60s,35 cycles; extending at 72 ℃ for 10min; preserving at 4 ℃.
The application of the substance for detecting the corn cob position high molecular marker in preparing the product for detecting the corn cob position high character also belongs to the protection scope of the invention.
The application of the corn cob position high molecular marker in detecting or assisting in detecting corn cob position high characters also belongs to the protection scope of the invention.
The application of the corn ear position high molecular marker in corn breeding also belongs to the protection scope of the invention.
In the invention, the spike height is the height from the ground to the female spike node.
The corn ear position high molecular marker is related to the corn ear position, can be used for successfully identifying the corn ear position high condition, has the advantages of simplicity, convenience, rapidness, high efficiency and accuracy, has good repeatability and high specificity, can be used for corn molecular marker assisted breeding, and can be used for breeding new corn varieties with excellent comprehensive properties, thereby greatly saving the breeding cost and improving the breeding efficiency.
The following detailed description of the invention is provided in connection with the accompanying drawings that are presented to illustrate the invention and not to limit the scope thereof. The examples provided below are intended as guidelines for further modifications by one of ordinary skill in the art and are not to be construed as limiting the invention in any way.
Drawings
FIG. 1 shows the result of alignment of amplified sequences of the molecular markers qEH1-2 of the present invention in parents.
FIG. 2 is an electropherogram of PCR amplified products of molecular markers qEH1-2 of the present invention in parents. Wherein W is the amplified band type of the maize inbred line W22, C is the amplified band type of the maize wild inbred species CIMMYT8759, and the band sizes of markers are 100bp, 250bp, 500bp, 750bp, 1000bp, 1500bp and 2000bp from bottom to top in sequence.
FIG. 3 shows the molecular marker qEH1-2 of the present invention at F 2 Electropherograms of PCR amplified products in the population. Wherein, W is the amplified band type of homozygous W22 genotype, C is the amplified band type of homozygous CIMMYT8759 genotype, H is the amplified band type of heterozygous genotype, and the Marker sizes are 100bp, 250bp, 500bp, 750bp, 1000bp, 1500bp and 2000bp from bottom to top.
FIG. 4 shows the molecular marker qEH1-2 of the present invention at F 2 Single marker analysis result of spike position high character in colony. Nil_w22 represents homozygous W22 genotype, het represents heterozygous genotype, nil_cimmmyt 8759 represents homozygous cimmmyt 8759 genotype, x represents a significant difference (P<0.05 Representing that the difference is extremely significant (P<0.01)。
Detailed Description
The experimental methods in the following examples, unless otherwise specified, are conventional methods, and are carried out according to techniques or conditions described in the literature in the field or according to the product specifications. Materials, reagents, instruments and the like used in the examples described below are commercially available unless otherwise specified. The quantitative tests in the following examples were all set up in triplicate and the results averaged. In the following examples, unless otherwise specified, the 1 st position of each nucleotide sequence in the sequence listing is the 5 'terminal nucleotide of the corresponding DNA/RNA, and the last position is the 3' terminal nucleotide of the corresponding DNA/RNA.
The maize inbred line W22 and the maize wild inbred species CIMMYT8759 in the examples described below are both described in the literature (Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem, JItergreplant biol.2016Jan;58 (1): 81-90.Doi:10.1111/jipb.12358.Epub 2015Jul 16.) and CIMMYT8759 is CIMMYT access 8759 in this literature, and CIMMYT8759 is available to the public from the applicant for use only in experiments related to the duplicate invention and not as a further use.
MR0919 in the following examples is a U.S. corn germplasm resources center (Maize Genetics Cooperation Stock Center) product with the website: www.maizecoop.cropsci.uiuc.edu the public can also obtain this biological material from the applicant, which is used only for repeated experiments related to the invention and not as other uses. MR0919 is an introgressed material derived from maize inbred line W22 by crossing, backcrossing and selfing with maize wild inbred CIMMYT 8759.
Example 1: molecular marker closely linked with corn ear position
The invention provides a molecular marker qEH-2 (marked as corn cob position high molecular marker) for identifying or assisting in identifying corn cob position high molecular marker, wherein the molecular marker is a DNA fragment obtained by taking corn genome DNA as a template and carrying out PCR amplification on A1 by using a primer pair, and the sequence of the obtained DNA fragment is SEQ ID No.3 or SEQ ID No.4. The primer set A1 has the following sequence:
forward amplification primers EH1-F:5'-ACAGTGCCAGCTTTGATGTT-3', as shown in SEQ ID No. 1;
reverse amplification primer EH1-R:5'-ACAACTGTCCAAGAGAGCCA-3', as shown in SEQ ID No. 2;
the sequence of the obtained PCR product is detected by taking the genomic DNA of a corn inbred line W22 with lower spike height and a corn wild kindred species CIMMYT8759 with higher spike height as templates and carrying out PCR amplification by using a forward amplification primer shown as SEQ ID No.1 and a reverse amplification primer shown as SEQ ID No. 2.
Wherein, the reaction system of 10 mu L PCR amplification is as follows:
(1) 1. Mu.L of forward amplification primer shown in SEQ ID No.1 at a concentration of 10. Mu.m 1/L;
(2) 1. Mu.L of the reverse amplification primer shown in SEQ ID No.2 at a concentration of 10. Mu.m 1/L;
(3) 1. Mu.L of DNA template at a concentration of 100 ng/. Mu.L;
(4) 5 μL 2X Taq PCR StarMix with Loading Dye (Beijing Kang Runcheng Biotechnology Co., ltd., product number: A012-01);
(5)2μL ddH 2 O。
the procedure for PCR amplification was as follows:
(1) Pre-denaturation at 95℃for 10min;
(2) Denaturation at 95℃for 45s, annealing at 58℃for 45s, elongation at 72℃for 60s,35 cycles;
(3) Extending at 72 ℃ for 10min;
(4) Preserving at 4 ℃.
PCR instrument model: eppendorf Mastercycler nexus.
The PCR amplified product is subjected to electrophoresis separation in 4.0% agarose gel (each 100mL of gel solution contains 4.0g agarose), sequencing analysis is carried out, and the result shows that the PCR amplified product is subjected to PCR amplification by taking genomic DNA of a maize inbred line W22 as a template, the molecular weight of the PCR amplified product is 329bp, and the nucleotide sequence of the PCR amplified product is shown as SEQ ID No. 3; the genome DNA of wild kindred species CIMMYT8759 of corn is used as a template for PCR amplification, the molecular weight of a PCR amplification product is 277bp, and the nucleotide sequence of the PCR amplification product is shown as SEQ ID No.4.
SEQ ID No.3：
ACAGTGCCAGCTTTGATGTTCTGTTAGAACTTTGAAGTTTCATTTGATACTTGTCCTTTGGATAAGTAGTCAAACATTGTAGTGAATATGGGTAGCAATAGCACAACTGGAGGTGGAAAGTATGTTGGTTAAGTAAGAATTAGGTAGCAATAGCACAACTGGAAGTGGGAAGTATGTTGGTTAAGTAAGAATTAGGTAGCAATAGCACAACTGGAAGTGGGAAGTATGTTGGTTAAGTAAGAATTAGGTAGCAATAGCAACAGGCAACAGCTACATTTCATGCCATGGTTACAGCTTTGCATGTTCAATTGGCTCTCTTGGACAGTTGT。
SEQ ID No.4：
ACAGTGCCAGCTTTGATGTTCTGTTAGAACTTTGAAGTTTCATTTGATACTTGTCCTTTGGATAAGTAGTCAAACATTGTAGTGAATATGGGTAGCAATAGCACAACTGGAGGTGGAAAGTATGTTGGTTAAGTAAGAATTAGGTAGCAATAGCACAACTGGAAGTGGGAAGTATGTTGGTTAAGTAAGAATTAGGTAGCAATAGCAACAGGCAACAGCTACATTTCATGCCATGGTTACAGCTTTGCATGTTCAATTGGCTCTCTTGGACAGTTGT。
Wherein, the amplified band type of the maize inbred line W22 is an excellent allele for reducing the spike height. Therefore, if the molecular weight of the PCR amplification product of the corn sample to be detected is 329bp, the corn sample to be detected contains alleles for reducing the corn ear height; if the molecular weight of the PCR amplified product of the corn sample to be detected is 277bp, the corn sample to be detected contains alleles for increasing the height of corn ears.
The alignment results of SEQ ID No.3 and SEQ ID No.4 are shown in FIG. 1. The electrophoresis results of PCR amplified products of maize inbred line W22 and maize wild-type inbred species CIMMYT8759 are shown in FIG. 2.
Example 2: method for obtaining molecular marker EH1
The method for obtaining the molecular marker EH1 specifically comprises the following steps:
step 1: construction of BC containing 866 families 2 S 3 Population of introgression lines
Corn inbred line W22 is used as an acceptor parent, corn wild inbred line CIMMYT8759 is used as a donor parent, and BC containing 866 families is obtained through hybridization for 1 generation, backcross for 2 generation and selfing for 3 generation 2 S 3 The introgression line population.
Step 2: field planting and phenotyping of introgressed populations
BC is planted in spring 2019 at a national crop variety area test station in Liuyang City (28.2N, 113.6E) of Hunan province 2 S 3 The introgression line population. The field test adopts an amplified incomplete random granule design. 2 rows of plants are planted in each district, 15 plants are planted in each row, and the plant spacing is 25cm. 2 families are planted in each ridge. The ridge height is 15cm, the ridge width is 70cm, and the ditch width is 30cm.
The ear height phenotype was measured 10 days after powder dispersion, and 8 individuals were continuously investigated for each family starting from strain 3. The spike height is the height from the ground to the female spike node.
Step 3: performing QTL positioning analysis
QTL positioning analysis was performed using the multi QTL model of R/QTL. Firstly, performing simple interval positioning analysis on the QTL by using Haley-Knott regression, and determining the LOD threshold value (alpha=0.05) of the spike height QTL by using a method of substitution test 10000 times. And (3) carrying out multi-QTL model fitting on the QTL model obtained by positioning the simple interval, and optimizing the position of each QTL by utilizing a refiequtl command of R/QTL. Further using addqtl command to detect if there are other QTLs of the genome that significantly improve the model, if a new QTL is detected, re-fitting the multi-QTL model and optimizing QTL positions, repeating this process until no new QTL is detected. Finally, calculating the total surface variation of all QTL interpretations and the additive effect and phenotype contribution rate of the single QTL by using the fitqtl command.
QTL positioning result analysis: a total of 10 QTL's controlling the ear position height were detected, one QTL qEH1-2 with a greater phenotypic effect being detected on chromosome 1. qEH1-2 has a LOD of 12.84, an additive effect of 6.25cm, a dominant effect of 2.93cm, and a phenotype contribution of 4.71% and is located in the region 232529138bp to 237965521bp of chromosome 1 in maize.
Step 4: development and synthesis of molecular marker EH1
The primer3 (https:// primer3.Ut. Ee /) is used to search qEH-2 in the physical range of 232529138bp to 237965521bp of chromosome 1, and the forward amplification primer EH1-F and the reverse amplification primer EH1-R are designed, and the primers are synthesized by Beijing qing biological science and technology Co., ltd, and the nucleotide sequence is as follows:
forward amplification primers EH1-F:5'-ACAGTGCCAGCTTTGATGTT-3', as shown in SEQ ID No. 1;
reverse amplification primer EH1-R:5'-ACAACTGTCCAAGAGAGCCA-3' as shown in SEQ ID No. 2.
Example 3: application of molecular marker EH1
Introgression line MR0919, which is heterozygous only in the qEH1-2 region and homozygous at the other genomic locus, is used as starting material and self-pollinated to generate an F isolated only in the qEH1-2 region 2 A population. To contain F of 324 individual plants 2 The colony is a material for verifying the molecular marker EH1 obtained by the invention so as to determine the accuracy of the molecular marker applied to molecular marker assisted selective breeding. The method specifically comprises the following steps:
step 1: f (F) 2 Determination of population spike height
Determination of F according to the procedure of example 2 2 The ear position of the plant of the colony is high.
Step 2: the DNA of corn leaves is extracted by adopting a CTAB method.
Step 3: PCR amplification
The reaction system for PCR amplification was 10. Mu.L, including:
(1) 1. Mu.L of forward amplification primer shown in SEQ ID No.1 at a concentration of 10. Mu.m 1/L;
(2) 1. Mu.L of the reverse amplification primer shown in SEQ ID No.2 at a concentration of 10. Mu.m 1/L;
(3) 1. Mu.L of DNA template at a concentration of 100 ng/. Mu.L;
(4) 5 μL 2X Taq PCR StarMix with Loading Dye (Beijing Kang Runcheng Biotechnology Co., ltd., product number: A012-01);
(5)2μL ddH 2 O。
the procedure for PCR amplification was as follows:
(1) Pre-denaturation at 95℃for 10min;
(2) Denaturation at 95℃for 45s, annealing at 58℃for 45s, elongation at 72℃for 60s,35 cycles;
(3) Extending at 72 ℃ for 10min;
(4) Preserving at 4 ℃.
PCR instrument model: eppendorf Mastercycler nexus.
Step 4: electrophoresis
Molecular marker EH1 in part F 2 The electropherogram of PCR amplified products in the individual plants is shown in FIG. 3.
Step 5: analysis of results
Determining the genotype of the corn sample to be detected according to the molecular weight of the PCR amplification product: if the PCR amplified product of the corn sample to be tested has only one 329bp band, the corn to be tested is homozygous for the W22 genotype (i.e., the genotype identical to the corn inbred W22 genotype); if the PCR amplified product of the corn sample to be detected has only one 277bp band, the corn to be detected is homozygous CIMMYT8759 genotype (namely the genotype same as the wild kindred species CIMMYT8759 of corn); if the PCR amplified product of the corn sample to be detected has not only a 329bp band but also a 277bp band, the corn to be detected is heterozygous genotype.
F 2 The total 73 strains of the single strain are homozygous W22 genotypes, and the PCR products are sequenced and displayedThe sequences are shown as W22 genotype F homozygous for SEQ ID No.3, 73 strains 2 The height of the ear position of the single plant is 50.1+/-9.5 cm; the sequence of PCR products is shown to be SEQ ID No.4, and the sequence of the PCR products is shown to be the genotype F of the 79 strain homozygous CIMMYT 3535 2 The height of the ear position of the single plant is 62.8+/-12.9 cm; the total 172 strains are heterozygous genotypes, and sequencing of PCR products shows that the sequences of the 172 strains are SEQ ID No.3 and SEQ ID No.4, 172 heterozygous genotypes F 2 The height of the spike of the single plant is 53.3+/-8.7 cm.
Analysis of variance was further performed on the spike height phenotype values for each group (fig. 4). The results show that: homozygous W22 genotype F 2 The spike height of the single plant is extremely lower than that of homozygous CIMMYT8759 genotype F 2 Single, homozygous W22 genotype F 2 The ear height of the single plant is obviously lower than that of heterozygous genotype F 2 Single plant, heterozygous genotype F 2 The spike height of the single plant is extremely lower than that of homozygous CIMMYT8759 genotype F 2 The single plant shows that the molecular marker EH1 is related to the ear position height of corn, and has important breeding application value.
In conclusion, the molecular marker EH1 and qEH1-2 provided by the invention are tightly linked, so that the corn ear position can be rapidly and accurately identified, the application of the locus in the breeding of new corn varieties can be promoted, and the molecular polymerization breeding of the locus and other excellent character loci can be facilitated. The method provided by the invention can identify and screen corn germplasm resources at any stage of corn, has the advantages of simplicity, convenience, rapidness, high efficiency and accuracy, and is suitable for large-scale popularization and application.
The present invention is described in detail above. It will be apparent to those skilled in the art that the present invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with respect to specific embodiments, it will be appreciated that the invention may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The application of some of the basic features may be done in accordance with the scope of the claims that follow.
Claims (8)
1. The application of the substance for detecting the corn cob position high molecular marker in detecting or assisting in detecting the corn cob position high character is that the corn cob position high molecular marker is a DNA fragment shown as SEQ ID No.3 and a DNA fragment shown as SEQ ID No.4.
2. The use according to claim 1, characterized in that: the substance for detecting the corn ear position high molecular marker is a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table.
3. A method for detecting a high ear position trait of maize comprising: the genome DNA of corn to be detected is used as a template, a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table is utilized for PCR amplification, the ear position of the obtained homozygous corn with the PCR product sequence of SEQ ID No.3 is lower than or candidate lower than that of the obtained homozygous corn with the PCR product sequence of SEQ ID No.4, the ear position of the obtained homozygous corn with the PCR product sequence of SEQ ID No.3 is lower than or candidate lower than that of the obtained heterozygous corn with the PCR product sequences of SEQ ID No.3 and SEQ ID No.4, and the ear position of the obtained heterozygous corn with the PCR product sequences of SEQ ID No.3 and SEQ ID No.4 is lower than or candidate lower than that of the obtained homozygous corn with the PCR product sequence of SEQ ID No.4.
4. A method for detecting a high ear position trait of maize comprising: the genome DNA of corn to be detected is used as a template, and PCR amplification is carried out by using a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table, the ear height of the obtained homozygous corn with the size of 329bp is lower than or lower than that of the obtained homozygous corn with the size of 277bp, the ear height of the obtained homozygous corn with the size of 329bp is lower than or lower than that of the obtained heterozygous corn with the size of 329bp and 277bp, and the ear height of the obtained heterozygous corn with the size of 329bp and 277bp is lower than or lower than that of the obtained homozygous corn with the size of 277 bp.
5. A method of maize breeding comprising: and (3) taking genome DNA of the corn to be detected as a template, carrying out PCR amplification by using a primer pair consisting of two single-stranded DNA shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table, and selecting the corn to be detected with a PCR product of SEQ ID No.3 as a parent to finish breeding.
6. The use of the substance for detecting the high molecular marker in the corn cob position as claimed in claim 1 or 2 for preparing a product for detecting the high character in the corn cob position.
7. The use of the maize ear position polymer marker according to claim 1 or 2 for detecting or assisting in detecting maize ear position high traits.
8. The use of the maize ear position polymer marker of claim 1 or 2 in maize breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210888929.7A CN116004881A (en) | 2022-07-27 | 2022-07-27 | Molecular marker highly closely linked with corn ear position and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210888929.7A CN116004881A (en) | 2022-07-27 | 2022-07-27 | Molecular marker highly closely linked with corn ear position and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116004881A true CN116004881A (en) | 2023-04-25 |
Family
ID=86019776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210888929.7A Pending CN116004881A (en) | 2022-07-27 | 2022-07-27 | Molecular marker highly closely linked with corn ear position and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116004881A (en) |
-
2022
- 2022-07-27 CN CN202210888929.7A patent/CN116004881A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10959386B2 (en) | Melon plants with improved disease tolerance | |
| US11032986B2 (en) | Methods of creating drought tolerant corn plants using markers linked to cold shock domain-containing proteins and compositions thereof | |
| CN109337998B (en) | InDel6 and SSR229 molecular marker development method closely linked with corn plant height and application thereof | |
| CN112795692B (en) | Molecular marker linked with corn plant height and application thereof | |
| CN112795691B (en) | Molecular marker linked with corn stem thickness and application thereof | |
| US20230250446A1 (en) | Tomato plants with resistance to mi-1 resistance-breaking root-knot nematodes | |
| CN109486829B (en) | Rice semi-dwarf gene sd1 allele and identification method thereof | |
| CN112795693B (en) | Molecular marker related to chlorophyll content of corn leaf and application thereof | |
| US10590491B2 (en) | Molecular markers associated with Mal de Rio Cuarto Virus in maize | |
| US10028459B2 (en) | Tomato plants with improved disease resistance | |
| US20210324395A1 (en) | Tomato plants with improved traits | |
| AU2014268142A1 (en) | Disease resistance loci in onion | |
| CN115820897B (en) | Molecular marker closely linked with corn female spike and sword leaf length and application thereof | |
| CN115927704B (en) | Molecular marker closely linked with length of corn stalk and application thereof | |
| US11319554B2 (en) | Cucumber mosaic virus resistant pepper plants | |
| CN115820895B (en) | Molecular marker closely linked with chlorophyll content of corn and application thereof | |
| US10172305B2 (en) | Diagnostic molecular markers for seed lot purity traits in soybeans | |
| CN116004881A (en) | Molecular marker highly closely linked with corn ear position and application thereof | |
| CN109207621A (en) | The molecular labeling and its methods and applications of Stigma Extrusion of Rice gene qSE7 | |
| US9725773B2 (en) | Molecular markers associated with green snap in maize | |
| CN115927704A (en) | Molecular marker closely linked with length of corn stalk and application thereof | |
| CN115820895A (en) | Molecular marker closely linked with chlorophyll content of corn and application thereof | |
| CN115896323A (en) | Molecular marker closely linked with germination capacity of corn seeds and application thereof | |
| US20140259232A1 (en) | Molecular markers associated with earliness in maize |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |