CN115997791A - Application of Burkholderia cepacia in promoting saline-alkali tolerance of plants and reducing heavy metal cadmium content - Google Patents
Application of Burkholderia cepacia in promoting saline-alkali tolerance of plants and reducing heavy metal cadmium content Download PDFInfo
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention discloses application of Burkholderia cepacia in promoting saline-alkali tolerance of plants and reducing heavy metal cadmium content. The research of the invention shows that Burkholderia cepacia JT79 strain has good salt and alkali resistance and capability of reducing the content of heavy metal cadmium, and can normally grow in a high-salt-alkali and cadmium-containing culture medium; JT79 strain can obviously improve the capability of soybean and corn for resisting saline-alkali stress, promote the growth and development of soybean and corn, and simultaneously can efficiently reduce the cadmium content. The JT79 bacteria have the functions of salt and alkali resistance, growth promotion, cadmium resistance and cadmium content reduction, can effectively relieve the condition that the plant nutrition growth process is inhibited under the stress of salt and alkali, can prevent and treat the heavy metal cadmium pollution of soil, improve the salt and alkali resistance and the cadmium resistance of plants, and promote the plant growth. The invention provides a new method and thinking for the utilization of saline-alkali soil, prevention and treatment of soil cadmium toxicity and reduction of cadmium content, and provides excellent strain resources with better saline-alkali resistance, cadmium resistance and growth promotion.
Description
Technical Field
The invention belongs to the technical field of agricultural microorganisms. More particularly relates to application of Burkholderia cepacia in promoting saline-alkali tolerance of plants and reducing the content of heavy metal cadmium.
Background
Soil salinization refers to the phenomenon or process that soluble salt accumulates on the surface layer of soil, and is expressed as that the content of organic matters in the soil is relatively reduced, so that the soil nutrition condition is worsened, and the microbial activity of the soil is inhibited by the salt and the alkali. On one hand, the harm of the saline alkali to the crops is that the physical and chemical properties of the soil are indirectly changed, so that the plants lose good living environment and nutrition conditions; on the other hand, the soil solution directly damages crop cells to influence the normal absorption and metabolism functions of crops. The salinization soil environment is extremely easy to cause the crop root system to dehydrate due to the too high outside osmotic pressure, even corrode the root system, and accelerate the death of the root system, thereby causing the crop to wither in a sheet, the particles are not harvested, and only a few saline-alkali tolerant crops can survive in the saline-alkali soil.
The problem of environmental pollution by heavy metal cadmium has begun to appear in the 20 th century following electrolytic zinc production, and the main sources of cadmium pollution in soil are waste water, waste gas and waste residues of mining, smelting, electroplating and basic chemical industries, and the application of cadmium-containing fertilizers, pesticides and agricultural sludge is also an important source of cadmium pollution in soil. And soil ecological problems such as soil hardening, soil heavy metal pollution, water eutrophication and the like are more serious due to long-term application of chemical fertilizers. Meanwhile, a large amount of chemical pesticides are used for preventing and controlling various diseases, so that the resistance of plant pathogenic bacteria is stronger and stronger, and serious problems such as pesticide residues are caused.
At present, in the environment pollution caused by the alleviation of plant salinization stress and the prevention and treatment of heavy metal cadmium, besides adopting chemical agents to neutralize salt and alkali, the method can transform and adsorb the heavy metal cadmium in soil, and can also utilize microorganisms to repair and prevent and treat, thereby having sustainability and environmental friendliness. Burkholderia cepacia is used as a novel excellent biocontrol fungus, can secrete various disease-preventing and growth-promoting substances, and has obvious effects on preventing and treating various diseases and promoting the growth of various plants. The domestic patent reports that Burkholderia cepacia has great development potential in plant growth promotion and biological control. For example, chinese patent CN112625970A discloses Burkholderia cepacia which can stably colonize soil and can prevent and treat more than ten diseases such as verticillium cotton and colletotrichum gloeosporioides; however, burkholderia cepacia has a fresh report on the effect of plants on relieving soil saline-alkali stress and preventing and controlling heavy metal cadmium from polluting the environment, and a microbial strain capable of being used for relieving soil saline-alkali stress and preventing and controlling heavy metal cadmium from polluting the environment is also lacking at present. In the research of soil salinization resistance and prevention of heavy metal cadmium on environmental pollution of plants, the screening of microorganism strains resistant to saline and alkaline and cadmium is necessary, an effective means is provided for the reduction of the content of cadmium in soil and the saline and alkaline-resistant hypochondriac area of plants, more excellent strain resources are provided, and the method has great significance for the treatment of soil cadmium poison.
Disclosure of Invention
The invention provides application of Burkholderia cepacia in promoting saline-alkali tolerance of plants and reducing heavy metal cadmium content.
Another object of the invention is to provide a method for promoting the resistance of plants to salt and alkali stress.
It is yet another object of the present invention to provide a method for reducing cadmium content of a sample.
The above object of the present invention is achieved by the following technical scheme:
the invention provides a new application of Burkholderia cepacia (Burkholderia cepacia) JT79 strain which is preserved in the microorganism strain collection of Guangdong province in 9 and 30 days of 2019, wherein the preservation number is GDMCC NO:60798, the preservation address is No. 100 of the Guangzhou City first-violent middle road. Researches show that Burkholderia cepacia JT79 strain has good salt and alkali resistance and capability of reducing the content of heavy metal cadmium, and can normally grow in a high-salt-alkali culture medium containing heavy metal cadmium; and JT79 strain can obviously improve the capability of soybean and corn for resisting salt and alkali stress, promote the growth and development of soybean and corn, and show that JT79 strain can effectively relieve the condition that the plant nutrition growth process is inhibited under the salt and alkali stress, improve the salt and alkali tolerance of the plant and promote the plant growth. Meanwhile, the cadmium content of the cadmium-containing culture medium can be reduced by more than 80% by adopting the JT79 strain to treat the cadmium-containing culture medium for 72 hours, which shows that the JT79 strain has higher reducing effect on the cadmium toxicity of soil, can be used for preventing and treating the heavy metal cadmium pollution of the soil, and improves the cadmium resistance of the plant.
Thus, the following applications are within the scope of the present invention:
application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in promoting saline-alkali stress resistance of plants or reducing cadmium content.
Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in preparing a preparation for improving the salt and alkali resistance of plants or a preparation for reducing the cadmium content.
Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in preventing and treating soil heavy metal cadmium pollution.
Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in promoting plant growth under saline-alkali stress.
Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in preparing saline-alkali tolerance and/or growth promoting agent.
The invention provides a method for promoting plants to resist saline-alkali stress, which adopts Burkholderia cepacia JT79 strain and/or bacterial liquid thereof to treat plants.
Further, the bacterial liquid is fermentation liquid.
The preparation method of Burkholderia cepacia JT79 fermentation broth provided by the invention comprises the following steps: and (3) inoculating single bacterial colonies after the bacterial strains are activated into an LB culture medium, culturing at 30 ℃ for 48 hours to obtain seed liquid, mixing the seed liquid with a liquid culture medium, and culturing for 48 hours to obtain the strain.
Preferably, the formula of the LB medium is as follows: yeast extract 5g, tryptone 10g, nacl5g, sterile water to 1L.
Preferably, the liquid medium is LB medium or NB medium.
More preferably, the formula of the LB medium is as follows: 10g of tryptone, 5g of yeast extract, 10g of NaCl, and constant volume of sterile water to 1L; the formula of the NB medium is as follows: beef extract 3g, peptone 10g, naCl5g, sterile water to 1L.
Preferably, the volume ratio of the seed liquid to the liquid culture medium is 1:10.
preferably, the concentration of the fermentation broth is not less than 2X 10 8 cfu/mL。
Preferably, the fermentation broth is diluted 5 to 50 times for use.
More preferably, the fermentation broth is diluted 25-fold for use.
Preferably, the treatment method is root irrigation.
Preferably, the plant is soybean, corn.
The invention also discloses a method for reducing the cadmium content of the sample, which adopts Burkholderia cepacia JT79 and/or bacterial liquid and fermentation liquid to treat the cadmium-containing culture liquid.
The invention has the following beneficial effects:
the invention provides a new application of Burkholderia cepacia (Burkholderia cepacia) JT79 strain, and researches show that the Burkholderia cepacia JT79 strain has good saline-alkali resistance and heavy metal cadmium resistance, and can normally grow in a high-saline-alkali and cadmium-containing culture medium; and the JT79 strain can effectively prevent and treat the damage of high salt and alkali to crops, promote the growth and development of the crops, remarkably improve the capability of the soybeans and the corns for resisting the salt and alkali stress, promote the growth and development of the soybeans and the corns, and increase the plant height, the lateral root number, the fresh weight of the overground part and the fresh weight of the underground part. The JT79 strain can effectively relieve the condition that the plant nutrition growth process is inhibited under the saline-alkali stress, improve the saline-alkali resistance of the plant and promote the plant growth. Meanwhile, the JT79 strain has the effect of obviously reducing the cadmium content, and the cadmium content can be reduced by more than 80% by adopting JT79 bacterial liquid and 25 times of diluent thereof to treat a cadmium-containing sample for 72 hours, which indicates that the JT79 strain has the efficient reducing effect on the cadmium content of the sample, can be used for preventing and treating heavy metal cadmium pollution of soil and improves the cadmium resistance of plants. The invention provides a new method and thinking for the utilization of saline-alkali soil and the prevention and treatment of the environmental pollution caused by heavy metal cadmium, and provides excellent strain resources which have better saline-alkali resistance and cadmium resistance and can promote growth for production selection.
Drawings
FIG. 1 shows colony morphology of JT79 strain on saline-alkali containing medium.
FIG. 2 shows the effect of JT79 on soybean salt and alkali stress (A: plant height, B: fibrous root number, C: fresh weight of the ground, D: fresh weight of the ground, CK: fresh water treatment, SA: salt and alkali treatment, SA+Bc: salt and alkali treatment plus JT79 fermentation broth, SA+Bc×5: salt and alkali treatment plus 5-fold dilution of JT79 fermentation broth, SA+Bc×25: salt and alkali treatment plus 25-fold dilution of JT79 fermentation broth, SA+Bc×50: salt and alkali treatment plus 50-fold dilution of JT79 fermentation broth).
FIG. 3 shows the effect of JT79 on corn salt and alkali stress (A: plant height; B: side root number; C: fresh weight on the ground; D: fresh weight on the ground; CK: clean water treatment; SA: salt and alkali treatment; SA+Bc: salt and alkali treatment plus JT79 fermentation broth; SA+Bc×5: salt and alkali treatment plus 5-fold dilution of JT79 fermentation broth; SA+Bc×25: salt and alkali treatment plus 25-fold dilution of JT79 fermentation broth; SA+Bc×50: salt and alkali treatment plus 50-fold dilution of JT79 fermentation broth).
FIG. 4 shows the effect of JT79 cell suspension on soybean salt and alkali stress (CK: clear water treatment, SA: salt and alkali treatment, SA+Bc (Solution): salt and alkali treatment plus JT79 fermentation broth, SA+Bc (Thalus): salt and alkali treatment plus JT79 cell suspension).
Fig. 5 is a graph of the pro-effect of JT79 cell suspension on soybean salt and alkali stress (non-salt and alkali treatment, salt and alkali + cell treatment in sequence from left to right).
FIG. 6 is a graph showing the results of a tolerance test of JT79 strain on a cadmium-containing medium.
FIG. 7 shows the results of cadmium content measurements before and after JT79 fermentation broth treatment.
Detailed Description
The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
Reagents and materials used in the following examples are commercially available unless otherwise specified.
Example 1 preparation of Burkholderia cepacia JT79 fermentation broth
The burkholderia cepacia (Burkholderia cepacia) JT79 strain used in this example was a subject group-keeping strain of the present invention, which was deposited at the microorganism strain collection in guangdong province at 9/30/2019 under the deposit number GDMCC NO:60798, the preservation address is No. 100 of the Guangzhou City first-violent middle road.
Activating strain preserved at-80deg.C, picking activated colony, culturing in LB liquid medium (yeast extract 5g, tryptone 10g, naCl5g, water 1000mL, pH 7.2) at 121deg.C for 15min, and culturing at 30deg.C for 48 hr to obtain seed solution.
According to the content of onion Burkholderia cepaciaThe seed solution containing Burkholderia cepacia JT79 and the liquid culture medium are added into NB liquid culture medium (beef extract 3g, peptone 10g, naCl5g, and sterile water to fix volume to 1L) according to the volume ratio of 1:10, and cultured for 48 hours at 30 ℃ and 180r/min to obtain Burkholderia cepacia JT79 fermentation broth. Wherein the fermentation broth concentration of Burkholderia cepacia JT79 is 2×10 8 cfu/mL。
Example 2 Burkholderia cepacia JT79 saline-alkaline medium tolerance test
1. Preparation of saline-alkali culture medium
NA solid saline-alkali medium: beef extract 3g, peptone 10g, agar 20g, 0.06mM saline-alkali, pH 9.0, adding sterile water to 1L, sterilizing at 121deg.C for 15min.
2. Test treatment
After fermentation broth containing Burkholderia cepacia JT79 is diluted properly on an ultra-clean workbench, 200 mu L of fermentation broth is taken, uniformly spread on an NA solid saline-alkali flat plate by a spreading rod, after the fermentation broth is completely dried in the shade, the flat plate is sealed by a sealing film, the flat plate is placed in a room temperature incubator, colony growth conditions are observed and recorded every day, and the NA solid culture medium is used as a control.
3. Analysis of results
As shown in FIG. 1, burkholderia cepacia JT79 has good saline-alkali tolerance, and can normally grow under saline-alkali condition with pH of 0.06mM and pH of 9.0.
Example 3 anti-saline-alkaline stress growth promoting Effect of Burkholderia cepacia fermentation broth on soybeans
15 soybean seedlings with the same growth vigor are cultivated and grown to 4 leaves, then the leaves are transferred into a potting container with the diameter of 6cm and the height of 12cm, 100g of matrix soil is added for planting, and the seedlings are treated after fixed planting for 2 days. Five experimental treatments were set up, 3 replicates each:
treatment one: non-saline treatment, and pouring 20mL of sterile water.
And (2) treatment II: saline treatment, 10mL saline solution (0.06 mM, pH 9.0) and 10mL sterile water were added.
And (3) treatment III: saline-alkali + bacterial solution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) was added, and 10mL JT79 fermentation broth was added.
And (4) treatment four: saline-alkali + bacterial solution dilution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) and 10mL JT79 5-fold diluted bacterial solution are added.
And (5) treatment: saline-alkali + bacterial solution dilution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) and 10mL JT79 of 25-fold diluted bacterial solution are added.
And (3) treatment six: saline-alkali + bacterial solution dilution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) and 10mL JT79 of 50-fold diluted bacterial solution are added.
The plants treated by each group are placed in a greenhouse for cultivation, and the plants are treated 1 time every 7 days, and after the cultivation is completed for 30 days, the plant height, the side root number, the fresh weight of the overground part and the fresh weight of the underground part of the plants are measured.
As shown in fig. 2, the plant height, the side root number and the fresh weight of the ground and underground parts of the soybeans are reduced by 60.23%,66.71%,40.52%,50.23% respectively under the saline-alkali treatment, and the difference is remarkable compared with the first control treatment. Under the stress of soybean salt and alkali, the plant height of the soybeans is respectively increased by 3.71%,81.82%,20.37% and 64.81% by adding JT79 fermentation liquor stock solution and 5 times, 25 times and 50 times of diluent solution; the lateral root number is increased by 30.31%,42.42%,96.97% and 81.82% respectively; the fresh weight of the overground part is increased by 48.38 percent, 33.79 percent, 38.63 percent and 49.66 percent respectively; the fresh weight of the underground part is respectively increased by-8.9%, 23.64%,47.13% and 61.97%, which shows that the addition of the fermentation liquor of Burkholderia cepacia JT79 can relieve the saline-alkali stress of soybeans, thereby promoting the growth of the soybeans.
Example 4 anti-saline stress growth promoting Effect of Burkholderia cepacia fermentation broth on corn
15 maize seedlings with the same growth vigor are cultivated and grown to 4 leaves, then the leaves are transferred into a potting container with the diameter of 6cm and the height of 12cm, 100g of matrix soil is added for planting, and the seedlings are treated after being subjected to field planting for 2 days. Five experimental treatments were set up, 3 replicates each:
treatment one: non-saline treatment, and pouring 20mL of sterile water.
And (2) treatment II: saline treatment, 10mL saline solution (0.06 mM, pH 9.0) and 10mL sterile water were added.
And (3) treatment III: saline-alkali + bacterial solution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) is added, and 10mL JT79 bacterial solution is added.
And (4) treatment four: saline-alkali + bacterial solution dilution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) and 10mL JT79 5-fold diluted bacterial solution are added.
And (5) treatment: saline-alkali + bacterial solution dilution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) and 10mL JT79 of 25-fold diluted bacterial solution are added.
And (3) treatment six: saline-alkali + bacterial solution dilution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) and 10mL JT79 of 50-fold diluted bacterial solution are added.
The plants treated by each group are placed in a greenhouse for cultivation, and are treated 1 time every 7 days, and after the cultivation is completed for 30 days, the plant height, the side root number, the fresh weight of the overground part and the fresh weight of the underground part of the plants are measured.
The experimental results are shown in fig. 3, and compared with the control treatment, the plant height, the lateral root number and the fresh weight of the overground and underground parts of the corn are reduced by 40% in the saline-alkali treatment, and the saline-alkali condition seriously inhibits the growth of the corn. After the corn is treated by saline and alkaline, adding JT79 fermentation liquor stock solution and 5 times, 25 times and 50 times of diluent for treatment, the plant height of the corn is respectively increased by 0%,47.06%,58.82% and 11.76%; the number of the side roots is respectively increased by 2, 4 and 1; the fresh weight of the overground part is respectively increased by 19.67%,87.83%,133.35% and 31.65%; the fresh weight of the underground part is increased by-7.39%, 28.24%,72.36% and 11.82% respectively. Wherein, the growth promoting effect of the bacterial liquid after 25 times dilution is optimal, and under the treatment of JT79 fermentation liquid after 25 times dilution, the plant height, the lateral root number and the fresh weight of the overground and underground parts are respectively 13.5cm,4 fibrous roots, 0.3275g and 0.4996g. The difference from the treatment one is not significant.
EXAMPLE 5 stress-resistant growth-promoting Effect of JT79 cell suspension
After 12 soybean seedlings with the same growth vigor are cultivated and grown to 4 leaves, the soybean seedlings are transferred into a potting container with the diameter of 6cm and the height of 12cm, 100g of matrix soil is added for planting, and the soybean seedlings are treated after being subjected to field planting for 2 days.
The preparation method of JT79 thallus suspension comprises the following steps: 100mL JT79 fermentation broth is taken and separated by a 50mL sterile centrifuge tubeAfter loading, the mixture was centrifuged at 6000rpm for 10min. Removing supernatant, adding equal amount of sterile water to wash thallus, reversing to obtain bacterial suspension, and centrifuging at 6000rpm for 10min. Removing supernatant, adding 10mL of sterile water into the bacterial precipitate, resuspending, and measuring OD with spectrophotometer 600 Value, adjust to OD 600 =1.0. Five experimental treatments were set up, 3 replicates each:
treatment one: non-saline treatment, and pouring 20mL of sterile water.
And (2) treatment II: saline treatment, 10mL saline solution (0.06 mM, pH 9.0) and 10mL sterile water were added.
And (3) treatment III: saline-alkali + bacterial solution treatment, 10mL saline-alkali solution (0.06 mM, pH 9.0) was added, and 10mL JT79 fermentation broth was added.
And (4) treatment four: saline and bacteria treatment, 10mL saline solution (0.06 mM, pH 9.0) was added, followed by 10mL JT79 bacteria suspension.
After each group of treatments, the treatment was performed 1 time every 7 days, and after the cultivation was completed for 30 days, the plant height, the number of side roots, the fresh weight of the above-ground part and the fresh weight of the below-ground part were measured.
The experimental result is shown in fig. 4, the plant height of the control group soybean is 14.3 cm, the plant height in the saline-alkali treatment is 4.3 cm, and the plant height of the control group soybean is 30%, and the difference between the two is obvious; the plant height after JT79 bacteria liquid treatment is 13.8 cm, which is 96.5% of the plant height of a control group, and the difference is not obvious; the plant height after JT79 thallus treatment is 11 cm, which is 76.9% of the plant height of the control group, and the difference is not obvious. The effect of JT79 bacteria liquid on promoting soybean saline-alkali stress is shown in figure 5, which shows that the effect of JT79 fermentation liquid on promoting plant growth under saline-alkali stress is superior to JT79 bacteria suspension, and the effect of promoting plant growth is equivalent to that of control group under normal condition.
Example 6 Burkholderia cepacia JT79 cadmium-containing Medium tolerance test
1. Preparation of cadmium-containing culture medium
NA solid cadmium-containing culture medium (15 mg cadmium chloride, beef extract 3g, peptone 10g, naCl5g, agar 20g, adding sterile water to volume to 1L, sterilizing at 121deg.C for 15 min.)
2. Test treatment
After the seed solution containing Burkholderia cepacia JT79 is properly diluted on an ultra-clean workbench, 200 mu L of the seed solution is taken, the seed solution is uniformly spread on an NA solid cadmium-containing flat plate by a coating rod, the flat plate is sealed by a sealing film after the seed solution is completely dried in the shade, the flat plate is placed in a room temperature incubator, and the colony growth condition is observed and recorded every day and is used as a control by using the NA solid culture medium.
3. Analysis of results
As shown in FIG. 6, burkholderia cepacia JT79 has good cadmium resistance and can normally grow in an environment with a cadmium content of 15 mg/L.
Example 7 test of cadmium reduction in Burkholderia cepacia JT79 cadmium-containing Medium
1. Preparation of cadmium-containing culture medium
NB liquid cadmium-containing medium (15 mg cadmium chloride, beef extract 3g, peptone 10g, naCl5g, adding sterile water to 1L, sterilizing at 121deg.C for 15 min).
2. Test treatment
Adding the seed solution containing Burkholderia cepacia JT79 into NB liquid cadmium-containing medium respectively according to the volume ratio of the seed solution containing Burkholderia cepacia JT79 to the liquid medium of 1:10, and culturing at 30 ℃ and 180r/min for 72h and 96h respectively to obtain a fermentation broth containing cadmium of Burkholderia cepacia JT 79. In this experiment, a cadmium-containing (5 mg/L) NB medium was used as a control, and fermentation broth (concentration: 2X 10) containing Burkholderia cepacia JT79 was added 8 cfu/mL) of the treated medium was the experimental group, and each treatment was repeated 5 times. JT79 strain fermentation broth prepared in example 1 was used. After the culture mediums are respectively cultured for a certain time, respectively sampling, taking a certain amount of fermentation liquor, obtaining 10mL of supernatant by high-speed centrifugation (1000 r/min), and then carrying out filtration sterilization (500 meshes) on the supernatant to carry out cadmium-containing measurement.
3. Analysis of results
As a result, as shown in FIG. 7, the cadmium concentration of the control group reached 15.51mg/L, and the cadmium concentration in the solutions after 72 and 96 hours of treatment with JT79 strain fermentation broth was 0.92mg/L and 0.75mg/L, respectively. The reduction rate of the cadmium content in the solution exceeds 90%, which shows that the JT79 strain has passivation or enrichment effect on the cadmium and has remarkable effect.
In conclusion, burkholderia cepacia JT79 strain has good salt and alkali resistance and capability of reducing the content of heavy metal cadmium, can normally grow in a high-salt-alkali culture medium and a cadmium-containing environment, and has good salt and alkali resistance and cadmium resistance; and the JT79 strain can effectively prevent and treat the damage of high salt and alkali to crops, promote the growth and development of the crops, remarkably improve the capability of the soybeans and the corns for resisting the salt and alkali stress, promote the growth and development of the soybeans and the corns, and increase the plant height, the lateral root number, the fresh weight of the overground part and the fresh weight of the underground part. The JT79 strain can effectively relieve the condition that the plant nutrition growth process is inhibited under the saline-alkali stress, improve the saline-alkali resistance of the plant and promote the plant growth. Meanwhile, the cadmium content of the sample containing cadmium can be reduced by more than 80% by adopting JT79 bacterial liquid and 10 times of diluent for 72 hours, which shows that JT79 bacterial strain has high-efficiency reduction effect on the cadmium content of the sample. The invention provides a new method and thinking for the utilization of saline-alkali soil and the prevention and the utilization of cadmium metal contaminated soil, and researches and obtains excellent strain resources which have better saline-alkali tolerance and living promotion and can be used for the prevention and the treatment of heavy metal contaminated soil for production selection.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (10)
1. Application of Burkholderia cepacia (Burkholderia cepacia) JT79 strain and/or bacterial liquid thereof in promoting saline-alkali stress resistance of plants or reducing cadmium content.
2. Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in preparing a preparation for improving the salt and alkali resistance of plants or a preparation for reducing the cadmium content.
3. Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in preventing and treating soil heavy metal cadmium pollution.
4. Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in promoting plant growth under saline-alkali stress.
5. Application of Burkholderia cepacia JT79 strain and/or bacterial liquid thereof in preparing saline-alkali tolerance and/or growth promoting agent.
6. A method for promoting the saline-alkali stress resistance of plants is characterized in that Burkholderia cepacia JT79 strain and/or bacterial liquid thereof are adopted for treating the plants.
7. The method according to claim 6, wherein the bacterial liquid is a fermentation liquid, and the concentration of the fermentation liquid is not lower than 2X 10 8 cfu/mL。
8. The method according to claim 7, wherein a fermentation broth diluted 5 to 50 times is used.
9. A method for reducing the cadmium content of a sample is characterized in that Burkholderia cepacia JT79 and/or a bacterial solution and a fermentation solution thereof are adopted to treat a cadmium-containing culture solution.
10. The use according to claims 1 to 5 or the method according to claim 6 or 9, wherein the burkholderia cepacia JT79 strain has been deposited at the cantonese collection of microorganism strains at 30/9/2019 under the deposit number GDMCC NO:60798, the preservation address is No. 100 of the Guangzhou City first-violent middle road.
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