CN115991768A - Neutrophil gelatinase-associated lipocalin (NGAL) detection kit - Google Patents
Neutrophil gelatinase-associated lipocalin (NGAL) detection kit Download PDFInfo
- Publication number
- CN115991768A CN115991768A CN202211126835.2A CN202211126835A CN115991768A CN 115991768 A CN115991768 A CN 115991768A CN 202211126835 A CN202211126835 A CN 202211126835A CN 115991768 A CN115991768 A CN 115991768A
- Authority
- CN
- China
- Prior art keywords
- ngal
- cancer
- antibody
- monoclonal antibody
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The invention relates to a neutrophil gelatinase-associated lipocalin (NGAL) detection kit. The invention provides a specific monoclonal antibody aiming at NGAL protein, and the antibody has better specificity and binding activity. The antibody is used for detecting NGAL protein, and has the characteristics of strong specificity, high sensitivity, short detection time and wide detection sample range; the detection method does not need any special instrument or equipment, and has the characteristic of low detection cost; the detection kit is simple and convenient to operate, and does not need to be operated by professionals; the kit disclosed by the invention is convenient to store and has a good application prospect.
Description
Technical Field
The application relates to the field of biological detection, in particular to a neutrophil gelatinase-associated lipocalin (NGAL) detection kit.
Background
Neutrophil gelatinase-associated lipocalin (NGAL) is one of lipocalins, initially a small molecular weight secreted protein found in activated neutrophils, and modern research has shown that NGAL is one of the most effective biological markers for diagnosing acute kidney injury, and also one of the effective markers for early diabetic nephropathy. NGAL has powerful functions, in addition to its ability to bind and transport hydrophobic small molecules as a member of the lipid-bearing family, and is associated with inflammation, embryonic development, immune response, chemotaxis, signal transduction, and the development and progression of a variety of tumors. NGAL has antiinflammatory, kidney progenitor cell differentiation promoting, N-cadherin repairing, heme oxygenase up-regulating, and cell death inhibiting effects.
It was found that serum, urinary NGAL levels in chronic kidney disease CKD patients were significantly higher than normal populations and were closely inversely related to Glomerular Filtration Rate (GFR), whereas sCr was less correlated with GFR than NGAL. Thus, NGAL levels in CKD patients are not only better indicators than sCr reflecting CFR decline, but are also markers for evaluating the extent of kidney damage in CKD patients. However, CKD patients have less elevated urinary NGAL than AKI patients and may be associated with interference from other chronic disease factors. A European adult patient cohort study for CKD found that patients with serum NGAL > 435ng/mL or urine NGAL > 231ng/mL reached follow-up endpoint events earlier; and the risk of the progression of the CKD is respectively improved by 3 percent and 2 percent when the concentration of the serum and urine NGAL is increased by 10ng/mL, and the serum and urine NGAL are independent predictors of the risk of the progression of the CKD through the analysis of a multivariate Cox proportional risk regression model.
NGAL has been demonstrated as a marker of acute kidney injury. Blood and urine NGAL concentrations are usually rapidly increased in early diagnosis of acute kidney function injury (AKI), the blood and urine NGAL concentrations are most obvious (tens to hundreds times higher than a critical value), and serum creatinine (sCr), urease and other traditional indexes are obviously increased after 24-72 hours, so NGAL can be used for early diagnosis of AKI. Mishra et al found in the queue study of infants with concurrent AKI by extracorporeal circulation that the sensitivity and specificity were 100% and 98% respectively when the critical value of AUCROC for 2h of urine NGAL diagnosis AKI was 50 μg/L (ELISA method) and that the sensitivity and specificity were 70% and 94% respectively when the critical value of AUCROC for serum NGAL diagnosis AKI was 0.906; multiple regression analysis showed that 2h urinary NGAL levels were a powerful predictor of predictive AKI. Adult patients were also found to have 2h urine, serum NGAL levels as a reliable early diagnostic indicator for diagnosis of AKI. meta analysis shows that NGAL has diagnostic efficacy on AKI affected by factors such as specimen type, age, detection method, etc., but NGAL has diagnostic efficacy on childhood AKI better than that of adults, who may be affected by other chronic diseases, etc. Notably, NGAL may not be suitable for early diagnosis of AKI due to factors such as inflammatory response in critically ill patients such as sepsis, and diagnosis specificity is poor. Studies in North America have found that serum NGAL diagnosis AKI of AUCROC 0.677, sensitivity 86% and specificity only 39% at a threshold of 139ng/mL, within 24h after admission of 143 systemic inflammatory response syndromes and septic shock infants. After multivariate analysis, blood NGAL was found not to be a predictor of the occurrence of AKI events. Similar conclusions were drawn in adult patient studies. Another study showed that urine NGAL levels in the critically ill infant within 48 hours could accurately predict the occurrence of AKI (AUCROC 0.79). But after sCr rises, AUCROC drops to 0.63. There was also no significant difference between plasma NGAL levels in ICU in AKI adult patients after 48h and normal control. Urine NGAL can be used as an early diagnostic marker for post-sepsis complicated AKI to an accuracy of 0.968, which may be related to inter-ethnic level differences.
In recent years, the function of the NGAL gene has been increasingly emphasized. Studies have shown that NGAL is intimately involved in the regulation and differentiation of bacterial or non-bacterial infectious diseases, some chronic inflammatory diseases, invasion and metastasis of tumors, and tumor cells. Moreover, the research results of NGAL and inflammation show that the expression characteristics of NGAL in the diseases tend to be mature, and the NGAL is very likely to become a marker for diagnosing various clinical inflammation-related diseases, and meanwhile, the functions and mechanisms of the NGAL in the invasion and metastasis processes of tumors tend to be clear, and the regulation and differentiation of the NGAL and tumor cells become the turning points of people in discussion and research. NGAL plays an important role in the occurrence and development of various tumors, and is a novel oncogene. It was found that the expression of NGAL in cervical cancer was markedly elevated, associated with its occurrence and metastasis. NGAL is expressed in cervical cancer relative to paracancerous tissues, inhibition of NGAL gene expression in thyroid cancer can reduce migration and invasion of cancer cells, and overexpression can promote migration and invasion of cancer cells, so that proliferation of cervical cancer cells is remarkably reduced and apoptosis is increased after NGAL expression is inhibited.
Based on the importance of NGAL as a label, development of a detection kit for detecting NGAL is an important direction of research. In physiological situations, NGAL is expressed very poorly in some organs in the body, including kidneys, cancerous tissues. Therefore, the sensitivity of detection is required to be high. In the prior art, PCR primers are designed and synthesized according to the NGAL gene sequence, a specific fragment amplified by PCR is cloned into a T vector, and a recombinant plasmid is used as a standard substance after screening, identification and quantification. FQ-PCR primers are designed in the NGAL amplified fragments, and PCR reaction conditions such as PCR primer concentration, annealing temperature, SYBR greenI dye concentration and the like are optimized in an orthogonal design mode, so that a detection method of NGAL is established. However, the method in the prior art is mainly a PCR method, has high requirements on instruments and equipment, is inconvenient to detect, and needs a new method which can be conveniently detected without instruments.
Disclosure of Invention
The present invention addresses the shortcomings of the prior art by providing an improved method.
In one aspect, specific monoclonal antibodies are provided against NGAL.
Specifically, the monoclonal antibody is N-5H16, and the sequence of the light chain variable region is SEQ ID NO:1 and the heavy chain variable region sequence SEQ ID NO:2.
furthermore, the monoclonal antibodies of the invention may undergo one or two or more conservative amino acid mutations in the light chain variable region and the heavy chain variable region, and the mutated antibodies still retain the activity of the corresponding antibodies.
Further, the monoclonal antibody of the present invention can be suitably used in capturing a target protein. For example, after biotinylation and reaction with exosomes, the monoclonal antibodies of the invention can be isolated by using streptavidin solid phase magnetic beads.
In more detail, the monoclonal antibody or antibody fragment thereof of the present invention is first biotinylated according to a known method. Next, the protein to be detected was mixed with biotinylated antibody and reacted at 4℃overnight. Then, streptavidin solid-phase magnetic beads were added, and after further reaction at 4℃for 2 hours, separation was performed using a magnet, whereby the target protein bound to the biotinylated antibody was recovered.
Specifically, an organism sample derived from a subject is contacted with the monoclonal antibody or antibody fragment thereof of the present invention, and a target protein is isolated according to the aforementioned protein capture isolation method.
In the present specification, the body sample is not particularly limited as long as it is a body sample selected from the group consisting of blood, serum, and plasma. (immunoassay) immunoassay was performed using a kit of monoclonal antibodies of the present invention. Examples of the immunoassay method include: enzyme Immunoassay (EIA), enzyme immunoassay (ELISA), fluorescent Immunoassay (FIA), radioimmunoassay (RIA), luminescent immunoassay, immunoblotting, western blotting, and the like, are preferable from the viewpoint of being able to detect antibodies simply and with good sensitivity.
ELISA methods include: in general, the competition method, the sandwich method, and the like, the monoclonal antibody or the antibody fragment thereof of the present invention can be used as a solid-phase antibody in the sandwich method, or as both a solid-phase antibody and a labeled antibody, and thus the sandwich method is preferable. Next, one mode of a sandwich ELISA method is shown. First, the monoclonal antibody or an antibody fragment thereof of the present invention is immobilized and then contacted with a test sample containing exosomes to form a complex. Then, a labeled antibody obtained by modifying the monoclonal antibody or the antibody fragment thereof, the disease-specific protein antibody or the antibody fragment thereof of the present invention in the kit of the present invention is added thereto to form a further complex, and the label is detected, whereby the signal amount from the exosomes contained in the sample and the disease-specific protein amount contained in the sample can be measured, respectively. Accordingly, the present invention also provides a method of determining the presence of a protein, the method comprising contacting an organism sample derived from a subject with a monoclonal antibody contained in a kit of monoclonal antibodies of the invention to form the complex.
In the case of immobilizing the monoclonal antibody or the antibody fragment thereof of the present invention, the monoclonal antibody may be immobilized directly or may be immobilized via a known medium, for example, streptavidin.
The monoclonal antibodies or kits of the invention can be used to detect kidney disease or cancer.
Specific cancers are "cancers" and "tumors" as used herein mean or describe physiological conditions in mammals that are typically characterized by uncontrolled cell growth. Examples of cancers or tumors include, but are not limited to, carcinoma, lymphoma, blastoma (including medulloblastoma and retinoblastoma), sarcoma (including liposarcoma and synovial cell sarcoma), neuroendocrine tumor (including benign tumor, gastrinoma, and island cell carcinoma), mesothelioma, schwannoma (including auditory neuroma), meningioma, adenoma, melanoma, and leukemia or lymphoid malignancy. More specific examples of such cancers include squamous cell carcinoma (e.g., epithelial squamous cell carcinoma), lung cancer including small-cell lung cancer, non-small-cell lung cancer, lung adenoma and squamous lung cancer, peritoneal cancer, hepatocellular carcinoma, gastric (gastric) or stomach (stomach) cancer including gastrointestinal cancer, pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, hepatoma, breast cancer, colon cancer, rectal cancer, colorectal cancer, endometrial or uterine cancer, salivary gland cancer, kidney (kidney) or renal (renal) cancer, prostate cancer, vaginal cancer, thyroid cancer, liver cancer, anal cancer, penile cancer, testicular cancer, esophageal cancer, cholangiocarcinoma, and head and neck cancer. Preferably, the cancer is a solid tumor. The term "solid tumor" as used herein refers to a tumor selected from the group consisting of gastrointestinal cancer, pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, hepatoma, breast cancer, colon cancer, rectal cancer, colorectal cancer, endometrial or uterine cancer, salivary gland cancer, renal (kidney) or renal (renal) cancer, prostate cancer, vaginal cancer, thyroid cancer, liver cancer, anal cancer, penile cancer, testicular cancer, esophageal cancer, cholangiocarcinoma, and head and neck cancer.
Furthermore, the invention also provides a kit for detection, which contains the monoclonal antibody.
Further, the invention provides a detection test strip.
The test strip is an immunochromatography test strip and consists of a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad. The sample pad and the conjugate pad were made of glass fibers, and the absorbent pad was made of water-absorbent filter paper. Drawing a line on a nitrocellulose membrane by using a goat anti-mouse polyclonal antibody as a quality control zone; the monoclonal antibody was streaked on nitrocellulose membrane as a detection zone, the parameter was streaked at 0.1. Mu.L/mm, and the distance between the control zone and the detection zone was about 7mm. After streaking, nitrocellulose membranes were incubated in an incubator at 37 ℃. And superposing the absorption pad, the sample pad, the bonding pad and the nitrocellulose membrane, assembling into a complete chromatographic strip, cutting the chromatographic strip, and preserving the chromatographic strip in a dry and light-proof manner with the width of 4 mm/strip.
Advantageous effects
Compared with the prior art, the invention has the following advantages:
the invention provides a specific monoclonal antibody aiming at NGAL protein, and the antibody has better specificity and binding activity. The antibody is used for detecting kidney injury patients, and has the characteristics of strong specificity, high sensitivity, short detection time (15-20 minutes) and large detection sample range; the detection method does not need any special instrument or equipment, and has the characteristic of low detection cost; the detection kit is simple and convenient to operate, and does not need to be operated by professionals; the kit disclosed by the invention is convenient to store and has a good application prospect.
Drawings
FIG. 1 identification results of monoclonal antibody recognition epitopes
Detailed Description
The invention may be understood more readily by reference to the following detailed description of some embodiments of the invention and the examples included therein. Before the present invention is further described, it is to be understood that this invention is not limited to particular embodiments described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims.
EXAMPLE 1 preparation of NGAL monoclonal antibody
Immunization of BALB/c mice: the neutrophil gelatinase-associated lipocalin (NGAL) recombinant protein (goods number: kl-B388Ra01, KALANG) is respectively added with Freund's complete adjuvant and Freund's incomplete adjuvant for emulsification to prepare Freund's complete adjuvant immunogen and Freund's incomplete adjuvant immunogen, wherein the volume ratio of the NGAL recombinant protein to Freund's complete adjuvant and Freund's incomplete adjuvant is 1:1, a step of; 3 female BALB/c mice of 8 weeks old were immunized with Freund's complete adjuvant immunogen by subcutaneous multipoint injection at the back, 100. Mu.l/mouse; BALB/c mice were boosted with incomplete freund's adjuvant immunogen in the same manner and dose 14 days and 28 days after the first immunization, respectively; immunization 14d, tail blood sampling, mouse serum titer measurement, and cell fusion of the mouse No. 1 with the highest titer; 5 days before cell fusion, BALB/c mice were hyperimmunized with adjuvant-free NGAL protein at a dose of 50 μg/mouse by intraperitoneal injection.
The BALB/c mouse No. 1 after 5d over-immunization was primed to kill the mice, and the mice were sacrificed for fusion by conventional methods. Transferring the fused cells into a semisolid culture medium for culture, culturing the grown monoclonal antibodies in a 96-well culture plate, and primarily screening by adopting an ELISA method to obtain 10 strains of monoclonal antibodies with strongest positive reaction, screening 6 strains of positive hybridomas, and finally obtaining 2 strains of hybridoma cell strains capable of stably secreting antibodies through 3 times of subcloning and propagation, wherein the hybridoma cell strains are named N-3A4 and N-5H16 respectively.
Determination of the ascites Titer of mab 1 week before preparation, paraffin was injected intraperitoneally into mice at 500. Mu.L/mouse, and after the enlarged culture of hybridomas, 10% of the total amount of the antibody was used per mouse 6 The abdominal cavity of each cell number is injected with 200 mu L of hybridoma cell suspension, the abdominal circumference of the mice is obviously increased after 6d, ascites is collected, and the titer of the ascites is measured by an indirect ELISA method. The results showed that the titer of ascites due to the N-3A4 monoclonal antibody was 1.28X10 7 The titer of ascites by the N-5H16 monoclonal antibody is 5.12X10 7 . And purifying the two antibodies by using a Protein G affinity column, wherein the purity of the two purified antibodies reaches more than 99% after SDS-PAGE Protein electrophoresis analysis.
EXAMPLE 2N-5H16 monoclonal antibody characterization
(1) Monomeric Ig subclass identification: the identification of the mab subclass was performed according to the instructions of the mouse mab subtype identification kit from Sigma. The results show that according to the antibody detection kit monoclonal antibody subclass detection method, the subtype of the N-5H16 monoclonal antibody is IgG1.
(2) Determination of monoclonal antibody affinity: and (3) coating an enzyme-labeled plate with NGAL recombinant protein at a concentration of 1 mug/mL by using an indirect ELISA method, adding purified N-5H16 monoclonal antibody diluted by a double ratio for incubation after sealing, taking goat anti-mouse IgG marked by HRP as a secondary antibody, and reading an OD450nm absorbance value by using an enzyme-labeled instrument. The OD450nm readings of several dilutions in succession are regarded as 100% binding of antigen-antibody when no longer increased, the ordinate is the absorbance at OD450nm, and the ordinate is the scatter plot, and the 50% binding of antigen-antibody when half of the maximum value of the readings is used to generate a logarithmic trend line and formula. Half of the OD450nm maximum was substituted into the formula, and the concentration of the antibody at this time was determined as affinity dissociation constant (Kd). The results are shown in Table 1 below.
Table 1 affinity of N-5H16 mab
Antibody name | Dissociation constant (Kd, M) |
N-5H16 monoclonal antibody | 3.86×10 -10 |
(3) N-5H16 monoclonal antibody specificity identification is carried out by respectively carrying out identification on N-5H16 monoclonal antibody specificity by using BSA, PD-1, NGAL and escherichia coli lysate, carrying out Western Blot after different proteins are diluted by a certain multiple, respectively using diluted monoclonal antibodies (1:5000) as primary antibodies, and detecting cross reaction of the obtained N-5H16 monoclonal antibodies on common different proteins. The results show that the N-5H16 monoclonal antibody provided by the invention only binds with NGAL protein but not other proteins, and has better specificity.
(4) And (3) through an antibody sequence identification kit, and through sequencing, the light and heavy chain variable region sequences of the N-5H16 monoclonal antibody are respectively shown in SEQ ID Nos: 1-2.
Example 3 identification of N-5H16 monoclonal antibody recognition epitope
A total of 4 NGAL truncations were designed based on the NGAL sequence, designated A (1-594 bp), B (1-477 bp), C (1-357 bp) and D (1-237 bp), respectively. The primers (shown in Table 2) were designed according to the truncations, and human DNA was used as templates to amplify the truncations DNA fragments, and NcoI site and EcoRI site were introduced at the 5 'and 3' ends, respectively.
TABLE 2 amplification primers
Western blot analysis is carried out by taking NGAL total protein and a1-A4 as antigens and N-3A4 monoclonal antibody as primary antibodies. It was found by analysis that N-5H16 mab was able to bind to whole protein as well as to the a1, a2 and a3 short peptides (FIG. 1), but not to a4, indicating that the antibody binding site of the invention is between aa 79-159.
Example 4 preparation of colloidal gold immunochromatographic test strip
Preparing colloidal gold particles: and preparing 25nm colloidal gold by adopting a trisodium citrate reduction method. The specific operation method is as follows: 100mL of double distilled water is taken, heated to boiling, 1mL of 1% chloroauric acid is added, and 1.5mL of freshly prepared trisodium citrate aqueous solution with a volume fraction of 1% is accurately added under stirring. At this time, it was observed that the pale yellow aqueous chloroauric acid solution became grey soon after the addition of trisodium citrate, and subsequently turned black, and then gradually stabilized to a reddish wine color, and after 20 minutes, the heating was stopped. Cooling to room temperature and storing in dark at 4 ℃.
The optimal pH of the colloidal gold solution was determined. Taking 8 parts of 1mL of colloidal gold solution, and respectively using 0.2mol/LK 2 CO 3 Adjusting the pH value to 5.0, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0; 100 mu L of N-5H16 monoclonal antibody with the mass fraction of 0.2 mu g/mL is added respectively, evenly mixed, kept stand for 10min, 100 mu L of NaCl solution with the mass fraction of 10% is added, kept stand for 0.5H, and then the color of colloidal gold is observed. Lowest p of unchanged color of colloidal goldThe H value is the optimal pH value of the colloidal gold solution. The result showed that the lowest pH value at which the color of the colloidal gold was unchanged was 7.5.
Determining the minimum protein stabilizing amount: the minimum amount of protein required to stabilize a certain amount of colloidal gold should be first determined before labeling. Adjusting the optimal pH value of the colloidal gold solution to 7.5, removing large aggregates of the antigen by high-speed centrifugation, diluting to 0.2mg/ml by using a PB solution of 5mmol/LPH8.0, gradually diluting the N-5H16 monoclonal antibody to be marked, sequentially adding the equal volumes into a series of test tubes filled with 1ml of colloidal gold, and uniformly mixing; after 5min, 0.1ml of 10% sodium chloride solution was added to each of the above-mentioned tubes, and the mixture was mixed and allowed to stand at room temperature, and the mixture was measured by a spectrophotometer OD 580. The test tubes without protein and with insufficient protein added to stabilize the colloidal gold show a coagulation phenomenon from red to blue, while the test tubes with protein added to reach or exceed the minimum stabilizing amount keep the red color of the colloidal gold unchanged. The amount of protein in the test tube with the lowest protein content, which keeps the colloidal gold red unchanged, is the necessary protein amount for stabilizing 1ml of colloidal gold, namely the lowest stabilizing amount. The actual optimum amount of protein required for stabilizing 1ml of colloidal gold is obtained by adding 20% of the colloidal gold. As a result, the N-5H16 antibody of the present invention was suitably used as the actual minimum protein labeling amount by selecting the amount of the N-5H16 monoclonal antibody added to 10. Mu.g/mL.
The colloidal gold was adjusted to an optimal pH of 7.5 and a minimum protein stabilizing amount of N-5H16 monoclonal antibody was added. After standing for 5min, adding excessive BSA for blocking, centrifuging, re-suspending with 0.01mol/LTris solution, centrifuging at low speed to remove aggregates, and obtaining supernatant as the colloidal gold labeled N-5H16 antibody.
The immunochromatography test strip consists of a sample pad, a binding pad, a nitrocellulose membrane and an absorption pad. The sample pad and the conjugate pad were made of glass fibers, and the absorbent pad was made of water-absorbent filter paper. Marking a goat anti-mouse polyclonal antibody with the concentration of 1mg/mL on a nitrocellulose membrane to be used as a quality control zone; the N-5H16 monoclonal antibody with the concentration of 1mg/mL is streaked on the nitrocellulose membrane to be used as a detection zone, the parameter is streaked at 0.1 mu L/mm, and the distance between the finger control zone and the detection zone is about 7mm. After streaking, the nitrocellulose membrane was incubated in an incubator at 37℃for 2h. And superposing the absorption pad, the sample pad, the bonding pad and the nitrocellulose membrane, assembling into a complete chromatographic strip, cutting the chromatographic strip, and preserving the chromatographic strip in a dry and light-proof manner with the width of 4 mm/strip.
And (5) detecting and judging results. And after 100 mu L of manual sample is added on a sample pad of the prepared chromatographic strip for 15min, the detection zone and the finger control zone are positive, only the finger control zone is negative, and the detection zone and the finger control zone are not developed, so that the reagent is invalid and a new test strip is needed to be replaced for retesting.
The test example collects 200 urine samples, wherein 85 positive samples (kidney injury patients) and 115 negative samples are detected. The sample is detected by a QuicKey-human neutrophil gelatinase related lipocalin (NGAL) enzyme-linked immunosorbent assay kit (product number: E-TSEL-H0003, elabscience), and the positive results are positive and the negative results are negative. The test paper strip provided by the embodiment of the invention is used for respectively detecting the collected 200 urine samples, and the detection results are summarized. The consistency of the test strip of the embodiment of the invention and the control test strip is evaluated by analyzing the yin-yang consistency ratio and about sign index of 135 parts of data by using a four-grid table and performing Kappa test. The results are shown in Table 3.
TABLE 3 results of clinical Performance verification
From table 3, the calculation results are as follows:
positive sample coincidence rate (true positive rate) =85/85×100% =100%
Negative sample coincidence rate (true negative rate) =115/115×100% =100%
Total compliance = 200/200 x 100% = 100%
About dengue index=85/85+115/115-1=1
kappa value=1, indicating that the test strip of example 1 of the present invention is consistent well with the control strip.
Detection result coincidence rate= (total number of samples-difference number of samples)/total number of samples×100% = (200-0)/200×100% = 100%
From the above data, it can be seen that the clinical verification is performed by using the neutrophil gelatinase-associated lipocalin (NGAL) test strip (colloidal gold method) of the embodiment of the invention, and 200 samples (85 positive samples and 115 negative samples) of the urine are detected by using the commercial neutrophil gelatinase-associated lipocalin test strip (latex enhanced immunonephelometry) as a control, and the detection results are sorted and analyzed, so that the clinical performance of the kit is evaluated, wherein the positive coincidence rate is 100%, the negative coincidence rate is 100%, the total coincidence rate is 100%, and the about log index is 1. The Kappa value is 1 (Kappa test, P < 0.05), the coincidence rate of the detection result is 100%, and the detection test strip in the embodiment 1 of the invention has good consistency with the control test strip. The test result shows that the test strip provided by the embodiment of the invention has the advantages of similar detection performance as the control test strip, good stability and accurate and reliable result. The test strip is simple and convenient to operate, has lower cost than the imported test strip, and has good market application value.
While the present invention has been described in connection with the present embodiments, it is not to be considered as limiting the scope of the invention, which is defined by the appended claims. In addition, various changes or modifications may be made by those skilled in the art within the scope of the present invention as defined in the appended claims, and such changes or modifications are also within the scope of the present invention.
Claims (5)
1. A monoclonal antibody specific for NGAL neutrophil gelatinase-associated lipocalin NGAL, characterized in that said monoclonal antibody is N-5H16, the sequence of the light chain variable region of which is SEQ ID NO:1 and the heavy chain variable region sequence SEQ ID NO:2.
2. use of the monoclonal antibody N-5H16 according to claim 1 for the preparation of a kit for detecting NGAL protein content in a sample.
3. The use according to claim 2, wherein the kit comprises a test strip.
4. The method of claim 3, wherein the test strip comprises 4 parts of a sample pad, a binding pad, a nitrocellulose membrane and an absorption pad.
5. The use according to claim 4, wherein the sample pad and the conjugate pad are glass fibers and the absorbent pad is a water-absorbent filter paper; drawing a line on a nitrocellulose membrane by using a goat anti-mouse polyclonal antibody as a quality control zone; monoclonal antibodies were streaked on nitrocellulose membranes as detection bands.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211126835.2A CN115991768B (en) | 2022-09-16 | 2022-09-16 | Neutrophil gelatinase-associated lipocalin (NGAL) detection kit |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211126835.2A CN115991768B (en) | 2022-09-16 | 2022-09-16 | Neutrophil gelatinase-associated lipocalin (NGAL) detection kit |
Publications (2)
Publication Number | Publication Date |
---|---|
CN115991768A true CN115991768A (en) | 2023-04-21 |
CN115991768B CN115991768B (en) | 2023-07-07 |
Family
ID=85994328
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202211126835.2A Active CN115991768B (en) | 2022-09-16 | 2022-09-16 | Neutrophil gelatinase-associated lipocalin (NGAL) detection kit |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN115991768B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117497170A (en) * | 2023-10-30 | 2024-02-02 | 南方医科大学南方医院 | Construction method and application of early warning model for converting acute kidney injury into chronic kidney disease |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103074303A (en) * | 2012-12-27 | 2013-05-01 | 中国人民解放军第三军医大学 | Hybridoma cell strain generating anti-human NGAL specific monoclonal antibody, monoclonal antibody generated by same and application |
CN203011928U (en) * | 2013-01-16 | 2013-06-19 | 武汉华美生物工程有限公司 | Test strip for detecting neutrophile granulocyte gelatinase associated lipocalin |
CN104181305A (en) * | 2013-05-27 | 2014-12-03 | 中国科学院上海生命科学研究院 | Anti-human neutrophil gelatinase-associated lipocalin antibodies and use thereof |
CN109553682A (en) * | 2018-12-29 | 2019-04-02 | 江苏众红生物工程创药研究院有限公司 | Anti-human neutrophil gelatinase-associated lipocalin antibody and its application in Test paper card |
CN109608542A (en) * | 2018-12-29 | 2019-04-12 | 江苏众红生物工程创药研究院有限公司 | Anti-human NGAL antibody and its application in Test paper card |
-
2022
- 2022-09-16 CN CN202211126835.2A patent/CN115991768B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103074303A (en) * | 2012-12-27 | 2013-05-01 | 中国人民解放军第三军医大学 | Hybridoma cell strain generating anti-human NGAL specific monoclonal antibody, monoclonal antibody generated by same and application |
CN203011928U (en) * | 2013-01-16 | 2013-06-19 | 武汉华美生物工程有限公司 | Test strip for detecting neutrophile granulocyte gelatinase associated lipocalin |
CN104181305A (en) * | 2013-05-27 | 2014-12-03 | 中国科学院上海生命科学研究院 | Anti-human neutrophil gelatinase-associated lipocalin antibodies and use thereof |
CN109553682A (en) * | 2018-12-29 | 2019-04-02 | 江苏众红生物工程创药研究院有限公司 | Anti-human neutrophil gelatinase-associated lipocalin antibody and its application in Test paper card |
CN109608542A (en) * | 2018-12-29 | 2019-04-12 | 江苏众红生物工程创药研究院有限公司 | Anti-human NGAL antibody and its application in Test paper card |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117497170A (en) * | 2023-10-30 | 2024-02-02 | 南方医科大学南方医院 | Construction method and application of early warning model for converting acute kidney injury into chronic kidney disease |
Also Published As
Publication number | Publication date |
---|---|
CN115991768B (en) | 2023-07-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7090983B1 (en) | Methods for detecting early cancer | |
WO2017107974A1 (en) | Detection test kit for serum psmd4 proteins and detection method and application thereof | |
US7205118B2 (en) | Nicotinamide N-methyltransferase as a marker for colorectal cancer | |
US9097714B2 (en) | Method for diagnosing malignant tumor | |
RU2769987C2 (en) | Antibody analysis | |
CN113248609B (en) | Antibody combination for regenerated islet-derived protein 1alpha and detection kit comprising same | |
US20060177880A1 (en) | Use of PRN3/ILEU as a marker for colorectal cancer | |
CN115991768B (en) | Neutrophil gelatinase-associated lipocalin (NGAL) detection kit | |
US20060188949A1 (en) | Use of protein PLST as a marker for colorectal cancer | |
US7579158B2 (en) | Cellular retinoic acid binding protein II as a marker for breast cancer | |
WO2011108628A1 (en) | Gastric cancer marker, and method for detecting gastric cancer | |
US20060121540A1 (en) | Use of protein MASP as a marker for colorectal cancer | |
CN111735946B (en) | Serum ALDH1B1 autoantibody quantitative detection kit and application thereof | |
CN107110848B (en) | Method for detecting arteriosclerosis and cancer using deoxyhypusine synthase gene as index | |
CN116655788B (en) | Neutrophil gelatinase-associated lipocalin (NGAL) urine detection kit | |
EP3129785B1 (en) | Diagnosis of cancer by detecting dimeric il-18 | |
CN111303289B (en) | Anti-human Tn-type glycosylated MUC1 antibody and application thereof | |
JP2014115186A (en) | Method for detecting stomach cancer, lung cancer, and/or esophagus cancer | |
US20100221742A1 (en) | Novel cancer associated antibodies and their use in cancer diagnosis | |
JP4292208B2 (en) | Use of protein SPEE as a marker for breast cancer | |
WO2021030450A1 (en) | Novel lox-1 antibody compositions, lox1 neutralization assay and methods of treatment using same | |
CN111793136A (en) | NMP22 antibody pair and application thereof | |
WO2021246153A1 (en) | Method and reagent for detecting pancreatic cancers | |
WO2013176070A1 (en) | Cancer detection method | |
WO2018034332A1 (en) | EphA2 N-TERMINUS FRAGMENT ANTIBODY |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |