CN115956625A - Production method and application of palm meal fermented feed - Google Patents

Production method and application of palm meal fermented feed Download PDF

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CN115956625A
CN115956625A CN202211663134.2A CN202211663134A CN115956625A CN 115956625 A CN115956625 A CN 115956625A CN 202211663134 A CN202211663134 A CN 202211663134A CN 115956625 A CN115956625 A CN 115956625A
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palm meal
fermentation
palm
fermented feed
meal
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谢建华
邹高溪
朱帅
蒋再慧
罗志威
吕江漫
龙敏
邹梓芳
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Tangrenshen Group Co ltd
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    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention provides a production method and application of a palm meal fermented feed, which comprises the following steps: preparing a palm meal enzymolysis liquid, wherein the palm meal enzymolysis liquid is used for carrying out enzymolysis on palm meal to form an enzymolysis liquid which can be used for culturing a microbial inoculum; preparing a palm meal fermentation activation solution, and performing activation culture on the composite zymophyte agent by using enzymolysis liquid; and a feed fermentation process, wherein the activated microbial inoculum and a fermentation medium are mixed and fermented to form the palm meal fermented feed. According to the invention, liquid and solid multi-stage deep enzymolysis fermentation is adopted, so that the anti-nutritional ingredients in the palm meal are effectively degraded, the digestion and utilization rate of animals on the palm meal is improved, meanwhile, rich small peptides, organic acids, bacteriostats, probiotics and the like are generated, and the intestinal health of the animals can be improved. The process improves the palatability and the nutritional value of the palm meal, and replaces the corn and soybean meal in the poultry feed in a certain proportion, thereby reducing the feed cost and improving the quality of egg products.

Description

Production method and application of palm meal fermented feed
Technical Field
The invention belongs to the field of bioengineering, and particularly relates to a production method and application of a palm meal fermented feed.
Background
With the development of animal husbandry, 40 to 50 percent of raw grains are directly used in the feed processing process every year in China, so that the problems of feed grain shortage and grain competition between people and livestock are increasingly prominent. The research on the effective utilization of the unconventional raw materials in the livestock and poultry breeding becomes a hot spot of the industry. The palm meal is a byproduct of palm kernel shelling and oil pressing, and is rich in resources and low in price. The palm meal has 14-21% of crude protein and 8-17% of lipid, can provide protein and energy for animals at the same time, and has very good application potential in livestock and poultry feeds. However, the content of the crude fiber of the palm meal is up to more than 15%, the content of non-starch polysaccharide is high, and especially the proportion of mannan is large, so that the palatability is influenced, the conversion and utilization of animals are hindered, and the application of the palm meal in livestock and poultry feed is greatly limited.
From the aspects of nutritional value and chemical components, the palm meal has extremely high fermentation plasticity, the high-content crude fiber and non-starch polysaccharide can be reduced through the fermentation process of non-starch polysaccharidase and microorganisms, the energy utilization rate is improved, the digestion energy and the metabolic energy are correspondingly improved, and particularly, the non-starch polysaccharide can be fermented to generate a large amount of functional oligosaccharide, so that the immune function of animals is improved. Penghai dragon and other reports report that 4% -7% of daily ration of fermented palm meal is added to feed growing pigs, so that the feed intake and growth development of the pigs can be improved; 4% of fermented palm meal is added into the daily ration, the daily egg weight, the Ha's unit and the egg white height of the laying hens are all improved, and the color grade of egg yolks, the egg laying performance of the laying hens and the egg quality are all improved to different degrees.
The palm meal biological treatment method mainly comprises a microbial fermentation method, a biological enzymolysis method and an enzyme-bacterium synergistic fermentation method. The microbial fermented palm meal can produce rich functional metabolites (lactic acid, volatile fatty acid, bacteriocin and the like) and a large amount of probiotics, but the main anti-nutritional factors (40-42% non-starch polysaccharide) in the palm meal are not fully degraded, a large amount of anti-nutritional factors still exist in the fermented palm meal finished product, the digestibility of energy and fiber is not fundamentally improved, and the feeding value is not effectively improved. The biological enzymolysis of the palm meal refers to that the macromolecular polysaccharide is cut into oligosaccharide and the like by adopting endo-hydrolytic enzymes such as beta-mannase, beta-xylanase and the like so as to eliminate the anti-nutritional effect and improve the digestibility of the palm meal. However, the cell wall structure of the palm meal is compact, the combination efficiency of the biological enzyme and the substrate is low without pretreatment, and the main anti-nutritional factors of the palm meal, namely mannan and the like, are difficult to completely eliminate. Meanwhile, compared with the fermented palm meal, the enzymolysis palm meal is lack of metabolites with immune functions, such as organic acids, bacteriocins and the like. In addition, at present, only endo-hydrolase is considered for enzymolysis, the endo-hydrolase can only hydrolyze and cut polysaccharides into oligosaccharides or polysaccharides, substrate feedback inhibition exists, the polysaccharides such as mannan cannot be completely hydrolyzed, and different exoglycosidases are required for complete hydrolysis, such as beta-mannase, alpha-galactosidase and the like.
Disclosure of Invention
The invention provides a production method and application of palm meal fermented feed, which are used for effectively utilizing palm meal to prepare the palm meal fermented feed rich in metabolites such as probiotics, organic acids, small peptides, bacteriocins and the like, so that the economic value of the palm meal in the livestock and poultry feed industry is improved.
The invention provides a production method of a palm meal fermented feed, which comprises the following steps:
preparing a palm meal enzymolysis liquid, wherein the palm meal enzymolysis liquid is used for carrying out enzymolysis on palm meal to form an enzymolysis liquid used for culturing a microbial inoculum;
preparing a palm meal fermentation activation solution, wherein the palm meal fermentation activation solution is used for performing activation culture on a composite zymophyte microbial inoculum by using enzymolysis liquid;
and a feed fermentation process, wherein the feed fermentation process is used for mixing and fermenting the activated microbial inoculum and a fermentation medium to form the palm meal fermented feed.
Further, the process for preparing the palm meal enzymolysis liquid comprises the following steps:
s101, removing impurities from palm meal, crushing, and heating for pretreatment;
s102, adding a complex enzyme for degradation into the processed palm meal, and carrying out enzymolysis for 1-3h to obtain palm meal enzymolysis liquid.
Further, the compound enzyme comprises: 5000-1000U/g of acid protease, 5000-120000U/g of neutral protease, 20000-40000U/g of beta-mannase, 1000-3000U/g of alpha-galactosidase, 50-100U/g of beta-mannosidase and 1000-2000U/g of xylanase.
Further, the process for preparing the palm meal fermentation activating solution comprises the following steps:
adding a composite fermentation microbial inoculum into the palm meal enzymatic hydrolysate, and culturing to obtain a palm meal fermentation activating solution;
the composite fermentation inoculum at least comprises two or more than two of lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis and saccharomyces cerevisiae.
Still further, the complex fermentation inoculant comprises: lactobacillus plantarum 2.0-5.0 × 10 8 cfu/g, pediococcus pentosaceus 1.0-3.0X 10 8 cfu/g, 0.5-1.0X 10 of Bacillus subtilis 8 cfu/g, saccharomyces cerevisiae 5-10 × 10 7 cfu/g。
Further, the feed fermentation process comprises:
s201, mixing the palm meal fermentation activating solution and a fermentation dry base;
s202, carrying out anaerobic solid fermentation in a fermentation container for 2-6d to obtain a palm meal fermented feed;
the fermentation dry base comprises: 10-30 parts of bran, 2-5 parts of brown sugar and 65-88 parts of palm meal.
The invention also discloses a palm meal fermented feed, which is prepared by applying the production method.
Furthermore, the small peptide content in the palm meal fermented feed is 5.0-30% of the protein content, and the viable count of probiotics is 2.0-20 x 10 8 CFU/g, total acid content of 25-45 g/kg, and reducing sugar content of 10.0-20.0%.
Furthermore, the content of small peptides in the palm meal fermented feed is 8.0-15.0% of the crude protein, and the number of viable probiotics is 2.0-10 x 10 8 CFU/g。
The third aspect of the invention also discloses a biological feed, wherein the addition amount of the palm meal fermented feed in the biological feed is 5-20%.
Compared with the prior art, the invention has the following advantages:
1. beta-mannosidase and alpha-galactosase exoglycosidase are newly added, mannan which is a main anti-nutritional factor in the palm meal is further degraded into partial glucose, mannose and lactose, the feedback inhibition of an enzymolysis reaction is removed, the enzymolysis efficiency is improved, the anti-nutritional effect of the palm meal is further removed, and the digestion and utilization efficiency of animals on the palm meal is improved;
2. the method integrates the liquid state deep fermentation process and the solid state fermentation process of the palm meal into one, wherein the micromolecule saccharides generated by enzymolysis can be used as main nutrient sources for activation and proliferation, so that the dependence on high-value raw materials such as glucose, cane sugar and the like is reduced, the feeding process in the process is simplified, and the production cost is reduced;
3. according to the invention, the liquid enzymolysis process, the liquid micro-aerobic fermentation process and the solid anaerobic fermentation multi-stage fermentation process are used for carrying out efficient deep fermentation on the palm meal, so that the content of mannan and macromolecules in the palm meal is effectively degraded, abundant functional small peptides, probiotics, organic acids, bacteriocins and other metabolic products are generated, the biological energy utilization rate and the immune function of the palm meal are improved, and the stability of the product is improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention, illustrate embodiments of the invention and together with the description serve to explain the invention and do not constitute a limitation of the invention.
FIG. 1 is a schematic flow diagram of a process for producing a palm meal fermented feed according to the present invention;
FIG. 2 shows the bacteriostatic effect of the palm meal fermented feed on clostridium perfringens, and an oxford cup detection bacteriostatic ring method is adopted in the detection method.
Wherein CK is 100 mul, the inhibition zone of chlortetracycline hydrochloride with the concentration of 150ppm to clostridium perfringens, A and B are respectively the inhibition zone of 100 mul of leachate of the palm meal fermented feed produced in the embodiment 1 and the embodiment 2 (the proportion of biological feed to water is 1).
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are all conventional methods unless otherwise specified.
In order to make those skilled in the art better understand the technical solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The first aspect of the embodiment of the invention provides a production process for deep fermentation of palm meal by cooperation of multi-stage enzyme bacteria, which comprises the following steps:
preparing a palm meal enzymolysis liquid, wherein the palm meal enzymolysis liquid is used for carrying out enzymolysis on palm meal to form an enzymolysis liquid used for culturing a microbial inoculum;
preparing a palm meal fermentation activation solution, wherein the palm meal fermentation activation solution is used for performing activation culture on a composite zymophyte microbial inoculum by using enzymolysis liquid;
and a feed fermentation process, wherein the feed fermentation process is used for mixing and fermenting the activated microbial inoculum and a fermentation medium to form the palm meal fermented feed.
According to the embodiment of the invention, the palm meal enzymolysis liquid preparation process, the palm meal fermentation activating liquid preparation process and the feed fermentation process are respectively carried out, and a person skilled in the art can carry out treatment based on the optimal conditions of each step so as to optimize the feed fermentation degree.
Optionally, the process for preparing the palm meal enzymatic hydrolysate comprises the following steps:
s101, removing impurities from palm meal, crushing, and heating for pretreatment;
s102, adding a complex enzyme for degradation into the processed palm meal, and carrying out enzymolysis for 1-3h to obtain palm meal enzymolysis liquid.
Wherein, the compound enzyme comprises: 5000-1000U/g of acid protease, 5000-120000U/g of neutral protease, 20000-40000U/g of beta-mannase, 1000-3000U/g of alpha-galactosidase, 50-100U/g of beta-mannosidase and 1000-2000U/g of xylanase.
The complex enzyme adopted by the embodiment of the invention can comprehensively degrade various components of the palm meal.
Optionally, the process for preparing the palm meal fermentation activation solution comprises the following steps:
adding a composite fermentation microbial inoculum into the palm meal enzymatic hydrolysate, and culturing to obtain a palm meal fermentation activating solution;
the composite fermentation bacteria agent at least comprises two or more than two of lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis and saccharomyces cerevisiae.
Wherein, the compound fermentation inoculant comprises: lactobacillus plantarum 2.0-5.0 × 10 8 cfu/g, pediococcus pentosaceus 1.0-3.0X 10 8 cfu/g, 0.5-1.0X 10 Bacillus subtilis 8 cfu/g, saccharomyces cerevisiae 5-10 × 10 7 cfu/g。
According to the embodiment of the invention, the palm meal can be comprehensively degraded by adopting the composite fermentation inoculant, and metabolic products such as abundant functional small peptides, probiotics, organic acids and bacteriocins are generated.
Optionally, the feed fermentation process comprises:
s201, mixing the palm meal fermentation activating solution and the fermentation dry base;
s202, carrying out anaerobic solid fermentation in a fermentation container for 2-6d to obtain a palm meal fermented feed;
the fermentation dry base comprises: 10-30 parts of bran, 2-5 parts of brown sugar and 65-88 parts of palm meal.
The second aspect of the embodiment of the invention also discloses a palm meal fermented feed which is prepared by applying the production method.
Optionally, the content of small peptides in the palm meal fermented feed is 5.0-30% of the protein contained in the palm meal fermented feed, and the number of viable probiotics is 2.0-20 x 10 8 CFU/g, total acid content of 25-45 g/kg, and reducing sugar content of 10.0-20.0%.
Optionally, the content of small peptides in the palm meal fermented feed is 8.0-15.0% of the crude protein, and the number of viable probiotics is 2.0-10 x 10 8 CFU/g。
The third aspect of the embodiment of the invention also discloses a biological feed, wherein the addition amount of the palm meal fermented feed in the biological feed is 5-20%.
In examples 1 to 3 of the present invention, the neutral protease and the β -xylanase were provided by Guangdong overflow Multi biosciences, inc., and the β -mannanase, the α -galactosidase and the β -mannosidase were provided by Dongguan Panasia Tai biosciences, inc. The lactobacillus plantarum is screened by the inventor, and the preservation number is ACCC10171; pediococcus pentosaceus accession number: CICC 22734; saccharomyces cerevisiae was purchased from Angel Yeast, inc. of Hubei; bacillus subtilis was purchased from Zhejiang Huijia Biotech GmbH.
Example 1
In the embodiment 1 of the invention, the traditional enzyme-bacterium synergistic solid-state fermentation is adopted to prepare the palm meal fermented feed (the traditional enzyme-bacterium synergistic fermentation process) and the preparation method is as follows (refer to patent CN 202011292693.8):
step 1: 1.0 kg of composite fermentation microbial inoculum (microbial inoculum composition: lactobacillus plantarum 5 multiplied by 10) 8 cfu/g, pentose tablet ball5X 10 fungus 8 cfu/g, bacillus subtilis 5X 10 7 cfu/g, saccharomyces cerevisiae 1X 10 7 cfu/g), 1.0 kg of synthase preparation (enzyme activity: 10000U/g of acid protease, 20000U/g of neutral protease, 30000U/g of beta-mannase, 20000U/g of xylanase and 5 kg of brown sugar are dissolved in 100 kg of warm water (30-37 ℃) and uniformly mixed to prepare the bacteria liquid water.
And 2, step: weighing 475 kg of palm meal and 140 kg of wheat bran, crushing the materials into particles of 2.0mm, adding 15 kg of brown sugar, and preparing a fermentation dry base; and then, uniformly mixing 630 kg of fermentation dry base, 100 kg of bacterial liquid water and 265 kg of tap water, filling the mixture into a packaging bag with a breather valve, sealing and stacking the packaging bag with 40 kg of each packaging bag in a fermentation room with the temperature of 30 +/-5 ℃ for fermentation for 5 days, and sampling every day during the fermentation process to detect the content of water, pH, crude protein, small peptide, total acid and viable count.
And (3) setting a control group, specifically palm meal without the fermentation inoculum, weighing 63 kg of fermentation dry base obtained in the step (2) and 36.5 kg of tap water, uniformly mixing, filling into a packaging bag, and sampling to detect various indexes in the same step (2).
Example 2
The preparation method of the palm meal fermented feed through multi-stage enzyme-bacterium synergistic fermentation in the embodiment 2 of the invention comprises the following specific steps:
step 1: weighing 125 kg of palm meal crushed into particles of 1.0mm, 10 kg of cane sugar and 365 kg of water, putting the materials into a 1.0 ton fermentation tank, uniformly stirring, heating to 85 ℃, keeping the temperature for 20min, adjusting the pH to 5.5 by using l.0mol/L phosphoric acid solution when the temperature is reduced to 50 ℃, adding 1 kg of complex enzyme preparation (enzyme activity comprises 5000U/g of acid protease, 10000U/g of neutral protease, 30000U/g of beta-mannase, 20000U/g of xylanase, 1000U/g of alpha-galactosidase and 50U/g of beta-mannosidase), uniformly stirring, keeping the rotation speed of 100rpm, stirring, and carrying out constant-temperature enzymolysis for 3.0h to obtain the palm meal enzymatic hydrolysate.
Step 2: when the temperature of the palm meal enzymolysis liquid obtained in the step a is reduced to 35 ℃, adding 1 kg of compound fermentation bacteria (the bacteria comprises lactobacillus plantarum 5 multiplied by 10) 8 cfu/g, pediococcus pentosaceus 5X 10 8 cfu/g, bacillus subtilis 5X 10 7 cfu/g, saccharomyces cerevisiae1×10 7 cfu/g) under the conditions of 0.01Mpa of tank pressure, 100rpm of rotating speed and 37 ℃ of temperature, carrying out enrichment culture for 12h to obtain the zymophyte agent activating solution.
And 3, step 3: fully and uniformly stirring 500 kg of fermentation dry base (350 kg of palm meal, 100 kg of wheat bran and 10 kg of brown sugar) and the fermentation inoculum activation solution obtained in the step 2 by using a stirring machine 1, packaging into packaging bags with breather valves, wherein each bag is 40 kg, and hermetically stacking in a fermentation room at 30 +/-5 ℃ for fermentation for 5d; during the fermentation process, the pH, the small peptide content, the total acid and the viable count are detected by sampling every day. The detection result is shown in fig. 2, the inhibition zone of example 1 (a in fig. 2) is significantly smaller than the inhibition zone of example 2 (B in fig. 2), which shows that the content of small peptides, total acid and total number of probiotics in example 2 of the present invention are significantly increased compared with the prior art (example 1), and the produced abundant bacteriostat has significant bacteriostasis on clostridium perfringens.
Indexes of various components of the feeds in the embodiment and the comparative example are shown in the table.
Indexes of various components of enzyme-bacterium synergistic fermentation feed
Figure BDA0004014761360000061
Example 3
In example 3 of the present invention, 2160 helan brown laying hens at the late stage of laying at 400 days old were selected, and randomly divided into 3 groups according to the principle of random distribution, each group had 5 repeats, and each repeat had 144 repeats. The control group was fed with corn soybean meal type basal diet, the test group 1 was a diet prepared by adding 10% of the fermented palm meal feed prepared in example 1 to substitute 2% of corn 3% of soybean meal 5% of bran in the original formula, and the test group 2 was a diet prepared by adding 10% of the fermented palm meal feed prepared in example 2 to substitute 2% of corn 3% of soybean meal 5% of bran in the original formula, based on the control group diet, and subjected to a feeding test for 21 days. The results of the tests are shown in Table 2 below:
TABLE 2 influence of fermented palm meal on layer production Performance
Figure BDA0004014761360000071
As can be seen from table 2, the fermented palm meal feed prepared in embodiment 2 of the present invention can significantly increase the laying rate of laying hens by 5.78% and increase the eggshell strength after replacing corn and soybean meal bran in the feed. In addition, as the palm meal fermented feed in the embodiment 2 of the invention contains abundant probiotics and metabolites, in an actual breeding test, the intestinal health of the intestinal laying hens can be improved, the dirty egg rate is obviously reduced, and the death number of the laying hens is obviously less than that of other groups (a control group and a test 1). In the actual culture process of farmers, after the farmers use the palm meal fermented feed, the culture cost is reduced (the cost is saved by the formula, and 50-60 yuan is saved by each ton of batch feed due to the adoption of the fermented palm meal feed), and the economic income is increased (the egg sale income brought by the improvement of the laying rate).
Finally, it should be noted that the above-mentioned embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that the modifications and equivalents of the specific embodiments of the present invention can be made by those skilled in the art after reading the present specification, but these modifications and variations do not depart from the scope of the claims of the present application.

Claims (10)

1. The production method of the palm meal fermented feed is characterized by comprising the following steps:
the method comprises the following steps of preparing a palm meal enzymatic hydrolysate, wherein the palm meal enzymatic hydrolysate is used for carrying out enzymatic hydrolysis on palm meal to form an enzymatic hydrolysate which can be used for culturing a microbial inoculum;
preparing a palm meal fermentation activation solution, wherein the palm meal fermentation activation solution is used for performing activation culture on a composite zymophyte microbial inoculum by using enzymolysis liquid;
and a feed fermentation process, wherein the feed fermentation process is used for mixing and fermenting the activated microbial inoculum and a fermentation medium to form the palm meal fermented feed.
2. The method for producing the palm meal fermented feed according to claim 1, wherein the process for preparing the palm meal enzymatic hydrolysate comprises the following steps:
s101, removing impurities from palm meal, crushing, and heating for pretreatment;
s102, adding a complex enzyme for degradation into the processed palm meal, and carrying out enzymolysis for 1-3h to obtain palm meal enzymolysis liquid.
3. The production method of the palm meal fermented feed according to claim 2, wherein the complex enzyme comprises: 5000-1000U/g of acid protease, 5000-120000U/g of neutral protease, 20000-40000U/g of beta-mannase, 1000-3000U/g of alpha-galactosidase, 50-100U/g of beta-mannosidase and 1000-2000U/g of xylanase.
4. The method for producing the palm meal fermented feed according to claim 1, wherein the process for preparing the palm meal fermentation activating solution comprises the following steps:
adding a composite fermentation microbial inoculum into the palm meal enzymatic hydrolysate, and culturing to obtain a palm meal fermentation activating solution;
the composite fermentation bacteria agent at least comprises two or more than two of lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis and saccharomyces cerevisiae.
5. The production method of the palm meal fermented feed according to claim 4, wherein the composite fermentation inoculant comprises: lactobacillus plantarum 2.0-5.0 × 10 8 cfu/g, pediococcus pentosaceus 1.0-3.0X 10 8 cfu/g, 0.5-1.0X 10 of Bacillus subtilis 8 cfu/g, saccharomyces cerevisiae 5-10 × 10 7 cfu/g。
6. The method for producing the palm meal fermented feed according to claim 1, wherein the feed fermentation process comprises the following steps:
s201, mixing the palm meal fermentation activating solution and a fermentation dry base;
s202, carrying out anaerobic solid fermentation in a fermentation container for 2-6d to obtain a palm meal fermented feed;
the fermentation dry base comprises: 10-30 parts of bran, 2-5 parts of brown sugar and 65-88 parts of palm meal.
7. A palm meal fermented feed, which is characterized by being prepared by applying the production method of any one of claims 1-6.
8. The palm meal fermented feed according to claim 7, wherein the content of small peptides in the palm meal fermented feed is 5.0-30% of the protein contained in the palm meal fermented feed, and the number of viable probiotics is 2.0-20 x 10 8 CFU/g, total acid content of 25-45 g/kg, and reducing sugar content of 10.0-20.0%.
9. The palm meal fermented feed according to claim 8, wherein the content of small peptides in the palm meal fermented feed is 8.0-15.0% of the content of crude proteins, and the number of viable probiotics is 2.0-10 x 10 8 CFU/g。
10. A biological feed, characterized in that the palm meal fermented feed according to any one of claims 7 to 9 is added in an amount of 5% to 20%.
CN202211663134.2A 2022-12-23 2022-12-23 Production method and application of palm meal fermented feed Pending CN115956625A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116970513A (en) * 2023-05-12 2023-10-31 山东省农业科学院 Lactobacillus plantarum SQ6 and application thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116970513A (en) * 2023-05-12 2023-10-31 山东省农业科学院 Lactobacillus plantarum SQ6 and application thereof
CN116970513B (en) * 2023-05-12 2024-04-30 山东省农业科学院 Lactobacillus plantarum SQ6 and application thereof

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