CN1159437C - Ahylysantinfarctase thrombase and its production process - Google Patents
Ahylysantinfarctase thrombase and its production process Download PDFInfo
- Publication number
- CN1159437C CN1159437C CNB011155671A CN01115567A CN1159437C CN 1159437 C CN1159437 C CN 1159437C CN B011155671 A CNB011155671 A CN B011155671A CN 01115567 A CN01115567 A CN 01115567A CN 1159437 C CN1159437 C CN 1159437C
- Authority
- CN
- China
- Prior art keywords
- reptilase
- ahylysantinfarctase
- thrombase
- subunit
- thrombin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000190 Thrombin Proteins 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title abstract description 7
- 108010027612 Batroxobin Proteins 0.000 claims abstract description 53
- 108090000790 Enzymes Proteins 0.000 claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims abstract description 15
- 150000001413 amino acids Chemical class 0.000 claims abstract description 10
- 230000002439 hemostatic effect Effects 0.000 claims abstract description 8
- 238000004440 column chromatography Methods 0.000 claims abstract description 7
- 238000001742 protein purification Methods 0.000 claims abstract description 7
- 238000013016 damping Methods 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 18
- 230000011218 segmentation Effects 0.000 claims description 9
- 235000001014 amino acid Nutrition 0.000 claims description 8
- 238000010612 desalination reaction Methods 0.000 claims description 8
- 238000010828 elution Methods 0.000 claims description 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000000546 pharmaceutical excipient Substances 0.000 claims 1
- 238000007670 refining Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 16
- 239000003998 snake venom Substances 0.000 abstract description 14
- 229940088598 enzyme Drugs 0.000 abstract description 13
- 238000000746 purification Methods 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 7
- 241001505404 Deinagkistrodon acutus Species 0.000 abstract description 5
- 238000000926 separation method Methods 0.000 abstract description 5
- 229960004072 thrombin Drugs 0.000 abstract description 5
- 238000011160 research Methods 0.000 abstract description 4
- 238000005259 measurement Methods 0.000 abstract description 2
- 150000001450 anions Chemical class 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 239000011780 sodium chloride Substances 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 239000008176 lyophilized powder Substances 0.000 description 7
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 241001474977 Palla Species 0.000 description 5
- 229920005654 Sephadex Polymers 0.000 description 5
- 239000012507 Sephadex™ Substances 0.000 description 5
- 241000271897 Viperidae Species 0.000 description 5
- 230000023555 blood coagulation Effects 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 101000772006 Bombus ignitus Venom serine protease Bi-VSP Proteins 0.000 description 3
- 241001449342 Chlorocrambe hastata Species 0.000 description 3
- 239000012614 Q-Sepharose Substances 0.000 description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 238000005515 capillary zone electrophoresis Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 241000271039 Agkistrodon Species 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 241000270295 Serpentes Species 0.000 description 2
- 238000005571 anion exchange chromatography Methods 0.000 description 2
- 229940030225 antihemorrhagics Drugs 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 239000002874 hemostatic agent Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 231100000611 venom Toxicity 0.000 description 2
- 241000271517 Bothrops jararaca Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 229960002210 batroxobin Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 230000000025 haemostatic effect Effects 0.000 description 1
- 208000031169 hemorrhagic disease Diseases 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- CIJQGPVMMRXSQW-UHFFFAOYSA-M sodium;2-aminoacetic acid;hydroxide Chemical compound O.[Na+].NCC([O-])=O CIJQGPVMMRXSQW-UHFFFAOYSA-M 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011155671A CN1159437C (en) | 2001-04-29 | 2001-04-29 | Ahylysantinfarctase thrombase and its production process |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011155671A CN1159437C (en) | 2001-04-29 | 2001-04-29 | Ahylysantinfarctase thrombase and its production process |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1332241A CN1332241A (en) | 2002-01-23 |
CN1159437C true CN1159437C (en) | 2004-07-28 |
Family
ID=4662063
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB011155671A Expired - Lifetime CN1159437C (en) | 2001-04-29 | 2001-04-29 | Ahylysantinfarctase thrombase and its production process |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1159437C (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1218747C (en) * | 2003-08-14 | 2005-09-14 | 吴忠 | Medicinal use of agkistrodon acutus thrombase for treating hemorrhagic disease |
CN101684151B (en) * | 2008-09-26 | 2012-06-20 | 江苏正大天晴药业股份有限公司 | Protein matter with coagulation activity |
CN101358184A (en) * | 2008-09-27 | 2009-02-04 | 康辰医药股份有限公司 | Hemocoagulase agkistrodon |
CN110791491A (en) * | 2019-12-11 | 2020-02-14 | 昆明龙津药业股份有限公司 | Method for extracting defibrase from snake venom |
-
2001
- 2001-04-29 CN CNB011155671A patent/CN1159437C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CN1332241A (en) | 2002-01-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C06 | Publication | ||
PB01 | Publication | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: SHENYANG SHOUZHENG BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: KUNMING INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES Effective date: 20051111 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20051111 Address after: 11, C, International Building, 219 youth Avenue, No. 110016, Youth Street, Shenhe, Shenyang Patentee after: Shouzheng Biological Technology Co., Ltd., Shenyang Address before: 650223 No. 32 teaching road, Kunming, Yunnan Patentee before: Kuiming Animal Institute of Chinese Academy of Sciences |
|
ASS | Succession or assignment of patent right |
Owner name: LIAONING NUOKANG BIO-PHARMACEUTICALS CO., LTD. Free format text: FORMER OWNER: SHENYANG SHOUZHENG BIOTECHNOLOGY CO., LTD. Effective date: 20130917 |
|
C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 110000 SHENYANG, LIAONING PROVINCE TO: 110016 SHENYANG, LIAONING PROVINCE |
|
TR01 | Transfer of patent right |
Effective date of registration: 20130917 Address after: 110016 Liaoning Province, Shenyang Hunnan New District Nanping Road No. 18 Patentee after: Liaoning Nuokang Bio-pharmaceutical Co., Ltd. Address before: 110000, C, 11 floor, Huaxin International Building, No. 219, youth Avenue, Shenhe District, Liaoning, Shenyang Patentee before: Shouzheng Biological Technology Co., Ltd., Shenyang |
|
DD01 | Delivery of document by public notice |
Addressee: Wang Hongying Document name: Notification of Passing Examination on Formalities |
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C56 | Change in the name or address of the patentee | ||
CP01 | Change in the name or title of a patent holder |
Address after: 110016 Liaoning Province, Shenyang Hunnan New District Nanping Road No. 18 Patentee after: Liaoning Yuanda Nuokang Bio-Pharmaceuticals Co., Ltd. Address before: 110016 Liaoning Province, Shenyang Hunnan New District Nanping Road No. 18 Patentee before: Liaoning Nuokang Bio-pharmaceutical Co., Ltd. |
|
CP02 | Change in the address of a patent holder |
Address after: 110016 Liaoning province Shenyang Hunnan Nanping Road No. 18-1 Patentee after: Liaoning Yuanda Nuokang Bio-Pharmaceuticals Co., Ltd. Address before: 110016 Liaoning Province, Shenyang Hunnan New District Nanping Road No. 18 Patentee before: Liaoning Yuanda Nuokang Bio-Pharmaceuticals Co., Ltd. |
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CP02 | Change in the address of a patent holder | ||
CX01 | Expiry of patent term | ||
CX01 | Expiry of patent term |
Granted publication date: 20040728 |