CN115895844A - Cell poison gas exposure contamination experimental equipment - Google Patents

Abstract

The invention discloses cytotoxic gas exposure contamination experiment equipment, which relates to the technical field of contamination experiments and comprises a closed box and a liquid collecting assembly, wherein a culture dish is arranged in the closed box, the liquid collecting assembly is connected to the bottom of the culture dish, the liquid collecting assembly comprises a vertical pipe, an electric push rod, a liquid pushing push plate, a recovery pipe, a first pump body and a liquid collecting device, the left side of the bottom of the vertical pipe is connected with the recovery pipe, and a cell template is arranged in the culture dish. This cell poison gas exposes experimental facilities that infects, inside the injection principle that utilizes the liquid pushing push pedal to make the ascending motion injects the culture dish with quantitative cell culture liquid, this sets up and prevents that inside the pipeline has remaining cell culture liquid when making the culture dish inside have quantitative cell culture liquid, utilize the liquid pushing push pedal to make the descending motion in order to retrieve the cell culture liquid that has contacted with radon gas after the experiment again, and utilize the piece that generates heat to carry out high temperature disinfection to pipeline and culture dish inside along with the lift of liquid pushing pedal.

Description

Cell poison gas exposure contamination experimental equipment

Technical Field

The invention relates to the technical field of contamination experiments, in particular to cell toxic gas exposure contamination experimental equipment.

Background

Radon gas is an inert gas, widely exists in human life, not only can pollute the indoor environment, but also can stimulate the functions of a human body by various rays emitted by the radon gas, so that the health of the human body is influenced, radon is radioactive gas, alpha particles generated by decay of radon can cause radiation damage in the respiratory system of the human body after the radon enters the human body to initiate lung cancer, the radon gas is inhaled for a long time, the radon can be combined with the nervous system and fat tissues to cause damage to blood cells and induce cancer, and therefore, the harm of the radon gas to the cells can be known by using a cell exposure experiment method, and therefore, cell exposure contamination experiment equipment needs to be used.

The existing cell toxic gas exposure contamination experiment equipment is characterized in that a cell template is placed in a culture dish and cell culture solution with a corresponding proportion is prepared, and then radon gas is uniformly distributed on the surface of the cell template to carry out a radon gas contamination experiment.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a cell toxic gas exposure experimental device, which solves the problems in the background technology.

In order to realize the purpose, the invention is realized by the following technical scheme: cell poison gas exposes experimental facilities of infecting a virus, including closed box and receipts liquid subassembly, the inside arrangement of closed box has the culture dish, it connects in the bottom of culture dish to receive the liquid subassembly, it includes vertical pipe, electric putter, liquid push pedal, recovery tube, the first pump body and receipts liquid device to receive the liquid subassembly, the bottom of vertical pipe is fixed with electric putter, and electric putter's top is provided with the liquid push pedal that pushes away, the bottom left side of vertical pipe is connected with the recovery tube, and the recovery tube one end of keeping away from vertical pipe is connected with the first pump body, the one end that the recovery tube was kept away from to the first pump body is connected with and receives the liquid device, the inside arrangement of culture dish has the cell template, the bottom right side of vertical pipe is connected with first-flow meter valve, and the first-flow meter valve one side of keeping away from vertical pipe is connected with the transfer line, the one end that the transfer line was kept away from first-flow meter valve is connected with the second pump body, and the one end that the transfer line was kept away from the transfer line to the transfer line is connected with the culture liquid device.

Furthermore, the lateral surface of liquid pushing push pedal is laminated with the medial surface of vertical pipe mutually, and the top of vertical pipe is linked together with the bottom of culture dish.

Furthermore, the inner wall bottom of culture dish is the arc form, and the four corners position of liquid push pedal contacts with the inner wall of culture dish.

Furthermore, receive liquid subassembly still includes the piece that generates heat, the inside embedding of liquid pushing plate has the piece that generates heat.

Further, the infusion pipe is communicated with the vertical pipe through the first flowmeter valve, and the vertical pipe is communicated with the liquid collecting device through the recovery pipe.

Furthermore, a radon gas delivery pipe is arranged above the culture dish, and the right end of the radon gas delivery pipe is connected with a third pump body.

Furthermore, the bottom of the radon delivery pipe is equidistantly distributed with gas distribution branch pipes, and the bottom of each gas distribution branch pipe is connected with a second flowmeter valve.

Furthermore, the bottom of the gas distribution branch pipe is of a horn-shaped structure, and the gas distribution branch pipe and the culture dish are arranged in one-to-one correspondence.

Furthermore, the left side of the closed box is connected with a fourth pump body, the left side of the fourth pump body is connected with a first compressor, and the right side of the closed box is connected with a fifth pump body.

Furthermore, the surface of one end, close to the third pump body, of the radon gas delivery pipe is connected with a sixth pump body, and one end, far away from the radon gas delivery pipe, of the sixth pump body is connected with a second compressor.

The invention provides a cell toxic gas exposure experimental device, which has the following beneficial effects:

inside the injection principle that utilizes the liquid pushing plate to make ascending motion injects the cell culture liquid of ration into the culture dish, this setting prevents that inside the pipeline has remaining cell culture liquid when making the culture dish inside have quantitative cell culture liquid, utilizes the liquid pushing plate to make descending motion in order to retrieve the cell culture liquid that has contacted with radon gas after the experiment again to utilize the piece that generates heat to carry out high temperature disinfection along with the lift of liquid pushing plate to pipeline and culture dish inside.

1. This cell poison gas exposes experimental facilities that infects, first flowmeter valve detects cell culture liquid in the inside injection amount of vertical pipe, and the liquid pushing plate rises through electric putter and gets into the culture dish inside in order to supply the cell template to absorb in order to promote cell culture liquid, this setting is favorable to preventing that vertical intraduct has remaining cell culture liquid, is favorable to accurate providing quantitative cell culture liquid, and utilizes cell culture liquid to prevent that radon gas from getting into vertical intraduct as the separation when radon gas infects the poison experiment.

2. This cell poison gas exposes contamination experimental facilities, radon gas contamination experiment back, push away liquid push pedal decline, used cell culture liquid got into vertical intraduct along with pushing away the liquid push pedal this moment, first pump body starts to use cell culture liquid and pour into it to receiving liquid device inside with extracting vertical intraduct this moment, be favorable to retrieving used cell culture liquid with this, and cell culture liquid is retrieved the back, the piece that generates heat goes up and down through electric putter along with pushing away the liquid push pedal, the piece circular telegram that generates heat among the lift process produces high temperature in order to push away liquid push pedal self and vertical pipe, the culture dish inner wall carries out pasteurization, hidden danger harm that has with this be favorable to solving artifical the recovery.

3. This cell poison gas exposes contamination experimental facilities, the third pump body is with the radon gas along radon gas conveyer pipe, distribute gas branch pipe and distribute to the culture dish inside, so that the radon gas can contact with the cell template, so that the cell template carries out the radon gas contamination experiment, and first flowmeter valve after the experiment finishes, the third pump body is closed, the fourth pump body starts to take out the remaining radon gas that seals incasement portion, the fifth pump body seals incasement portion with external fresh air injection simultaneously, in order to keep sealing incasement portion atmospheric pressure stable, be favorable to avoiding sealing incasement portion to have the radon gas to remain simultaneously.

4. This cell poison gas exposes experimental facilities that infects poison, after the experiment, first flowmeter valve, the third pump body are closed, and the sixth pump body starts to take out with the inside radon gas of radon gas delivery pipe, gas distribution branch pipe, and take out the inside radon gas of recovery back again through the second compressor to carry to the radon gas source place, this setting is favorable to retrieving the radon gas that does not contain impurity and recycles.

Drawings

FIG. 1 is a schematic view of an interior of a closed box of the experimental apparatus for exposure of cytotoxic gas according to the present invention;

FIG. 2 is a schematic view of the external front view of the enclosure of the experimental apparatus for exposure of cytotoxic gas according to the present invention;

FIG. 3 is a schematic diagram of the internal structure of the vertical tube of the cytotoxic gas exposure apparatus according to the present invention;

FIG. 4 is an enlarged schematic view of the part A of the cytotoxic gas exposure testing apparatus of the present invention shown in FIG. 3;

FIG. 5 is a schematic structural view of the liquid-pushing plate of the cytotoxic gas exposure contamination experimental apparatus after rising;

FIG. 6 is a schematic top view of a culture dish of the experimental apparatus for cytotoxic gas exposure according to the present invention;

FIG. 7 is a schematic view of the top view of the infusion tube of the cytotoxic gas exposure experimental apparatus of the present invention.

In the figure: 1. closing the box; 2. a culture dish; 3. a liquid collection assembly; 301. a vertical tube; 302. an electric push rod; 303. pushing the liquid pushing plate; 304. a recovery pipe; 305. a first pump body; 306. a liquid collecting device; 307. a heat generating sheet; 4. a cell template; 5. a first flow meter valve; 6. a transfusion tube; 7. a second pump body; 8. a culture solution device; 9. a radon gas delivery pipe; 10. a third pump body; 11. a second flow meter valve; 12. a fourth pump body; 13. a first compressor; 14. a fifth pump body; 15. a sixth pump body; 16. a second compressor; 17. a gas distribution branch pipe.

Detailed Description

The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

As shown in fig. 1 to 7, the present invention provides a technical solution: the cell toxic gas exposure contamination experimental equipment comprises a closed box 1 and a liquid collecting assembly 3, a culture dish 2 is arranged in the closed box 1, the liquid collecting assembly 3 is connected to the bottom of the culture dish 2, the liquid collecting assembly 3 comprises a vertical pipe 301, an electric push rod 302, a liquid pushing push plate 303, a recovery pipe 304, a first pump body 305 and a liquid collecting device 306, the electric push rod 302 is fixed at the bottom of the vertical pipe 301, the liquid pushing push plate 303 is arranged at the top of the electric push rod 302, the recovery pipe 304 is connected to the left side of the bottom of the vertical pipe 301, one end, far away from the vertical pipe 301, of the recovery pipe 304 is connected with the first pump body 305, one end, far away from the recovery pipe 304, of the first pump body 305 is connected with the liquid collecting device 306, a cell template 4 is arranged in the culture dish 2, the right side of the bottom of the vertical pipe 301 is connected with a first flowmeter valve 5, one side, away from the vertical pipe 301, of the first flowmeter valve 5 is connected with a liquid conveying pipe 6, one end, away from the first flowmeter valve 5, of the liquid conveying pipe 6 is connected with a second pump body 7, one end, away from the liquid conveying pipe 6, of the second pump body 7 is connected with a culture solution device 8, the outer side surface of the liquid pushing and pushing plate 303 is attached to the inner side surface of the vertical pipe 301, the top of the vertical pipe 301 is communicated with the bottom of the culture dish 2, the bottom of the inner wall of the culture dish 2 is arc-shaped, four corners of the liquid pushing and pushing plate 303 are in contact with the inner wall of the culture dish 2, the liquid conveying pipe 6 is communicated with the vertical pipe 301 through the first flowmeter valve 5, and the vertical pipe 301 is communicated with a liquid collecting device 306 through a recovery pipe 304;

the specific operation is that the cell template 4 is placed in the culture dish 2, the four corners of the cell template 4 are in contact with the inner wall of the culture dish 2 to realize support, namely the four corners of the cell template 4 are supported by the inner wall of the culture dish 2, gaps are formed between the four sides of the cell template 4 and the inner wall of the culture dish 2 so that cell culture fluid can pass through the gaps to be in contact with the upper surface of the cell template 4, then the closed box 1 is closed to isolate the inner space and the outer space of the closed box 1 so as to prevent radon gas in the closed box 1 from being exposed during the experiment, then the cell culture fluid in the culture fluid device 8 is injected into the vertical pipe 301 along the infusion pipe 6 by the second pump body 7, in the process, the first flow meter valve 5 detects the injection amount of the cell culture fluid in the vertical pipe 301, and when the injection amount reaches a preset value, the first flow meter valve 5 closes the channel of the infusion pipe 6 to stop the injection of the cell culture fluid, at the moment, the liquid pushing plate 303 rises through the electric push rod 302 to push the cell culture liquid inside the vertical tube 301 to enter the culture dish 2 for the absorption of the cell template 4, so as to provide required nutrition for cell growth, and the cell culture liquid is used as a barrier to prevent radon gas from entering the vertical tube 301 during a radon gas contamination experiment, after the radon gas contamination experiment, the liquid pushing plate 303 descends through the electric push rod 302, at the moment, the used cell culture liquid slides down along the inner wall of the arc-shaped culture dish 2 at the bottom, the finally used cell culture liquid enters the vertical tube 301 along with the liquid pushing plate 303, at the moment, the first pump body 305 is started to extract the used cell culture liquid inside the vertical tube 301 and inject the cell culture liquid into the liquid collecting device 306 along the recovery tube 304 for temporary storage, and later-stage personnel only need to treat the cell culture liquid inside the liquid collecting device 306, thereby being beneficial to recycling the used cell culture solution and solving the hidden danger and harm to human body caused by radon gas contained in the liquid when the cell culture solution is manually recycled.

As shown in fig. 1, 3-5, the liquid receiving assembly 3 further includes a heating plate 307, and the heating plate 307 is embedded in the liquid pushing plate 303;

the operation is as follows specifically, the cell culture liquid is retrieved the back, heating plate 307 goes up and down through electric putter 302 along with liquid pushing plate 303, lift in-process heating plate 307 circular telegram produces high temperature in order to push away liquid pushing plate 303 self and vertical pipe 301, 2 inner walls of culture dish carry out pasteurization, wherein vertical pipe 301, culture dish 2 are linked together, and the produced high temperature that heating plate 307 generates heat has the diffusivity, the temperature can be followed vertical pipe 301 and transmitted to culture dish 2, thereby also can carry out pasteurization to culture dish 2 when heating plate 307 is in vertical pipe 301 inside.

As shown in fig. 1-2, a radon gas delivery pipe 9 is arranged above the culture dish 2, the right end of the radon gas delivery pipe 9 is connected with a third pump body 10, gas distribution branch pipes 17 are equidistantly distributed at the bottom of the radon gas delivery pipe 9, the bottom of the gas distribution branch pipes 17 is connected with a second flowmeter valve 11, the bottom of the gas distribution branch pipes 17 is in a horn-shaped structure, and the gas distribution branch pipes 17 are arranged in a one-to-one correspondence with the culture dish 2;

specifically, the third pump body 10 starts to distribute radon gas into the gas distribution branch pipes 17 along the radon gas delivery pipe 9 and deliver the radon gas into the culture dish 2, the radon gas contacts the cell template 4 to perform a radon gas contamination experiment, the second flowmeter valve 11 detects the radon gas amount entering the culture dish 2, and stops delivering the radon gas into the culture dish 2 when the radon gas amount flows out to reach a preset value, wherein the bottom of the gas distribution branch pipes 17 is flared so that the radon gas can be uniformly contacted with the surface of the cell template 4.

As shown in fig. 1-2, a fourth pump body 12 is connected to the left side of the closed box 1, a first compressor 13 is connected to the left side of the fourth pump body 12, a fifth pump body 14 is connected to the right side of the closed box 1, a sixth pump body 15 is connected to the surface of one end, close to the third pump body 10, of the radon gas delivery pipe 9, and a second compressor 16 is connected to one end, far away from the radon gas delivery pipe 9, of the sixth pump body 15;

the radon gas is distributed into the culture dish 2 along the radon gas delivery pipe 9 and the gas distribution branch pipe 17 by the third pump body 10, so that the radon gas can contact with the cell template 4, so that the cell template 4 can be used for a radon gas contamination experiment, after the experiment is finished, the first flow metering valve 5 is closed by the third pump body 10, the fourth pump body 12 is started to pump out residual radon gas in the closed box 1, meanwhile, the fifth pump body 14 injects external fresh air into the closed box 1 to keep the air pressure in the closed box 1 stable, meanwhile, the radon gas residual in the closed box 1 is favorably avoided, meanwhile, the first flow metering valve 5 and the third pump body 10 are closed, the sixth pump body 15 is started to pump out the radon gas in the radon delivery pipe 9 and the gas distribution branch pipe 17, the radon gas in the radon delivery pipe 9 and the gas distribution branch pipe 17 is pumped out and recovered, then compressed by the second compressor 16 and then delivered to the place where the radon gas source is located, and the configuration is favorable for recovering and recycling the radon gas without impurities.

In summary, as shown in fig. 1-fig. 7, when the experimental apparatus for exposure of cytotoxic gas is used, the cell template 4 is first placed inside the culture dish 2, and the four corners of the cell template 4 are in contact with the inner wall of the culture dish 2 to support the cell template 4, that is, the four corners of the cell template 4 are supported by the inner wall of the culture dish 2, and there are gaps between the four sides of the cell template 4 and the inner wall of the culture dish 2 for the cell culture solution to pass through, so that the cell culture solution passes through the gaps and contacts with the upper surface of the cell template 4;

then the closed box 1 is closed to isolate the inner space and the outer space of the closed box, then the cell culture solution in the culture solution device 8 is injected into the vertical pipe 301 along the liquid conveying pipe 6 by the second pump body 7, the first flow meter valve 5 detects the injection amount of the cell culture solution in the vertical pipe 301 in the process, when the injection amount reaches a preset value, the first flow meter valve 5 closes a channel of the liquid conveying pipe 6, and at the moment, the liquid pushing push plate 303 rises through the electric push rod 302 to push the cell culture solution to enter the culture dish 2 to be absorbed by the cell template 4;

then, the third pump body 10 is started to distribute radon gas into the gas distribution branch pipes 17 along the radon gas delivery pipe 9 and deliver the radon gas into the culture dishes 2, the radon gas contacts the cell template 4 to carry out a radon gas contamination experiment, the radon gas amount entering the culture dishes 2 is detected by the second flowmeter valve 11, and the radon gas is stopped from being continuously delivered into the culture dishes 2 when the radon gas amount flows out to reach a preset value;

after the experiment is finished, the first flowmeter valve 5 and the third pump body 10 are closed, a certain amount of radon gas is remained in the radon gas delivery pipe 9 and each gas distribution branch pipe 17 at the moment, and the part of radon gas is not contacted with the outside and keeps pure, so that the radon gas in the radon gas delivery pipe 9 and the gas distribution branch pipes 17 is extracted by starting the sixth pump body 15, the radon gas in the radon gas delivery pipe 9 and the gas distribution branch pipes 17 is extracted and recovered, and the extracted radon gas is compressed by the second compressor 16 and then delivered to the place where the radon gas source is located, and the arrangement is favorable for recovering and reusing the radon gas without impurities so as to avoid waste;

meanwhile, the fourth pump body 12 is started to pump out residual radon gas in the closed box 1, the radon gas containing air is compressed by the first compressor 13 and is transmitted to a designated container for storage, the designated container is a gas storage tank, and meanwhile, the fifth pump body 14 also injects outside fresh air into the closed box 1 so as to keep the gas pressure in the closed box 1 stable and be beneficial to avoiding radon gas residue in the closed box 1;

after the radon contamination experiment, the liquid pushing push plate 303 descends through the electric push rod 302, the used cell culture liquid slides down along the inner wall of the culture dish 2 with the arc-shaped bottom and enters the vertical tube 301 along with the liquid pushing push plate 303, at the moment, the first pump body 305 is started to extract the used cell culture liquid in the vertical tube 301 and inject the cell culture liquid into the liquid receiving device 306, so that the used cell culture liquid is favorably recovered, after the cell culture liquid is recovered, the heating sheet 307 is lifted up and down through the electric push rod 302 along with the liquid pushing push plate 303, the heating sheet 307 is electrified to generate high temperature in the lifting process so as to sterilize the liquid pushing push plate 303, the vertical tube 301 and the inner wall of the culture dish 2 at high temperature, wherein the vertical tube 301 and the culture dish 2 are communicated, the high temperature generated by heating of the heating sheet 307 has diffusivity, the temperature can be transmitted to the culture dish 2 from the vertical tube 301, and the culture dish 2 can also be sterilized at high temperature when the heating sheet 307 is positioned in the vertical tube 301, so that the hidden danger caused by manual recovery is favorably solved;

after all the operations are completed, the personnel need to wear protective measures, then the sealing box 1 is opened, the radon gas content is detected by using detection equipment after the sealing box 1 is opened, and the cell template 4 in the culture dish 2 is taken out after the radon gas content reaches the standard.

The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.

Claims (10)

1. Cell poison gas exposes experimental facilities of infecting with poison, including seal box (1) and receipts liquid subassembly (3), its characterized in that: a culture dish (2) is arranged in the closed box (1), the liquid collecting component (3) is connected to the bottom of the culture dish (2), the liquid collecting component (3) comprises a vertical pipe (301), an electric push rod (302), a liquid pushing push plate (303), a recovery pipe (304), a first pump body (305) and a liquid collecting device (306), an electric push rod (302) is fixed at the bottom of the vertical pipe (301), and the top of the electric push rod (302) is provided with a liquid pushing push plate (303), a recovery pipe (304) is connected to the left side of the bottom of the vertical pipe (301), and one end of the recovery pipe (304) far away from the vertical pipe (301) is connected with a first pump body (305), one end of the first pump body (305) far away from the recovery pipe (304) is connected with a liquid collecting device (306), a cell template (4) is arranged inside the culture dish (2), the right side of the bottom of the vertical pipe (301) is connected with a first flowmeter valve (5), and one side of the first flowmeter valve (5) far away from the vertical pipe (301) is connected with a transfusion pipe (6), one end of the infusion tube (6) far away from the first flowmeter valve (5) is connected with a second pump body (7), and one end of the second pump body (7) far away from the infusion tube (6) is connected with a culture solution device (8).

2. The cytotoxic gas exposure contamination experimental apparatus of claim 1, characterized in that: the outer side surface of the liquid pushing push plate (303) is attached to the inner side surface of the vertical tube (301), and the top of the vertical tube (301) is communicated with the bottom of the culture dish (2).

3. The cytotoxic gas exposure contamination experimental apparatus of claim 1, characterized in that: the bottom of the inner wall of the culture dish (2) is arc-shaped, and the four corners of the liquid pushing push plate (303) are in contact with the inner wall of the culture dish (2).

4. The cytotoxic gas exposure contamination experimental apparatus of claim 1, characterized in that: receive liquid subassembly (3) and still include heating plate (307), the inside embedding of liquid push pedal (303) has heating plate (307).

5. The cytotoxic gas exposure contamination experimental apparatus of claim 1, characterized in that: the infusion tube (6) is communicated with the vertical tube (301) through the first flowmeter valve (5), and the vertical tube (301) is communicated with the liquid collecting device (306) through the recovery tube (304).

6. The cytotoxic gas exposure contamination experimental apparatus of claim 1, characterized in that: the upper portion of culture dish (2) is provided with radon gas conveyer pipe (9), and the right-hand member of radon gas conveyer pipe (9) is connected with third pump body (10).

7. The cytotoxic gas exposure contamination experimental facility of claim 6, characterized in that: the bottom of the radon gas delivery pipe (9) is equidistantly distributed with gas distribution branch pipes (17), and the bottom of the gas distribution branch pipes (17) is connected with a second flowmeter valve (11).

8. The cytotoxic gas exposure contamination experimental facility of claim 7, characterized in that: the bottom of the gas distribution branch pipe (17) is of a horn-shaped structure, and the gas distribution branch pipe (17) and the culture dish (2) are arranged in a one-to-one corresponding mode.

9. The cytotoxic gas exposure contamination experimental apparatus of claim 1, characterized in that: the left side of sealing box (1) is connected with the fourth pump body (12), and the left side of the fourth pump body (12) is connected with first compressor (13), the right side of sealing box (1) is connected with the fifth pump body (14).

10. The cytotoxic gas exposure contamination experimental facility of claim 6, characterized in that: one end surface that radon gas delivery pipe (9) are close to third pump body (10) is connected with the sixth pump body (15), and the one end that radon gas delivery pipe (9) were kept away from to the sixth pump body (15) is connected with second compressor (16).

Images