CN115855820A - Portable integrated optical coherence tomography system and using method thereof - Google Patents
Portable integrated optical coherence tomography system and using method thereof Download PDFInfo
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- CN115855820A CN115855820A CN202211684366.6A CN202211684366A CN115855820A CN 115855820 A CN115855820 A CN 115855820A CN 202211684366 A CN202211684366 A CN 202211684366A CN 115855820 A CN115855820 A CN 115855820A
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Abstract
The invention discloses a portable integrated optical coherence tomography system and a using method thereof, wherein the system comprises a laser source, a light splitter, a reference arm, a sample arm, a spectrometer and a control device, wherein the laser source, the spectrometer, the reference arm and the sample arm are respectively connected with the light splitter, and the reference arm, the sample arm, the laser source and the spectrometer are adjacently distributed; the reference arm comprises a first collimator, a first focusing lens and a reference mirror; the sample arm comprises a second collimator, a scanning galvanometer and a focusing objective lens, and one side of the focusing objective lens is used for placing a sample to be measured. The invention adopts the improved design of integration, intellectualization and miniaturization, namely the optimized design of integration, to realize the integration, miniaturization and instrumentization of the OCT imaging system, and integrates the computer host component and the OCT system component in one frame, thereby greatly simplifying the complexity of the system and improving the integration level.
Description
Technical Field
The invention relates to a portable integrated optical coherence tomography system and a using method thereof.
Background
The OCT is a new optical diagnosis technology, light is scattered in a sample, the light returned from a reference arm and a sample arm is interfered, then is divided into interference spectrum signals with different wavelengths in a fast spectrometer, the interference spectrum signals are received by an optical linear array detector, and finally, after fast Fourier transform, the depth information of the sample can be obtained. The ability to perform high resolution, deep imaging on biological tissues has been widely used in the biomedical field, in addition, OCT has also been used in important applications in quality inspection of industrial products, and can perform nondestructive inspection and nondestructive evaluation on parts, such as fine measurement of industrial products like LCD displays, electronic chips, micro-electromechanical systems, films, coatings, and the like. Compared with other incoherent optical imaging methods, such as traditional optical microscopy, ultrasonic imaging, near-field scanning imaging, computed tomography and the like, the optical coherent tomography technology effectively makes up the defects of other technologies in the aspects of imaging depth, imaging precision and imaging speed.
However, the number of components and optical elements required by the current optical coherence tomography system are large, the distribution is dispersed, the volume is large, the system is not compact enough, the portability is poor, the integration is poor, and the light source is easily affected by static electricity.
Disclosure of Invention
The invention mainly aims to provide a portable integrated optical coherence tomography system and a using method thereof, which can perform integrated processing on each component playing a corresponding role, so that the system has small volume and is convenient to carry and deploy.
The purpose of the invention can be achieved by adopting the following technical scheme:
a portable integrated optical coherence tomography system comprises a laser source, a light splitter, a reference arm, a sample arm, a spectrometer and a control device, wherein the laser source, the spectrometer, the reference arm and the sample arm are respectively connected with the light splitter, and the reference arm, the sample arm, the laser source and the spectrometer are adjacently distributed; the reference arm comprises a first collimator, a first focusing lens and a reference mirror; the sample arm comprises a second collimator, a scanning galvanometer and a focusing objective lens, and one side of the focusing objective lens is used for placing a sample to be detected; the light of the laser light source is used for irradiating on the light splitter, one part of the light is guided to the reference reflector along the reference arm, and the other part of the light is guided to the sample to be measured along the sample arm; the scanning galvanometer is connected with a galvanometer driver, and the galvanometer driver is connected with a galvanometer power supply; the control device comprises a multifunctional digital signal I/O card, a computer mainboard and a graphic display card for image processing and display which are connected in sequence; the multifunctional digital signal I/O card is connected with the galvanometer driver and used for generating a sine wave signal for controlling the scanning galvanometer to deflect and scan and sending the sine wave signal to the galvanometer driver so that the galvanometer driver controls the scanning galvanometer deflection angle to change the laser beam path; the spectrometer is used for acquiring images fed back to the light splitter from the reference arm and the sample arm, and transmitting data to the computer mainboard through photoelectric conversion of the acquired images; the system is still including the frame, laser power supply, spectrometer, reference arm and spectrum appearance and controlling means all set up in the frame, just laser power supply and spectrum appearance all set up in one side of spectrometer, reference arm and sample arm all set up in the opposite side of spectrometer, multi-functional digital signal IO card, computer motherboard and graphic display card connect gradually from the top down and distribute in one side of spectrum appearance, the outer cladding of frame has ABS plastic casing.
Preferably, the laser light source is connected with the computer mainboard through a USB data line so as to send an enabling signal to the laser light source and control the switch of the laser light source.
Preferably, the multifunctional digital signal I/O card is connected to a computer motherboard for data exchange with the computer motherboard.
Preferably, the spectrometer comprises a CCD camera, a second focusing lens and a beam splitting grating, and a fiber collimator.
Preferably, the CCD camera of the spectrometer is connected with a computer mainboard through a USB data line so as to transmit the acquired image signal.
A method of using a portable integrated optical coherence tomography system, comprising the steps of:
step 1, the laser light source emits weak coherent light, and the weak coherent light enters a sample arm and a reference arm through a light splitter respectively to form reference light and sample light respectively;
step 2, the reference light is reflected back by a reference reflector, the sample light is transmitted to an OCT probe through an optical fiber, is collimated by a second collimator and is scanned by a scanning galvanometer, the scanning galvanometer scans a required area, the focused objective focuses a light beam, then enters a sample to be detected, is scattered by the sample, returns to a back scattering light original path, and is reflected back to interfere with the reflected reference light to form an interference light signal which enters a spectrometer and is subjected to light splitting detection;
and 3, a sensor in the CCD camera is used for acquiring an image, and then a sample tomographic image is generated through a signal processing and visualization module, so that the biological sample is imaged.
The invention has the beneficial technical effects that:
1. the invention adopts the improved design of integration, intellectualization and miniaturization, namely the optimized design of integration, to realize the integration, miniaturization and instrumentization of the OCT imaging system, and integrates the computer host component and the OCT system component in one frame, thereby greatly simplifying the complexity of the system and improving the integration level.
Drawings
FIG. 1 is a schematic diagram of a system in accordance with an embodiment of the present invention;
FIG. 2 is a schematic view of the system as a whole integrated into a frame, in accordance with an embodiment of the invention;
FIG. 3 is a schematic perspective view of a spectrometer according to an embodiment of the present invention.
In the figure: 1-laser light source, 2-beam splitter, 301-first collimator, 302-second collimator, 401-first focusing lens, 402-focusing objective lens, 5-reference reflector, 6-sample to be measured, 7-galvanometer driver, 8-galvanometer power supply, 9-scanning galvanometer, 10-spectrometer, 11-beam splitting grating, 12-second focusing lens, 13-CCD camera, 14-multifunctional digital signal I/O card, 15-computer mainboard, 16-graphic display card, 17-reflective collimator.
Detailed Description
In order to make the technical solutions of the present invention more clear and definite for those skilled in the art, the present invention is further described in detail below with reference to the examples and the accompanying drawings, but the embodiments of the present invention are not limited thereto.
As shown in fig. 1-3, the portable integrated optical coherence tomography system provided in this embodiment includes a laser source 1 (840 nmSLD laser source, center wavelength 840 nm), a spectrometer 2, a reference arm, a sample arm, a spectrometer 10, and a control device, where the laser source 1, the spectrometer 10, the reference arm, and the sample arm are respectively connected to the spectrometer 2, the reference arm and the sample arm are adjacently distributed, and the laser source 1 and the spectrometer 10 are adjacently distributed;
the reference arm comprises a first (optical fiber) collimator 301, a first focusing lens 401 and a reference reflector 5 (plane reflector), and light from the light source returns after being collimated, focused and reflected, and interferes with light reflected by the sample arm;
the sample arm comprises a second collimator 302, a scanning galvanometer 9 and a focusing objective lens 402, one side of the focusing objective lens 402 is used for placing a sample 6 to be detected, and the sample arm transmits light from a light source to the sample 6 to be detected after collimation, scanning and focusing;
the light of the laser light source 1 is irradiated on the light splitter 2, one part of the light is guided to the reference reflector 5 along the reference arm, the other part of the light is guided to the front-end optical device (OCT probe), is collimated by the second collimator and then is scanned by the scanning galvanometer 9, and then the focusing objective 402 focuses the light beam and guides the light beam to the sample 6 to be measured;
the scanning galvanometer 9 is connected with a galvanometer driver 7, and the galvanometer driver 7 is connected with a galvanometer power supply 8;
the control device comprises a multifunctional digital signal I/O card 14 (DAQ card) which is PCIE-6321, a computer mainboard 15 and a graphic display card (GPU) 16 (display card: yingwei RTX 2070) for image processing and display, wherein the multifunctional digital signal I/O card 14 is connected with the computer mainboard 15 and is used for exchanging data with the computer mainboard 15;
the multifunctional digital signal I/O card 14 is connected with the galvanometer driver 7, PCIE-6321 generates a sine wave signal for controlling the scanning galvanometer 9 to deflect and scan, the deflection angle of the galvanometer changes the path of a laser beam, and the spectrometer 10 transmits the obtained image to the computer mainboard 15 through photoelectric conversion;
the laser light source 1 is connected with a computer mainboard through a USB data line so as to send an enabling signal to the light source, thereby controlling the switch of the laser light source 1, the multifunctional digital signal I/O card is used for generating a sine wave signal for controlling the deflection scanning of the scanning galvanometer 9 and sending the sine wave signal to the galvanometer driver 7, and the galvanometer driver 7 controls the deflection angle of the scanning galvanometer 9 to change the laser beam path;
the spectrometer 10 is used for acquiring images fed back to the spectrometer 2 from the reference arm and the sample arm and transmitting data to the computer mainboard 15 through photoelectric conversion of the acquired images, the spectrometer 10 comprises a CCD camera 13, a second focusing lens 12, a light splitting grating 11 and a fiber collimator 17, and the CCD camera of the spectrometer 10 is connected with the computer mainboard through a USB data line to transmit acquired image signals.
In this embodiment, the laser light source 1 and each optical element are connected by an optical fiber assembly for transmitting the light beam of the light source to the sample arm and the reference arm.
In this embodiment, laser power supply 1, the spectrometer 2, reference arm and spectrum appearance 10 and controlling means all set up in the aluminum alloy frame, and laser power supply 1 and spectrum appearance 10 all set up in the left side of spectrometer 2, reference arm and sample arm all set up in the right side of spectrometer 2, and reference arm and sample arm interval distribution from top to bottom, multi-functional digital signal IO card 14, computer motherboard 15 and graphic display card 16 connect gradually and distribute in one side of spectrum appearance 10 from top to bottom, the outer cladding of frame has ABS plastic casing, the sample arm passes through fiber connection to aluminum alloy frame, be located outside the frame in fact, can be according to the in-service scene, the mechanical structure design of cooperation sample imaging probe, accomplish the construction of sample arm.
The working principle of the system is that an 840nmSLD weak coherent light source is used for emitting OCT scanning light beams, the weak coherent light enters a sample arm and a reference arm through light splitting, namely, the weak coherent light and the sample light are divided into reference light and sample light, the reference light is reflected back by a reflecting mirror 5 of the reference arm, the sample light is transmitted to an OCT probe through an optical fiber and is collimated by a collimator 3, a scanning vibrating mirror 9 is used for scanning, the scanning vibrating mirror 9 is used for scanning in a required area, a focusing lens 4 focuses light beams and enters a sample 6 to be detected, the light beams are scattered by the sample and returned to a back scattering light primary path, the reflected light beams interfere with the reference light to form interference light signals, the interference light signals enter a spectrometer and are detected by the light splitting, a spectrometer CCD sensor 13 is used for acquiring images, then high-resolution and high-sensitivity sample tomographic images are generated through a signal processing and visualization module, and high-resolution and high-sensitivity imaging of biological samples is realized.
In the embodiment, by integrating the computer main board, the multifunctional digital signal I/O card, the image display card and the like with the OCT system component, all functions of the existing commercial OCT system or the experimental OCT system can be realized, and the volume of the whole OCT system can be smaller, so that the OCT system can be conveniently moved, carried and deployed in a factory.
The use method of the portable integrated optical coherence tomography system comprises the following steps:
step 1, a laser light source emits weak coherent light, and the weak coherent light enters a sample arm and a reference arm through a light splitter respectively to form reference light and sample light respectively;
step 2, reflecting the reference light back through a reference reflector, transmitting the sample light to an OCT probe through an optical fiber, collimating the sample light through a second collimator, scanning the sample light through a scanning galvanometer, scanning the scanning galvanometer in a required area, focusing a light beam by a focusing objective lens, then entering the sample to be detected, scattering the sample, returning the backscattered light to an original path, and interfering the reflected reference light to form an interference light signal which enters a spectrometer and is subjected to light splitting detection;
and 3, a sensor in the CCD camera is used for acquiring an image, and then a sample tomographic image is generated through a signal processing and visualization module, so that the biological sample is imaged.
In summary, in this embodiment, the various signal generating modules and control modules required by the OCT system are integrated, intelligentized, and miniaturized, i.e. integrated optimization design, so as to realize integration, miniaturization, and instrumentation of the OCT imaging system, and meanwhile, the computer host component and the OCT system component are integrated in one frame, thereby greatly simplifying the system complexity and improving the integration level.
The above description is only for the purpose of illustrating the present invention and is not intended to limit the scope of the present invention, and any person skilled in the art can substitute or change the technical solution of the present invention and its conception within the scope of the present invention.
Claims (6)
1. A portable integrated optical coherence tomography system is characterized in that: the device comprises a laser source (1), a light splitter (2), a reference arm, a sample arm, a spectrometer (10) and a control device, wherein the laser source (1), the spectrometer (10), the reference arm and the sample arm are respectively connected with the light splitter (2), and the reference arm, the sample arm, the laser source (1) and the spectrometer (10) are adjacently distributed; the reference arm comprises a first collimator (301), a first focusing lens (401) and a reference mirror (5); the sample arm comprises a second collimator (302), a scanning galvanometer (9) and a focusing objective lens (402), and one side of the focusing objective lens (402) is used for placing a sample (6) to be measured; the light of the laser light source (1) is used for irradiating on the light splitter (2), one part of the light is guided to the reference reflector (5) along the reference arm, and the other part of the light is guided to a sample (6) to be measured along the sample arm; the scanning galvanometer (9) is connected with a galvanometer driver (7), and the galvanometer driver (7) is connected with a galvanometer power supply (8); the control device comprises a multifunctional digital signal I/O card (14), a computer mainboard (15) and a graphic display card (16) for image processing and display, which are connected in sequence; the multifunctional digital signal I/O card (14) is connected with the galvanometer driver (7), and is used for generating a sine wave signal for controlling the scanning galvanometer (9) to deflect and scan and sending the sine wave signal to the galvanometer driver (7), so that the galvanometer driver (7) controls the deflection angle of the scanning galvanometer (9) to change the laser beam path; the spectrometer (10) is used for acquiring images fed back to the spectrometer (2) from the reference arm and the sample arm, and transmitting data to the computer mainboard (15) through photoelectric conversion of the acquired images; the system is still including the frame, laser power supply (1), spectrometer (2), reference arm and spectrum appearance (10) and controlling means all set up in the frame, just laser power supply (1) and spectrum appearance (10) all set up in one side of spectrometer (2), reference arm and sample arm all set up in the opposite side of spectrometer (2), multi-functional digital signal IO card (14), computer mainboard (15) and graphic display card (16) connect gradually and distribute in one side of spectrum appearance (10) from last downwards, the outer cladding of frame has ABS plastic casing.
2. The portable integrated optical coherence tomography system of claim 1, wherein: the laser light source (1) is connected with the computer mainboard (15) through a USB data line so as to send an enabling signal to the laser light source (1) and control the laser light source (1) to be switched on and off.
3. The portable integrated optical coherence tomography system of claim 1, wherein: the multifunctional digital signal I/O card (14) is connected with the computer mainboard (15) and is used for exchanging data with the computer mainboard (15).
4. The portable integrated optical coherence tomography system of claim 1, wherein: the spectrometer (10) comprises a CCD camera (13), a second focusing lens (12), a light splitting grating (11) and a fiber collimator (17).
5. The portable integrated optical coherence tomography system of claim 5, wherein: and a CCD camera of the spectrometer (10) is connected with a computer mainboard through a USB data line so as to transmit the acquired image signal.
6. A method for using a portable integrated optical coherence tomography system, the system being as claimed in any one of claims 1 to 6, characterized in that: the using method comprises the following steps: step 1, the laser light source emits weak coherent light, and the weak coherent light enters a sample arm and a reference arm through a light splitter respectively to form reference light and sample light respectively; step 2, the reference light is reflected back by a reference reflector, the sample light is transmitted to an OCT probe through an optical fiber, is collimated by a second collimator and is scanned by a scanning galvanometer, the scanning galvanometer scans a required area, a focusing objective focuses a light beam and then enters a sample to be detected, the light beam is scattered by the sample and returns to a back scattering light original path, the reflected light is interfered with the reflected reference light, and an interference light signal is formed and enters a spectrometer to be subjected to light splitting detection; and 3, a sensor in the CCD camera is used for acquiring an image, and then a sample tomographic image is generated through a signal processing and visualization module to realize the imaging of the biological sample.
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| CN202211684366.6A CN115855820A (en) | 2022-12-27 | 2022-12-27 | Portable integrated optical coherence tomography system and using method thereof |
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| CN202211684366.6A CN115855820A (en) | 2022-12-27 | 2022-12-27 | Portable integrated optical coherence tomography system and using method thereof |
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| CN202211684366.6A Pending CN115855820A (en) | 2022-12-27 | 2022-12-27 | Portable integrated optical coherence tomography system and using method thereof |
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