CN115778888B - Preparation method of bletilla striata extract, product and application thereof - Google Patents
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Abstract
The invention relates to a preparation method of rhizoma bletillae extract, a product and application thereof, wherein the preparation method of the rhizoma bletillae extract comprises the following steps: mixing rhizoma bletilla raw material with water, performing ultrahigh pressure treatment, and performing vacuum reduced pressure reflux extraction treatment on the treated rhizoma bletilla to obtain an extract; filtering the extracting solution by using a filter cloth and performing vacuum filtration to obtain a filtrate; and freeze-drying the filtered solution to obtain the bletilla striata extract. The preparation method can make polysaccharide content in the prepared water extract at high level, and has the basis of more excellent skin moisturizing and skin repairing effects; on the other hand, compared with other extraction processes, the method can ensure that the extract can play the above effects permanently and stably to the greatest extent, so that the extract prepared by the method can be widely applied to moisturizing or repairing effect cosmetics. The preparation method is low in whole course temperature, does not adopt organic reagents in whole course, is simple and environment-friendly, and is suitable for industrial production.
Description
Technical Field
The invention belongs to the technical field of plant extraction, relates to a preparation method of a bletilla striata extract, a product and application thereof, and in particular relates to a preparation method of a bletilla striata extract with excellent skin moisturizing and repairing effects, a product and application thereof.
Background
The skin is the largest organ of the human body, providing an effective barrier between the organism and the environment, protecting us from harmful environments and also protecting us from uncontrolled water loss. Keratinocytes forming the skin barrier are the major cell type in the epidermis, accounting for about 90% of the epidermal cells, and form the skin barrier by undergoing a highly complex differentiation process involving changes in their morphological and structural integrity, known as keratinization, which affects the formation of the skin barrier and leads to skin barrier disorders, immune responses into the skin tissue, which promote the development and progression of inflammation. Related skin disorders resulting from damaged skin barriers include: atopic dermatitis, chronic actinic dermatitis, psoriasis, chloasma, acne, squamous cell carcinoma of the skin, etc., and therefore, protection and repair of the skin barrier are important.
Aquaporin-3 AQP3 plays a key role in the process of keratinocyte function, plays an important role in skin hydration, plays an important role in maintaining skin moisture and skin function, and can promote proliferation and migration of human keratinocytes, and is closely associated with the generation and repair of skin barrier.
Bletilla striata is derived from the grasses of the genus Bletillae of the family Orchidaceae, and recorded in Chinese pharmacopoeia of 2020 edition, and has the effects of astringing, stopping bleeding, detumescence and promoting granulation. Rhizoma bletilla has pharmacological activities of promoting wound healing, resisting bacteria, resisting inflammation, resisting oxidation, resisting tumor, regulating immunity, stopping bleeding, etc. Wherein the rhizoma bletilla polysaccharide is used as main component of rhizoma bletilla for promoting ulcer wound healing, resisting bacteria, stopping bleeding, etc.
At present, the traditional extraction method comprises a heating reflux extraction method, a leaching method, a decoction method and other extraction methods, such as an ultrasonic extraction method, a microwave extraction method, an enzyme-assisted extraction method and the like. Wherein the temperature of the heating extraction method is high, and the structure of the polysaccharide is easily damaged under the environment of continuous high temperature; the ultrasonic extraction method utilizes various physical mechanisms such as high-frequency oscillation, mechanical shearing effect and the like to jointly play roles, the ultrasonic has low temperature requirement, but the ultrasonic time is too long to break glycosidic bonds, so that the extraction efficiency of polysaccharide is affected; the microwave extraction method has high extraction efficiency and even heating, but the polysaccharide is gelatinized due to slightly high power; the enzyme-assisted extraction method has mild reaction conditions and low energy consumption, but has high enzyme price and strict control of the reaction conditions, otherwise, the enzyme-assisted extraction method is easy to inactivate. CN113754791a adopts an enzymolysis method to extract bletilla polysaccharide substances, the extraction conditions are strict, the enzyme activity is not easy to control, the process is complex and is not convenient for industrial production, and in the preparation method, sevage reagent is adopted, the reagent contains n-butanol and chloroform, the organic reagent has high toxicity, and great hidden trouble exists in environmental protection and safety problems.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a preparation method of a bletilla striata extract, a product and application thereof, in particular to a preparation method of a bletilla striata extract with excellent skin moisturizing and repairing effects, a product and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a method for preparing a bletilla striata extract, the method comprising the steps of:
(1) Mixing rhizoma bletilla raw material with water, performing ultrahigh pressure treatment, and performing vacuum reduced pressure reflux extraction treatment on the treated rhizoma bletilla to obtain an extract;
(2) Filtering the extracting solution by using a filter cloth and performing vacuum filtration to obtain a filtrate;
(3) And freeze-drying the filtered solution to obtain the bletilla striata extract.
The preparation method of the bletilla striata extract creatively adopts ultrahigh-pressure pretreatment and vacuum reduced pressure reflux extraction to carry out water extraction on the bletilla striata raw materials, so that the polysaccharide content in the prepared water extract is at a high level, and the preparation method has the basis of more excellent effects of moisturizing, activating skin cells, enhancing skin immunity, promoting collagen generation and accelerating skin repair; on the other hand, compared with other extraction processes (even if the polysaccharide level of the extract is equivalent), the extract can be ensured to be permanently and stably exerted on the effects to the greatest extent, has more excellent effect of promoting the expression of aquaporin-3 in epidermal cells, and has more excellent effect of promoting epidermal repair, so that the extract prepared by the preparation method can be widely applied to cosmetics with moisturizing or repairing effects. The preparation method is low in whole course temperature, does not adopt organic reagents in whole course, is simple and environment-friendly, and is suitable for industrial production.
Preferably, the rhizoma bletillae raw material is rhizoma bletillae decoction pieces, the feed liquid ratio of the rhizoma bletillae decoction pieces to water is 1 (10-20) g/mL, for example, 1:10 g/mL, 1:12 g/mL, 1:14 g/mL, 1:15 g/mL, 1:16 g/mL, 1:18 g/mL, 1:20 g/mL and the like, and other specific point values in the numerical range can be selected, so that the details are not repeated here.
The ratio of rhizoma bletillae decoction pieces to water is specifically 1 (10-20) g/mL, because if the ratio of the feed to the water is further increased, the polysaccharide content in the extract is not obviously improved, and if the ratio of the feed to the water is further reduced, the polysaccharide content in the extract is obviously reduced.
In the invention, the bletilla striata decoction pieces (dried bletilla striata tuber slices) are preferably used as the extraction raw materials, so that the problems of high viscosity of the extraction liquid and difficult solid-liquid separation caused by extracting the bletilla striata powder serving as the extraction raw materials can be avoided, and the utilization rate of the bletilla striata medicinal materials can be ensured to the greatest extent on the premise of ensuring simple and feasible preparation process.
Preferably, the pressure of the ultra-high pressure treatment is 200-350 MPa, for example 200 MPa, 220 MPa, 240 MPa, 250 MPa, 280 MPa, 300 MPa, 350 MPa, etc.; the dwell time is 4-8 min, such as 4 min, 5 min, 6 min, 7 min, 8 min, etc., and other specific values within the numerical range can be selected, and will not be described in detail herein.
When the specific technological parameters are selected by ultrahigh pressure treatment, the prepared bletilla striata extract has higher polysaccharide content, and the effectiveness of the polysaccharide can be ensured to a greater extent.
Preferably, the vacuum degree of the vacuum reduced pressure reflux extraction treatment is 0.04-0.08 MPa, for example 0.04 MPa, 0.05 MPa, 0.06 MPa, 0.07 MPa, 0.08 MPa, etc.; the heating temperature is 45-65deg.C, such as 45deg.C, 47deg.C, 50deg.C, 52 deg.C, 55deg.C, 60 deg.C, 65 deg.C, etc.; the extraction time is 30-60 min, such as 30 min, 35 min, 40 min, 45 min, 50 min, 60 min, etc.; the number of extraction is 1-4, such as 1, 2, 3, 4. Other specific point values within the above numerical ranges are all selectable, and will not be described in detail herein.
When the specific technological parameters are selected in the vacuum reduced pressure reflux extraction treatment, the prepared bletilla striata extract has higher polysaccharide content, and the polysaccharide effectiveness can be ensured to a greater extent.
Preferably, the filter cloth used for filtering the filter cloth has a pore size of 150-250 meshes, for example, 150 meshes, 170 meshes, 180 meshes, 200 meshes, 220 meshes, 240 meshes, 250 meshes, etc., and other specific point values in the numerical range can be selected, which will not be described in detail herein.
The filter aid is used for the reduced pressure suction filtration, the filter aid comprises diatomite and/or activated carbon, the consumption of the filter aid is 0.1-1% of the mass of the extracting solution, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% and the like, and other specific point values in the numerical range can be selected, so that the details are not repeated.
The filter aid used for the reduced pressure suction filtration is more preferably a combination of diatomite and active carbon, so that the quality of the filtrate is higher, and the obtained extract has more excellent moisturizing and repairing effects.
In a second aspect, the present invention provides a bletilla striata extract prepared according to the preparation method of the first aspect.
In a third aspect, the present invention provides the use of the bletilla striata extract according to the second aspect for the preparation of a cosmetic having skin moisturising and/or skin repairing effects.
In a fourth aspect, the present invention provides the use of an extract of bletilla striata according to the second aspect for the preparation of an aquaporin-3 expression promoter.
According to the research result of the invention, the bletilla striata extract prepared by the specific method has the effect of promoting the expression of the aquaporin-3, so the bletilla striata extract prepared by the specific method is used for preparing the aquaporin-3 expression promoter and plays a role in the theoretical research on the physiological action mechanism system of the aquaporin-3.
In a fifth aspect, the present invention provides a method of promoting expression of aquaporin-3 in an epidermal cell, said method comprising: administering to the epidermal cells an effective amount of the bletilla striata extract of the second aspect.
According to the research result of the invention, the bletilla striata extract prepared by the specific method has the effect of promoting the expression of aquaporin-3, so the invention also develops a method for promoting the expression of the aquaporin-3 in the epidermal cells, namely, providing the effective amount of the bletilla striata extract for scientific research.
In a sixth aspect, the present invention provides a method of promoting healing of a scratch in an epidermal cell, the method comprising: administering to the epidermal cells an effective amount of the bletilla striata extract of the second aspect.
According to the research result of the invention, the bletilla striata extract prepared by the specific method has the effect of promoting the healing of the scratches of the epidermal cells, so the invention also develops a method for promoting the healing of the scratches of the epidermal cells, namely, providing the effective amount of the bletilla striata extract to the epidermal cells for scientific research.
Compared with the prior art, the invention has the following beneficial effects:
the preparation method of the bletilla striata extract creatively provides the steps of preparing the bletilla striata extract by combining the pre-treatment of the ultrahigh pressure technology with the vacuum reduced pressure reflux extraction treatment, effectively crushing the plant cell wall by the ultrahigh pressure technology, fully entering water into cells, fully extracting active ingredients by adopting the vacuum reduced pressure reflux extraction, and on one hand, the polysaccharide content in the prepared water extract is at a high level, so that the preparation method has the foundation of more excellent moisturizing, activating skin cells, enhancing skin immunity, promoting collagen generation and accelerating skin repair effects; on the other hand, compared with other extraction processes (even if the polysaccharide level of the extract is equivalent), the extract can be ensured to be permanently and stably exerted on the effects to the greatest extent, has more excellent effect of promoting the expression of aquaporin-3 in epidermal cells, and has more excellent effect of promoting epidermal repair, so that the extract prepared by the preparation method can be widely applied to cosmetics with moisturizing or repairing effects. The preparation method is low in whole course temperature, organic reagents are not adopted in whole course, extraction conditions are mild, meanwhile, the risk problems that sticky substances are easy to gelatinize and the like due to the fact that the extraction temperature is too high in actual production are solved, and the preparation method is simple, environment-friendly and suitable for industrial production.
Drawings
FIG. 1 is a diagram showing immunofluorescence detection of aquaporin-3 expression level;
FIG. 2 is a view of a microscope for cell scratch repair test.
Detailed Description
In order to further describe the technical means adopted by the present invention and the effects thereof, the following describes the technical scheme of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The rhizoma bletillae decoction pieces related in the following examples were purchased from Yunnan Honghe river.
The HaCaT cells of the following test examples were derived from the Shanghai cell bank of the national academy of sciences.
The following method for detecting the total polysaccharide content of rhizoma bletillae (phenol sulfate method) comprises the following steps:
(1) Preparing a standard solution: preparing glucose standard solution of 0.1mg/mL, sequentially sucking 0, 0.1, 0.2, 0.4, 0.8, 1.6 and 2mL into test tube, and adding ddH 2 O was made up to 2mL in each tube for use. Preparing a sample solution: accurately weighing the sample powder, adding ddH 2 O is prepared into a sample solution of 0.1mg/mL to be measured. Preparation of 5% phenol solution: 2.5g of phenol solid powder was accurately weighed and 47.5mL of ddH was added 2 O is dissolved at 75 ℃ and stored in a dark place.
(2) Reaction of standard solution and sample solution: sequentially sucking 1mL of the prepared standard solution and sample solution, adding into a test tube, adding 0.5mL of 5% phenol solution and 2.5mL of concentrated sulfuric acid solution for reaction, and gently shaking to obtain ddH 2 O is a blank control, and the absorbance is measured at 490nm by an enzyme-labeled instrument. A standard curve was drawn with the concentration on the abscissa and the absorbance on the ordinate, and y=0.0049x+0.0018 (R 2 =0.9984). Substituting the absorbance of the sample solution into the standard curve, and calculating.
Example 1
The embodiment provides a preparation method of rhizoma bletillae extract, which comprises the following steps:
(1) Mixing rhizoma bletillae decoction pieces with water, performing ultrahigh pressure treatment under the pressure of 250 MPa for 5 min at the feed-liquid ratio of 1:15 g/mL;
(2) Carrying out vacuum reduced pressure reflux extraction on the treated mixed solution, wherein the extraction conditions are as follows: vacuum degree 0.06 MPa, temperature 50 deg.C, extraction time 60 min, total extraction 2 times;
(3) Collecting the extractive solution, filtering with 200 mesh filter cloth, adding 0.5% filter aid (diatomite and active carbon with mass ratio of 2:1), and vacuum filtering;
(4) And freeze-drying the filtrate to obtain the product.
Example 2
The embodiment provides a preparation method of rhizoma bletillae extract, which comprises the following steps:
(1) Mixing rhizoma bletillae decoction pieces with water, and performing ultrahigh pressure treatment under 300 MPa for 4 min at a feed-liquid ratio of 1:18 g/mL;
(2) Carrying out vacuum reduced pressure reflux extraction on the treated mixed solution, wherein the extraction conditions are as follows: vacuum degree 0.07 MPa, temperature 45 deg.C, extraction time 60 min, total extraction for 2 times;
(3) Collecting the extractive solution, filtering with 200 mesh filter cloth, adding 0.5% filter aid (diatomite and active carbon with mass ratio of 2:1), and vacuum filtering;
(4) And freeze-drying the filtrate to obtain the product.
Example 3
The embodiment provides a preparation method of rhizoma bletillae extract, which comprises the following steps:
(1) Mixing rhizoma bletillae decoction pieces with water, and performing ultrahigh pressure treatment under the pressure of 200 MPa for 6 min at a feed-liquid ratio of 1:12 g/mL;
(2) Carrying out vacuum reduced pressure reflux extraction on the treated mixed solution, wherein the extraction conditions are as follows: vacuum degree 0.05 MPa, temperature 60 deg.C, extraction time 50 min, total extraction 2 times;
(3) Collecting the extractive solution, filtering with 200 mesh filter cloth, adding 0.5% filter aid (diatomite and active carbon with mass ratio of 2:1), and vacuum filtering;
(4) And freeze-drying the filtrate to obtain the product.
Example 4
The present example provides a process for the preparation of bletilla striata extract, which differs from example 1 only in that the feed-to-liquid ratio in step (1) is 1:5 g/mL, the other conditions being maintained unchanged.
Example 5
This example provides a process for the preparation of bletilla striata extract which differs from example 1 only in that the pressure in step (1) is 100 MPa, the dwell time is 10 min, and the other conditions remain unchanged.
Example 6
The present example provides a process for the preparation of bletilla striata extract, which differs from example 1 only in that in step (2) the temperature is 40 ℃, the extraction time is 75 min, and the other conditions remain unchanged.
Example 7
This example provides a process for the preparation of bletilla striata extract which differs from example 1 only in that the pressure in step (1) is 500 MPa, the dwell time is 3.5 min, and the other conditions remain unchanged.
Comparative example 1
The comparative example provides a preparation method of rhizoma bletillae extract:
(1) Mixing rhizoma bletillae decoction pieces with water, wherein the feed liquid ratio is 1:15 g/mL; vacuum decompression reflux extraction is carried out, and the extraction conditions are as follows: vacuum degree 0.06 MPa, temperature 50 deg.C, extraction time 60 min, total extraction 2 times;
(2) Collecting the extractive solution, filtering with 200 mesh filter cloth, adding 0.5% filter aid (diatomite and active carbon with mass ratio of 2:1), and vacuum filtering;
(3) And freeze-drying the filtrate to obtain the product.
Comparative example 2
The comparative example provides a preparation method of rhizoma bletillae extract:
(1) Mixing rhizoma bletillae decoction pieces with water, wherein the feed liquid ratio is 1:15 g/mL; heating and reflux extracting under normal pressure, wherein the extracting conditions are as follows: extracting at 100deg.C for 60 min for 2 times;
(2) Collecting the extractive solution, filtering with 200 mesh filter cloth, adding 0.5% filter aid (diatomite and active carbon with mass ratio of 2:1), and vacuum filtering;
(3) And freeze-drying the filtrate to obtain the product.
Comparative example 3
The comparative example provides a preparation method of rhizoma bletillae extract:
(1) Mixing rhizoma bletillae decoction pieces with water, wherein the feed liquid ratio is 1:15 g/mL; soaking 12 and h, and then adopting microwave-assisted extraction under the following conditions: the microwave power is 250W, the extraction time is 30 min, and the total extraction is 2 times;
(2) Collecting the extractive solution, filtering with 200 mesh filter cloth, adding 0.5% filter aid (diatomite and active carbon with mass ratio of 2:1), and vacuum filtering;
(3) And freeze-drying the filtrate to obtain the product.
Comparative example 4
The comparative example provides a preparation method of rhizoma bletillae extract:
(1) Mixing rhizoma bletillae decoction pieces with water, wherein the feed liquid ratio is 1:15 g/mL; soaking 12 and h, and then adopting ultrasonic-enzyme assisted extraction under the following conditions: the addition amount of cellulase is 2%, the pH is 5.0, the ultrasonic power is 300W, the extraction time is 30 min, the extraction temperature is 40 ℃, and the enzyme is inactivated at 80 ℃ for 15 min after the extraction is finished;
(2) Collecting the extractive solution, filtering with 200 mesh filter cloth, adding 0.5% filter aid (diatomite and active carbon with mass ratio of 2:1), and vacuum filtering;
(3) And freeze-drying the filtrate to obtain the product.
Test example 1
The total polysaccharide content of the bletilla striata extracts prepared in examples 1 to 7 and comparative examples 1 to 4 was measured, and the results are shown in table 1:
TABLE 1
As can be seen from the data in table 1: the preparation method of the bletilla striata extract adopts the ultra-high pressure pretreatment combined with the vacuum reduced pressure reflux extraction treatment, so that the polysaccharide content in the prepared extract is at a high level, and the polysaccharide content in the extract is obviously influenced by the technological parameters of the ultra-high pressure pretreatment, the technological parameters of the vacuum reduced pressure reflux extraction treatment and the feed liquid ratio.
Test example 2
Determination of moisturizing and repair Activity on bletilla striata extracts prepared in example 1, example 7, comparative examples 2-4, with close polysaccharide levels:
(1) Moisture efficacy activity assay:
aquaporin-3 plays an important role in maintaining skin moisture and skin function, the effect of the bletilla striata extract on the expression level of the aquaporin-3 is determined by adopting an immunofluorescence method in an experiment, haCaT cells are inoculated into a 24-well plate, a normal control group and a bletilla striata extract administration group (120 mug/mL) obtained by different groups are arranged in the experiment, and the mixture is placed in an incubator (37 ℃ C., 5% CO) 2 ) Incubating 12 h, carrying out grouped drug administration when the plating rate reaches 40% -60%, incubating 24h in an incubator after the drug administration is completed, fixing cells with 4% paraformaldehyde after the incubation is completed, carrying out immunofluorescence detection after 24h, photographing by adopting a fluorescence microscope, and analyzing by using Image-Pro Ⓡ Plus Image processing software, wherein the result is shown in fig. 1. The test results are shown in table 2:
TABLE 2
As can be seen from the data in table 2: compared with the extraction process adopted in the comparative example, even though the polysaccharide content level in the extract is equivalent, the bletilla striata extract prepared by adopting the specific extraction process has remarkably excellent effect of promoting the expression of aquaporin-3. Meanwhile, the technological parameters of the ultra-high pressure pretreatment obviously influence the promotion effect on the expression of aquaporin-3.
(2) Determination of scratch repair Activity:
HaCaT cells were seeded in 12-well plates at 37℃with 5% CO 2 Culturing in a maintenance medium for 24h, placing an insert cell-forming scratch model in each well of culture plate, culturing in the culture plate for 24h, setting a normal control group (serum-free DMEM medium) and a positive control group (EGF solution concentration of EGF is 10000 IU/mL), and observing the cell growth state at scratches of 16 hours, 24 hours and 48 hours in a test substance treatment group (120 μg/mL of bletilla striata extracted from different groups) under a microscope, and recording by photographing separately, as shown in FIG. 2, and using ImageJ software calculates the healing area. The healing rate calculation formula is as follows: healing rate = (initial scratch area-scratch area to be measured time)/initial scratch area. The test results are shown in table 3:
TABLE 3 Table 3
As can be seen from the data in table 3: compared with the extraction process adopted in the comparative example, even though the polysaccharide content level in the extract is equivalent, the bletilla striata extract prepared by adopting the specific extraction process has remarkably excellent efficacy of promoting the healing and repairing of the epidermal cells. Meanwhile, the technological parameters of the ultra-high pressure pretreatment obviously influence the effects.
The applicant states that the present invention is described by way of the above examples as a method for preparing a bletilla striata extract of the present invention, and products and applications thereof, but the present invention is not limited to the above examples, i.e. it is not meant that the present invention must be practiced in dependence upon the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (8)
1. The preparation method of the bletilla striata extract is characterized by comprising the following steps of:
(1) Mixing rhizoma bletilla raw material with water, performing ultrahigh pressure treatment, and performing vacuum reduced pressure reflux extraction treatment on the treated rhizoma bletilla to obtain an extract; the pressure of the ultrahigh pressure treatment is 200-350 MPa, and the pressure maintaining time is 4-8 min;
(2) Filtering the extracting solution by using a filter cloth and performing vacuum filtration to obtain a filtrate;
(3) And freeze-drying the filtered solution to obtain the bletilla striata extract.
2. The method for preparing rhizoma bletillae extract according to claim 1, wherein the rhizoma bletillae raw material is rhizoma bletillae decoction pieces, and the feed liquid ratio of the rhizoma bletillae decoction pieces to water is 1 (10-20) g/mL.
3. The method for preparing rhizoma bletilla extract according to claim 1, wherein the vacuum degree of the vacuum reflux extraction treatment is 0.04-0.08 MPa, the heating temperature is 45-65 ℃, the extraction time is 30-60 min, and the number of times of extraction is 1-4.
4. The method for preparing rhizoma bletillae extract according to claim 1, wherein the filter cloth has a pore size of 150-250 mesh;
the decompression suction filtration uses a filter aid, wherein the filter aid comprises diatomite and/or activated carbon, and the dosage of the filter aid is 0.1-1% of the mass of the extracting solution.
5. The bletilla striata extract prepared by the preparation method according to any one of claims 1-4.
6. Use of the bletilla striata extract according to claim 5 for the preparation of cosmetics with skin moisturizing and/or skin repairing effects.
7. Use of the bletilla striata extract according to claim 5 for the preparation of aquaporin-3 expression promoters.
8. Use of the bletilla striata extract according to claim 5 for preparing a product having an effect of promoting healing of epidermal cell scratches.
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