CN115770630A - Manufacturing process method of novel three-dimensional micro mixer - Google Patents
Manufacturing process method of novel three-dimensional micro mixer Download PDFInfo
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- CN115770630A CN115770630A CN202211573790.3A CN202211573790A CN115770630A CN 115770630 A CN115770630 A CN 115770630A CN 202211573790 A CN202211573790 A CN 202211573790A CN 115770630 A CN115770630 A CN 115770630A
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Abstract
The invention discloses a manufacturing process method of a novel three-dimensional micro mixer, which can realize quick and efficient mixing and reaction among different working media; the manufacturing process comprises 4 main process steps of designing a structure mask, designing a positioning mask, assembling the mask, a stainless steel bar and a micro-channel die and manufacturing a chip main body. The invention has simple procedures in the manufacturing process, lower cost, no need of professional equipment and easy processing; the three-dimensional micro mixer fully utilizes the space of the microfluidic chip, and the mixing cavity fully utilizes the secondary flow generated by the three-dimensional structure, so that the mixing efficiency is improved, and the whole micro mixer achieves the aim of high mixing efficiency.
Description
Technical Field
The invention relates to the field of microfluidic devices, in particular to a manufacturing process method of a novel three-dimensional micro mixer.
Background
Micromixers are the most common functional components in microfluidic chip systems and are also of the greatest importance. The most basic functional component, the function of the micro mixer is to make the fluid working media with different properties reach the homogenization of the mixed components in a short time, which is the basis of all biochemical reactions, and the performance and efficiency of the biochemical reaction system taking fluid as the center are directly determined by the quality of the mass transfer mixing characteristics of the micro fluid.
The common micro-fluidic chip preparation process method at home and abroad mainly utilizes materials such as silicon base, polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA, or commonly called acrylic), glass capillary and the like, and processes the micro-fluidic chip by means of photoetching, soft photoetching, reverse molding, impressing, micro-machining, etching, 3D printing and the like; the processing period is long, the production needs professional equipment and environment, and the cost is relatively high. In the traditional manufacturing method, PMMA is mainly used as a substrate material, a channel is planned on the substrate material in a carving mode, and two same substrate materials are connected together in a bonding mode, wherein the bonding mode generally comprises a thermal bonding mode, a chemical bonding mode and the like, but any bonding mode needs professional equipment. In addition, many current scholars at home and abroad use a 3D printing mode to process a micro mixer, and form a micro channel by printing a soluble material, so that the micro channel with a complex structure can be manufactured, but due to the problem of a 3D printing process, the surface of a printed mould is uneven, smooth micro channels cannot be manufactured, and meanwhile, when a chemical agent dissolving mould is used, the micro channel is corroded to a certain degree.
Disclosure of Invention
The invention provides a manufacturing process method of a novel three-dimensional micro mixer with low cost, high efficiency and convenience, and aims to reduce the processing requirement and the processing cost of the traditional micro channel and manufacture the novel three-dimensional micro mixer by using simpler equipment.
The technical solution to achieve the above object is as follows: a manufacturing process method of a novel three-dimensional micromixer comprises the following steps:
designing and manufacturing a structure mask;
designing and manufacturing a positioning mask plate;
assembling a structural mask plate, a stainless steel bar and a micro-mixing cavity die by using a positioning mask plate, and assembling and forming a micro-mixer die;
and pouring a micro mixer die to obtain the micro mixer.
Further, the design and manufacturing of the structural mask is as follows: the structural masks are designed to be convex, convex gaps are combined with each other for installation between the masks, and the size of the gaps of the structural masks is the thickness of the processed plate.
Further, the design and manufacture of the positioning mask plate comprises the following steps: the positioning mask plate is designed into double layers, and the upper layer is designed with a positioning interval which is mainly responsible for ensuring the position relation between the stainless steel rods; the lower floor's design has fixing bolt, keeps the stability of pouring, and upper and lower floor is through gluing, leaves the passageway that can place corresponding mould.
Further, the micro-mixing cavity mold is: the mould with double helix twist structure is the equidistance between each section screw thread, and the entry requires to be parallel and level with stainless steel stick position, and the size equals.
Further, the assembling mask, the stainless steel bar and the micro mixer cavity die are as follows: assembling a structural mask plate, a stainless steel bar and a micro-mixing cavity die by using a positioning mask plate, and assembling and forming a micro-mixer die; the gap between the stainless steel bar and the micro-mixing cavity die is connected by corresponding adhesive, so that the flatness of the channel and the stability in subsequent pouring are kept.
Further, the method for manufacturing the chip main body comprises the following steps: and the micro mixer mold is used for obtaining a poured chip main body after the chip main body is solidified, pulling out the stainless steel bar, taking out the micro mixing cavity mold, and forming the micro mixer.
Compared with the prior art, the novel three-dimensional micro mixer provided by the invention has the following advantages:
1) The micro mixer has reasonable design, simple structure and low cost;
2) The inner part of the micro mixer is provided with the circular arc-shaped channel, so that the secondary flow generated by the structure is fully utilized by the mixing cavity, the mixing efficiency is improved, and the practicability of the micro mixer is effectively improved;
3) Because the whole internal channel is circular, the contact angle with the inner wall of the channel is 0 degree, and the internal channel is smooth, the contact of the reagent with the inner wall of the channel in the channel is reduced, and the generation of attachments is reduced;
4) And 3, specialized equipment is not needed, and the PDMS is directly poured to form the circular micro-channel, so that the processing requirement of the micro-fluidic chip is reduced.
Drawings
FIG. 1 is a schematic view of a mask for a micro-mixer structure according to the present invention.
Fig. 2 is a schematic view of the assembly of the mask of the micromixer structure of the present invention.
Fig. 3 is a schematic view of a micro-mixing chamber mold of the micro-mixer of the present invention.
Fig. 4 is a schematic view of a micromixer alignment mask of the present invention.
Fig. 5 is a schematic view of a micro mixer casting platform according to the present invention.
FIG. 6 is a schematic diagram of a micro-mixer according to the present invention with a partially enlarged microchannel.
Detailed Description
The invention provides a manufacturing process method of a novel three-dimensional micro mixer based on a microfluidic technology, which comprises the steps of firstly designing and manufacturing a mask plate, wherein the size of the mask plate specifies the size of the micro mixer, the position of a channel inlet and the height of a channel; using a stainless steel bar and a mould as a template for traditional PDMS (polydimethylsiloxane) pouring, inserting the stainless steel bar and the mould into corresponding positions of a mask, and connecting the stainless steel bar and the mould by using UV (ultraviolet) glue; and pouring PDMS into the mask, heating the PDMS to solidify the PDMS, and taking out the stainless steel bar and the mold to generate the micro mixer. The method comprises the following specific steps:
(1) With reference to fig. 1 and 2, the present invention designs and manufactures a specific model of a mask, and the mask will be a closed rectangle after being mounted and formed. The mask is designed to be in a convex shape, the mask is installed by combining convex-shaped gap parts, the size of the gap of the mask is the thickness of a processed plate, and the convex-shaped design facilitates the assembly of the mask and the maintenance of the coaxiality of through holes of the mask.
(2) Referring to fig. 3, the external diameter of the micro-mixing chamber model used in the present invention should be the same as the external diameter of the stainless steel rod, and the micro-mixing chamber model should be placed coaxially with the stainless steel rod.
(3) Referring to fig. 4, the present invention designs and manufactures a specific model of the positioning mask, and then the upper layer and the lower layer of the positioning mask are glued by UV glue, and the UV glue needs to be evenly applied when being applied. The passage port of the concentric shaft is penetrated by a stainless steel bar. An acrylic plate is additionally arranged under the positioning mask plate to serve as a substrate. When the UV adhesive is used, attention needs to be paid, the UV adhesive is coated on one surface of the structure mask, the surface of the structure mask coated with the UV adhesive is attached to a substrate, a certain pressure is applied to the structure mask to enable the structure mask to be attached to the substrate, and the structure mask is irradiated by ultraviolet rays for 6 minutes to enable the UV adhesive to be solidified. The structural mask is placed according to the requirements of the positioning mask.
(4) Referring to fig. 5, both ends of the stainless steel rod were polished flat with sandpaper, and the stainless steel rod and the micromixer cavity mold were ultrasonically cleaned with acetone for 5 minutes, then soaked in absolute ethanol for ultrasonic cleaning for 5 minutes, and then ultrasonically cleaned with distilled water for 5 minutes. And drying for later use. Arranging the stainless steel rods and the micro-mixing cavity die according to a positioning mask, coating UV glue on the ports of the stainless steel rods with the ports being polished flat at the joints of the stainless steel rods, sticking the UV glue on the joints, and irradiating the mask for 6 minutes by using ultraviolet rays to solidify the UV glue.
(5) Preparing PDMS, stirring uniformly, placing in a vacuum box, and vacuumizing until no bubbles emerge from the PDMS. PDMS was poured into the structural mask at the same height as the structural mask. And (3) placing the micro mixer mould in a heating box, adjusting the temperature to 70 ℃, taking out the micro mixer mould after heating for 6 hours, and taking out the stainless steel bar and the micro mixing cavity mould.
(6) Referring to fig. 6, a glass capillary tube having an outer diameter equal to that of the stainless steel rod was selected. The two ends of the glass capillary tube were polished flat with sandpaper and the surface thereof was coated with UV glue. The glass capillary was inserted into PDMS as the inlet channel, and the glass tube port was aligned with the inner wall of the channel. After alignment, the mask was irradiated with ultraviolet rays for 6 minutes to cure the UV gel. The glass capillary is connected to corresponding inlets, and different working media are input, so that mixing can be performed.
Examples
The invention provides a manufacturing process method of a novel three-dimensional micro mixer based on a microfluidic technology. Take the fabrication of a three-dimensional spiral micro-mixer as an example.
(1) With reference to fig. 1 and 2, the structural masking plates are designed to be in a convex shape, the structural masking plates can be mutually clamped and mounted through notches at two ends of the convex shape, the size of the notch of the structural masking plate is the thickness of a processed plate, and the coaxiality of the assembled structural masking plate and the through hole of the structural masking plate is kept due to the convex design. And engraving a concrete model of the structural mask on PDMS by using a laser engraving machine, wherein the main body part of the structural mask is formed by combining a mask 1, a mask 2, a mask 3 and a mask 4. The diameter of the central hole of the mask 1 is 0.6 mm, the diameter of the hole of the mask 2 is 0.6 mm, and the diameter of the hole of the mask 4 is 0.6 mm.
(2) Referring to fig. 3, the present invention uses a particular mold (9) of a micro-mixing chamber mold, the micro-mixing chamber mold (9) being indicated by the solid line portion of fig. 5, having an outer diameter of 0.6 mm and a length of 30 mm, and having a total of 11 helical segments, which are positioned coaxially with the inlet channel 5.
(3) With reference to fig. 4, a positioning mask was designed and fabricated, with a 8 cm by 8 cm design, leaving a 41 mm by 21 mm rectangle in the middle, which would be filled by the structural mask. The upper mask will leave room for the inlet 5, 6 and outlet 7 of the micromixer, but in addition some room for the mounting of the fixing means. Two holes of the M4 bolt are punched on the lower layer mask plate, the two holes are tangent to the reserved inlet position, and the bolt 8 is additionally arranged at the later stage and used for clamping the stainless steel needle. Through the combined installation of the structure masking plate and the stainless steel bar fixed structure masking plate, UV glue is coated on one surface of the structure masking plate, the surface of the main body of the structure masking plate coated with the UV glue is attached to a substrate with the thickness of 8 cm by 8 cm, a certain pressure is applied to the structure masking plate to enable the structure masking plate to be attached to the substrate, the masking plate is irradiated by ultraviolet rays for 6 minutes to enable the UV glue to be solidified, and the stainless steel bar is taken out.
(4) The two ends of the stainless steel rod are polished flat by 1000-mesh sand paper, the stainless steel rod with two polished flat ends is ultrasonically cleaned for 5 minutes by acetone, then is soaked in absolute ethyl alcohol and ultrasonically cleaned for 5 minutes, and then is ultrasonically cleaned for 5 minutes by distilled water. And drying for later use. Arranging stainless steel bars, coating UV glue on the ends of the stainless steel bars with the ends being polished flat at the joints of the stainless steel bars, sticking the stainless steel bars on the joints, and irradiating the mask with ultraviolet rays for 6 minutes to solidify the UV glue.
(5) According to the following steps: 1, stirring evenly, placing the mixture in a vacuum box, and vacuumizing until no bubbles emerge from the PDMS. PDMS is poured into the mask body, and the height of the injected amount is consistent with that of the structural mask. And (3) placing the micro mixer mold in a heating box, adjusting the temperature to 55 ℃, taking out the PDMS chip after heating for 12 hours, taking out the stainless steel bar and the glass capillary tube to generate a T-shaped micro channel, and taking out the micro mixing cavity mold to generate the micro mixer.
(6) Referring to fig. 6, a glass capillary tube with an outer diameter of 0.6 mm was selected, and both ends of the glass capillary tube were polished flat with 1000 mesh sandpaper. And coating UV glue on the surface of the glass capillary with the flat ground port, which is 5 mm away from the port. The glass capillary was inserted into PDMS as the inlet channel, and the glass tube port was aligned with the inner wall of the channel. After alignment, the UV glue was cured by UV irradiation for 6 minutes. The glass capillary is connected to corresponding inlets, and different working media are input, so that mixing can be performed.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, many modifications and refinements can be made without departing from the principle of the present invention, and these modifications and refinements should be regarded as the protection scope of the present invention.
Claims (9)
1. A manufacturing process method of a novel three-dimensional micromixer is characterized by comprising the following steps:
designing and manufacturing a structural mask;
designing and manufacturing a positioning mask plate;
assembling a structural mask plate, a stainless steel bar and a micro-mixing cavity die by using a positioning mask plate, and assembling and forming a micro-mixer die;
and pouring a micro mixer die to obtain the micro mixer.
2. The method as claimed in claim 1, wherein the design and fabrication of the structural mask comprises: the structural masks are designed to be convex, convex gaps are combined with each other for installation between the masks, and the size of the gaps of the structural masks is the thickness of the processed plate.
3. The manufacturing process of a novel three-dimensional micromixer according to claim 1, wherein said positioning mask is designed and fabricated by: the positioning mask plate is designed into double layers, and the upper layer is designed with a positioning interval to ensure the position relation between the stainless steel rods; the lower layer is provided with a fixing bolt, and the upper layer and the lower layer are glued by glue to leave a channel for placing a corresponding die.
4. A manufacturing process of a novel three-dimensional micro mixer according to claim 3, wherein the upper and lower layers of the positioning mask are adhered by UV glue.
5. The manufacturing process of the novel three-dimensional micromixer according to claim 1, wherein said micromixer cavity mold is: the mould with double helix fried dough twist structure is the equidistance between each section screw thread, and entry and stainless steel rod position parallel and level, the size equals.
6. A manufacturing process of a novel three-dimensional micro-mixer as claimed in claim 1, wherein the assembling mask and stainless steel bar and micro-mixer chamber mold are: assembling a structural mask plate, a stainless steel bar and a micro-mixing cavity die by using a positioning mask plate, and assembling and forming a micro-mixer die; the gap between the stainless steel rod and the micro-mixing chamber die is connected with an adhesive.
7. The method for manufacturing the novel three-dimensional micromixer according to claim 6, characterized in that both ends of the stainless steel bar are ground flat with sand paper, the stainless steel bar and the micromixer cavity die are ultrasonically cleaned with acetone for 5 minutes, then soaked in absolute ethyl alcohol and ultrasonically cleaned for 5 minutes, and then ultrasonically cleaned with distilled water for 5 minutes.
8. The manufacturing process method of the novel three-dimensional micro mixer according to claim 1, wherein the micro mixer is obtained by casting a micro mixer mold, and specifically comprises the following steps: and pouring a micro mixer die, obtaining a poured chip main body after the chip main body is solidified, pulling out the stainless steel bar, taking out the micro mixing cavity die, and forming the micro mixer.
9. The manufacturing process method of the novel three-dimensional micro mixer according to claim 8, wherein the casting method comprises the following steps: preparing PDMS, stirring uniformly, placing in a vacuum box, and vacuumizing until no bubbles emerge from the PDMS; pouring PDMS into the mask main body, wherein the injection amount height is consistent with the height of the structural mask; and (3) placing the micro mixer mold in a heating box, adjusting the temperature to 55 ℃, taking out the PDMS chip after heating for 12 hours, taking out the stainless steel bar and the glass capillary tube to generate a T-shaped micro channel, and taking out the micro mixing cavity mold to generate the micro mixer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211573790.3A CN115770630A (en) | 2022-12-08 | 2022-12-08 | Manufacturing process method of novel three-dimensional micro mixer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211573790.3A CN115770630A (en) | 2022-12-08 | 2022-12-08 | Manufacturing process method of novel three-dimensional micro mixer |
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| Publication Number | Publication Date |
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| CN115770630A true CN115770630A (en) | 2023-03-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202211573790.3A Pending CN115770630A (en) | 2022-12-08 | 2022-12-08 | Manufacturing process method of novel three-dimensional micro mixer |
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| CN (1) | CN115770630A (en) |
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- 2022-12-08 CN CN202211573790.3A patent/CN115770630A/en active Pending
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