CN1157694A - Method for manufacturing liquefied fish protein powder - Google Patents
Method for manufacturing liquefied fish protein powder Download PDFInfo
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- CN1157694A CN1157694A CN 96122309 CN96122309A CN1157694A CN 1157694 A CN1157694 A CN 1157694A CN 96122309 CN96122309 CN 96122309 CN 96122309 A CN96122309 A CN 96122309A CN 1157694 A CN1157694 A CN 1157694A
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- fish
- protein powder
- fish protein
- liquefied
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Abstract
A water-soluble fish protein powder is made up of deep water fish by enzymatic hydrolysis process, which includes such steps as making fish powder, degreasing, adding water and neutral protease for hydrolysis, filtering, vacuum concentration of filtrate, and spray drying. It contains more than 20 amino acids, various kinds of minerals and trace elements. It can be used as raw material to prepare baby nutritive fish powder, whole nutriment, aminoacid capsule and flavouring.
Description
The present invention relates to the method for protein hydrolysate, be specifically a kind of with the seawater fish be raw material, adopt enzymatic hydrolysis to produce the method for the extremely strong liquefied fish protein powder of solubility.
Experimental results show that: contain 8 kinds of essential amino acids of needed by human body in the flesh of fish, the essential amino acid ratio is reasonable, compares with livestock meat, and forming of it organized for a short time, and meat is soft, lysine, calcium content height.At present, vegetable proteins such as the main ether dregs of beans of the protein hydrolysate of China, peanut meal or animal blood are raw material, and adopt aquatic products or abyssal fish in the ocean as Japan, the U.S. abroad is that raw material is made the existing three kinds of formation of protein hydrolysate, i.e. FPC, function fish albumen and liquefaction fish albumen.Because liquefaction fish albumen is soluble in water, is rich in advantages such as amino acid and small peptide, so be with a wide range of applications.But the method for hydrolysis difference of liquefaction fish albumen, it is made the fish albumen composition and the characteristic that produce and also changes to some extent.
At present, the method for hydrolysis of protein mainly contains alkali process hydrolysis, acid system hydrolysis and enzymatic hydrolysis.Though the alkali process hydrolysis percent hydrolysis is higher, most of amino acid whose structures and configuration are damaged, and lose physiological action.Though the acid system hydrolysis does not destroy configuration, tryptophan is unstable to acid, and the acid system hydrolysis needs produce a large amount of sodium chloride with the NaOH neutralization, has both made desalination, and ammonification sodium content also higher (5%) has limited its range of application.
The present invention's purpose is to provide a kind of method of utilizing biological enzyme hydrolysis to make liquefied fish protein powder, and the liquefied fish protein powder of made not only is easy to become solution in water, and is rich in amino acid and small peptide and several mineral materials, trace element.
The preparation method of liquefied fish protein powder follows these steps to carry out: the first step is that the internal organ of earlier bottom fish being decaptitated are made fish meal, put into extractor then and be fish meal by weight: inertia appearance agent 1: 1～1.5 adding inertia are held agent and are carried out degreasing, extraction time 0.5-1 hour, temperature was controlled at below the boiling point.Second step was the fish meal after the degreasing to be added entry and neutral proteinase is hydrolyzed, filters, and wherein the water of Jia Ruing is 8-10 times of fish meal, and neutral proteinase is the 0.3-0.8% of solid content, hydrolysis temperature 40-80 ℃, and time 2-10 hour.The 3rd step added active carbon with filtrate filtered and carries out decoloration and deodorization, and wherein activated carbon dosage is the 70-75% of solid content in the filtrate.The 4th step: place vacuum to be concentrated into solid content the filtrate behind the decoloration and deodorization and be 40 °-45% and end.At last with concentrate through pressure spray dryer case drying, promptly can be made into white or flaxen liquefied fish protein powder.
Liquefied fish protein powder with the said method making, soluble in water, be of high nutritive value, contain 20 several amino acids, essential amino acid is formed comprehensively, content is abundant, and contain several mineral materials and trace element, dissolution characteristics (solubility, wettable, dispersivity and dissolution velocity) is better than sucrose and milk powder, and product special flavour is good, can be used for making infant nutrition fish paste, fully nutrient, amino acid gelatin pill, sports drink etc., also can be used to make the high-grade nutrient seafood condiment.It and the U.S. eat the standard of chemical code (FCC, 1983) and compare, and its physical and chemical index all meets or exceeds its standard, sees attached list.
Liquefaction fish meal protein physical and chemical index project this product index FCC index gross protein (%) 〉=80.0 〉=52.0 fat (%)≤2.0 amino-acid nitrogen (%) 〉=2.0 〉=2.0 solubility (%) 〉=99.0 〉=99.0 glutamic acid is (with C
5H
9NO
4Meter, %)≤15.0≤20.0 aspartic acid is (with C
4H
7NO
4Meter, %)≤10.0≤15.0 ash content (%)≤5.0
Accompanying drawing is a process chart of the present invention.
The present invention is further illustrated below in conjunction with embodiment shown in the drawings.
Referring to accompanying drawing, technological process of the present invention is that the internal organ of decaptitating of the bottom fish in the ocean are cleaned, blend, and drying is made fish meal then; Fish meal is put into extractor, adds the petroleum ether solvent of equivalent, and temperature is controlled at below the boiling point, and extraction time got defatted fish flour at 0.5～1 hour.
Then fish meal after the degreasing is dropped in the hydrolysis cylinder, add the water that is equivalent to 9 times of fish meal, stir, make temperature rise to 60 °-70 ℃, add solid content and be 0.5% indeterminate plant protease and be hydrolyzed, hydrolysis time is 6-8 hour, and then heat up 80 ℃ of enzymes that go out, filtration.
Filtrate filtered is squeezed in the bleaching kettle, added active carbon, be warming up to 90 °-95 ℃ then, maintain the temperature at about 0.5 hour, reduce to 50 ℃ of filtrations again.Wherein activated carbon dosage be in the filtrate solid content 75%.
Again the filtrate behind the decoloration and deodorization is squeezed in the vacuum concentration pan and to concentrate, be concentrated into solid content and be 45% and end.Vacuum when concentrating is 0.08MPa, and steam pressure is 0.15MPa.
Use the pressure spray dryer case with the concentrate spray-drying at last; Atomisation pressure 15MPa, 150 ℃ of EATs, 80 ℃ of temperature of outgoing airs, 82 ℃ of drying box temperature.After the spray-drying of above-mentioned condition, promptly get liquefied fish protein powder.
Claims (3)
1, a kind of preparation method of liquefied fish protein powder, it is characterized in that: the internal organ of earlier bottom fish being decaptitated are made fish meal, put into extractor then and add atent solvent and carry out degreasing, fish meal after the degreasing adds entry and neutral proteinase is hydrolyzed, filters, filtrate filtered adds the active carbon that accounts for the 70-75% of solid content in the filtrate again and carries out decoloration and deodorization, ends when the filtrate behind the decoloration and deodorization places vacuum to be concentrated into solid content for 40-45%; At last with concentrate through pressure spray dryer case drying, promptly get white or faint yellow liquefaction fish protein powder.
2, liquefied fish protein powder preparation method according to claim 1 is characterized in that: described atent solvent is a benzinum, its addition be fish meal weight 1-1.5 doubly, degreasing extraction time is 0.2-1 hour, temperature is controlled at below the boiling point.
3, the preparation method of liquefied fish protein powder according to claim 1 and 2 is characterized in that: during hydrolysis, the water of its adding is 8-10 times of fish meal weight, protease is plant rennet, its input amount is the 0.2-1% of solid content, hydrolysis temperature 40-80 ℃, and time 2-10 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96122309 CN1157694A (en) | 1996-10-15 | 1996-10-15 | Method for manufacturing liquefied fish protein powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96122309 CN1157694A (en) | 1996-10-15 | 1996-10-15 | Method for manufacturing liquefied fish protein powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1157694A true CN1157694A (en) | 1997-08-27 |
Family
ID=5127251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96122309 Pending CN1157694A (en) | 1996-10-15 | 1996-10-15 | Method for manufacturing liquefied fish protein powder |
Country Status (1)
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| CN (1) | CN1157694A (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1085083A4 (en) * | 1998-06-01 | 2002-11-27 | Chugai Pharmaceutical Co Ltd | Media for culturing animal cells and process for producing protein by using the same |
| CN1110258C (en) * | 2000-09-13 | 2003-06-04 | 山东大学 | Enzymolysis process for preparing new protein source |
| WO2004049818A1 (en) * | 2002-12-02 | 2004-06-17 | Green Earth Industries Llc | Apparatus and method for hydrolysis of a protein containing raw material and application of the resulting hydrolysis products |
| WO2005094618A1 (en) * | 2004-03-31 | 2005-10-13 | Wuhan Boda Fish Milk Biotechnology Co. Ltd | A process for producing fish milk |
| CN1299590C (en) * | 2005-08-25 | 2007-02-14 | 厦门大学 | Fish meal proteolytic products, its preparation process and application |
| CN100391363C (en) * | 2004-04-20 | 2008-06-04 | 大连工业大学 | River crab flavouring and its preparing method |
| CN100469252C (en) * | 2005-01-19 | 2009-03-18 | 陈洪林 | Fish noodle and its production method |
| CN101204194B (en) * | 2007-12-12 | 2010-11-10 | 宁波海浦生物科技有限公司 | Production technology of fish powder |
| CN101061827B (en) * | 2006-04-30 | 2012-01-25 | 中国食品发酵工业研究院 | Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method |
| CN104082520A (en) * | 2014-07-16 | 2014-10-08 | 常熟理工学院 | Low-value marine fish peptide meal as well as preparation method and application thereof |
| CN104115989A (en) * | 2014-07-16 | 2014-10-29 | 常熟理工学院 | Marine fish peptide powder, preparation method and applications thereof |
| CN104489602A (en) * | 2014-11-24 | 2015-04-08 | 威海市桢昊生物技术有限公司 | A method for preparing seafood seasonings using fishmeal processing wastewater |
| CN106220294A (en) * | 2016-07-07 | 2016-12-14 | 浙江海洋大学 | A kind of composite foliage fertilizer of amino-acid |
| CN106937695A (en) * | 2017-03-21 | 2017-07-11 | 舟山昌国食品有限公司 | A kind of processing method of meter of fish protein powder |
| CN107410661A (en) * | 2017-03-20 | 2017-12-01 | 舟山昌国食品有限公司 | A kind of process technology of Optimization of Low Value Fish functional protein powder |
| CN110833114A (en) * | 2013-03-15 | 2020-02-25 | 推进国际公司 | Automated method and system for recovering edible protein powder, omega-3 oil, and purified distilled water from animal tissue |
-
1996
- 1996-10-15 CN CN 96122309 patent/CN1157694A/en active Pending
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6962812B2 (en) | 1998-06-01 | 2005-11-08 | Chugai Seiyaku Kabushiki Kaisha | Culture medium for culture of animal cell and method for producing protein using same |
| US6537782B1 (en) | 1998-06-01 | 2003-03-25 | Chugai Seiyaku Kabushiki Kaisha | Media for culturing animal cells and process for producing protein by using the same |
| EP1085083A4 (en) * | 1998-06-01 | 2002-11-27 | Chugai Pharmaceutical Co Ltd | Media for culturing animal cells and process for producing protein by using the same |
| CN1110258C (en) * | 2000-09-13 | 2003-06-04 | 山东大学 | Enzymolysis process for preparing new protein source |
| US9232812B2 (en) | 2002-12-02 | 2016-01-12 | Marine Bioproducts A.S. | Apparatus and method for hydrolysis of a protein containing raw material and application of the resulting hydrolysis products |
| WO2004049818A1 (en) * | 2002-12-02 | 2004-06-17 | Green Earth Industries Llc | Apparatus and method for hydrolysis of a protein containing raw material and application of the resulting hydrolysis products |
| US8173014B2 (en) | 2002-12-02 | 2012-05-08 | Marine Bioproducts As | Apparatus for hydrolysis of a protein containing raw material and application of the resulting hydrolysis products |
| WO2005094618A1 (en) * | 2004-03-31 | 2005-10-13 | Wuhan Boda Fish Milk Biotechnology Co. Ltd | A process for producing fish milk |
| CN100391363C (en) * | 2004-04-20 | 2008-06-04 | 大连工业大学 | River crab flavouring and its preparing method |
| CN100469252C (en) * | 2005-01-19 | 2009-03-18 | 陈洪林 | Fish noodle and its production method |
| CN1299590C (en) * | 2005-08-25 | 2007-02-14 | 厦门大学 | Fish meal proteolytic products, its preparation process and application |
| CN101061827B (en) * | 2006-04-30 | 2012-01-25 | 中国食品发酵工业研究院 | Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method |
| CN101204194B (en) * | 2007-12-12 | 2010-11-10 | 宁波海浦生物科技有限公司 | Production technology of fish powder |
| CN110833114A (en) * | 2013-03-15 | 2020-02-25 | 推进国际公司 | Automated method and system for recovering edible protein powder, omega-3 oil, and purified distilled water from animal tissue |
| CN104115989A (en) * | 2014-07-16 | 2014-10-29 | 常熟理工学院 | Marine fish peptide powder, preparation method and applications thereof |
| CN104082520B (en) * | 2014-07-16 | 2016-06-01 | 常熟理工学院 | A kind of low value marine fish Gly-His-Lys and its preparation method and application |
| CN104082520A (en) * | 2014-07-16 | 2014-10-08 | 常熟理工学院 | Low-value marine fish peptide meal as well as preparation method and application thereof |
| CN104489602A (en) * | 2014-11-24 | 2015-04-08 | 威海市桢昊生物技术有限公司 | A method for preparing seafood seasonings using fishmeal processing wastewater |
| CN106220294A (en) * | 2016-07-07 | 2016-12-14 | 浙江海洋大学 | A kind of composite foliage fertilizer of amino-acid |
| CN107410661A (en) * | 2017-03-20 | 2017-12-01 | 舟山昌国食品有限公司 | A kind of process technology of Optimization of Low Value Fish functional protein powder |
| CN106937695A (en) * | 2017-03-21 | 2017-07-11 | 舟山昌国食品有限公司 | A kind of processing method of meter of fish protein powder |
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