CN115736258A - Yak bone renaturation oligopeptide powder and preparation method thereof - Google Patents
Yak bone renaturation oligopeptide powder and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of health-care product preparation, and provides yak bone double-core oligopeptide powder which comprises the following raw materials: yak bone peptide collagen peptide powder, marine oyster peptide powder, cod protein peptide powder, yeast extract and lutein crystal. The preparation method of the yak bone double-core oligopeptide powder comprises the following working steps: s1: preparing yak bone peptide collagen peptide powder; s2: preparing marine oyster peptide powder; s3: preparing cod collagen peptide; s4, preparing a yeast extract; s5, preparing lutein crystals; and S6, preparing a finished product. The yak bone peptide collagen peptide powder prepared by the method can improve bone strength, achieve the effect of preventing osteoporosis, enhance the tension and elasticity of skin, and effectively prevent melanin deposition, wherein the vitamin B2 can promote development and cell regeneration, and lutein can effectively protect eyesight, has a strong antioxidation effect, enhances the immunity of an organism, and has a delaying effect on early arteriosclerosis.
Description
Technical Field
The invention relates to the technical field of health-care product preparation, in particular to yak bone double-core oligopeptide powder and a preparation method thereof.
Background
Nowadays, people pay more and more attention to health, so people pay more and more attention to daily diet, and what is beneficial to the body is the most concerned topic of people.
Modern society develops rapidly, and diet is unreasonable, lack of motion, work and rest are irregular, sleep is not enough, mental stress, psychological stress is big, long-term bad mood etc. leads to sub-health, accelerates the health ageing, especially human skeleton and joint, and along with the increase of age, the loss of collagen, skeleton can become more fragile, and the skin also can become shriveled, the deposit of melanin etc. and along with the improvement of quality of life, hyperlipemia also becomes general health problem, along with the continuous enhancement of people's health consciousness, gradually tends to non-drug therapy.
Aiming at sub-health people, the body can be slowly conditioned through non-drug therapy, the existing health care product can only carry out maintenance physical therapy on bones, joints or skin singly, and the comprehensive maintenance of the body of people is difficult to carry out, for example, physical therapy maintenance on various aspects of the body such as bones, joints, skin and blood vessels is carried out simultaneously.
Therefore, yak bone double-core oligopeptide powder and a preparation method thereof are provided.
Disclosure of Invention
The invention mainly solves the technical problems in the prior art and provides yak bone double-core oligopeptide powder and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme that the yak bone double-core oligopeptide powder comprises the following raw materials in parts by weight: 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 15% of lutein crystal.
The preparation method of the yak bone double-core oligopeptide powder comprises the following working steps:
s1: preparing yak bone peptide collagen peptide powder;
s2: preparing marine oyster peptide powder;
s3: preparing cod collagen peptide;
s4, preparing a yeast extract;
s5, preparing lutein crystals;
s6, preparing a finished product.
Preferably, the S1 and S1 comprise a first raw material pretreatment step and a second gelatin extraction step; thirdly, separating collagen extracting solution; fourthly, compound enzymolysis; fifthly, inactivating enzyme, sterilizing and separating peptide liquid; sixthly, decoloring and deodorizing; the seventh step is concentration treatment; and eighth step, spray drying.
Preferably, the S2 and S2 comprise a first step of preparing oyster powder; secondly, size mixing; thirdly, enzymolysis; the four steps of centrifugal separation; fifthly, membrane filtration; and sixthly, heating, packaging and storing.
Preferably, the S3 and the S3 comprise a first step of raw material selection; secondly, adjusting the pH value; thirdly, crushing the raw materials; and fourthly, enzymolysis and heating treatment.
Preferably, the S4 and S4 comprise a first step of yeast impurity removal; a second step of centrifugal treatment; thirdly, preparing a suspension; step four, autolysis and heating enzyme deactivation; fifthly, centrifugal separation is carried out; and sixthly, concentrating and drying.
Preferably, the S5, S5 comprises a first step of preparing marigold pollen; the second step of preparing lutein and the third step of preparing crystal lutein.
Preferably, S6 and S6 are used for preparing finished products, and materials obtained in S1, S2, S3, S4 and S5 are fully mixed to obtain the final finished products.
Preferably, in the steps S1, S2, S3, S4 and S5, the optimum reaction time and reaction temperature are obtained by repeated experiments.
Advantageous effects
The invention provides yak bone double-core oligopeptide powder and a preparation method thereof. The method has the following beneficial effects:
(1) The yak bone peptide collagen peptide powder can promote the formation of bones and strengthen the bone collagen structure under the low calcium level, thereby improving the bone strength and achieving the effect of preventing osteoporosis, has good prevention and treatment effects on collagen such as arthropathy and the like, and improves the aging and the hypofunction of biological tissues caused by the aging, oyster is the best food for supplementing calcium, contains rich phosphorus, and is beneficial to the absorption of the calcium because the calcium needs the help of the phosphorus when being absorbed by the body, and the oyster also contains vitamin B12 which is lacked by common foods.
(2) The cod collagen peptide can repair the broken elastic fibers of the skin, enhance the tension and elasticity of the skin, thoroughly remove striae gravidarum, keep moisture required by the tender skin, effectively prevent the deposition of melanin, fade color spots, remove dark yellow and have the anti-aging effect.
(3) The yak bone renaturation oligopeptide powder and the preparation method thereof have the advantages that the yeast extract contains rich B vitamins, the vitamin B2 can promote development and cell regeneration and promote normal growth of skin, nails and hair, the vitamin B12 is an indispensable substance for promoting growth, keeping health of nervous tissues and forming normal red blood cells (erythrocytes), and can maintain normal functions of a nervous system and promote synthesis of myelin of nerve myelin.
(4) The yak bone renaturation oligopeptide powder and the preparation method thereof have the advantages that the lutein can effectively protect eyesight, has strong antioxidant effect, can inhibit the activity of active oxygen free radicals, prevent the active oxygen free radicals from damaging normal cells, enhance the immunity of an organism and have a delaying effect on early arteriosclerosis.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: the yak bone double-core oligopeptide powder comprises the following raw materials in parts by weight: 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 15% of lutein crystal.
The preparation method of the yak bone double-core oligopeptide powder comprises the following steps:
s1: preparing yak bone peptide collagen peptide powder; the S1 comprises: the first step of raw material pretreatment and the second step of gelatin extraction; thirdly, separating collagen extracting solution; fourthly, compound enzymolysis; fifthly, inactivating enzyme, sterilizing and separating peptide liquid; sixthly, decoloring and deodorizing; the seventh step is concentration treatment; and eighth step, spray drying.
The first step and the second step are that fresh or frozen yak bones are washed by high-pressure cold water to remove foreign matters, then the washed yak bones are put into a mincing machine to be crushed to 20-30mm, the crushed yak bones are placed in a clean container, the crushed yak bones are accurately measured and then put into a cooking pot, tap water with the weight 1.2 times that of the yak bones is added through a pipeline type flowmeter, a feeding port is sealed, hot water is cooked into mixed liquid containing grease and gelatin, and the cooking time is 2 hours.
The third step and the fourth step, separating collagen extracting solution; after extraction is finished, a liquid discharge valve at the bottom of the cooking tank and a feeding valve of the liquid storage tank are opened, a viewing mirror is observed, when the mixed liquid flows to a water-oil interface, the feeding valve of the liquid storage tank is closed in time, a valve of the oil storage tank is opened, and the oil is discharged into the oil storage tank, so that the oil and gelatin are complete, enzymolysis is carried out in the liquid storage tank, the hydrolysis degree of protein can be improved, more hydrolysis products with small molecular weight are obtained, the enzymolysis temperature is 48-52 ℃, and the enzymolysis time is 4-6 hours.
And in the fifth step and the sixth step, after the enzymolysis in the fourth step is finished, boiling the enzymolysis liquid for 20 minutes to inactivate the enzyme, sterilizing the soup, standing for 30 minutes, layering the hydrolysis liquid, enabling the upper layer to be pulp of non-enzymolysis macromolecular denatured protein and inactivated enzyme, enabling the lower layer to be clear and transparent peptide liquid, performing vacuum filtration, enabling the clear filtrate to enter a filtrate storage tank to obtain collagen peptidase enzymolysis liquid, enabling the collagen peptidase enzymolysis liquid to be light yellow, and performing decoloration and deodorization treatment on the enzymolysis liquid by taking powdered activated carbon as a decoloration and deodorization agent.
And in the seventh step and the eighth step, the temperature of the collagen peptide hydrolysate is controlled to be 80-82 ℃, the concentration time of the materials which are put into a concentrator in each batch is not more than 30 minutes under the vacuum degree of 0.06-0.08MPa, the materials are concentrated to 20-22 Be, the concentrated solution enters a concentrated solution storage tank for temporary storage, the material temperature is 45-50 ℃, the Baume is 20-22 Be, the air inlet temperature of a spray tower is adjusted to be 150-160 ℃, the air outlet temperature is 80-90 ℃, and the concentrated solution is dried into powder, namely the yak bone peptide collagen peptide powder.
S2: preparing marine oyster peptide powder; the S2 comprises the following steps: firstly, preparing oyster powder; secondly, size mixing; thirdly, enzymolysis; the four steps of centrifugal separation; fifthly, membrane filtration; and sixthly, heating, packaging and storing.
The first step and the second step are that fresh oysters are cleaned, drained, pulped and dried into powder, and oyster powder and purified water are mixed according to the proportion of 1:2 for later use.
Performing enzymolysis by neutral protease, wherein the amount of added enzyme is 1% of the protein content of a substrate, adding 30% of liquid alkali to adjust the pH of an enzymolysis solution to 8.0 after the enzymolysis, centrifuging for 4 hours at 50 ℃, and taking a supernatant;
and in the fifth step and the sixth step, removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight of less than 5ku, concentrating a nanofiltration membrane until the solid content reaches 10%, inactivating enzyme of the enzymatic hydrolysate in 10 minutes, performing vacuum concentration to 20% after filtering, performing spray drying (the air inlet temperature is 180 ℃ and the air outlet temperature is 80 ℃) to obtain marine oyster peptide powder, and performing vacuum packaging and low-temperature storage on the marine oyster peptide powder.
S3: preparing cod collagen peptide; s3, selecting raw materials in the first step; secondly, adjusting the pH value; thirdly, crushing the raw materials; and fourthly, enzymolysis and heating treatment.
Selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of calcium hydroxide and sodium hydroxide is 0.2-0.5%, the weight ratio of calcium hydroxide to sodium hydroxide is 1:2-4, repeatedly washing with clear water to enable the pH value of the cod skin to be neutral, adding a dilute acid aqueous solution with the concentration of 0.3-0.6%, soaking for 1-3 hours, and repeatedly washing with clear water to enable the pH value of the cod skin to be neutral;
and step three and step four, crushing the fish skin, stirring and extracting for 8-10 hours in hot water at the temperature of 70-80 ℃, filtering to remove insoluble substances to obtain a collagen extracting solution, adjusting the pH value of the collagen extracting solution to be neutral, adding enzyme for enzymolysis for 3-5 hours under the stirring condition, heating to 80 ℃ at the enzymolysis temperature of 40-50 ℃, inactivating the enzyme in the enzymolysis solution for 10 minutes, filtering, concentrating the concentration in vacuum to 20%, and performing spray drying (the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃) to obtain the cod collagen peptide.
S4, preparing a yeast extract; s4, removing impurities from the yeast in a first step; a second step of centrifugal treatment; thirdly, preparing a suspension; fourthly, autolysis and heating enzyme deactivation are carried out; fifthly, centrifugal separation; and sixthly, concentrating and drying.
The first step and the second step, sieving with a 200-mesh sieve to remove impurities in the yeast, washing off some impurities such as ash, emulsified substances and the like to obtain a yeast solution, deodorizing the yeast with five-thousandth weak alkaline solution, centrifuging for 30min at the rotating speed of 3500-5000r/min to obtain yeast paste, and removing non-yeast components dissolved in water;
preparing the yeast paste obtained in the second step into 10-15% yeast suspension, carrying out autolysis for 24-28h under the conditions of pH5.5-6.5 and temperature 40-55 ℃, and after the autolysis is finished, heating to 90-95 ℃ for 15-20min to inactivate enzymes in yeast cells;
and in the fifth step and the sixth step, the enzyme-deactivated semi-finished product is cooled, centrifuged for 30min at the rotating speed of 3500-5000r/min to obtain autolyzed supernatant, the autolyzed supernatant obtained by centrifugation is concentrated under reduced pressure at the temperature of 60 ℃ to the dry matter concentration of 40 percent (semi-solid state), and then the autolyzed supernatant is sprayed and dried into powder under certain conditions (the air inlet temperature is 165 ℃ and the air outlet temperature is 90 ℃) to obtain the powdery yeast extract.
S5, preparing lutein crystals; s5 comprises the first step of preparing marigold pollen; the second step of preparing lutein and the third step of preparing crystal lutein.
Taking petals of fresh marigold flowers, performing light-resistant fermentation treatment, adding liquid nitrogen, and grinding into powder (40-60 um) to obtain marigold pollen;
in the second step, the lutein crude product is dissolved in a small amount of tetrahydrofuran (containing 0.01 percent of BHT), water is added until the solution becomes turbid, the mixture is stirred under the condition of shading and sealing at room temperature to recrystallize the compound, then solid is centrifugally separated, the solid is washed by water to remove the tetrahydrofuran, and finally the lutein crystal is obtained after vacuum drying at 0 ℃;
and the third step, dissolving the lutein crude product in a small amount of tetrahydrofuran (containing 0.01 percent of BHT), adding water until the solution becomes turbid, stirring under the conditions of shading and sealing at room temperature to recrystallize the compound, then centrifugally separating out solid, washing the solid with water to remove the tetrahydrofuran, and finally performing vacuum drying at 0 ℃ to obtain the lutein crystal.
S6, preparing a finished product; and fully mixing the materials obtained in the steps S1, S2, S3, S4 and S5 to obtain a final finished product.
Example two: the yak bone double-core oligopeptide powder comprises the following raw materials in parts by weight: 40% of yak bone peptide collagen peptide powder, 25% of marine oyster peptide powder, 20% of cod protein peptide powder, 10% of yeast extract and 5% of lutein crystal.
The preparation method of the yak bone renaturation oligopeptide powder comprises the following steps:
s1: preparing yak bone peptide collagen peptide powder; the S1 comprises: firstly, pretreating raw materials and secondly, extracting gelatin; thirdly, separating collagen extracting solution; fourthly, compound enzymolysis; fifthly, inactivating enzyme, sterilizing and separating peptide liquid; sixthly, decoloring and deodorizing; the seventh step is concentration treatment; and eighth step, spray drying.
Washing fresh or frozen yak bones with high-pressure cold water to remove foreign matters, putting the washed yak bones into a mincing machine, crushing the yak bones to 20-30mm, putting the yak bones into a clean container, accurately metering the crushed yak bones, putting the yak bones into a cooking pot, adding tap water which is 1.2 times of the weight of the yak bones through a pipeline type flowmeter, sealing a feeding port, cooking the yak bones with hot water to form a mixed solution containing grease and gelatin, wherein the cooking time is 2 hours.
The third step and the fourth step, separating collagen extracting solution; after extraction, a liquid discharge valve at the bottom of the cooking tank and a feeding valve of the liquid storage tank are opened, a viewing mirror is observed, when the mixed liquid flows to a water-oil interface, the feeding valve of the liquid storage tank is closed in time, a valve of the oil storage tank is opened, and the oil is discharged into the oil storage tank, so that the oil and gelatin are complete, enzymolysis is carried out in the liquid storage tank, the hydrolysis degree of protein can be improved, more hydrolysate with small molecular weight is obtained, and the enzymolysis temperature is 48-52 ℃ and the time is 4-6 hours.
And in the fifth step and the sixth step, after the enzymolysis in the fourth step is finished, boiling the enzymolysis liquid for 20 minutes to inactivate the enzyme, sterilizing the soup, standing for 30 minutes, layering the hydrolysis liquid, enabling the upper layer to be pulp of non-enzymolysis macromolecular denatured protein and inactivated enzyme, enabling the lower layer to be clear and transparent peptide liquid, performing vacuum filtration, enabling the clear filtrate to enter a filtrate storage tank to obtain collagen peptidase enzymolysis liquid, enabling the collagen peptidase enzymolysis liquid to be light yellow, and performing decoloration and deodorization treatment on the enzymolysis liquid by taking powdered activated carbon as a decoloration and deodorization agent.
And in the seventh step and the eighth step, the temperature of the collagen peptide hydrolysate is controlled to be 80-82 ℃, the concentration time of the materials which are put into a concentrator in each batch is not more than 30 minutes under the vacuum degree of 0.06-0.08MPa, the materials are concentrated to 20-22 Be, the concentrated solution enters a concentrated solution storage tank for temporary storage, the material temperature is 45-50 ℃, the Baume is 20-22 Be, the air inlet temperature of a spray tower is adjusted to be 150-160 ℃, the air outlet temperature is 80-90 ℃, and the concentrated solution is dried into powder, namely the yak bone peptide collagen peptide powder.
S2: preparing marine oyster peptide powder; the S2 comprises the following steps: firstly, preparing oyster powder; secondly, size mixing; thirdly, enzymolysis; the four steps of centrifugal separation; fifthly, membrane filtration; and sixthly, heating, packaging and storing.
The first step and the second step are that fresh oysters are cleaned, drained, pulped and dried into powder, and oyster powder and purified water are mixed according to the proportion of 1:2 for later use.
In the third step and the fourth step, neutral protease is used for enzymolysis, the enzyme adding amount is 1 percent of the protein content of the substrate, after the enzymolysis, 30 percent of liquid caustic soda is added to adjust the pH value of the enzymolysis liquid to 8.0, the enzymolysis liquid is centrifuged for 4 hours at the temperature of 50 ℃, and the supernatant is taken;
and in the fifth step and the sixth step, removing impurities by using a ceramic membrane, filtering by using an ultrafiltration membrane to obtain polypeptide with the molecular weight of less than 5ku, concentrating a nanofiltration membrane until the solid content reaches 10%, inactivating enzyme of the enzymatic hydrolysate in 10 minutes, performing vacuum concentration to 20% after filtering, performing spray drying (the air inlet temperature is 180 ℃ and the air outlet temperature is 80 ℃) to obtain marine oyster peptide powder, and performing vacuum packaging and low-temperature storage on the marine oyster peptide powder.
S3: preparing cod collagen peptide; s3, selecting raw materials in the first step; secondly, adjusting the pH value; thirdly, crushing the raw materials; and fourthly, enzymolysis and heating treatment.
Selecting cod skin as a raw material, washing the cod skin with clear water, adding a mixed solution of calcium hydroxide and sodium hydroxide, soaking for 8-14 hours, wherein the concentration of the mixed solution of calcium hydroxide and sodium hydroxide is 0.2-0.5%, the weight ratio of calcium hydroxide to sodium hydroxide is 1:2-4, repeatedly washing with clear water to enable the pH value of the cod skin to be neutral, adding a dilute acid aqueous solution with the concentration of 0.3-0.6%, soaking for 1-3 hours, and repeatedly washing with clear water to enable the pH value of the cod skin to be neutral;
and step three and step four, crushing the fish skin, stirring and extracting for 8-10 hours in hot water at the temperature of 70-80 ℃, filtering to remove insoluble substances to obtain a collagen extracting solution, adjusting the pH value of the collagen extracting solution to be neutral, adding enzyme for enzymolysis for 3-5 hours under the stirring condition, heating to 80 ℃ at the enzymolysis temperature of 40-50 ℃, inactivating the enzyme in the enzymolysis solution for 10 minutes, filtering, concentrating the concentration in vacuum to 20%, and performing spray drying (the air inlet temperature is 180 ℃, the air outlet temperature is 80 ℃) to obtain the cod collagen peptide.
S4, preparing a yeast extract; s4, removing impurities from the yeast in a first step; a second step of centrifugal treatment; thirdly, preparing a suspension; step four, autolysis and heating enzyme deactivation; fifthly, centrifugal separation is carried out; and sixthly, concentrating and drying.
The first step and the second step, sieving with a 200-mesh sieve to remove impurities in the yeast, washing off some impurities such as ash, emulsified substances and the like to obtain a yeast solution, deodorizing the yeast with five-thousandth weak alkaline solution, centrifuging for 30min at the rotating speed of 3500-5000r/min to obtain yeast paste, and removing non-yeast components dissolved in water;
preparing the yeast paste obtained in the second step into 10-15% yeast suspension, carrying out autolysis for 24-28h under the conditions of pH5.5-6.5 and temperature 40-55 ℃, and after the autolysis is finished, heating to 90-95 ℃ for 15-20min to inactivate enzymes in yeast cells;
and in the fifth step and the sixth step, cooling the enzyme-inactivated semi-finished product, centrifuging for 30min at the rotating speed of 3500-5000r/min to obtain autolyzed supernatant, concentrating the autolyzed supernatant obtained by centrifuging at the temperature of 60 ℃ under reduced pressure to obtain dry matter concentration of 40% (semi-solid state), and then spraying and drying into powder under certain conditions (the air inlet temperature of 165 ℃ and the air outlet temperature of 90 ℃) to obtain the powdery yeast extract.
S5, preparing lutein crystals; s5, preparing marigold pollen in a first step; the second step of preparing lutein and the third step of preparing crystal lutein.
Taking petals of fresh marigold flowers, performing light-resistant fermentation treatment, adding liquid nitrogen, and grinding into powder (40-60 um) to obtain marigold pollen;
in the second step, the crude xanthophyll is dissolved in a small amount of tetrahydrofuran (containing 0.01% of BHT), water is added until the solution becomes turbid, the mixture is stirred under a sealed condition of shading and shielding at room temperature to recrystallize the compound, then the solid is centrifugally separated, the solid is washed with water to remove the tetrahydrofuran, and finally the dried product is vacuum-dried at 0 ℃ to obtain xanthophyll crystals;
and the third step, dissolving the lutein crude product in a small amount of tetrahydrofuran (containing 0.01 percent of BHT), adding water until the solution becomes turbid, stirring under the conditions of shading and sealing at room temperature to recrystallize the compound, then centrifugally separating out solid, washing the solid with water to remove the tetrahydrofuran, and finally performing vacuum drying at 0 ℃ to obtain the lutein crystal.
S6, preparing a finished product; and fully mixing the materials obtained in the steps S1, S2, S3, S4 and S5 to obtain a final finished product.
The yak bone peptide collagen peptide powder can promote the formation of bones and strengthen the bone collagen structure under the low calcium level, thereby improving the bone strength, achieving the effect of preventing osteoporosis, having good prevention and treatment effects on collagen such as arthrosis and the like, and improving the aging and hypofunction of biological tissues caused by the aging; oyster is the best food for supplementing calcium, contains rich phosphorus, is beneficial to calcium absorption because the calcium is absorbed by the body by the aid of the phosphorus, and also contains vitamin B12 which is lacked by common foods; the cod collagen peptide can repair the broken elastic fibers of the skin, enhance the tension and elasticity of the skin, thoroughly remove striae gravidarum, keep moisture required by the tender skin, effectively prevent the deposition of melanin, fade color spots, remove dark yellow and have the anti-aging effect; the yeast extract contains rich B vitamins, vitamin B2 can promote development and cell regeneration, promote normal growth of skin, nail and hair, vitamin B12 is an indispensable substance for promoting growth, keeping health of nerve tissues and forming normal red blood cells (erythrocytes), can maintain normal functions of the nervous system and promote synthesis of myelin of nerve myelin; the lutein can effectively protect eyesight, has strong antioxidation effect, can inhibit the activity of active oxygen free radicals, prevent the active oxygen free radicals from damaging normal cells, enhance the immunity of an organism, and has a delaying effect on early arteriosclerosis.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (9)
1. Yak bone double-core oligopeptide powder, its characterized in that: the feed comprises the following raw materials in parts by weight: 40% of yak bone peptide collagen peptide powder, 20% of marine oyster peptide powder, 20% of cod protein peptide powder, 5% of yeast extract and 15% of lutein crystal.
2. The preparation method of the yak bone double-core oligopeptide powder comprises the following working steps:
s1: preparing yak bone peptide collagen peptide powder;
s2: preparing marine oyster peptide powder;
s3: preparing cod collagen peptide;
s4, preparing a yeast extract;
s5, preparing lutein crystals;
and S6, preparing a finished product.
3. The preparation method of the yak bone double-core oligopeptide powder according to claim 2, which is characterized by comprising the following steps: the S1 and S1 comprise the steps of raw material pretreatment and gelatin extraction in the second step; thirdly, separating collagen extracting solution; fourthly, compound enzymolysis; fifthly, inactivating enzyme, sterilizing and separating peptide liquid; sixthly, decoloring and deodorizing; the seventh step is concentration treatment; and eighth step, spray drying.
4. The preparation method of the yak bone double-core oligopeptide powder according to claim 2, which is characterized by comprising the following steps: s2 and S2 comprise the first step of preparing oyster powder; secondly, size mixing; thirdly, enzymolysis; the four steps of centrifugal separation; fifthly, membrane filtration; and sixthly, heating, packaging and storing.
5. The preparation method of the yak bone double-core oligopeptide powder according to claim 2, which is characterized by comprising the following steps: s3, S3 comprises the first step of raw material selection; secondly, adjusting the pH value; thirdly, crushing the raw materials; and fourthly, enzymolysis and heating treatment.
6. The preparation method of the yak bone double-core oligopeptide powder according to claim 2, which is characterized by comprising the following steps: s4, S4 comprises a first step of yeast impurity removal; a second step of centrifugal treatment; thirdly, preparing a suspension; fourthly, autolysis and heating enzyme deactivation are carried out; fifthly, centrifugal separation is carried out; and sixthly, concentrating and drying.
7. The preparation method of the yak bone double-core oligopeptide powder according to claim 2, which is characterized by comprising the following steps: s5 and S5 comprise the first step of preparing marigold pollen; the second step of preparing lutein and the third step of preparing crystal lutein.
8. The preparation method of the yak bone double-core oligopeptide powder according to claim 2, which is characterized by comprising the following steps: and S6 are used for preparing finished products, and the materials obtained in S1, S2, S3, S4 and S5 are fully mixed to obtain the final finished product.
9. The preparation method of the yak bone double-core oligopeptide powder according to claim 2, which is characterized by comprising the following steps: in the processes of S1, S2, S3, S4 and S5, the optimal reaction time and reaction temperature are obtained through repeated experiments.
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| CN103783254A (en) * | 2013-07-02 | 2014-05-14 | 吴长海 | Preparation method of yak bone collagen peptide |
| CN104256657A (en) * | 2014-09-01 | 2015-01-07 | 王书敏 | Quaternary compound peptide powder for repairing human cells |
| CN108967800A (en) * | 2018-08-13 | 2018-12-11 | 蔡艳霞 | A kind of functional solid beverage and preparation method thereof |
| CN110720636A (en) * | 2019-11-18 | 2020-01-24 | 大连非得生物产业有限公司 | Yak bone renaturation oligopeptide powder and preparation method thereof |
| CN111574618A (en) * | 2020-06-26 | 2020-08-25 | 润科生物工程（福建）有限公司 | Preparation method of whitening compound protein peptidase hydrolysate, whitening compound protein peptide beverage and preparation method thereof |
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| CN103783254A (en) * | 2013-07-02 | 2014-05-14 | 吴长海 | Preparation method of yak bone collagen peptide |
| CN104256657A (en) * | 2014-09-01 | 2015-01-07 | 王书敏 | Quaternary compound peptide powder for repairing human cells |
| CN108967800A (en) * | 2018-08-13 | 2018-12-11 | 蔡艳霞 | A kind of functional solid beverage and preparation method thereof |
| CN110720636A (en) * | 2019-11-18 | 2020-01-24 | 大连非得生物产业有限公司 | Yak bone renaturation oligopeptide powder and preparation method thereof |
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