CN115735716A - Culture medium and preparation method thereof - Google Patents

Culture medium and preparation method thereof Download PDF

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Publication number
CN115735716A
CN115735716A CN202211471749.5A CN202211471749A CN115735716A CN 115735716 A CN115735716 A CN 115735716A CN 202211471749 A CN202211471749 A CN 202211471749A CN 115735716 A CN115735716 A CN 115735716A
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chinese torreya
parts
buffer solution
shells
culture medium
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Inventor
郭彬
裘高扬
刘俊丽
李华
傅庆林
陈晓冬
王鸢
刘琛
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Zhejiang Academy of Agricultural Sciences
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Zhejiang Academy of Agricultural Sciences
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The invention belongs to the technical field of seedling raising substrates, and particularly relates to a culture medium and a manufacturing method thereof. The invention provides a cultivation substrate and a preparation method thereof, aiming at the problem of recycling Chinese torreya shells in the prior art, wherein the cultivation substrate comprises the Chinese torreya shells, vermiculite particles, perlite, bagasse, a buffer solution and a particle slow-release fertilizer. The invention successfully provides a culture medium using Chinese torreya shells as a main raw material, realizes resource recycling of the Chinese torreya shells, and simultaneously, compared with the scheme of using sulfuric acid to acidify and neutralize alkalinity in the prior art, the invention uses acetic acid-sodium acetate as a buffer solution, and is more suitable for the growth of rice plants.

Description

Culture medium and preparation method thereof
Technical Field
The invention relates to the technical field of seedling culture substrates, in particular to a culture substrate and a manufacturing method thereof.
Background
Chinese torreya needs to be subjected to a plurality of processes such as peeling, piling, drying in the sun, frying and the like from Chinese olive to dry fruit, while the Chinese torreya green coat shell which is peeled in the past is mostly abandoned as agricultural garbage, which causes environmental pollution. Therefore, how to recycle the Chinese torreya shells into resources is one of the problems to be solved in the prior art.
The use of such hulls as a substrate material for growing seedlings is one of the commonly used ways for recycling, and for example, the preparation of a rice seedling substrate from pecan shells is well documented in the prior art. However, researches show that the Chinese torreya shells are used as seedling raising substrates, the Chinese torreya shells are soft in texture and easy to collapse, and a large amount of alkaloid is contained in the Chinese torreya shells, which are two main reasons for preventing the Chinese torreya shells from being applied to the seedling raising substrates on a large scale.
For example, the chinese invention patent application discloses a rice seedling raising substrate [ application No.: 201510925518.0], the invention patent application comprises the following components: 15-25 parts of sieved algae mud charcoal soil, 5-12 parts of rice chaff carbon, 5-12 parts of deep mountain humus, 8-16 parts of selenium-rich mineral substances, 6-16 parts of decomposed straw powder and 5-12 parts of traditional Chinese medicine residues; the traditional Chinese medicine residues are a mixture of pine needles, radix stemonae, eupatorium adenophorum, dendrobium, liquorice and codonopsis pilosula, and are subjected to high-temperature aerobic fermentation treatment, and the thoroughly decomposed straw powder is prepared by drying fresh straws, crushing into 1-2mm thoroughly decomposed straw powder, watering, covering with a plastic film, preserving moisture, stacking at the ambient temperature of 20-35 ℃ in the sun, and fermenting until all the straws become brownish black.
The invention has the advantages of proper matrix component proportion, excellent physicochemical property, good permeability and strong water and fertilizer retention capacity, can meet the requirements of oxygen, nutrition and moisture required by seedlings, but cannot reasonably and effectively apply the Chinese torreya shells.
Disclosure of Invention
The invention aims to solve the problems and provides a culture medium taking Chinese torreya shells as a main raw material.
Another object of the present invention is to provide a method for preparing a cultivation substrate using torreya grandis shells as a main raw material.
The purpose of the invention is realized by the following technical scheme:
a culture medium comprises Chinese torreya shells, vermiculite particles, perlite, bagasse, buffer solution and particle slow release fertilizer.
In the culture medium, the culture medium comprises 80-120 parts by weight of Chinese torreya shells, 10-30 parts by weight of vermiculite particles, 10-20 parts by weight of perlite, 2-6 parts by weight of bagasse, 8-12 parts by weight of buffer solution and 0.15-0.25 part by weight of particle slow release fertilizer.
In the above culture substrate, the: the culture medium comprises, by mass, 100 parts of Chinese torreya shells, 20 parts of vermiculite particles, 15 parts of perlite, 4 parts of bagasse, 10 parts of buffer solution and 0.2 part of particle slow release fertilizer.
In the culture medium, the buffer solution is acetic acid-sodium acetate buffer solution.
In the culture medium, the acetic acid-sodium acetate buffer solution is prepared by the following method: taking 18g of sodium acetate and 9.8ml of glacial acetic acid, and adding water to dilute to 1000ml to obtain an acetic acid-sodium acetate buffer solution.
A preparation method of a culture medium comprises the following steps:
the method comprises the following steps: collecting and drying the Chinese torreya shell raw material;
step two: crushing the dried Chinese torreya shell raw material by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution, and soaking;
step four: then adding perlite, vermiculite particles, bagasse and particle slow-release fertilizer, uniformly mixing, and carrying out screw extrusion forming to obtain the culture medium.
In the preparation method of the culture medium, after drying in the first step, the water content of the Chinese torreya shell raw material is less than 20%.
In the preparation method of the culture medium, the particle size of the Chinese torreya shells selected in the step two is 2-20mm.
In the preparation method of the culture medium, the pH value of the acetic acid-sodium acetate buffer solution in the third step is 4-5.
In the preparation method of the culture medium, the soaking time in the third step is 20-40min.
Compared with the prior art, the invention has the advantages that:
1. the invention successfully provides a cultivation medium using Chinese torreya shells as a main raw material, and realizes resource recycling of the Chinese torreya shells.
2. Compared with the scheme of using sulfuric acid to acidify and neutralize alkalinity in the prior art, the method is more suitable for plant growth.
3. According to the invention, a screw extrusion technology is adopted to extrude the Chinese torreya shell hulls, so that the material is more compact and is not easy to enter water, thus the decomposition of the Chinese torreya shell hulls is reduced, and the durability of the material is improved. And vermiculite particles, perlite, bagasse and other materials which are not easy to degrade are added, so that the practicability of the whole matrix product is enhanced.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments.
Example 1
The embodiment provides a culture medium which comprises, by mass, 80 parts of Chinese torreya shells, 30 parts of vermiculite particles, 20 parts of perlite, 6 parts of bagasse, 12 parts of acetic acid-sodium acetate buffer solution and 0.25 part of particle slow-release fertilizer.
Wherein, the acetic acid-sodium acetate buffer solution can adopt the preparation method of the commonly prepared buffer solution in the prior art, namely the preparation method of taking acetic acid and sodium acetate with a certain proportion in a container and adding water to dilute to the preset concentration.
Example 2
The embodiment provides a culture medium which comprises, by mass, 120 parts of Chinese torreya shells, 10 parts of vermiculite particles, 10 parts of perlite, 2 parts of bagasse, 8 parts of acetic acid-sodium acetate buffer solution and 0.15 part of particle slow-release fertilizer.
Wherein, the acetic acid-sodium acetate buffer solution can adopt the preparation method of the commonly prepared buffer solution in the prior art, namely the preparation method of taking acetic acid and sodium acetate with a certain proportion in a container and adding water to dilute to the preset concentration.
Example 3
The embodiment provides a culture medium which comprises, by mass, 100 parts of Chinese torreya shells, 20 parts of vermiculite particles, 15 parts of perlite, 4 parts of bagasse, 10 parts of acetic acid-sodium acetate buffer solution and 0.2 part of particle slow release fertilizer.
Wherein, the acetic acid-sodium acetate buffer solution can adopt the preparation method of the commonly prepared buffer solution in the prior art, namely the preparation method of taking acetic acid and sodium acetate with a certain proportion in a container and adding water to dilute to the preset concentration.
Example 4
The embodiment provides a preparation method of a culture substrate, which comprises the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of 2-20mm, crushing the dried Chinese torreya shells by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution with pH of 4, and soaking for 20min; in the embodiment, the particle diameter of the Chinese torreya shell powder is 20-100 meshes, preferably 80 meshes, and the following steps are carried out;
step four: then adding perlite, vermiculite particles, bagasse and granular slow-release fertilizer, uniformly mixing, and performing screw extrusion forming to obtain granules with the grain diameter of 2-5mm to obtain the culture medium.
Example 5
The embodiment provides a preparation method of a culture medium, which comprises the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of 2-20mm, crushing the dried Chinese torreya shell raw material by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Torreya grandis shell powder with acetic acid-sodium acetate buffer solution with pH of 5, and soaking for 40min;
step four: then adding perlite, vermiculite particles, bagasse and particle slow-release fertilizer, uniformly mixing, and carrying out screw extrusion forming to obtain the culture medium.
Example 6
The embodiment provides a preparation method of a culture medium, which comprises the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of 2-20mm, crushing the dried Chinese torreya shells by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution with pH of 4.5, and soaking for 30min;
step four: then adding perlite, vermiculite particles, bagasse and particle slow-release fertilizer, uniformly mixing, and carrying out screw extrusion forming to obtain the culture medium.
Comparative example 1
The comparative example provides a culture substrate which comprises 100 parts by mass of Chinese torreya shells, 10 parts by mass of acetic acid-sodium acetate buffer solution and 0.2 part by mass of granular slow release fertilizer.
Wherein, the acetic acid-sodium acetate buffer solution can adopt the preparation method of the commonly prepared buffer solution in the prior art, namely the preparation method of taking acetic acid and sodium acetate with a certain proportion in a container and adding water to dilute to the preset concentration.
Comparative example 2
The comparative example provides a culture medium which comprises 100 parts of Chinese torreya shells, 20 parts of vermiculite particles, 15 parts of perlite, 10 parts of acetic acid-sodium acetate buffer solution and 0.2 part of particle slow release fertilizer in parts by mass.
Wherein, the acetic acid-sodium acetate buffer solution can adopt the preparation method of the commonly prepared buffer solution in the prior art, namely the preparation method of taking acetic acid and sodium acetate with a certain proportion in a container and adding water to dilute to the preset concentration.
Comparative example 3
The comparative example provides a culture medium which comprises 100 parts by weight of Chinese torreya shells, 4 parts by weight of bagasse, 10 parts by weight of acetic acid-sodium acetate buffer solution and 0.2 part by weight of particle slow-release fertilizer.
Wherein, the acetic acid-sodium acetate buffer solution can adopt the preparation method of the commonly prepared buffer solution in the prior art, namely the preparation method of taking acetic acid and sodium acetate with a certain proportion in a container and adding water to dilute to the preset concentration.
Comparative example 4
The comparative example provides a culture medium which comprises 100 parts by mass of Chinese torreya shells, 20 parts by mass of vermiculite particles, 15 parts by mass of perlite, 4 parts by mass of bagasse, 10 parts by mass of 12% dilute sulfuric acid solution and 0.2 part by mass of particle slow-release fertilizer.
Comparative example 5
The comparative example provides a preparation method of a culture substrate, comprising the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of 2-20mm, crushing the dried Chinese torreya shells by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution with pH of 4.5, and soaking for 30min;
step four: and adding the granular slow release fertilizer, uniformly mixing, and performing screw extrusion forming to obtain the culture medium.
Comparative example 6
The comparative example provides a preparation method of a culture substrate, comprising the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of 2-20mm, crushing the dried Chinese torreya shell raw material by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution with pH of 4.5, and soaking for 30min;
step four: and adding perlite, vermiculite particles and particle slow-release fertilizer, uniformly mixing, and performing screw extrusion forming to obtain the culture medium.
Comparative example 7
The comparative example provides a preparation method of a culture substrate, comprising the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of 2-20mm, crushing the dried Chinese torreya shells by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shells with acetic acid-sodium acetate buffer solution with pH of 4.5, and soaking for 30min;
step four: and adding bagasse and the granular slow-release fertilizer, uniformly mixing, and performing screw extrusion molding to obtain the culture medium.
Comparative example 8
The comparative example provides a preparation method of a culture substrate, comprising the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of 2-20mm, crushing the dried Chinese torreya shells by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: adding 12% dilute sulfuric acid solution into the Chinese torreya shells, mixing until the pH value is adjusted to 4.5, and soaking for 30min;
step four: then adding perlite, vermiculite particles, bagasse and particle slow-release fertilizer, uniformly mixing, and carrying out screw extrusion forming to obtain the culture medium.
Comparative example 9
The comparative example provides a preparation method of a culture substrate, comprising the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of more than 40mm, crushing the dried Chinese torreya shell raw material by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution with pH of 4.5, and soaking for 30min;
step four: then adding perlite, vermiculite particles, bagasse and particle slow-release fertilizer, uniformly mixing, and performing screw extrusion forming to obtain the culture medium.
Comparative example 10
The comparative example provides a preparation method of a culture substrate, comprising the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying until the water content is less than 20%;
step two: selecting Chinese torreya shells with the particle size of less than 1mm, selecting Chinese torreya shells with the particle size of more than 40mm, crushing the dried Chinese torreya shells by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder;
step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution with pH of 4.5, and soaking for 30min;
step four: then adding perlite, vermiculite particles, bagasse and particle slow-release fertilizer, uniformly mixing, and carrying out screw extrusion forming to obtain the culture medium.
Application example 1
A culture substrate 1 was prepared by the preparation method described in example 6 using the culture substrate described in example 3;
a culture substrate 2 was prepared by the preparation method described in comparative example 5 using the culture substrate described in comparative example 1;
a culture substrate 3 was prepared by the preparation method described in comparative example 6 using the culture substrate described in comparative example 2;
a culture substrate 4 was prepared by the preparation method described in comparative example 7 using the culture substrate described in comparative example 3;
a culture substrate 5 was prepared by the preparation method described in comparative example 8 using the culture substrate described in comparative example 4;
a culture substrate 6 was prepared by the preparation method described in comparative example 9 using the culture substrate described in example 3;
a culture substrate 7 was prepared by the preparation method described in comparative example 10 using the culture substrate described in example 3;
the rice seedling raising simulation is realized by sowing the rice on the plastic hard disks, and the culture mediums 1-7 with equal mass are respectively filled into seven plastic hard disks with the same size and are paved. The same batch of rice seeds are equally divided into seven parts, respectively sowed into plastic hard disks filled with culture mediums 1-7, and placed into an illumination incubator for culture under the same culture conditions. After 30 days of sowing, the plant height, above-ground dry weight and underground dry weight of rice were measured and averaged, and the results are shown in the following table:
Figure BDA0003958699820000091
Figure BDA0003958699820000101
and (4) analyzing results: from the above experimental results, it can be seen that the growth momentum of rice in the plastic hard disk using the substrate 1 is the most favorable, and thus the intended object of the present invention is achieved.
The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.

Claims (10)

1. A culture medium comprises Chinese torreya shells, and is characterized in that: also comprises vermiculite particles, perlite, bagasse, buffer solution and particle slow release fertilizer.
2. The culture substrate according to claim 1, wherein: the culture medium comprises 80-120 parts by weight of Chinese torreya shells, 10-30 parts by weight of vermiculite particles, 10-20 parts by weight of perlite, 2-6 parts by weight of bagasse, 8-12 parts by weight of buffer solution and 0.15-0.25 part by weight of particle slow release fertilizer.
3. The culture substrate according to claim 2, wherein: the following steps: the culture medium comprises, by mass, 100 parts of Chinese torreya shells, 20 parts of vermiculite particles, 15 parts of perlite, 4 parts of bagasse, 10 parts of buffer solution and 0.2 part of particle slow-release fertilizer.
4. The culture substrate according to claim 1, wherein: the buffer solution is acetic acid-sodium acetate buffer solution.
5. The culture substrate according to claim 4, wherein: the acetic acid-sodium acetate buffer solution is prepared by the following method: taking 18g of sodium acetate and 9.8ml of glacial acetic acid, and adding water to dilute to 1000ml to obtain an acetic acid-sodium acetate buffer solution.
6. The preparation method of the culture medium is characterized by comprising the following steps:
the method comprises the following steps: collecting Chinese torreya shell raw material and drying;
step two: crushing the dried Chinese torreya shell raw material by using a crusher, extruding by using a screw rod, and screening to obtain Chinese torreya shell powder; (ii) a
Step three: mixing Chinese torreya shell powder with acetic acid-sodium acetate buffer solution, and soaking;
step four: then adding perlite, vermiculite particles, bagasse and particle slow-release fertilizer, uniformly mixing, and performing screw extrusion forming to obtain the culture medium.
7. The method for preparing a culture medium according to claim 6, wherein: after drying in the step one, the water content of the Chinese torreya shell raw material is less than 20%.
8. The method for preparing a culture medium according to claim 6, wherein: the particle size of the Chinese torreya shells selected in the step two is 2-20mm.
9. The method for producing a culture substrate according to claim 6, wherein: the pH value of the acetic acid-sodium acetate buffer solution in the third step is 4-5.
10. The method for producing a culture substrate according to claim 6, wherein: the soaking time in the third step is 20-40min.
CN202211471749.5A 2022-11-23 2022-11-23 Culture medium and preparation method thereof Pending CN115735716A (en)

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