CN115708553A - Composition containing naringin and application of composition in preparation of beverage containing naringin - Google Patents
Composition containing naringin and application of composition in preparation of beverage containing naringin Download PDFInfo
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- CN115708553A CN115708553A CN202211499603.1A CN202211499603A CN115708553A CN 115708553 A CN115708553 A CN 115708553A CN 202211499603 A CN202211499603 A CN 202211499603A CN 115708553 A CN115708553 A CN 115708553A
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- naringin
- organic solvent
- extract
- rhizoma polygonati
- acetone
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- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 title claims abstract description 164
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 title claims abstract description 164
- 229930019673 naringin Natural products 0.000 title claims abstract description 164
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- 239000003960 organic solvent Substances 0.000 claims description 72
- 241000037831 Polygonatum sibiricum Species 0.000 claims description 43
- 239000012046 mixed solvent Substances 0.000 claims description 37
- JYGYEBCBALMPDC-UHFFFAOYSA-N heptane;propan-2-one Chemical compound CC(C)=O.CCCCCCC JYGYEBCBALMPDC-UHFFFAOYSA-N 0.000 claims description 30
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 28
- 239000000741 silica gel Substances 0.000 claims description 28
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- 238000000034 method Methods 0.000 claims description 24
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- 238000001035 drying Methods 0.000 claims description 17
- 239000012535 impurity Substances 0.000 claims description 15
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- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- 239000008280 blood Substances 0.000 abstract description 34
- 210000004369 blood Anatomy 0.000 abstract description 34
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- 240000008866 Ziziphus nummularia Species 0.000 abstract description 15
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- 206010061218 Inflammation Diseases 0.000 abstract description 2
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- 229940079593 drug Drugs 0.000 abstract description 2
- 241000725643 Respiratory syncytial virus Species 0.000 description 34
- 230000001603 reducing effect Effects 0.000 description 21
- 238000010828 elution Methods 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 230000000052 comparative effect Effects 0.000 description 15
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 13
- 238000002156 mixing Methods 0.000 description 13
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 12
- 244000303040 Glycyrrhiza glabra Species 0.000 description 11
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 11
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
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Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a composition containing naringin and application thereof in preparing a beverage containing naringin. The composition containing naringin comprises naringin and a rhizoma polygonati and spina date seed extract; wherein the weight ratio of the naringin to the sealwort and spina date seed extract is 1-2; the rhizoma polygonati and semen ziziphi spinosae extract is obtained by extracting rhizoma polygonati and semen ziziphi spinosae as raw materials. The composition containing the naringin is formed by combining the naringin and the extract of the wild jujube seeds of the sealwort; therefore, products (such as beverages, foods, medicines or health products) prepared by taking the composition containing the naringin as an effective component not only have the functions of anti-inflammation, antivirus, anticancer, antimutation, antianaphylaxis, antiulcer, analgesia, blood pressure reduction and the like of the naringin; also has blood sugar lowering effect.
Description
Technical Field
The invention relates to the technical field of natural products and foods, in particular to a composition containing naringin and application thereof in preparing a beverage containing naringin.
Background
Naringin, is a flavonoid compound; has antiinflammatory, antiviral, anticancer, antimutagenic, antiallergic, antiulcer, analgesic, blood pressure lowering, blood cholesterol reducing, thrombosis reducing, local microcirculation improving and nutrition supply improving effects, and can be used for preventing and treating cardiovascular disease and cerebrovascular disease.
Although naringin has a number of pharmacological activities; however, when the compound is used as a single active ingredient, the effects of various aspects still need to be improved; for example, its effects in lowering blood sugar and resisting virus are yet to be enhanced.
Chinese patent CN 113133512A discloses a beverage containing naringin and a preparation method thereof, which is prepared by taking the naringin as an active additive component. But only the naringin is taken as an active ingredient, so that the prepared beverage containing the naringin has a certain health-care effect; however, the effects of the composition on the aspects of assisting blood sugar reduction and resisting viruses are still to be strengthened.
Disclosure of Invention
In order to overcome at least one of the technical problems of the prior art, the present invention firstly provides a composition containing naringin. The composition containing the naringin is prepared by combining the naringin and the extracts of the sealwort and the spina date seeds, and has a good effect of reducing blood sugar.
The invention aims to solve the technical problems and is realized by the following technical scheme:
a composition containing naringin comprises naringin and rhizoma Polygonati and semen Ziziphi Spinosae extract;
wherein the weight ratio of the naringin to the polygonatum sibiricum spine date seed extract is 1-2;
the rhizoma polygonati and spina date seed extract is obtained by extracting rhizoma polygonati and spina date seed as raw materials.
The inventor finds that the composition obtained by combining naringin and the polygonatum sibiricum spine date seed extract has a good blood sugar reducing effect.
In addition, the inventor also surprisingly discovers in experimental screening that the composition obtained by combining naringin and the polygonatum sibiricum spine date seed extract also has the effect of resisting respiratory syncytial virus.
Preferably, the polygonatum sibiricum spine date seed extract is prepared by the following method:
the preparation method comprises the steps of extracting rhizoma polygonati and spina date seeds by using an organic solvent to obtain an organic solvent extracting solution of the rhizoma polygonati and the spina date seeds, removing the organic solvent from the organic solvent extracting solution of the rhizoma polygonati and the spina date seeds, and drying to obtain an organic solvent extract of the rhizoma polygonati and the spina date seeds.
Preferably, the organic solvent is a mixed organic solvent consisting of isopropanol and acetone in a volume ratio of 3-5.
Preferably, the weight ratio of the sealwort to the spina date seed is 1-3.
The inventor needs to emphasize that the selection of the extraction solvent of the sealwort and the spina date seed plays an important role in determining whether the extracted sealwort and spina date seed extract and naringin have good blood sugar reducing and respiratory syncytial virus resisting effects after being combined. The inventor surprisingly finds out in a large number of experiments that the composition obtained by combining the polygonatum sibiricum spine date seed extract obtained by extracting with the mixed organic solvent consisting of isopropanol and acetone and naringin has the effects of reducing blood sugar and resisting respiratory syncytial virus which are obviously higher than the composition obtained by combining the polygonatum sibiricum spine date seed extract obtained by extracting with other organic solvents and the naringin.
Preferably, the extraction is performed by heating and refluxing for 1-2 h at 50-55 ℃.
Preferably, the weight consumption of the organic solvent is 5-10 times of the total weight of the rhizoma polygonati and the spina date seed;
most preferably, the weight of the organic solvent is 8 times of the total weight of the sealwort and the spina date seed.
Preferably, the polygonatum sibiricum spine date seed extract is prepared by the following method:
extracting rhizoma Polygonati and semen Ziziphi Spinosae with organic solvent to obtain rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extractive solution, removing organic solvent from rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extractive solution, and drying to obtain rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extract;
further feeding the extract of the polygonatum sibiricum and spina date seed organic solvent into a silica gel column, and eluting and removing impurities by using an n-heptane acetone mixed solvent with the acetone volume fraction of 5-8%; then eluting with a mixed solvent of n-heptane acetone with acetone volume fraction of 17-20%, collecting the fraction eluted by the mixed solvent of n-heptane acetone with acetone volume fraction of 17-20%, removing the organic solvent and drying to obtain the polygonatum sibiricum spine date seed extract.
The n-heptane acetone mixed solvent in which the acetone volume fraction is 5 to 8% means that the acetone volume fraction in the n-heptane acetone mixed solvent composed of n-heptane and acetone is 5 to 8%.
The n-heptane-acetone mixed solvent in which the acetone volume fraction is 17 to 20% means that the acetone volume fraction is 17 to 20% in the n-heptane-acetone mixed solvent composed of n-heptane and acetone.
In order to further improve the hypoglycemic and respiratory syncytial virus resistant effects of the composition containing the naringin; the inventor further refines the effective part from the extract of the polygonatum sibiricum spine date seed organic solvent by silica gel column chromatography; the inventor surprisingly discovers in a large number of experiments that in the elution process of a silica gel column, the composition obtained by combining the polygonatum sibiricum seed and wild jujube seed extract and naringin prepared by the elution condition (firstly eluting with an n-heptane acetone mixed solvent with the acetone volume fraction of 5-8% and removing impurities, and then eluting with an n-heptane acetone mixed solvent with the acetone volume fraction of 17-20%) is further greatly improved in the effects of reducing blood sugar and resisting respiratory syncytial virus compared with the composition obtained by combining the polygonatum sibiricum seed extract and naringin extracted with a mixed organic solvent consisting of isopropanol and acetone; has excellent effects of reducing blood sugar and resisting respiratory syncytial virus.
The inventor needs to emphasize that the elution condition of the silica gel column is crucial, the elution condition of the silica gel column is different, and the composition and the content of the active ingredients in the prepared polygonatum sibiricum spine date seed extract are different; if the elution condition of the silica gel column is not properly selected, the content of effective components with the effects of reducing blood sugar and resisting respiratory syncytial virus in the prepared polygonatum sibiricum spine date seed extract is possibly reduced; this not only fails to further increase the hypoglycemic and anti-respiratory syncytial virus effects, but may even decrease them.
The composition containing naringin has more remarkable effects of reducing blood sugar and resisting respiratory syncytial virus; therefore, when the equivalent effect of reducing blood sugar and resisting the respiratory syncytial virus is required, the effect can be realized by adding smaller amount of the composition. If the composition is added with the same dosage, the composition has stronger effects of reducing blood sugar and resisting respiratory syncytial virus.
Preferably, the composition containing naringin also comprises licorice extract;
wherein the weight ratio of the naringin to the extracts of the polygonatum sibiricum and the wild jujube and the liquorice is 1-2.
The licorice extract is prepared by the following method:
heating and refluxing Glycyrrhrizae radix with water to obtain extractive solution, concentrating and drying to obtain Glycyrrhrizae radix water extract;
and (2) putting the licorice aqueous extract into a macroporous resin column, eluting with water to remove impurities, eluting with an ethanol aqueous solution with the volume fraction of 4-6%, collecting the fraction eluted with the ethanol aqueous solution with the volume fraction of 4-6%, concentrating and drying to obtain the licorice extract.
Preferably, the extraction is performed by heating and refluxing for 1-2 h at 90-100 ℃.
Preferably, the weight consumption of the organic solvent is 8-15 times of the total weight of the rhizoma polygonati and the spina date seeds;
most preferably, the weight amount of the organic solvent is 12 times of the total weight of the rhizoma polygonati and the spina date seed.
The inventor surprisingly discovers in research that after the liquorice extract prepared by the method is mixed with the naringin and the sealwort and spina date seed extract, the mixture can promote the dissolution of the naringin and the sealwort and spina date seed extract in water and improve the dissolving capacity of the composition containing the naringin in water.
The invention also provides application of the composition containing naringin in preparing products with hypoglycemic and/or antiviral effects.
Preferably, the virus is respiratory syncytial virus.
The product comprises medicines, health products or foods.
The invention also provides application of the composition containing naringin in preparing a beverage containing naringin.
Preferably, the content of the composition containing naringin in 1000mL of the beverage containing naringin is 0.5 to 1g.
Preferably, the beverage containing naringin also contains flavoring agent.
Preferably, the beverage containing naringin also contains a preservative.
Has the advantages that: the invention provides a brand-new composition containing naringin. The composition containing naringin is prepared by combining naringin and extracts of rhizoma polygonati and spina date seeds; therefore, the product (such as beverage, food, medicine or health product) prepared by taking the composition containing the naringin as the active ingredient not only has the functions of anti-inflammation, antivirus, anticancer, antimutation, antianaphylaxis, antiulcer, analgesia, blood pressure reduction and the like of the naringin; simultaneously has the effects of reducing blood sugar and resisting respiratory syncytial virus; when products with the same effects of reducing blood sugar and resisting respiratory syncytial virus are required to be obtained, the effects can be realized by adding a smaller amount of the composition; if the composition with the same dosage is added, the composition has stronger effects of reducing blood sugar and resisting respiratory syncytial virus.
Detailed Description
The present invention will be further explained with reference to specific examples, which are not intended to limit the present invention in any way.
Example 1 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 2;
the sealwort and wild jujube seed extract is prepared by the following method:
taking rhizoma polygonati and spina date seeds in a weight ratio of 1;
the organic solvent is a mixed organic solvent consisting of isopropanol and acetone in a volume ratio of 4.
Example 2 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 2;
the sealwort and spina date seed extract is prepared by the following method:
taking rhizoma polygonati and spina date seeds in a weight ratio of 1;
loading the rhizoma polygonati and spina date seed organic solvent extract to a silica gel column filled with 200-300 meshes of silica gel with the weight 30 times that of the rhizoma polygonati and spina date seed organic solvent extract, and eluting and removing impurities by using a 3BV n-heptane-acetone mixed solvent with the acetone volume fraction accounting for 7 percent at the flow rate of 5 BV/h; eluting with 5BV of n-heptane-acetone mixed solvent with acetone volume fraction of 19%, collecting the fraction eluted from the n-heptane-acetone mixed solvent with acetone volume fraction of 19%, concentrating to remove organic solvent, and drying to obtain the rhizoma Polygonati and semen Ziziphi Spinosae extract.
Example 3 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 1;
the sealwort and spina date seed extract is prepared by the following method:
taking rhizoma polygonati and spina date seeds in a weight ratio of 3;
loading the rhizoma polygonati and spina date seed organic solvent extract to a silica gel column filled with 200-300 meshes of silica gel with the weight 30 times that of the rhizoma polygonati and spina date seed organic solvent extract; eluting with 3BV of n-heptane acetone mixed solvent with acetone volume fraction of 5% at a flow rate of 5BV/h to remove impurities; eluting with 5BV of n-heptane-acetone mixed solvent with acetone volume fraction of 17%, collecting the fraction eluted from the n-heptane-acetone mixed solvent with acetone volume fraction of 17%, concentrating to remove organic solvent, and drying to obtain the rhizoma Polygonati and semen Ziziphi Spinosae extract.
Example 4 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 1;
the sealwort and wild jujube seed extract is prepared by the following method:
taking the sealwort and the spina date seed in a weight ratio of 1 to 3, heating and refluxing for 2 hours at 50 ℃ by using an organic solvent which is 10 times of the total weight of the sealwort and the spina date seed to obtain an organic solvent extracting solution of the sealwort and the spina date seed, concentrating the organic solvent extracting solution of the sealwort and the spina date seed to remove the organic solvent, and drying to obtain an organic solvent extract of the sealwort and the spina date seed;
loading the rhizoma polygonati and spina date seed organic solvent extract to a silica gel column filled with 200-300 meshes of silica gel with the weight 30 times that of the rhizoma polygonati and spina date seed organic solvent extract; eluting with 3BV of n-heptane acetone mixed solvent with acetone volume fraction of 8% at a flow rate of 5BV/h to remove impurities; eluting with 5BV of n-heptane-acetone mixed solvent with acetone volume fraction of 20%, collecting the fraction eluted from the n-heptane-acetone mixed solvent with acetone volume fraction of 20%, concentrating to remove organic solvent, and drying to obtain the extract.
Example 5 composition containing Naringin
The composition containing the naringin is formed by mixing the naringin, the sealwort and wild jujube seed extract and the liquorice extract according to the weight ratio of 2;
the preparation method of the polygonatum sibiricum spine date seed extract is the same as that in the embodiment 1;
the licorice extract is prepared by the following method:
taking liquorice, then carrying out heating reflux extraction for 1h at 100 ℃ by using water with the weight being 12 times that of the liquorice, obtaining an extracting solution after the extraction is finished, and concentrating and drying the extracting solution to obtain a liquorice water extract;
loading a liquorice aqueous extract on a macroporous resin column filled with D101 type macroporous resin which is 30 times the weight of the liquorice aqueous extract, eluting with 1BV of water at the flow rate of 2BV/h to remove impurities, eluting with 3BV of 5% ethanol aqueous solution, collecting fractions eluted by the 5% ethanol aqueous solution, concentrating and drying to obtain the liquorice extract.
Example 6 Naringin-containing composition
The composition containing the naringin is formed by mixing the naringin, the sealwort and wild jujube seed extract and the liquorice extract according to the weight ratio of 2;
the preparation method of the polygonatum sibiricum spine date seed extract is the same as that in the embodiment 2;
the preparation method of the licorice extract is the same as that of example 5.
Comparative example 1 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 2;
the sealwort and spina date seed extract is prepared by the following method:
taking rhizoma polygonati and spina date seeds in a weight ratio of 1;
the organic solvent is ethanol with the volume fraction of 95 percent.
Comparative example 2 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 2;
the sealwort and wild jujube seed extract is prepared by the following method:
taking the sealwort and the spina date seed in a weight ratio of 1.
Comparative example 3 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 2;
the sealwort and wild jujube seed extract is prepared by the following method:
taking rhizoma polygonati and spina date seeds in a weight ratio of 1;
the organic solvent is isopropanol.
Comparative example 4 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 2;
the sealwort and wild jujube seed extract is prepared by the following method:
taking the sealwort and the spina date seed in a weight ratio of 1;
the organic solvent is acetone.
Comparative example 5 composition containing naringin
The composition containing naringin is prepared by mixing naringin and rhizoma polygonati and semen ziziphi spinosae extracts in a weight ratio of 2;
the sealwort and wild jujube seed extract is prepared by the following method:
taking the sealwort and the spina date seed in a weight ratio of 1;
loading the organic solvent extract of rhizoma Polygonati and semen Ziziphi Spinosae onto silica gel column filled with 200-300 mesh silica gel 30 times the weight of the organic solvent extract of rhizoma Polygonati and semen Ziziphi Spinosae, eluting with 3BV mixed solvent of petroleum ether and acetone with acetone volume fraction 7% at flow rate of 5BV/h to remove impurities; eluting with 5BV of mixed solvent of petroleum ether and acetone with acetone volume fraction of 19%, collecting the fraction eluted from mixed solvent of petroleum ether and acetone with acetone volume fraction of 19%, concentrating to remove organic solvent, and drying to obtain the extract.
Comparative example 6 composition containing naringin
The composition containing naringin is prepared by mixing naringin and sealwort and spina date seed extracts in a weight ratio of 2;
the sealwort and wild jujube seed extract is prepared by the following method:
taking the sealwort and the spina date seed in a weight ratio of 1;
loading the rhizoma polygonati and spina date seed organic solvent extract to a silica gel column filled with 200-300 meshes of silica gel with the weight 30 times that of the rhizoma polygonati and spina date seed organic solvent extract, and eluting with 3BV of n-heptane at the flow rate of 5BV/h to remove impurities; eluting with 5BV of n-heptane-acetone mixed solvent with acetone volume fraction of 10%, collecting the fraction eluted from the n-heptane-acetone mixed solvent with acetone volume fraction of 10%, concentrating to remove organic solvent, and drying to obtain the extract.
Comparative example 7 composition containing naringin
The composition containing naringin is prepared by mixing naringin and sealwort and spina date seed extracts in a weight ratio of 2;
the sealwort and wild jujube seed extract is prepared by the following method:
taking the sealwort and the spina date seed in a weight ratio of 1;
loading the rhizoma polygonati and spina date seed organic solvent extract to a silica gel column filled with 200-300 meshes of silica gel with the weight 30 times that of the rhizoma polygonati and spina date seed organic solvent extract, eluting and removing impurities by using a 3BV n-heptane-acetone mixed solvent with the acetone volume fraction accounting for 20 percent at the flow rate of 5 BV/h; eluting with 5BV of n-heptane-acetone mixed solvent with acetone volume fraction of 30%, collecting the fraction eluted from the n-heptane-acetone mixed solvent with acetone volume fraction of 30%, concentrating to remove organic solvent, and drying to obtain the rhizoma Polygonati and semen Ziziphi Spinosae extract.
Experimental example 1 blood sugar lowering experiment of composition containing naringin
The diabetes model mice with blood sugar value above 10mmol/L are randomly grouped into model groups and experimental groups, and normal mice are selected as normal group controls, wherein each group comprises 10 mice. The experimental group is administered by gavage for 1 time every day, and the administration dose is 100 mg/(kg. D); the model group and the normal group were perfused with the same amount of physiological saline. Continuously gavage for 6 weeks, collecting blood and measuring fasting blood glucose value; rats are fasted for 12h before blood drawing; specific results are shown in table 1.
The samples for intragastric administration in the experimental group of this example were: naringin-containing compositions prepared according to examples 1 to 2 and ratios 1 to 7. The test results are shown in Table 1.
TABLE 1 Effect of naringin-containing compositions on fasting plasma glucose in diabetic mice
As can be seen from the experimental data in table 1, the composition containing naringin prepared in example 1 has significantly lower fasting blood glucose to mice 6 weeks after gavage administration than the model group; this indicates that: the composition obtained by combining naringin and the polygonatum sibiricum and spina date seed extracts prepared by the method has a good blood sugar reducing effect.
As can be seen from the experimental data in table 1, compared with the model group, the decrease degree of the fasting blood glucose value of the mice after the composition containing naringin prepared in comparative examples 1 to 4 is administrated by gastric lavage for 6 weeks is significantly smaller than that of example 1; this indicates that: the selection of the extraction solvent of the sealwort and the spina date seed plays an important role in determining whether the extracted sealwort and spina date seed extract and naringin have a good blood sugar reducing effect after being combined. The composition obtained by combining the polygonatum sibiricum spine date seed extract obtained by extracting with a mixed organic solvent consisting of isopropanol and acetone and naringin has a blood sugar lowering effect which is remarkably higher than that of the composition obtained by combining the polygonatum sibiricum spine date seed extract obtained by extracting with other organic solvents and naringin.
As can be seen from the experimental data in Table 1, the fasting blood glucose of the mouse 6 weeks after the administration by gavage of the composition containing naringin prepared in example 1 is significantly lower than that of the composition containing naringin prepared in comparative examples 3 and 4. This indicates that: the sealwort and spina date seed extract is extracted by using a mixed organic solvent consisting of isopropanol and acetone, and can synergistically improve the blood sugar reducing effect of a composition obtained by combining the sealwort and spina date seed extract and naringin.
As can be seen from the experimental data in table 1, the fasting blood glucose of the composition containing naringin prepared in example 2 after 6 weeks of administration by gavage is further greatly smaller than that of example 1; this indicates that: in the elution process of the silica gel column, the composition obtained by combining the polygonatum sibiricum spine date seed extract and the naringin is prepared by adopting the elution conditions (firstly, the normal heptane acetone mixed solvent with the acetone volume fraction of 5-8% is used for eluting and removing impurities, and then, the normal heptane acetone mixed solvent with the acetone volume fraction of 17-20% is used for eluting), and compared with the composition obtained by combining the polygonatum sibiricum spine date seed organic solvent extract and the naringin in the embodiment 1, the hypoglycemic effect of the composition is further and greatly improved; has excellent hypoglycemic effect.
As can be seen from the experimental data in table 1, the compositions containing naringin prepared in comparative examples 5 and 6 have a much smaller reduction in fasting plasma glucose in mice 6 weeks after gavage than that in example 1, and the reduction is much smaller than that in example 2; the composition containing naringin prepared in comparative example 7 has fasting blood glucose to mice after 6 weeks of gavage administration even higher than that of example 1; this indicates that: the elution condition of the silica gel column is crucial, the elution conditions of the silica gel column are different, and the composition and the content of the effective components in the prepared rhizoma polygonati and spina date seed extracts are different; if the elution condition of the silica gel column is not properly selected, the content of the effective components with the blood sugar reducing effect in the prepared polygonatum sibiricum spine date seed extract is possibly reduced; this not only does not further improve the hypoglycemic effect, but may even reduce it. Therefore, the hypoglycemic effect of the composition obtained by combining the polygonatum sibiricum and spina date seed extract and the naringin can be greatly improved only by using the elution conditions (firstly, using the n-heptane-acetone mixed solvent with the acetone volume fraction of 5-8% to elute and remove impurities and then using the n-heptane-acetone mixed solvent with the acetone volume fraction of 17-20% to elute) provided by the invention, so that the excellent hypoglycemic effect is achieved; the composition obtained by combining the polygonatum sibiricum spine date seed extract and the naringin prepared under other elution conditions cannot greatly improve the blood sugar reducing effect and does not have very excellent blood sugar reducing effect.
Experimental example 2 anti-respiratory syncytial Virus Effect of the composition containing Naringin
2X 10 inoculations per 1mL of 24-well cell culture plates 5 cell/mL HEp-2 cells; then cultured in an incubator (37 ℃,5% CO) 2 ) To a monolayer of growing cells; then 100 mu L of respiratory syncytial virus suspension 100PFU/mL and sample solution to be detected 100 mu L with different concentrations are added into each hole of the experimental group; in addition, a virus control group and a cell control group are also arranged; then, each group was cultured in an incubator (37 ℃,5% CO) 2 ) 2h; after the culture is finished, the virus liquid is sucked and discarded, PBS is used for washing for 3 times, 0.5mL of agarose gel covering liquid is added into each hole for covering, after the agarose gel is solidified, 0.5mL of sample solution to be detected with corresponding concentration is added into no hole of the experimental group, and maintenance liquid with the same volume is respectively added into each hole of the virus control group and the cell control group; then cultured in an incubator (37 ℃,5% CO) 2 ) 4-5 d, after the culture is finished, adding formaldehyde (10%) to fix for one night, then removing agarose gel, dyeing for 40min by using 1% crystal violet, washing the culture plate 2 by using water after the dyeing is finished, and counting the number of plaques after the culture plate is dried; further, the plaque inhibition rate of the sample was calculated from the number of plaques, and then the median inhibitory concentration IC50 was calculated from the plaque inhibition rate.
The IC50 values of the experimental groups are shown in Table 2, and the samples tested in the experimental groups are the compositions containing naringin prepared according to examples 1-2 and proportions 1-7 respectively.
TABLE 1 naringin-containing composition respiratory syncytial virus resistance test results
As can be seen from the experimental data in Table 1, the naringin-containing composition prepared in example 1 has an IC50 value for respiratory syncytial virus of 15.5. Mu.g/mL; this indicates that: the composition obtained by combining naringin and the polygonatum sibiricum spine date seed extract prepared by the method has better effect of resisting respiratory syncytial virus.
As can be seen from the experimental data in Table 1, the IC50 values of the compositions containing naringin prepared in comparative examples 1 to 4 on the respiratory syncytial virus are greatly higher than that of example 1; this indicates that: the selection of the extraction solvent of the sealwort and the spina date seed plays an important role in determining whether the obtained sealwort and spina date seed extract and naringin have better respiratory syncytial virus resistance after being combined. The composition obtained by combining the polygonatum sibiricum spine date seed extract obtained by extracting with a mixed organic solvent consisting of isopropanol and acetone and naringin has the effect of resisting respiratory syncytial virus which is obviously higher than that of the composition obtained by combining the polygonatum sibiricum spine date seed extract obtained by extracting with other organic solvents and the naringin.
As can be seen from the experimental data in table 1, the naringin-containing composition prepared in example 1 also has a significantly lower IC50 value for respiratory syncytial virus than the naringin-containing compositions prepared in comparative examples 3 and 4. This indicates that: the composition is prepared by extracting the polygonatum sibiricum spine date seed extract by using a mixed organic solvent consisting of isopropanol and acetone, and can synergistically improve the respiratory syncytial virus resistance of the composition prepared by combining the polygonatum sibiricum spine date seed extract and naringin.
As can be seen from the experimental data in table 1, the IC50 value of the composition containing naringin prepared in example 2 against respiratory syncytial virus is much smaller than that of example 1; this indicates that: the composition obtained by combining the polygonatum sibiricum spine date seed extract and the naringin prepared by the silica gel column elution condition (firstly eluting with an n-heptane acetone mixed solvent with the acetone volume fraction of 5-8% to remove impurities and then eluting with an n-heptane acetone mixed solvent with the acetone volume fraction of 17-20%) is further adopted, and compared with the composition obtained by combining the polygonatum sibiricum spine date seed organic solvent extract and the naringin in the embodiment 1, the respiratory syncytial virus resistance of the composition is further and greatly improved; has excellent effect of resisting respiratory syncytial virus.
As can be seen from the experimental data in table 1, the IC50 values of the compositions containing naringin prepared in comparative examples 5 and 6 for respiratory syncytial virus are not reduced much compared with example 1, and the reduction is much smaller than that of example 2; comparative example 7 the naringin-containing composition prepared has an IC50 value for respiratory syncytial virus even higher than that of example 1; this indicates that: the elution condition of the silica gel column is crucial, the elution conditions of the silica gel column are different, and the composition and the content of the effective components in the prepared polygonatum sibiricum spine date seed extract are different; if the elution condition of the silica gel column is not properly selected, the content of effective components with the effect of resisting the respiratory syncytial virus in the prepared polygonatum sibiricum spine date seed extract is possibly reduced; this not only does not further improve the effect against respiratory syncytial virus, but may even reduce it. Therefore, the composition obtained by combining the polygonatum sibiricum wild jujube extract and the naringin, which is prepared by the silica gel column elution condition (firstly, the normal heptane acetone mixed solvent with the acetone volume fraction of 5-8% is used for elution and impurity removal, and then, the normal heptane acetone mixed solvent with the acetone volume fraction of 17-20% is used for elution), can greatly improve the effect of resisting the respiratory syncytial virus, and has very excellent effect of resisting the respiratory syncytial virus; the composition prepared by combining the polygonatum sibiricum spine date seed extract and the naringin under other silica gel column elution conditions cannot greatly improve the respiratory syncytial virus resistance, and does not have very excellent respiratory syncytial virus resistance.
Experimental example 3 solubility experiment
1g of the composition containing naringin prepared in example 1 and examples 5 and 6 was added to a beaker, followed by adding 200mL of water, stirring uniformly, and after standing for 24 hours, observing whether a precipitate was generated; the results are shown in Table 3.
TABLE 3 solubility test results for compositions containing naringin
As can be seen from the experimental results in table 3, the composition containing naringin, which is composed of naringin and the extract of the wild jujube seed of polygonatum prepared in example 1, has poor solubility in water, and obvious precipitation appears after 24 hours of standing. In examples 5 and 6, a composition containing naringin, which is composed of the licorice extract prepared by the method of the invention, is added into the naringin and the polygonatum sibiricum spine date seed extract, and no precipitate is generated after the composition is placed for 24 hours; this indicates that: after the liquorice extract prepared by the method is mixed with the naringin and the sealwort and spina date seed extracts, the dissolving capacity of the naringin and the sealwort and spina date seed extracts in water can be improved.
Example 7 preparation of a Naringin-containing beverage
1g of the composition containing the naringin prepared in the embodiment 1 and 0.2g of flavoring agent are added into 1000mL of purified water, the mixture is uniformly stirred, and then the beverage containing the naringin is obtained after sterilization.
EXAMPLE 8 preparation of a drink containing Naringin
And (2) adding 0.5g of the composition containing the naringin prepared in the embodiment 2 and 0.2g of a flavoring agent into 1000mL of purified water, uniformly stirring, and sterilizing to obtain the beverage containing the naringin.
Example 9 preparation of a Naringin-containing beverage
1g of the composition containing the naringin prepared in the embodiment 5 and 0.1g of flavoring agent are added into 1000mL of purified water, the mixture is uniformly stirred, and then the beverage containing the naringin is obtained after sterilization.
Example 10 preparation of a Naringin-containing beverage
1g of the composition containing the naringin prepared in the embodiment 6 and 0.1g of flavoring agent are added into 1000mL of purified water, the mixture is uniformly stirred, and then the beverage containing the naringin is obtained after sterilization.
Claims (10)
1. A composition containing naringin is characterized by comprising naringin and extracts of rhizoma polygonati and spina date seeds;
wherein the weight ratio of the naringin to the polygonatum sibiricum spine date seed extract is 1-2;
the rhizoma polygonati and semen ziziphi spinosae extract is obtained by extracting rhizoma polygonati and semen ziziphi spinosae as raw materials.
2. The naringin-containing composition of claim 1, wherein said polygonatum sibiricum licorice extract is prepared by the following method:
extracting rhizoma Polygonati and semen Ziziphi Spinosae with organic solvent to obtain rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extractive solution, removing organic solvent from rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extractive solution, and drying to obtain rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extract.
3. The composition containing naringin according to claim 2, wherein the organic solvent is a mixed organic solvent consisting of isopropanol and acetone in a volume ratio of 3 to 5.
4. The composition containing naringin according to claim 2, wherein the weight ratio of polygonatum sibiricum to spine date seed is 1-3.
5. The naringin-containing composition according to claim 2, wherein the extract of Polygonatum sibiricum and Zizyphi spinosa L is prepared by the following method:
extracting rhizoma Polygonati and semen Ziziphi Spinosae with organic solvent to obtain rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extractive solution, removing organic solvent from rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extractive solution, and drying to obtain rhizoma Polygonati and semen Ziziphi Spinosae organic solvent extract;
further feeding the extract of the polygonatum sibiricum and spina date seed organic solvent into a silica gel column, and eluting and removing impurities by using an n-heptane acetone mixed solvent with the acetone volume fraction of 5-8%; then eluting with a mixed solvent of n-heptane acetone with acetone volume fraction of 17-20%, collecting the fraction eluted by the mixed solvent of n-heptane acetone with acetone volume fraction of 17-20%, removing the organic solvent and drying to obtain the polygonatum sibiricum spine date seed extract.
6. Use of the composition containing naringin as claimed in any one of claims 1 to 5 in the preparation of products with hypoglycemic and/or antiviral effects.
7. Use of the naringin-containing composition as claimed in any one of claims 1 to 5 in the preparation of a naringin-containing beverage.
8. The use as claimed in claim 7, wherein the naringin-containing composition is present in an amount of 0.5 to 1g in 1000mL of the naringin-containing beverage.
9. The use of claim 7, wherein the naringin-containing beverage further comprises a flavoring agent.
10. The use according to claim 7, wherein the naringin-containing beverage further comprises a preservative.
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