CN115707952A - Sensor capable of measuring viscosity and concentration of glucose solution based on photoelectric response and biosensing technology - Google Patents

Abstract

The invention provides a sensor capable of measuring the viscosity and concentration of a glucose solution based on photoelectric response and biosensing technology. Specifically, the invention provides a sensor for measuring the viscosity and concentration of glucose solution, comprising: the sensor can complete the measurement of the viscosity and the concentration of a glucose solution in the same system, namely the viscosity of glucose is measured firstly, then the concentration of the glucose is measured, and the traditional measurement of the concentration and the viscosity of the glucose needs to be completed independently in different systems.

Description

Sensor capable of measuring viscosity and concentration of glucose solution based on photoelectric response and biosensing technology

Technical Field

The invention relates to the technical field of medical sensors, in particular to a sensor capable of measuring the viscosity and concentration of a glucose solution based on photoelectric response and biosensing technology.

Background

Viscosity is a physicochemical property of a substance, is a measurement of fluid viscosity, is a representation of fluid flow force to internal friction phenomenon, has important application in many fields, and is an important judgment basis for reflecting physiological conditions of body fluid viscosity measurement values such as sweat, serum and the like in clinical detection. For example, cardiovascular diseases such as myocardial infarction, cerebral infarction, etc., are associated with abnormalities in many blood parameter indices. The measurement of the viscosity of serum is a very simple and effective method, the increase of the viscosity of serum represents that blood may contain more blood sugar and blood fat, and the increase of the viscosity of blood obviously increases the resistance of blood flow, thereby affecting the normal perfusion of microcirculation. In addition, viscosity is also one of the important parameters of some liquid medicines, which reflects the concentration and quality of the medicine.

Viscosity measurements are currently mainly: a rotation method, a capillary method, a vibration method, and the like. At present, viscosity measurement generally cannot realize simple measurement, moving parts are needed, and specific requirements such as application range, measurement precision, reaction speed, economy, operation difficulty and the like cannot be met. For human body fluid, the traditional viscosity detection instrument has the disadvantages of large equipment size, large volume measurement, complex operation, high requirement on operators, high price, incapability of realizing disposable use and difficult clinical application. The current medical sensors mainly focus on detecting the concentration of relevant substances in body fluid, and the sensors for measuring the viscosity of the body fluid are relatively few, and the viscosity and the concentration of a solution can not be measured simultaneously through a small amount of body fluid.

The biosensor is a device or apparatus that selectively recognizes a specific substance to be measured by using an immobilized biological substance as a molecular recognition element, and is a device or apparatus that is sensitive to the substance to be measured and converts the concentration thereof into an electrical signal for detection, and the biosensor can selectively recognize glucose molecules and measure the concentration by designing a suitable biosensor structure. The existing sensor is difficult to simultaneously measure glucose viscosity parameters and concentration parameters in the same detection system.

Therefore, there is a strong need in the art to develop sensors that perform viscosity parameter and concentration measurements on glucose solutions in the same system.

Disclosure of Invention

The invention aims to provide a sensor for detecting a viscosity parameter and a concentration parameter in the same glucose solution system.

In a first aspect of the present invention, there is provided a sensor for measuring viscosity of a glucose solution and concentration thereof, comprising: a light source module, a reaction module, a glucose viscosity response module, a glucose concentration response module and a response measurement and analysis module, wherein,

the light source module comprises an excitation light source, an optical fiber, an optical lens, a shutter and a timer; the reaction module comprises a substrate and a bottomless container;

the glucose viscosity response module comprises a first electrode, a second electrode and a sensitive membrane element; the glucose concentration response module comprises a third electrode, a fourth electrode and a reference electrode;

the response measuring and analyzing module comprises a measuring interface, an ammeter and a device for data recording and analyzing;

the first electrode, the second electrode, the third electrode, the fourth electrode and the reference electrode are arranged in the reaction module in parallel, the sensitive membrane element is positioned on the first electrode, the light source module is positioned right above the first electrode, and the response measurement and analysis module is respectively connected with the glucose viscosity response module and the glucose concentration response module.

In another preferred example, the glucose viscosity response module and the glucose concentration response module are not connected with each other.

In another preferred embodiment, the sensitive membrane element is an organic semiconductor material, has good stability to water, and is fixed to the first electrode by one or more methods selected from the group consisting of: solution drop coating, physical adsorption, chemical substance fixation, and the like.

In another preferred example, the excitation light source in the light source module emits light with a specific wavelength, and the wavelength of the light belongs to the absorption wavelength of the sensitive film element.

In another preferred embodiment, the substrate comprises a substrate of one or more materials selected from the group consisting of: silicon dioxide, PET or other substrates made of other organic or inorganic materials that are chemically inert, do not affect the electrode response, and have good insulating properties.

In another preferred embodiment, the first electrode comprises an electrode of one or more materials selected from the group consisting of: gold or other materials which do not influence photoelectric response, have stable chemical properties and good conductive function.

In another preferred embodiment, the second electrode comprises an electrode of one or more materials selected from the group consisting of: aluminum, platinum, or other materials that are chemically inert, electrically conductive, and stable, and do not interfere with the reactions at the other electrodes.

In another preferred example, the third electrode comprises a platinum-glucose oxidase-gold thin film other electrode.

In another preferred embodiment, the fourth electrode includes platinum or other electrode made of a material that is inert in chemical properties, good in conductivity and stability, and does not affect the reaction on the other electrode, as the counter electrode of the third electrode.

In another preferred embodiment, the reference electrode comprises an electrode made of a material with electrode potential stability, reproducibility and single reversible reaction, such as a silver-plated/silver chloride electrode or a mercury/mercury chloride electrode.

In another preferred example, the sensitive membrane element comprises a P3HT: PCBM blended membrane or a sensitive membrane made of other organic semiconductor materials which have good photosensitive characteristics and photocurrent response and are easy to form a membrane.

In another preferred example, the device for data recording and analysis includes a computer, a mobile phone and other devices which can be used for data signal processing.

In another preferred example, in the light source module, the light emitted by the excitation light source is coupled to an optical lens through an optical fiber, and the optical lens has a focusing function, and can focus the light emitted by the excitation light source into a collimated light beam, and includes an LED light source or a laser light source.

In another preferred embodiment, the reaction module is used for containing the glucose liquid to be measured, is a place for completing the measurement of the glucose concentration and the viscosity, is a place for realizing the measurement of the glucose concentration through an enzymatic reaction, ensures that the glucose concentration response module and the glucose viscosity response module are fully contacted with the sample to be measured, and simultaneously plays a role in protecting the glucose concentration response module and the glucose viscosity response module and isolating the interference of external conditions.

In another preferred example, the shutter is connected to a timer, and the timer controls the opening and closing of the shutter by generating a periodic signal, so that the light beam formed by the optical lens can periodically vertically irradiate the sensitive film element on the surface of the first electrode.

In another preferred example, in the glucose viscosity response module, the first electrode and the second electrode are located on the surface of the substrate, and the sensitive membrane element is located on the first electrode.

In another preferred example, the first electrode and the second electrode are fixed on the surface of the substrate by metal evaporation, chemical modification and the like or by inert adhesives which do not affect the overall response.

In another preferred example, the sensitive membrane element is fixed on the first electrode by means of solution drop coating, physical adsorption, chemical modification and the like.

In another preferred example, the glucose viscosity response module is used for measuring the viscosity of the glucose solution to be measured in the reaction module.

In another preferred embodiment, the bottomless container is fixed on the substrate by an adhesive, so that the glucose viscosity response module and the glucose concentration response module are partially located in the bottomless container.

In another preferred embodiment, the adhesive for fixing the bottomless container comprises EVA or other thermosetting or thermoplastic resin with high strength, good insulation and no influence on response.

In another preferred example, in the glucose concentration response module, the third electrode (such as platinum-glucose oxidase-gold film working electrode), the fourth electrode (such as platinum electrode) and the reference electrode (such as silver-plated/silver chloride reference electrode) are positioned on the surface of the substrate, the metal (such as gold) on the third electrode is coated on the glucose oxidase layer in the form of a film, the working electrode of a platinum-glucose oxidase-gold film sandwich structure is formed, and the glucose concentration response module is partially positioned in the bottomless container.

In another preferred embodiment, the third electrode, the fourth electrode and the reference electrode are fixed on the surface of the substrate by metal evaporation, chemical modification and the like, or by inert adhesives which do not affect the overall response.

In another preferred example, the glucose concentration response module is used for measuring the concentration of the glucose solution to be measured in the reaction module.

In another preferred example, in the response measurement and analysis module, the measurement interface is connected with a glucose viscosity response module part, a glucose concentration response module part and the current meter which are positioned outside the bottomless container, and the current meter is connected with the data recording and analysis device through a data output port.

In another preferred embodiment, the response measuring and analyzing module is used for subsequent data processing and analysis.

In another preferred embodiment, during viscosity measurement, an excitation light source in a light source module emits periodic light to penetrate through a glucose solution, and then irradiates a sensitive membrane element on a first electrode, a periodic photocurrent is generated between the first electrode and a second electrode, the measured current response is recorded and drawn into a time-current period response curve, the obtained current responses of a plurality of periods are integrated with time, then an average value is obtained, an electric quantity value passing through the solution can be obtained, a standard curve of the relation between the electric quantity value and the viscosity of each solution is obtained by measuring a plurality of times on a plurality of liquids with different viscosities, and therefore the viscosity value of the solution is measured according to the electric quantity value and the standard curve of the solution to be measured.

In another preferred embodiment, when measuring the concentration, firstly, current is applied to the gold film on the platinum-glucose oxidase-gold film working electrode to dissolve the gold film, the glucose oxidase is exposed, the enzyme catalyzes the glucose redox reaction in the solution to generate current, and then the measured current response is recorded, so that the concentration value of the glucose in the solution to be measured is measured according to the standard curve of the current-concentration relation of the glucose.

In another preferred embodiment, the sensor first performs the glucose viscosity measurement and then the glucose concentration measurement. During viscosity measurement, the glucose concentration response module does not work; the glucose viscosity response module does not operate while the concentration measurement is being made.

It is to be understood that within the scope of the present invention, the above-described features of the present invention and those specifically described below (e.g., in the examples) may be combined with each other to form new or preferred embodiments. Not to be repeated herein, depending on the space.

Drawings

FIG. 1 is a system schematic of a sensor of the present invention.

Fig. 2 is a schematic diagram of a light source module according to an embodiment of the invention.

FIG. 3 is a schematic diagram of a glucose viscosity response module in an embodiment of the invention.

FIG. 4 is a schematic diagram of a glucose concentration response module in an embodiment of the invention.

FIG. 5 is a diagram of a response measurement and analysis module in an embodiment of the invention.

Fig. 6 is a schematic structural diagram of an embodiment of the present invention.

FIG. 7 is a graphical representation of data for an example viscosity measurement in an embodiment of the present invention.

FIG. 8 is a data diagram of an example concentration measurement in an embodiment of the invention.

Description of reference numerals:

10: a light source module;

20: a reaction module;

30: a glucose viscosity response module;

40: a glucose concentration response module;

50: a response measurement and analysis module;

11: a laser source;

12: an optical fiber;

13: an optical lens;

14: a shutter;

15: a timer;

21: a silicon dioxide substrate;

22: a bottomless container;

31: a gold electrode;

32: an aluminum electrode;

33: p3HT is PCBM blending membrane;

41: a platinum-glucose oxidase-gold thin film working electrode;

42: a platinum counter electrode;

43: silver plating/silver chloride reference electrode;

51: a measurement interface;

52: an ammeter;

53: data recording and analysis equipment.

Detailed Description

The inventor of the invention develops a sensor for measuring the viscosity and the concentration of a glucose solution for the first time through extensive and intensive research, wherein the viscosity measurement is based on the photoelectric effect of an organic semiconductor material, and the concentration measurement is based on a biosensing detection mechanism of enzyme specificity identification. The sensor can measure the viscosity and concentration of the glucose solution in the same system, firstly measures the viscosity of the glucose and then measures the concentration of the glucose, avoids the complicated steps caused by the independent measurement of the traditional glucose concentration and viscosity measurement in respective systems, and can reduce the using amount of required samples (the using amount can be reduced to 10-100 microliter orders of magnitude) and achieve high response speed, stable, reliable and high efficiency of detection results. The present invention has been completed on the basis of this finding.

Sensor for measuring viscosity and concentration of glucose solution

One aspect of the present invention is directed to a sensor for measuring the viscosity of a glucose solution and its concentration.

As shown in fig. 1, the sensor of the present invention includes a light source module 10, a reaction module 20, a glucose viscosity response module 30, a glucose concentration response module 40, and a response measurement and analysis module 50. Further, as shown in fig. 2, wherein the light source module 10 includes a laser source 11, an optical fiber 12, an optical lens 13, a shutter 14, and a timer 15; further wherein the reaction module 20 comprises a silicon dioxide substrate 21 and a bottomless container 22; further, as shown in fig. 3, wherein the glucose viscosity response module 30 comprises a gold electrode 31, an aluminum electrode 32, and a P3HT: PCBM blend membrane 33; further, as shown in fig. 4, the glucose concentration response module 40 includes a platinum-glucose oxidase-gold thin film working electrode 41, a platinum counter electrode 42, a silver-plated/silver chloride reference electrode 43; further, as shown in fig. 5, the response measurement and analysis module 50 includes a measurement interface 51, a current meter 52 and a data recording and analysis device 53 (computer, mobile phone, etc.).

Further, as shown in fig. 6, it is an overall design of the sensor for measuring the viscosity and concentration of glucose solution according to the present invention. Wherein, the excitation light emitted by the laser source 11 is coupled to an optical lens 13 through an optical fiber 12, and the optical lens 13 has a focusing function, and can focus the excitation light emitted by the laser source 11 into an excitation light beam; further, the shutter 14 is connected to a timer 15, and the timer 15 controls the opening and closing of the shutter 14 by generating a periodic signal, so that the excitation beam formed by the optical lens 13 can periodically and perpendicularly irradiate the P3HT: PCBM blend film 33. Further, the gold electrode 31 and the aluminum electrode 32 are grown on the surface of the silicon dioxide substrate 21, and the P3HT: PCBM blend film 33 only covers the gold electrode 31; the bottomless container 22 is fixed on the silicon dioxide substrate 21 through an adhesive such as EVA (ethylene-vinyl acetate copolymer) so that the glucose viscosity response module 30 is partially positioned in the bottomless container 22; further, the platinum-glucose oxidase-gold film working electrode 41, the platinum counter electrode 42 and the silver/silver chloride plated reference electrode 43 are positioned on the surface of the silicon dioxide substrate 21, gold is covered on a glucose oxidase layer in a film form, the glucose oxidase is fixed on the platinum electrode to form a working electrode of a platinum-glucose oxidase-gold film sandwich structure, and the glucose concentration response module 40 is partially positioned in the bottomless container 22; further, the solution to be measured is added into the bottomless circular container 22, so that the gold electrode 31, the aluminum electrode 32, the P3HT, the PCBM blended membrane 33, the platinum-glucose oxidase-gold thin film working electrode 41, the platinum counter electrode 42 and the silver-plated/silver chloride reference electrode 43 are covered and communicated with the solution to be measured; further, the glucose viscosity response module 30, the glucose concentration response module 40 and the current meter 52 which are located outside the bottomless container 22 are connected through the measurement interface 51, and the current meter 52 is connected to the data recording and analyzing device 53 through a data output port.

Further, subsequent data processing and analysis is performed by the response measurement and analysis module 50. When the viscosity is measured, the laser source is turned on, and the P3HT: PCBM blend membrane is excited by the excitation light to generate electrons, thereby generating photocurrent, and the shutter switch is controlled by the timer (or manually), so that a periodic current change is generated on the electrodes, and the periodic change is collected and displayed on the device by the response measurement and analysis module 50. The measured current response is recorded and drawn into a time-current cycle response curve, the obtained current responses in a plurality of cycles are integrated, then the average value is obtained, the solution electric quantity value can be obtained, and a standard curve of the electric quantity-viscosity relation of each solution is obtained by measuring a plurality of times of liquids with different viscosities, so that the viscosity value of the solution is measured according to the electric quantity value of the sample to be measured and the standard curve, and at the moment, because the gold film on the platinum-glucose oxidase-gold film working electrode 41 is not electrified and dissolved, only the viscosity response exists at the moment, so that the viscosity of the solution is measured firstly; when the concentration is measured after the viscosity measurement is finished, the platinum-glucose oxidase-gold film working electrode 41 is reversely electrified through the response measurement and analysis module 50, so that the gold film is dissolved, the glucose oxidase on the electrode is exposed at the moment, the glucose in the solution to be measured is catalyzed to be oxidized and decomposed to generate electrons, and then current is generated, and the current response is collected and displayed on the equipment through the response measurement and analysis module 50. The method comprises the steps of measuring glucose solutions with different concentrations for multiple times to obtain a standard curve of a current-concentration relation of glucose, recording current response of a solution to be measured, and drawing the current response into a time-current response curve, thereby realizing measurement of the concentration value of the glucose in the solution to be measured

The main advantages of the invention include:

(1) The sensor of the invention measures the glucose concentration and the viscosity in sequence in the same system. The viscosity measurement is different current responses generated by the photoelectric sensitive device under different solution conditions, so that the viscosity of the solution to be measured can be accurately and indirectly measured, and the reliability of the viscosity measurement is ensured; the concentration measurement is the specific identification of glucose by glucose oxidase. Traditional concentration and viscosity measurements require separate measurements in both systems.

(2) According to the sensor, the viscosity is not required to be measured through a relatively accurate moving part, so that the interference of external movement factors is eliminated; meanwhile, the measurement can be carried out only by covering the electrode with the liquid to be measured to form a loop, so that the volume of the liquid to be measured is greatly reduced, the cost is reduced, and compared with the common viscosity measurement, the harsh measurement condition is simplified.

(3) The sensor greatly reduces the volume of a measuring system, improves the portability of the system, can realize portable measurement by matching with mobile equipment, and has more efficient measuring process.

(4) The sensor of the invention further measures the concentration of the glucose contained in the glucose solution after measuring the viscosity of the glucose solution, avoids the interference of a new substance generated by the oxidation reaction of the glucose when the concentration is measured firstly on the subsequent viscosity of the glucose solution, and can be matched with mobile equipment to realize the portable measurement of the concentration and the viscosity of the glucose.

(5) After the response measurement and analysis module 50 is adjusted and debugged for the first time, the subsequent measurement only needs manual operation of the laser source and reverse power-on control of the gold film, so that the sensor is easy to operate and simple and convenient to operate.

The invention is further illustrated with reference to specific embodiments. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Experimental procedures in the following examples, where specific conditions are not indicated, are generally carried out according to conventional conditions, for example as recommended by the manufacturer. Unless otherwise indicated, percentages and parts are by weight.

The materials and reagents used in the examples of the present invention are commercially available products unless otherwise specified.

Examples

As shown in fig. 1, the sensor of the present invention includes a light source module 10, a reaction module 20, a glucose viscosity response module 30, a glucose concentration response module 40, and a response measurement and analysis module 50. Further, as shown in fig. 2, wherein the light source module 10 includes a laser source 11, an optical fiber 12, an optical lens 13, a shutter 14, and a timer 15; further wherein the reaction module 20 comprises a silicon dioxide substrate 21 and a bottomless container 22; further, as shown in FIG. 3, wherein the glucose viscosity response module 30 comprises a first electrode (gold electrode 31), a second electrode (aluminum electrode 32), and a P3HT: PCBM blend membrane 33; further, as shown in fig. 4, the glucose concentration response module 40 includes a third electrode (platinum-glucose oxidase-gold thin film working electrode 41), a fourth electrode (platinum counter electrode 42), a reference electrode 43 (silver/silver chloride plated electrode); further, as shown in fig. 5, the response measurement and analysis module 50 includes a measurement interface 51, a current meter 52, and a data recording and analysis device 53 (such as a computer, a mobile phone, etc.).

And the gold electrode 31, the aluminum electrode 32, the platinum-glucose oxidase-gold thin film working electrode 41, the platinum counter electrode 42 and the silver/silver chloride plated reference electrode 43 are arranged in parallel in the reaction module (P3 HT: PCBM blend film 33 is positioned on the gold electrode 31, the light source module is positioned right above the gold electrode 31, the response measurement and analysis module is respectively connected with the glucose viscosity response module and the glucose concentration response module through measurement interfaces, and the glucose viscosity response module and the glucose concentration response module are not connected with each other.

Further, as shown in fig. 6, is an overall design of the sensor for measuring the viscosity of the glucose solution and the concentration thereof based on the photoelectric response of the present invention. Wherein, the excitation light emitted by the laser source 11 is coupled to an optical lens 13 through an optical fiber 12, and the optical lens 13 has a focusing function, and can focus the excitation light emitted by the laser source 11 into an excitation light beam; in a preferred embodiment, the laser source in the light source module can emit light with a specific wavelength, which belongs to the absorption wavelength of the P3HT: PCBM blend film 33. Further, the shutter 14 is connected to a timer 15, and the timer 15 controls the opening and closing of the shutter 14 by generating a periodic signal, so that the excitation beam formed by the optical lens 13 can periodically and perpendicularly irradiate the P3HT: PCBM blend film 33. Further, the gold electrode 31 and the aluminum electrode 32 are fixed on the surface of the silicon dioxide substrate 21 by a metal evaporation method (metal evaporation is a specific method, and can be directly put into a machine for metal evaporation), and the P3HT: PCBM blend film 33 is only coated on the gold electrode 31; the bottomless container 22 is fixed on the silicon dioxide substrate 21 through an adhesive such as EVA (ethylene vinyl acetate), so that the glucose viscosity response module 30 is partially positioned in the bottomless container 22; further, the platinum-glucose oxidase-gold thin film working electrode 41 is respectively fixed on the surface of the silicon dioxide substrate 21 by three methods of metal evaporation, physical adsorption and chemical modification, the platinum counter electrode 42 is fixed on the surface of the silicon dioxide substrate 21 by a metal evaporation method, and the silver/silver chloride reference electrode 43 is firstly subjected to metal evaporation and then chemical modification, for the platinum-glucose oxidase-gold thin film working electrode 41, firstly metal platinum is evaporated, then a glucose oxidase layer is adsorbed on the platinum layer by physical adsorption (realized by adding adsorbing materials such as chitosan, carbon nano tubes and the like), and finally gold is covered on the glucose oxidase layer in a thin film form by chemical modification to form a working electrode with a platinum-glucose oxidase-gold thin film sandwich structure, and the glucose concentration response module 40 is partially positioned in the bottomless container 22; further, the solution to be measured is added into the bottomless circular container 22, so that the gold electrode 31, the aluminum electrode 32, the P3HT, the PCBM blended membrane 33, the platinum-glucose oxidase-gold thin film working electrode 41, the platinum counter electrode 42 and the reference electrode 43 (silver-plated/silver chloride electrode) are covered and communicated with the solution to be measured; further, the glucose viscosity response module 30, the glucose concentration response module 40 and the current meter 52 which are located outside the bottomless container 22 are connected through the measurement interface 51, and the current meter 52 is connected to the data recording and analyzing device 53 through a data output port.

Further, subsequent data processing and analysis is performed by the response measurement and analysis module 50. When the viscosity is measured, the laser source is turned on, and at the time, the P3HT: PCBM blend film is excited by the excitation light to generate electrons, so as to generate a photocurrent, and a timer (or manually) controls the shutter switch, so that a periodic current change is generated on the electrode, and the periodic current change is collected and displayed on a device (such as a mobile phone, a computer, and the like) through the response measurement and analysis module 50. Recording the measured current response, drawing the current response into a time-current period response curve, integrating the obtained current responses in a plurality of periods, then averaging to obtain a solution electric quantity value, and obtaining a standard curve of the electric quantity-viscosity relation of each solution by measuring a plurality of times on a plurality of liquids with different viscosities, so that the viscosity value of the solution is measured according to the electric quantity value of the sample to be measured and the standard curve, wherein at the moment, only the viscosity response exists because the gold film on the platinum-glucose oxidase-gold film working electrode 41 is not electrified and dissolved, and the viscosity of the solution is measured firstly; when the concentration is measured after the viscosity measurement is finished, the platinum-glucose oxidase-gold film working electrode 41 is reversely electrified through the response measurement and analysis module 50, so that the gold film is dissolved, the glucose oxidase on the electrode is exposed at the moment, the glucose in the solution to be measured is catalyzed to be oxidized and decomposed to generate electrons, and then current is generated, and the current response is collected and displayed on the equipment through the response measurement and analysis module 50. The method comprises the steps of measuring glucose solutions with different concentrations for multiple times to obtain a standard curve of a current-concentration relation of glucose, recording current response of a solution to be measured, and drawing the current response into a time-current response curve, thereby realizing measurement of the concentration value of the glucose in the solution to be measured

In the present invention, a specific measurement example procedure is as follows:

a certain volume (e.g. 1 mL) of solution (containing glucose) with different viscosities is added into bottomless container 22, light source module 10 is started to generate an excitation beam with a period of 8s (5 s on, 3s off) to irradiate glucose viscosity response module 30, and at this time, P3HT: the PCBM blend film is excited by the excitation light to generate photoelectrons and generate photocurrent, which causes a periodic current variation on the electrodes, and the periodic current variation is collected and displayed on a device (e.g., a mobile phone or a computer) through the response measurement and analysis module 50. The time-current cycle response curve (current data and time are plotted as images in a conventional manner) shown in fig. 7 is obtained by a data recording and analyzing device 53 (e.g., a mobile phone, a computer, etc.), and the electric quantity value calculated by integrating the response curve is compared with the electric quantity-viscosity relation curve of the solution, so as to determine the viscosity of the solution. After the viscosity measurement is finished, current is applied to the gold film on the platinum-glucose oxidase-gold film working electrode 41 to dissolve the gold film, the glucose oxidase on the platinum electrode is exposed, glucose solution with a certain concentration (such as 0.1 mol/L) is sequentially dripped into the solution, the glucose oxidase catalyzes the glucose in the solution to be measured to be oxidized and decomposed to generate electrons so as to generate current, and the current response is collected and displayed on equipment (such as mobile phone or computer equipment) through the response measurement and analysis module 50. The time-current response curve (the current data and time are plotted as an image in a conventional manner) shown in fig. 8 is obtained by the data recording and analyzing device 53 (e.g., a mobile phone, a computer, or the like), and the glucose concentration is determined by comparing the magnitude of the response value with the current-concentration relationship curve of glucose.

The response is obvious, the relation between the electric quantity and the viscosity, the current and the concentration of the glucose solution can be accurately reflected, the glucose concentration contained in the glucose solution is further measured after the viscosity of the glucose solution is measured, and the interference of the glucose on the viscosity of the glucose solution caused by oxidation reaction when the concentration is measured in advance is avoided.

All documents referred to herein are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention can be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the present invention as defined by the appended claims.

Claims (12)

1. A sensor for measuring the viscosity and concentration of a glucose solution, comprising: a light source module, a reaction module, a glucose viscosity response module, a glucose concentration response module and a response measurement and analysis module, wherein,

the light source module comprises an excitation light source, an optical fiber, an optical lens, a shutter and a timer; the reaction module comprises a substrate and a bottomless container;

the glucose viscosity response module comprises a first electrode, a second electrode and a sensitive membrane element;

the glucose concentration response module comprises a third electrode, a fourth electrode and a reference electrode;

the response measuring and analyzing module comprises a measuring interface, an ammeter and a device for data recording and analyzing;

the first electrode, the second electrode, the third electrode, the fourth electrode and the reference electrode are arranged in the reaction module in parallel, the sensitive membrane element is positioned on the first electrode, the light source module is positioned right above the first electrode, and the response measurement and analysis module is respectively connected with the glucose viscosity response module and the glucose concentration response module.

2. The sensor of claim 1, wherein the glucose viscosity response module and the glucose concentration response module are not connected to each other.

3. The sensor of claim 1, wherein the sensitive membrane element is an organic semiconductor material, affixed to the first electrode by one or more methods selected from the group consisting of: solution drop coating, physical adsorption, chemical modification and the like.

4. The sensor of claim 1, wherein the excitation light source in the light source module emits light of a specific wavelength that is within an absorption wavelength of the sensitive film element.

5. The sensor of claim 1, wherein the first electrode comprises an electrode of one or more materials selected from the group consisting of: gold or other materials which do not influence photoelectric response, have stable chemical properties and good conductive function.

6. The sensor of claim 1, wherein the second electrode comprises an electrode of one or more materials selected from the group consisting of: aluminum, platinum, or other materials that are chemically inert, electrically conductive, and stable, and do not interfere with the reactions at the other electrodes.

7. The sensor of claim 1, wherein the third electrode comprises a platinum-glucose oxidase-gold thin film working electrode.

8. The sensor of claim 1, wherein the fourth electrode comprises platinum or another electrode made of a material that is chemically inert, electrically conductive, and stable, and does not affect the reaction at the other electrode, as a counter electrode to the third electrode.

9. The sensor of claim 1, wherein the reference electrode comprises a silver/silver chloride electrode or a mercury/mercury chloride electrode made of a material having a stable electrode potential, good reproducibility and only a single reversible reaction.

10. The sensor as claimed in claim 1, wherein in measuring viscosity, the excitation light source module emits periodic light to the sensitive membrane element on the first electrode, periodic photocurrent is generated between the first electrode and the second electrode, a time-current period response curve is drawn by recording the measured current response, the obtained current responses of a plurality of periods are integrated for time, then an average value is taken to obtain an electric quantity value of a passing solution, and a standard curve of the electric quantity value-viscosity relationship of each solution is obtained by measuring a plurality of times of liquids with different viscosities, so that the viscosity value of the solution to be measured is measured according to the electric quantity value of the solution to be measured and the standard curve.

11. The sensor of claim 1, wherein when measuring the concentration, a current is applied to the gold film on the platinum-glucose oxidase-gold film working electrode to dissolve the gold film, the glucose oxidase is exposed, the enzyme catalyzes the glucose oxidation reaction in the solution to generate a current, and the measured current response is recorded, so that the concentration value of the glucose in the solution to be measured is measured according to a standard curve of the current-concentration relationship of the glucose.

12. The sensor of claim 1, wherein the sensor performs the glucose viscosity measurement prior to the glucose concentration measurement; during viscosity measurement, the glucose concentration response module does not work; the glucose viscosity response module does not operate while the concentration measurement is being made.

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