CN115702949A

CN115702949A - Wound aseptic liquid dressing for blocking and inactivating SARS-COV-2 virus

Info

Publication number: CN115702949A
Application number: CN202110905523.0A
Authority: CN
Inventors: 请求不公布姓名
Original assignee: Weidun Biotechnology Guangzhou Co ltd
Current assignee: Weidun Biotechnology Guangzhou Co ltd
Priority date: 2021-08-05
Filing date: 2021-08-05
Publication date: 2023-02-17

Abstract

The invention discloses a wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus, which is characterized by comprising the following raw materials: 97.3 to 98.84 weight percent of hypochlorous acid water, 0.86 to 0.90 weight percent of sodium chloride, 0.1 to 1.0 weight percent of stabilizer and 0.2 to 0.8 weight percent of chelating agent. The hypochlorous acid water is non-electrolytic weakly acidic hypochlorous acid water, the concentration is 100 mg/L-200 mg/L, and the PH is 4.5-6.5; the sodium chloride is food grade; the stabilizer is one or a mixture of two of sodium dihydrogen phosphate and sodium hexametaphosphate, and is food grade; the chelating agent is one or a mixture of ethylene diamine tetraacetic acid and disodium ethylene diamine tetraacetic acid, and is food grade or pharmaceutical grade. The wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus is provided aseptically, and plays a role of physical barrier by forming a protective layer on the surface of a wound surface. Used for nursing chronic wound and peripheral skin; is used for nursing non-chronic wounds such as small wounds, bruises, cut wounds and the like and peripheral skin. When wound care is carried out, pathogenic microorganisms are blocked and killed, and particularly SARS-COV-2 virus is blocked and inactivated.

Description

Wound aseptic liquid dressing for blocking and inactivating SARS-COV-2 virus

Technical Field

The invention belongs to the field of wound dressings in 14-infusion, nursing and protection instruments in medical instruments, and particularly relates to a wound sterile liquid dressing for blocking and inactivating SARS-COV-2 virus.

The wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus is provided aseptically, and plays a role of physical barrier by forming a protective layer on the surface of a wound surface. Used for nursing chronic wound and peripheral skin; is used for nursing non-chronic wounds such as small wounds, bruises, cut wounds and the like and peripheral skin. When wound care is carried out, pathogenic microorganisms are blocked and killed, and particularly SARS-COV-2 virus is blocked and inactivated.

Background

The wound refers to the injury of the body caused by skin laceration or mucosa breakage, the function of the whole skin and mucosa is to prevent the invasion of bacteria on the surface of the skin and the periphery thereof, and if the barrier is lost, a warm, moist and nutrient-rich living environment is provided for microorganisms, and an opportunity for colonization, growth and propagation is provided.

Wounds in the broad sense are divided into acute wounds and chronic wounds.

a) Acute wounds refer to intact skin injuries including surgical wounds, bites, burns, abrasions, wounds, and the like that generally heal faster;

b) Chronic wounds are caused by endogenous mechanisms, and generally comprise venous ulcers (venous leg ulcers, rotten legs), arterial ulcers (arterial leg ulcers), diabetic ulcers (diabetic feet), traumatic ulcers (oral mucosa ulcers), pressure ulcers (I-IV pressure sores), cervical ulcers (cervical erosion), tinea pedis ulcers and the like, and are slow to heal.

Almost all wounds can be contaminated with bacteria in air or bacteria from tissues or organs near the wounds to different degrees by the colonization of pathogenic bacteria, particularly, the oral cavity, the intestinal tract and the genitourinary tract are rich and diversified in bacteria, and the types of bacteria infected by the wounds are closely related to the flora around the damaged parts.

Generally, colonizing wounds heal successfully, but some do not. Whether a chronic wound with a certain bioburden can heal depends on the number of bacteria, their toxicity and whether they proliferate (Swanson 2015).

As bacteria multiply within the wound, the normal inflammatory phase is prolonged by the release of harmful enzymes, oxygen radicals and inflammatory cells; these deleterious factors ultimately lead to tissue destruction and further deterioration of the wound (Edwards Jones 2013). This stage has been previously referred to as severe colonization (World Union of round health Societies 2008).

In the past, severe colonisation wounds were defined as containing < 10^5 Colony Forming Units (CFU)/g tissue, this definition being criticized as too simple to take into account the type and toxicity of microorganisms in the wound (Swanson 2015).

The wound infection, as long as bacteria are planted on the wound, is possible to be potential pathogenic bacteria, and is closely related to various factors such as infection risk, occurrence of infection phenomena, wound conditions, types and quantity of pathogenic bacteria, wound treatment process, host immunity, postoperative antibiotic use and the like.

a) Surgical wound infection: surgical wound infection accounts for about 25% of hospital infection, wherein 60-80% of surgical wound infection is hand incision infection, and main pathogenic bacteria for infection are staphylococcus aureus, coagulase negative staphylococcus, escherichia coli, enterococcus, enterobacter, pseudomonas aeruginosa and the like;

b) Acute soft tissue infection: acute soft tissue infections include cutaneous abscesses, wounds, and necrotizing tissue infections, 25-30% of which are reported by brook to be the result of a single staphylococcus aureus infection, and studies have shown that 30-50% of cutaneous abscesses, 50% of wounds, and 47% of necrotizing tissue infections are infections by a variety of bacteria (aerobic and anaerobic), including streptococcus digestions, bacteroides fragilis, streptococcus pyogenes, clostridium perfringens, escherichia coli, proteus, and the like;

c) Wound infection of animals: it is reported that human bites have an infection rate of 10 to 50%, dog bite infection rate of 20%, and cat bite infection rate of 30 to 50% depending on the bite site and severity, and these bacteria include clostridium tetani, clostridium carbonarium, staphylococcus aureus, streptococcus digestans, bacteroides, porphyromonas, prevotella, rabies virus, etc.;

d) Infection of burn and scald: infection is the main complication of burn, and 75% of death cases caused by burn complications are related to infection, and the main bacteria are staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, enterococcus, candida, streptococcus digestans, bacteroides, klebsiella, propionibacterium acnes and the like;

e) Diabetic ulcer (diabetic foot) infection: foot ulcers associated with diabetic feet have a high incidence of infection, and proper debridement and pressure relief of necrotic tissue have important significance in reducing the infection rate, and in diabetic foot ulcer infection, staphylococcus aureus is the main pathogenic bacterium, and staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, enterococcus, streptococcus, bacteroides, prevotella and the like are the secondary causes;

f) Venous ulcer (venous leg ulcer of lower limb) infection: staphylococcus aureus is a common pathogenic bacterium, others are digestive streptococci, pigmented or nonpigmented gram-negative bacilli;

g) Pressure sore (decubitus) ulcer infection: the development of pressure sores and bedsores can cause skin erosion, ischemia and necrosis of local tissues, about 25 percent of the pressure sores and bedsores can develop osteomyelitis and bacteremia, and staphylococcus aureus, streptococcus digesta, bacteroides and pseudomonas aeruginosa are main pathogenic bacteria.

Biofilms are a dynamic community of microorganisms, such as bacteria and fungi, that live in protective matrices composed of self-secreted polysaccharides and proteins (Wolcott 2008). Until recently, there was much debate about the presence of biofilm in wounds (Bourdillon 2017) that was not visible to the naked eye in wounds (Philips 2010).

The authors believe that biofilm is present in up to 60% of chronic wounds and is one of the factors responsible for delayed healing (James 2008). However, it is also believed that wounds containing biofilm heal normally and no infection occurs (Percival 2015). Further studies to confirm wound biofilms need to be conducted.

In recent years, many studies at home and abroad show that most infected wounds can form bacterial biofilms in the infected wounds, the bacterial biofilms are in very important direct connection with the drug resistance of bacteria and the healing difficulty of the wounds, and the biofilms have very strong drug resistance and can survive for a long time, thereby bringing obvious difficulty to treatment. Biofilm bacteria are significantly different from free or planktonic bacteria in morphological and physiological properties. The bacteria in the membrane, also known as "indigenous bacteria", are more than 1000 times more resistant to the disinfectant than the free existing individual bacteria due to the encapsulation of the membrane, and are difficult to remove by conventional disinfection methods. Therefore, the traditional Chinese medicine composition can inhibit the formation of bacterial biofilms and effectively remove the formed biofilms, and becomes an effective measure for clinically treating wounds and wound infection.

The bacterial load of the local infected wound is reduced, and a wound bed of the local infected wound is easy to generate a large amount of carrion, so that the bacterial quantity is increased. The carrion reappears soon after debridement, and granulation tissues show abnormal dark red. The susceptibility of wound tissue to bleeding, odor and stasis of healing also indicates the presence of a high bacterial load (hewinh 2014).

Topical antimicrobial products should be used as a first line of treatment if the number of bacteria in a topically infected wound is suspected to be increasing or if a biofilm is present. This will prevent delayed healing and/or worsening of infection. If biofilm is suspected, systemic antibiotics are not recommended because biofilms may develop resistance; and systemic antibiotic treatment is not necessarily effective because it is difficult to penetrate and disrupt biofilms (WUWHS 2008). Treatment should focus on disrupting biofilms, primarily through continuous and regular debridement and use of topical antimicrobial dressings (Wounds UK 2013).

The wound debridement and antibiosis are mainly carried out by using soap water (added with hydrogen peroxide) to repeatedly wash a wound surface and a wound, and then using normal saline to wash the wound surface and the wound, thereby removing obvious foreign matters, blood clots and fallen necrotic tissues. After the above treatment, the wound is finally disinfected by a disinfectant. The wound debridement method is too cumbersome and time consuming, and the traditional disinfectants cannot effectively remove the biofilm formed on the surface of the wound.

The last century has seen fewer antimicrobial products available for the treatment of wound infections, one of which is iodine, which was introduced to the market in the 1980 s and contains 10% povidone-iodine, equivalent to 1% available iodine (sibbalad 2017).

The effectiveness of iodine in wound treatment is controversial because of concerns about its toxicity and retardation of granulation tissue growth. However, some studies suggest that iodine-containing products are effective antimicrobial agents with no associated adverse effects, comparable to any other antimicrobial wound product (sibbard 2017.

Human pathogens are becoming resistant to antibiotics that have evolved over the past century, and the common symptoms of infection that once could be treated by antibiotics are now fatal, even with best practice therapy. One approach to addressing this risk is to disinfect the intact skin or open wound to effectively prevent the proliferation of the pathogen before it develops into a life-threatening infection. However, the current disinfection methods are relatively easy to generate drug resistance, toxic to human tissues and can damage the environment after use.

Hypochlorous acid (HCLO) is a small molecular chemical substance, is weakly acidic, has strong oxidizing property and strong penetrability, generally exists in an aqueous solution to form hypochlorous acid water, and cannot exist independently in nature.

Hypochlorous acid has a virus and bacteria killing mechanism, and has strong oxidizability and strong penetrability.

a) Extremely strong penetrability of hypochlorous acid: can directly penetrate the peripheral structure cell wall and cell membrane of microbial cell, enter the inner core of the microbe and destroy the inner core DNA or RNA, thereby killing various viruses and bacteria with the killing rate of 99.999 percent. After the action, it is reduced to water and traces of chloride.

Human and animal cells belong to giant cells, and hypochlorous acid molecules cannot penetrate through the giant cells.

b) Hypochlorous acid is very oxidizing: can oxidize and decompose amino acids in enzyme protein on the cell membrane surface of microorganism, so as to deactivate the enzyme protein, and cause metabolic dysfunction and death. Viruses and germs (bacteria, fungi and molds) are most prokaryotic cell organisms, and enzyme systems of the viruses and the germs are mostly distributed on the surface of a cell membrane and are easily attacked by hypochlorous acid oxidation to lose activity. Hypochlorous acid breaks down bacteria and other microorganisms in the sterilization process through peroxidation, and the products are water, chloride, organic sugar and trace carbon dioxide nontoxic substances.

Human and animal are eukaryotic cell organisms, most of the enzyme systems are hidden in organelles, and hypochlorous acid cannot directly contact the organelles.

The hypochlorous acid water has strong killing power to viruses and germs and can kill various microorganisms, including prion, bacterial spores and bacterial spores; mycobacteria, hydrophilic viruses, fungi; gram negative bacteria, large non-cytoviruses; gram-positive bacteria, and lipophilic viruses.

Disclosure of Invention

The invention aims to provide a wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus.

The purpose of the invention is realized by the following technical scheme:

the invention provides a wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus, which is characterized in that:

a) A wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus is characterized in that the raw materials comprise: 97.3 to 98.84 weight percent of hypochlorous acid water, 0.86 to 0.90 weight percent of sodium chloride, 0.1 to 1.0 weight percent of stabilizer and 0.2 to 0.8 weight percent of chelating agent.

The hypochlorous acid water is non-electrolytic weakly acidic hypochlorous acid water, the concentration is 100 mg/L-200 mg/L, and the PH is 4.5-6.5; the sodium chloride is food grade; the stabilizer is one or a mixture of two of sodium dihydrogen phosphate and sodium hexametaphosphate, and is food grade; the chelating agent is one or a mixture of ethylene diamine tetraacetic acid and disodium ethylene diamine tetraacetic acid, and is food grade or pharmaceutical grade.

b) The preparation method of the wound sterile liquid dressing for obstructing and inactivating the SARS-COV-2 virus is characterized by comprising the following steps:

step 1: preparing purified water, and conveying the purified water to a hypochlorous water generator;

step 2: preparing a hydrochloric acid solution, namely preparing hydrochloric acid with the concentration of 7.5-8.5%;

and step 3: preparing a sodium hypochlorite solution; preparing sodium hypochlorite into 8-9% sodium hypochlorite;

and 4, step 4: preparing hypochlorous acid water, namely adding the hydrochloric acid solution in the step 2 and the sodium hypochlorite solution in the step 3 into the hypochlorous acid water generator in the step 1 at the same time to prepare hypochlorous acid water;

and 5: after the hypochlorous acid water in the step (4) is inspected to be qualified, storing the hypochlorous acid water in a storage tank in a sealed mode, and storing the hypochlorous acid water in a shading mode;

and 6: adding a chelating agent into the hypochlorous acid water in the step 5, and stirring for 5-20 min;

and 7: adding a stabilizer into the hypochlorous acid water in the step 6, and stirring for 5-20 min;

and 8: adding sodium chloride into the hypochlorous acid water in the step 7, stirring for 5-20 min, and standing for 2-3h;

and step 9: and 8, detecting the wound sterile liquid dressing after standing in the step 8, filling after the detection is qualified, sealing the finished product, and storing in a dark place to prepare the wound sterile liquid dressing for blocking and inactivating the SARS-COV-2 virus.

The invention has the following beneficial effects:

the product has the advantages of sterility, no toxicity, no irritation, no sensitization, no mutagenicity, strong inhibition and killing power, high stability and weak acidity.

a) Sterile

According to the detection of the microbiological analysis detection center of Guangdong province and the microbiological research institute of Guangzhou city, the detection conclusion is as follows: the sterility test meets the requirements of 'sterility' in GB27951-2011 hygienic requirements for skin disinfectants.

b) Non-toxic grade

According to the detection of the microbiological analysis detection center of Guangdong province and the microbiological research institute of Guangzhou city, the detection conclusion is as follows: acute oral toxicity test is nontoxic grade, and acute inhalation toxicity test is actual nontoxic grade.

c) Has no irritation

According to the detection of the microbiological analysis detection center of Guangdong province and the microbiological research institute of Guangzhou city, the detection conclusion is as follows: the test of damaged skin has no irritation, the test of acute eye irritation has no irritation, the test of vaginal mucosa irritation has no irritation for many times, and the test of complete skin irritation has no irritation for many times.

d) No sensitization

According to the detection of the microbiological analysis detection center of Guangdong province and the microbiological research institute of Guangzhou city, the detection conclusion is as follows: the skin allergy test has no sensitization.

e) No mutagenicity

According to the detection of the microbiological analysis detection center of Guangdong province and the microbiological research institute of Guangzhou city, the detection conclusion is as follows: one mutagenic test was negative.

f) Strong resistance to killing

1) According to the detection of the microbiological analysis detection center of Guangdong province and the microbiological research institute of Guangzhou city, the detection conclusion is as follows: the killing logarithm of the staphylococcus aureus and the pseudomonas aeruginosa is more than or equal to 5, and the killing rate is more than or equal to 99.999 percent; the killing logarithm of the candida albicans is more than or equal to 4, and the killing rate is more than or equal to 99.99%.

2) According to the analysis and detection of Wuhan virus institute, the medicine is characterized by having the following characteristics that the detection conclusion of the medicine for the new crown pneumonia virus SARS-COV-2 with the concentration of 200mg/L is as follows:

TABLE Effect table against SARS-COV-2 of new coronary pneumonia virus at different time

g) High stability

According to the detection of the institute of microorganisms in Guangzhou city, the detection conclusion is as follows: the reduction rate of available chlorine in one year reaches 3.3 percent.

h) Weak acidity

According to the detection of the microbiological analysis detection center of Guangdong province and the microbiological research institute of Guangzhou city, the detection conclusion is as follows: the pH value is 4.5-6.5.

The invention relates to a wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus, which has the following application range and application method:

a) The application range is as follows: a wound sterile liquid dressing for blocking and inactivating SARS-COV-2 virus is suitable for the expected application of ' 08 liquid and paste dressing ' in ' 10 wound dressing ' in ' 14 infusion, nursing and protection apparatus ' in medical apparatus classification catalogue ' (2017), is provided aseptically, and plays a role of physical barrier by forming a protective layer on the surface of wound. Used for nursing chronic wound and peripheral skin; is used for nursing non-chronic wounds such as small wounds, bruises, cut wounds and the like and peripheral skin. When wound care is carried out, pathogenic microorganisms are blocked and killed, and particularly SARS-COV-2 virus is blocked and inactivated.

b) The using method comprises the following steps: the invention relates to a method for using a wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus, which comprises the following steps:

1) Wound opening and wound surface debridement: the stock solution of the product is directly used for debridement of wounds and peripheral tissues thereof, and no clear water is needed for washing after debridement.

2) Wet dressing of sore and wound surface: it is used according to the clinical operation standard according to the area of the wound and the wound opening.

-exposed sores and wounds: after debridement, sufficiently spraying the affected part and the adjacent peripheral area until the affected part is uniformly covered by the liquid dressing, and using the dressing according to the clinical operation specification for 2-3 times a day according to the area of the wound opening and the wound surface;

wound and sore dressing: the dressing is convenient to take down when the dressing is taken down and the dressing is soaked in the stock solution of the dressing and is adhered to the wound opening and the wound surface; then using the stock solution of the product to clean necrotic tissues and secretions by matching with a sterile cotton ball or medical gauze; when in dressing, one side of the dressing contacting with the wound opening and the wound surface is sprayed with moisture and is applied to the affected part; during the dressing change interval, the stock solution can be directly sprayed on the dressing according to the requirements of the moisture degree of the wound and the wound.

Detailed Description

The preferred embodiments of the present invention are described below, and it should be understood that the embodiments described herein are only for the purpose of illustrating and explaining the present invention and are not intended to limit the present invention.

The first embodiment is as follows:

a) A wound aseptic liquid dressing for obstructing and inactivating SARS-COV-2 virus is characterized by comprising the following raw materials: 97.99wt% of hypochlorous acid water, 0.86% of sodium chloride, 0.6% of stabilizing agent and 0.55% of chelating agent.

The hypochlorous acid water is non-electrolytic weakly acidic hypochlorous acid water, the concentration is 180 mg/L-200 mg/L, and the PH is 4.5-6.5; the sodium chloride is food grade; the stabilizer is a mixture of two of sodium dihydrogen phosphate and sodium hexametaphosphate (the proportion is 1: 2), and the two are both food grade; the chelating agent is disodium ethylene diamine tetraacetate and is food grade or pharmaceutical grade.

step 1: preparing purified water and conveying the purified water to a hypochlorous water generator;

and 2, step: preparing a hydrochloric acid solution, namely preparing hydrochloric acid with the concentration of 7.5-8.5%;

and step 3: preparing a sodium hypochlorite solution; preparing sodium hypochlorite into sodium hypochlorite with the concentration of 8-9%;

step 6: adding a chelating agent into the hypochlorous acid water obtained in the step 5, and stirring for 20min;

and 7: adding the stabilizer into the hypochlorous acid water in the step 6, and stirring for 15min;

and step 8: adding sodium chloride into the hypochlorous acid water in the step 7, stirring for 10min, and standing for 2h;

and step 9: and 8, detecting the wound sterile liquid dressing after standing in the step 8, filling after the detection is qualified, sealing the finished product, and storing in a dark place to obtain the wound sterile liquid dressing for blocking and inactivating the SARS-COV-2 virus.

c) The wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus is detected by microbiological research institute in Guangzhou city, and is characterized by comprising the following characteristics:

1) The pH (25 ℃) of this sample was 6.18;

2) The arsenic content of the sample is less than 0.01mg/kg, the mercury content is less than 0.0021mg/kg, and the lead content is less than 1.5mg/kg, so that the sample meets the requirements of technical safety standards of cosmetics (2015 edition);

3) The effective chlorine component of the sample is 198mg/L;

4) Placing the sample in a constant temperature box at 54 ℃ for 14 days (testing the validity period of one year), wherein the color has no obvious change, no sediment or suspended matters are generated, the content of effective components after placing is 186.5mg/L, the reduction rate of the effective components of the sample is 5.8 percent, the reduction rate of the effective components is less than or equal to 10 percent, and the validity period of storing the sample can be determined as one year;

5) The total bacterial colony number, the detection results of mould, saccharomycetes, pseudomonas aeruginosa, staphylococcus aureus and beta hemolytic streptococcus of the sample meet the requirements of GB27951-2011 'hygienic requirements for skin disinfectants';

6) The aseptic detection test result of the sample meets the requirements of GB27951-2011 sanitary requirements for skin disinfectants;

7) 1% lecithin, 3.0% Tween 80 and 1% sodium thiosulfate PBS solution are used as a neutralizer, the sample stock solution acts for 0.5min, the killing logarithm of staphylococcus aureus and pseudomonas aeruginosa in suspension is more than 5, the killing rate is more than or equal to 99.999%, and the requirements of the disinfection technical specification (2002 edition) are met;

8) 1% lecithin, 3.0% Tween 80 and 1% sodium thiosulfate in PBS solution are used as neutralizer, the sample stock solution acts for 0.5min, the killing logarithm of Candida albicans in suspension is more than 4, the killing rate is more than or equal to 99.99%, and the requirement of disinfection technical Specification (2002 edition) is met;

9) In the field test, the sample is sprayed on the skin for 1min, the average killing logarithm of the natural bacteria on the surface of the skin is more than 1, the positive control group has more bacteria growth, and the negative control group has aseptic growth, thereby meeting the requirements of the disinfection technical specification (2002 edition);

10 The oral LD50 of the sample stock solution to KM mice is more than 5000mg/kg body weight, and the sample stock solution is practically nontoxic according to the evaluation regulation of 2.3.1 acute oral toxicity test in disinfection technical Specification (2002 edition);

11 The micronucleus rate of the pleochromocyte of the mouse marrow polychromocyte micronucleus test has no obvious difference compared with a negative control group under three medicaments of 500mg/kg, 2000mg/kg and 5000mg/kg of the sample stock solution, and the liquid generated by the sample has no in-vivo chromosome damage effect on a KM mouse under three administration doses of 500mg/kg, 2000mg/kg and 5000mg/kg of the body weight according to the evaluation regulation of 2.3.8.4 mouse marrow polychromocyte micronucleus test in the technical specification for disinfection (2002 edition);

12 The highest integral mean value of each observation time point of the sample stock solution in a primary damage skin irritation test of New Zealand rabbits is 0, and the sample stock solution is graded according to the skin irritation strength of a skin irritation test of 2.3.3 in disinfection technical Specification (2002 edition), and is nonirritating;

13 The integral mean value of each animal per day of the sample stock solution for the complete skin irritation test of a new zealand rabbit for a plurality of times is 0, and the sample stock solution is graded according to the skin irritation strength of the skin irritation test of 2.3.3 in the technical Specification for Disinfection (2002 edition), and is nonirritating;

14 The sample stock solution has an average score of 0 for corneal damage, iris damage, conjunctival edema and conjunctival congestion of 3 animals subjected to the acute eye irritation test of New Zealand rabbits, and is non-irritant according to the eye irritation response grading standard of the acute eye irritation test of 2.3.4 in the Disinfection technical Specification (2002);

15 The stimulation index of the sample stock solution to the acute vaginal mucosa stimulation test (for multiple times) of New Zealand rabbits is 0, and the vaginal mucosa stimulation intensity is graded according to the vaginal mucosa stimulation test of 2.3.5 in the technical Specification for Disinfection (2002), so that the sample stock solution is nonirritating.

d) According to the analysis and detection of Wuhan virus institute, the medicine is characterized by having the following characteristics that the detection conclusion of the medicine for the new crown pneumonia virus SARS-COV-2 with the concentration of 200mg/L is as follows:

The second embodiment:

a) A wound aseptic liquid dressing for obstructing and inactivating SARS-COV-2 virus is characterized by comprising the following raw materials: 98.45wt% of hypochlorous acid water, 0.9 wt% of sodium chloride, 0.3 wt% of a stabilizer and 0.35 wt% of a chelating agent.

The hypochlorous acid water is non-electrolytic weakly acidic hypochlorous acid water, the concentration is 100 mg/L-120 mg/L, and the PH is 4.5-6.5; the sodium chloride is food grade; the stabilizer is sodium dihydrogen phosphate and is food grade; the chelating agent is ethylenediamine tetraacetic acid and is food grade or pharmaceutical grade.

and 3, step 3: preparing a sodium hypochlorite solution; preparing sodium hypochlorite into 8-9% sodium hypochlorite;

step 6: adding a chelating agent into the hypochlorous acid water in the step 5, and stirring for 28min;

and 7: adding the stabilizer into the hypochlorous acid water in the step 6, and stirring for 10min;

and step 8: adding sodium chloride into the hypochlorous acid water in the step 7, stirring for 6min, and standing for 3h;

c) The wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus is detected by microbiological research institute in Guangzhou city and is characterized by comprising the following characteristics:

1) The pH (25 ℃) of this sample was 5.89;

2) The arsenic content of the sample is less than 0.01mg/kg, the mercury content is less than 0.002mg/kg, and the lead content is less than 1.5mg/kg, so that the sample meets the requirements of technical safety standards of cosmetics (2015 edition);

3) The effective chlorine component of the sample is 105mg/L;

4) Placing the sample in a constant temperature box at 54 ℃ for 14 days (testing the validity period by one year), wherein the color has no obvious change, no sediment or suspended matters are generated, the content of effective components is 101.5mg/L after the sample is placed, the reduction rate of the effective components of the sample is 3.3 percent, the reduction rate of the effective components is less than or equal to 10 percent, and the validity period of the sample storage can be determined as one year;

5) The total bacterial colony number, the mould and yeast, the pseudomonas aeruginosa, the staphylococcus aureus and the beta hemolytic streptococcus detection result of the sample meet the requirements of GB27951-2011 'hygienic requirements for skin disinfectants';

6) The aseptic detection test result of the sample meets the requirements of GB27951-2011 hygienic requirement for skin disinfectants;

7) 1% lecithin, 3.0% Tween 80 and 1% sodium thiosulfate PBS solution are used as a neutralizer, the sample stock solution acts for 0.5min, the killing logarithm of staphylococcus aureus, escherichia coli and pseudomonas aeruginosa in suspension is more than 5, the killing rate is more than or equal to 99.999%, and the requirements of the disinfection technical specification (2002 edition) are met;

10 The sample stock solution has an oral LD50 of more than 5000mg/kg body weight for KM mice, and is practically nontoxic according to evaluation regulation of 2.3.1 acute oral toxicity test in disinfection technical Specification (2002 edition);

13 The integral mean value of each animal per day of the sample stock solution for a plurality of times of complete skin irritation tests of New Zealand rabbits is 0, and the sample stock solution is graded according to the skin irritation strength of a skin irritation test in disinfection technical Specification (2002 edition) 2.3.3, and is nonirritating;

16 Acute inhalation toxicity test on NIH mice, LD50 > 10573mg/m3, and toxicity was rated virtually nontoxic according to the Disinfection Specification (2002 edition).

d) According to the analysis and detection of Shanghai micro-spectrum chemical technology service company Limited, the method is characterized by comprising the following steps of: the inactivation logarithm of HIV virus is more than or equal to 4.02, and the antiviral activity rate is more than or equal to 99.99%; the inactivation logarithm of HPV16 virus is more than or equal to 4.04, and the antiviral activity rate is more than or equal to 99.99%.

Example three:

a) A wound sterile liquid dressing for obstructing and inactivating SARS-COV-2 virus is characterized in that the raw materials comprise: 98.23wt% of hypochlorous acid water, 0.88% of sodium chloride, 0.53% of a stabilizer and 0.36% of a chelating agent.

The hypochlorous acid water is non-electrolytic weakly acidic hypochlorous acid water, the concentration is 160 mg/L-180 mg/L, and the PH is 4.5-6.5; the sodium chloride is food grade; the stabilizer is in sodium hexametaphosphate and is food grade; the chelating agent is disodium ethylene diamine tetraacetate and is food grade or pharmaceutical grade.

b) The method for manufacturing the wound sterile liquid dressing for obstructing and inactivating the SARS-COV-2 virus is characterized by comprising the following steps:

and 6: adding a chelating agent into the hypochlorous acid water obtained in the step 5, and stirring for 9min;

and 7: adding the stabilizer into the hypochlorous acid water in the step 6, and stirring for 26min;

and 8: adding sodium chloride into the hypochlorous acid water in the step 7, stirring for 16min, and standing for 2.5h;

1) The pH (25 ℃) of this sample was 5.67;

3) 175mg/L of the effective chlorine component of the sample;

4) Placing the sample in a constant temperature box at 54 ℃ for 14 days (testing the validity period by one year), wherein the color has no obvious change, no sediment or suspended matters are generated, the content of the effective components is 163mg/L after the sample is placed, the reduction rate of the effective components of the sample is 6.9 percent, the reduction rate of the effective components is less than or equal to 10 percent, and the validity period of the sample storage can be determined as one year;

7) The stimulation index of the sample stock solution to the acute vaginal mucosa stimulation test (for multiple times) of New Zealand rabbits is 0, and the vaginal mucosa stimulation intensity is graded according to the vaginal mucosa stimulation test of the disinfection technical Specification (2002 edition), 2.3.5, and the sample stock solution is non-irritant.

7) 1% lecithin, 3.0% Tween 80 and 1% sodium thiosulfate in PBS solution are used as neutralizer, the sample stock solution acts for 0.5min, the killing logarithm of staphylococcus aureus and pseudomonas aeruginosa in suspension is more than 5, the killing rate is more than or equal to 99.999%, and the requirements of the disinfection technical specification (2002 edition) are met;

8) 1% lecithin, 3.0% Tween 80 and 1% sodium thiosulfate PBS solution are used as neutralizing agents, the sample stock solution acts for 0.5min, the killing logarithm of Candida albicans in suspension is more than 4, the killing rate is more than or equal to 99.99%, and the requirements of disinfection technical specification (2002 edition) are met;

d) According to the detection of the Joint-creation detection service company Limited in the department of Henan, the method is characterized by comprising the following steps:

1) The sterilization of the skin is carried out for 3min in a field test, the average killing logarithm of natural bacteria on the surface of the skin in each test is more than or equal to 1.00, and the requirements of sterilization technical specification (2002 edition) are met;

2) The mouse marrow pleochromocyte micronucleus test is negative, and meets the requirements of disinfection technical specification (2002 edition);

e) The detection of the comprehensive detection center of the research institute of science and technology according to the inspection and quarantine of China is characterized by having the following characteristics

1) The half lethal dose (LD 50) of the sample stock solution to ICR female and male mice is more than 5000mg/kg body weight, and the sample stock solution is practically nontoxic according to the evaluation regulation of 2.3.1 acute oral toxicity test in disinfection technical Specification (2002 edition);

2) The highest integral mean value of the sample stock solution to each observation time point of a plurality of times of complete skin irritation tests of the white rabbits with big ears in Japan is 0, and the sample stock solution is graded according to the skin irritation strength of the skin irritation test of 2.3.3 in the technical Specification for Disinfection (2002 edition), and is nonirritating;

3) The highest integral mean value of each observation time point of the sample stock solution in a primary damage skin irritation test of the white rabbit with big ear in Japan is 0, and the sample stock solution is graded according to the skin irritation strength of a skin irritation test of 2.3.3 in the technical Specification for Disinfection (2002 edition), and is nonirritating;

4) The highest integral mean value of the sample stock solution to each observation time point of an acute eye irritation test of a Japanese big-ear white rabbit is 0, and the sample stock solution is graded according to the skin irritation strength of a skin irritation test of 2.3.3 in the technical Specification for Disinfection (2002), and is nonirritating;

f) According to the analysis and detection of Wuhan virus institute, the medicine is characterized by having the following characteristics that the detection conclusion of the medicine for the new coronavirus SARS-COV-2 with the concentration of 200mg/L is as follows:

It should be noted that, although the present invention has been illustrated and described with reference to the foregoing embodiments, the technical solutions described in the foregoing embodiments may be modified or part of the technical features may be substituted equally, and any modification, part of equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims

1. A wound aseptic liquid dressing for obstructing and inactivating SARS-COV-2 virus is characterized by comprising the following raw materials: 97.3 to 98.84 weight percent of hypochlorous acid water, 0.86 to 0.90 percent of sodium chloride, 0.1 to 1.0 percent of stabilizer and 0.2 to 0.8 percent of chelating agent.

The hypochlorous acid water is non-electrolytic weakly acidic hypochlorous acid water, the concentration is 100 mg/L-200 mg/L, and the PH is 4.5-6.5; the sodium chloride is food grade; the stabilizer is one or a mixture of two of sodium dihydrogen phosphate and sodium hexametaphosphate, which are both food grade; the chelating agent is one or a mixture of ethylene diamine tetraacetic acid and disodium ethylene diamine tetraacetic acid, and is food grade or pharmaceutical grade.

2. The method for preparing the wound sterile liquid dressing for obstructing and inactivating the SARS-COV-2 virus of claim 1, which is characterized by comprising the following steps:

and 2, step: preparing a hydrochloric acid solution, namely preparing hydrochloric acid into 7.5-8.5% hydrochloric acid;

and 7: adding the stabilizer into the hypochlorous acid water in the step 6, and stirring for 5-20 min;

and step 8: adding sodium chloride into the hypochlorous acid water in the step 7, stirring for 5-20 min, and standing for 2-3h;