CN115676163A - Ultralow-temperature seed preservation device and method - Google Patents
Ultralow-temperature seed preservation device and method Download PDFInfo
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- CN115676163A CN115676163A CN202211707070.1A CN202211707070A CN115676163A CN 115676163 A CN115676163 A CN 115676163A CN 202211707070 A CN202211707070 A CN 202211707070A CN 115676163 A CN115676163 A CN 115676163A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention relates to the technical field of seed preservation, and discloses an ultralow-temperature seed preservation device and a preservation method, which comprise the following steps: s1, pretreating seeds before storage; s2, storing the seeds in a single cavity; the seeds are independently placed in a seed cavity for preservation; s3, preserving the seeds at ultralow temperature; the seed preservation temperature is controlled within the range of minus 10 ℃ to minus 20 ℃; s4, turning over seeds periodically; the posture of the seeds is adjusted through vibration, and the breathing and the vitality of the seeds are kept; and S5, releasing the seed dormancy by stepwise temperature rise. Carry out single chamber with the seed and preserve, avoid influencing each other between the seed and condense together, secondly, regularly or continuously stir the seed, prevent on the one hand that the seed from condensing at the inner wall in kind chamber, on the other hand adjusts the gesture of seed, avoids the inner wall influence of the partial region long-term contact kind chamber of seed to breathe freely, guarantees the required oxygen of seed respiration, keeps the seed vigor to improve the survival rate of seed under the ultra-low temperature preservation.
Description
Technical Field
The invention relates to the technical field of seed preservation, in particular to an ultralow-temperature seed preservation device and method.
Background
The life of normal seeds can be greatly prolonged by low-temperature preservation, which forms a consensus internationally, and a plurality of crop and wild plant seed banks are established based on the theory, the current germplasm resource preservation modes are three modes of normal temperature, low-temperature preservation and ultralow temperature, and the three preservation modes have the following environmental requirements:
1. short-term seed preservation (ambient temperature): the temperature of the seed bank is controlled at 15-20 ℃, the relative humidity is 50-60%, the water content of the seeds is below 12%, and the expected life of the seeds is 2-5 years.
2. Middle seed preservation (low temperature): the temperature of the seed bank is controlled within the range of 0-10 ℃, the relative humidity is not more than 60%, the water content of the seeds is 6-9%, and the expected service life of the seeds is 10-30 years.
3. The temperature of the seed cold storage is controlled in a range of-10 ℃ to-20 ℃ for a long time (ultralow temperature), and the relative humidity is not more than 60%. Internationally recommended reference standards for long-term storage of normal seeds are temperature conditions of-18 ℃ or less, seed moisture content of 5 + -1% (on a wet weight basis), expected life of seeds of 30-50 years, or longer.
Because the seed preservation temperature of the ultra-low temperature mode is lower, in addition to the influence of moisture in the environment, the moisture attached to the seeds can be frozen, the seeds can also generate moisture when absorbing the moisture for respiration, the seeds are basically accumulated and stored in a seed resource library or a seed box at present, so that the seeds are condensed together in the ultra-low temperature environment, when the preservation is finished and the post-treatment is carried out, the seeds are easy to mutually influence, the skin or the whole of the seeds are damaged, and the survival rate of the seed preservation is reduced; secondly, the seed at the in-process edge that the seed was preserved can condense at the box inner wall, leads to needing the separation seed when taking out the seed, causes the inconvenience that the seed was taken out, also damages the seed easily simultaneously, and the survival rate that the seed ultra-low temperature was preserved has been reduced to the indirectness, causes the influence to the use in later stage.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide an ultralow-temperature seed preservation device and a preservation method, seeds are preserved in a single cavity, so that the seeds are prevented from being influenced and coagulated together, and are turned over periodically or continuously, so that the seeds are prevented from being coagulated on the inner wall of the seed cavity, the seeds are prevented from being damaged when taken out, and the survival rate of the seeds under ultralow-temperature preservation is improved.
The purpose of the invention is realized by the following technical scheme: an ultra-low temperature seed preservation method comprises the following steps:
s1, pretreating seeds before storage;
s2, storing the seeds in a single cavity; the seeds are independently placed in a seed cavity for preservation;
s3, preserving seeds at ultralow temperature; the seed preservation temperature is controlled within the range of minus 10 ℃ to minus 20 ℃;
s4, turning over seeds periodically; the posture of the seeds is adjusted through vibration, and the breathing and the vitality of the seeds are kept;
and S5, releasing the seed dormancy by stepwise temperature rise.
The effect of adopting above-mentioned technical scheme does, carries out single chamber with the seed and preserves, avoids influencing each other between the seed and condenses together, secondly, regularly or continuously stirs the seed, prevents that the seed from condensing on the inner wall in kind chamber, guarantees can not damage the seed when taking out the seed, improves the survival rate of seed under the ultra-low temperature preservation.
In some embodiments, the step S1 further includes seed washing, seed plumpness screening, and seed drying in the shade.
The utility model provides a seed save set of ultra-low temperature, is including keeping the case, the lateral wall of keeping the case is provided with the vibration exciter, it stores the mechanism to be provided with single chamber in the case to keep, single chamber is stored the mechanism and is included the frame that deflects, the bottom plate and the storage board deflect, the frame that deflects rotate connect in the inner wall of keeping the case, the bottom plate that deflects rotate connect in the inner wall of frame deflects, be provided with on the bottom plate the storage board, wherein, the deflection axis perpendicular to of frame deflects the deflection axis of bottom plate, a plurality of seed chambers have been seted up on the storage board, the space in seed chamber can only hold a seed, the negative pressure chamber has been seted up in the bottom plate that deflects, a plurality of negative pressure holes have been seted up at the inner wall position in seed chamber to the storage board, the negative pressure hole with the negative pressure chamber communicates with each other.
Adopt above-mentioned technical scheme's effect does, directly place numerous seed on the reservoir plate, then the frame that deflects drives the reservoir plate and changes to the tilt state, make the seed produce motion or motion trend, the vibrations that the cooperation vibration exciter produced, make the seed move on the reservoir plate, make the automatic seed intracavity that rolls into of seed, because single seed can only be stored in the seed intracavity, realize the single chamber of seed and preserve, and the seed that falls into the seed intracavity is adsorbed to the setting in negative pressure hole, make the seed that falls into the seed intracavity can not break away from the seed intracavity because of vibrations, and the seed that does not fall into removes the one side of reservoir plate, the frame antiport that deflects this moment drives the reservoir plate reversal to the tilt state, make the seed from one side of reservoir plate to the opposite side motion, so relapse many times, the seed has all been placed in the seed intracavity of messenger's overwhelming number, thereby automatic single chamber to the seed is preserved, the intensity of manual labor has been reduced, and the efficiency of placing of seed has been improved.
In some embodiments, the lateral wall of storage board is provided with spacing curb plate around being provided with, spacing curb plate is located the top of storage board, the height of spacing curb plate is less than the size of seed, the top of storage board is provided with shelters from the mechanism, shelter from the mechanism including removing the area that shelters from that sets up, shelter from the area and be used for sheltering from when vibrations turn the seed on the storage board.
In some embodiments, shelter from mechanism still includes drive roll axle, driven roller axle and small motor, be provided with respectively on two relative lateral walls of frame that deflects and remove support and fixed bolster, drive roll axle and driven roller axle rotate respectively to be connected on fixed bolster and the removal support, the small motor is installed on the fixed bolster, the output shaft of small motor with the one end transmission of drive roll axle is connected, it is annular to shelter from the area, it is in to shelter from the area transmission suit on drive roll axle and driven roller axle, the frame that deflects is located shelter from the inner ring in area, shelter from taking to have seted up the rectangle and lead to the groove, the size that the rectangle leads to the groove with the storage plate size is the same, just the rectangle leads to the groove can move directly over the storage plate.
In some embodiments, a rectangular groove is formed in a side wall of the deflection outer frame provided with the moving support, a tensioning column is slidably arranged in the rectangular groove, one end, far away from the deflection bottom plate, of the tensioning column is connected with the moving support, a lead screw is rotatably arranged in the rectangular groove, the tensioning column is in threaded connection with the lead screw, and one end, far away from the tensioning column, of the lead screw penetrates through the fixed support and is connected with a screw cap.
In some embodiments, reinforcing rods are arranged on both sides of the axis of the screw rod, each reinforcing rod comprises a fixed rod and a sliding rod, one end of each fixed rod is fixedly connected with the deflection bottom plate, the other end of each fixed rod is slidably penetrated with the corresponding sliding rod, one end of each sliding rod, far away from the corresponding fixed rod, is connected with the corresponding movable support, and a fastening bolt is connected to the side wall of each fixed rod in a threaded manner and penetrates into the corresponding fixed rod to be abutted against the corresponding sliding rod.
In some embodiments, the storage plate is of a thin plate structure, the storage plate is formed by a mode of being sunken downwards and protruding to form the seed cavity, a negative pressure groove is formed in the deflection bottom plate corresponding to the seed cavity, an elastic layer wraps the inner wall of the negative pressure groove, a column body of the seed cavity is located in the negative pressure groove and is in transition fit with the elastic layer, a through hole is formed in the bottom of the negative pressure groove and communicated with the negative pressure cavity, and a negative pressure pump is arranged on the storage box and communicated with the negative pressure cavity through a negative pressure pipe.
In some embodiments, a deflection shaft is fixed to a side wall of the deflection bottom plate, a torsion spring is sleeved on the deflection shaft, and the deflection shaft is rotatably connected with the deflection outer frame through the torsion spring.
In some embodiments, the mechanism is stored to single chamber is provided with a plurality ofly, and is a plurality of the mechanism is stored to single chamber sets up from last interval down, the lateral wall that deflects the frame is fixed with the driven shaft, the driven shaft pass through the bearing with save the case and rotate and be connected, the outer wall of saving the case is fixed with the drive case, the driven shaft extends to drive incasement and be provided with first gear, adjacent two meshing has intermediate gear between the first gear, the drive incasement internal rotation is provided with the worm, the one end transmission of worm is connected with the motor, the drive incasement internal rotation is provided with the transmission shaft, key connection has the worm wheel on the transmission shaft, the worm wheel with the worm meshing, key connection has the second gear on the transmission shaft, the second gear with the top or the below first gear meshing.
The invention has the beneficial effects that:
1. carry out single chamber with the seed and preserve, avoid influencing each other between the seed and condense together, secondly, regularly or continuously stir the seed, prevent that the seed from condensing on the inner wall in kind chamber, guarantee can not damage the seed when taking out the seed, improve the survival rate of seed under the ultra-low temperature preservation.
2. Directly place numerous seeds on the reservoir plate, then the frame that deflects drives the reservoir plate and changes to the tilt state, make the seed produce motion or motion trend, the vibrations that the cooperation vibration exciter produced, make the seed move on the reservoir plate, make the automatic seed intracavity that rolls into of seed, because single seed can only be stored to the seed intracavity, realize the single chamber of seed and preserve, and the seed that the setting in negative pressure hole will fall into the seed intracavity adsorbs, make the seed that falls into the seed intracavity break away from the seed intracavity because of vibrations, and the seed that does not fall into removes the one side of reservoir plate, the frame antiport drives the reservoir plate reversal to the tilt state this moment, make the seed from the one side of reservoir plate to the opposite side motion, so repeat many times, the seed has all been placed to the seed intracavity of making the overwhelming majority, thereby automatically, carry out single chamber to the seed and preserve, the artifical intensity of labour has been reduced, and the efficiency of placing of seed has been improved.
3. Regularly or continuously turn the seed, prevent that the seed from condensing on the inner wall in kind chamber, when turning the seed, shelter from the area and move to the storage plate directly over after, start the vibration exciter, make the save case produce vibrations through the vibration exciter, and then make single chamber storage mechanism produce vibrations, make the seed beat in the seed intracavity, make the seed turn, prevent on the one hand that the seed from condensing the inner wall in kind chamber, on the other hand adjusts the gesture of seed, avoid the inner wall influence ventilative of the partial region long-term contact kind chamber of seed, guarantee that the seed breathes required oxygen, keep the seed vigor, owing to shelter from the size that highly is less than the seed between area and the storage plate, guarantee when the seed is turned in vibrations, the seed can not break away from in the seed intracavity.
Drawings
FIG. 1 is a flow chart of a method of seed preservation at ultra-low temperatures in accordance with the present invention;
FIG. 2 is a partial schematic view of a single-compartment storage mechanism of an ultra-low temperature seed storage device of the present invention;
FIG. 3 is a top plan view of a single-chamber storage mechanism of an ultra-low temperature seed storage device of the present invention;
FIG. 4 isbase:Sub>A sectional view taken along line A-A of FIG. 3;
FIG. 5 is an enlarged view taken at A in FIG. 4;
FIG. 6 is a view of the single chamber storage mechanism of the present invention with seeds deposited;
FIG. 7 is a state diagram of the single-chamber storage mechanism of the present invention during the turning of seeds;
FIG. 8 is a schematic view of the internal construction of an ultra-low temperature seed holding apparatus of the present invention;
FIG. 9 is a schematic external view of an ultra-low temperature seed holding apparatus of the present invention;
in the figure, 1-holding tank, 2-vibration exciter, 3-deflection outer frame, 4-deflection bottom plate, 5-storage plate, 6-seed chamber, 7-negative pressure chamber, 8-negative pressure hole, 9-limit side plate, 10-blocking belt, 11-driving roll shaft, 12-driven roll shaft, 13-small motor, 14-moving support, 15-fixed support, 16-rectangular groove, 17-tension column, 18-lead screw, 19-turncap, 20-fixed rod, 21-sliding rod, 22-fastening bolt, 24-negative pressure groove, 25-through hole, 26-negative pressure pump, 27-negative pressure pipe, 28-deflection shaft, 29-driven shaft, 30-driving tank, 31-first gear, 32-intermediate gear, 33-worm, 34-motor, 35-worm gear, 36-driving shaft, 37-second gear, 38-rectangular through groove.
Detailed Description
The technical solutions of the present invention are further described in detail below with reference to the accompanying drawings, but the scope of the present invention is not limited to the following.
As shown in fig. 1, a method for preserving seeds at ultra-low temperature comprises the following steps:
s1, pretreating seeds before storage;
s2, storing the seeds in a single cavity; the seeds are independently placed in the seed cavity 6 for storage, the seeds are stored in a single cavity, mutual influence and condensation between the seeds are avoided, the air permeability of each seed is ensured, and the activity of the seeds is not influenced.
S3, preserving the seeds at ultralow temperature; the seed preservation temperature is controlled within the range of minus 10 ℃ to minus 20 ℃;
s4, turning over seeds periodically; the posture of the seeds is adjusted through vibration, the breathing and the vitality of the seeds are kept, on one hand, the seeds are prevented from being condensed on the inner wall of the seed cavity 6, on the other hand, the posture of the seeds is adjusted, the influence of long-term contact of partial regions of the seeds with the inner wall of the seed cavity 6 on ventilation is avoided, oxygen required by the breathing of the seeds is ensured, and the vitality of the seeds is kept;
and S5, releasing the seed dormancy by stepwise temperature rise.
The storage method aims to: carry out single chamber with the seed and preserve, avoid influencing each other between the seed and condense together, secondly, regularly or continuously stir the seed, prevent that the seed from condensing on the inner wall of seed chamber 6, guarantee can not damage the seed when taking out the seed, improve the survival rate of seed under the ultra-low temperature preservation.
In some embodiments, the step S1 further includes the steps of washing the seeds, screening the plumpness of the seeds, drying the seeds in the shade, etc., so that the preserved seeds have higher quality and the mortality rate of the preserved seeds is reduced.
As shown in fig. 2 to 9, an ultra-low temperature seed storage device includes a storage box 1, a vibration exciter 2 is disposed on an outer side wall of the storage box 1, a single-cavity storage mechanism is disposed in the storage box 1, the single-cavity storage mechanism includes a deflection outer frame 3, a deflection bottom plate 4 and a storage plate 5, the deflection outer frame 3 is rotatably connected to an inner wall of the storage box 1, a deflection shaft 28 is fixed to a side wall of the deflection bottom plate 4, a torsion spring is sleeved on the deflection shaft 28, the deflection shaft 28 is rotatably connected to the deflection outer frame 3 through the torsion spring, the storage plate 5 is disposed on the deflection bottom plate 4, wherein a deflection axis of the deflection outer frame 3 is perpendicular to a deflection axis of the deflection bottom plate 4, a plurality of seed cavities 6 are disposed on the storage plate 5, a space of the seed cavities 6 can only contain one seed, a negative pressure cavity 7 is disposed in the deflection bottom plate 4, a plurality of negative pressure holes 8 are disposed on an inner wall of the seed cavities 6 of the storage plate 5, and the negative pressure holes 8 are communicated with the negative pressure cavities 7; a plurality of seeds are directly placed on the storage plate 5, then the deflection outer frame 3 drives the storage plate 5 to be in an inclined state, so that the seeds generate movement or movement trend, the seeds move on the storage plate 5 by matching with the vibration generated by the vibration exciter 2, so that the seeds automatically roll into the seed cavities 6, and because only a single seed can be stored in the seed cavities 6, the single-cavity storage of the seeds is realized, the seeds falling into the seed cavities 6 are adsorbed by the arrangement of the negative pressure holes 8, so that the seeds falling into the seed cavities 6 cannot be separated from the seed cavities 6 due to the vibration, and the seeds not falling into the seed cavities move to one side of the storage plate 5, at the moment, the deflection outer frame 3 rotates reversely to drive the storage plate 5 to be in an inclined state, so that the seeds move from one side of the storage plate 5 to the other side, and the operation is repeated for a plurality of times, so that the seeds are all placed in the seed cavities 6, the seeds are automatically subjected to the single-cavity storage, the labor intensity is reduced, and the seed placement efficiency is improved; because the deflection bottom plate 4 is rotationally connected with the deflection outer frame 3 through the torsion spring, when the vibration exciter 2 does not work, the deflection bottom plate 4 is in a horizontal state under the action of the torsion spring, when the vibration exciter 2 works to ensure that the storage box 1 generates vibration, the torsion spring is bent due to vibration, so that the deflection bottom plate 4 generates vibration, the deflection direction of the deflection bottom plate 4 is perpendicular to the deflection direction of the deflection outer frame 3, thereby breaking the directional sliding of seeds and matching the vibration effect, so that the seeds move in multiple directions on the storage plate 5, more seed cavities 6 on the storage plate 5 can fall into the seeds, and the quantity of the seeds stored can meet the requirement.
Further, as shown in fig. 3 to 5, the storage plate 5 is a thin plate structure, the storage plate 5 adopts a downward concave and protruding form to form a seed cavity 6, a negative pressure groove 24 is formed on the deflection bottom plate 4 corresponding to the seed cavity 6, an elastic layer is wrapped on the inner wall of the negative pressure groove 24, a cylinder of the seed cavity 6 is positioned in the negative pressure groove 24 and is in transition fit with the elastic layer, a gap between the cylinder of the seed cavity 6 and the negative pressure groove 24 is sealed through the arrangement of the elastic layer, so that the negative pressure environment in the negative pressure groove 24 is not affected, a through hole 25 is formed at the bottom of the negative pressure groove 24, the through hole 25 is communicated with the negative pressure cavity 7, as shown in fig. 8, a negative pressure pump 26 is arranged on the storage box 1, the negative pressure pump 26 is communicated with the negative pressure cavity 7 through a negative pressure pipe 27, negative pressure is generated in the negative pressure groove 24 through the negative pressure pump 26, the negative pressure is transmitted into the seed cavity 6 through the arrangement of the through hole 25 and the negative pressure hole 8, and then, the storage plate 5 is of a detachable structure, so that the storage plate 5 matched with the size of the seed can be conveniently replaced according to the seed cavity 5, and can accommodate only one seed.
Further, as shown in fig. 8, a plurality of single-chamber storage mechanisms are provided, the plurality of single-chamber storage mechanisms are arranged at intervals from top to bottom, a driven shaft 29 is fixed on the side wall of the deflection outer frame 3, the driven shaft 29 is rotatably connected with the storage box 1 through a bearing, a driving box 30 is fixed on the outer wall of the storage box 1, the driven shaft 29 extends into the driving box 30 and is provided with a first gear 31, an intermediate gear 32 is engaged between two adjacent first gears 31, a worm 33 is rotatably arranged in the driving box 30, one end of the worm 33 is in transmission connection with a motor 34, a transmission shaft 36 is rotatably arranged in the driving box 30, a worm wheel 35 is in key connection with the transmission shaft 36, the worm wheel 35 is engaged with the worm 33, a second gear 37 is in key connection with the transmission shaft 36, the second gear 37 is engaged with the uppermost or lowermost first gear 31, the number of seeds is increased by the plurality of single-chamber storage mechanisms, because the single chamber stores the mechanism and is provided with a plurality ofly, consequently, it is very necessary to place the seed in seed chamber 6 through automatic mode, a plurality of single chamber store the mechanism and can the synchronous operation, the mode of placing the seed for the manual work has improved the efficiency of placing of seed greatly, specifically speaking, drive worm 33 through motor 34 and rotate, drive transmission shaft 36 through the meshing of worm wheel 35 with worm 33 and rotate, transmission shaft 36 through second gear 37 with one of them the meshing of first gear 31 drives driven shaft 29 and rotates, through the setting of intermediate gear 32, make the rotation direction between a plurality of deflection frames 3 unanimous, and then drive a plurality of deflection frames 3 synchronous deflection, after the seed is placed and is accomplished, deflection frame 3 changes the horizontal position and carries out the ultra-low temperature preservation of seed.
In some embodiments, as shown in fig. 1, 6 and 7, a position-limiting side plate 9 is disposed around a side wall of the storage plate 5, the position-limiting side plate 9 is located above the storage plate 5, the height of the position-limiting side plate 9 is smaller than the size of the seeds, a shielding mechanism is disposed above the storage plate 5, the shielding mechanism comprises a movably disposed shielding belt 10, the shielding belt 10 is used for shielding the storage plate 5 when the seeds are flipped through vibration, the shielding mechanism further comprises a driving roller shaft 11, a driven roller shaft 12 and a small motor 13, a movable bracket 14 and a fixed bracket 15 are disposed on two opposite side walls of the deflection outer frame 3, the driving roller shaft 11 and the driven roller shaft 12 are rotatably connected to the fixed bracket 15 and the movable bracket 14, the small motor 13 is mounted on the fixed bracket 15, an output shaft of the small motor 13 is in transmission connection with one end of the driving roller shaft 11, the shielding belt 10 is annular, the shielding belt 10 is sleeved on the driving roller shaft 11 and the driven roller shaft 12 in a transmission manner, the deflection outer frame 3 is positioned on the inner ring of the shielding belt 10, the shielding belt 10 is provided with a rectangular through groove 38, the size of the rectangular through groove 38 is the same as that of the storage plate 5, the rectangular through groove 38 can run right above the storage plate 5, the driving roller shaft 11 is driven to rotate by a small motor 13, the shielding belt 10 is driven to run by matching with the arrangement of the driven roller shaft 12, switching between seed placement and seed turning is realized, specifically, when seeds are placed, the shielding belt 10 runs to the rectangular through groove 38 and is positioned right above the storage plate 5, the situation that the shielding belt 10 blocks the movement of the seeds is avoided, so that the seeds can smoothly fall into the seed cavity 6, and the arrangement of the limiting side plate 9 effectively avoids the situation that the seeds fall out from the side edge of the storage plate; when the seeds are turned, the rectangular through groove 38 of the shielding belt 10 is positioned below the storage plate 5, so that the complete surface of the shielding belt 10 is in contact with the limiting side plate 9 to cover the storage plate 5, the vibration exciter 2 is started, the storage box 1 is vibrated through the vibration exciter 2, and then the single-cavity storage mechanism is vibrated, so that the seeds jump in the seed cavity 6, and the seeds are turned, on one hand, the seeds are prevented from being condensed on the inner wall of the seed cavity 6, on the other hand, the posture of the seeds is adjusted, the phenomenon that partial regions of the seeds are in long-term contact with the inner wall of the seed cavity 6 to affect ventilation is avoided, oxygen required by seed respiration is ensured, and the vitality of the seeds is kept, and as the height between the shielding belt 10 and the storage plate 5 is smaller than the size of the seeds, the seeds are ensured not to be separated from the seed cavity 6 when the seeds are turned in a vibration mode.
Further, as shown in fig. 1, a rectangular groove 16 is formed in a side wall of the deflection outer frame 3 where the moving bracket 14 is disposed, a tensioning column 17 is slidably disposed in the rectangular groove 16, one end of the tensioning column 17, which is far away from the deflection bottom plate 4, is connected to the moving bracket 14, a lead screw 18 is rotatably disposed in the rectangular groove 16, the tensioning column 17 is threadedly connected to the lead screw 18, one end of the lead screw 18, which is far away from the tensioning column 17, passes through the fixed bracket 15 and is connected to a screw cap 19, the lead screw 18 is mounted by avoiding a deflection shaft 28, and the lead screw 18 is located below the deflection bottom plate 4, so as to avoid interference with the lead screw 18 when the deflection bottom plate 4 deflects, and then a gap of the rotating screw cap 19 is left between the driving roller shaft 11 and the fixed bracket 15, so that the screw cap 19 is conveniently rotated by using a tool to adjust the position of the moving bracket 14, the distance between the moving bracket 14 and the fixed bracket 15 is adjustable, so that the shielding belt 10 is conveniently mounted on the driving roller shaft 11 and the driven roller shaft 12, after the shielding belt 10 is mounted, the shielding belt 10 is in a tensioned state, and the shielding belt 10 can smoothly runs, and the distance between the rotating nut 18 is adjusted, and the tensioning column is adjusted by the tensioning column 17, so that the tensioning column 17, the tensioning column is adjusted by the tensioning column 17, and the tensioning column is connected to enable the tensioning column to move freely along the tensioning column to move along the tensioning column 17, and the tensioning column to enable the tensioning column to move freely; both sides of 18 axes of lead screw all are provided with the stiffener, the stiffener includes dead lever 20 and slide bar 21, the one end and the deflection bottom plate 4 fixed connection of dead lever 20, slide bar 21 is worn to be equipped with in the other end slides, the one end that slide bar 21 kept away from dead lever 20 is connected with removal support 14, the lateral wall threaded connection of dead lever 20 has fastening bolt 22, fastening bolt 22 penetrates in dead lever 20 and abuts with slide bar 21, improve the joint strength who removes support 14 through setting up of stiffener, when adjusting the interval between removal support 14 and the fixed bolster 15, need unscrew fastening bolt 22 earlier, make slide bar 21 slide in dead lever 20, prevent to produce with the removal of tensioning post 17 and interfere, after the regulation is accomplished, it can to screw up fastening bolt 22.
In the description of the present invention, it is to be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "one side", "top", "inner", "front", "center", "two ends", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention; and those skilled in the art will appreciate that the benefits to be achieved by the present invention are only better than those achieved by current embodiments of the prior art under certain circumstances, rather than the best use directly in the industry.
The foregoing is illustrative of the preferred embodiments of the present invention, and it is to be understood that the invention is not limited to the precise form disclosed herein and is not to be construed as limited to the exclusion of other embodiments, and that various other combinations, modifications, and environments may be used and modifications may be made within the scope of the concepts described herein, either by the above teachings or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. An ultra-low temperature seed preservation method is characterized by comprising the following steps:
s1, pretreating seeds before storage;
s2, storing the seeds in a single cavity; seeds are independently placed in the seed cavity (6) for preservation;
s3, preserving the seeds at ultralow temperature; the preservation temperature of the seeds is controlled within the range of minus 10 ℃ to minus 20 ℃;
s4, turning over seeds periodically; the posture of the seeds is adjusted through vibration, and the breathing and the vitality of the seeds are kept;
and S5, releasing the seed dormancy by stepwise temperature rise.
2. The method of claim 1, further comprising the steps of washing seeds, selecting the seed plumpness, and drying the seeds in the shade in step S1.
3. The utility model provides a seed save set of ultra-low temperature, is including keeping case (1), its characterized in that, the lateral wall of keeping case (1) is provided with vibration exciter (2), it stores the mechanism to be provided with single chamber in keeping case (1), single chamber is stored the mechanism including deflecting frame (3), deflection bottom plate (4) and reservoir plate (5), deflection frame (3) rotate connect in the inner wall of keeping case (1), deflection bottom plate (4) rotate connect in the inner wall of deflection frame (3), be provided with on deflection bottom plate (4) storage plate (5), wherein, the deflection axis perpendicular to of deflection frame (3) the deflection axis of deflection bottom plate (4), a plurality of seed chamber (6) have been seted up on storage plate (5), the space in seed chamber (6) can only hold a seed, seted up in deflection bottom plate (4) negative pressure chamber (7), a plurality of negative pressure holes (8) have been seted up in the inner wall position in seed chamber (6) to storage plate (5), negative pressure hole (8) with negative pressure chamber (7) communicate with each other.
4. An ultra-low temperature seed preservation device as claimed in claim 3, characterized in that, the side wall of storage plate (5) is provided with spacing curb plate (9) around, spacing curb plate (9) are located the top of storage plate (5), the height of spacing curb plate (9) is less than the size of seed, the top of storage plate (5) is provided with shelters from the mechanism, shelter from the mechanism including the sheltering from area (10) that the removal set up, sheltering from area (10) are used for covering when the seed is turned in vibrations on storage plate (5).
5. The ultra-low temperature seed storage device as claimed in claim 4, wherein the shielding mechanism further comprises a driving roller shaft (11), a driven roller shaft (12) and a small motor (13), two opposite side walls of the deflection frame (3) are respectively provided with a movable support (14) and a fixed support (15), the driving roller shaft (11) and the driven roller shaft (12) are respectively rotatably connected to the fixed support (15) and the movable support (14), the small motor (13) is installed on the fixed support (15), an output shaft of the small motor (13) is connected with one end of the driving roller shaft (11) in a transmission manner, the shielding belt (10) is annular, the shielding belt (10) is sleeved on the driving roller shaft (11) and the driven roller shaft (12) in a transmission manner, the deflection frame (3) is located in an inner ring of the shielding belt (10), a rectangular through groove (38) is formed in the shielding belt (10), the size of the rectangular through groove (38) is the same as that of the storage plate (5) runs above the rectangular through groove (38).
6. An ultra-low temperature seed preservation device as claimed in claim 5, characterized in that the side wall of the deflection outer frame (3) provided with the moving bracket (14) is provided with a rectangular groove (16), a tensioning column (17) is slidably arranged in the rectangular groove (16), one end of the tensioning column (17) far away from the deflection bottom plate (4) is connected with the moving bracket (14), a lead screw (18) is rotatably arranged in the rectangular groove (16), the tensioning column (17) is in threaded connection with the lead screw (18), and one end of the lead screw (18) far away from the tensioning column (17) passes through the fixed bracket (15) and is connected with a screw cap (19).
7. An ultra-low temperature seed preservation device as claimed in claim 6, characterized in that, the two sides of the axis of the lead screw (18) are provided with reinforcing rods, the reinforcing rods comprise a fixed rod (20) and a sliding rod (21), one end of the fixed rod (20) is fixedly connected with the deflection bottom plate (4), the other end is slidably penetrated with the sliding rod (21), one end of the sliding rod (21) far away from the fixed rod (20) is connected with the movable bracket (14), the side wall of the fixed rod (20) is connected with a fastening bolt (22) by screw thread, and the fastening bolt (22) penetrates into the fixed rod (20) and abuts against the sliding rod (21).
8. The ultra-low temperature seed preservation device of claim 3, characterized in that, the storage board (5) is the sheet structure, the storage board (5) adopts the outstanding form formation of undercut the seed chamber (6), it corresponds on bottom plate (4) to deflect seed chamber (6) has seted up negative pressure recess (24), the inner wall parcel elastic layer of negative pressure recess (24), the cylinder of seed chamber (6) is located in negative pressure recess (24) and with elastic layer transition adaptation, through-hole (25) have been seted up to the bottom of negative pressure recess (24), through-hole (25) with negative pressure chamber (7) communicate with each other, be provided with negative pressure pump (26) on the storage case (1), negative pressure pump (26) communicate through negative pressure pipe (27) negative pressure chamber (7).
9. An ultra-low temperature seed preservation device as claimed in claim 3, characterized in that the side wall of the deflection bottom plate (4) is fixed with a deflection shaft (28), the deflection shaft (28) is sleeved with a torsion spring, and the deflection shaft (28) is rotatably connected with the deflection outer frame (3) through the torsion spring.
10. The ultra-low temperature seed preservation device of claim 9, characterized in that, the mechanism is stored to single chamber is provided with a plurality ofly, and is a plurality of the mechanism is stored to single chamber sets up from last interval down, the lateral wall that deflects frame (3) is fixed with driven shaft (29), driven shaft (29) through the bearing with it rotates to connect to keep case (1), the outer wall of keeping case (1) is fixed with drive case (30), driven shaft (29) extend to in drive case (30) and be provided with first gear (31), adjacent two the meshing has intermediate gear (32) between first gear (31), the internal rotation of drive case (30) is provided with worm (33), the one end transmission of worm (33) is connected with motor (34), the internal rotation of drive case (30) is provided with transmission shaft (36), key connection has worm wheel (35) on transmission shaft (36), worm wheel (35) with worm (33) meshing, key connection has second gear (37) on transmission shaft (36), second gear (37) and the top or the first gear (31) below mesh.
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