CN115672227A - Many liquid drops operating means based on surface acoustic wave - Google Patents
Many liquid drops operating means based on surface acoustic wave Download PDFInfo
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- CN115672227A CN115672227A CN202211343734.0A CN202211343734A CN115672227A CN 115672227 A CN115672227 A CN 115672227A CN 202211343734 A CN202211343734 A CN 202211343734A CN 115672227 A CN115672227 A CN 115672227A
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- surface acoustic
- interdigital electrodes
- acoustic wave
- lithium niobate
- niobate substrate
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Abstract
The invention discloses a multi-droplet operation device based on surface acoustic waves, which comprises a lithium niobate substrate, N interdigital electrodes, an operation panel, vibration absorption glue and a control module; the operation plate is arranged in the center of the lithium niobate substrate, and the N interdigital electrodes are uniformly arranged around the circumference of the operation plate; a planning path for liquid drop driving and liquid drop reaction is arranged on the operation board; the control module is electrically connected with the N interdigital electrodes and is used for controlling the N interdigital electrodes to emit corresponding liquid drops on the surface acoustic wave driving operation panel to move on a planned path. The invention can realize the accurate driving, merging and splitting control of multiple liquid drops, the operating panel can be replaced, and the economical efficiency is good.
Description
Technical Field
The invention relates to the technical field of micro-droplet operation, in particular to a multi-droplet operation device based on surface acoustic waves.
Background
The traditional biological detection and biological reaction are carried out in a test tube, and the defects of long mixing reaction time, high mixing uniformity, difficult bubble discharge and the like exist. The specific surface of the droplets is large, the reaction between the droplets is rapid, and the uniformity of the composition is also easily improved. The reaction on a two-dimensional operation board platform can easily realize the removal of bubbles in liquid drops, the stirring of the liquid drops to make components uniform and the adsorption of the liquid drops.
The electric field or the magnetic field drives the liquid drop to require that the liquid drop has electric charge or magnetism, thereby greatly restricting the application of the two-dimensional micro-reaction platform. The surface acoustic wave is an elastic wave, has no charge or magnetism requirement on the liquid drop, and has an advantage in the aspect of liquid drop driving. At present, the liquid drop driven on the solid surface by utilizing the surface acoustic wave has the defects of difficulty in driving the liquid drop to accurately move, difficulty in driving a plurality of liquid drops to react sequentially and the like. Based on the interdigital transducer array of the surface acoustic wave, the free movement of liquid drops on the surface of the fluorinated oil can be realized by utilizing the acoustic flow effect, but the problems that the liquid drops generated after the reaction are difficult to extract from the fluorinated oil and the chemical properties of the fluorinated oil limit biological experiments exist.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a multiple-droplet operation device based on surface acoustic waves, aiming at the defects related in the background technology, so as to realize the precise driving, merging and splitting of multiple droplets, and the operation board is adhered on a substrate, can be replaced, has good economy and can realize large-scale application.
The invention adopts the following technical scheme for solving the technical problems:
a multi-droplet operation device based on surface acoustic waves comprises a lithium niobate substrate, N interdigital electrodes, an operation plate, vibration absorption glue and a control module, wherein N is a natural number more than or equal to 3;
the N interdigital electrodes and the operation plate are arranged on the lithium niobate substrate, wherein the operation plate is arranged in the center of the lithium niobate substrate, and the N interdigital electrodes are uniformly arranged around the circumference of the operation plate;
a planned path for liquid drop driving and liquid drop reaction is arranged on the operating board; the planning path comprises a plurality of stagnation points and a plurality of connecting grooves for communicating the stagnation points, wherein the stagnation points are ridge microstructures for forming a wetting gradient and are used for adsorbing liquid drops;
the control module is electrically connected with the N interdigital electrodes respectively and is used for controlling the N interdigital electrodes to emit corresponding liquid drops on the surface acoustic wave driving operation panel to move on a planned path, so that the driving, merging and splitting of multiple liquid drops are realized;
the vibration absorption glue is arranged on the lithium niobate substrate to form a closed loop including the lithium niobate substrate and the N interdigital electrodes and used for absorbing overflowing surface acoustic waves.
The surface acoustic wave-based multi-drop operation device further comprises N reflecting gratings, wherein the N reflecting gratings are correspondingly arranged on the outer sides of the N interdigital electrodes one by one and are all positioned in a closed loop formed by vibration absorption glue, and the N reflecting gratings are used for enhancing the driving force of the surface acoustic wave to liquid on an operation plate.
As a further optimization scheme of the multi-droplet operation device based on the surface acoustic wave, the control module comprises a signal source and a power amplifier, the signal source is used for generating a sinusoidal signal, and the signal is amplified by the power amplifier and then acts on the interdigital transducer to generate the surface acoustic wave.
As a further optimization scheme of the acoustic surface wave-based multi-droplet operation device, the operation plate is attached to the lithium niobate substrate through glue.
As a further optimization scheme of the multi-drop operation device based on the surface acoustic wave, N is 4, and the thickness range of the interdigital electrode is 100-200 nm.
Compared with the prior art, the invention adopting the technical scheme has the following technical effects:
1. the invention realizes the accurate driving of the liquid drop, and simultaneously, the liquid drop in a certain size span range can be accurately positioned; the stagnation points of different longitudinal rows are only communicated with each other through infiltration gradients, so that the ordered driving of multiple liquid drops is realized; when the liquid drop passes through a specific microstructure, the liquid drop is split under the combined action of the infiltration gradient, the capillary effect and the surface acoustic wave;
2. the operation plate is adhered to the lithium niobate substrate and can be torn off and replaced, so that the maintenance cost is greatly reduced; the operation panel is a two-dimensional platform, and has the advantages of strong operability, easiness in integrating other equipment and the like;
3. the device has simple and portable structure and strong operability, and can realize non-contact accurate driving of multiple liquid drops and merging and splitting of the liquid drops; the sample loss is less, the reaction time is short, and the method can be applied to various biochemical micro-droplet operations.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of the electric fields between the motors in the interdigitated electrodes of the present invention;
FIG. 3 is a schematic view of the structure of the operation panel of the present invention.
In the figure, 1 is an operation plate, 2 is an interdigital electrode, 3 is a reflecting grid, 4 is a lithium niobate substrate, and 5 is vibration absorption glue.
Detailed Description
The technical scheme of the invention is further explained in detail by combining the attached drawings:
the present invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. In the drawings, components are exaggerated for clarity.
As shown in fig. 1, the invention discloses a multiple-droplet operation device based on surface acoustic waves, which comprises a lithium niobate substrate, N interdigital electrodes, an operation plate, a vibration absorption glue and a control module, wherein N is a natural number greater than or equal to 3;
the N interdigital electrodes and the operation plate are arranged on the lithium niobate substrate, wherein the operation plate is arranged at the center of the lithium niobate substrate, and the N interdigital electrodes are uniformly arranged around the circumference of the operation plate; as shown in fig. 2, alternating electric fields are generated between the electrodes of the interdigital electrode, and surface acoustic waves are excited on the surface of the lithium niobate wafer according to the inverse piezoelectric effect;
as shown in fig. 3, a planned path for droplet driving and droplet reaction is provided on the operation panel; the planning path comprises a plurality of stagnation points and a plurality of connecting grooves for communicating the stagnation points, wherein the stagnation points are ridge microstructures forming a wetting gradient and used for adsorbing liquid drops;
the control module is electrically connected with the N interdigital electrodes respectively and is used for controlling the N interdigital electrodes to emit corresponding liquid drops on the surface acoustic wave driving operation panel to move on a planned path, so that the driving, merging and splitting of multiple liquid drops are realized;
the vibration absorption glue is arranged on the lithium niobate substrate to form a closed loop including the lithium niobate substrate and the N interdigital electrodes and used for absorbing overflowing surface acoustic waves.
The invention can also comprise N reflecting gratings which are correspondingly arranged at the outer sides of the N interdigital electrodes one by one and are all positioned in a closed loop formed by vibration absorption glue, and the N reflecting gratings are used for enhancing the driving force of the surface acoustic wave to the liquid on the operation plate.
The control module comprises a signal source and a power amplifier, wherein the signal source is used for generating a sinusoidal signal, and the signal is amplified by the power amplifier and then acts on the interdigital transducer to generate surface acoustic waves.
The operation plate is adhered to the lithium niobate substrate through gluing, N is preferably 4, and the thickness of the interdigital electrode is in the range of 100-200 nm.
The acoustic surface waves excited by the interdigital transducer can be coupled into standing waves and traveling waves on the operation board, the coupled standing waves can change the infiltration performance of the liquid drops and promote the inside of the liquid drops to form microflows, and the microflows can uniformly mix the components in the liquid drops; the traveling wave drives the droplet to move between the stagnation points, and because the infiltration gradient of the center of the stagnation point is the lowest, the droplet always moves from the center of one stagnation point to the center of the other stagnation point, and the precise control of the position of the droplet is realized. When a plurality of liquid drops are positioned in the stagnation point of the same longitudinal row, the traveling wave is absorbed by the liquid drop closest to the wave propagation direction, and the other liquid drops in the longitudinal row can hardly absorb the traveling wave, so that the liquid drop closest to the wave propagation direction is driven to enter the stagnation points of the other liquid drops in the longitudinal row, and the liquid drops are merged under the combined action of the surface wave and the infiltration gradient. The different vertical rows have different horizontal positions and different infiltration gradients, and liquid can only enter stagnation points of different vertical rows at specific positions. When the liquid drop passes through a specific microstructure, the liquid drop is split under the combined action of an infiltration ladder, a capillary effect and surface acoustic waves.
The invention divides the surface into stationary points by the infiltration gradient, realizes the accurate driving of the liquid drop, and simultaneously, the liquid drop in a certain size span range can be accurately positioned. The stagnation points of different longitudinal rows are only communicated with each other through the infiltration gradient, so that the ordered driving of multiple liquid drops is realized. When the liquid drop passes through a specific microstructure, the liquid drop is split under the combined action of the infiltration gradient, the capillary effect and the surface acoustic wave. Meanwhile, the operation board is adhered to the lithium niobate substrate and can be torn down for replacement, so that the maintenance cost is greatly reduced. The operation panel is a two-dimensional platform, and has advantages such as maneuverability is strong and other equipment of easy integration. The invention has simple and portable structure and strong operability, and can realize non-contact accurate driving of multiple liquid drops and merging and splitting of the liquid drops. The sample loss is less, the reaction time is short, and the method can be applied to various biochemical micro-droplet operations.
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The above-mentioned embodiments, objects, technical solutions and advantages of the present invention are further described in detail, it should be understood that the above-mentioned embodiments are only illustrative of the present invention and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A multi-droplet operation device based on surface acoustic waves is characterized by comprising a lithium niobate substrate, N interdigital electrodes, an operation plate, vibration absorption glue and a control module, wherein N is a natural number more than or equal to 3;
the N interdigital electrodes and the operation plate are arranged on the lithium niobate substrate, wherein the operation plate is arranged in the center of the lithium niobate substrate, and the N interdigital electrodes are uniformly arranged around the circumference of the operation plate;
a planned path for liquid drop driving and liquid drop reaction is arranged on the operating board; the planning path comprises a plurality of stagnation points and a plurality of connecting grooves for communicating the stagnation points, wherein the stagnation points are ridge microstructures forming a wetting gradient and used for adsorbing liquid drops;
the control module is electrically connected with the N interdigital electrodes respectively and is used for controlling the N interdigital electrodes to emit corresponding liquid drops on the surface acoustic wave driving operation panel to move on a planned path, so that the driving, merging and splitting of multiple liquid drops are realized;
the vibration absorption glue is arranged on the lithium niobate substrate to form a closed loop including the lithium niobate substrate and the N interdigital electrodes, and is used for absorbing overflowing surface acoustic waves.
2. A surface acoustic wave based multiple droplet operation device as claimed in claim 1, further comprising N reflecting gratings, wherein said N reflecting gratings are disposed outside said N interdigital electrodes in a one-to-one correspondence, and are all located in a closed loop formed by vibration absorption glue for enhancing the driving force of the surface acoustic wave to the liquid on the operation panel.
3. A surface acoustic wave based multiple droplet operation device as claimed in claim 1, wherein the control module comprises a signal source and a power amplifier, the signal source is used for generating a sinusoidal signal, and the signal is amplified by the power amplifier and then acts on the interdigital transducer to generate surface acoustic waves.
4. A surface acoustic wave-based multi-droplet operation device according to claim 1, wherein the operation panel is attached to the lithium niobate substrate by gluing.
5. A surface acoustic wave based droplet actuator as claimed in claim 1, wherein N is 4 and the interdigital electrode has a thickness in the range of 100-200 nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211343734.0A CN115672227A (en) | 2022-10-31 | 2022-10-31 | Many liquid drops operating means based on surface acoustic wave |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211343734.0A CN115672227A (en) | 2022-10-31 | 2022-10-31 | Many liquid drops operating means based on surface acoustic wave |
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| Publication Number | Publication Date |
|---|---|
| CN115672227A true CN115672227A (en) | 2023-02-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211343734.0A Pending CN115672227A (en) | 2022-10-31 | 2022-10-31 | Many liquid drops operating means based on surface acoustic wave |
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| CN (1) | CN115672227A (en) |
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- 2022-10-31 CN CN202211343734.0A patent/CN115672227A/en active Pending
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