CN115656531B - Integrated pesticide residue standardized rapid detection box and detection method - Google Patents
Integrated pesticide residue standardized rapid detection box and detection method Download PDFInfo
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Abstract
The invention relates to a detection box and a detection method for integrated standardized and rapid detection of pesticide residue, which comprises a detection box body, wherein a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body; the detection box and the detection method are simple in operation, and can finish all detection operations of sample addition, sample extraction, sample transfer, reagent addition, uniform mixing, incubation reaction and detection contrast in the detection box only by a few simple external operations, and detection can be finished without professional operators and professional experimental environments; and the detection process is located in the detection box, the detection environment is stable, the influence of human factors on the detection process is reduced, the stability of the detection process is ensured, a sample detection cabin and a comparison cabin are arranged in the detection box for comparison, whether pesticide residues exist in the sample detection cabin can be detected quickly during optical detection, and the detection efficiency is high.
Description
Technical Field
The invention relates to the technical field of pesticide residue detection, in particular to a detection box and a detection method for integrated standardized and rapid detection of pesticide residue.
Background
The rapid detection of pesticide residues in vegetables and fruits is a very important work in the practical management and control of food safety at present. The traditional method for rapidly detecting pesticide residues mostly adopts a method of standard GB/T5009.199 enzyme inhibition rate and an immunochromatography detection method of various markers (colloidal gold, fluorescent microspheres and quantum dot microspheres) which are popular in recent years. The pretreatment of the two methods is relatively simple, in a laboratory, the peel of fruits and vegetables or She Jiansui is taken to be 1cm square, a certain amount of the fruit and vegetable is weighed and put into an extraction tank, a specific extracting solution is added, after oscillation extraction, a certain amount of liquid to be detected is manually absorbed in a test tube to react with various reagents under different time and temperature conditions, and finally the test result is obtained by measuring and reading the value on equipment. The prior method for quickly detecting pesticide residues still depends too heavily on operators in the pretreatment and detection processes, the strict standardization of process control is difficult to realize, and the stability and reliability of results are not high.
Disclosure of Invention
The invention aims to solve the technical problem that aiming at the defects in the prior art, the invention provides the detection box and the detection method which are simple, convenient, stable, reliable, high in efficiency, independent of laboratories and professional experimenters, standardized and capable of realizing automatic detection, and meets the requirements of the agricultural production and food safety field on rapid and accurate detection of pesticide residues.
The technical scheme adopted by the invention for solving the technical problems is as follows: an integrated pesticide residue standardized rapid detection box comprises a detection box body, wherein a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body; a drainage pipeline is arranged in the sampling cabin; a liquid inlet of the drainage pipeline extends downwards into the liquid in the sampling cabin, and a liquid outlet extends upwards to be communicated with the upper end of the sample detection cabin; the top of the sample detection cabin is provided with a connecting interface for connecting a negative pressure generator; the negative pressure generator drives the liquid in the sampling cabin to flow into the sample detection cabin along the drainage pipeline through the connecting interface; the detection box body is also internally provided with a first reagent adding component for adding a reagent into the sample detection cabin and a second reagent adding component for adding the same reagent into the contrast cabin; the outer side surface of the sample detection cabin and the outer side surface of the comparison cabin are both provided with light-transmitting light detection windows;
the integrated pesticide residue standardized rapid detection box comprises a first reagent adding assembly, a second reagent adding assembly and a detection module, wherein the first reagent adding assembly comprises a plurality of mutually independent first reagent tubes which are arranged in a sample detection cabin; the top of the first reagent tube is provided with a first protective cover, and the bottom of the first reagent tube is provided with a first drainage valve for preventing the reagent in the first reagent tube from flowing out; the first liquid discharge valve can be opened by external force to enable the reagent in the first reagent tube to flow into the sample detection cabin;
the integrated pesticide residue standardized rapid detection box comprises a first reagent adding assembly, a second reagent adding assembly, a control cabin and a detection module, wherein the first reagent adding assembly comprises a plurality of first reagent tubes which are arranged in the control cabin and are independent from each other; the top of the second reagent tube is provided with a second protective cover, and the bottom of the second reagent tube is provided with a second liquid discharge valve for preventing the reagent in the second reagent tube from flowing out; the second liquid discharge valve can be opened by external force to enable the reagent in the second reagent tube to flow into the control cabin;
the integrated pesticide residue standardized rapid detection box is characterized in that the liquid outlet of the drainage pipeline is positioned above the liquid level of the mixed liquid in the sample detection cabin;
the integrated pesticide residue standardized rapid detection box is characterized in that the detection box is also provided with sealing films for sealing the sampling cabin, the connecting interface, the first reagent adding component and the second reagent adding component; the sealing film is tightly attached to the outer edge of the detection box body through hot pressing or gluing;
the integrated pesticide residue standardized rapid detection box provided by the invention is characterized in that two optical detection windows are positioned below the detection box body;
the invention also discloses a detection method for the integrated standardized rapid detection of pesticide residue, wherein the detection box comprises a detection box body, a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body, and buffer solutions without pesticide are arranged in the sampling cabin and the comparison cabin, wherein the buffer solution in the sampling cabin is an extracting solution, and the buffer solution in the comparison cabin is a comparison solution; the detection method comprises the following steps:
the invention relates to a detection method for integrated pesticide residue standardized rapid detection, wherein,
s10: adding a sample to be detected into the sampling cabin, and fully soaking the sample to be detected in the extracting solution in the sampling cabin to obtain a detection sample liquid of the sample to be detected;
s20: driving the detection sample liquid in the S10 to flow into the sample detection cabin along a drainage pipeline by using a power device;
s30: adding a reagent in a first reagent adding assembly and the same reagent in a second reagent adding assembly into the detection sample liquid in the sample detection chamber and the contrast liquid in the contrast chamber respectively to obtain a to-be-detected liquid and a standard liquid;
s40: applying a heat source not exceeding 110 ℃ to the bottom of the detection box, and simultaneously incubating the solution to be detected and the standard solution;
s50: simultaneously carrying out spectrophotometry detection on the liquid to be detected and the standard liquid, comparing the light detection result of the liquid to be detected with the light detection result of the standard liquid, and if the light detection result of the liquid to be detected is different from the light detection result of the standard liquid, determining that the liquid to be detected contains pesticide;
the invention relates to a detection method for integrated pesticide residue standardized rapid detection, wherein the outer side surface of a sample detection cabin and the outer side surface of a comparison cabin are both provided with light-transmitting light detection windows; external light respectively carries out spectrophotometric detection on the liquid to be detected and the standard liquid through the two light detection windows;
the invention relates to a detection method for integrated pesticide residue standardized rapid detection, wherein in S20, a power device is a negative pressure generator connected with a connecting interface at the top of a sample detection cabin; generating negative pressure in a sample detection chamber through the negative pressure generator, wherein the detection sample liquid in the sampling chamber automatically flows into the sample detection chamber;
the invention discloses a detection method for integrated pesticide residue standardized rapid detection, wherein the buffer solution in the sampling cabin and the buffer solution in the control cabin are distilled water.
The invention has the beneficial effects that: the integrated pesticide residue standardized rapid detection box and the detection method have ingenious design, and a sampling cabin, a sample detection cabin, a comparison cabin, a first reagent adding component and a second reagent adding component are arranged in the detection box; buffer solutions are preset in the sampling cabin and the comparison cabin, when in detection, a sample is placed in the sampling cabin to be fully soaked in the sampling cabin, a negative pressure generator is started to enable the sample detection cabin to be in negative pressure, liquid with the sample automatically flows into the sample detection cabin from the sampling cabin along a drainage pipeline, then a first reagent assembly and a second reagent assembly are started to respectively add same reagents into the sample detection cabin and the comparison cabin, and after oscillation and mixing, spectrophotometric detection is respectively carried out on the liquid in the sample detection cabin and the liquid in the comparison cabin through light rays; the detection box can complete all detection operations of sample addition, sample extraction, sample transfer, reagent addition, uniform mixing, incubation reaction and detection contrast in the detection box only by a few simple external operations, and can complete detection without professional operators and professional experimental environments; and the detection process is located in the detection box, the detection environment is stable, the influence of human factors on the detection process is reduced, the stability of the detection process is ensured, a sample detection cabin and a comparison cabin are arranged in the detection box for comparison, whether pesticide residues exist in the sample detection cabin can be detected quickly during optical detection, and the detection efficiency is high.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be further described with reference to the accompanying drawings and embodiments, wherein the drawings in the following description are only part of the embodiments of the present invention, and other drawings can be obtained by those skilled in the art without inventive efforts based on the drawings:
FIG. 1 is a schematic structural diagram of an integrated standardized rapid pesticide residue detection kit according to a preferred embodiment of the present invention;
FIG. 2 is a schematic diagram of the structure of the sample extraction state of the integrated standardized rapid pesticide residue detection kit according to the preferred embodiment of the present invention;
FIG. 3 is a schematic diagram of the sample injection status of the integrated standardized rapid pesticide residue detection cartridge according to the preferred embodiment of the present invention;
FIG. 4 is a schematic diagram of the structure of the reagent added to the integrated standardized rapid pesticide residue detection kit according to the preferred embodiment of the present invention.
Detailed Description
The terms "first," "second," "third," and "fourth," etc. in the description and claims of the invention and in the accompanying drawings are used for distinguishing between different objects and not for describing a particular order. Furthermore, the terms "include" and "have," as well as any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the invention. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein may be combined with other embodiments.
"plurality" means two or more. "and/or" describes the association relationship of the associated objects, meaning that there may be three relationships, e.g., a and/or B, which may mean: a exists alone, A and B exist simultaneously, and B exists alone. The character "/" generally indicates that the former and latter associated objects are in an "or" relationship.
Also, the terms "upper, lower, front, rear, left, right, upper, lower, longitudinal" and the like, which denote orientation, are used with reference to the attitude and position of the device or apparatus in the present disclosure during normal use.
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention.
The first embodiment is as follows:
the integrated pesticide residue standardized rapid detection box disclosed by the preferred embodiment of the invention is shown in figures 1-4 and comprises a detection box body 100, wherein the detection box body 100 is internally provided with a sampling cabin 101, a sample detection cabin 102 and a comparison cabin 103; it is worth to be noted that the sampling chamber is provided with an opening for the sample 10 to be tested to enter, and the sampling chamber 101 and the comparison chamber 103 are both provided with a buffer solution without pesticide, wherein the buffer solution in the sampling chamber 101 is an extracting solution 108, the buffer solution in the comparison solution 109 is a comparison solution 109, and preferably, the buffer solution is distilled water; a drainage pipeline 104 is arranged in the sampling cabin 101; a liquid inlet of the drainage pipeline 104 extends downwards into the liquid in the sampling cabin 101, and a liquid outlet extends upwards to be communicated with the upper end of the sample detection cabin 102; it is worth to say that the liquid inlet end of the drainage pipeline is positioned at the bottom of the sampling cabin; the top of the sample detection chamber 102 is provided with a connection interface 105 for connecting a negative pressure generator (not shown); a negative pressure generator (not shown) drives the liquid in the sampling chamber 101 to flow into the sample detection chamber 102 along the drainage pipeline 104 through the connection interface 105; it is worth to be noted that, the negative pressure generator is the prior art, and the negative pressure generator can be replaced by an air pump and is used for generating negative pressure in the sample detection cabin, so that the liquid in the sampling cabin automatically flows into the sample detection cabin; the detection box body 100 is also internally provided with a first reagent adding component 200 for adding a reagent into the sample detection chamber 102 and a second reagent adding component 300 for adding the same reagent into the control chamber 103; the reagent in the first reagent adding assembly is the same as the reagent in the second reagent adding assembly; the outer side surface of the sample detection cabin and the outer side surface of the comparison cabin 103 are both provided with light-transmitting light detection windows 106; during detection, light rays simultaneously penetrate through the two optical detection windows to simultaneously perform spectrophotometric detection on the solution to be detected 110 and the standard solution 111, the optical detection result of the solution to be detected 110 is compared with the optical detection result of the standard solution 111, if the optical detection result of the solution to be detected 110 is different from the optical detection result of the standard solution 111, the solution to be detected 110 contains pesticide, and the operation is simple.
The integrated pesticide residue standardized rapid detection box is ingenious in design, the detection process is standardized according to the GB/T5009.199 standard, and a sampling cabin, a sample detection cabin, a comparison cabin, a first reagent adding component and a second reagent adding component are arranged in the detection box; buffer solutions are preset in the sampling cabin and the comparison cabin, during detection, a sample to be detected is added into the sampling cabin, the sample to be detected is fully soaked in an extracting solution in the sampling cabin to obtain a detection sample liquid of the sample to be detected, a negative pressure generator is started to enable the sample detection cabin to be in a negative pressure state, the detection sample liquid in the sampling cabin automatically flows into the sample detection cabin from the sampling cabin along a drainage pipeline, then a first reagent assembly and a second reagent assembly are started to respectively add the same reagents into the sample detection cabin and the comparison cabin, after oscillation and mixing, spectrophotometric detection is respectively carried out on the liquid in the sample detection cabin and the liquid in the comparison cabin through light; the detection kit can complete all detection operations of sample addition, sample extraction, sample transfer, reagent addition, uniform mixing, incubation reaction and detection contrast in the detection kit only by a few simple external operations, and can complete detection without professional operators and professional experimental environments; and the detection process is located in the detection box, the detection environment is stable, the influence of human factors on the detection process is reduced, the stability of the detection process is ensured, the light detection result in the sample detection cabin in the detection box is compared with the light detection result in the comparison cabin, whether pesticide residues exist in the sample detection cabin can be detected quickly, and the detection efficiency is high.
Preferably, the first reagent adding assembly 200 comprises a plurality of mutually independent first reagent tubes 201 arranged in the sample detection chamber 102; the top of the first reagent tube 201 is provided with a first protective cover 202, and the bottom is provided with a first drainage valve 203 for preventing the reagent in the first reagent tube 201 from flowing out; the first drain valve 203 can be opened by an external force to allow the reagent in the first reagent tube 201 to flow into the sample detection chamber 102. Preferably, the first reagent assembly is located at the top of the sample detection chamber; when adding reagent toward the sample test under-deck, only need open first protective cover, rethread stirring rod or other pressure poles push away the intraductal first drainage valve of arbitrary first reagent downwards, make first drainage valve lapse, the intraductal reagent of first reagent flows into the sample test under self gravity in the under-deck, easy operation, safe and reliable.
Preferably, the second reagent adding assembly 300 comprises a plurality of second reagent tubes 301 which are independent from each other and are arranged in the control chamber 103; a second protective cover 302 is arranged at the top of the second reagent tube 301, and a second drain valve 303 for preventing the reagent in the second reagent tube 301 from flowing out is arranged at the bottom; the second drain valve 303 can be opened by external force to allow the reagent in the second reagent tube 301 to flow into the control chamber 103; the second reagent adding assembly is positioned at the top of the sample detection cabin; the structure of the second reagent adding assembly can be the same as that of the first reagent adding assembly, the action principle is also the same, and it is worth explaining that when adding the reagent each time, the first reagent adding assembly and the second reagent adding assembly are the same reagent added, and the amount of the reagent added each time is also the same, and variable factors are reduced.
It is worth mentioning that the first drain valve and the second drain valve can be controllably opened under the action of pressure, mechanical thrust or electromagnetic force.
Adding the reagent in the first reagent adding component 200 and the same reagent in the second reagent adding component 300 into the detection sample liquid in the sample detection chamber 102 and the contrast liquid 109 in the contrast chamber 103 respectively to obtain a to-be-detected liquid 110 and a standard liquid 111; simultaneously carrying out spectrophotometric detection on the liquid to be detected 110 and the standard liquid 111, comparing the light detection result of the liquid to be detected 110 with the light detection result of the standard liquid 111, and if the light detection result of the liquid to be detected 110 is different from the light detection result of the standard liquid 111, determining that the liquid to be detected 110 contains pesticide;
preferably, before spectrophotometric detection, a heat source not higher than 110 ℃ can be applied to the bottom of the detection box, and the solution to be detected and the standard solution are incubated to accelerate the reaction.
Preferably, the liquid outlet of the drainage tube 104 is located above the liquid level of the mixed liquid in the sample detection chamber 102, so that the liquid in the extraction chamber can flow into the sample detection chamber in a single direction, and the liquid in the extraction chamber can flow into the sample detection chamber smoothly.
Preferably, the detection box is further provided with a sealing film 107 for sealing the sampling chamber 101, the connection interface 105, the first reagent adding assembly 200 and the second reagent adding assembly 300; the sealing film 107 is tightly attached to the outer edge of the detection box body 100 by hot pressing or gluing; because the top in sample cabin is equipped with the opening, sample test cabin accessible connection interface and external connection, it is sealed with sample cabin, connection interface, first reagent interpolation subassembly and second reagent interpolation subassembly through the seal membrane, can guarantee that the detection box is in encapsulated situation all the time when not using, can effectively prevent in impurity such as external gas, dust gets into the detection box, influences the internal environment, and the testing result is inaccurate when leading to detecting.
Preferably, the sealing film 107 is also provided with an easy-to-tear opening, so that the sealing film can be opened without using an external tool during use, and the operation is convenient.
Preferably, the two light inspection windows 106 are located below the detection box body 100, so that a user can directly observe the light inspection result in the sample detection chamber and the light inspection structure in the comparison chamber.
Example two:
the invention discloses a detection method for detecting organophosphorus and carbamate pesticides in Chinese cabbage, which is applied to a detection box in the first embodiment and comprises the following steps:
the detection box is pre-filled with 5 ml of buffer solution for extraction in the extraction cabin, 2ml of buffer solution for comparison in the comparison cabin, the detection cabin is empty, and three reagent tubes in the first reagent adding assembly and the second reagent adding assembly above the sample detection cabin and the comparison cabin are pre-filled with three reagents for detecting pesticide residues, namely an enzyme reagent, a color developing agent and a substrate.
Sample treatment: sampling Chinese cabbage according to a standard to obtain a sample for detection, transferring the sample to an extraction cabin of a detection box, and soaking the sample to be detected in a buffer solution; and horizontally shaking the detection box for 1 minute to fully soak the sample to be detected in the buffer solution to obtain the detection sample liquid of the sample to be detected.
Sample introduction: and applying negative pressure to the connection interface at the upper part of the sample detection chamber, wherein the negative pressure is formed in the sample detection chamber, and the air pressure in the extraction chamber is greater than that in the sample detection chamber, so that the detection sample liquid in the extraction chamber flows into the sample detection chamber along the drainage pipeline.
Adding a reagent: opening first liquid discharge valves of two reagent pipes of the enzyme reagent and the color developing agent in the first reagent adding assembly, adding the enzyme reagent and the color developing agent into the sample detection cabin to be mixed with the detection sample liquid, simultaneously opening second liquid discharge valves of two reagent pipes of the enzyme reagent and the color developing agent in the second reagent adding assembly, and adding the enzyme reagent and the color developing agent into the control cabin to be mixed with the control liquid; the cartridge was shaken horizontally for 30 seconds while incubating for 10 minutes. After the incubation is finished, a first liquid discharge valve of a substrate reagent tube in the first reagent adding assembly is opened, the substrate reagent is added into the sample detection cabin to obtain the liquid to be detected, a second liquid discharge valve of the substrate reagent tube in the second reagent adding assembly is opened, and the substrate reagent is added into the control cabin to obtain the standard liquid.
Sample detection: and after the horizontal shaking detection box is uniformly mixed for 30 seconds, performing countdown photometric change detection on a photometric window of the sample detection cabin and a photometric window of the comparison cabin for 3 minutes by light.
Control reference: the light detection result of the comparison cabin provides reference comparison for the light detection result of the sample detection cabin, and the enzyme inhibition rate of the sample to be detected can be obtained through calculation so as to determine whether pesticide residues exist in the sample to be detected.
The principle is as follows: under certain conditions, organophosphorus and carbamate pesticides have an inhibiting effect on the normal function of cholinesterase, and the inhibition rate is positively correlated with the concentration of the pesticides. Normally, enzyme catalyzes the hydrolysis of nerve conduction metabolites (acetylcholine), the hydrolysis product reacts with a color developing agent to generate a yellow substance, a spectrophotometer is used for measuring the change value of absorbance along with time at 412nm, the inhibition rate is calculated, and whether organophosphorus or carbamate pesticides exist in a sample can be judged through the inhibition rate.
The process of "measuring the change in absorbance with time at 412nm with a spectrophotometer" in the above example is generally referred to as "photodetection" operation in the process of measuring the pesticide residue.
Example three:
the invention discloses a detection method for detecting sulfur dioxide residue in tremella, which is applied to a detection box in the first embodiment and comprises the following steps:
9.5 ml of extraction buffer solution is preloaded in the extraction cabin of the detection box, 2ml of comparison buffer solution is preloaded in the comparison cabin, the sample detection cabin is empty, and two reagent tubes of a first reagent adding assembly and a second reagent adding assembly above the sample detection cabin and the comparison cabin are respectively preloaded with two reagents for sulfur dioxide rapid detection used for testing: reagent A and reagent B.
Sample treatment: 0.5g of dried tremella sample is weighed according to the standard, and then transferred to an extraction cabin of a sample detection box, so that the sample to be detected is soaked in a buffer solution in the extraction cabin. And horizontally shaking the detection box for 5 minutes to ensure that the sample to be detected is fully soaked in the buffer solution, thereby obtaining the detection sample liquid of the sample to be detected.
Sample introduction: and applying gas negative pressure to a connecting interface at the upper part of the sample detection cabin, wherein the sample detection cabin is in negative pressure, the air pressure in the extraction cabin is greater than that in the sample detection cabin, and the detection sample liquid in the extraction cabin quantitatively flows into the sample detection cabin along the drainage pipeline by 2ml under the action of atmospheric pressure.
Adding a reagent: opening a first liquid discharge valve of a reagent A reagent pipe in the first reagent adding assembly, adding the reagent A into the sample detection cabin to be mixed with the detection sample liquid, and simultaneously opening a second liquid discharge valve of a reagent A reagent pipe in the second reagent adding assembly, adding the reagent A into the contrast cabin to be mixed with the contrast liquid; the cartridge was shaken horizontally for 30 seconds while incubating for 10 minutes. After the incubation is finished, opening a first liquid discharge valve of a reagent B reagent tube in the first reagent adding assembly, adding the reagent B into the sample detection cabin to obtain a liquid to be detected, simultaneously opening a second liquid discharge valve of a reagent B reagent tube in the second reagent adding assembly, and adding the reagent B into the control cabin to obtain a standard liquid; shake and mix for 30 seconds.
Sample detection: and after standing for 10 minutes, carrying out absorbance detection on the light detection window of the sample detection cabin and the light detection window of the comparison cabin through light rays.
Control reference: the light detection result of the comparison cabin provides reference comparison for the light detection result of the sample detection cabin, and the sulfur dioxide concentration of the detected sample can be obtained through calculation so as to determine whether sulfur dioxide residue exists in the sample to be detected.
The principle is as follows: the method comprises the steps of reacting sulfite with sodium tetrachloromercuric oxide to generate a stable complex, reacting with formaldehyde and pararosaniline hydrochloride to generate a mauve complex, measuring absorbance at 550nm by using a spectrophotometer, and quantifying the concentration of sulfur dioxide by adopting a standard curve method.
The hydrochloric pararosaniline ultraviolet spectrophotometric method is a classic sulfur dioxide determination method, and is listed as the 1 st method by the national food standard method of China. However, this method is not suitable for samples containing large amounts of volatile fatty acids or partially colored samples.
The process of "measuring the absorbance at 550nm with a spectrophotometer" in the above example is generally referred to as "photometric" operation of the process of measuring the residual sulfur dioxide.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (5)
1. The integrated pesticide residue standardized rapid detection box comprises a detection box body, and is characterized in that a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body; the sampling cabin is provided with an opening for a sample to be detected to enter, and the sampling cabin and the comparison cabin are both internally provided with buffer solutions without pesticides, wherein the buffer solution in the sampling cabin is an extracting solution, the buffer solution in the comparison solution is a comparison solution, and a drainage pipeline is arranged in the sampling cabin; a liquid inlet of the drainage pipeline extends downwards into liquid in the sampling cabin, a liquid outlet extends upwards to be communicated with the upper end of the sample detection cabin, and a liquid inlet end of the drainage pipeline is positioned at the bottom of the sampling cabin; the top of the sample detection cabin is provided with a connecting interface for connecting a negative pressure generator; the negative pressure generator drives the liquid in the sampling cabin to flow into the sample detection cabin along the drainage pipeline through the connecting interface; the detection box body is also internally provided with a first reagent adding component for adding a reagent into the sample detection cabin and a second reagent adding component for adding the same reagent into the contrast cabin; the outer side surface of the sample detection cabin and the outer side surface of the comparison cabin are both provided with light-transmitting light detection windows;
the first reagent adding assembly comprises a plurality of mutually independent first reagent tubes arranged in the sample detection cabin; the top of the first reagent tube is provided with a first protective cover, and the bottom of the first reagent tube is provided with a first drainage valve for preventing the reagent in the first reagent tube from flowing out; the first liquid discharge valve can be opened by external force to enable the reagent in the first reagent tube to flow into the sample detection cabin;
the second reagent adding assembly comprises a plurality of mutually independent second reagent tubes arranged in the contrast cabin; the top of the second reagent tube is provided with a second protective cover, and the bottom of the second reagent tube is provided with a second liquid discharge valve for preventing the reagent in the second reagent tube from flowing out; the second liquid discharge valve can be opened by external force to enable the reagent in the second reagent tube to flow into the control cabin;
the liquid outlet of the drainage pipeline is positioned above the liquid level of the mixed liquid in the sample detection chamber;
the detection box is also provided with sealing films for sealing the sampling cabin, the connecting interface, the first reagent adding component and the second reagent adding component; the sealing membrane is closely attached to the outer edge of the detection box body through hot pressing or gluing.
2. The cartridge of claim 1, wherein both of the light inspection windows are located below the cartridge body.
3. The detection method is characterized by being applied to the detection box of any one of claims 1-2, and comprising a detection box body, wherein a sampling cabin, a sample detection cabin and a control cabin are arranged in the detection box body, and buffer solutions without pesticides are arranged in the sampling cabin and the control cabin, wherein the buffer solution in the sampling cabin is an extracting solution, and the buffer solution in the control cabin is a control solution; the detection method comprises the following steps:
s10: adding a sample to be detected into the sampling cabin, and fully soaking the sample to be detected in the extracting solution in the sampling cabin to obtain a detection sample liquid of the sample to be detected;
s20: driving the detection sample liquid in the S10 to flow into the sample detection cabin along a drainage pipeline by using a power device;
s30: adding a reagent in a first reagent adding assembly and the same reagent in a second reagent adding assembly into the detection sample liquid in the sample detection chamber and the contrast liquid in the contrast chamber respectively to obtain a to-be-detected liquid and a standard liquid;
s40: applying a heat source not exceeding 110 ℃ to the bottom of the detection box, and simultaneously incubating the solution to be detected and the standard solution;
s50: and simultaneously carrying out spectrophotometry detection on the to-be-detected liquid and the standard liquid, comparing the light detection result of the to-be-detected liquid with the light detection result of the standard liquid, and if the light detection result of the to-be-detected liquid is different from the light detection result of the standard liquid, determining that the to-be-detected liquid contains pesticide.
4. The integrated pesticide residue standardized rapid detection method as claimed in claim 3, wherein the outer side surface of the sample detection chamber and the outer side surface of the control chamber are both provided with light-transmitting light detection windows; and external light respectively performs spectrophotometric detection on the liquid to be detected and the standard liquid through the two light detection windows.
5. The method for detecting the standardized and rapid detection of the pesticide residue as claimed in the claim 4, wherein in the step S20, the power device is a negative pressure generator connected with a connecting interface at the top of the sample detection cabin; and generating negative pressure in the sample detection chamber through the negative pressure generator, wherein the detection sample liquid in the sampling chamber automatically flows into the sample detection chamber.
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| CN111272748A (en) * | 2020-03-09 | 2020-06-12 | 佛山市铬维科技有限公司 | Pesticide residue detection device |
| CN215525546U (en) * | 2021-08-23 | 2022-01-14 | 湖北久华食安生物技术有限公司 | Injection type pesticide residue rapid detection kit |
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| WO2012130107A1 (en) * | 2011-03-25 | 2012-10-04 | 深圳迈瑞生物医疗电子股份有限公司 | Apparatus for automatic analysis and sample analysis method thereof |
| JP5799559B2 (en) * | 2011-04-12 | 2015-10-28 | セイコーエプソン株式会社 | Detection device |
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| CN213986206U (en) * | 2020-12-04 | 2021-08-17 | 广州市来利洪饼业有限公司 | Wheat flour pesticide residue rapid detection equipment |
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| CN111272748A (en) * | 2020-03-09 | 2020-06-12 | 佛山市铬维科技有限公司 | Pesticide residue detection device |
| CN215525546U (en) * | 2021-08-23 | 2022-01-14 | 湖北久华食安生物技术有限公司 | Injection type pesticide residue rapid detection kit |
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