CN115645314A - Composition for regulating and controlling skin microecological diversity - Google Patents

Abstract

The invention provides a composition for regulating and controlling skin microecological diversity, which comprises hydroxyethyl urea, tremella fruiting bodies, panthenol and hydrolyzed sericin. The invention also relates to the use of a composition comprising hydroxyethyl urea, tremella entities, panthenol and hydrolysed sericin for the preparation of a skin external preparation for regulating the microecological diversity of the skin.

Description

Composition for regulating and controlling skin microecological diversity

Technical Field

The invention relates to the fields of natural medicinal chemistry and cosmetics, in particular to a composition of tremella fruiting body, hydroxyethyl urea and D-panthenol combined hydrolyzed sericin, which can regulate and control the microecological diversity of skin.

Background

Human body microorganisms play a key role in human body physiology and organ functions, and control the occurrence and development of diseases, such as diabetes, obesity and other diseases related to the imbalance of intestinal microorganisms, asthma related to the imbalance of pharyngeal microorganisms, and the like. The skin, which is an important boundary organ of anatomy and physiology, actively or passively contacts various external substances, is also a main barrier for the body to contact with the outside, and inhabits a variety of microorganisms, the number of which is measured in trillions, and the interaction of skin microorganisms, hosts and the external environment constitutes the skin microecological balance. The human facial skin is rich and diverse in microbial flora, including resident bacteria regularly colonizing specific sites or ages, such as staphylococcus epidermidis, propionibacteria and the like, the most dominant bacteria, and transient colonizations, called passerby, such as staphylococcus aureus, corynebacterium, streptococcus and the like. Most of microorganisms are harmless to organisms and even beneficial to the organisms, and are planted according to different ecological environments on the surface of the skin, so that delicate balance is maintained between the microorganisms and tissue cells on the surface of the skin to form a 'micro-ecological barrier'. The normal microbial flora plays a positive role in maintaining the physiological functions of the organism, and the disturbance of the micro-ecological environment caused by any physical or chemical factors can affect the health of the organism, such as: cosmetics, drugs, clothing, etc. The existence of these foreign substances has a great possibility of disrupting the balance of the skin surface microecology, causing various skin problems, and studies have shown that skin problems such as atopic dermatitis, acne, dandruff, sensitive redness, and the like are closely related to the imbalance of skin microorganisms.

With the increasing use frequency of cosmetics, complex components of the cosmetics comprise a plurality of chemical substances, such as preservatives, essences, fragrances, antioxidants, deodorants and the like which are added for ensuring that the cosmetics have good antiseptic performance in the shelf life and the use period, and a large number of Chinese and foreign researches show that the preservatives are one of the main derivatives of skin sensitization. A follow-up survey by european scholars for up to 11 years showed that preservatives account for up to 58% of allergies. In addition, long-term use of antiseptic can disturb skin resident flora, thereby changing skin micro-ecological environment, such as changing pH, humidity, oil content, etc., affecting secretion function of sweat gland and sebaceous gland, and preventing skin self-cleaning and metabolism function. In the practical application of cosmetic formulations, the usage amount of preservatives in different forms of cosmetics varies greatly, and therefore, the effect of the type of preservative on the flora is only considered and cannot truly reflect the problems encountered in the practical application. For example, the dosage of the mask liquid of the facial patch type cosmetic is about 25mL per time, which is 20-50 times of the single dosage (about 0.75-1.25 g) of the common cream type cosmetic, and the single dosage of the preservative added in an equal ratio can be simultaneously increased by 20-50 times, so that the disturbance degree of the microecological flora is obviously different. The face mask products are different from cosmetics recently, and become popular for consumers. The frequency of use by some consumers may even reach twice a day. If the degree of disturbance of the mask products on the microecological flora is larger, the mask products have larger hidden danger for the microecological health of consumers.

Therefore, the invention provides a composition with excellent conditioning effect on the microecological balance on the skin, and the composition can also be used as an effect additive for facial mask products and used for regulating and controlling the microecological diversity.

Disclosure of Invention

In one aspect, the invention provides a composition for regulating and controlling skin microecological diversity, comprising hydroxyethyl urea, tremella fruiting body, panthenol and hydrolyzed sericin.

In a preferred embodiment, the ratio of hydroxyethyl urea: tremella sporophore: panthenol: the weight ratio of the hydrolyzed sericin is 0.1-10:0.1-3:0.1-4:0.1-1. In a more preferred embodiment, the ratio of hydroxyethyl urea: tremella sporophore: panthenol: the weight ratio of the hydrolyzed sericin is 6:1:0.1-4:0.1-1.

In a preferred embodiment, the composition of the present invention is prepared in the form of a mask.

In a preferred embodiment, the composition of the invention is in lyophilized form. In a more preferred embodiment, the lyophilized form is formulated into a tablet, pill, or floe form.

In a preferred embodiment, the composition of the invention is free of preservatives.

In another aspect, the present invention relates to the use of a composition comprising hydroxyethyl urea, tremella entity, panthenol and hydrolyzed sericin in the preparation of a skin external preparation for regulating the microecological diversity of skin.

In a preferred embodiment, the external preparation for skin is selected from: lotion, cream and toner. In a preferred embodiment, the external preparation for skin comprises 0.001 to 50% by weight of the composition.

Brief description of the drawings

Fig. 1 shows a picture of a mask mold recess of an embodiment.

Fig. 2 shows the results of cytotoxicity evaluation of test example 3, in which the left side is a cell survival state picture of example 2, and the right side is a cell survival state picture of example 19. Electron microscopy, OLYMPUS BX53, magnification 40X was used.

Figure 3 (a) shows the change in diversity Shannon index (OTU level) between groups before and after mask use. The Shannon index is used to assess the abundance and uniformity of species composition in a sample. A larger index indicates a richer variety of species and a higher biodiversity for the ecology. Conversely, the lower the Shannon index, the lower the diversity of the skin microecological flora.

Figure 3 (b) shows the diversity Chao1 index comparison (OTU level) between groups before and after mask use. The Chao1 index estimates the index of the number of OTUs contained in a sample, with larger numbers representing more species contained in the sample.

FIG. 4 shows the abundance of species composition at the genus level, where the bacteria composition at the genus level is dominated by Propionibacterium and Staphylococcus, and the five groups are more than 1% abundant and there are 18 taxonomically named dominant bacteria, accounting for 85% of the total amount of skin bacteria. Pseudomonas (Pseudomonas) (0.47 +/-0.52vs 2.18 +/-1.90, P = 0.019) and Xanthomonas (Xanthomonas) (0.06 +/-0.13vs 2.11 +/-2.07, P = 0.006) were significantly increased after the B mask was used. Staphylococcus (27.31 + -18.74vs 17.01 + -11.18, p = 0.028) is significantly reduced after the E facial mask is used, while the B facial mask has no significant change (21.08 + -16.4 vs 19.44 + -15.41, p = 0.829), which indicates that the preservative in the E facial mask inhibits the growth of staphylococcus epidermidis.

Detailed Description

The invention provides a freeze-dried composition for regulating and controlling the microbial diversity of facial skin, which can be loaded on a daily mask cloth and used as a substitute of a daily mask product. Can also be made into tablet, pill, flocculent product by freeze-drying. Can also be used as functional additive to be added into cosmetic emulsion, cream, toner, etc. for regulating and controlling skin microecological diversity.

The long-term use of the freeze-dried composition has a positive effect on facial skin microorganisms. The Miseq sequencing platform in the high-throughput sequencing technology is selected for determination, and the use of the freeze-dried composition mask has positive and positive influence on the composition of skin microflora, so that the advantage of the freeze-dried composition mask on the health of human skin is very obvious.

The invention provides an effective composition with better performance. Meanwhile, the composition with high safety and high bioavailability is formed by adopting the modern cosmetic technology for pretreatment.

To provide a more concise description, some of the quantitative representations presented herein are not modified by the term "about". It is understood that each quantity given herein is intended to refer to the actual given value, regardless of whether the term "about" is explicitly used, and also to refer to the approximation to such given value that would reasonably be inferred by one of ordinary skill in the art, including approximations due to experimental and/or measurement conditions for such given value.

To provide a more concise description, some quantitative expressions are recited herein as a range from about an X amount to about a Y amount. It should be understood that when a range is recited, the range is not limited to the upper and lower limits recited, but includes the entire range from about the X amount to about the Y amount or any amount therebetween.

Hydroxyethyl urea

Hydroxyethyl urea, a derivative of urea, of formula C ₃ H ₈ N ₂ O ₂ And the molecular weight is 104.1. The hydroxyethyl urea is colorless to light yellow transparent liquid, can well permeate into the stratum corneum of the skin, enhances the moisture content of the skin and relieves dryness. In some embodiments, the composition for modulating skin micro-ecological diversity comprises hydroxyethyl urea.

In some embodiments, the present compositions comprise 1-10 wt.% hydroxyethyl urea. In a preferred embodiment, the present compositions comprise 1-5 wt.% hydroxyethyl urea.

Tremella fruiting body

The tremella fruiting body is a synthetic body of amino acids and nucleic acids formed by main chain alpha- (1-3) mannose, side chain fucose, xylose and glucuronic acid, and is acidic heteropolysaccharide with polymerization degree of about 100 ten thousand. The tremella sporophore forms a colorless, transparent, viscous liquid when dissolved in water. Has good skin moisturizing performance. In some embodiments, the composition for modulating skin microecological diversity comprises a white fungus fruit body.

In some embodiments, the present compositions comprise 0.1-5 wt.% of the fruiting body of tremella fuciformis. In a preferred embodiment, the present composition comprises 0.1-1 wt.% of tremella fruiting bodies.

Panthenol

Panthenol, also known as provitamin B5, is a precursor of vitamin B5 and has a molecular formula C ₉ H ₁₉ NO ₄ Molecular weight 205.25. In some embodiments, D panthenol is used herein. The D panthenol is absorbed by skin and is quickly converted into pantothenic acid through oxidation, and is converted into acetyl coenzyme A in epidermal cells, so that the D panthenol participates in the skin metabolism process, and has the functions of moisturizing and repairing. In some embodiments, the composition that modulates skin micro-ecological diversity comprises panthenol.

In some embodiments, the compositions herein comprise 1-8% by weight panthenol. In a preferred embodiment, the present composition comprises 1-5 wt.% panthenol.

Hydrolyzed sericin

The stratum corneum of the skin should retain 10-20% of water to make the skin plump and elastic. When the water content in the stratum corneum layer of the skin is below 10%. The skin is dry and even cracked. The stratum corneum can therefore contain a certain amount of water, on the one hand due to the fact that the sebum membrane prevents water evaporation, and on the other hand contains natural moisturizing factors (nmf), which play an extremely important role in skin moisturization. The natural moisturizing factor mainly comprises amino acids, pyrrolidone carboxylic acid, lactate, urea, saccharides, organic acid, polypeptide and other substances. If these water-soluble components are removed from the stratum corneum, the moisture absorption of the skin will be lost.

The hydrolyzed sericin is derived from silk, is a membrane layer natural macromolecular protein for protecting the outer part of the silk, accounts for about 20 to 30 percent of the weight of the silk, and is rich in polar amino acids such as serine, aspartic acid, glutamic acid and the like. The hydrolyzed sericin has similar structure with amino acid in Natural Moisturizing Factor (NMF) of human skin, has nitrogen content of about 15%, and has good effects of moisturizing, resisting oxidation, and nourishing skin.

Sericin contains 18 amino acids, more than 90% of the amino acids can be absorbed by human body, more than 17% of the amino acids necessary for human body, 80.41% of polar amino acids and 19.59% of non-polar amino acids, has good moisture absorption and release performance, and can play a role similar to natural moisture retention factors when added into cosmetics. In addition, sericin is a globular protein, the secondary structure of which is mainly random coil and accounts for 41.6%, and beta-sheet and beta-turn account for 30.5% and 23.6% of the total amount respectively, the content of alpha-helix is low and only accounts for 4.3%, and the sericin has an antioxidant function, can resist the corrosion of sunlight, microwaves, chemical substances and atmospheric pollutants to skin, can well inhibit the generation of active oxygen, prevents skin wrinkling and aging, and has a better effect than vitamin C with an antioxidant function; it is excellent in safety because of its natural properties and bioaffinity. However, in the contact between amino acids and cells, the concentration, acidity or basicity, isoelectric point, and other factors of some amino acids may have a certain toxicity to cells.

In some embodiments, the compositions of the present application comprise 0.1 to 1% by weight of hydrolyzed sericin. In a preferred embodiment, the present composition comprises 0.1 to 0.5 wt.% of hydrolyzed sericin.

Composition for regulating and controlling skin microecological diversity

The facial skin micro-ecology maintains good diversity distribution, namely the balance of micro-ecological microbial communities is the basis of skin health. The skin problems begin with a disruption of the balance and diversity of the skin's micro-ecological microflora, i.e. one or several microorganisms become dominant, such as the occurrence of facial acne, due to an imbalance of propionibacterium acnes on the faceIs highly correlated with excessive reproduction; the development of atopic skin inflammation, superficially rough, dry, barrier-breaking, is actually associated with the excessive proliferation of staphylococcus aureus. The overgrowth of Candida albicans in the closed environment of diaper rash causes the number and diversity of other healthy flora to be inhibited on the skin surface. In addition, for example, the nocturnal odor is associated with diphtheria-like bacillus, the dandruff is associated with pityrosporum orbiculare, and the like. Human skin surface colonizing human body with a large number of microorganisms (10) ⁶ Per cm ² ) And these microorganisms and the different niches of the skin surface form a complex ecosystem-the skin micro-ecology. The flora on the skin surface is divided into resident flora and temporary flora, and most of the flora is beneficial to our bodies, such as cleaning garbage (apoptotic cells) on the skin surface, decomposing redundant lipid, resisting invasion of external pathogenic bacteria through competitive inhibition, and the like. When the balance and diversity relationship is broken, the microecosystem, which is the first barrier of human skin, is likely to be attacked by opportunistic pathogens, causing a variety of infectious diseases and skin problems, and the skin functions are disturbed.

The invention unexpectedly finds that the composition containing hydroxyethyl urea, tremella sporocarp, panthenol and hydrolyzed sericin can greatly enrich the diversity and abundance of skin flora and promote skin health. The invention unexpectedly discovers that the combination of panthenol and sericin has very obvious synergistic effect of promoting the diversity of skin micro-ecological flora. Although the individual use of panthenol or sericin does not adversely affect the diversity and abundance of skin micro-ecological colonies and basically maintains the existing balance of the micro-ecological system, the individual use of panthenol or sericin does not positively enrich and diversify the skin micro-ecological colonies. Only after the panthenol and the sericin are used in combination, the function of regulating and controlling the skin micro-ecological balance can be realized. This undoubtedly opens up a new solution for the health of the skin and the resolution of skin problems.

The present invention also finds that the preservative has a significant devastating effect on the health and balance of the skin surface micro-ecology. From the action mechanism of the preservative, the microbial cell wall is destroyed or the formation of the microbial cell wall is inhibited; secondly, the cell membrane of the microorganism is destroyed or the function of the cell membrane is influenced, so that the substance in the microorganism cell leaks to cause death; and thirdly, inhibiting enzyme or protein synthesis or denaturing protein in the microbial cell, resulting in death of the microbe. Thus, preservatives do not pose irritation, safety and toxicological problems to the human skin itself at a range of concentrations from the human safety end, but have potential impact and possible long-term destructive effects from the point of view of microbial flora diversity and abundance.

In some embodiments, the hydroxyethyl urea: tremella sporophore: panthenol: the weight ratio of the hydrolyzed sericin is 0.1-10:0.1-3:0.1-4:0.1-1. In a preferred embodiment, the hydroxyethyl urea: tremella sporophore: panthenol: the weight ratio of the hydrolyzed sericin is 6:1:0.1-4:0.1-1. In a preferred embodiment, the hydroxyethyl urea: tremella sporophore: panthenol: the weight ratio of the hydrolyzed sericin is 6:1:0.1-4:0.1-0.5.

In a preferred embodiment, the composition for regulating the skin micro-ecological diversity according to the present invention is prepared as a lyophilized product.

Application of composition for regulating and controlling skin microecological diversity

In a preferred embodiment, the composition for regulating the micro-ecological diversity of the skin according to the present invention may be prepared in the form of a mask.

In some embodiments, the composition for regulating the skin micro-ecological diversity can be loaded on a mask cloth and used as a mask after freeze-drying. In a specific embodiment, the freeze-drying conditions are as follows: freezing in a refrigerator at minus 80 ℃ for 2 hours, and freeze-drying in a freeze dryer, wherein the temperature of sublimation drying is minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the temperature of desorption drying is minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 and 6 percent, thus obtaining the loose, cotton-shaped and light facial mask. The facial mask obtained by the freeze vacuum drying preparation process has excellent conditioning application on the microecological balance of skin.

In some embodiments, the compositions for regulating skin micro-ecological diversity of the present invention can be prepared in the form of tablets, pills, and floes.

In other embodiments, the composition for regulating skin micro-ecological diversity of the present invention may be applied to a skin external preparation as a efficacy additive. In some embodiments, the external skin agent is selected from: lotions, creams, lotions, etc. Different amounts are added according to different types of preparations.

The external preparation for skin is a general concept of all ingredients generally used for the external part of the skin. The skin external preparation may be a basic cosmetic, a face makeup cosmetic, a body makeup cosmetic, a hair care cosmetic, etc., and its formulation is not particularly limited and may be appropriately selected according to various purposes. The skin external preparation further contains various cosmetically acceptable vehicles or base excipients depending on the formulation and purpose.

The skin external preparation is preferably a cosmetic, such as a lotion, essence, cream, etc. The composition of the present invention can be used alone or as an additive in an external preparation for skin, and the weight percentage of the composition is 0.001 to 50% (w/w). The preferred weight percentage is 1 to 20 percent (w/w). More preferably 2% to 10% (w/w). The most preferred weight percentage is 3% to 8% (w/w).

Examples

The invention will be further illustrated by the following specific examples. It should be noted that the examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, as many insubstantial modifications and variations of the invention may be made by those skilled in the art in light of the above teachings. Test methods without specifying specific conditions in the following examples are generally performed under conventional conditions or conditions recommended by the manufacturers. All percentages and parts are by weight unless otherwise indicated.

The experimental materials used in the following examples are as follows:

d panthenol, available from Dismann (China) Co.

Hydroxyethyl urea, available from akk Sunuo bell (china) ltd.

Tremella encarpium, provided by Shanghai Huiwen Biotechnology Ltd.

Hydrolyzed sericin, supplied by imperial (china) ltd.

Example 1: preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:0.1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of 80 ℃ hot water, increasing the stirring speed to 400rpm, and continuing for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 2: preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:0.5, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of 80 ℃ hot water, increasing the stirring speed to 400rpm, and continuing for 10 minutes to obtain 500g of the uniform composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 3: preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 4 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:1.5, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 5 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:2, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of 80 ℃ hot water, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (4) standby.

Example 6 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:5, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 7 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:0.1:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 8 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:1:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing the mixture in a refrigerator at the temperature of minus 80 ℃ for 2 hours, and then putting the mixture into a freeze dryer (a vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to be minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the resolution drying is set to be minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled to be 0 to 6 percent, so that the loose, spongy light and thin facial mask is obtained. And (5) standby.

Example 9 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:2.5:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (4) standby.

EXAMPLE 10 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:5:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial-shaped film forming grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each film forming groove, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 11 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:6:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of 80 ℃ hot water, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing the mixture in a refrigerator at the temperature of minus 80 ℃ for 2 hours, and then putting the mixture into a freeze dryer (a vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to be minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the resolution drying is set to be minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled to be 0 to 6 percent, so that the loose, spongy light and thin facial mask is obtained. And (5) standby.

Example 12 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:8:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 13 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:0.1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 14 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:0.5:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of 80 ℃ hot water, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 15 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1.5:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (4) standby.

Example 16 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:2:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing the mixture in a refrigerator at the temperature of minus 80 ℃ for 2 hours, and then putting the mixture into a freeze dryer (a vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to be minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the resolution drying is set to be minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled to be 0 to 6 percent, so that the loose, spongy light and thin facial mask is obtained. And (5) standby.

Example 17 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:2.5:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

EXAMPLE 18 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:3:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (4) standby.

Example 19 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =0.1:1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

EXAMPLE 20 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =1:1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (4) standby.

EXAMPLE 21 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =3:1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

EXAMPLE 22 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 23 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =8:1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (4) standby.

EXAMPLE 24 preparation of the composition

Weighing hydroxyethyl urea according to the weight ratio: tremella sporophore: panthenol: hydrolyzed sericin =10:1:4:1, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm for 10 minutes to obtain 500g of the uniform composition. Facial mask grooves (shown in figure 1) are adopted, and 20 g of composition solution is poured into each mask, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (4) standby.

Example 25

40 g of hydroxyethyl urea was weighed, 460 g of hot water at 80 ℃ was added, and the stirring speed was increased to 400rpm for 10 minutes to obtain 500g of a homogeneous composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 26

Weighing 40 g of tremella fruiting body, adding 460 g of hot water with the temperature of 80 ℃, increasing the stirring speed to 400rpm, and keeping for 10 minutes to obtain 500g of uniform composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 27

40 g of panthenol were weighed out and 460 g of 80 ℃ hot water were added and the stirring speed was increased to 400rpm for 10 minutes to obtain 500g of a homogeneous composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 28

40 g of hydrolyzed sericin was weighed, 460 g of 80 ℃ hot water was added, and the stirring speed was increased to 400rpm for 10 minutes to obtain 500g of a uniform composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming groove, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 29

Weighing hydroxyethyl urea by weight: tremella sporophore: panthenol: hydrolyzed sericin =6:1:4:0, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of hot water at 80 ℃, increasing the stirring speed to 400rpm, and continuing for 10 minutes to obtain 500g of the uniform composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Example 30

Weighing hydroxyethyl urea by weight: tremella sporophore: panthenol: hydrolyzed sericin =6:1:0:0.5, stirring at 250rpm for 5 minutes after mixing to obtain 40 g of the composition, adding 460 g of 80 ℃ hot water, increasing the stirring speed to 400rpm, and continuing for 10 minutes to obtain 500g of the uniform composition. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming groove, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Test example 1: resolubility test

The redissolution test was performed according to the method described in Taobao T/CAFFCI 29-2019, weighing 50mg (. + -. 1 mg) of the test sample into a 10ml stoppered test tube, adding exactly 2ml of distilled water with a pipette, immediately covering the stoppered test tube, holding the test tube with the whole hand, pressing the stoppered test tube with the thumb, shaking the stoppered test tube up and down by about 30cm for 10s, and then standing and observing the test tube. Table 1 shows the redissolution test results.

TABLE 1

The re-solubility of the freeze-dried related product is an important evaluation index of the use experience, and the requirements of people on the use of the product are different in the use process of the skin beautifying preparation. The freeze-dried powder is easy to dissolve, can be quickly and completely dissolved in a short time, is an evaluation index for redissolution of freeze-dried products, and cannot be accepted due to the phenomena of half dissolution, indissolvability and the like.

For examples 1-6, the concentration of hydrolyzed sericin was gradually increased, and the sericin generally had a molecular weight of 14 to 314kD, and it was hydrolyzed to exert its effect to the maximum extent. After the treatment of the hydrolysis process, the adopted hydrolyzed sericin has the relative molecular weight of 5-14 kD, is rich in various amino acid combinations (polypeptides, small molecular proteins and the like), has more high-molecular adhesion and entanglement when the concentration is higher, enhances the binding capacity to water in the freeze-drying process, and is not easy to form a honeycomb freeze-drying structure.

For examples 7 to 12, it can be understood that the structure of panthenol itself is an optically active stereoisomer, which is also called D-N- (α - γ -dihydroxy- β, β -dimethylbutyryl) - β -aminopropanol, has a high viscosity, and although it is soluble in water, the solubility decreases at higher dosages due to its high viscosity and the complex with the solution of the tremella entity, and the lyophilization re-solubility is poor. The high viscosity of the tremella sporocarp dissolved in water reduces the solubility of the hydroxyethyl urea substance mixed with the tremella sporocarp, and the redissolution and the solubility of the tremella sporocarp are greatly influenced.

Examples 31 to 60

The enthusiasm of personalized skin care is not strong, and the aims of quickly beautifying and protecting the skin can be achieved by means of on-site preparation, disposable use and the like for possible customized scenes. Based on this, the compositions prepared in the weight ratio in examples 1 to 30 can also be prepared into skin preparations in a field preparation manner, so that the skin preparations can be directly used for skin care, namely, the long-term storage and stability requirements are not needed, namely, the product is not needed to be used and stored in a freeze-dried form. Thus, the product in the use scene is more concerned about the safety, the efficacy and the like of the product.

The hydroxyethyl urea is weighed according to the weight proportion of the embodiment 1-30 respectively: tremella sporophore: panthenol: hydrolyzing four substances of sericin, mixing, stirring at 250rpm for 5 min to obtain 40 g of composition, adding 460 g of 80 ℃ hot water, increasing the stirring speed to 400rpm, and keeping for 10min to obtain 500g of uniform composition.

Test example 2: evaluation of safety irritation of product by chick embryo chorioallantoic membrane test

Bai Laihang fertilized chicken egg is purchased from Shanghai Guixing poultry Co., ltd, and is put into a Rcom MARU max chick embryo incubator for incubation, the air chamber end is upward, the temperature is 37.6 +/-0.1 ℃, the relative humidity is 46 +/-1%, the eggs are automatically turned once when the eggs are inclined at 45 degrees in the horizontal direction every hour, the air exchange is automatically carried out, and the method is applied to the 10 th day. Taking 10-day-old chick embryo, removing eggshell at the top end of the air chamber, adding 1mL of physiological saline with pre-temperature of 37 ℃ onto the eggshell membrane to moisten the eggshell membrane, and carefully peeling off the eggshell membrane by using small elbow forceps to expose the allantoic membrane (CAM). Placing the chick embryo under a Leica stereomicroscope, magnifying by 16 times, and observing the chick embryo with the observation area of 11.0 multiplied by 8.25mm ² Focus was adjusted to clearly visualize blood flow in the allantoic vessels.

An end-point evaluation method is adopted: the samples prepared for the examples were taken and, after reconstitution with 20ml of deionized water, 0.3ml was added to the CAM. For examples 31-60, 0.3ml of sample was taken directly onto the CAM. Starting timing, after 3min, lightly washing the sample with physiological saline pre-warmed at 37 ℃, observing the degree of CAM blood vessel bleeding, blood vessel thawing and blood coagulation after 30s, respectively rating 0.5,1.0,1.5,2.0,2.5,3.0 according to the light, medium and heavy degrees, performing experiments on each sample by using 6 chick embryos, and finally calculating the stimulation integral sum of the 6 chick embryos. And (3) carrying out irritation grading on the test sample: the total score is less than 1, and no irritation exists; the total score is less than 5 points, and the product is slightly irritant; the total score is less than 10 points, and the irritation is moderate; total score > 10 points, severe irritation. Table 2 shows the results of the test for evaluating the safety stimulation of the product in the chick embryo chorioallantoic membrane test.

TABLE 2

The chick embryo chorioallantoic membrane method is one of evaluation techniques for detecting whether a skin preparation has irritation, which is simple, convenient, economical, short in experimental period and free of special equipment. The preparation is evaluated by observing the stimulation reflection of blood vessels after the allantoic membrane is exposed to the skin preparation (observing the changes of the shapes, colors and permeability of the allantoic membrane blood vessels and a tissue network, and the protein denaturation phenomenon and damage degree), and calculating the stimulation score, so that the sensitivity is very high. As a method for screening a composition for safety and irritability, it is possible to help screen a composition having high cost performance, good safety and irritability. From the results of the four categories of grading, i.e. no irritation, mild irritation, moderate irritation and severe irritation, part of the composition showed very good safety results, especially examples 1,2, 13, 19, 20, 29 and 30. Comparing the irritation results after reconstitution of the same ratio composition and lyophilized formulation, there is a high degree of approximation, as examples 1 and 31, examples 2 and 32, examples 13 and 43, examples 19, 20 and examples 49, 50, etc., all exhibit similar non-irritating safety results. Indicating that these compositions exhibit a high degree of homogeneity during fresh formulation and freeze-drying storage. Therefore, it was further verified in the course of the subsequent cytotoxicity study.

Test example 3: cell Counting Kit-8 (CCK-8) was used to evaluate cytotoxicity of products

The CCK-8 method is a highly sensitive, radioactivity-free colorimetric assay for determining the number of viable cells in a cytotoxicity assay. The reagent contains WST-8 (chemical name: 2- (2-methoxy-4-nitrophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonic acid benzene) -2H-tetrazole monosodium salt), and under the action of an electron carrier (1-methoxy-5-methylphenazinium dimethyl sulfate), dehydrogenase in cell mitochondria can be reduced into orange yellow formazan products with high water solubility, and the amount of generated formazan products is in direct proportion to the amount of living cells. The CCK-8 method can calculate the number of living cells through colorimetric determination, the optical density of the living cells is determined by an enzyme linked immunosorbent assay detector, and the ratio of a test group can be obtained by comparing the optical density with a control group, so that the cytotoxicity of a tested sample is determined.

Human skin keratinocytes (EpiKutis, batch ES 211204) were cultured, 100. Mu.L of cells were added per well in 96-well plates (seeded at a seeding density of 8X 103 cells/well), cells were cultured in a 5% CO2 cell culture chamber (Thermo, 150 i) at 37 ℃ and 3 replicate wells were set. When the cell plating rate in a 96-well plate reaches 40-60%, administration is carried out, namely 10 mu L of culture solution (the mass ratio is 20%) of samples with the concentration prepared by the embodiment is added into each well; adding 10 mu L of culture solution into each well of the control group; after completion of the administration, the 96-well plate was incubated in an incubator (37 ℃ C., 5% CO2) for 24 hours. Add 10. Mu.L of CCK-8 solution to each well, incubate 5% CO2 at 37 ℃ for 1-4 h. The absorbance (OD value) at 450nm was measured using a microplate reader (BioTek, epoch).

Cell viability results calculation the OD values of each test well were divided by the background OD value (no dosing blank), and the OD values of each replicate well were averaged ± SD.

Cell viability% = (dosed cell OD/control cell OD) × 100%.

The cytotoxicity comparison of all samples was performed at a concentration of 20% as tested by experimental data.

Table 3 shows the results of evaluating cytotoxicity of the products by the CCK-8 method.

TABLE 3

Note: "+" indicates that the effect of the sample on mitochondrial activity at the corresponding concentration was different compared to the negative control, "+" is < 0.05.

The stratum corneum of the skin should retain 10-20% of water to make the skin plump and elastic. When the water content in the stratum corneum of the skin is below 10%. The skin is dry and even cracked. The stratum corneum can therefore contain a certain amount of water, on the one hand due to the fact that the sebum membrane prevents water evaporation, and on the other hand contains natural moisturizing factors (nmf), which play an extremely important role in skin moisturization. The natural moisturizing factor mainly comprises amino acids, pyrrolidone carboxylic acid, lactate, urea, saccharides, organic acid, polypeptide and other substances. If these water-soluble components are removed from the stratum corneum, the hygroscopicity of the skin is lost. Sericin contains 18 amino acids, more than 90% of the amino acids can be absorbed by human body, more than 17% of the amino acids necessary for human body, 80.41% of polar amino acids and 19.59% of non-polar amino acids, has good moisture absorption and release performance, and can play a role similar to natural moisture retention factors when added into cosmetics. In addition, sericin is a globular protein, the secondary structure of which is mainly random coil and accounts for 41.6%, and beta-sheet and beta-turn account for 30.5% and 23.6% of the total amount respectively, the content of alpha-helix is low and only accounts for 4.3%, and the sericin has an antioxidant function, can resist the corrosion of sunlight, microwaves, chemical substances and atmospheric pollutants to skin, can well inhibit the generation of active oxygen, prevents skin wrinkling and aging, and has a better effect than vitamin C with an antioxidant function; it is excellent in safety because of its natural properties and bioaffinity. However, in the contact between amino acids and cells, the concentration, acidity or basicity, isoelectric point, and other factors of some amino acids may have a certain toxicity to cells. From the cytotoxicity results, the preferred compositions exhibited substantially lower cytotoxicity through the chick chorioallantoic membrane, but for the specific compositions, examples 1,2, 29, 30 all exhibited very low cytotoxicity, while examples 13, 19, 20 exhibited slight cytotoxicity. The very close cytotoxicity results are shown compared to freshly prepared compositions of the same proportions, further demonstrating that the lyophilized compositions exhibit very good stability and retention of activity of the active over the shelf life. Therefore, in the following micro-ecological test, only the freeze-dried and shaped mask product is used as a test object, and as a further comparative example, a preservative system which is more commonly adopted in the conventional mask is simulated to be used as a wet mask combination.

Example 61: preparation of composition (comparative example)

The preparation method of example 2 was followed to obtain 500g of the homogeneous composition, which was mixed with 1g of phenoxyethanol (0.2% by mass) and 1g of menthone (0.2% by mass) and stirred to mix well. Face-shaped film forming grooves are adopted, and 20 g of composition solution is poured into each film forming, so that the mask solution is uniformly and flatly distributed in the mold. Freezing for 2 hours in a refrigerator at minus 80 ℃, and then putting the facial mask into a freeze dryer (vacuum freeze dryer, model FD-2, beijing Bo Yi kang laboratory instruments Co., ltd.) for freeze drying, wherein the sublimation drying is set to minus 80 to minus 40 ℃, the vacuum degree is 1 to 30Pa, the time is 3 to 21 hours, the analysis drying is set to minus 10 to plus 20 ℃, the vacuum degree is 1 to 30Pa, the time is 1 to 10 hours, and the water content is controlled between 0 to 6 percent, thus obtaining the loose, cotton and light facial mask. And (5) standby.

Test example 4: micro-ecological test of facial skin

1) Volunteer enrollment criteria and grouping

Volunteers were all from college students in school, aged 20-25 years. The facial skin of the subject needs to be healthy and free of any disease. Within 7 days, the skin surface of the subject cannot be smeared with any antibiotic or hormone product, and no antibiotic or antibacterial drug is taken orally within one month. Subjects read and sign informed consent. A total of 100 individuals were selected, and groups A to E (5 groups) were randomly included in 20 individuals. Panel a used the sample of example 1; group B used the example 2 sample; group C used the sample of example 29; panel D used example 30 sample; group E used the example 61 sample.

2) Sample collection

Early preparation: volunteers were not allowed to use any cosmetics and makeup 5 days before testing.

The test process comprises the following steps: subjects were not asked to perform facial cleansing and apply any makeup 8 hours before sampling; the test time was three weeks, and the volunteers used the assigned example mask once a day (the mask was applied by applying the example mask to the face position and spraying 20ml of purified water to uniformly apply the mask to the skin), and no other cosmetics or color cosmetics were allowed. Facial skin microorganisms were sampled before and after application of the mask to volunteers. The sampling method consisted of dipping two sterile cotton swabs with a wetting solution (0.9% NaCl-0.1% Tween 20), wiping 10 times in each area of bilateral symmetry of the cheek skin of the subject, while taking care to rotate the cotton swabs for about 5s, and then placing the cotton swabs into a collection tube. After collection, the samples were stored in dry ice and subsequently transferred to-80 ℃ and the DNA extracted as soon as possible.

3) DNA extraction and PCR amplification

The present invention adopts DNeasy Blood of Qigen&The Tissue Kit extracts the total DNA of the skin surface bacteria. The DNA concentration and purity were checked by NanoDrop2000, and the DNA extraction quality was checked by 1% agarose gel electrophoresis. The V1-V2 variable region was PCR amplified with 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 338R (5'-TGCTGCCTCCCGTAGGAGT-3') primers (the target region genome for PCR amplification of fungi was Internal Transcribed Spacer 1 region (ITS1), ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS2R (5'-GCTGCGTTCTTCATCGATGC-3')), and the amplification program was: pre-denaturation at 95 ℃ for 3min,27 cycles (denaturation at 95 ℃ for 30s, annealing at 55 ℃ for 30s, extension at 72 ℃ for 45 s), and final extension at 72 ℃ for 10min (PCR apparatus: ABI)

Model 9700). The amplification system was 2ul, 4ul 5 × FastPfu buffer, 2ul 2.5mM dNTPs,0.8ul primer (5 uM), 0.4ul FastPfu polymerase; 10ng of DNA template.

4) Illumina Miseq sequencing

The PCR product was recovered using a 2% agarose Gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, union City, calif., USA), eluted with Tris-HCl, and detected by 2% agarose electrophoresis. Utilizing QuantiFluor ^TM Assay quantification was performed by ST (Promega, USA). The purified amplified fragments were used to construct a library of PE 2 x 300 according to the standard protocol of the Illumina MiSeq platform (Illumina, san Diego, USA). Constructing a library, namely (1) connecting a Y-shaped joint; (2) removing the adaptor self-ligating fragment using magnetic bead screening; (3) enriching the library template by utilizing PCR amplification; (4) sodium hydroxide denaturation to generate single-stranded DNA fragments. Sequencing was performed using the Miseq PE300 platform from Illumina (Megi biomedical science and technology, inc., shanghai).

5) Data processing

The original sequencing sequence is subjected to quality control by using Trimmomatic software, and spliced by using FLASH software: (1) Setting a 50bp window, if the average mass value in the window is lower than 20, cutting off a rear-end base from the window, and removing a sequence with the length lower than 50bp after quality control; (2) The barcode needs to be matched accurately, the primer allows the mismatching of 2 bases, and fuzzy bases are removed; (3) And splicing the sequences at two ends according to the overlapping base overlap, wherein the overlap needs to be more than 10bp. And removing sequences which cannot be spliced.

The sequence was OTU clustered according to 97% similarity using UPARSE software (version 7.1http:// drive5.Com/UPARSE /); chimeras were removed using UCHIME software. Each sequence was annotated with a species classification using RDP classifier (http:// RDP. Cme. Msu. Edu /), and aligned to the Silva database (SSU 123) setting the alignment threshold at 70%.

6) Statistical analysis

Paired T-tests were used between the two groups, with P <0.05 indicating that the differences were statistically significant. At a similarity level of 97%, the samples were clustered and annotated, yielding a total of 3329 OTUs (Operational Taxomic Units) belonging to 30 phyla, 962 genera, 1975 species for 42 samples. Alpha diversity analysis was performed on both groups of samples before and after mask use. The Coverage index for all samples in this study was greater than 0.99, indicating that this sequencing covers all species of the sample. The 5 groups of masks were analyzed for differences between groups using SPSS software for Shannon (fig. 3 (a)) and Chao1 (fig. 3 (b)) indices before and after use. Figure 3 (a) shows the variation of the Shannon index of diversity (OTU level) between groups before and after mask use. The Shannon index is used to assess the abundance and uniformity of species composition in a sample. A larger index indicates a richer variety of species and a higher biodiversity for the ecology. Conversely, the lower the Shannon index, the lower the diversity of the skin microecological flora. Figure 3 (b) shows the diversity Chao1 index comparison (OTU levels) between groups before and after mask use. The Chao1 index estimates the index of the number of OTUs contained in a sample, with larger numbers representing more species contained in the sample. The results show that the diversity and the abundance of facial skin bacteria of the facial mask B and the facial mask E are obviously changed after the facial mask B and the facial mask E are used.

Diversity analysis is an important component of the diversity of biological microorganisms and is also a part of the basis of comparison. Alpha diversity refers to the diversity in a specific area or ecosystem and is a comprehensive index reflecting the richness and uniformity. A. TheThe alpha diversity is mainly related to two factors, one is the number of species, i.e. the richness; second, diversity, uniformity in individual distribution in the community. The community diversity index is mainly embodied by Shannon index, and the community abundance index mainly comprises a Chao1 index. As can be seen from fig. 3 (a), diversity Shannon index between B mask and E mask significantly changed before and after mask use, wherein Shannon index of B mask significantly increased, indicating that the facial micro-ecological diversity was significantly improved after 3 weeks of continuous use of the mask. The facial skin micro-ecology maintains good diversity distribution, namely the balance of micro-ecological microbial communities is the basis of skin health. Skin problems begin with a disruption in the balance and diversity of the skin's micro-ecological microflora, i.e. one or several microorganisms become dominant, such as the occurrence of facial acne, due to a high correlation of an unbalanced excessive proliferation of propionibacterium acnes; the development of atopic skin inflammation, superficially rough, dry, barrier-breaking, is actually associated with the excessive proliferation of staphylococcus aureus. The overgrowth of Candida albicans in the closed environment of diaper rash causes the number and diversity of other healthy flora to be inhibited on the skin surface. In addition, for example, the nocturnal odor is associated with diphtheria-like bacillus, the dandruff is associated with pityrosporum orbiculare, and the like. Human skin surface colonizing human body with a large number of microorganisms (10) ⁶ Per cm ² ) And these microorganisms and the different niches of the skin surface form a complex ecosystem-the skin micro-ecology. The flora on the skin surface is divided into resident flora and temporary flora, and most of the flora is beneficial to our bodies, such as cleaning garbage (apoptotic cells) on the skin surface, decomposing redundant lipid, resisting invasion of external pathogenic bacteria through competitive inhibition, and the like. When the balance and diversity relationship is broken, the microecosystem, which is the first barrier of human skin, is likely to be attacked by opportunistic pathogens, causing a variety of infectious diseases and skin problems, and the skin functions are disturbed. The abundance of skin microecological florae of the B facial mask after use can be obviously improved from the Chao1 index and is improved from about 500OTUs to about 600 OTUs, so that the diversity and the abundance of the florae are greatly enriched, and the health of the skin is improvedKang (health recovery). In the components used by the invention, after the formula composition of the facial mask C and the facial mask D is compared, the combination of panthenol and sericin has a very obvious synergistic effect of promoting the diversity of skin micro-ecological bacteria, and the result shows that although the use of a single component does not cause adverse effects on the diversity and the richness of the skin micro-ecological bacteria, the existing balance of a micro-ecological system is basically maintained, but the skin micro-ecological bacteria can not be positively enriched and diversified. From the view of the composition and the using effect of the B mask, a new solution is undoubtedly developed for the health of the skin and the solution of the skin problem. For specific amino acid combinations and types in sericin, the synergistic panthenol is dexpanthenol which can be converted into vitamin B5 under the oxidation action of microorganisms and enzymes and participate in metabolic reaction processes of cells and microorganisms, such as the metabolism of amino acid, the generation of lipid structures in cell membranes and the like, the acquisition process of microbial energy and the like. Is a very important microbial organism health promoter and a coordinating agent, is combined with sericin in a precise ratio, and is a very excellent skin micro-ecological health promoter.

The results of the comparison of the diversity Chao1 index and Shannon index between groups before and after the use of the inverse E mask showed significant reduction in diversity and richness. The proportion design mainly relates to the addition of the preservative, and the preservative has obvious destructive effect on the health and balance of the skin surface micro-ecology. From the action mechanism of the preservative, the microbial cell wall is destroyed or the formation of the microbial cell wall is inhibited; secondly, the cell membrane of the microorganism is destroyed or the function of the cell membrane is influenced, so that the substance in the microorganism cell leaks to cause death; and thirdly, inhibiting enzyme or protein synthesis or denaturing protein in the microbial cell, resulting in death of the microbe. Therefore, from the human body safety end, the preservative does not cause the problems of irritation, safety and toxicology to the human skin within a certain concentration range, but has the influence of 'fine and silent moisture' and long-term potential destructive effect from the aspects of diversity and richness of microecological flora. The subordinate hydroplanar part of the bacterial composition is mainly propionibacterium and staphylococcus, the abundance of five groups is more than 1%, and 18 dominant genera with classified names exist, and the result is shown in fig. 4, and the proportion reaches 85% of the total amount of skin bacteria. As shown in fig. 4, pseudomonas (Pseudomonas) (0.47 ± 0.52vs 2.18 ± 1.90, p = 0.019) and Xanthomonas (Xanthomonas) (0.06 ± 0.13vs 2.11 ± 2.07, p = 0.006) were significantly increased after the B mask was used. Staphylococcus (27.31 + -18.74vs 17.01 + -11.18, p = 0.028) is significantly reduced after the E facial mask is used, while the B facial mask has no significant change (21.08 + -16.4 vs 19.44 + -15.41, p = 0.829), which indicates that the preservative in the E facial mask inhibits the growth of staphylococcus epidermidis.

Researches show that the staphylococcus epidermidis is an important skin probiotic, and can secrete antibacterial peptide on the surface of the skin and resist the colonization of external pathogenic bacteria and the like on the surface of the skin. And regulating pH value of skin, and caring skin. Some pathogenic bacteria, such as pseudomonas aeruginosa and the like, are easy to colonize in people who are weak, fragile and have reduced diversity of skin micro-ecological bacteria colonies, so that a series of skin inflammation, suppurative diseases and the like are caused.

The skin external preparation is preferably a cosmetic, such as a lotion, essence, cream, etc. The product obtained in the above examples can be used alone or as an additive in an external preparation for skin, and the weight percentage of the product is 0.001-50% (w/w). The preferable weight percentage is 1 to 20 percent (w/w). More preferably 2% to 10% (w/w). The most preferred weight percentage is 3% to 8% (w/w).

The following are examples containing specific applications of the above examples in external preparations for skin, and formulations and preparation methods of these dosage forms. In the tables, "-" indicates no addition.

Application example 1: preparation of face cream

The raw materials and contents are shown in Table 4

TABLE 4

Application example 2: preparation of the emulsion

The raw materials and contents are shown in Table 5.

TABLE 5

Application example 3: preparation of jelly

The raw materials and contents are shown in Table 6.

TABLE 6

Application example 4: preparation of astringent

The raw materials and contents are shown in Table 7

TABLE 7

Application example 5: preparation of essence

The raw materials and contents are shown in Table 8.

TABLE 8

Application example 6: preparation of facial mask

The raw materials and contents are shown in Table 9

TABLE 9

Application example 7: preparation of eye cream

The raw materials and contents are shown in Table 10.

Watch 10

Application example 8: preparation of shower gel

The raw materials and contents are shown in Table 11.

TABLE 11

Application example 9: preparation of facial cleanser

The raw materials and contents are shown in Table 12.

TABLE 12

Examples 1 to 30 in the present invention can be used alone as a low-temperature freeze-dried product.

The cosmetic prepared by using the example product of the invention achieves the using effect equivalent to or better than that of the commercial product.

The present invention is illustrated in detail by the examples described above, but the present invention is not limited to the details described above, i.e., it is not intended that the present invention be implemented by relying on the details described above. It should be understood by those skilled in the art that any modifications of the present invention, equivalent substitutions of the raw materials of the product of the present invention, and the addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims (10)

1. A composition for regulating and controlling skin microecological diversity comprises hydroxyethyl urea, tremella fruiting body, panthenol and hydrolyzed sericin.

2. The composition of claim 1, wherein the ratio of hydroxyethyl urea: tremella sporophore: panthenol: the weight ratio of the hydrolyzed sericin is 0.1-10:0.1-3:0.1-4:0.1-1.

3. The composition of claim 1, wherein the ratio of hydroxyethyl urea: tremella sporophore: panthenol: the weight ratio of the hydrolyzed sericin is 6:1:0.1-4:0.1-1.

4. The composition of claim 1, wherein the composition is in the form of a mask.

5. The composition of claim 1, wherein the composition is in lyophilized form.

6. The composition of claim 5, wherein the lyophilized form is formulated as a tablet, pill, or floe.

7. The composition of any one of claims 1-6, wherein the composition is preservative-free.

8. Use of the composition of any one of claims 1 to 7 for the preparation of an external preparation for skin for regulating the microecological diversity of skin.

9. The use of claim 8, wherein the external skin agent is selected from the group consisting of: lotion, cream and toner.

10. The use according to claim 8, wherein the external preparation for skin comprises 0.001 to 50% by weight of the composition.

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