CN115606601B

CN115606601B - Pesticide composition, pesticide fertilizer, and preparation method and application thereof

Info

Publication number: CN115606601B
Application number: CN202211344623.1A
Authority: CN
Inventors: 何其明; 龚宝金; 陈庭倬; 杜波; 唐青青; 周海伟; 王艳玲; 赵文娟; 任丹
Original assignee: Chengdu New Chaoyang Crop Science Co ltd
Current assignee: Chengdu New Chaoyang Crop Science Co ltd
Priority date: 2022-10-31
Filing date: 2022-10-31
Publication date: 2023-12-08
Anticipated expiration: 2042-10-31
Also published as: CN115606601A

Abstract

The invention discloses a pesticide composition, a pesticide fertilizer, a preparation method and application thereof, wherein the pesticide composition comprises chlorhexidine acetate and amino-oligosaccharin with the weight ratio of 1:99-99:1. The chlorhexidine acetate and the amino-oligosaccharin have obvious synergistic effect, play a good role in preventing and controlling plant related diseases and insect pests, and particularly have good effects in preventing and controlling root knot nematodes, rhizoctonia solani, eggplant root rot and bacterial wilt, and are worthy of popularization and application. Meanwhile, the problem of drug resistance caused by long-time use of the existing chemical reagent is solved.

Description

Pesticide composition, pesticide fertilizer, and preparation method and application thereof

Technical Field

The invention relates to the technical field of pesticides, in particular to a pesticide composition, a pesticide fertilizer, a preparation method and application thereof.

Background

In the actual process of agricultural production, the economic crops are planted, so that the plant diseases and insect pests can be effectively prevented and treated, and meanwhile, the quality is high, the yield is high, and the soil is healthy. For a long time, the reasons why the economic crops such as ginger, tomato, watermelon, celery and the like cannot grow healthily due to the diseases and insect pests such as rhizoctonia solani, eggplant root rot and the like are mainly that the chemical pesticides are used in a large amount to increase the resistance, the diseases and insect pests are difficult to control, the cost is increased, the pesticide residues exceed standards, the quality is reduced and the like.

Disclosure of Invention

The invention aims to provide a pesticide composition, which solves the problems of increased resistance, difficult pest control, increased cost, excessive pesticide residue and reduced quality caused by the large-scale use of chemical pesticides in the existing chemical control for treating the rhizoctonia solani, the root rot of eggplant and the bacterial wilt.

In a first aspect, the invention provides a pesticide composition comprising chlorhexidine acetate and amino-oligosaccharin in a weight ratio of 1:99 to 99:1.

Under the condition of adopting the technical scheme, the chlorhexidine acetate not only can destroy the cell wall plasma membrane of pathogenic microorganisms and quickly kill gram-positive bacteria, gram-negative bacteria, bacterial spores, fungi, viruses and the like, but also has a quick permeation effect, so that the chlorhexidine acetate can promote the absorption and conduction of plants to the amino-oligosaccharin, so that the amino-oligosaccharin acts on nematode cell membranes and destroys the cell wall plasma membrane of the pathogenic microorganisms and quickly kills nematodes and pathogenic bacteria.

Because the amino oligosaccharin has strong rooting and root nourishing effects, a protective film can be formed on the surface of a root system, the root system is protected from being infected by nematodes and germs, and meanwhile, the produced chitinase directly acts on the nematodes and inhibits the growth of mycelium, and meanwhile, the growth of root system cells is promoted, so that capillary roots are rapidly increased, the occurrence of retting, dead roots and rotten roots is reduced, the root system is promoted to develop, the plant growth is facilitated, and nutrients are timely absorbed from soil.

The pesticide composition has good effect in treating plant-related diseases and insect pests, in particular root-knot nematodes, rhizoctonia solani, eggplant root rot and bacterial wilt.

As a preferred embodiment, the pesticide composition comprises chlorhexidine acetate and amino-oligosaccharin in a weight ratio of 10:1-30:1.

As a preferred embodiment, the pesticide composition comprises chlorhexidine acetate and amino-oligosaccharin in a weight ratio of 20:1.

In a second aspect, the invention also provides a pesticide fertilizer, which is characterized by comprising the pesticide composition, a surfactant, a nutrient substance, an antifoaming agent and a stabilizer.

As a possible implementation manner, the dosage form of the medical fertilizer is liquid or solid.

As a possible implementation manner, when the dosage form of the medical fertilizer is liquid, the medical fertilizer comprises the following components in percentage by mass:

0.1 to 50 percent of the pesticide composition, 0.5 to 10 percent of surfactant, 5 to 40 percent of solvent, 5 to 40 percent of nutrient substances, 0.01 to 0.2 percent of defoamer, 0.1 to 1 percent of preservative, 0.1 to 5 percent of stabilizer and the balance of water. As a possible implementation manner, when the dosage form of the medical fertilizer is solid, the medical fertilizer comprises the following components in percentage by mass:

0.1 to 50 percent of the pesticide composition, 0.5 to 5 percent of surfactant, 10 to 50 percent of nutrient substances, 0.1 to 0.2 percent of defoamer, 0.1 to 5 percent of stabilizer and the balance of solid filler. In a third aspect, the invention also provides a preparation method of the medical fertilizer, which comprises the following steps: mixing water, amino oligosaccharin and solvent, adding nutrient substances, preservative and stabilizer, dissolving, adding chlorhexidine acetate, surfactant and defoamer, and mixing.

The invention has the beneficial effects that: the medical fertilizer disclosed by the invention has the characteristics of flexible use (can simultaneously meet various farm operations such as spray irrigation, drip irrigation, flushing and the like when being liquid, can meet the requirements of broadcast application, flushing application, ditch application, hole application, soil mixing or use with a base fertilizer when being solid, and the like, is flexibly used according to plant diseases and insect pests), and has good treatment or prevention effects on plant related plant diseases and insect pests, in particular to root knot nematodes, rhizoctonia solani, eggplant root rot and bacterial wilt.

Detailed Description

In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the invention is further described in detail below. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.

The invention aims at solving the problems of difficult control of diseases and insect pests, increased cost, excessive pesticide residue, reduced quality and the like caused by serious drug resistance of the existing chemical pesticide after long-time use, and is particularly suitable for treating the wilt pit, the eggplant root rot and the bacterial wilt.

Aiming at the problems, the inventor adopts the amino-oligosaccharin and chlorhexidine acetate for compound use, not only solves the problems of difficult pest control/cost increase/pesticide residue overscale/quality reduction and the like caused by serious drug resistance of the existing chemical drugs for treating the wilt core, the eggplant root rot and the wilt, but also has obvious synergistic effect on the treatment effects of the wilt core, the eggplant root rot and the wilt due to the single use effects of the two.

In a first aspect, the present invention discloses a pesticide composition comprising an amino-oligosaccharide and chlorhexidine acetate.

The amino-oligosaccharin has the following characteristics: 1. after the soil is applied, a large amount of beneficial bacteria such as actinomycetes and the like can be cultured in a short time, and a large amount of antibiotic substances and resistant enzymes (chitinase and the like) secreted by the beneficial bacteria can directly inhibit nematodes and ova, so that the walls and egg shells of the nematodes are dissolved; 2. the amino-oligosaccharin has strong rooting and root nourishing effects, can form a protective film on the surface of a root system, and simultaneously promote the growth of root system cells, so that capillary roots are rapidly increased, the occurrence of retting, dead roots and rotten roots is reduced, and the root system is promoted to develop; 3. the amino oligosaccharin can stimulate a large amount of antibiotic substances and resistant enzymes (chitinase and the like) secreted by beneficial bacteria after being used, and the substances can inhibit harmful bacteria such as saprophytic fungi, phytophthora, rhizoctonia, fusarium oxysporum, mould, fusarium and the like, so that the complex infection of harmful bacteria caused by nematode harm is reduced.

The chlorhexidine acetate has good broad-spectrum antibacterial and bactericidal effects and low toxicity, and the main action mechanism is to destroy the plasma membrane of the cell wall of pathogenic microorganisms, quickly kill gram-positive bacteria, gram-negative bacteria, bacterial spores, fungi, viruses and the like, and simultaneously has quick permeation effect, and promote the absorption and conduction of liquid medicine.

When chlorhexidine acetate and amino-oligosaccharin are compounded for use, the compound has obvious synergistic effect, and the specific steps are as follows: 1. the chlorhexidine acetate has the advantages of rapid infiltration, capability of promoting absorption and conduction of liquid medicine, capability of rapidly entering plant tissues, direct acting on nematodes and pathogenic microorganisms, and capability of rapidly destroying nematode cell membranes and pathogenic microorganism cell wall plasma membranes, thereby achieving rapid killing of nematodes and pathogenic bacteria. 2. The amino oligosaccharin has powerful rooting and rooting function and can form protecting film on the root system surface to protect crop from exogenous attack.

In general, in the pesticide composition, the weight ratio of the amino-oligosaccharin to the chlorhexidine acetate is 1-99:99-1; more suitably, the weight ratio of the amino-oligosaccharin to the chlorhexidine acetate is 1:10-30; most suitably 1:20.

The pesticide composition can be prepared into a liquid preparation or a solid preparation in a specific use process, and auxiliary reagents such as a surfactant, an antifoaming agent, a stabilizer and the like can be added into the pesticide composition for better use. When the solid auxiliary agent is prepared, the solid auxiliary agent can be applied to the soil, or used together with the base fertilizer, and the specific application mode can be flexibly used according to the occurrence of diseases and insect pests.

In order to avoid the problems of enrichment in soil, low effective utilization rate of nutrients, deterioration of soil quality and continuous cropping obstacle caused by excessive nutrient utilization when the chemical fertilizer is singly used, the invention can add the nutrient substances into the pesticide composition, thereby not only avoiding the problems caused by excessive application of the chemical fertilizer, but also providing nutrients for plants in time so that the amino-oligosaccharin can fully play a rooting role and promote root cell meristem, and further enhance the plant resistance to diseases and insect pests.

Based on the above objects, the present invention provides a pesticide fertilizer including, but not limited to, the aforementioned pesticide composition, surfactants, nutrients, defoamers and stabilizers.

In practical application, the fertilizer can be selected from liquid preparations or solid preparations. The liquid preparation can simultaneously satisfy various agricultural operations such as sprinkling irrigation, drip irrigation, and the like, and the solid preparation can simultaneously satisfy broadcasting, flushing, ditch application, hole application, soil mixing or be used together with base fertilizer, and the like. The dosage form can be flexibly selected according to the occurrence of plant diseases and insect pests in actual use.

When the medical fertilizer is a liquid preparation, the medical fertilizer comprises the following components in percentage by mass:

0.1 to 50 percent of the pesticide composition, 0.5 to 10 percent of surfactant, 5 to 40 percent of solvent, 5 to 40 percent of nutrient substances, 0.01 to 0.2 percent of defoamer, 0.1 to 1 percent of preservative, 0.1 to 5 percent of stabilizer and the balance of water.

In this liquid formulation, as a preferred embodiment, the aforementioned pesticide composition includes, but is not limited to, the following components in mass percent:

8 to 13 percent of the pesticide composition, 5 to 10 percent of surfactant, 5 to 15 percent of solvent, 30 to 40 percent of nutrient substance, 0.01 to 0.1 percent of defoamer, 0.1 to 1 percent of preservative, 0.1 to 3 percent of stabilizer and the balance of water.

In the present invention, the surfactant may be selected from a single agent or a complex formulation of a common nonionic surfactant or an anionic surfactant or a cationic surfactant or a zwitterionic surfactant. For example: anionic surfactants (sodium dodecylbenzenesulfonate, sodium alkylpolyoxyethylene ether sulfate, sodium alkylpolyoxyethylene ether carboxylate, etc.), and cationic surfactants (dodecylammonium bromide, tetradecylammonium bromide, etc.).

In the present invention, the solvent may be at least one selected from the group consisting of N, N-dimethylformamide, methanol, ethanol, ethylene glycol, propylene glycol, N-butanol, ethylene glycol butyl ether, acetone, cyclohexanone, N-methylpyrrolidone, ethyl acetate, dimethylacetamide, triethanolamine, acetonitrile, isopropylamine, deionized water, and the like.

In the present invention, the nutrient may be at least one selected from nitrogen, phosphorus, potassium, medium trace elements, amino acids, humic acid, proteins, and the like.

In the present invention, the defoaming agent may be selected from silicone defoaming agents, polyether defoaming agents, and the like.

In the present invention, the preservative may be selected from sodium benzoate, potassium sorbate, and the like.

In the present invention, the stabilizer may be at least one selected from epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, or triphenyl phosphate.

The preparation method of the liquid preparation can include, but is not limited to, the following steps:

mixing water, amino oligosaccharin and solvent, adding nutrient substances, preservative and stabilizer, dissolving, adding chlorhexidine acetate, surfactant and defoamer, and mixing.

When the medical fertilizer is a solid preparation, the medical fertilizer comprises the following components in percentage by mass:

0.1 to 50 percent of the pesticide composition, 0.5 to 5 percent of surfactant, 10 to 50 percent of nutrient substances, 0.1 to 0.2 percent of defoamer, 0.1 to 5 percent of stabilizer and the balance of solid filler.

In this solid state formulation, as a preferred embodiment, the aforementioned pesticide composition includes, but is not limited to, the following components in mass percent:

8 to 13 percent of the pesticide composition, 3 to 5 percent of surfactant, 30 to 50 percent of nutrient substances, 0.1 to 0.2 percent of defoamer, 0.1 to 5 percent of stabilizer and the balance of solid filler.

In the present invention, the optional specific types of the surfactant and the nutrient are the same as those of the surfactant and the nutrient, and are not described herein.

In the present invention, the defoaming agent may be a silicone defoaming agent, and the stabilizer may be selected from BHT, pyrophosphate, and the like.

In the present invention, the solid filler may be at least one selected from kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch, light calcium carbonate, urea, potassium sulfate, ammonium sulfate, and the like.

The preparation method of the aforementioned solid preparation may include, but is not limited to, the following steps:

mixing the raw materials, and grinding to the required mesh number. Typically, the mesh number is greater than 100.

The pesticide composition and the pesticide fertilizer disclosed by the invention have good prevention effect on plant-related diseases and insect pests, in particular to root-knot nematodes, rhizoctonia solani, eggplant root rot and bacterial wilt, and are worthy of popularization and application.

Examples

In the following examples and comparative examples, in order to illustrate that the amino oligosaccharin and chlorhexidine acetate can be smoothly administered after being compounded, an emulsifier and a solvent which do not significantly promote their effects are added during the preparation, wherein the emulsifier may be selected from at least one of OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate salt, magnesium alkylbiphenyl ether disulfonate, triethanolamine salt, agro-400 # (benzyldimethyl phenol polyoxyethylene ether), agro-700 # (alkylphenol-formaldehyde resin polyoxyethylene ether), ning-36 # (phenethyl phenol formaldehyde resin polyoxyethylene ether), agro-1600 # (phenethyl phenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), agro-33 (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitan monostearate), tween series (sorbitol polyoxyethylene ether) or o (fatty alcohol polyoxyethylene ether), and alkyl glycoside series (ae-0). The specific type of solvent that may be selected is the same as the specific type of solvent described above and will not be described again here.

Example 1

This example discloses a pesticidal composition which is a liquid formulation and whose composition is shown in table 1.

TABLE 1

Component (A)	Quality (g)
		Amino oligosaccharins	0.5
Chlorhexidine acetate	10
		Alkyl glycoside 0810 (emulsifier)	5
95% ethanol (solvent)	10
		Deionized water	74.5

Comparative example 1

This comparative example discloses a pesticide whose composition is shown in table 2.

TABLE 2

Comparative example 2

This comparative example discloses a pesticide whose composition is shown in table 3.

TABLE 3 Table 3

Component (A)	Quality (g)
		Chlorhexidine acetate	10
Alkyl glycoside 0810 (emulsifier)	5
		95% ethanol (solvent)	10
Deionized water	75

Example 2

This example discloses a pesticidal composition which is a liquid formulation and whose composition is shown in table 4.

TABLE 4 Table 4

Component (A)	Quality (g)
		Amino oligosaccharins	10
Chlorhexidine acetate	0.5
		Alkyl glycoside 0810 (emulsifier)	5
95% ethanol (solvent)	10
		Deionized water	74.5

Example 3

This example discloses a pesticidal composition which is a liquid formulation and whose composition is shown in table 5.

TABLE 5

Component (A)	Quality (g)
		Amino oligosaccharins	10
Chlorhexidine acetate	40
		Alkyl glycoside 0810 (emulsifier)	5
95% ethanol (solvent)	10
		Deionized water	35

Example 4

This example discloses a pesticidal composition which is a liquid formulation and whose composition is shown in table 6.

TABLE 6

Component (A)	Quality (g)
		Amino oligosaccharins	40
Chlorhexidine acetate	10
		Alkyl glycoside 0810 (emulsifier)	5
95% ethanol (solvent)	10
		Deionized water	35

Example 5

This example discloses a pesticidal composition which is a liquid formulation and whose composition is shown in table 7.

TABLE 7

Component (A)	Quality (g)
		Amino oligosaccharins	0.5
Chlorhexidine acetate	49.5
		Alkyl glycoside 0810 (emulsifier)	5
95% ethanol (solvent)	10
		Deionized water	35

Example 6

This example discloses a pesticidal composition which is a liquid formulation and whose composition is shown in table 8.

TABLE 8

Component (A)	Quality (g)
		Amino oligosaccharins	49.5
Chlorhexidine acetate	0.5
		Alkyl glycoside 0810 (emulsifier)	5
95% ethanol (solvent)	10
		Deionized water	35

The pesticides of example 1 and comparative examples 1-2 were tested for root knot nematode, rhizoctonia solani and eggplant root rot control.

1. Root knot nematode control experiment

1. Test target:

tomato and cucumber root knot nematode (insect source comes from Shandong Weifang)

2. Test agent:

in example 1, the amino-oligosaccharin stock of comparative examples 1 and 2 was purchased from biotechnology limited, chang, shandong, and chlorhexidine acetate was purchased from the pharmaceutical industry limited, chang, zhejiang.

3. Chemical control: 5% abamectin (Mingjian) dosage form: emulsifiable concentrate pesticide registration number: PD20173106 lot number: 20200421041 shelf life: for 2 years, weilda chemical Co., ltd. In Zhejiang.

4. And (3) clear water control: distilled water (sterile pure water)

5. Instrument apparatus:

dissecting mirrors, pointed forceps, cross-hatching culture dishes, 5ml centrifuge tubes, beakers, pipette guns, etc.

6. The experimental method comprises the following steps:

1) Preparation of nematode solution

Picking root knot nematode egg mass, taking brown or milky oval dots on the surface of root system of plant (tomato or cucumber), peeling with forceps, placing into a beaker containing 30ml of pure water (filter screen is placed in the beaker), sealing with filter paper, and culturing in a culture room at 25deg.C for 48 hr to obtain second-instar larva with consistent instar.

Preparing a nematode suspension, taking 1ml of uniformly mixed liquid containing root-knot nematodes into a counting plate, and counting under a dissecting mirror to ensure that the nematode suspension volume per ml is more than or equal to 100 nematodes. The method has the advantages that the nymphs are small, the centrifugation operation is also needed, the centrifugation is carried out for 2 minutes at 1000r/min, the supernatant is discarded, the centrifugation is carried out again, and finally, the nematodes are resuspended to more than or equal to 100 per milliliter by clear water for standby.

2) Treatment method

Adopts an insect immersing method. Adding 2ml of the prepared medicament into a 5ml centrifuge tube, adding 2ml of prepared nematode suspension, uniformly mixing the liquid medicine with nematodes (1:1), capping, and culturing at a constant temperature of 25 ℃.

7. Investigation of results

7-1 investigation time and method

After 48 hours, 1ml of mixed solution is taken from each treatment, the death condition of nematodes is observed under a body vision mirror, the number of nematodes is not less than 50 per repeated observation, and the number of bus worms and the number of dead nematodes are recorded. The commonly dead nematodes are stiff and do not move in flexion.

Note that: the treatment group with more dead insects (the dead insects number is more than 50%), the treatment group needs to be resuscitated by adding clear water for more than 2 hours after the first investigation, whether the nematodes are resuscitated and are re-activated is observed, and the second investigation data are taken as the final test result.

7-2 calculation method

Based on the survey data, the control effect of each treatment was calculated. And (3) calculating according to the formulas (1) and (2), wherein the calculation results are reserved to the last two decimal places.

Mortality (%) = number of dead nematodes/number of total nematodes investigated ×100

Corrected mortality (%) = (treated nematode mortality-control nematode mortality)/(100-control nematode mortality) ×100

7-3 test results and analysis

Based on the test results, the control effect of each sample on the root-knot nematode was analyzed, and the results are shown in Table 9.

TABLE 9

As shown in Table 9, the amino-oligosaccharin single agent (comparative example 1) has a certain control effect on nematodes, but the control effect is poor at all concentrations set in the test, the chlorohexidine acetate single agent (comparative example 2) has an inhibition effect on nematodes at a high concentration 1000 times in the test, the control effect reaches about 52%, but when the amino-oligosaccharin and the chlorohexidine acetate are compounded (example 1), the control effect reaches 100% at concentrations 1000 times and 2000 times, the control effect is obviously better than the effects of the amino-oligosaccharin single agent (7.19%, 0.73%) and the chlorohexidine acetate single agent (52.33%, 19.97%), and the obvious synergistic effect is shown after the amino-oligosaccharin and the chlorohexidine acetate are compounded, so that the control effect on nematodes is obviously improved.

Compared with the existing common reagent for preventing and treating nematodes, 5% avermectin has good effect (95.76%) at the same concentration, but does not achieve 100% prevention effect after the amino oligosaccharin and chlorhexidine acetate are compounded, the probability of recurrence after stopping using 5% avermectin is high, and recurrence hardly occurs after the two are compounded.

2. Test of prevention effect of rhizoctonia solani and root rot of eggplant

2-1 test strains

146 rhizoctonia solani and 77 eggplant root rot

2-2 test agent

2-3 control Agents

98% hymexazol soluble powder (Tianjin Lv Heng chemical Co., ltd., pesticide registration number: PD20190146, date of production: 20211008)

2-4 instruments and equipment

Vertical steam sterilizer (YAIB, shanghai BoXie medical equipment factory), hot air sterilizing box (GR-140, shanghai BoXie medical equipment factory), electronic balance (sensing 10 mg), inoculating needle, culture dish, pipette-lab, etc.

2-5. Test preparation:

PDA culture medium was prepared: prepared with potato dextrose agar medium (manufactured by Qingdao high tech Industrial park Haibo biotechnology Co., ltd.) and added in an amount of 46 g/1000 ml water.

2-6, strain activation and inoculation propagation: inoculating the frozen strain to the center of PDA culture medium on an ultra-clean workbench, marking, culturing in an incubator, and propagating.

10ml centrifuge tube with cover, pipette tip, deionized water, triangular flask, etc. are sterilized by heat and humidity at 121deg.C for 20min for use.

2-7, dispensing:

the preparation of the medicament is carried out on an aseptic working table, the whole process is aseptic, and all glassware and pipette tips are strictly sterilized. When the liquid medicine is added into the liquid medicine transferring gun, the gun head is replaced once, and the liquid medicine transferring gun is not reused.

The preparation method comprises the steps of preparing the medicaments according to the test design concentration reduced by 10 times, measuring each medicament according to the calculated amount, and then using sterilized water to fix the volume to 10ml, and shaking uniformly for standby.

2-8, pouring the culture medium:

heating and dissolving the prepared PDA culture medium, and cooling to 45-50 ℃ for standby;

taking 1ml of the prepared liquid medicine, putting the liquid medicine into a culture dish, adding 9ml of the PDA culture medium, shaking uniformly, marking, horizontally placing and cooling for 30min for later use.

2-9, inoculation:

and (3) punching holes in the culture medium successfully propagated by using a puncher according to concentric circumferences. Then inoculating the strain on the surface of the culture medium, and culturing the strain in an incubator at 26 ℃.

2-10. Investigation of results:

2-10-1 investigation time and method

When two fungus circles of the blank hypha grow to be in quick contact, the diameter of a colony is measured, the cross method is used for measuring, the average diameter is calculated, and the bacteriostasis rate of the medicament is calculated. When the antibacterial rate is greater than 80%, a second observation is needed to know the persistence of the medicament.

2-10-2. Calculation method

The calculation formula of the bacteriostasis rate: i= [ D0-Dt)/(D0-4) ] 100%

I: hypha growth inhibition rate

D0: diameter of blank colony

Dt: diameter of colony treated with agent

Table 10

As can be seen from Table 10, the amino-oligosaccharin single agent (comparative example 1) has poor control effect on the core of the withered wire and the root rot of the eggplant, the chlorhexidine acetate single agent (comparative example 2) has an inhibition effect on the core of the withered wire at the concentration tested at the time, but after the amino-oligosaccharin and the chlorhexidine acetate are compounded (example 1), under the same using multiple, the control effect after the compounding is obviously superior to the effect of the core of the withered wire and the root rot of the eggplant, and the compound use of the two has obvious synergistic effect.

Compared with the existing 98% hymexazol soluble powder used for preventing and treating the rhizoctonia solani and the root rot of the eggplant, the effect of the soluble powder is better than that of the 98% hymexazol soluble powder in preventing and treating the rhizoctonia solani, so that the soluble powder of 98% hymexazol or the soluble powder of the two can be replaced by the amino-oligosaccharin and the chlorhexidine acetate in combination when preventing and treating the diseases, and the occurrence of drug resistance is avoided. In the prevention and treatment of the root rot of eggplant, the compound of the amino-oligosaccharin with higher dilution multiple and chlorhexidine acetate can be adopted to replace 98% of hymexazol soluble powder or alternately use the amino-oligosaccharin and the chlorhexidine acetate, so that the occurrence of drug resistance is avoided.

3. Bacterial wilt prevention effect test

3-1 test strains

Bacterial wilt (Ralstonia solanacearum) is obtained from Sichuan shi 370225, and is identified by Beijing Qingke biological component company.

3-2 test agent

3-3 control Agents

3% Zhongshengmycin solution (Fujian Kaili biological products Co., ltd., pesticide registration number: PD20190224; date of production 2021.03.15)

3-4. Instrumentation

Vertical steam sterilizer (YAIB, shanghai BoXie medical equipment factory), hot air sterilizing tank (GR-140, shanghai BoXie medical equipment factory), atomic absorption spectrophotometer (UV-8000 Shimadzu corporation), electronic balance (sensing amount 10 mg), inoculating needle, culture dish, pipette-lab, etc

3-5 preparation of test

The culture dish is cleaned, dried and sterilized by dry heat (180 ℃ for 60 min) for standby. 10ml sterilizing centrifuge tube, puncher, inoculating needle, pipette (12 for 10ml, 5ml, 1ml each), and wet heat sterilizing (121deg.C, 30 min).

3-6, preparing a liquid culture medium B: weighing 10g of peptone, 1g of yeast, 1g of casein amino acid and 5g of grape, adding water to 1000ml, stirring to obtain uniform solution, packaging into conical flask, sealing, and sterilizing in autoclave (121 ℃) for 20 min.

3-7, strain activation: and (3) at 2022, 7 and 19 days, sucking 1ml of tobacco bacterial wilt frozen in an ultralow temperature refrigerator by using a pipetting gun, adding 20ml of B liquid culture medium, and culturing in a shaking table at a constant temperature of 30 ℃ for 24 hours for later use.

3-8, medicated culture

20 days 7 and 20 days 2022, 20mlB liquid culture medium is measured, 10ul of activated bacterial liquid is placed in a 50ml triangular conical flask, the medicament is measured, the medicament and the control are repeated three times, and the bottle mouth is sealed and then cultured in a constant-temperature oscillating bed at 30 ℃ for 24 hours.

3-9. Measurement of absorbance

The bacterial solutions cultured in the above were measured by a spectrophotometer at 600nm on day 21 and 7 in 2022, and the absorbance of each treated bacterial solution was not more than 3, otherwise, the measurement was performed after dilution, and the medium was used as a reference solution in the measurement. The absorbance of each treated bacterial liquid was recorded.

3-10. Investigation of results

Investigation time and method

And (3) when the bacterial colonies grow out in the culture box until the blank control, counting the number of the bacterial colonies, and calculating the antibacterial rate of the medicament.

The calculation formula of the bacteriostasis rate: i= [ D0-Dt)/D0 ] 100%

I: inhibition rate

D0: blank colony count

Dt: number of colonies treated with the agent

TABLE 11

/>

As shown in Table 11, the amino-oligosaccharin single agent (comparative example 1) has poor prevention effect on bacterial wilt, the chlorhexidine acetate single agent (comparative example 2) has good inhibition effect on bacterial wilt at the concentration tested at the time, and after the amino-oligosaccharin and the chlorhexidine acetate are compounded, the amino-oligosaccharin single agent has good prevention effect on bacterial wilt.

In conclusion, the amino-oligosaccharin and chlorhexidine acetate have obvious synergistic effect after being compounded, have good effects in preventing and treating root knot nematode, rhizoctonia solani, eggplant root rot and bacterial wilt, and are worthy of popularization and application.

Similarly, after being compounded with other components such as nutrient substances, emulsifying agents, surfactants, defoamers, preservatives, stabilizers or solid fillers, the amino oligosaccharins and the chlorhexidine acetate are prepared into the medical fertilizer, and as the components exert their respective effects, the prepared medical fertilizer also has corresponding control effects on the diseases and insect pests.

The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.

Claims

1. The pesticide composition is characterized by comprising chlorhexidine acetate and amino-oligosaccharin in a weight ratio of 20:1.

2. A pharmaceutical fertilizer comprising the pesticidal composition of claim 1, a surfactant, a nutrient, an antifoaming agent, and a stabilizer.

3. The medical fertilizer according to claim 2, wherein the medical fertilizer is in a liquid or solid form.

4. A medical fertilizer according to claim 3, wherein when the dosage form of the medical fertilizer is liquid, the medical fertilizer comprises the following components in percentage by mass:

the pesticide composition of claim 1, wherein the pesticide composition comprises 0.1-50% of surfactant, 0.5-10% of solvent, 5-40% of nutrient substances, 0.01-0.2% of defoamer, 0.1-1% of preservative, 0.1-5% of stabilizer and the balance of water.

5. A medical fertilizer according to claim 3, wherein when the dosage form of the medical fertilizer is solid, the medical fertilizer comprises the following components in percentage by mass:

the pesticide composition of claim 1, wherein the pesticide composition comprises 0.1-50% of surfactant, 0.5-5% of nutrient substances, 10-50% of defoamer, 0.1-0.2% of stabilizer and the balance of solid filler.

6. A method of preparing the fertilizer as set forth in claim 4, wherein the method comprises: mixing water, amino oligosaccharin and solvent, adding nutrient substances, preservative and stabilizer, dissolving, adding chlorhexidine acetate, surfactant and defoamer, and mixing.

7. Use of a pesticide fertilizer according to any one of claims 2 to 5 or a pesticide composition according to claim 1 as a medicament in the treatment of plant-related diseases and insect pests.

8. The use according to claim 7, wherein the plant-related pests include root knot nematodes, rhizoctonia solani, solanaceous root rot and bacterial wilt.