CN115541861B - Body fluid sampling and detecting device for neurology department - Google Patents

Body fluid sampling and detecting device for neurology department Download PDF

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CN115541861B
CN115541861B CN202211546333.5A CN202211546333A CN115541861B CN 115541861 B CN115541861 B CN 115541861B CN 202211546333 A CN202211546333 A CN 202211546333A CN 115541861 B CN115541861 B CN 115541861B
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opening
needle
inner bag
sampling
needle tube
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CN115541861A (en
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张付生
张刚
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Shandong Provincial Hospital Affiliated to Shandong First Medical University
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Shandong Provincial Hospital Affiliated to Shandong First Medical University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices

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Abstract

The invention discloses a neurology body fluid sampling and detecting device, relates to the field of detection of auxiliary diagnosis of neurology diseases, and particularly relates to a device for detecting the content of hydrogen sulfide in cerebrospinal fluid, which comprises a sampling component, a reaction component and a measuring component; the sampling assembly is used for quantitatively extracting cerebrospinal fluid; the reaction component provides a reaction space for cerebrospinal fluid and phosphoric acid; the measuring assembly provides a reaction space for hydrogen sulfide and sodium hydroxide, and the sampling assembly comprises a tank body for containing cerebrospinal fluid; the reaction component comprises a box body, the top surface of the box body is communicated with the tank body, so that cerebrospinal fluid in the tank body can flow into the box body; the measuring assembly comprises a bottle body, the bottle body is communicated with the top surface of the box body, and hydrogen sulfide gas can enter the bottle body. The detection device is convenient to use, full in reaction and fine and controllable in reaction process.

Description

Body fluid sampling and detecting device for neurology department
Technical Field
The invention relates to the field of detection of auxiliary diagnosis of neurology diseases, in particular to a device for detecting the content of hydrogen sulfide in cerebrospinal fluid.
Background
The content of hydrogen sulfide in cerebrospinal fluid is a reference basis for diagnosing various diseases, such as Down syndrome, alzheimer disease and the like. In the existing detection process, the fluctuation of the detection result of the hydrogen sulfide is large due to the interference of other components of cerebrospinal fluid, and the accuracy degree of the result is not good. In the prior art, CN110646599B is a device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples and a using method thereof, two injectors and a centrifugal tank are vertically communicated. The injector at the bottom injects cerebrospinal fluid into the injector filled with phosphoric acid in the middle, and hydrogen sulfide is separated out under the action of phosphoric acid. Hydrogen sulfide gas is pushed and injected into the centrifugal tank by the middle injector to react with sodium hydroxide in the centrifugal tank. And finally detecting the content of the hydrogen sulfide. The device structure has the following disadvantages: 1. the device must be vertically arranged, and the phosphoric acid is prevented from entering the centrifugal tank, so that the reaction result is interfered, and the use is very inconvenient; 2. hydrogen sulfide enters a sodium hydroxide solution, sufficient reaction time is not available, bubbles float up too fast, and partial hydrogen sulfide cannot react with sodium hydroxide, so that the gas pushing rate influences the detection result to a great extent; 3. the injector injects cerebrospinal fluid, which actually implies the process of pumping cerebrospinal fluid by the injector, and the hydrogen sulfide is volatilized when the cerebrospinal fluid is placed in an open way; 4. the cerebrospinal fluid obtained quantitatively by the injector needs to be completely discharged from the injector before injecting the phosphoric acid, the process is not easy to control, and under the condition that the sample size is not large, when the injector discharges the air, part of liquid is usually discharged to ensure that the air is completely discharged, so that the sample size has random deviation; 5. the middle syringe and the needle are always inserted into the sodium hydroxide at the top, the components in the solution are naturally diffused, and a part of the sodium hydroxide is inevitably reacted with the phosphoric acid, and the reaction amount depends on the operation time.
Disclosure of Invention
The embodiment of the application provides a department of neurology body fluid sampling test device, has solved inconvenient operation among the prior art, and the reaction is incomplete, has the problem of error easily, has realized convenient operation, the abundant effect of reaction.
The embodiment of the application provides a neurology body fluid sampling and detecting device which comprises a sampling assembly, a reaction assembly and a measuring assembly;
the sampling assembly is used for quantitatively extracting cerebrospinal fluid; the reaction component provides a reaction space for cerebrospinal fluid and phosphoric acid; the measuring assembly provides a reaction space for hydrogen sulfide and sodium hydroxide,
the sampling assembly comprises a tank body, and the tank body is used for containing cerebrospinal fluid;
the reaction component comprises a box body, the top surface of the box body is communicated with the tank body, so that cerebrospinal fluid in the tank body can flow into the box body;
the measuring component comprises a bottle body, and the bottle body is communicated with the top surface of the box body, so that hydrogen sulfide gas can enter the bottle body.
Furthermore, the sampling assembly also comprises an inner bag, a filling needle and a sampling nozzle;
the inner bag is a soft bag, one end of the inner bag is communicated with the tail end of a needle tube of the filling needle, and the other end of the inner bag is communicated with the tail end of the sampling nozzle; the filling needle and the sampling nozzle are positioned at two opposite ends of the tank body;
the filling needle comprises a needle tube, an opening and a closed ring;
the top end of the needle tube is a tip end, and the tip end is a closed end; an opening is formed in the needle tube and is close to the bottom edge of the tip end by 2-4 mm; the sealing ring is a rubber ring and is sleeved on the needle tube, and the sealing ring can move on the needle tube under the action of external force; the initial position of the closed ring is sleeved at the opening and can close the opening;
the box body is provided with a liquid injection port, a contact piece is fixed in the liquid injection port, and the contact piece is a rubber piece; the liquid injection port is sealed by the contact piece;
the tank body comprises an installation nozzle and an air faucet;
the tail end of the needle tube is fixedly connected with the tank body, and the needle tube is positioned in the mounting nozzle;
the mounting nozzle can be detachably and fixedly communicated with the liquid injection port; when the mounting mouth is fixed with the liquid injection port, the tip end of the needle tube penetrates through the contact piece, the opening is also positioned below the contact piece, and the closed ring is retained on the upper side of the contact piece;
the air tap is an air charging and discharging port on the tank body.
Further, the tank body further comprises a top cover, and the top cover is detachably and fixedly connected with the opening of the mounting nozzle and used for sealing the opening of the mounting nozzle.
Further, the sampling nozzle is a conical tube.
Further, the conical pipe orifice is provided with a rubber plug.
Furthermore, the top surface of the box body is an inclined surface, the box body is provided with an exhaust port, and the longitudinal height of the exhaust port is greater than the height of the liquid injection port.
Further, the phosphoric acid is packaged in a second inner bag, the second inner bag is placed in the box body, the second inner bag is fixedly communicated with the liquid injection port in a sealing mode, and the communication port is sealed by a collision sheet;
the second inner bag is fixedly communicated with an air outlet on the box body in a sealing way;
the box body is provided with a second air nozzle which is used for charging and discharging air into the box body.
Further, an air injection needle is fixed on the second inner bag;
the gas injection needle comprises a second needle tube, a second opening and a second closed ring;
the second needle tube is fixedly communicated with the second inner bag, and the tail end of the second needle tube is fixed with the inner wall of the air outlet;
the top end of the second needle tube is a closed tip, and a second opening is formed in the position, 2-4mm close to the bottom edge of the tip, of the second needle tube;
the second sealing ring is a rubber ring and can slide on the second needle tube, and the initial position of the second sealing ring can seal the second opening;
and the sealing sheet is sealed and fixed at the opening of the bottle body.
Furthermore, the air injection needle also comprises a floating block which is cylindrical and is internally provided with a through hole; the floating block can move upwards when the liquid level in the second needle tube rises, and the floating block is in sliding sealing fit with the inner wall of the second needle tube; the second opening can be sealed when the slider is moved to a top end limit position.
Further, the bottle body comprises an elastic sheet, a puncture needle, an inner bucket and a third air nozzle;
the bottom surface of the bottle body opposite to the opening is an elastic sheet, and a puncture needle is fixed at the center of the bottom surface in the elastic sheet;
the top of the inner bucket is towards the opening of the bottle body, and the bottom of the inner bucket is fixed with the inner wall of the bottle body in a sealing way; the needle tip of the puncture needle is abutted against the conical top; when the elastic sheet protrudes inwards the bottle body, the pricking pin can be driven to pierce the conical top;
the measuring component also comprises a third inner bag, the top opening of the third inner bag is fixedly connected with the conical bottom of the inner bucket in a sealing way, and the bottom opening of the third inner bag is fixedly communicated with the opening of the bottle body in a sealing way;
the bottle body is provided with a third air tap in the longitudinal range of the third inner bag; the third air tap is used for inflating and deflating the space between the third inner bag and the bottle body.
One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages: the detection device is convenient to use, full in reaction and fine and controllable in reaction process.
Drawings
FIG. 1 is a schematic view of a sampling assembly for aspirating cerebrospinal fluid;
FIG. 2 is a schematic diagram of the structure of the sampling assembly, the reaction assembly and the measuring assembly;
FIG. 3 is a schematic view of the sample injection assembly of the sampling assembly after injection into the reaction assembly;
FIG. 4 is an enlarged view of a portion of FIG. 2;
FIG. 5 is an enlarged view of a portion of FIG. 3;
FIG. 6 is a schematic view of a filling needle configuration;
FIG. 7 is a schematic view of the top surface of the case body being a slant surface;
FIG. 8 is a schematic view of a gas injection needle structure;
FIG. 9 is a schematic view showing a state where the slider moves upward to close the second opening when the liquid level in the air injection needle rises;
FIG. 10 is a schematic view of a measurement assembly;
FIG. 11 is a schematic view of the structure of the puncture needle after puncturing the inner funnel.
In the figure, a sampling assembly 100, a can body 110, an inner bag 120, a filling needle 130, a sampling nozzle 140,
A mounting nozzle 111, a top cover 112, an air nozzle 113,
Needle tube 131, opening 132, closed ring 133;
reaction assembly 200, cartridge 210, second inner bag 220, liquid injection port 230, gas vent 240, gas injection needle 250;
a second air nozzle 211, an abutting sheet 231, a second needle tube 251, a second opening 252, a second sealing ring 253 and a floating block 254;
a measuring assembly 300, a bottle body 310, a third inner bag 320 and a sealing sheet 330;
an elastic sheet 311, a puncture needle 312, an inner bucket 313 and a third air nozzle 314.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example one
A neurology body fluid sampling and detecting device comprises a sampling assembly 100, a reaction assembly 200 and a measuring assembly 300;
the sampling assembly 100 is used for quantitatively extracting cerebrospinal fluid; the reaction assembly 200 provides a reaction space for cerebrospinal fluid and phosphoric acid; the measuring assembly 300 provides a reaction space for hydrogen sulfide and sodium hydroxide, and is characterized in that,
the sampling assembly 100 comprises a tank 110, wherein the tank 110 is used for containing cerebrospinal fluid;
the reaction assembly 200 includes a cartridge body 210, wherein the top surface of the cartridge body 210 is communicated with the canister body 110, so that cerebrospinal fluid in the canister body 110 can flow into the cartridge body 210;
the measuring assembly 300 includes a bottle 310, and the bottle 310 is communicated with the top surface of the cartridge body 210 so that hydrogen sulfide gas can enter the bottle 310.
As shown in fig. 1-6, the operation is as follows:
step one, quantitative cerebrospinal fluid is filled into the tank body 110;
step two, the cup body 110 is communicated with the box body 210, so that cerebrospinal fluid flows into the box body 210;
and step three, communicating the bottle body 310 with the box body 210 to enable hydrogen sulfide to enter the bottle body, and injecting a quantitative sodium hydroxide solution into the bottle body 310 after the hydrogen sulfide is completely collected.
In the process, no complex operation is needed, and the use is convenient. And the gas is collected into the bottle body 310 first, and then the sodium hydroxide solution is added, so that the hydrogen sulfide and the sodium hydroxide can completely react.
Example two
To avoid air entering the reaction process and interfering with the reaction, the sampling assembly was further modified as shown in fig. 1-6.
The sampling assembly 100 further comprises an inner bag 120, a filling needle 130 and a sampling nozzle 140;
the inner bag 120 is a soft bag, one end of the inner bag is communicated with the tail end of the needle tube 131 of the filling needle 130, and the other end of the inner bag is communicated with the tail end of the sampling nozzle 140; the filling needle 130 and the sampling nozzle 140 are positioned at two opposite ends of the can body 110;
the filling needle 130 comprises a needle tube 131, an opening 132 and a closed ring 133;
the top end of the needle tube 131 is a tip end, and the tip end is a closed end; an opening 132 is formed in the needle tube 131, and the opening is close to the bottom edge of the tip end by 2-4 mm; the sealing ring 133 is a rubber ring, is sleeved on the needle tube 131 and can move on the needle tube 131 under the action of external force; the initial position of the closed ring 133 is sleeved at the opening 132, and the closed ring can close the opening 132;
the box body 210 is provided with a liquid injection port 230, an abutting sheet 231 is fixed in the liquid injection port 230, and the abutting sheet 231 is a rubber sheet; the contact piece 231 seals the liquid injection port 230;
the can 110 includes a mounting mouth 111 and a gas nozzle 113;
the tail end of the needle tube 131 is fixedly connected with the tank body 110, and the needle tube 131 is positioned in the mounting nozzle 111;
the mounting nozzle 111 can be detachably and fixedly communicated with the liquid injection port 230; when the mounting mouth 111 is fixed with the liquid injection port 230, the tip of the needle tube 131 passes through the abutting piece 231 and the opening 132 is also positioned below the abutting piece 231, and the sealing ring 133 is retained on the upper side of the abutting piece 231;
the air nozzle 113 is an air charging and discharging port on the tank 110.
When the cerebrospinal fluid sucking device is used, air in the tank body 110 is sucked through the air nozzle 113 by using the syringe, so that the cerebrospinal fluid can be sucked by the inner bag, the capacity of the inner bag can be fixed, and the tank body 110 is inverted after the inner bag is fully sucked. The mounting nozzle 111 is fixedly communicated with the liquid injection port 230 so that the needle tube 131 passes through the abutting piece 231 and the opening is positioned at the lower side of the abutting piece 231. Under the action of gravity, cerebrospinal fluid in the inner bag 120 flows downwards, and meanwhile, the sampling nozzle 140 is communicated with external air, so that the liquid can smoothly flow downwards. At the same time, it is ensured that air does not enter the phosphoric acid, since no additional pressure action is present.
The device also comprises a top cover 112, wherein the top cover 112 is detachably and fixedly connected with the opening of the mounting nozzle 111 and is used for sealing the opening of the mounting nozzle 111.
The sampling nozzle 140 is a tapered tube. The conical tube is convenient for pumping cerebrospinal fluid. The conical tube opening is provided with a rubber plug.
EXAMPLE III
Further modifications to the reaction assembly 200 are made to facilitate the entry of hydrogen sulfide gas into the body 310. As shown in fig. 7.
The top surface of the box body 210 is an inclined surface, and the longitudinal height of the exhaust port 240 is greater than the height of the liquid injection port 230. Allowing hydrogen sulfide gas to preferentially accumulate at the exhaust 240.
The phosphoric acid is packaged in the second inner bag 220, the second inner bag 220 is placed in the box body 210, the second inner bag 220 is fixedly communicated with the liquid injection port 230 in a sealing manner, and the communication port is sealed by the abutting sheet 231;
the second inner bag 220 is fixedly communicated with the air outlet 240 on the box body 210 in a sealing way;
the box body 210 is provided with a second air nozzle 211, and the second air nozzle 211 is used for filling and discharging air into the box body 210.
An air injection needle 250 is fixed on the second inner bag 220;
the gas injection needle 250 comprises a second needle tube 251, a second opening 252 and a second closing ring 253;
the second needle tube 251 is fixedly communicated with the second inner bag 220, and the tail end of the second needle tube 251 is fixed with the inner wall of the exhaust port 240;
the top end of the second needle tube 251 is a closed tip, and a second opening 252 is formed in the position, 2-4mm close to the bottom edge of the tip;
the second sealing ring 253 is a rubber ring and can slide on the second needle tube 251, and the initial position of the second sealing ring 253 can seal the second opening 252;
the opening of the bottle body 310 is sealed and fixed with a sealing sheet.
When the bottle body is inserted into the exhaust port 240, the second needle tube 251 passes through the sealing plate 330, the second sealing ring 253 slides downward, and the second opening 252 also enters the bottle body. Allowing hydrogen sulfide to enter the bottle 310.
The air injection needle 250 further comprises a floating block 254, wherein the floating block 254 is cylindrical and is internally provided with a through hole; the floating block 254 can move upwards when the liquid level in the second needle tube 251 rises, and the floating block 254 is in sliding sealing fit with the inner wall of the second needle tube 251; the second opening 252 can be sealed when the slider 254 is moved to a top end limit position.
The bottle body 310 comprises an elastic sheet 311, a puncture needle 312, an inner bucket 313 and a third air nozzle 314;
the bottom surface of the bottle body 310 opposite to the opening is an elastic sheet 311, and a puncture needle 312 is fixed at the center of the inner bottom surface of the elastic sheet 311;
the conical top of the inner bucket 313 faces the opening of the bottle body, and the conical bottom is fixed with the inner wall of the bottle body 310 in a sealing way; the needle tip of the puncture needle 312 is abutted against the conical top; when the elastic piece 311 protrudes inwards the bottle body 310, the puncture needle 312 can be driven to puncture the conical top;
the measuring component 300 further comprises a third inner bag 320, the top opening of the third inner bag 320 is fixedly connected with the conical bottom of the inner bucket 313 in a sealing way, and the bottom opening of the third inner bag 320 is fixedly communicated with the opening of the bottle body 310 in a sealing way;
the bottle body 310 is provided with a third air nozzle 314 within the longitudinal range of the third inner bag 320; the third air tap 314 is used for inflating and deflating the space between the third inner bag 320 and the bottle body 310.
In use, after the hydrogen sulfide is pumped into the third inner bag 320, the elastic sheet 311 is pressed to puncture the inner bucket 313, so that the sodium hydroxide enters the third inner bag 320.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, or improvement made without departing from the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims (9)

1. A neurology body fluid sampling and detecting device comprises a sampling component (100), a reaction component (200) and a measuring component (300);
the sampling assembly (100) is used for quantitatively extracting cerebrospinal fluid; the reaction component (200) provides a reaction space for cerebrospinal fluid and phosphoric acid; the measuring assembly (300) providing a reaction space for hydrogen sulphide and sodium hydroxide, characterized in that,
the sampling assembly (100) comprises a tank (110), wherein the tank (110) is used for containing cerebrospinal fluid;
the reaction assembly (200) comprises a box body (210), the top surface of the box body (210) is communicated with the tank body (110), so that cerebrospinal fluid in the tank body (110) can flow into the box body (210);
the measuring assembly (300) comprises a bottle body (310), the bottle body (310) is communicated with the top surface of the box body (210), and hydrogen sulfide gas can enter the bottle body (310);
the sampling assembly (100) further comprises an inner bag (120), a filling needle (130) and a sampling nozzle (140);
the inner bag (120) is a soft bag, one end of the inner bag is communicated with the tail end of a needle tube (131) of the filling needle (130), and the other end of the inner bag is communicated with the tail end of the sampling nozzle (140); the filling needle (130) and the sampling nozzle (140) are positioned at two opposite ends of the tank body (110);
the filling needle (130) comprises a needle tube (131), an opening (132) and a closed ring (133);
the top end of the needle tube (131) is a tip end, and the tip end is a closed end; an opening (132) is formed in the needle tube (131) and is close to the bottom edge of the tip end for 2-4 mm; the sealing ring (133) is a rubber ring and is sleeved on the needle tube (131), and the sealing ring can move on the needle tube (131) under the action of external force; the initial position of the closed ring (133) is sleeved at the opening (132) and can close the opening (132);
the box body (210) is provided with a liquid injection port (230), a contact resisting sheet (231) is fixed in the liquid injection port (230), and the contact resisting sheet (231) is a rubber sheet; the liquid injection port (230) is sealed by a contact piece (231);
the tank body (110) comprises a mounting nozzle (111) and an air nozzle (113);
the tail end of the needle tube (131) is fixedly connected with the tank body (110), and the needle tube (131) is positioned in the mounting nozzle (111);
the mounting nozzle (111) can be detachably and fixedly communicated with the liquid injection port (230); when the mounting nozzle (111) is fixed with the liquid injection port (230), the tip of the needle tube (131) penetrates through the abutting piece (231), the opening (132) is also positioned below the abutting piece (231), and the sealing ring (133) is retained on the upper side of the abutting piece (231);
the air tap (113) is an air charging and discharging port on the tank body (110).
2. The neurology body fluid sampling and detecting device of claim 1, wherein the tank body (110) further comprises a top cover (112), and the top cover (112) is detachably and fixedly connected with the opening of the mounting nozzle (111) and used for closing the opening of the mounting nozzle (111).
3. The neurological bodily fluid sampling testing device of claim 1 wherein the sampling nozzle (140) is a tapered tube.
4. The neurologic fluid sampling and detection device of claim 3, wherein the tapered tube port is provided with a rubber plug.
5. The neurologic body fluid sampling and detecting device as claimed in claim 1, wherein the top surface of the box body (210) is an inclined surface, the box body (210) is provided with an air outlet (240), and the longitudinal height of the air outlet (240) is greater than the height of the liquid injection port (230).
6. The neurologic body fluid sampling and detecting device according to claim 5, wherein the phosphoric acid is packaged in a second inner bag (220), the second inner bag (220) is placed in the box body (210), the second inner bag (220) is fixedly communicated with the liquid injection port (230) in a sealing manner, and the communication port is closed by an interference sheet (231);
the second inner bag (220) is fixedly communicated with an air outlet (240) on the box body (210) in a sealing way;
the box body (210) is provided with a second air nozzle (211), and the second air nozzle (211) is used for inflating and deflating the box body (210).
7. The neurologic body fluid sampling and detecting device of claim 6, wherein the second inner bag (220) is fixed with an air injection needle (250);
the gas injection needle (250) comprises a second needle tube (251), a second opening (252) and a second closed ring (253);
the second needle tube (251) is fixedly communicated with the second inner bag (220), and the tail end of the second needle tube (251) is fixed with the inner wall of the exhaust port (240);
the top end of the second needle tube (251) is a closed tip, and a second opening (252) is formed at the position 2-4mm close to the bottom edge of the tip;
the second closing ring (253) is a rubber ring and can slide on the second needle tube (251), and the second opening (252) can be closed at the initial position of the second closing ring (253);
the opening of the bottle body (310) is sealed and fixed with a sealing sheet.
8. The neurologic body fluid sampling and testing device of claim 7, wherein the insufflation needle (250) further comprises a slider (254), the slider (254) being cylindrical with a through hole therein; the floating block (254) can move upwards when the liquid level in the second needle tube (251) rises, and the floating block (254) is in sliding sealing fit with the inner wall of the second needle tube (251); the second opening (252) is capable of sealing when the slider (254) is moved to a top end limit position.
9. The neurologic body fluid sampling and detecting device of claim 8, wherein the bottle body (310) comprises an elastic sheet (311), a puncture needle (312), an inner bucket (313) and a third air tap (314);
the bottom surface of the bottle body (310) opposite to the opening is an elastic sheet (311), and a puncture needle (312) is fixed at the center of the inner bottom surface of the elastic sheet (311);
the conical top of the inner bucket (313) faces the opening of the bottle body, and the conical bottom is fixed with the inner wall of the bottle body (310) in a sealing way; the needle tip of the puncture needle (312) is butted against the conical top; when the elastic sheet (311) protrudes inwards the bottle body (310), the puncture needle (312) can be driven to puncture the conical top;
the measuring component (300) further comprises a third inner bag (320), the top opening of the third inner bag (320) is fixedly connected with the conical bottom of the inner bucket (313) in a sealing way, and the bottom opening of the third inner bag (320) is fixedly communicated with the opening of the bottle body (310) in a sealing way;
the bottle body (310) is provided with a third air tap (314) in the longitudinal range of the third inner bag (320); the third air tap (314) is used for charging and discharging air between the third inner bag (320) and the bottle body (310).
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CN104874093A (en) * 2015-04-24 2015-09-02 中山大学 Cerebrospinal fluid extracting device
CN105044090A (en) * 2015-06-24 2015-11-11 陕西师范大学 Sulfide detection method and device
CN107271596A (en) * 2017-07-12 2017-10-20 云南中烟工业有限责任公司 A kind of method of hydrogen sulfide content in hydrogen sulfide separator and detection medicinal extract spices
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