CN115519788B - Printing method, printing system, terminal and storage medium for tissue engineering scaffold - Google Patents
Printing method, printing system, terminal and storage medium for tissue engineering scaffold Download PDFInfo
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- 238000007639 printing Methods 0.000 title claims abstract description 109
- 238000000034 method Methods 0.000 title claims abstract description 45
- 238000003860 storage Methods 0.000 title claims abstract description 9
- 239000000835 fiber Substances 0.000 claims abstract description 77
- 238000001125 extrusion Methods 0.000 claims abstract description 69
- 238000004519 manufacturing process Methods 0.000 claims abstract description 60
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 12
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 12
- 230000006870 function Effects 0.000 claims description 11
- 230000003592 biomimetic effect Effects 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 241000254032 Acrididae Species 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 210000001519 tissue Anatomy 0.000 description 79
- 238000010146 3D printing Methods 0.000 description 22
- 239000011148 porous material Substances 0.000 description 9
- 230000006872 improvement Effects 0.000 description 8
- 238000013461 design Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 4
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
- B29C64/205—Means for applying layers
- B29C64/209—Heads; Nozzles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
- B29C64/245—Platforms or substrates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/30—Auxiliary operations or equipment
- B29C64/386—Data acquisition or data processing for additive manufacturing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y50/00—Data acquisition or data processing for additive manufacturing
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- Materials Engineering (AREA)
- Manufacturing & Machinery (AREA)
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Abstract
According to the printing method of the tissue engineering scaffold, provided by the embodiment of the application, the printing parameters are adjusted according to the variable fiber diameter bionic gradient scaffold model to obtain the tissue engineering scaffold model which accords with gradient expectation, and according to the tissue engineering scaffold model which accords with gradient expectation, a specific manufacturing code matched with the designed tissue engineering scaffold model is constructed, and according to writing rules of manufacturing codes of different printers, upgrading and reconstruction of a traditional extrusion type 3D printer can be completed, so that the tissue engineering scaffold can be used for preparing continuous variable fiber diameter scaffolds, and the production and manufacturing cost is reduced. In addition, the application also provides a printing system, a terminal and a storage medium of the tissue engineering scaffold.
Description
Technical Field
The invention relates to the technical field of biomedical engineering, in particular to a printing method, a printing system, a terminal and a storage medium of a tissue engineering bracket.
Background
Conventional extrusion 3D printing typically uses a commercial automated slicing tool (e.g., cura, simplify 3D) to automate the fiber filling of the target model, with the conventional filling pattern being an intra-layer fiber parallel arrangement, with an inter-layer fiber 90 ° angle. And then based on the writing rule of the printer manufacturing code, converting the printing path and printing parameters (such as the printing head moving speed, the extrusion air pressure, the barrel heating temperature, the extrusion head height and the like) obtained by the automatic slicing into the manufacturing code which can be identified by the extrusion type 3D printer, and calling the manufacturing code through control software of the printer and executing extrusion type 3D printing.
In the extrusion 3D printing process, the printing route (i.e., the 2D filling curve) determines the fiber distribution of the 3D printed sample, thereby affecting the pore structure of the sample. Traditional extrusion 3D printing, while allowing a user to fine tune relevant parameters of the automatic slicing, such as the spacing between 2D curves, the height between 3D fiber layers, etc., fundamentally makes it difficult to alter the default settings of their fiber filling patterns. Furthermore, while the diameter of the interlaminar fibers can be adjusted to achieve an axial gradient or the spacing of the fibers within the layers can be adjusted to achieve a linear horizontal gradient porosity to some extent, it is difficult to achieve a radial gradient, limiting the application and expansion of extrusion 3D printing techniques in gradient porosity scaffolds.
The new strategy of continuous variable fiber diameter extrusion 3D printing precisely controls the local porosity and pore size of the extruded 3D printed sample by adjusting the print speed of the print head throughout the print path and the extrusion head height. However, due to the automatic slicing encapsulation of the traditional extrusion type 3D printing, the user is limited to custom setting of printing parameters, and the development and the technical popularization of the continuous variable fiber diameter extrusion type 3D printing technology are severely limited. Therefore, how to build a continuous variable fiber diameter model parametric design and an extrusion type 3D printing bridge based on the extrusion type 3D printing technology is a key work to be accomplished urgently.
The prior art lacks to build a bridge manufactured by continuous variable fiber diameter model parameterized design and extrusion type 3D printing. Although literature (Diaz-Gomez L, kontoyiania P D, melshiori A J, et al, three-dimensional printing of tissue engineering scaffolds with horizontal pore and composition gradients [ J ]. Tissue Engineering Part C-Methods,2019,25 (7): 411-420) is based on conventional extrusion 3D printing techniques, radial gradient porosity is achieved by providing different fiber diameters and spacing in radially distinct regions, this approach is difficult to produce with high precision, continuous, controllable gradient porosity scaffolds.
Disclosure of Invention
In view of the foregoing, it is desirable to provide a printing method for tissue engineering scaffolds that can process samples of continuously variable fiber diameters, in order to address the problems of the prior art that it is difficult to prepare high-precision, continuous, controllable gradient pore scaffolds.
In order to achieve the above purpose, the present application adopts the following technical scheme:
one of the purposes of the application provides a printing method of a tissue engineering scaffold, which comprises the following steps:
obtaining a tissue engineering scaffold model conforming to gradient expectation;
constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model conforming to the gradient expectation;
and printing the tissue engineering scaffold according to the specific manufacturing codes.
In some embodiments, the step of obtaining the tissue engineering scaffold model meeting the gradient expectation specifically comprises the following steps:
constructing a functional relation between the printing speed and the fiber diameter;
constructing a variable fiber diameter bionic gradient stent model according to the required target gradient data;
and according to the variable fiber diameter bionic gradient stent model, adjusting printing parameters to obtain a tissue engineering stent model which accords with gradient expectation, wherein the printing parameters comprise a printing path, a printing speed and a fiber diameter.
In some of these embodiments, the step of constructing a function of the printing speed as a function of the fiber diameter specifically comprises the steps of:
based on the law of conservation of mass, a functional relationship of the printing speed and the fiber cross-sectional area is obtained:
wherein: v represents printing speed (mm/s), Q represents flow rate (mm) of beta-TCP/PCL ink extrusion 3 S, S represents the cross-sectional area (mm) of the beta-TCP/PCL fiber 2 )；
Circle-based area formula s=pi d 2 And/4, obtaining a function (v, d) of the printing speed as a function of the fiber diameter:
wherein: v represents printing speed (mm/s), Q represents flow rate (mm) of beta-TCP/PCL ink extrusion 3 S), d represents the approximate diameter (mm) of the β -TCP/PCL fiber.
In some embodiments, in the step of constructing the variable fiber diameter biomimetic gradient stent model according to the required target gradient data, the method specifically comprises the following steps:
a variable fiber diameter biomimetic gradient stent model based on three-dimensional design software is constructed from the desired target gradient data, including but not limited to Rhino, grasshopper.
In some embodiments, in the step of constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model meeting gradient expectation, the method specifically comprises the following steps:
reversely pushing out the printing speed corresponding to the tissue engineering scaffold model conforming to the gradient expectation based on the diameters of the fibers at all parts of the tissue engineering scaffold model conforming to the gradient expectation;
determining a matched extrusion head height in combination with the position of the fibers and the diameter of the fibers;
and constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to writing rules of the programming tool based on the manufacturing code of the extrusion type 3D printer.
In some of these embodiments, in the step of completing the preparation of the tissue engineering scaffold according to the specific manufacturing code, the method specifically comprises the following steps:
and calling the rewritten specific manufacturing code by using a control program of the extrusion type 3D printer, and controlling the three-coordinate printer to finish printing the tissue engineering scaffold.
In some of these embodiments, prior to the step of obtaining a tissue engineering scaffold model that meets gradient expectations, the steps of:
installing an extrusion head to work and prepare printing ink;
and (3) finishing the initialization processing of the three-coordinate printer based on the control program of the extrusion type 3D printer, wherein the initialization processing comprises zeroing of an XYZ three-coordinate motion system, preheating of a printing charging barrel and setting of extrusion pressure.
A second object of the present application is to provide a printing system for a tissue engineering scaffold, comprising:
a scaffold model acquisition unit for acquiring a tissue engineering scaffold model conforming to gradient expectation;
a specific manufacturing code generating unit for constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model conforming to gradient expectation;
and the printing unit is used for finishing the printing of the tissue engineering scaffold according to the specific manufacturing codes.
A third object of the present application provides a terminal comprising a processor, a memory coupled to the processor, wherein,
the memory stores program instructions for implementing the printing method of the tissue engineering scaffold of any one of the above;
the processor is configured to execute the program instructions stored by the memory to control printing of the tissue engineering scaffold.
A fourth object of the present application is to provide a storage medium storing program instructions executable by a processor for performing the printing method of the tissue engineering scaffold of any one of the above.
By adopting the technical scheme, the application has the following technical effects:
compared with the prior art, the application provides a printing method, a printing system, a terminal and a storage medium of a tissue engineering scaffold, and the printing method of the tissue engineering scaffold, which is provided by the embodiment of the application, adjusts printing parameters according to the variable fiber diameter bionic gradient scaffold model to obtain a tissue engineering scaffold model conforming to gradient expectation, constructs a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model conforming to gradient expectation, and can finish upgrading and reconstruction of a traditional extrusion type 3D printer according to writing rules of the manufacturing code of different printers, so that the tissue engineering scaffold can be used for preparing continuous variable fiber diameter scaffolds, and the production and manufacturing cost is reduced.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiments of the invention and together with the description serve to explain the invention and do not constitute a limitation on the invention. In the drawings:
fig. 1 is a flowchart of steps of a method for preparing a tissue engineering scaffold according to an embodiment of the present application.
Fig. 2 is a flowchart of steps for obtaining a tissue engineering scaffold model according to gradient expectation according to an embodiment of the present application.
FIG. 3 is a flowchart of the steps for constructing a specific manufacturing code matching a designed tissue engineering scaffold model according to an embodiment of the present application.
Fig. 4 is a schematic structural diagram of a printing system of a tissue engineering scaffold according to an embodiment of the present application.
Detailed Description
In order that those skilled in the art will better understand the present invention, a technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present invention and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the invention described herein may be implemented in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
Referring to fig. 1, according to an embodiment of the present invention, a printing method of a tissue engineering scaffold is provided, which includes the following steps S10 to S30, and each step is described in detail below.
Step S10: obtaining the tissue engineering scaffold model conforming to gradient expectation.
Referring to fig. 2, a flowchart of steps for obtaining a tissue engineering scaffold model according to gradient expectation provided in this embodiment specifically includes the following steps S11 to S13, and each step is described in detail below.
Step S11: and constructing a function relation of the printing speed and the fiber diameter.
As an improvement of the present application, a function relation of the printing speed and the fiber diameter is constructed, specifically including the following steps S111 to S112.
Step S111: based on the law of conservation of mass, a functional relationship of the printing speed and the fiber cross-sectional area is obtained:
wherein: v represents printing speed (mm/s), Q represents flow rate (mm) of beta-TCP/PCL ink extrusion 3 S, S represents the cross-sectional area (mm) of the beta-TCP/PCL fiber 2 )；
Step S112: circle-based area formula s=pi d 2 And/4, obtaining a function (v, d) of the printing speed as a function of the fiber diameter:
wherein: v denotes printing speed (mm/s), Q denotes beta-TCP/PCL inkFlow rate of water extrusion (mm) 3 S), d represents the approximate diameter (mm) of the β -TCP/PCL fiber.
The functional relationship between the printing speed and the fiber diameter is constructed by the above-mentioned S111 to S112.
Step S12: and constructing a variable fiber diameter bionic gradient stent model according to the required target gradient data.
As an improvement of the present application, in the step of constructing the variable fiber diameter biomimetic gradient stent model according to the required target gradient data, the method specifically comprises the following steps:
a variable fiber diameter biomimetic gradient stent model based on three-dimensional design software is constructed from the desired target gradient data, including but not limited to Rhino, grasshopper.
Step S13: and according to the variable fiber diameter bionic gradient stent model, adjusting printing parameters to obtain a tissue engineering stent model which accords with gradient expectation, wherein the printing parameters comprise a printing path, a printing speed and a fiber diameter.
It can be understood that a user can obtain a tissue engineering stent model which accords with gradient expectation by adjusting the fiber stacking pattern through adjusting printing parameters according to the required performance of the stent, breaks through the constraint that the diameters of filling fibers of the traditional extrusion type 3D printing model are the same, and provides technical support for preparing the controllable gradient pore stent by extrusion type 3D printing.
Step S120: and constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model conforming to the gradient expectation.
Referring to fig. 3, in the step of constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the gradient expectation, the following steps S121 to S123 are specifically included.
Step S121: and reversely pushing out the printing speed corresponding to the tissue engineering scaffold model conforming to the gradient expectation based on the diameter of the fiber at each place of the tissue engineering scaffold model conforming to the gradient expectation.
Step S122: a matched extrusion head height is determined in combination with the position of the fibers and the diameter of the fibers.
Step S123: and constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to writing rules of the programming tool based on the manufacturing code of the extrusion type 3D printer.
As an improvement of the present application, the extrusion type 3D printer includes, but is not limited to, a strapdown RegenovoWS。
As another improvement of the present application, the extrusion type 3D printing includes, but is not limited to, normal temperature extrusion type 3D printing, low temperature freezing extrusion type 3D printing.
As another improvement of the present application, the printing ink of the extrusion type 3D printer includes, but is not limited to, β -TCP/PCL.
As another improvement of the present application, the manufacturing code writing rules of the extrusion type 3D printer write programming tools used for manufacturing codes for which the design model matches, including but not limited to GHPython.
It can be appreciated that according to the variable fiber diameter bionic gradient stent model, the printing parameters are adjusted to obtain a tissue engineering stent model which accords with gradient expectation so as to obtain a manufacturing code matched with a design model.
Step S30: and printing the tissue engineering scaffold according to the specific manufacturing codes.
As an improvement of the present application, in the step of completing the preparation of the tissue engineering scaffold according to the specific manufacturing code, the method specifically comprises the following steps: and calling the rewritten specific manufacturing code by using a control program of the extrusion type 3D printer, and controlling the three-coordinate printer to finish printing the tissue engineering scaffold.
As another improvement of the present application, before the step of obtaining the tissue engineering scaffold model conforming to the gradient expectation, the method further comprises the following steps:
step S40: the mounting of the extrusion head works and prepares the printing ink.
Step S50: and (3) finishing the initialization processing of the three-coordinate printer based on the control program of the extrusion type 3D printer, wherein the initialization processing comprises zeroing of an XYZ three-coordinate motion system, preheating of a printing charging barrel and setting of extrusion pressure.
According to the printing method of the tissue engineering scaffold, provided by the embodiment of the application, the printing parameters are adjusted according to the variable fiber diameter bionic gradient scaffold model to obtain a tissue engineering scaffold model which accords with gradient expectation, and according to the tissue engineering scaffold model which accords with gradient expectation, a specific manufacturing code matched with the designed tissue engineering scaffold model is constructed, and according to writing rules of manufacturing codes of different printers, upgrading and reconstruction of a traditional extrusion type 3D printer can be completed, so that the printing method can be used for preparing a continuous variable fiber diameter scaffold, and production and manufacturing cost is reduced. The tissue engineering scaffold obtained by the method can be applied to the fields of bone tissue engineering, meniscus tissue engineering, vascular tissue engineering and the like which have the need for gradient pores, and has wide application.
Referring to fig. 4, a schematic structural diagram of a printing system of a tissue engineering scaffold according to an embodiment of the present application includes: a scaffold model acquisition unit 110 for acquiring a tissue engineering scaffold model conforming to gradient expectation; a specific manufacturing code generating unit 120, configured to construct a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model conforming to the gradient expectation; and a printing unit 130 for completing the printing of the tissue engineering scaffold according to the specific manufacturing code.
The detailed implementation scheme of the printing system for the tissue engineering scaffold provided in the embodiment is described in detail in the above embodiment, and will not be described here again.
According to the printing system of the tissue engineering scaffold, provided by the embodiment of the application, the printing parameters are adjusted according to the variable fiber diameter bionic gradient scaffold model to obtain the tissue engineering scaffold model which accords with gradient expectation, and according to the tissue engineering scaffold model which accords with gradient expectation, a specific manufacturing code matched with the designed tissue engineering scaffold model is constructed, and according to writing rules of manufacturing codes of different printers, upgrading and reconstruction of a traditional extrusion type 3D printer are completed, so that the printing system can be used for preparing continuous variable fiber diameter scaffolds, and production and manufacturing cost is reduced.
The application also provides a terminal comprising a processor and a memory coupled with the processor, wherein the memory stores program instructions for implementing the printing method of the tissue engineering scaffold of any one of the above; the processor is configured to execute the program instructions stored by the memory to control printing of the tissue engineering scaffold.
The application also provides a storage medium, and the storage medium stores a program file capable of realizing the printing method of the tissue engineering scaffold provided by any one of the above.
The technical advantages of the embodiments of the present invention are at least: according to the variable fiber diameter bionic gradient stent model, printing parameters are adjusted to obtain a tissue engineering stent model conforming to gradient expectation, and according to the tissue engineering stent model conforming to gradient expectation, a specific manufacturing code matched with the designed tissue engineering stent model is constructed, and according to writing rules of manufacturing codes of different printers, upgrading and reconstruction of a traditional extrusion type 3D printer can be completed, so that the continuous variable fiber diameter stent can be prepared, and the production and manufacturing cost is reduced.
The above technical scheme is described in detail below with reference to specific embodiments. The specific implementation cases are as follows:
example 1:
A. the invention introduces a new extrusion type 3D printing strategy of continuously controllable fiber diameter with variable printing speed and variable extrusion head height based on the traditional extrusion type 3D printing technology. It achieves a gradient pore structure by precisely controlling the variation of the diameter of the filling fibers throughout, which requires that the fibers distributed throughout the model should have a print speed and extrusion head height corresponding thereto to ensure that the print speed and extrusion head height remain consistent with the fiber diameter.
B. beta-TCP (Sigma-Aldrich, USA) and PCL (Aldrich, USA) with a molecular weight of 14000 were selected and mixed at a weight ratio of 25% (w/w) to prepare a beta-TCP/PCL ink material. First, they were placed in a heating cabinet at 72℃for 1 hour, and then stirred uniformly with a spoon at room temperature, and this procedure was repeated 3 times.
C. Regenovo produced by Jienof company of ChinaAs a hardware platform for the research, the WS extrusion type 3D printer can realize XYZ three-coordinate axis linkage, meets the motion control requirement of the invention on the printing speed and the extrusion head height, and can obtain the writing rule of the printer manufacturing code. In addition, the control software matched with the extrusion type 3D printer is Bio-architecture.
D. The print parameters are determined as follows: extrusion air pressure (400 kPa), extrusion head size (400 μm), heating temperature (72 ℃), ink material, and other printing parameters.
E. In order to realize parameterized design and variable fiber diameter extrusion type 3D printing preparation of the controllable gradient tissue engineering scaffold, gradient pores of the scaffold can be adjusted in a one-key mode, manufacturing codes matched with a design model are obtained, and a bridge based on variable fiber diameter design and extrusion type 3D printer of software rho and Grasshopper is built.
F. Regenovo from the base Yu Jienuo femtoThe WS extrusion type 3D printer makes the writing rule of the code, and the printing path, the printing speed matched with the path and the extrusion head height are converted into motion control instructions which can be recognized by printing equipment by means of a programming tool GHPython of Grasshopper. Finally, the preparation of the variable fiber diameter extrusion type 3D printing gradient pore tissue engineering scaffold is completed through an extrusion type 3D printer, the prepared beta-TCP/PCL ink and the rewritten manufacturing code.
The invention is proved to be feasible through experimental verification.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (8)
1. A method for printing a tissue engineering scaffold, comprising the steps of:
obtaining a tissue engineering scaffold model conforming to gradient expectation;
constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model conforming to the gradient expectation;
printing the tissue engineering scaffold according to the specific manufacturing codes;
the method specifically comprises the following steps of:
constructing a functional relation between the printing speed and the fiber diameter;
constructing a variable fiber diameter bionic gradient stent model according to the required target gradient data;
according to the variable fiber diameter bionic gradient stent model, adjusting printing parameters to obtain a tissue engineering stent model conforming to gradient expectation, wherein the printing parameters comprise a printing path, a printing speed and a fiber diameter;
the step of constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model meeting gradient expectation specifically comprises the following steps:
reversely pushing out the printing speed corresponding to the tissue engineering scaffold model conforming to the gradient expectation based on the diameters of the fibers at all parts of the tissue engineering scaffold model conforming to the gradient expectation;
determining a matched extrusion head height in combination with the position of the fibers and the diameter of the fibers;
and constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to writing rules of the programming tool based on the manufacturing code of the extrusion type 3D printer.
2. The method for printing a tissue engineering scaffold according to claim 1, wherein in the step of constructing a function of printing speed and fiber diameter, the method comprises the steps of:
based on the law of conservation of mass, a functional relationship of the printing speed and the fiber cross-sectional area is obtained:
wherein: v represents the printing speed mm/s, Q represents the flow mm of the beta-TCP/PCL ink extrusion 3 S, S represent the cross-sectional area mm of the beta-TCP/PCL fiber 2 ；
Circle-based area formula s=pi d 2 And/4, obtaining a function (v, d) of the printing speed as a function of the fiber diameter:
wherein: v represents the printing speed mm/s, Q represents the flow mm of the beta-TCP/PCL ink extrusion 3 And/s, d represent the approximate diameter mm of the β -TCP/PCL fiber.
3. The method for printing a tissue engineering scaffold according to claim 2, wherein in the step of constructing a variable fiber diameter biomimetic gradient scaffold model according to the required target gradient data, the method specifically comprises the following steps:
a variable fiber diameter biomimetic gradient stent model based on three-dimensional design software is constructed from the desired target gradient data, including but not limited to Rhino, grasshopper.
4. The method of printing a tissue engineering scaffold according to claim 1, wherein in the step of completing the preparation of the tissue engineering scaffold according to the specific manufacturing code, the method specifically comprises the steps of:
and calling the rewritten specific manufacturing code by using a control program of the extrusion type 3D printer, and controlling the three-coordinate printer to finish printing the tissue engineering scaffold.
5. The method of printing a tissue engineering scaffold according to claim 1, further comprising the steps of, prior to the step of obtaining a gradient-expected tissue engineering scaffold model:
installing an extrusion head to work and prepare printing ink;
and (3) finishing the initialization processing of the three-coordinate printer based on the control program of the extrusion type 3D printer, wherein the initialization processing comprises zeroing of an XYZ three-coordinate motion system, preheating of a printing charging barrel and setting of extrusion pressure.
6. A printing system for a tissue engineering scaffold according to the printing method for a tissue engineering scaffold of claim 1, comprising:
a scaffold model acquisition unit for acquiring a tissue engineering scaffold model conforming to gradient expectation;
a specific manufacturing code generating unit for constructing a specific manufacturing code matched with the designed tissue engineering scaffold model according to the tissue engineering scaffold model conforming to gradient expectation;
and the printing unit is used for finishing the printing of the tissue engineering scaffold according to the specific manufacturing codes.
7. A terminal comprising a processor, a memory coupled to the processor, wherein,
the memory stores program instructions for implementing the printing method of the tissue engineering scaffold of any one of claims 1-5;
the processor is configured to execute the program instructions stored by the memory to control printing of the tissue engineering scaffold.
8. A storage medium storing program instructions executable by a processor for performing the method of printing a tissue engineering scaffold according to any one of claims 1-5.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211082304.8A CN115519788B (en) | 2022-09-06 | 2022-09-06 | Printing method, printing system, terminal and storage medium for tissue engineering scaffold |
| PCT/CN2022/137709 WO2024051013A1 (en) | 2022-09-06 | 2022-12-08 | Printing method and printing system for tissue engineering scaffold, terminal, and storage medium |
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| CN115519788A (en) | 2022-12-27 |
| WO2024051013A1 (en) | 2024-03-14 |
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