CN115517254B

CN115517254B - Efficient attractant for myxoplasma gondii and application thereof

Info

Publication number: CN115517254B
Application number: CN202211266965.6A
Authority: CN
Inventors: 王桂荣; 王婵
Original assignee: Agricultural Genomics Institute at Shenzhen of CAAS
Current assignee: Agricultural Genomics Institute at Shenzhen of CAAS
Priority date: 2022-10-17
Filing date: 2022-10-17
Publication date: 2024-03-19
Anticipated expiration: 2042-10-17
Also published as: CN115517254A

Abstract

The invention relates to a high-efficiency attractant for myxoplasma gondii and application thereof. Wherein, cis-11-hexadecene acetate and/or peach aldehyde can increase the number of the myxoplasma gondii sex pheromone induced myxoplasma gondii adults. The high-efficiency attractant for the myxoplasma gondii comprises myxoplasma gondii sex pheromone and cis-11-hexadecene acetate; further, peach aldehyde can be included.

Description

Efficient attractant for myxoplasma gondii and application thereof

Technical Field

The invention relates to the field of biological control, in particular to a synergistic compound of myxoplasma gondii sex pheromone.

Background

Sex pheromone is a trace chemical substance for the amphiprotic communication of insects, is released by female moths' gonads, and has an attracting effect on remote male moths. Sex pheromones have species specificity, and the components and proportions released by female moths of different species are different, so that reproductive isolation is ensured. The artificial preparation of a mixture of sex pheromone compounds released by female moths of a certain species and the proportion thereof generally has better attraction effect on male moths of the same species. Therefore, sex attractant prepared by compounding sex pheromone with slow-release carrier can trap a large amount of target male moths and has population monitoring effect on migratory pests, so that the sex attractant is widely used for preventing and controlling most of agriculture and forestry pests.

Mythimna loreyi (duponche), a species of noctuid belonging to the genus Lepidoptera (Lepidoptera) genus noctuid belonging to the genus noctuid, is a global migratory pest distributed in africa, australia, middle east, asia, and in guangdong, guangxi, jiangsu, henan, yunnan, sichuan, fujian, shandong, zhejiang, hunan, hubei, and Jiangxi in China. The larvae have the characteristics of omnivorous and violent feeding, and gramineous plants such as corn, rice, wheat, sorghum, sugarcane and the like are favored to eat, and can cause serious yield reduction to crops due to migration and outbreak. The list of crop diseases and insect pests is listed by agricultural rural department in 2020.

At present, the myxoplasma gondii is mainly controlled by chemical pesticides in China, has certain defects, is not suitable for long-term green prevention and control, and lacks sex attractant products capable of trapping and monitoring a large number of myxoplasma gondii in the market.

Disclosure of Invention

The invention provides an application of cis-11-hexadecene acetate and/or peach aldehyde in improving the number of the myxoplasma gondii sex pheromone induced myxoplasma gondii adults.

In a specific embodiment, the myxoplasma gondii is male myxoplasma gondii adults.

The second invention provides a composition comprising a myxoplasma gondii sex pheromone and cis-11-hexadecene acetate.

In a specific embodiment, the mass ratio of the myxoplasma gondii sex pheromone to the cis-11-hexadecene acetate is (104.4 to 110.5): (5 to 19.7).

In a specific embodiment, peach aldehyde is also included in the composition.

In a specific embodiment, the mass ratio of the myxoplasma gondii sex pheromone, the cis-11-hexadecene acetate and the peach aldehyde is (104.4 to 110.5): (5 to 19.7): (5 to 15).

In one embodiment, the myxoplasma gondii sex pheromones are cis-9-tetradecene acetate and cis-7-dodecene acetate.

In a specific embodiment, the mass ratio of said cis-9-tetradecene acetate to said cis-7-dodecene acetate is 1000: (44 to 105).

In a specific embodiment, a solvent is also included in the composition.

In one embodiment, the solvent is hexane, such as n-hexane.

In one embodiment, the content of cis-9-tetradecene acetate, cis-7-dodecene acetate, cis-11-hexadecene acetate and peach aldehyde in the organic solvent is independently from 5 to 15 micrograms/microliter, for example 10 micrograms/microliter.

The present invention also provides a lure comprising a carrier and a composition according to any one of the second aspects of the invention, wherein the composition is adsorbed onto the carrier,

in one embodiment, the carrier is a rubber stopper.

The invention has the beneficial effects that:

the invention discovers that the cis-11-hexadecene acetate and the peach aldehyde can not be used for attracting the adult myxoworm of the Lawsonia independently for the first time, but the cis-11-hexadecene acetate can obviously improve the quantity of the adult myxoworm of the Lawsonia induced by the sex pheromone of the Lawsonia; the peach aldehyde can obviously improve the effect of the composition of the myxoplasma gondii sex pheromone and the cis-11-hexadecene acetate on attracting the myxoplasma gondii adults.

Therefore, the myxoplasma gondii sex pheromone attractant added with cis-11-hexadecene acetate or further added with peach aldehyde can improve the trapping effect of the myxoplasma gondii, has high sensitivity, can further reduce the number of field male adults, reduces the number of offspring insect ports, and has important application value for monitoring and controlling the myxoplasma gondii which is an important agricultural pest.

Detailed Description

The above-described aspects of the invention are described in further detail below in the form of preferred embodiments, which are not to be construed as limiting the invention.

Reagents for use in the examples of the invention are commercially available unless otherwise specified.

Cis-9-tetradecene acetate and cis-7-dodecene acetate are components of myxoplasma gondii sex pheromone.

Example 1

Test of the attractant effect of cis-11-hexadecene acetate on myxoma Latifolia

Test time and place: 28 days to 7 days of 8 months to 9 months in 2022, yang Lingou in the salt-Yang City of Shanxi province, and the crops planted in the field are summer corns.

The preparation method of the lure comprises the following steps: cis-9-tetradecene acetate, cis-7-dodecene acetate and cis-11-hexadecene acetate were prepared as mother solutions having a concentration of 10. Mu.g/μl, respectively, using n-hexane as a solvent.

The formulation of the myxoplasma gondii sex pheromone is used as a first positive control: 100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate, 4.4. Mu.l of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the above-mentioned mother liquor, and these were thoroughly mixed, and then, the mixture was dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure having a total dose of 1.044 mg of both, which was designated as P-CK-1.

The formulation of the myxoplasma gondii sex pheromone was used as a second positive control: 100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate, 8.8. Mu.l of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the above-mentioned mother liquor, and these were thoroughly mixed, and then, the mixture was dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure having a total dose of 1.088 mg of both, which was designated as P-CK-2.

The formulation of the myxoplasma gondii sex pheromone is used as a third positive control: 100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate, 10.5. Mu.l of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the above-mentioned mother liquor, and these were thoroughly mixed, and then, the mixture was dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure having a total dose of 1.105 mg of both, which was designated as P-CK-3.

100. Mu.l of N-hexane was added dropwise to a green rubber stopper (from Mitsubishi tetragonal biotechnology) of 240mm by 340mm size using N-hexane as a negative control, to prepare a lure, and this was designated as N-CK.

100. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken and dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the dosage of cis-11-hexadecene acetate was 1 mg, which was designated as L1.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the mother liquor, 4.4. Mu.l of the 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the mother liquor, 5. Mu.l of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the mother liquor, the three were thoroughly mixed, and then the mixture was dropped onto a green rubber plug having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the three was 1.094 mg, which was designated as L2.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the mother liquor, 4.4. Mu.l of the 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the mother liquor, 10. Mu.l of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the mother liquor, the three were thoroughly mixed, and then the mixture was dropped onto a green rubber plug having a size of 240 mm.times.340 mm to prepare a lure, the total dose of the three was 1.144 mg, and the lure was designated as L3.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the mother liquor, 4.4. Mu.l of the 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the mother liquor, 19.7. Mu.l of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the mother liquor, and the three were thoroughly mixed and then dropped onto a green rubber plug having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the three was 1.241 mg, which was designated as L4.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the mother liquor, 8.8. Mu.l of the 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the mother liquor, 5. Mu.l of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the mother liquor, the three were thoroughly mixed, and then the mixture was dropped onto a green rubber plug having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the three was 1.138 mg, which was designated as L5.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the mother liquor of the 10. Mu.g/. Mu.l of cis-7-dodecene acetate, 8.8. Mu.l of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the mother liquor of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate, the three were thoroughly mixed and then dropped onto a green rubber plug having a size of 240 mm.times.340 mm to prepare a lure, the total dose of the three was 1.188 mg, which was designated as L6.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the mother liquor, 8.8. Mu.l of the 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the mother liquor, 19.7. Mu.l of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the mother liquor, and the three were thoroughly mixed and then dropped onto a green rubber plug having a size of 240 mm.times.340 mm to prepare a lure, so that the total dose of the three was 1.285 mg, which was designated as L7.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate, 10.5. Mu.l of the above-mentioned 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the above-mentioned 10. Mu.g/. Mu.l of cis-11-hexadecene acetate, and the three were thoroughly mixed and then dropped onto a green rubber plug having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the three was 1.155 mg, which was designated as L8.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the above-mentioned 10. Mu.g/. Mu.l of cis-7-dodecene acetate, 10.5. Mu.l of the above-mentioned 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the above-mentioned 10. Mu.g/. Mu.l of cis-11-hexadecene acetate, and the three were thoroughly mixed and then dropped onto a green rubber plug having a size of 240 mm.times.340 mm to prepare a lure, so that the total dose of the three was 1.205 mg, which was designated as L9.

100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out of the mother liquor, 8.8. Mu.l of the 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out of the mother liquor, 19.7. Mu.l of the 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out of the mother liquor, and the three were thoroughly mixed and then dropped onto a green rubber plug having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the three was 1.302 mg, which was designated as L10.

The positive control, negative control, and components and amounts of L1 to L10 are shown in table 1.

TABLE 1

Test fields of 20 mu are selected and divided into 14 treatment groups, and each treatment group is repeated for 3. The traps were 20 cm above the maize plants, each lure was individually placed in one trap device with 15 meters spacing between each trap, and a total of 3 rows of 14 different treated lures were randomly placed in each row, P-CK-1, P-CK-2, P-CK-3, N-CK, L1 to L10. The trapping amount of the myxoplasma gondii adults and the sex of the trapped adults in the trap are counted once a day, then the trapped myxoplasma gondii adults are emptied from the trap, and then the positions of the traps treated differently in each row are randomly exchanged to eliminate test errors. Total insect trapping amounts of the individual lures in the 2022 8-9-7 hanging period for 11 days were counted for 3 replicates, and the average of 3 replicates was calculated, with significant differences using one-way ANOVA variance analysis, α=0.05, and the results are shown in table 2.

From the results in Table 2, it was found that the N-CK lures and L1 lures did not trap the myxoplasma gondii adults; the number of the myxoma rouxii adults induced by the P-CK-1 induced core and the P-CK-2 induced core is equal, the number of the myxoma rouxii adults induced by the P-CK-3 induced core is slightly higher than that of the P-CK-1 induced core and the P-CK-2 induced core, but the number of the myxoma rouxii adults induced by the three positive controls is smaller; the quantity of the L2 lure is equal to that of the myxoma strain adults lured by the L3 lure; the quantity of the L3 lure, the L4 lure, the L5 lure, the L6 lure and the L8 lure are equivalent to each other; the quantity of the L4 lure, the L6 lure and the L9 lure are equivalent to each other; the L10 lures the most of the number of the myxoma rouxii adults lured, the significance is higher than the rest 13 lures, and the lures from the L2 lures to the L10 lures are all significantly more than the P-CK-1 lures, the P-CK-2 lures and the P-CK-3 lures.

The above results fully demonstrate that cis-11-hexadecene acetate alone is not effective in attracting male adults of myxoworm, when the mass ratio of myxoworm sex pheromone (both cis-9-tetradecene acetate and cis-7-dodecene acetate) to cis-11-hexadecene acetate is (104.4 to 110.5): (5 to 19.7), the cis-11-hexadecene acetate can obviously increase the attracting effect of the myxoma strain sex pheromone on male adults of the myxoma strain, thereby improving the trapping effect of the myxoma strain sex attractant.

TABLE 2

Treatment group	Average trapping quantity (head/trap)
		P-CK-1	26g
P-CK-2	55fg
		P-CK-3	72f
N-CK	0g
		L1	0g
L2	141e
		L3	177de
L4	233cd
		L5	191d
L6	242cd
		L7	335b
L8	204d
		L9	257c
L10	382a

Note that: significant differences between the different treatments are indicated by letters a to g.

Example 2

Experiment of the effect of peach aldehyde on attracting myxoplasma gondii

Test time and place: the planting crop in the field is summer corn in the city Yang Lingou of salty yang of Shanxi province from 8 days 9 months 2022 to 25 days 9 months.

The lure core configuration method comprises the following steps: peach aldehyde is prepared into mother solution with concentration of 10 microgram/microliter by taking normal hexane as a solvent.

The L2, L4, L5, L7, L8 and L10 formulations in example 1 were formulated.

100. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was taken and dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, and the peach aldehyde dose was 1 mg and was designated as L11.

To the L2 formulation of example 1, 1. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then, the mixture was dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the four was 1.104 mg, which was designated as L12.

To the L2 formulation of example 1, 5. Mu.l of a 10. Mu.g/. Mu.l of a mother liquor of peach aldehyde was added, and the mixture was thoroughly mixed, and then, a green rubber plug having a size of 240 mm. Times.340 mm was dropped to prepare a lure, so that the total dose of the four was 1.144 mg, which was designated as L13.

To the L2 formulation of example 1, 15. Mu.l of a 10. Mu.g/mu.l of a mother liquor of peach aldehyde was added, mixed thoroughly, and then dropped onto a 240 mm. Times.340 mm sized green rubber plug to prepare a lure, so that the total dose of the four was 1.244 mg, which was designated as L14.

To the L4 formulation of example 1, 1. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then, the mixture was dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the four was 1.251 mg, which was designated as L15.

To the L4 formulation of example 1, 5. Mu.l of a 10. Mu.g/mu.l of a mother liquor of peach aldehyde was added, and the mixture was thoroughly mixed, and then, a green rubber plug having a size of 240 mm. Times.340 mm was dropped to prepare a lure, so that the total dose of the four was 1.291 mg, which was designated as L16.

To the L4 formulation of example 1, 15. Mu.l of a 10. Mu.g/mu.l of a mother liquor of peach aldehyde was added, and the mixture was thoroughly mixed, and then, dropped onto a 240 mm. Times.340 mm-sized green rubber plug to prepare a lure, so that the total dose of the four was 1.391 mg, which was designated as L17.

To the L5 formulation of example 1, 1. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the four was 1.148 mg, which was designated as L18.

To the L5 formulation of example 1, 5. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the four was 1.188 mg, which was designated as L19.

To the L5 formulation of example 1, 15. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the four was 1.288 mg, which was designated as L20.

To the L7 formulation of example 1, 1. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then, the mixture was dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the total dose of the four was 1.295 mg, which was designated as L21.

To the L7 formulation of example 1, 5. Mu.l of a 10. Mu.g/mu.l of a mother liquor of peach aldehyde was added, and the mixture was thoroughly mixed, and then, a green rubber plug having a size of 240 mm. Times.340 mm was dropped to prepare a lure, so that the total dose of the four was 1.335 mg, which was designated as L22.

To the L7 formulation of example 1, 15. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then dropped onto a green rubber stopper having a size of 240mm X340 mm to prepare a lure, so that the total dose of the four was 1.435 mg, which was designated as L23.

To the L8 formulation of example 1, 1. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then dropped onto a green rubber stopper having a size of 240mm X340 mm to prepare a lure, so that the total dose of the four was 1.165 mg, which was designated as L24.

To the L8 formulation of example 1, 5. Mu.l of a 10. Mu.g/mu.l of a mother liquor of peach aldehyde was added, mixed thoroughly, and then dropped onto a 240 mm. Times.340 mm sized green rubber plug to prepare a lure, so that the total dose of the four was 1.205 mg, designated as L25.

To the L8 formulation of example 1, 15. Mu.l of a 10. Mu.g/mu.l of a mother liquor of peach aldehyde was added, and the mixture was thoroughly mixed, and then, dropped onto a 240 mm. Times.340 mm-sized green rubber plug to prepare a lure, so that the total dose of the four was 1.305 mg, which was designated as L26.

To the L10 formulation of example 1, 1. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then dropped onto a 240 mm. Times.340 mm size green rubber plug to prepare a lure, so that the total dose of the four was 1.312 mg, which was designated as L27.

To the L10 formulation of example 1, 5. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, mixed thoroughly, and then dropped onto a 240 mm. Times.340 mm size green rubber plug to prepare a lure, so that the total dose of the four was 1.352 mg, designated as L28.

To the L10 formulation of example 1, 15. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of peach aldehyde was added, and the mixture was thoroughly mixed, and then dropped onto a green rubber stopper having a size of 240mm X340 mm to prepare a lure, so that the total dose of the four was 1.452 mg, which was designated as L29.

The components and amounts of L2, L4, L5, L7, L8 and L10 to L29 are shown in Table 3.

TABLE 3 Table 3

Test fields of 20 mu are selected and divided into 25 treatment groups, and each treatment group is repeated for 3.

Otherwise, the same as in example 1 was conducted.

The experimental results are shown in Table 4.

TABLE 4 Table 4

From the results in Table 4, L11 lures that no myxoplasma gondii adults were lured; the quantity of the myxoma rouxii adults induced and caught by the L13 induced core and the L14 induced core is obviously higher than that of the L12 induced core and the L2 induced core; the quantity of the myxoma rouxii adults induced and caught by the L16 induced core and the L17 induced core is obviously higher than that of the L15 induced core and the L4 induced core; the quantity of the myxoma rouxii adults induced and caught by the L19 induced core and the L20 induced core is equivalent to that of the L18 induced core and the L5 induced core; the quantity of the myxoma rouxii adults induced and caught by the L22 induced core and the L23 induced core is equivalent to that of the myxoma rouxii adults which are obviously higher than that of the L21 induced core and the L7 induced core; the quantity of the myxoma rouxii adults induced and caught by the L25 induced core and the L26 induced core is equivalent to that of the myxoma rouxii adults which are obviously higher than that of the L24 induced core and the L8 induced core; the quantity of the myxoma rouxii adults induced and caught by the L28 lure and the L29 lure is obviously higher than that of the L27 lure and the L10 lure. Wherein, the L29 lures the most of the myxoma strain adults that the core lures.

The above results fully demonstrate that peach aldehyde alone is not effective in attracting myxoplasma gondii adults when the myxoplasma gondii sex pheromones (cis-9-tetradecene acetate and cis-7-dodecene acetate), cis-11-hexadecene acetate and peach aldehyde are in a mass ratio of (104.4 to 110.5): (5 to 19.7): and (5) to 15), the peach aldehyde can obviously increase the attracting effect of the composition of the myxoma strain sex pheromone and the cis-11-hexadecene acetate on the myxoma strain adults, so that the attracting amount of the myxoma strain sex attractant is increased.

Claims

1. The application of cis-11-hexadecene acetate or the combination of cis-11-hexadecene acetate and peach aldehyde in improving the number of the myxoplasma gondii sex pheromone induced myxoplasma gondii adults,

the myxoplasma gondii sex pheromones are cis-9-tetradecene acetate and cis-7-dodecene acetate;

the mass ratio of the cis-9-tetradecene acetate to the cis-7-dodecene acetate is 1000: (44 to 105).

2. The use according to claim 1, wherein the myxoworm is a male myxoworm strain adult.

3. A composition comprising a myxoplasma gondii sex pheromone and cis-11-hexadecene acetate;

the mass ratio of the cis-9-tetradecene acetate to the cis-7-dodecene acetate is 1000: (44 to 105);

the mass ratio of the myxoplasma gondii sex pheromone and the cis-11-hexadecene acetate is (104.4 to 110.5): (5 to 19.7).

4. A composition according to claim 3, further comprising peach aldehyde.

5. The composition of claim 4, wherein the mass ratio of the myxoplasma gondii sex pheromone, the cis-11-hexadecene acetate and the peach aldehyde is (104.4 to 110.5): (5 to 19.7): (5 to 15).

6. The composition according to any one of claims 3 to 5, further comprising a solvent.

7. The composition of claim 6, wherein the solvent is hexane.

8. A lure comprising a carrier and a composition according to any one of claims 3 to 7, wherein the composition is adsorbed onto the carrier.

9. The lure according to claim 8, wherein the carrier is a rubber plug.